CN114365658B - Lentinus edodes fruiting body model cultivation method - Google Patents
Lentinus edodes fruiting body model cultivation method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a mushroom fruiting body model cultivation method, which comprises the following steps: (1) one-time inoculation field planting corn stalk: inoculating the lentinus edodes mycelium inoculation block to the surface of a tiled fresh corn straw slice, culturing in a breathable closed sterile environment, controlling the humidity of the culture environment to be more than 80%, and culturing at room temperature in a dark place until the lentinus edodes mycelium covers the surface of the fresh corn straw slice; (2) secondary inoculation field planting corn stalk: removing the mushroom mycelium inoculation block in the step (1), vertically inserting a fresh corn stalk slice, enabling the bottom of the fresh corn stalk slice to be in contact with the tiled fresh corn stalk slice covered with the mushroom mycelium, controlling the humidity of the culture environment to be more than 80% in a breathable closed sterile environment, culturing at room temperature in a dark way, and continuously performing dark culture or illumination culture until the mushroom mycelium forms fruiting bodies when the vertically inserted fresh corn stalk slice is full of mycelium. The mushroom culture period is shortened to 25-35 days by adopting the method.
Description
Technical Field
The invention belongs to the field of edible fungus breeding, and particularly relates to a mushroom fruiting body model cultivation method.
Background
Lentinus edodes (Lentinus edodes) belongs to the genus Lentinus (Lentinus) of the order Agaricales (Agaricolas) of the order Agaricales (Agaricola) of the order Agaricales (Basidiomyceta) of the fungus kingdom. The lentinus edodes has rich nutrition, delicious taste and unique flavor, is rich in essential amino acids and dietary fibers of human bodies, and the lentinan has antitumor activity and is one of the important edible fungi in the world. The artificial cultivation of Lentinus edodes is mainly concentrated in China, japan and Korea, wherein China contributes more than 80% of the world Lentinus edodes yield. The China is the earliest country which starts artificial cultivation in the world, and has been 800 years old, from log cultivation to material generation cultivation, to the present highly-automated factory cultivation, and the cultivation mode of the cultivation is continuously evolved.
Although the artificial cultivation technology of the lentinus edodes is mature, related basic research is also carried out in succession, and research including genome, transcriptome sequencing, metabolome, functional gene verification and the like is combined with the emerging biological technical means, so that many scientific problems needing to be researched and explored still remain, how to explore fruiting body development, shorten cultivation period, improve cultivation material utilization rate or replace wood chips by crop straws and the like.
In accordance with the search result, the problems related to the formation of fruiting bodies, the utilization of novel nutrients (media) and conditions and the like of the above-mentioned patents, such as a cultivation method of pleurotus eryngii fruiting bodies by controlling the quantity of pleurotus eryngii fruiting bodies through illumination, a method for inducing the formation of coral fungus fruiting bodies, a method for artificially cultivating wild agaricus bisporus fruiting bodies, a novel fruiting method for mushrooms, a cultivation method for pleurotus eryngii, a method for inducing the formation of oyster mushroom primordium, a method for recycling agricultural wastes, a cultivation medium for flammulina velutipes, a cultivation method for cultivating corn straw flammulina velutipes, a cultivation method for cultivating mushrooms in a crop rotation mode, a cultivation method for cultivating mushrooms with corn straw as a medium, a cultivation method for cultivating mushrooms with pure raw materials, a cultivation method for mushrooms, a cultivation method for edible mushrooms, and the like, are mainly concentrated in the cultivation process.
The period from inoculation and spawning to color conversion and fruiting of the mushrooms is long, and generally requires 70-110 days. The method is realized by combining cultivation production in the breeding process of the mushroom variety and the research of the development of fruiting bodies, and has higher time cost. At present, few reports exist on how to culture mushrooms and rapidly fruiting under laboratory conditions.
Disclosure of Invention
Aiming at the defects or improvement demands of the prior art, the invention provides a mushroom fruiting body model cultivation method, which aims to shorten the cultivation period from 70-110 days to 25-35 days by inoculating and planting fresh corn straw slices twice for mushroom fruiting body model cultivation, thereby solving the technical problem of long cultivation period of mushroom fruiting bodies under the existing experimental conditions.
