JP2001017158A - Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same composition - Google Patents
Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same compositionInfo
- Publication number
- JP2001017158A JP2001017158A JP11181931A JP18193199A JP2001017158A JP 2001017158 A JP2001017158 A JP 2001017158A JP 11181931 A JP11181931 A JP 11181931A JP 18193199 A JP18193199 A JP 18193199A JP 2001017158 A JP2001017158 A JP 2001017158A
- Authority
- JP
- Japan
- Prior art keywords
- mycelium
- culture
- mulberry
- medium
- brown rice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、菌類の培養技術に
関する。[0001] The present invention relates to a technique for culturing fungi.
【0002】[0002]
【従来の技術】桑黄(メシマコブ;Phellinus linteu
s)は桑木の枯木に稀に自生するメシマコブで、キノコ
類の中で最強の抗腫瘍性があるものと報告されている。BACKGROUND OF THE INVENTION Phellinus linteu
s) is a rare pear tree that grows naturally on dead mulberry trees, and is reported to have the strongest antitumor properties among mushrooms.
【0003】現在、癌の治療には、化学療法、放射線療
法、あるいは手術による外科的療法などがあるが、その
治療範囲は、治療限界や副作用のため制限的である。そ
こで、直接的な細胞毒性を有する既存の化学療法による
治療に代えるか、あるいは並行させて、宿主の癌細胞に
対する免疫活性を復活させるようにし、人体に無害でよ
り効率のよい治療が試されてきている。[0003] Currently, cancer treatment includes chemotherapy, radiation therapy, or surgical treatment by surgery, but the treatment range is limited due to therapeutic limitations and side effects. Therefore, alternative or parallel treatment with existing chemotherapy that has direct cytotoxicity has been tried to restore the host's immune activity against cancer cells, and a more efficient treatment that is harmless to the human body has been tried. ing.
【0004】既に、BCGなどの細菌製剤とサッカロミ
セスセレビシア(Saccaromyces cerevisiae)の細胞壁
多糖であるザイモサン(Zymosan)が、宿主の免疫性を
刺激して抗癌性作用を示すものと知られている。また、
漢方で用いられてきた菌類生薬、地衣類生薬、各種植物
性生薬から分離したリポポリサッカライド(lipopolysa
ccaride)、プロテオグリカン(proteoglycan)、ヘミ
セルロース(hemicellulose)が宿主媒介性抗腫瘍作用
を有すると報告されている。最近では、椎茸からのレン
チナン(Lentinan)、雲芝からのPSK及びコポラング
(copolang)、プロテオグリカンなど担子菌類から分離
した多糖又は蛋白多糖の含瘍活性が明らかにされ、抗腫
瘍免疫療法剤として用いられるか開発されている。It is already known that bacterial preparations such as BCG and Zymosan, a cell wall polysaccharide of Saccaromyces cerevisiae, exhibit anticancer effects by stimulating host immunity. Also,
A lipopolysaccharide (lipopolysa) isolated from fungal, lichen and various botanical herbs used in Chinese medicine
ccaride), proteoglycan, and hemicellulose have been reported to have host-mediated antitumor effects. Recently, the lactolytic activity of polysaccharides or protein polysaccharides isolated from basidiomycetes, such as Lentinan from Shiitake mushrooms, PSK from Koshiba, copolang, and proteoglycan, has been revealed and used as an antitumor immunotherapy. Or has been developed.
【0005】イケカワ(Ikekawa)などは、このような
担子菌類の抗腫瘍性多糖類についての比較研究の結果、
桑黄が最も強力な宿主媒介性抗腫瘍効果を有すると報告
したことがある。しかし、桑黄は桑木の枯木に寄生する
キノコで、自然界で自生するものが極めて少なく、桑木
の減少とともに桑黄子実体の採取が困難になっており、
資源性がなく、人工培養も極めて困難であって、今日ま
で殆ど実用化されていない。[0005] Ikekawa et al. Have reported a comparative study on such anti-tumor polysaccharides of basidiomycetes,
We have reported that Mulberry has the strongest host-mediated antitumor effect. However, mulberry yellow is a mushroom that is parasitic on dead mulberry trees, and there are very few that grow naturally in the natural world, and as mulberry trees decrease, it becomes difficult to collect mulberry yellow fruit bodies,
It lacks resources and is extremely difficult to artificially culture, and has not been practically used until today.
