CN112341268A - Culture solution for cultivating mushrooms and preparation method thereof - Google Patents

Culture solution for cultivating mushrooms and preparation method thereof Download PDF

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Publication number
CN112341268A
CN112341268A CN202011178493.XA CN202011178493A CN112341268A CN 112341268 A CN112341268 A CN 112341268A CN 202011178493 A CN202011178493 A CN 202011178493A CN 112341268 A CN112341268 A CN 112341268A
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culture solution
auxin
vitamin
carbon source
nitrogen source
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吴迪
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a culture solution for cultivating mushrooms and a preparation method thereof, wherein the culture solution comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1 percent of water for the rest; the carbon source is glucose, sucrose and other saccharides, and the nitrogen source is one of urea, amino acid, peptone and protein. According to the invention, by arranging the mutual matching of the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin, the advantage of improving the nutrition degree of the culture solution is achieved, and the problems of low activity and weak vitality of the mushroom caused by poor nutrition of the culture solution when the mushroom is cultured by the culture solution due to incomplete nutrition of the existing culture solution are solved, so that the nutrition degree of the culture solution can be comprehensively improved, the activity of the mushroom is improved, the vitality of the mushroom is improved, and the yield of the mushroom is increased when the mushroom is cultured by the culture solution.

Description

Culture solution for cultivating mushrooms and preparation method thereof
Technical Field
The invention relates to the technical field of mushroom cultivation, in particular to a culture solution for mushroom cultivation and a preparation method thereof.
Background
The mushroom is called agaricus bisporus, also called white mushroom and agaricus bisporus, belongs to the order of agaricus, the family of agaricus, the genus of agaricus, is an edible mushroom variety with wide artificial cultivation, high yield and large consumption in the world, is cultivated in many countries, and the total yield of China accounts for the second place in the world. In recent years, with the rapid development of the edible mushroom industry, the yield of agaricus bisporus is also increasing year by year, and the agaricus bisporus becomes a post industry for increasing the income of farmers in many areas. Along with the improvement of the living standard of people, the annual consumption demand of mushrooms is continuously increased, the industrialized cultivation of the agaricus bisporus is also realized, the edible mushrooms are various, the cultivation is less, the development capacity is high, agricultural and sideline product leftovers such as wheat straws, wood chips and corn stalks and the like which are used as cultivation raw materials are available all over the country and are suitable for the growth of various edible mushrooms, the popularization and the development are convenient, the mushrooms are various, the flammulina velutipes and the oyster mushrooms are different, the encyclopedia of each mushroom can be consulted specifically, the flammulina velutipes is taken as an example, the flammulina velutipes cultivation method has two types of clinker cultivation and raw material cultivation, the cultivation means that the agaricus velutipes are promoted to occur, grow and propagate under appropriate conditions, and the flammulina velutipes are promoted to grow and propagate according to certain purposes for a long time of education and training.
Fungi, a large group of heterotrophic organisms which do not contain chlorophyll and can not carry out photosynthesis, without differentiation of roots, stems and leaves, without photosynthetic pigments such as chlorophyll (except for a very small number of photosynthetic bacteria), and can not carry out photosynthesis, saprophytic life or parasitic life, i.e., heterotrophic life, most of reproductive organs are in a single cell structure, zygotes do not develop into embryos, the fungi are a huge family which is ubiquitous, and at present, the known fungi have about 12 ten thousand species, and can be divided into three types of organisms which have no relationship with each other, namely phylum bacteroides, phylum myxomycota and phylum mycomycota, wherein the myxomycota is an organism between animals and fungi, and is a naked, cell wall-free and multinucleated protoplasm body which is called a proteosome (similar to a proteosome) in a vegetative stage, but can produce spores with a cellulose cell wall and has characteristics of fungi, and the fungi have simple structure without roots, leaves, and leaves, Organs such as stems and leaves generally do not contain pigments such as chlorophyll, and most of them live in heterotrophic life.
When the mushrooms are cultivated, a culture solution is needed, and the existing culture solution has the following defects: the existing culture solution is not comprehensive in nutrition, so that when the culture solution is used for culturing the mushroom, the vitality of the mushroom is low and the vitality of the mushroom is not strong due to poor nutrition of the culture solution, the yield of the mushroom is reduced, and the use requirement of a user cannot be met.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the culture solution for cultivating the mushrooms and the preparation method thereof, which have the advantage of improving the nutrition of the culture solution, and solve the problems of low vitality and weak vitality of the mushrooms caused by poor nutrition of the culture solution when the mushrooms are cultivated by the culture solution due to incomplete nutrition of the existing culture solution.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Preferably, the carbon source is a saccharide such as glucose and sucrose, and the nitrogen source is one of urea, amino acid, peptone, and protein.
Preferably, the macro-elements may be one of monopotassium phosphate, dipotassium phosphate, magnesium sulfate, gypsum powder and superphosphate, and the micro-elements may be one of iron, copper, zinc, manganese, boron, cobalt and molybdenum.
Preferably, the vitamin may be one of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin H and vitamin PP, and the auxin may be one of nucleoside, nucleotide, cyclic adenosine monophosphate, naphthylacetic acid, gibberellin, indoleacetic acid and indolebutyric acid.
Preferably, the components of the culture solution in percentage are as follows: carbon source: 5% and nitrogen source: 1%, macroelements: 0.1%, trace elements: 0.001%, vitamins: 0.1%, auxin: 0.05% and the balance of water.
Preferably, the components of the culture solution in percentage are as follows: carbon source: 8% and nitrogen source: 3% and macroelements: 0.3%, trace elements: 0.003%, vitamins: 0.3%, auxin: 0.08 percent and the balance of water.
Preferably, the components of the culture solution in percentage are as follows: carbon source: 10% and nitrogen source: 5%, macroelements: 0.5%, trace elements: 0.005%, vitamins: 0.5%, auxin: 0.1 percent and the balance of water.
A preparation method of a culture solution for cultivating mushrooms comprises the following steps:
A. raw material purchasing: the purchasing personnel calculates the quantity of raw materials to be purchased according to the order and purchases the raw materials according to the quantity, and the raw materials are required to be fresh, clean and mildew-free no matter what raw materials are selected;
B. weighing: weighing the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin according to the calculated amount, weighing strictly according to the calculated proportion, then calculating the weight of the water, and then weighing the water;
C. primary sterilization: then putting the raw materials into a sterilization device, sterilizing for 40-50 minutes at 110-115 ℃ and 0.1-0.15 MPa, and taking out after sterilization;
D. mixing: adding the weighed water obtained in the step B into a mixing tank, respectively adding the weighed carbon source, nitrogen source, major elements, trace elements, vitamins and auxin obtained in the step B into the mixing tank, starting the mixing tank to mix the raw materials, wherein the mixing temperature is 40-50 ℃, and the mixing time is 50-60 minutes;
E. secondary sterilization: d, barreling the culture solution uniformly stirred in the step D, placing the culture solution in a sterilization device, sterilizing the culture solution at the temperature of between 110 and 115 ℃ for 20 to 30 minutes, and taking out the culture solution after the sterilization is finished;
F. filling: and (4) inputting the mixed culture solution into a sealed storage tank for storage, thereby obtaining the required culture solution.
G. And (3) storage: then the filled culture solution is put in a warehouse, the temperature is 15-25 ℃ when the culture solution is stored, and the direct irradiation of sunlight is avoided.
(III) advantageous effects
Compared with the prior art, the invention provides the culture solution for cultivating the mushrooms and the preparation method thereof, and the culture solution has the following beneficial effects:
according to the invention, by arranging the mutual matching of the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin, the advantage of improving the nutrition degree of the culture solution is achieved, and the problems of low activity and weak vitality of the mushroom caused by poor nutrition of the culture solution when the mushroom is cultured by the culture solution due to incomplete nutrition of the existing culture solution are solved, so that the nutrition degree of the culture solution can be comprehensively improved, the activity of the mushroom is improved, the vitality of the mushroom is improved, the yield of the mushroom is increased, and the use requirements of users can be met.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
All the components of the present invention are standard components commonly used or known to those skilled in the art, and the structure and principle thereof can be known to those skilled in the art through technical manuals or through routine experimentation.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
According to the invention, by arranging the mutual matching of the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin, the advantage of improving the nutrition degree of the culture solution is achieved, and the problems of low activity and weak vitality of the mushroom caused by poor nutrition of the culture solution when the mushroom is cultured by the culture solution due to incomplete nutrition of the existing culture solution are solved, so that the nutrition degree of the culture solution can be comprehensively improved, the activity of the mushroom is improved, the vitality of the mushroom is improved, the yield of the mushroom is increased, and the use requirements of users can be met.