In order to achieve the above object, according to one aspect of the present invention, there is provided a cultivation method of a fruiting body model of Lentinus edodes, comprising the steps of:
(1) One-time inoculation field planting fresh corn stalk
Inoculating a lentinus edodes mycelium inoculation block to the surface of a tiled fresh corn straw slice, culturing in a breathable closed sterile environment, controlling the humidity of the culture environment to be more than 80%, and culturing at room temperature in a dark place until the lentinus edodes mycelium covers the surface of the fresh corn straw slice;
(2) Secondary inoculation field planting fresh corn stalk
Removing the inoculation block of the lentinus edodes mycelium in the step (1), vertically inserting fresh corn stalk slices, enabling the bottoms of the fresh corn stalk slices to be in contact with the tiled fresh corn stalk slices covered with the lentinus edodes mycelium, controlling the humidity of a culture environment to be more than 80% in a breathable airtight sterile environment, culturing the fresh corn stalk slices at room temperature in a dark place, and continuously culturing the fresh corn stalk slices until the lentinus edodes mycelium grows into fruiting bodies.
Preferably, in the cultivation method of the mushroom fruiting body model, the fresh corn stalk slices are slices which do not contain knots and have the thickness of 1-2 mm.
Preferably, in the cultivation method of the mushroom fruiting body model, in the step (1), the tiled fresh corn stalk slices have the length of not more than 6cm; and (2) vertically inserting fresh corn stalk slices, wherein the length of each fresh corn stalk slice is 8-10cm.
Preferably, in the cultivation method of the fruiting body model of Lentinus Edodes, the room temperature light-shielding cultivation in the step (1) is 5-10 days.
Preferably, in the cultivation method of the fruiting body model of Lentinus Edodes, the room temperature light-shielding cultivation in the step (2) is 8-12 days.
Preferably, in the cultivation method of the mushroom fruiting body model, the humidity of the cultivation environment is 80-90%.
Preferably, in the cultivation method of the mushroom fruiting body model, the humidity of the controlled cultivation environment is controlled by adopting water-absorbing vermiculite, and the thickness of the vermiculite layer is 15-20mm.
Preferably, in the cultivation method of the mushroom fruiting body model, the diameter of the water-absorbing vermiculite is 2-4mm.
Preferably, in the cultivation method of the fruiting body model of the lentinus edodes, when the lentinus edodes mycelium grows fully with the vertically inserted fresh corn stalk slices, the cultivation is continued in a dark place or in an illumination place until the lentinus edodes mycelium forms fruiting bodies; preferably, the germinated fruiting body is cultured under natural illumination or artificial illumination until the fruiting body color is changed into Lentinus Edodes color during production.
Preferably, in the cultivation method of the fruiting body model of the lentinus edodes, the inoculated blocks of the lentinus edodes mycelia are bacterial cakes with the diameter of 5-10mm or bacterial blocks with the corresponding size; the culture medium for inoculating the lentinus edodes mycelium comprises 20g of malt extract, 20g of glucose, 1g of peptone, 1g of yeast extract, 20g of agar and the balance of water per liter.
In general, compared with the prior art, the technical scheme of the invention is characterized in that the fresh corn straw slice culture medium is adopted to culture the mushrooms, experimental results show that the mushrooms are cultivated for 25-35 days, the mushrooms are cultivated by adopting oak and straw culture medium for the same time without fruiting, compared with the common wood substrate cultivation, the mushroom cultivation period is generally 70-110 days, the fresh corn straw slice culture is adopted, the mushroom fruiting time is 25-35 days, the fresh corn straw shows remarkable advantages in the fruiting speed of mushroom fruiting bodies, and the fresh corn straw slice is adopted as the culture medium, so that the mushroom fruiting time can be remarkably shortened, the mushroom maturation period can be shortened, the mushroom fruiting body model can be cultivated, and the time cost of related mushroom researches is greatly reduced.