【0006】[0006]
【発明が解決しようとする課題】桑黄菌糸体の培養と抽
出方法としては、桑黄の子実体を採取し、菌糸体を寒天
培地に分離培養した後、鋸屑1,000g、大豆粉25
0g、ペプトン3.0g、KNO35.0g、CaCO
37.5g、蒸留水1.8〜2.0リットルから構成さ
れる固形培地に菌糸体を接種し、インキュベーターによ
り25℃で3ヵ月間培養してから、乾燥させた菌糸塊1
00gを1,000mlの熱水で1時間環流抽出し、抽出
液を100℃以下の低温で100mlまで減圧濃縮した
後、濃縮液を凍結乾燥して、粉末を得る方法がある。As a method of culturing and extracting the mycelium of Mulberry, the fruit body of Mulberry was collected, and the mycelium was separated and cultured on an agar medium.
0 g, peptone 3.0 g, KNO 35.0 g, CaCO
The mycelium was inoculated on a solid medium composed of 37.5 g and 1.8 to 2.0 liters of distilled water, cultured at 25 ° C. for 3 months in an incubator, and then dried in a hyphal mass 1
There is a method in which 00 g is reflux-extracted with 1,000 ml of hot water for 1 hour, the extract is concentrated under reduced pressure at a low temperature of 100 ° C. or less to 100 ml, and the concentrated liquid is freeze-dried to obtain a powder.
【0007】しかし、この従来の桑黄菌糸体の培養方法
は、菌糸体を医薬用に使用するための方法であるため、
菌糸体の培養及び抽出方法がややこしく、抽出収率が高
いとは言えない。また、培養時に培地組成成分として食
用不可のものを使用するため、別の培地を製造すべきで
あるうえ、培地成分を利用し得ないので菌糸体の培養及
び抽出に高い費用がかかる問題点がある。However, this conventional method of cultivating Mulberry mycelium is a method for using the mycelium for pharmaceuticals.
The method of culturing and extracting mycelium is complicated, and the extraction yield cannot be said to be high. In addition, since a non-edible medium component is used during the culture, another medium must be manufactured, and the medium component cannot be used, so that the cost of culturing and extracting mycelium is high. is there.
【0008】したがって本発明の目的は、桑黄菌糸体の
培養が容易であり、別の菌糸体抽出工程を不要とした桑
黄菌糸体培養用培地組成物を提供することにある。そし
て、その桑黄菌糸体培養用培地組成物を用いた桑黄菌糸
体の製造方法を提供するものである。Accordingly, it is an object of the present invention to provide a medium composition for mulberry mycelium culture in which cultivation of mulberry mycelium is easy and a separate mycelium extraction step is not required. Further, the present invention provides a method for producing a Mulberry mycelium using the medium composition for culture of Mulberry mycelium.
【0009】[0009]
【課題を解決するための手段】本発明によれば、玄米、
炒た玄米、麦、麦粉又は炒た丸麦の中の少なくとも1種
40〜60wt%(重量%)と水40〜60wt%とから構成
され、培養用容器に入れて、たとえば所定の蒸気圧(1
5lb/cm2,120〜121℃)で所定の時間(50〜
60分間)加熱して高圧滅菌し、冷却することで培地組
成物を得ることを特徴とする。そして、これによる培地
に、自然産桑黄の子実体から組織を分離し桑木抽出培地
で継代培養した後に同様の培地で種培養した種培養液を
接種し、たとえば25℃±1℃の所定温度で25〜30
日間などの所定期間培養する培養方法を提供する。According to the present invention, brown rice,
It is composed of at least one of roasted brown rice, wheat, barley flour or roasted barley and 40 to 60% by weight of water and 40 to 60% by weight of water.
5 lb / cm 2 , 120-121 ° C) for a predetermined time (50-
It is characterized in that a medium composition is obtained by heating, autoclaving, and cooling. Then, the medium is inoculated with a seed culture obtained by separating tissues from naturally occurring mulberry fruit bodies, subcultured on a mulberry tree extraction medium, and then seed-cultured on the same medium, for example, at 25 ° C. ± 1 ° C. 25-30 at temperature
Provided is a culturing method for culturing for a predetermined period such as days.