The first embodiment is as follows:
a culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example two:
in the first embodiment, the following steps are added:
the carbon source is glucose, sucrose and other saccharides, and the nitrogen source is one of urea, amino acid, peptone and protein.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example three:
in the second embodiment, the following steps are added:
the macroelement can be one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, gypsum powder and calcium superphosphate, and the microelement can be one of iron, copper, zinc, manganese, boron, cobalt and molybdenum.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example four:
in the third embodiment, the following steps are added:
the vitamin can be one of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin H and vitamin PP, and the auxin can be one of nucleoside, nucleotide, cyclic adenosine monophosphate, naphthylacetic acid, gibberellin, indoleacetic acid and indolebutyric acid.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example five:
in the fourth example, the following steps were added:
the culture solution comprises the following components in percentage by weight: carbon source: 5% and nitrogen source: 1%, macroelements: 0.1%, trace elements: 0.001%, vitamins: 0.1%, auxin: 0.05% and the balance of water.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example six:
in the fifth example, the following steps were added:
the culture solution comprises the following components in percentage by weight: carbon source: 8% and nitrogen source: 3% and macroelements: 0.3%, trace elements: 0.003%, vitamins: 0.3%, auxin: 0.08 percent and the balance of water.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
Example seven:
in example six, the following steps were added:
the culture solution comprises the following components in percentage by weight: carbon source: 10% and nitrogen source: 5%, macroelements: 0.5%, trace elements: 0.005%, vitamins: 0.5%, auxin: 0.1 percent and the balance of water.
A culture solution for cultivating mushrooms comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
A preparation method of a culture solution for cultivating mushrooms comprises the following steps:
A. raw material purchasing: the purchasing personnel calculates the quantity of raw materials to be purchased according to the order and purchases the raw materials according to the quantity, and the raw materials are required to be fresh, clean and mildew-free no matter what raw materials are selected;
B. weighing: weighing the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin according to the calculated amount, weighing strictly according to the calculated proportion, then calculating the weight of the water, and then weighing the water;
C. primary sterilization: then putting the raw materials into a sterilization device, sterilizing for 40-50 minutes at 110-115 ℃ and 0.1-0.15 MPa, and taking out after sterilization;
D. mixing: adding the weighed water obtained in the step B into a mixing tank, respectively adding the weighed carbon source, nitrogen source, major elements, trace elements, vitamins and auxin obtained in the step B into the mixing tank, starting the mixing tank to mix the raw materials, wherein the mixing temperature is 40-50 ℃, and the mixing time is 50-60 minutes;
E. secondary sterilization: d, barreling the culture solution uniformly stirred in the step D, placing the culture solution in a sterilization device, sterilizing the culture solution at the temperature of between 110 and 115 ℃ for 20 to 30 minutes, and taking out the culture solution after the sterilization is finished;
F. filling: and (4) inputting the mixed culture solution into a sealed storage tank for storage, thereby obtaining the required culture solution.
G. And (3) storage: then the filled culture solution is put in a warehouse, the temperature is 15-25 ℃ when the culture solution is stored, and the direct irradiation of sunlight is avoided.
According to the invention, by arranging the mutual matching of the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin, the advantage of improving the nutrition degree of the culture solution is achieved, and the problems of low activity and weak vitality of the mushroom caused by poor nutrition of the culture solution when the mushroom is cultured by the culture solution due to incomplete nutrition of the existing culture solution are solved, so that the nutrition degree of the culture solution can be comprehensively improved, the activity of the mushroom is improved, the vitality of the mushroom is improved, the yield of the mushroom is increased, and the use requirements of users can be met.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides a fungus mushroom is cultivated and uses culture solution which characterized in that: the culture solution comprises the following components in percentage by weight: carbon source: 5-10%, nitrogen source: 1-5%, macroelements: 0.1-0.5%, trace elements: 0.001-0.005%, vitamin: 0.1-0.5%, auxin: 0.05-0.1% and the balance of water.
2. The culture solution for cultivating mushrooms according to claim 1, wherein: the carbon source is glucose, sucrose and other saccharides, and the nitrogen source is one of urea, amino acid, peptone and protein.
3. The culture solution for cultivating mushrooms according to claim 1, wherein: the macroelement can be one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, gypsum powder and calcium superphosphate, and the microelement can be one of iron, copper, zinc, manganese, boron, cobalt and molybdenum.
4. The culture solution for cultivating mushrooms according to claim 1, wherein: the vitamin may be one of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin H and vitamin PP, and the auxin may be one of nucleoside, nucleotide, cyclic adenosine monophosphate, naphthylacetic acid, gibberellin, indoleacetic acid and indolebutyric acid.