The fresh corn stalk slices can obviously shorten mushroom fruiting time, which is probably due to the fact that free sugar, micromolecules and moisture content in the fresh corn stalk are different from common wood base materials, the fresh corn stalk slices can be used as cellulose base materials, nutrition and moisture can be provided, the fresh corn stalk slices are good in toughness, different from other existing stalk material tiling filling states, the vertically inserted fresh corn stalk can effectively support the germination process of mycelium forming fruiting bodies, mushroom fruiting is facilitated, and the mushroom fruiting time can be obviously shortened.
Drawings
FIG. 1 shows the results of activated culture of mycelium of Lentinus edodes strain W1 on MYG solid medium;
FIG. 2 is a tissue culture flask and vermiculite;
FIG. 3 is a view of fresh cornstalks of different treatments, wherein A is cleaned fresh cornstalks, B is fresh cornstalk flakes, and C is sterilized cornstalk flakes;
FIG. 4 shows the first and second inoculation of mycelium blocks of Lentinus edodes strain W1 to fresh corn stover, wherein A is before the first inoculation of fresh corn stover; b is after first inoculation and planting of fresh corn stalks; c is before the second inoculation of the field planting fresh corn stalks; d is after the second inoculation field planting of fresh corn stalks;
FIG. 5 is a graph showing the result of fruiting fresh corn stalk culture; in the figure, A is dark culture, and B is illumination culture;
FIG. 6 culture substrate oak controls before and after sterilization; in the figure, A is oak before sterilization, and B is oak after sterilization;
FIG. 7 shows that the mycelium blocks of the Lentinus edodes strain W1 are inoculated with oak for the first time and the second time, wherein A is before the oak is inoculated and planted for the first time, B is after the oak is inoculated and planted for the second time, C is before the oak is inoculated and planted for the second time, and D is after the oak is inoculated and planted for the second time;
FIG. 8 is a graph showing a control of the culture substrate straw before and after sterilization, wherein A is the straw before sterilization and B is the straw after sterilization;
FIG. 9 shows that the mycelium pellet of Lentinus edodes strain W1 is inoculated with the planted rice straw for the first time and the second time, wherein A is before the first inoculation of the planted rice straw, B is after the second inoculation of the planted rice straw, C is before the second inoculation of the planted rice straw, and D is after the second inoculation of the planted rice straw;
FIG. 10 shows the results of oak and straw cultures for the same time without fruiting.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Lentinus edodes is an important lignocellulose degrading fungus, and the sequenced genome of Lentinus edodes contains rich lignocellulose degrading enzyme genes, so that Lentinus edodes has unique enzymolysis adaptability to wood lignocellulose.
The existing mushroom culture generally adopts wood dust, crop straws and the like such as rice straws. We have found that Lentinus edodes has wide applicability to non-woody plant cell wall polysaccharides, such as corn stover; more importantly, the culture medium is found to have a remarkable influence on the fruiting time of the mushroom mycelia, experiments prove that oak, straw and fresh corn stalks have a remarkable influence on the fruiting period of the mushrooms, and experimental results show that the fruiting time of the mushrooms is shortened to 25-35 days by adopting the fresh corn stalk slice culture medium for culture, the fruiting period of the mushrooms can be remarkably shortened, the fruiting body of the mushrooms can be used for culturing fruiting body models of the mushrooms, further research on other aspects of the mushrooms in later period is facilitated, the experimental period is greatly shortened, and the experimental time cost is saved.
The invention provides a mushroom fruiting body model cultivation method, which comprises the following steps:
(1) One-time inoculation field planting fresh corn stalk slice
And (3) inoculating the activated mushroom mycelia in the solid culture medium to the surface of a tiled fresh corn straw slice by taking an inoculating block with a proper size as shown in fig. 1, culturing in a breathable closed sterile environment, controlling the humidity of the culture environment to be more than 80%, and culturing at room temperature and in a dark place for 5-10 days until the mushroom mycelia cover the surface of the fresh corn straw slice.
The fresh corn stalk slice is preferably a slice which does not contain knots and has the thickness of 1-2mm, and is prepared by the following method:
in the corn harvesting season, the corn stalks without knots after harvesting are cleaned up, cut into slices with the thickness of 1-2mm longitudinally, and sterilized at high temperature and high pressure to obtain the corn stalk harvester shown in figure 3.