【0010】これにより、桑黄菌糸体を容易に短時間、
高収率で培養し得るうえ、単純な乾燥方法で粉末化して
培地成分とともに2次使用することができるので、経済
的であり、種々の栄養分を多く含有した桑黄菌糸体を得
ることができる。[0010] Thereby, the mycelium of Mulberry can easily be prepared for a short time,
In addition to being able to be cultured at a high yield, it can be powdered by a simple drying method and can be used secondarily together with the medium components, so that it is economical and can obtain Mulberry mycelium containing various nutrients in a large amount. .
【0011】[0011]
【発明の実施の形態】本例の桑黄菌糸体培養用培地組成
物は、玄米、炒た玄米、麦、麦粉又は炒た丸麦の中の少
なくとも1種40〜60wt%と水(地下水)40〜60w
t%とから構成し、種菌培養用容器(瓶)に入れて高圧滅
菌法で滅菌し冷却して得る。そして、この桑黄菌糸体培
養用培地組成物を用いた本例の桑黄菌糸体培養方法は、
自然産桑黄の子実体から組織を分離し桑木抽出培地で継
代培養した後に同様の桑黄菌糸体培養用培地で種培養し
た種培養液を、本例の桑黄菌糸体培養用培地に接種し、
インキュベーター内で25℃±1℃の温度で25〜30
日間培養するものである。BEST MODE FOR CARRYING OUT THE INVENTION The medium composition for mulberry mycelium culture of this example comprises 40 to 60% by weight of at least one of brown rice, roasted brown rice, barley, flour or roasted barley, and water (groundwater) 40. ~ 60w
%, and put in a container (bottle) for inoculum culture and sterilized by high-pressure sterilization and cooled. And the method of cultivating Mulberry mycelium of this example using the medium composition for Mulberry mycelium culture,
After separating the tissue from the fruiting body of naturally occurring mulberry and subculturing it in mulberry tree extraction medium, the seed culture solution cultivated in the same mulberry mycelium culture medium was added to the mulberry mycelium culture medium of this example. Inoculate,
25-30 ℃ at the temperature of 25 ℃ ± 1 ℃ in the incubator
They are cultured for days.
【0012】玄米は、稲の搗精過程(脱穀)で、籾殻の
みを剥き、白糠はそのままに残した米を意味するもの
で、搗精度が20%未満のものを使用することが好まし
い。これは、一般に市販されている玄米を用いることで
可能である。搗精度が20%以上のものを使用する場合
は、桑黄菌糸体の培養が十分にできない可能性がある。Brown rice is a rice milling process (threshing) in which only rice husks are peeled off, and white bran means rice left as it is, and it is preferable to use one with a grinding accuracy of less than 20%. This is possible by using brown rice which is generally commercially available. When a milling accuracy of 20% or more is used, there is a possibility that the mulberry mycelium cannot be sufficiently cultured.
【0013】炒た玄米は、玄米を100℃の温度で5〜
10分間十分に掻きまわしながら加熱するか、加熱室で
温度を徐々に上昇させ83〜85℃を3〜4時間維持す
る手法を使用する。炒た玄米は相対的に玄米より水分含
量が低いので、培地造成時に水分量を多くするのがよ
い。[0013] The roasted brown rice is prepared by heating brown rice at a temperature of 100.degree.
Heat while stirring thoroughly for 10 minutes, or use a technique of gradually increasing the temperature in a heating chamber and maintaining 83-85 ° C. for 3-4 hours. Since roasted brown rice has a relatively lower moisture content than brown rice, it is preferable to increase the moisture content when forming the medium.
【0014】麦は、搗精度が30%のものを使用すると
好ましい。搗精度が30%を超える場合は、玄米の搗精
度が20%以上である場合と同様に桑黄菌糸体の培養程
度が足りなくなる可能性がある。It is preferable to use wheat having a grinding accuracy of 30%. When the milling accuracy exceeds 30%, there is a possibility that the degree of cultivation of Mulberry yellow mycelium may be insufficient as in the case where the milling accuracy of brown rice is 20% or more.
【0015】炒た麦も、炒た玄米と同手法で処理したも
のとすることができる。[0015] The roasted wheat can also be treated in the same manner as roasted brown rice.
