CN102296098A - Method for producing high-purity coriolus versicolor polysaccharopeptide by using modern fermentation technology - Google Patents
Method for producing high-purity coriolus versicolor polysaccharopeptide by using modern fermentation technology Download PDFInfo
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Abstract
The invention provides a new modern biological fermentation method for coriolus versicolor polysaccharopeptide. In the method, the amount of coriolus versicolor mycelium is increased by using continuous feeding and controlling fermentation stirring rotation speed during production; meanwhile, a new extraction method is adopted to solve the problems of low yield of coriolus versicolor polysaccharopeptide and the like in the prior art; and thus, the yield and the output of the coriolus versicolor polysaccharopeptide can be effectively improved. In a drying step, a low-temperature drying process is adopted, so that the problem of damage caused to polysaccharopeptide in the coriolus versicolor polysaccharopeptide by the conventional high-temperature dyring technology is solved, and the content of absorbable polysaccharopeptide is increased, meets the requirements of national quality standard and reaches the quality standards of Japanese similar products.
Description
Technical field
The invention belongs to medicinal microbial fermentation field, be specifically related to a kind of production method of using modern biofermentation technique from prepared from coriolus versicolor mycelium, to extract the high purity Polystictus Glycopeptide.
Background technology
Rainbow conk is the higher fungi of Basidiomycetes Aphyllophorales polyporaceae, be traditional Chinese medicine, Compendium of Material Medica put down in writing it and have " strengthen the body resistance to consolidate the constitution, replenishing vital essence and invigorating vital QI, for a long time eat body-building, promote longevity " effect, i.e. the described enhancing body immunologic function of modern medicine, effects such as disease preventing and treating.1970, Japan's scholar first report by extracting a kind of polysaccharide in the mycelium of variegated Coriolous Dersicolor (Fr.) Quel fungus (Coriolus Cariolus Versicolov CM-101), name and be PS-K, its functional component is apparently higher than Coriolous Dersicolor (Fr.) Quel fungus itself, and be easily absorbed by the body, to the experimental therapy of the portable S-180 of mouse, oral or injection is effectively.1976, PS-K at first in Japan's listing, was used for the enhancing body immunologic function, treats diseases such as various malignant tumours, leukemia, chronic hepatitis, liver cirrhosis, obtains better therapeutic effect.
China began variegated rainbow conk is carried out systematic study in 1978; Beginning in 1981 in Chongqing, the medical college on ground such as Beijing, Shanghai and hospital carry out to Polystictus Glycopeptide that (former name: clinical observation on the therapeutic effect krestin) confirms that it has good immunologic enhancement; 1986, we successfully utilized modern biotechnology, adopted submerged fermentation to cultivate and obtained prepared from coriolus versicolor mycelium, and adopt scientific approach to extract its intracellular reactive material, had begun the suitability for industrialized production of China's Polystictus Glycopeptide.
Relevant Chinese patent according to the Polystictus Glycopeptide of announcing at present, it provides the production and the extracting method of multiple Polystictus Glycopeptide, mainly concentrate on the productive rate that improves Polystictus Glycopeptide, adopt to rely on the ratio that improves large molecular weight polysaccharides in the finished product, thereby extract more Polystictus Glycopeptide product.But, relevant pharmacological effect and the clinical study paper delivered according to how tame core medical science of China and pharmaceutical journal show: Polystictus Glycopeptide has the enhancing immune function of human body, the protection liver cell, press down knurl, anti-ageing, anti-infective, antiulcer agent, anti-inflammatory, anti-injury, resisiting influenza virus, reducing blood-fat, analgesia, improve MULTIPLE COMPOSITE effects such as learning and memory function, and after certain Polystictus Glycopeptide sample is hydrolyzed, find, its polysaccharide and protein (peptide) resolve into monose and amino acid, polysaccharide is made up of following 6 kinds of monose: glucose 15.8%, seminose 2.8%, semi-lactosi 2.7%, ribose 1.6%, Fucose 1.2%, wood sugar 1.0%, total reducing sugar amount are 25.1%; Protein