The inoculation block with the proper size is a fungus block with the size of 5-10mm, and is obtained according to the following method:
inoculating Lentinus edodes mycelium into a solid MYG culture medium, sealing, standing at room temperature, and culturing to obtain a solid culture medium covered by mycelium as an inoculating block; the MYG culture medium comprises 20g of malt extract, 20g of glucose, 1g of peptone, 1g of yeast extract, 20g of agar and the balance of water.
(2) Fresh corn stalk slice for secondary inoculation and field planting
Removing the lentinus edodes mycelium inoculation block in the step (1), vertically inserting fresh corn straw slices, enabling the bottoms of the fresh corn straw slices to be in contact with the tiled fresh corn straw slices covered with lentinus edodes mycelium, controlling the humidity of a culture environment to be more than 80% in a breathable airtight sterile environment, and culturing the fresh corn straw slices in a light-proof mode at room temperature, and continuously culturing the fresh corn straw slices in a light-proof mode or culturing the fresh corn straw slices in an illumination mode until the lentinus edodes mycelium forms fruiting bodies after the vertically inserted fresh corn straw slices are full of lentinus edodes mycelium; preferably, the germinated fruiting body is cultured under natural illumination or artificial illumination until the fruiting body color is changed into Lentinus Edodes color during production.
The fresh corn stalk slices which are tiled in the step (1) are preferably slices of the fresh corn stalk with the length not exceeding 6cm, and the tiled fresh corn stalk slices can be tiled on the vermiculite layers at the bottom of the tissue culture bottle.
And (2) vertically placing the fresh corn stalk slices, preferably the fresh corn stalk slices with the length of 8-10cm.
The breathable closed sterile environment in the step (2) is preferably formed by using sterilized tissue culture bottles.
The humidity of the culture environment is preferably controlled to 80-90%.
The culture environment humidity is controlled, and the water absorption vermiculite is adopted to control the environment humidity; preferably the vermiculite layer has a thickness of 15-20mm, more preferably 2-4mm diameter, as shown in figure 2.
The following are examples:
example 1 cultivation of Lentinus Edodes fruiting body with fresh corn straw slices
(1) One-time inoculation field planting corn stalk: inoculating the lentinus edodes mycelium inoculation block activated by the solid culture medium to the surface of a tiled fresh corn straw slice as shown in A in fig. 4, controlling the humidity to be above 80% in a breathable airtight sterile environment, and culturing for 5-10 days at room temperature in a dark place until the lentinus edodes mycelium covers the surface of the fresh corn straw slice as shown in B in fig. 4; the method comprises the following specific steps:
(1-1) activation of Lentinus Edodes Strain Wuxiang No. 1 (W1):
inoculating mycelium of Lentinus Edodes strain W1 preserved at low temperature on solid MYG in ultra-clean workbench, sealing, standing at 25deg.C for 7 days, and standing until mycelium is fully distributed on the solid MYG culture medium.
Solid malt extract culture Medium (MYG) formula: malt extract (Malt extract) 20g, glucose (Glucose) 20g, peptone (Peptone) 1g, yeast extract (Yeast extract) 1g, agar (Agar) 20g, ddH is added 2 O to 1L.
(1-2) preparation of an inoculation environment:
preparing corn stalks: the harvested cornstalks are cleaned up and cut longitudinally into slices (without knots) of 1-2mm thickness, and sterilized at high temperature and high pressure for later use, as shown in fig. 3. In the test, the corn stalks are from the agricultural university test field in China.
Preparing a tissue culture bottle: a certain number of clean transparent glass tissue culture bottles are prepared, and the specifications are as follows: the high temperature resistant sealing cover with the ventilation holes has the capacity of about 350ml, the height of 108mm and the diameter of 75 mm. According to the number of the tissue culture bottles, a proper amount of coarse vermiculite (with different diameters of 2-4 mm) is correspondingly prepared, a proper amount of distilled water is added, the water adding amount is based on that the vermiculite absorbs water but does not exude, and finally, the vermiculite is subpackaged into each tissue culture bottle, the thickness is about 15-20mm, a cover is covered for sealing, and high-temperature high-pressure sterilization (121 ℃ for 30 min) is carried out for later use. Preparing vermiculite and distilled water of 500-1000ml, and sterilizing together for later use.