【0016】丸麦は、通常の麦より搗精度の低いもの
で、籾殻のみを剥いたものを使用する。Barley has a lower milling accuracy than ordinary wheat, and is obtained by stripping only chaff.
【0017】以上の玄米、炒た玄米、麦、炒た麦、丸麦
が桑黄菌糸体培養用培地成分として好適な理由は、白糠
又は麦芯内の栄養成分の一部成分が桑黄菌糸体の成長に
必須成分として作用するものであるからと判断される。
これは、白米を培地成分として使用する場合は桑黄菌糸
体の培養ができないか、足りないという結果から推測し
得るものである。The reason why the above brown rice, roasted brown rice, barley, roasted barley, and barley are suitable as a medium component for mulberry mycelium culture is that some of the nutrients in white bran or wheat core are mulberry mycelium. It is determined that the compound acts as an essential component for the growth of.
This can be guessed from the results that when white rice is used as a medium component, cultivation of mulberry mycelium is not possible or is insufficient.
【0018】玄米、炒た玄米、麦、炒た麦、丸麦の使用
比率が40wt%未満であるか、地下水の使用比率が60w
t%を超える場合は、桑黄菌糸体の培養期間が長くなった
り、桑黄菌糸体の培養が足りなくなる。一方、玄米、炒
た玄米、麦、炒た麦、丸麦の使用比率が60wt%を超え
るか、地下水の使用比率が40wt%未満である場合は、
培地の状態が乾燥し、桑黄菌糸体の培養がうまくできな
いか、菌糸密度が低下してしまい、収得される菌糸体量
が減少する。The usage ratio of brown rice, roasted brown rice, wheat, roasted barley, barley is less than 40% by weight, or the usage ratio of groundwater is 60w
When the amount exceeds t%, the culture period of the Mulberry mycelium is prolonged, or the culture of the Mulberry mycelium becomes insufficient. On the other hand, if the usage ratio of brown rice, roasted brown rice, wheat, roasted barley, barley exceeds 60 wt%, or the usage ratio of groundwater is less than 40 wt%,
The state of the culture medium is dried, and the cultivation of Mulberry mycelium is not successful, or the density of mycelium is reduced, and the amount of mycelium obtained is reduced.
【0019】玄米、炒た玄米、麦、炒た麦、丸麦と地下
水を種菌培養用瓶に入れて高圧滅菌することにより、滅
菌効果に加え、玄米、炒た玄米、麦、炒た麦、丸麦の蒸
熟が行われて、桑黄菌糸体が容易に成長し得る。滅菌
は、高圧滅菌装置(autoclave)を用いて15lb/cm2の
蒸気圧(約120℃)で15〜20分間加熱することで
滅菌を行う高圧滅菌法において、加熱時間を50〜60
分間に延長することにより、玄米、炒た玄米、麦、炒た
麦、丸麦の内部で成長し得る各種微生物を滅菌する手法
とする。Brown rice, roasted brown rice, barley, roasted barley, barley and groundwater are put into a seed culture bottle and sterilized by high pressure to obtain a sterilizing effect. In addition to the sterilization effect, brown rice, roasted brown rice, barley, roasted barley, barley Is carried out, so that the mycelium of Mulberry can easily grow. Sterilization is performed by using a high-pressure sterilizer (autoclave) at a steam pressure of 15 lb / cm 2 (about 120 ° C.) for 15 to 20 minutes to perform sterilization by heating for 50 to 60 minutes.
It is a technique to sterilize various microorganisms that can grow inside brown rice, roasted brown rice, barley, roasted barley, and barley by extending the length to minutes.
【0020】使用する菌株は、自然産桑黄の子実体から
組織を分離した後、桑木抽出培地で継代培養し、所定の
容器として250mlの三角フラスコに玄米50gと水5
0ccを注入して高圧殺菌(121℃/1.2kg/50
分)を実施し冷却した培地に、前記継代培養液を接種し
て培養したものを培養接種源として使用する。The strain to be used is obtained by isolating tissues from naturally occurring mulberry fruit bodies, subculturing them in a mulberry tree extraction medium, and placing 50 g of brown rice and water 5 in a 250 ml Erlenmeyer flask as a predetermined container.