is made up of following 15 seed amino acids: the amino acid 2.77% of Radix Asparagi, Threonine 0.74%, Serine 1.01%, L-glutamic acid 4.16%, proline(Pro) 0.48%, glycine 1.7%, L-Ala 1.57%, Gelucystine 0.31%, Xie Ansuan 0.77%, methionine(Met) 0.07%, Isoleucine 0.56%, tyrosine 0.26%, phenylalanine 0.47%, Histidine 0.46%, arginase 12 .79%, total amount is 19.6%; Chemical structure: glucose is many with α-1,4, α-1,2, and β-1,6 glycosidic links and combine based on the small protein (peptide) of L-glutamic acid, the amino acid of Radix Asparagi become a kind of protein-polysaccharide (polysaccharide peptide); And simultaneously, the researchist takes above-mentioned 6 kinds of monose and 15 seed amino acids to the patient clinically, does not but see the effect identical with Polystictus Glycopeptide, and the molecular structure of visible Polystictus Glycopeptide has singularity.Correlative study report according to Hong Kong Chinese University in 2002: real effective component is can be for the peptidoglycan (polysaccharide peptide) of absorption of human body in the Polystictus Glycopeptide, it can pass the human body intestines and internal organs of the body, be absorbed by the body, stimulate the leukocyte cell growth and strengthen other immunologic functions, and the molecule of polysaccharide body is big, be difficult to be absorbed, therefore be difficult to effectively play a role by intestines and internal organs of the body.Therefore, improve the content of the peptidoglycan in the Polystictus Glycopeptide, be only the key that improves this drug product quality and clinical efficacy, the content that improves polysaccharide, particularly large molecular weight polysaccharides does not merely have bigger clinical value.
Therefore, this area is needed the production method of improving Polystictus Glycopeptide badly, improves the purity and the content of peptidoglycan (polysaccharide peptide), makes it the quality requirements that meets the national drug standards and reach Japanese like product.
Summary of the invention
The fermentation process that the purpose of this invention is to provide a kind of new Polystictus Glycopeptide by continuous flow feeding and control fermentation mixing speed, improves the prepared from coriolus versicolor mycelium amount, has improved the effective constituent of intracellular glycopeptide, has improved output simultaneously.
Another object of the present invention provides the extracting method of new Polystictus Glycopeptide, overcoming the low problem of Polystictus Glycopeptide yield in the prior art, thereby improves polysaccharide content.
A further object of the present invention provides the drying process of new Polystictus Glycopeptide, and to overcome the destruction of existing dry technology to polysaccharide body in the Polystictus Glycopeptide, raising can absorb the content of polysaccharide body.
New fermentation process provided by the present invention, its production stage and feature are as follows:
1, bacterial strain incubation step:
From transfer the possession of a strain rainbow conk bacterial strain that obtains, after the screening rejuvenation, obtain a strain strain excellent CVDX-1, this bacterial strain is fit to submerged aerobic fermentation, adaptability to substratum is strong, the soybean cake powder of available cheapness, bean cake powder, groundnut meal are carbon source, and maltose, glucose is carbon source, thereby is the content of the effective ingredient that the improves Polystictus Glycopeptide condition of laying a good foundation.
2, fermentation culture step:
Adopt three grades of aerobic fermentation methods to produce prepared from coriolus versicolor mycelium, the fermentation culture flow process comprises the cultivation of (1) test tube slant; (2) female bottle seed culture; (3) canister is cultivated (one-level); (4) jar cultivation (secondary) in; (5) cultivate (three grades) for big jar.
Concrete enforcement is as follows:
(1) slant culture:
Slant medium: by weight/the volume ratio meter, glucose 2%, yeast extract paste 0.5%, agar 1.5～2%, tap water preparation, PH nature.Sterilized 30 minutes for 119～123 ℃, receive on the substratum, in 25-29 ℃ of incubator, cultivated 6～7 days preserving bacterial strain CVDX-1.
(2) female flask culture:
Substratum is formed: glucose 2.0%, yeast powder 1.0%, twenty propylhomoserin 0.2%, sal epsom 0.5%, zinc sulfate 0.5%, tap water preparation, PH nature.119～123 ℃ of sterilizations 30 minutes, inoculation were placed on culturing room in back and forth on the shaking table (60 times/minute) cultivation 5～6 days, culture temperature: 25～29 ℃.