(1-3) inoculation procedure:
placing the sterilized tissue culture bottle, cornstalks and sterilized distilled water into an ultra-clean workbench, turning on an ultraviolet lamp for surface sterilization (30 min), turning off the ultraviolet lamp, and turning on a fan and an illuminating lamp to start the inoculation culture step of the first step. And the subsequent inoculation operation process needs a sterile environment, so that the pollution of mixed bacteria is avoided.
Firstly, the sterilized cornstalk slices are cut into small sections, the length is not more than 60mm, and the sections are horizontally placed on the surface of vermiculite. Then inoculating activated Lentinus Edodes strain W1 hypha together with culture medium into blocks with size of 5-10mm, placing on fresh corn stalk slice, and sealing with cover. Then, the mixture is subjected to standing and dark culture at 25 ℃ for about one week (model of incubator: ZSX1500GS, wuhan Ruihua instrument and equipment Limited liability company), and mycelia are grown on fresh corn stalk slices.
(2) Secondary inoculation field planting corn stalk: removing the lentinus edodes mycelium inoculation block, vertically inserting fresh corn stalk slices, enabling the bottoms of the fresh corn stalk slices to be in contact with the tiled fresh corn stalk slices covered with lentinus edodes mycelium, and culturing the fresh corn stalk slices for 8 to 12 days at room temperature and in a light-proof mode until the lentinus edodes mycelium forms fruiting bodies, wherein the humidity is controlled to be more than 80% under a breathable airtight sterile environment, as shown in a graph (C) in FIG. 4; the specific operation is as follows:
removing the mushroom hypha inoculation block in a sterile super clean bench, simultaneously vertically placing another fresh corn straw slice, contacting the fresh corn straw slice full of hypha at the bottom, and leaning the top against the wall of the tissue culture bottle; then adding a proper amount of sterile distilled water slowly along the top of the vertically placed fresh corn stalk slice, and sealing the cover if the distilled water does not overflow the surface of vermiculite. Finally, standing and dark culturing (model of incubator: ZSX1500GS, wuhan Ruihua instrument and equipment Limited liability company) at 25 ℃ for 10 days, wherein the mushroom fruiting body is observed to grow out from the top of the fresh corn stalk slice horizontally placed at the bottom or along the front or back of the vertically placed fresh corn stalk slice.
(3) And (3) light culture: natural illumination and culturing at 25deg.C until the color of the germinated Lentinus edodes fruiting body is changed to Lentinus edodes color during production, as shown in figure 5B.
Comparative example 1 cultivation of Lentinus Edodes fruiting body with oak culture medium
The fresh corn stalk slices in example 1 were replaced with oak slices, and other operations and conditions were the same as in example 1, the oak slices were sterilized before and after sterilization, and the results were shown in fig. 7, wherein a in fig. 7 is before the first inoculation and planting, B is after the first inoculation and planting, C is before the second inoculation and planting, and D is after the second inoculation and planting. After the same cultivation time and conditions as in example 1, no fruiting was seen in oak cultivation, and even autophagy started in the bottom hypha, as shown in fig. 10 a.
Comparative example 2 cultivation of Lentinus edodes fruiting body with straw culture medium
The fresh corn stalk slices in example 1 were replaced with straw, and other operations and conditions were the same as in example 1, the straw was sterilized before and after the sterilization, and the results were shown in fig. 9, wherein a in fig. 9 is before the first inoculation and planting, B is after the first inoculation and planting, C is before the second inoculation and planting, and D is after the second inoculation and planting. After the same cultivation time and conditions as in example 1, no fruiting was observed in the straw cultivation, and the mycelia were rare and the viability was low, as shown in FIG. 10B.