0cc and autoclaved (121 ° C / 1.2kg / 50
The culture obtained by inoculating the above-mentioned subculture solution into the cooled medium after cooling is used as a culture inoculation source.
【0021】本例に用いられる自然産桑黄は、韓国、江
原道の雪岳山で自生するものを採取したものである。こ
れを使用して通常の方法により子実体から組織を分離
し、桑木の枯木を材料とする固形培地で停止培養方法に
より継代培養する。そして、250mlの三角フラスコに
玄米50gと水50ccを注入し、高圧殺菌(121℃/
1.2kg/50分)を実施して冷却した培地に、前記継
代培養された菌糸体を無菌箱又は無菌室という無菌状態
で接種して種培養し、これを接種源として使用した。The naturally produced mulberry yellow used in the present example is obtained from those native to Mt. Seorak in Gangwon-do, Korea. Using this, tissue is separated from the fruiting body by a usual method, and subcultured by a stop culture method in a solid medium using dead mulberry trees as a material. Then, 50 g of brown rice and 50 cc of water are poured into a 250 ml Erlenmeyer flask, and sterilized by high pressure (121 ° C. /
(1.2 kg / 50 min) and cooled, and the subcultured mycelium was inoculated in a sterile state such as a sterile box or sterile room into a seed medium for seed culture, and used as an inoculum source.
【0022】種培養液の接種量は、培地の中の玄米、炒
た玄米、麦、炒た麦、丸麦の重量に対し1〜10%とす
ることが好ましい。そして、桑黄菌糸体の培養条件は2
5±1℃の温度とし、肉眼で観察して桑黄菌糸体が種菌
培養瓶内いっぱいに満ちるまで培養する。The inoculum amount of the seed culture solution is preferably 1 to 10% based on the weight of brown rice, roasted brown rice, barley, roasted barley, and barley in the medium. The culture conditions for Mulberry mycelium were 2
The temperature is set to 5 ± 1 ° C., and the cells are cultured with naked eyes until the mulberry mycelium is completely filled in the inoculum culture bottle.
【0023】次に、さらなる具体例をあげて説明する
が、これは本発明の範囲を限定するものではない。Next, the present invention will be described with reference to further specific examples, which do not limit the scope of the present invention.
【0024】第1例:First example:
【0025】玄米(農協製)300gと地下水250cc
を1,000mlの種菌培養用瓶に入れて蓋を閉じ、高圧
滅菌装置を用いた高圧滅菌法(15lb/cm2の蒸気圧1
20〜121℃で60分間)により滅菌した後に冷却す
ることで培地を得る。一方、韓国江原道の雪岳山で自生
する自然産桑黄を採取して通常の方法で子実体から組織
を分離した後、桑木の枯木を材料とする固形培地で停止
培養方法で継代培養し、この継代培養された菌糸体を、
250mlの三角フラスコに玄米50gと水50ccを注入
して高圧殺菌(121℃/1.2kg/50分)を実施し
冷却した培地に無菌室で接種する。これにより種培養し
た種培養液15gを、前記の培地に接種し、25±1℃
の温度で培養した。300 g of brown rice (produced by the agricultural cooperative) and 250 cc of groundwater
Was placed in a 1,000 ml inoculum culture bottle, the lid was closed, and a high-pressure sterilization method using a high-pressure sterilizer (vapor pressure of 15 lb / cm 2) was performed.
(20-121 ° C. for 60 minutes), followed by cooling to obtain a culture medium. On the other hand, natural mulberry that grows naturally at Mt.Seorak in Gangwon-do, Korea, is collected, tissues are separated from fruiting bodies by the usual method, and then subcultured by solid culture using dead wood of mulberry as a stop culture method. , This subcultured mycelium,
50 g of brown rice and 50 cc of water are poured into a 250 ml Erlenmeyer flask, subjected to high-pressure sterilization (121 ° C./1.2 kg / 50 minutes), and the cooled medium is inoculated in a sterile room. 15 g of the seed culture obtained by the seed culture is inoculated into the above-mentioned medium, and the temperature is 25 ± 1 °
At the following temperature.