(3) canister, middle jar of cultivation
Substratum is formed: glucose 1.5%, analysis for soybean powder 1.5%, dextrin 10%, sal epsom 0.5%, potassium primary phosphate 0.5%, lime carbonate 0.2%.Sterilized 30 minutes charging capacity 50～70%, inoculum size 5～10%, 25～29 ℃ of jar temperature, air flow 1: 0.3～1: 0.5 (V/V), incubation time 15～20 hours for 119～123 ℃.
(4) cultivate for big jar
Medium sterilization is 30 minutes under 119～123 ℃ of temperature condition, charging capacity 50～70%, and inoculum size 5～10%, 25～29 ℃ of jar temperature, air flow 1: 0.3～1: 0.5 (V/V), mixing speed 50～200rpm, after the inoculation, mixing speed reduces from high to low gradually.The inoculation back is cultivated between 24～48 hours and is begun feed supplement, and the feed supplement amount is 1～5% of a fermention medium cumulative volume, and feed supplement liquid weight concentration is a glucose 5～10%, analysis for soybean powder 2～4%, peptone 1～2%, incubation time 3～6 days.
New extracting method provided by the present invention, and the new drying process that is provided, its extraction-exsiccant Production Flow Chart and feature comprise: (1) press filtration; (2) hydrolysis; (3) secondary press filtration; (4) concentrate; (5) sedimentation; (6) cryodrying; (7) powder essence; (8) hybrid packed, finished product.
Concrete enforcement is as follows:
1, a press filtration of fermented liquid: fermented liquid is through behind the filter press, and extracellular fluid is arranged trench, and filter cake is transported into hydrolytic decomposition pot and is hydrolyzed.
2, hydrolysis: with tap water, dosage is 3～5 times of filter cake volume, uses steam heating in the hydrolytic decomposition pot, 93～95 ℃ of temperature, and supercharging 0.12～0.15Mpa in jar is incubated 2～3 hours after-filtration.
3, secondary press filtration: hydrolyzed solution is after the pressure filter press filtration, and filtrate is squeezed into filtrate storage bucket and waited to concentrate.
4, concentrate: in concentration tank, adopt the vacuum and steam heating to concentrate, vacuum tightness 0.06～0.07Mpa, concentrated solution concentration reaches 35～65%.
5, sedimentation: in concentrated solution, add to leave standstill after ethanol stirs and took out the alcohol mother liquor in 3～4 hours, xln is carried out drying.
6, crushed after being dried under-40～-30 ℃ temperature is hybrid packed.
Production method of the present invention can be produced the Polystictus Glycopeptide production bacterial classification of high-content effective constituent by adopting the bacterial classification transformation to obtain; Adopt the deep liquid aerobic fermentation, continuous flow feeding and variable-frequency and variable-speed are regulated and are optimized fermenting process during by bulk fermentation, nutritive substances such as the required carbon source of mycelial growth and metabolism, nitrogenous source had both been replenished, again not because of batch feeding destroys mycelial growth and metabolic environment, help effective ingredient synthetic of mycelium intracellular polyse and peptide; Stir by variable-frequency and variable-speed during bulk fermentation simultaneously, rationally regulate the required appearance oxygen of mycelial growth and metabolism, but both saves energy helps the productive rate of mycelium and intracellular polyse and peptide; Extraction process adopts the broken hyphal cell wall of pressurized high-temperature method, at utmost discharges thing in the mycelium cell with the fastest, reduces heating extraction time, keeps the activity of effective constituent, save energy; Adopt low temperature drying technology to obtain finished product, improved the yield of polysaccharide peptide.The present invention helps the synthetic of mycelial growth and Polystictus Glycopeptide, the yield and the quality of Polystictus Glycopeptide have been improved, product total reducing sugar 〉=35%, reducing sugar≤10%, peptide 〉=20%, productive rate reaches 3g/L, and the retention time of main peak is consistent with the main peak retention time of Japanese similar drug PSK, meets the requirement of national drug standards WS-XG-02-2002.