5. The culture solution for cultivating mushrooms according to claim 1, wherein: the culture solution comprises the following components in percentage by weight: carbon source: 5% and nitrogen source: 1%, macroelements: 0.1%, trace elements: 0.001%, vitamins: 0.1%, auxin: 0.05% and the balance of water.
6. The culture solution for cultivating mushrooms according to claim 1, wherein: the culture solution comprises the following components in percentage by weight: carbon source: 8% and nitrogen source: 3% and macroelements: 0.3%, trace elements: 0.003%, vitamins: 0.3%, auxin: 0.08 percent and the balance of water.
7. The culture solution for cultivating mushrooms according to claim 1, wherein: the culture solution comprises the following components in percentage by weight: carbon source: 10% and nitrogen source: 5%, macroelements: 0.5%, trace elements: 0.005%, vitamins: 0.5%, auxin: 0.1 percent and the balance of water.
8. A preparation method of a culture solution for cultivating mushrooms is characterized by comprising the following steps: the preparation method comprises the following steps:
A. raw material purchasing: the purchasing personnel calculates the quantity of raw materials to be purchased according to the order and purchases the raw materials according to the quantity, and the raw materials are required to be fresh, clean and mildew-free no matter what raw materials are selected;
B. weighing: weighing the carbon source, the nitrogen source, the major elements, the trace elements, the vitamins and the auxin according to the calculated amount, weighing strictly according to the calculated proportion, then calculating the weight of the water, and then weighing the water;
C. primary sterilization: then putting the raw materials into a sterilization device, sterilizing for 40-50 minutes at 110-115 ℃ and 0.1-0.15 MPa, and taking out after sterilization;
D. mixing: adding the weighed water obtained in the step B into a mixing tank, respectively adding the weighed carbon source, nitrogen source, major elements, trace elements, vitamins and auxin obtained in the step B into the mixing tank, starting the mixing tank to mix the raw materials, wherein the mixing temperature is 40-50 ℃, and the mixing time is 50-60 minutes;
E. secondary sterilization: d, barreling the culture solution uniformly stirred in the step D, placing the culture solution in a sterilization device, sterilizing the culture solution at the temperature of between 110 and 115 ℃ for 20 to 30 minutes, and taking out the culture solution after the sterilization is finished;
F. filling: inputting the mixed culture solution into a sealed storage tank for storage, thereby obtaining the required culture solution;
G. and (3) storage: then the filled culture solution is put in a warehouse, the temperature is 15-25 ℃ when the culture solution is stored, and the direct irradiation of sunlight is avoided.
CN202011178493.XA 2020-10-29 2020-10-29 Culture solution for cultivating mushrooms and preparation method thereof Pending CN112341268A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1050484A (en) * 1989-09-29 1991-04-10 赵清华 The preparation of edible fungus production increasing agent and using method
CN102090264A (en) * 2009-12-10 2011-06-15 上海市农业科学院 Method for liquid cultivation of hypsizygus marmoreus
CN103168613A (en) * 2011-12-23 2013-06-26 何寒 Fast manufacture method for liquid strain
CN104261942A (en) * 2014-09-28 2015-01-07 永和县旺达食用菌有限责任公司 Pleurotus nebrodensis liquid strain culture medium and propagation method of strain
CN104710206A (en) * 2013-07-12 2015-06-17 鲁东大学 Preparation method for volvariella volvacea liquid strain
CN109006164A (en) * 2018-08-10 2018-12-18 安徽泓森生物科技有限公司 Agricus blazei liquid culture method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1050484A (en) * 1989-09-29 1991-04-10 赵清华 The preparation of edible fungus production increasing agent and using method
CN102090264A (en) * 2009-12-10 2011-06-15 上海市农业科学院 Method for liquid cultivation of hypsizygus marmoreus
CN103168613A (en) * 2011-12-23 2013-06-26 何寒 Fast manufacture method for liquid strain
CN104710206A (en) * 2013-07-12 2015-06-17 鲁东大学 Preparation method for volvariella volvacea liquid strain
CN104261942A (en) * 2014-09-28 2015-01-07 永和县旺达食用菌有限责任公司 Pleurotus nebrodensis liquid strain culture medium and propagation method of strain
CN109006164A (en) * 2018-08-10 2018-12-18 安徽泓森生物科技有限公司 Agricus blazei liquid culture method

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Application publication date: 20210209