【0026】その後の菌糸体の培養率を測定し、菌糸密
度を肉眼で評価して表1に記載してある。菌糸培養率
は、30日経過後に100%であり、菌糸密度は高かっ
た。The cultivation rate of the mycelium was measured thereafter, and the density of the mycelium was visually evaluated. The hyphal culture rate was 100% after 30 days, and the hyphal density was high.
【0027】第2〜18例及び第1〜5比較例:Examples 2 to 18 and Comparative Examples 1 to 5:
【0028】培地成分の種類と量を表1のように変更し
たことを除き第1例と同じ方法とした。その桑黄菌糸を
培養しつつ菌糸体の培養率と菌糸密度を測定及び評価し
て表1に記載してある。The same method as in Example 1 was used except that the types and amounts of the medium components were changed as shown in Table 1. The culture rate and hyphal density of the mycelium were measured and evaluated while culturing the mulberry mycelium, and the results are shown in Table 1.
【表1】 [Table 1]
【0029】表1から分かるように、玄米、炒た玄米、
麦、炒た麦、丸麦の中の少なくとも1種と地下水とを高
圧滅菌した培地は、培地成分による菌糸生長及び菌糸密
度に大差ない。さらに、炒た丸麦では空隙率が高くて菌
糸密度が高い。一方、炒た丸トウモロコシは、殺菌時に
種菌瓶の内部の水が沸き立って蓋の内面に接するため、
培養種が汚染されることが分かる。As can be seen from Table 1, brown rice, roasted brown rice,
A medium in which at least one of wheat, roasted barley, and barley and groundwater are autoclaved has no significant difference in hyphal growth and hyphal density due to the medium components. Furthermore, roasted barley has a high porosity and a high mycelium density. On the other hand, roasted round corn comes into contact with the inner surface of the lid because the water inside the inoculum bottle boils up during sterilization,
It can be seen that the cultured species is contaminated.
【0030】また、白米を使用して第1例と同じ方法で
桑黄菌糸体を培養した結果、培地の栄養成分の枯渇によ
り、桑黄菌糸体の培養は思うように進まなかった。さら
に、滅菌を行わなかった場合には雑菌の成長により汚染
され、蒸留水を使用する場合は、無機物が不足して菌糸
体の培養がうまくできなかった。Further, as a result of cultivating Mulberry mycelium using the white rice in the same manner as in the first example, the culture of Mulberry mycelium did not proceed as expected due to the depletion of nutrient components in the medium. Furthermore, when sterilization was not performed, contamination was caused by the growth of various bacteria, and when distilled water was used, the mycelium could not be cultured successfully due to a lack of inorganic substances.
【0031】第17〜19例:Seventeenth to nineteenth examples:
【0032】種培養液の接種量を表2のように変更した
ことを除き、第1例と同じ方法で桑黄菌糸体を培養し
た。その菌糸密度をスペクトロフォトメーターで測定し
た結果、第1例の場合は25日目に培養が完了され、第
17〜19例の場合は表2のような結果を得た。The mycelium of Mulberry was cultured in the same manner as in Example 1 except that the inoculum amount of the seed culture was changed as shown in Table 2. As a result of measuring the hyphal density with a spectrophotometer, the culture was completed on the 25th day in the case of the first example, and the results shown in Table 2 were obtained in the case of the 17th to 19th examples.
【表2】 [Table 2]
【0033】腫瘍培養液の接種量は、培地固形分重量に
対し1〜10wt%が好ましく、1,000cc種菌培養用
瓶に最も効果的な培地は、玄米300gと地下水250
ccとから構成される培地であった。The inoculation amount of the tumor culture solution is preferably 1 to 10% by weight based on the weight of the solid content of the medium. The most effective medium for a 1,000 cc culture bottle is 300 g of brown rice and 250 g of groundwater.
cc.
【0034】[0034]
【発明の効果】本発明の桑黄菌糸体培養用培地組成物及
びこれを用いる桑黄菌糸体の培養方法は、容易に得られ
る穀物類を培地成分として使用するので、桑黄菌糸体を
高密度で容易に得ることができるだけでなく、培養完了
後、培地成分とともに桑黄菌糸体を用いることが可能と
なり、桑黄菌糸体の抽出剤が不要であって、経済的であ
り、菌糸体の培養工程が簡単である。Industrial Applicability The medium composition for mulberry mycelium culture of the present invention and the method for culturing the mulberry mycelium using the same use cereals that can be easily obtained as a medium component, and thus the mulberry mycelium can be produced at a high level. Not only can it be easily obtained in density, but also after completion of cultivation, it becomes possible to use Mulberry mycelium together with the medium components, and there is no need for an extractant for Mulberry mycelium, which is economical and reduces the mycelium. The culturing process is simple.