Description of drawings
Fig. 1 is a fermentation culture flow process provided by the invention, and Fig. 2 is extraction-drying process provided by the invention, and Fig. 3 is the HPLC collection of illustrative plates of the Polystictus Glycopeptide of embodiment 1, and Fig. 4 is the HPLC collection of illustrative plates of the Polystictus Glycopeptide of embodiment 2.
Embodiment:
The national drug standards WS-XG-021-2002 of the described Polystictus Glycopeptide of the inventor is referring to " Chinese pharmacopoeia version in 2000, the reference substance/standard substance that are used for the Polystictus Glycopeptide evaluation are provided by Nat'l Pharmaceutical ﹠ Biological Products Control Institute.
Be described further of the present invention below in conjunction with embodiment, should be appreciated that preferred embodiment described herein only is used for specifically setting forth the present invention, and be not used in qualification the present invention.
Embodiment 1
Seed liquor is prepared through two-layer liquid ventilation fermentation culture by the slant culture of bacterial classification CVDX-1.
Slant medium (g/L): glucose 20, yeast extract paste 5, agar 15, tap water preparation, PH nature.119 ℃ of temperature condition were sterilized 30 minutes down, received on the substratum preserving bacterial strain CVDX-, in 25 ℃ of incubators, cultivated 7 days.
Female flask culture base (g/L): glucose 20, yeast powder 10, twenty propylhomoserin 2, sal epsom 5, zinc sulfate 5, tap water preparation, PH nature.119 ℃ of sterilizations 30 minutes, inoculation were placed on culturing room in back and forth on the shaking table (60 times/minute) cultivation 6 days, 25 ℃ of culture temperature.
Canister substratum (g/L): glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.Sterilized 30 minutes for 119 ℃, 10 liters of full-automatic stainless steel fermentor tanks, charging capacity 5L, inoculum size is 5%, 25 ℃ of jar temperature, air flow 1: 0.3 (V/V), incubation time 20 hours.
Cultivate (g/L) for middle jar: glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.Sterilized 30 minutes for 119 ℃, 30 liters of full-automatic stainless steel fermentor tanks, charging capacity 15L, inoculum size is 5%, 25 ℃ of jar temperature, air flow 1: 0.3 (V/V), incubation time 20 hours.
Big jar substratum: glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.500 liters of full-automatic stainless steel fermentor tanks, charging capacity 250L sterilized 30 minutes for 119 ℃, inoculum size 5%, 25 ℃ of jar temperature, air flow 1: 0.3 (V/V), mixing speed 50rpm, after the inoculation, mixing speed reduces from high to low gradually, the inoculation back is cultivated between 48 hours and is begun feed supplement, the feed supplement amount is 1% of a fermention medium cumulative volume, and feed supplement liquid weight concentration (g/L) is a glucose 12.5, analysis for soybean powder 5, peptone 2.5, incubation time 6 days.
Extraction-drying a: press filtration of fermented liquid---fermented liquid is through behind the filter press, and extracellular fluid is arranged trench, and filter cake is transported into hydrolytic decomposition pot and is hydrolyzed; Hydrolysis---with tap water, dosage is 3 times of filter cake volume, uses steam heating in the hydrolytic decomposition pot, 92 ℃ of temperature, and supercharging 0.12Mpa in jar is incubated 3 hours after-filtration; Secondary press filtration---hydrolyzed solution is after the pressure filter press filtration, and filtrate is squeezed into filtrate storage bucket and waited to concentrate; Concentrate---in concentration tank, adopt the vacuum and steam heating to concentrate, vacuum tightness 0.06Mpa, concentrated solution concentration reaches 35%; Sedimentation---in concentrated solution, add to leave standstill after ethanol stirs and took out the alcohol mother liquor in 3 hours, xln is carried out drying under-30 ℃ of temperature.Obtain the Polystictus Glycopeptide of 3g/L, its total reducing sugar is 37.9% after testing, and reducing sugar is 9.1%, peptide 27.8%, HPLC detects, and the retention time of its main peak is consistent with standard substance, and consistent with the main peak retention time of Japanese PS-K sample, meet the requirement of national drug standards WS-XG-02-2002.
Embodiment 2
Seed liquor is prepared through two-layer liquid ventilation fermentation culture by the slant culture of bacterial classification CVDX-1.