As is clear from example 1 and comparative examples 1-2, under the laboratory conditions of more strictly controlling the environmental humidity and atmosphere, the fresh corn stalk slice culture medium is adopted to cultivate the mushrooms, the fruiting bodies of the mushrooms can be cultivated rapidly only for 25-35 days from inoculation to fruiting, the oak slice and straw culture medium are adopted to cultivate the mushrooms, the mushrooms are not fruiting in 25-35 days of cultivation, and the mycelia at the bottom of the oak culture medium begin to autophagy, so that the mycelia of the straw culture medium are rare and have low activity. Compared with the mushroom fruiting body maturation period of 70-110 days under the current experimental conditions, the fresh corn stalks obviously shorten the mushroom fruiting body maturation period, which is probably due to the difference of free sugar, small molecular components and water content in the fresh corn stalks, rather than being used as a cellulose base material, and the fresh corn stalks have good toughness, are different from the tiling filling state of other stalk materials, and the vertically inserted fresh corn stalk slices can effectively support the sprouting process of mycelium to form fruiting bodies, thereby being beneficial to fruiting.
The mushroom fruiting body model cultivation method provided by the invention greatly shortens the mushroom fruiting body cultivation period, greatly saves the time cost of mushroom fruiting body cultivation, remarkably improves the mushroom breeding efficiency, and provides a good cultivation mode for researching fruiting body development.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the invention and is not intended to limit the invention, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (8)
1. The mushroom fruiting body model cultivation method is characterized by comprising the following steps of:
(1) One-time inoculation field planting fresh corn stalk
Inoculating a lentinus edodes mycelium inoculation block to the surface of a tiled fresh corn straw slice, culturing in a breathable closed sterile environment, controlling the humidity of the culture environment to be more than 80%, and culturing at room temperature in a dark place until the lentinus edodes mycelium covers the surface of the fresh corn straw slice;
(2) Secondary inoculation field planting fresh corn stalk
Removing the lentinus edodes mycelium inoculation block in the step (1), vertically inserting a fresh corn stalk slice, enabling the bottom of the fresh corn stalk slice to be in contact with the tiled fresh corn stalk slice covered with lentinus edodes mycelium, controlling the humidity of a culture environment to be more than 80% in a breathable closed sterile environment, culturing at room temperature in a dark place, and continuing culturing until lentinus edodes mycelium forms fruiting bodies after the lentinus edodes mycelium grows full of the vertically inserted fresh corn stalk slice;
the fresh corn stalk slices are slices which do not contain knots and have the thickness of 1-2 mm;
the length of the tiled fresh corn stalk slices in the step (1) is not more than 6cm; and (2) vertically inserting fresh corn stalk slices, wherein the length of each fresh corn stalk slice is 8-10cm.
2. The method for cultivating a fruiting body model of Lentinus Edodes according to claim 1, wherein the culturing in the step (1) is carried out at room temperature for 5-10 days.
3. The method for cultivating a fruiting body model of Lentinus Edodes according to claim 2, wherein the culturing in the step (2) is carried out at room temperature for 8-12 days.
4. A method of cultivating a fruiting body model of Lentinus Edodes according to claim 1, wherein the humidity of the cultivation environment is 80-90%.
5. A method for cultivating a fruiting body model of Lentinus Edodes according to claim 4, wherein the humidity of the cultivation environment is controlled by water-absorbing vermiculite, and the thickness of the vermiculite layer is 15-20mm.
6. A method of cultivating a fruiting body model of Lentinus Edodes according to claim 5, wherein the diameter of the water absorbing vermiculite is 2-4mm.
7. The cultivation method of fruiting body model of Lentinus Edodes of claim 1, wherein when Lentinus Edodes mycelium grows on fresh corn stalk slices inserted vertically, continuing light-proof culture or illumination culture until Lentinus Edodes mycelium forms fruiting body; culturing the germinated fruiting body under natural or artificial illumination to convert fruiting body color into Lentinus Edodes color.
8. A method for cultivating a fruiting body model of Lentinus Edodes according to any one of claims 1 to 7, wherein the inoculating block of Lentinus Edodes mycelium is a bacterial cake with diameter of 5-10mm or bacterial block with corresponding size, the inoculating culture medium of Lentinus Edodes mycelium contains malt extract 20g, glucose 20g, peptone 1g, yeast extract 1g, agar 20g, and water for the rest.
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