フロントページの続き Fターム(参考) 2B011 AA07 BA08 BA13 GA04 GA12 KA04 4B065 AA71X BB26 BC36 BD08 CA44 Continued on the front page F term (reference) 2B011 AA07 BA08 BA13 GA04 GA12 KA04 4B065 AA71X BB26 BC36 BD08 CA44
Claims (2)
の中の少なくとも1種40〜60wt%と水40〜60wt%
とから構成され、培養用容器に入れて高圧滅菌し冷却し
て得る桑黄菌糸体培養用培地組成物。1. 40 to 60% by weight of at least one of brown rice, roasted brown rice, barley, flour or roasted barley and 40 to 60% by weight of water.
And a medium composition for cultivation of Pseudomonas aeruginosa mycelium obtained by putting into a culture vessel, sterilizing under high pressure, and cooling.
枯木を材料とする固形培地により停止培養方法で継代培
養した菌糸体を、250mlの容器に玄米50g及び水5
0ccを入れて高圧殺菌(121℃/1.2kg/50分)
し冷却して得た培地に無菌状態で接種して種培養し、こ
れによる種培養液を、請求項1の桑黄菌糸体培養用培地
組成物による培地に接種して培養することを特徴とする
桑黄菌糸体の培養方法。2. A mycelium obtained by separating tissue from the fruit body of Mulberry yellow and subcultured by a stop culture method using a solid medium containing dead wood of Mulberry tree, and adding 50 g of brown rice and 50 g of water to a 250 ml container.
Add 0cc and pasteurize (121 ℃ / 1.2kg / 50min)
The culture medium obtained by cooling and cooling is aseptically inoculated and seed-cultured, and the seed culture thus obtained is inoculated and cultured on the medium comprising the medium composition for mulberry mycelium culture of claim 1. A method for culturing the mycelium of Mulberry.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11181931A JP2001017158A (en) | 1999-06-28 | 1999-06-28 | Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same composition |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11181931A JP2001017158A (en) | 1999-06-28 | 1999-06-28 | Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same composition |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2001017158A true JP2001017158A (en) | 2001-01-23 |
Family
ID=16109409
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP11181931A Withdrawn JP2001017158A (en) | 1999-06-28 | 1999-06-28 | Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same composition |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2001017158A (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20010088514A (en) * | 2001-07-31 | 2001-09-28 | 장현유 | A cultivation medium composition of mushroom mycelium containing a roasted grain particle |
KR20040024757A (en) * | 2002-09-16 | 2004-03-22 | 이영무 | How to rapidly nourish phellinus linteus mycelium by the grains |
KR100889930B1 (en) | 2008-08-27 | 2009-03-24 | 이태봉 | Method of mass-culturing inonotus obliquus and phellinus linteus using cereals or medical plants, food comprising the inonotus obliquus and the phellinus linteus cultured thereby, and method of manufacturing the food |
KR100900407B1 (en) | 2003-12-12 | 2009-06-02 | 광동제약 주식회사 | A manufacturing process for culture of Phellinus Linteus |
CN102599006A (en) * | 2012-02-25 | 2012-07-25 | 何寒 | Rapid preparation method for edible fungi grain strain |
JP2016077283A (en) * | 2014-10-13 | 2016-05-16 | 農業会社法人自然生命代替医学(株) | Fermented zen food composition comprising mixture of agricultural product and cereal mycelium, for preventing cancer and diabetes and for enhancing immunity, and production method thereof |
CN105993594A (en) * | 2016-05-24 | 2016-10-12 | 宜州市廖哥山食用菌农民专业合作社 | Method for cultivating mulberry branch auricularia polytricha with silkworm-raising room |
CN113812304A (en) * | 2021-11-05 | 2021-12-21 | 桂东森宜食用菌发展有限公司 | Edible mushroom inoculation method |
-
1999
- 1999-06-28 JP JP11181931A patent/JP2001017158A/en not_active Withdrawn
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20010088514A (en) * | 2001-07-31 | 2001-09-28 | 장현유 | A cultivation medium composition of mushroom mycelium containing a roasted grain particle |
KR20040024757A (en) * | 2002-09-16 | 2004-03-22 | 이영무 | How to rapidly nourish phellinus linteus mycelium by the grains |