Slant medium (g/L): glucose 20, yeast extract paste 5, agar 15, tap water preparation, PH nature.122 ℃ of temperature condition were sterilized 30 minutes down, received on the substratum preserving bacterial strain CVDX-1, in 28 ℃ of incubators, cultivated 5 days.
Female flask culture base (g/L): glucose 20, yeast powder 10, twenty propylhomoserin 2, sal epsom 5, zinc sulfate 5, tap water preparation, PH nature.123 ℃ of sterilizations 30 minutes, inoculation were placed on culturing room in back and forth on the shaking table (60 times/minute) cultivation 5 days, 28 ℃ of culture temperature.
Canister substratum (g/L): glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.Sterilized 30 minutes for 122 ℃, 10 liters of full-automatic stainless steel fermentor tanks, charging capacity 7L, inoculum size is 10%, 28 ℃ of jar temperature, air flow 1: 0.5 (V/V), incubation time 16 hours.
Cultivate (g/L) for middle jar: glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.Sterilized 30 minutes for 122 ℃, 30 liters of full-automatic stainless steel fermentor tanks, charging capacity 21L, inoculum size is 10%, 29 ℃ of jar temperature, air flow 1: 0.5 (V/V), incubation time 16 hours.
Big jar substratum: glucose 15, analysis for soybean powder 15, dextrin 100, sal epsom 5, potassium primary phosphate 5, lime carbonate 2.500 liters of full-automatic stainless steel fermentor tanks, charging capacity 350L sterilized 30 minutes for 122 ℃, inoculum size 10%, 28 ℃ of jar temperature, air flow 1: 0.5 (V/V), mixing speed 100rpm, after the inoculation, mixing speed reduces from high to low gradually, the inoculation back is cultivated between 24 hours and is begun feed supplement, the feed supplement amount is 5% of a fermention medium cumulative volume, and feed supplement liquid weight concentration (g/L) is a glucose 50, analysis for soybean powder 20, peptone 5, incubation time 4 days.
Extraction-drying a: press filtration of fermented liquid---fermented liquid is through behind the filter press, and extracellular fluid is arranged trench, and filter cake is transported into hydrolytic decomposition pot and is hydrolyzed; Hydrolysis---with tap water, dosage is 5 times of filter cake volume, uses steam heating in the hydrolytic decomposition pot, 95 ℃ of temperature, and supercharging 0.15Mpa in jar is incubated 2 hours after-filtration; Secondary press filtration---hydrolyzed solution is after the pressure filter press filtration, and filtrate is squeezed into filtrate storage bucket and waited to concentrate; Concentrate---in concentration tank, adopt the vacuum and steam heating to concentrate, vacuum tightness 0.07Mpa, concentrated solution concentration reaches 60%; Sedimentation---in concentrated solution, add to leave standstill after ethanol stirs and took out the alcohol mother liquor in 3 hours, xln is carried out drying under-40 ℃ of temperature.Obtain the Polystictus Glycopeptide of 3.1g/L, its total reducing sugar is 38.5% after testing, and reducing sugar is 9.4%, peptide 26.2%, HPLC detects, and the retention time of its main peak is consistent with standard substance, and consistent with the main peak retention time of Japanese PS-K sample, meet the requirement of national drug standards WS-XG-02-2002.
The above is the preferred embodiments of the present invention only, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.Within the spirit and principles in the present invention all, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. the preparation method of a Polystictus Glycopeptide is characterized in that, comprises the steps:
After the screening rejuvenation, obtain a kind of strain excellent that is numbered CVDX-1, inoculation CVDX-1 rainbow conk seed culture fluid in fermentor tank, inoculum size 5～10%; 25～29 ℃ of jar temperature, the condition bottom fermentation of air flow 1: 0.3～1: 0.5 (V/V), mixing speed 50～200rpm is cultivated, and cultivates acquisition prepared from coriolus versicolor mycelium fermented liquid 3～6 days.
2. preparation method according to claim 1 is characterized in that, begins feed supplement in initial inoculation culture between 24～48 hours, and the feed supplement amount is 1～5% of a fermention medium cumulative volume.
3. according to the described preparation method of claim 1, it is characterized in that feed supplement liquid weight concentration is a glucose 5～10%, analysis for soybean powder 2～4%, peptone 1～2%.
4. a method of extracting Polystictus Glycopeptide from prepared from coriolus versicolor mycelium is characterized in that, comprises the steps:
The fermented liquid that will contain prepared from coriolus versicolor mycelium is hydrolyzed after the press filtration first time, makes hydrolyzed solution;
Hydrolyzed solution is carried out the press filtration second time, and gained liquid is placed into concentration tank, adopts heating under vacuum to concentrate, and makes the Polystictus Glycopeptide concentrated solution;
After adding ethanol stirs in concentrated solution, left standstill 3～4 hours, take out the alcohol mother liquor, make the Polystictus Glycopeptide xln;
The Polystictus Glycopeptide xln is carried out drying at low temperatures, pulverize, make high purity Polystictus Glycopeptide product.
5. extracting method according to claim 4 is characterized in that, the temperature of described hydrolysis process flow process is at 93～95 ℃, and supercharging 0.12～0.15Mpa in jar is incubated 2～3 hours.
6. extracting method according to claim 4 is characterized in that, described low temperature drying technology flow process ties up under-40～-30 ℃ of conditions carries out.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106589083A (en) * | 2016-12-20 | 2017-04-26 | 江南大学 | Polystictus glycopeptide active ingredient PSK-1c1 for treating alcoholic liver diseases |
| CN108130280A (en) * | 2018-01-26 | 2018-06-08 | 江苏神华药业有限公司 | A kind of Polystictus Glycopeptide active component and its preparation method and application |
| CN109400686A (en) * | 2018-10-23 | 2019-03-01 | 西北农林科技大学 | A kind of extracting method of rainbow conk beta glucan peptide and its application in fat or related disease |
| CN112625915A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for fermenting coriolus versicolor by using airlift fermentation tank fed batch |
| CN112876533A (en) * | 2021-01-18 | 2021-06-01 | 江苏神华药业有限公司 | Preparation method of polysaccharopeptide |
| CN113150063A (en) * | 2021-01-18 | 2021-07-23 | 江苏神华药业有限公司 | Preparation method of novel polysaccharopeptide |
| CN113957109A (en) * | 2021-10-28 | 2022-01-21 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
| CN115232752A (en) * | 2022-05-19 | 2022-10-25 | 江苏神华药业有限公司 | Submerged liquid fermentation method for preparing Coriolus versicolor mycelia and application thereof |
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2010
- 2010-06-28 CN CN2010102102868A patent/CN102296098A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106589083A (en) * | 2016-12-20 | 2017-04-26 | 江南大学 | Polystictus glycopeptide active ingredient PSK-1c1 for treating alcoholic liver diseases |
| CN106589083B (en) * | 2016-12-20 | 2020-04-03 | 江南大学 | Coriolus versicolor glycopeptide active ingredient PSK-1c1 for treating alcoholic liver disease |
| CN108130280A (en) * | 2018-01-26 | 2018-06-08 | 江苏神华药业有限公司 | A kind of Polystictus Glycopeptide active component and its preparation method and application |
| CN109400686A (en) * | 2018-10-23 | 2019-03-01 | 西北农林科技大学 | A kind of extracting method of rainbow conk beta glucan peptide and its application in fat or related disease |
| CN112625915A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for fermenting coriolus versicolor by using airlift fermentation tank fed batch |
| CN112876533A (en) * | 2021-01-18 | 2021-06-01 | 江苏神华药业有限公司 | Preparation method of polysaccharopeptide |
| CN113150063A (en) * | 2021-01-18 | 2021-07-23 | 江苏神华药业有限公司 | Preparation method of novel polysaccharopeptide |
| CN113957109A (en) * | 2021-10-28 | 2022-01-21 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
| CN113957109B (en) * | 2021-10-28 | 2022-09-06 | 江苏骏豪生物科技有限公司 | Industrial green production process of polystictus glycopeptide |
| CN115232752A (en) * | 2022-05-19 | 2022-10-25 | 江苏神华药业有限公司 | Submerged liquid fermentation method for preparing Coriolus versicolor mycelia and application thereof |
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Application publication date: 20111228 |