CN1028435C - Continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix - Google Patents
Continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix Download PDFInfo
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- CN1028435C CN1028435C CN 89104005 CN89104005A CN1028435C CN 1028435 C CN1028435 C CN 1028435C CN 89104005 CN89104005 CN 89104005 CN 89104005 A CN89104005 A CN 89104005A CN 1028435 C CN1028435 C CN 1028435C
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- fermentation
- chinese caterpillar
- caterpillar fungus
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Abstract
Worm grass cephalothin is medicinal fungus and edible fungus which are separated from Chinese caterpillar fungus, and the worm grass cephalothin has the functions of disease treatment and health care. The worm grass cephalothin is generally prepared by an intermittent fermentation technology. The production of the present invention is carried out by a recycled continuous fermentation technology of fermentation by a fermentation tank, material replenishment and ejection outside the tank, and the technology of tank clearing, empty sterilization, material feeding and actual sterilization is canceled. The actual productive time is extended, the output can be increased greatly, the continuous fermentation technology can reach to channelized production, and the opportunity of pollution is reduced; therefore, the quality of products is enhanced.
Description
The present invention is a kind of microbial fermentation engineering without the meta-bolites extraction process, does not need isolating fermentation technique especially for active principles such as glossy ganoderma, armillaria mellea, Cordyceps and thalline.
Chinese caterpillar fungus is to adopt intermittent zymotechnique to produce at present, and process procedure is many, and the middle intermittent time is long, and efficient is low, and energy consumption is big, still can not satisfy the needs of China's medicine industry development.
The objective of the invention is for overcoming the shortcoming of old-fashioned intermittent zymotechnique, reduce process procedure, increase work efficiency, reduce cost, improve product quality and yield, replace original intermittent zymotechnique to satisfy the needs of China's medicine industry development with continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix.
Chinese caterpillar fungus is a strain medicinal fungi of separating from the fresh sample of Cordyceps sinensis, compares with the natural cs composition, and the two has identical composition.Preparatory process procedures such as the clear cylinder in this continuous fermentation process the has been last year intermittent type zymotechnique, material loading, sterilization, cooling, whole process is carried out in closed pipeline equipment, has reduced pollution, has improved quality product and output.
1. the separation of Chinese caterpillar fungus
Adopt the fresh sample in Cordyceps sinensis producing region, go mud, cleaning, through surface sterilization, the epidermis of debug body is avoided enteron aisle, and white sclerotium is cut into small pieces to be arranged in the culture dish that fills substratum, grows white hypha after the week, carries out pure culture at P, D, A substratum.
2. the cultivation of Chinese caterpillar fungus
Through the screening of substratum, cultivate the combination and the culture medium prescription of composition, adopt peptone 2～8g, yeast powder 2～10g, peanut powder 10～20g, sucrose 10～20g, MgSO
40.2～0.5g, KH
2PO
40.3～1g, water 1000ml cultivates 25 ℃～30 ℃ of humidity, through 4 days culture medium culturing, covers with white-colored hairs shape mycelia on whole inclined-plane.
This Chinese caterpillar fungus that is separated to (Cephalosporiom sinensis Yu.et Chen.Sp.nov) is preserved in Chinese typical culture collection center (CCTCC) on June 15th, 1992, and this preserving number is CCTCC NO.M93027.
3. the deep layer of Chinese caterpillar fungus is cultivated
Chinese caterpillar fungus is through the shaking table enlarged culturing of vibrating, and is inoculated into seeding tank and enlarges deep layer and cultivate, and culture medium prescription is a peanut 0.5%～1%, yeast powder 0.3%～0.5%, sucrose 1.5～2.0%, MgSO
40.2～0.5g, KH
2PO
40.3～1g.Through piping, be pressed into through fermentative production in the fermentor tank of culture material sterilization then, through the growth of this bacterium, logarithmic phase before the mycelia aging, goes out jar when meta-bolites reaches the climax.But from growing into peak of growing season, the time is longer, accounts for 2/3 left and right sides time of whole growth phase.And the increase grain weight can significantly shorten during this period of time.Analyze the growth characteristic of this bacterium, and get its thalline, can adopt continuous fermentation process, the zymotechnique schema through test as product characteristics.
The principal feature of this technology is that except that need just to utilize later the interior fermented liquid of fermentor tank as seed for the first time from external world's access seed, certain hour carries out feed supplement at interval.Just go out jar behind the fermentation certain hour, circulation is repeatedly carried out in closed pipeline fully for this reason.
With original intermittent zymotechnique, be to adopt second order fermentation technology, technical process is: shaking table liquid oscilaltion → seeding tank expansion → fermentor tank production → go out jar → drying → packing.Shaking table is cultivated and is wanted 48 hours, seeding tank fermentation 36 hours, fermentor tank goes out jar after fermentation in 48 hours, again through clear jar, material loading, sterilization, operations such as cooling need 12 hours, want 148 hours altogether, and continuous fermentation process are continuous feed supplements, constantly go out jar, cycle is 8 hours, has reduced process procedure, has shortened the time.In addition, removed clear jar with continuous fermentation process, material loading, sterilization, preparatory process procedures such as cooling, whole process is carried out in closed pipeline equipment, has reduced opportunities for contamination, has reduced cost, has improved the quality and the output of product.In addition, the Chinese caterpillar fungus body is the new active drug of treatment refractory arrhythmia, and to the treatment bradyarrhythmia, this medicine has one's own knack.Through expert statement, this medical instrument has health care, the effect of nourishing.Because of natural cs costs an arm and a leg, isolate Cordyceps and carry out fermentative production, can satisfy the medicine for treatment of society, the needs of health care medication constantly improve the mode of production simultaneously, can constantly improve product quality and yield.As seen, the series product of this medicine of mass production to development China medicine industry, are guaranteed the healthy of broad masses of the people, and are significant.