KR100900407B1 (en) | 2003-12-12 | 2009-06-02 | 광동제약 주식회사 | A manufacturing process for culture of Phellinus Linteus |
KR100889930B1 (en) | 2008-08-27 | 2009-03-24 | 이태봉 | Method of mass-culturing inonotus obliquus and phellinus linteus using cereals or medical plants, food comprising the inonotus obliquus and the phellinus linteus cultured thereby, and method of manufacturing the food |
CN102599006A (en) * | 2012-02-25 | 2012-07-25 | 何寒 | Rapid preparation method for edible fungi grain strain |
JP2016077283A (en) * | 2014-10-13 | 2016-05-16 | 農業会社法人自然生命代替医学(株) | Fermented zen food composition comprising mixture of agricultural product and cereal mycelium, for preventing cancer and diabetes and for enhancing immunity, and production method thereof |
CN105993594A (en) * | 2016-05-24 | 2016-10-12 | 宜州市廖哥山食用菌农民专业合作社 | Method for cultivating mulberry branch auricularia polytricha with silkworm-raising room |
CN113812304A (en) * | 2021-11-05 | 2021-12-21 | 桂东森宜食用菌发展有限公司 | Edible mushroom inoculation method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104956914A (en) | Breeding method for natural ganoderma lucidum | |
JP5756477B2 (en) | Kumestrol production method | |
CN110122193B (en) | Cordyceps militaris cultivation method capable of stabilizing high-content polysaccharide | |
KR100823541B1 (en) | Mushroom cultivation method | |
KR20150024629A (en) | Medium for cultivating mushrooms comprising medicinal herb and preparation method thereof | |
CN106431571A (en) | Ganoderma lucidum karst cultivation method for improving ganoderma lucidum polysaccharides and selenium content | |
JP2012060974A (en) | Method for artificially culturing mushroom, and method for producing ganoderenic acid compounds | |
CN104429622A (en) | Method for producing shiitake mushroom mycelia by using ginkgo leaves | |
JP2001017158A (en) | Culture medium composition for culturing phellinus linteus mycelium and culture of phellinus linteus mycelium using the same composition | |
CN104855137B (en) | Method for increasing yield of antrodia cinnamomea and bioactive substance content of antrodia cinnamomea | |
CN107873391B (en) | Fungus inhibiting culture medium for cultivating agaric and cultivation method thereof | |
KR100889927B1 (en) | Method of mass-culturing inonotus obliquus and/or phellinus linteus using silkworm, food comprising the inonotus obliquus and/or phellinus linteus cultured thereby, and method of manufacturing the food | |
CN112442449B (en) | Ramaria original strain culture medium and application thereof as well as Ramaria original strain and culture method thereof | |
CN107027516A (en) | A kind of selenium-rich Periostracum cicadae, its cultural method and application | |
CN108812070A (en) | A kind of grifola frondosus culture substrate | |
KR100218897B1 (en) | Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium | |
KR100759021B1 (en) | Method growing edible mushroom | |
JP6496959B2 (en) | Ophiocordiceps genus fruit body formation medium and method of forming Ophiocordyceps genus fruit body | |
JP2001269164A (en) | Deer antler-shaped fruit body of ganoderma lucidum, and method for producing the same | |
KR100204984B1 (en) | The method of culturing phellinus linteus | |
JP3665845B2 (en) | Artificial culture method of Kyushu insecticide | |
KR101042159B1 (en) | Functional foods made of sparassis crispas and the method of making it | |
KR102117227B1 (en) | a phellinus linteus cultivating method using Cudrania tricuspidata and the phellinus linteus | |
KR20220078074A (en) | Cultivation Method of Microbial Culture of Cordyceps sp. Comprising Vitamin D, and Microbial Culture Using the Same | |
CN105622256A (en) | Cultivation method of ganoderma lucidum antler substitute material |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20060905 |