Embodiment:
Adopt the fresh sample in Cordyceps sinensis producing region, remove mud, clean, through surface sterilization, the epidermis of debug body is avoided enteron aisle, and white sclerotium is cut into small pieces to be arranged in the culture dish that fills substratum, grows white hypha after the week, carries out pure culture at P, D, A substratum.Chinese caterpillar fungus is potato, and glucose on the agar, was cultivated 5 days in 28 ℃, the bacterium colony circle, and neat in edge, smooth, tightr, white, fine hair is the short flannel shape, does not have the pigment that is spread in substratum.Conidiophore is transparent, directly or curved slightly, single giving birth to or 2～3 consor, but be not colyliform, end or cardinal extremity do not have obviously and expand, 15-40 * 1.5-2 μ m, the conidium head is false head, diameter 20 * 35 μ m, form by about 20 conidium adhesive aggregations, conidium is colourless, monospore, oval 2.5～6(8) * 1.5～1.8(2) μ m.
Chinese caterpillar fungus fermentative medium formula: peanut powder 5g, yeast 10g, sucrose 15g, MgSO
40.5g, KH
2PO
41g, pH6.5-6.0, water 1000ml, behind the fermentation certain hour, begin to produce falx and spore at above-mentioned substratum, owing to constantly stir in the fermenting process, so the conidial head that produces on the sporophore is only deposited a spore, i.e. conidium on sporophore.Along with fermentation time increases, mycelial density increases, and the pH value descends, and produces meta-bolites in a large number, through tests such as reducing sugar and ammonia attitude fluorine, goes out jar after fermentation is finished.
The culture medium prescription that the Chinese caterpillar fungus deep layer is cultivated: peanut powder 5g, yeast 10g, sucrose 15g, MgSO
40.5g, KH
2PO
41g, the seed of pH6.5-6.0, water 1000ml fermenting-ripening in the seeding tank moves in the fermentor tank, after fermentation is finished, take out a certain amount of fermented liquid, mend nutrient solution, continue fermentation, for this reason repeatedly with amount, constantly feed supplement constantly goes out jar, and whole fermentation technology process is a successive.
Claims (2)
1, continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix, it is characterized in that Chinese caterpillar fungus (Cephologporiumginensis Yu.et Chen.Sp.Nov) (CCTCC NO:M93027) fermentation culture after 48 hours, emit a certain amount of fermented liquid, mend with the amount culture material, continue fermentation, constantly go out a jar feed supplement so repeatedly, the cycle is 8 hours.
2, zymotechnique according to claim 1, the prescription that it is characterized in that fermention medium are peanut powder 5 grams, yeast 10 grams, sucrose 15 grams, MgSO
40.5 gram, KH
2PO
41 gram, pH6.5-6.0, water 1000ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89104005 CN1028435C (en) | 1989-06-13 | 1989-06-13 | Continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89104005 CN1028435C (en) | 1989-06-13 | 1989-06-13 | Continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1040391A CN1040391A (en) | 1990-03-14 |
| CN1028435C true CN1028435C (en) | 1995-05-17 |
Family
ID=4855383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 89104005 Expired - Fee Related CN1028435C (en) | 1989-06-13 | 1989-06-13 | Continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1028435C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065569C (en) * | 1996-05-23 | 2001-05-09 | 崔照有 | Method for fermentation of edible mushroom activated plant magma enzyme and its products |
| CN1079204C (en) * | 1997-10-07 | 2002-02-20 | 赵铨 | Chinese caterpillar fungus feeds additive and its prodn. method |
| CN100374542C (en) * | 2003-08-19 | 2008-03-12 | 俞永信 | Cordyceps vegetative stage continuous fermentation technology |
| CN109355204A (en) * | 2018-11-28 | 2019-02-19 | 马兰 | A kind of method of fermenting and producing cordyceps sinensis mycelium powder |
-
1989
- 1989-06-13 CN CN 89104005 patent/CN1028435C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1040391A (en) | 1990-03-14 |
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| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yu Yongxin Inventor after: Fang Liangzhen Inventor after: Zhu Haiying Inventor before: Yu Yongxin Inventor before: Fang Liangzhen |
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| COR | Change of bibliographic data |
Free format text: CORRECT: PATENTEE; FROM: YU YONGXIN; FANG LIANGZHEN TO: YU YONGXIN; FANG LIANGZHEN; ZHU HAIYING; INVENTOR; FROM: YU YONGXIN; FANG LIANGZHEN TO: YU YONGXIN; FANG LIANGZHEN; ZHU HAIYING |
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| CP01 | Change in the name or title of a patent holder |
Patentee after: Yu Yongxin Patentee after: Fang Liangzhen Patentee after: Zhu Haiying Patentee before: Yu Yongxin Patentee before: Fang Liangzhen |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |