JP6496959B2 - Ophiocordiceps genus fruit body formation medium and method of forming Ophiocordyceps genus fruit body - Google Patents

Description

  The present invention relates to a medium for forming an Ophiocordyceps fruit body and a method for forming an Ophiocordyceps fruit body.

  2. Description of the Related Art Conventionally, various health foods have been used to ingest nutritional components that cannot be covered by normal eating habits in order to spend a more healthy daily life.

  Among them, Cordyceps has been prized since ancient times as a material for herbal medicine and medicinal herbs, and health foods made by processing Cordyceps in a prescribed dosage form are increasingly used as useful for improving lung health and immunity in recent years. Attention has been paid.

  Cordyceps is a stalk and rod-shaped fruit body (coccus) formed by Ophiocordyceps sinensis, a member of the Aspergillus ergot family, parasitic on insects.

  Accordingly, Cordyceps is naturally expressed and harvested in areas where host insects are present.

  Moreover, Ophiocordyceps sobolifera, which belongs to the Aspergillus ergotaceae family, similar to Cordyceps sinensis, is a fungus that parasitizes and forms a fruiting body. reference.).

  Semi-bamboo contains a lot of cordycepin in its fruiting bodies, and it has been gradually revealed by various studies that it exhibits an excellent effect on nutritional tonic.

  Therefore, semi-bamboo is attracting attention as an anti-aging alternative to traditional caterpillars and a food and drug for health promotion.

JP 2009-286746 A

  As described above, Cordyceps sinensis and semi-bamboo are highly useful materials as food materials and pharmaceutical raw materials, but there is still room for development of a culture medium and production technology for efficient mass production.

  That is, it is possible to form a fruiting body of the genus Ophiocordyceps relatively efficiently if it is a medium prepared using a medium reagent in a laboratory or the like, but it is not suitable for mass production.

  The present invention has been made in view of such circumstances, and provides a medium for forming an Ophiocordyceps genus fruit body capable of forming a fruit body of the genus Ophiocordyceps relatively inexpensively and efficiently.

  The present invention also provides a method for forming an Ophiocordyceps genus fruit body that can efficiently form a fruit body of the genus Ophiocordyceps at a relatively low cost.

  In order to solve the above conventional problems, in the Ophiocordyceps fruiting body formation medium according to the present invention, (1) 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed. The moisture was adjusted to 50 to 70% by weight.

  Moreover, the culture medium for Ophiocordyceps fruiting body formation which concerns on this invention has the characteristics also in (2) Furthermore adding the leek extract.

  In addition, according to the method for forming an Ophiocordyceps fruiting body according to the present invention, (3) 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed and moisture is 50 to 70 parts by weight. Inoculation step of inoculating Ophiocordyceps genus in a medium for Ophiocordyceps fruiting body formation adjusted to%, and culture in which the culture medium for Ophiocordyceps fruiting body that has undergone the above inoculation process is kept at 20 to 25 ° C. And having a process.

In addition, the method for forming an Ophiocordyceps genus fruit body according to the present invention is also characterized by the following points.
(4) A leek extract should be added to the fruiting body formation medium.
(5) The culturing step includes an initial culturing step, an aging culturing step, and a fruit body formation culturing step. In the initial culturing step, a medium for forming an Ophiocordyceps genus fruit body that has undergone the inoculation step is 20 to 25. Culturing in the dark at ℃, and in the aging culture step, the medium for forming the Ophiocordyceps fruiting body that has undergone the initial culturing step is at a temperature of 20 to 25 ℃ in a cycle of 12 hours in the dark and 12 hours in the dark. Culturing under conditions, the fruiting body formation culture step is to form fruiting bodies while culturing the Ophiocordyceps genus fruiting body formation medium that has undergone the aging culture process under a temperature condition of 20 to 25 ° C.
(6) Before the fruiting body formation culture step, a voltage of 10 to 60 kV is applied in a pulse form from 1 to 250 nsec to the Ophiocordyceps fruiting body formation medium.
(7) In the fruiting body formation culture step, culture is performed in a 400-600 hPa atmospheric pressure atmosphere.

  According to the Ophiocordyceps fruiting body formation medium according to the present invention, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara were mixed to adjust the water content to 50 to 70% by weight. It is possible to provide a medium for forming an Ophiocordyceps fruiting body that can form a fruiting body of the genus Ophiocordyceps relatively inexpensively and efficiently.

  Furthermore, if a leek extract is added, the yield of the fruiting body of the genus Ophiocordyceps can be improved, and the cordycepin content of the fruiting body of the genus Ophiocordyceps can be increased.

  Further, according to the method for forming an Ophiocordyceps fruiting body according to the present invention, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed to adjust the water content to 50 to 70% by weight. An inoculation step for inoculating the genus Ophiocordyceps in the Ophiocordyceps genus body-forming medium, and a culture step for culturing while maintaining the Ophiocordyceps genus body formation medium after the inoculation step at 20 to 25 ° C; Therefore, it is also possible to provide a method for forming an Ophiocordyceps genus fruit body that can form an Ophiocordyceps genus fruit body relatively inexpensively and efficiently.

  In addition, if the leek extract is added to the fruiting body formation medium, the yield of the fruiting body of the genus Ophiocordyceps can be further improved, and the cordycepin content of the fruiting body of the genus Ophiocordyceps can be increased.

  The culturing step includes an initial culturing step, an aging culturing step, and a fruit body formation culturing step. In the initial culturing step, a medium for forming an Ophiocordyceps genus fruit body that has undergone the inoculation step is 20 to 25 ° C. In the aging culture step, the Ophiocordyceps fruiting body formation medium that has undergone the initial culturing step is subjected to a temperature condition of 20 to 25 ° C. in a cycle of 12 hours in the dark and 12 hours in the dark. If the fruiting body formation culture step is to form the fruiting body while culturing the Ophiocordyceps fruiting body formation medium that has undergone the aging culture process under a temperature condition of 20 to 25 ° C. Therefore, the fruiting body of the genus Ophiocordyceps can be cultured more firmly and stably.

  In addition, if a voltage of 10 to 60 kV is applied in a pulse form of 1 to 250 nsec to the Ophiocordyceps fruiting body formation medium before the fruiting body formation culture step, the formation of the Ophiocordyceps fruiting body is further promoted. Can do.

  Moreover, in the said fruit body formation culture | cultivation process, if it culture | cultivates in 400-600 hPa atmospheric pressure atmosphere, formation of an Ophiocordyceps genus fruit body can be promoted more.

It is explanatory drawing which showed the difference in the yield by a high voltage pulse application. It is explanatory drawing which showed the difference in the cordycepin content by high voltage pulse application. It is explanatory drawing which showed the difference in the yield by culture | cultivation in a pressure-reduced atmosphere. It is explanatory drawing which showed the difference in the cordycepin content by culture | cultivation in a pressure-reduced atmosphere.

  The present invention provides an Ophiocordyceps fruiting body-forming medium capable of forming a fruiting body of the genus Ophiocordyceps relatively inexpensively and efficiently.

  In particular, in the Ophiocordyceps fruit body formation medium according to this embodiment, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed to adjust the water content to 50 to 70% by weight. It has the feature in that.

  Pressure-biased soybeans are those obtained by heating soybeans by steam or roasting to make pressure-biased (flakes), and are widely used in feeds and the like.

  Pressure-biased soybeans have a flatter shape than normal soybeans, and many cracks are formed at the edges, so they absorb water quickly and swell, and the moisture in the medium is kept as constant as possible. It also has the role of a water retentive body to keep the

  Dried okara is dried okara that is produced as a by-product when producing tofu or soy milk, and has a water content of about 2 to 8% and is widely marketed for food and feed.

  Dried okara contains a large amount of water-insoluble fiber derived from soybean and is extremely useful as a sugar source of the genus Ophiocordyceps.

  In the Ophiocordyceps fruit body formation medium according to this embodiment, these pressure-biased soybeans and dried okara are mixed at a ratio of 8 to 9.5 parts by weight: 0.5 to 2 parts by weight, and the water content is 50 to 70 parts by weight. % Is adjusted.

  When the weight ratio of the pressure-biased soybean is less than 8 parts by weight (the weight ratio from the dried okara is more than 2 parts by weight), the air gap is formed by filling the voids formed between the pressure-biased soybeans with the okara. It is not preferable.

  Moreover, it is not preferable that the weight ratio of the pressure-biased soybean exceeds 9.5 parts by weight (the weight ratio from the dried okara is less than 0.5 parts by weight) because the okara does not function as a binder for the pressure-biased soybeans.

  On the other hand, when the water content is less than 50% by weight or more than 70% by weight, the shape retention as a medium for forming a semi-bamboo fruit body deteriorates and adversely affects the growth of mycelia of the genus Ophiocordyceps.

  Ophiocordyceps fruiting bodies can be efficiently formed by mixing pressure-biased soybeans and dried okara in a proportion of 8 to 9.5 parts by weight: 0.5 to 2 parts by weight and adjusting the water content to 50 to 70% by weight. Medium.

  In addition, a leek extract may be added to the Ophiocordyceps genus fruit formation medium as an ingredient for promoting Ophiocordyceps genus fruit formation.

  Specifically, it can be boiled in water at a rate of 1 L per 100 g of leek, and the resulting broth can be added as a leek extract to the aforementioned Ophiocordyceps fruiting body formation medium, and by drying this broth The obtained dried product may be added as a leek extract.

  By adopting such a configuration, formation of a fruiting body of the genus Ophiocordyceps can be further promoted, and the amount of cordycepin contained in the fruiting body can be further increased.

  The present invention also provides a method for forming an Ophiocordyceps genus fruit body, which is capable of forming an Ophiocordyceps genus fruit body relatively inexpensively and efficiently.

  In particular, in the method for forming an Ophiocordyceps fruiting body according to this embodiment, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed to adjust the water content to 50 to 70% by weight. An inoculation step of inoculating the genus Ophiocordyceps in the Ophiocordyceps genus body formation medium, and a culture step of culturing while maintaining the Ophiocordyceps genus body formation medium that has undergone the inoculation step at 20 to 25 ° C. It has the feature in having.

Here, in the inoculation step, the aforementioned ascomycete fungi belonging to the genus Ophiocordyceps are added to the aforementioned Ophiocordyceps genus body-forming medium according to the present embodiment, that is, Ophiocordyceps sinensis (so-called Cordyceps) or Ophiocordyceps sobolifera (so-called semi-bamboo). Inoculate. The amount of the fungus to be inoculated is not particularly limited, but it is preferable to inoculate about 1.0 × 10 ⁴ cfu or more of mycelia per 1 kg of the Ophiocordyceps fruiting body formation medium. If it is less than 1.0 × 10 ⁴ cfu per kg, it is not preferable because it takes time for the mycelium to sufficiently spread to the medium. Further, the upper limit amount is not particularly limited, but can be set to, for example, 1.0 × 10 ⁶ cfu if a standard is described from the viewpoint of production efficiency.

  In the culturing step, the fruiting body is formed by culturing the Ophiocordyceps genus fruiting body formation medium that has undergone the inoculation process while maintaining the temperature at 20 to 25 ° C.

  In addition, in the method for forming an Ophiocordyceps fruiting body according to this embodiment, a leek extract may be added to the fruiting body formation medium. As described above, the leek extract may be in a liquid form or may be dispersed in the medium as a powder form.

  The culturing step includes an initial culturing step, an aging culturing step, and a fruit body formation culturing step. In the initial culturing step, a medium for forming an Ophiocordyceps genus fruit body that has undergone the inoculation step is 20 to 25 ° C. In the aging culture step, the Ophiocordyceps fruit body formation medium that has undergone the initial culturing step is subjected to a temperature condition of 20 to 25 ° C. in a cycle of 12 hours in the dark and 12 hours in the dark The fruiting body formation culture step may be performed by forming the fruiting body while culturing the Ophiocordyceps genus fruiting body formation medium that has undergone the aging culture process under a temperature condition of 20 to 25 ° C. .

  The initial culturing step is a step for sufficiently allowing the mycelium to propagate on the surface and inside of the Ophiocordyceps fruiting body formation medium, and culturing is performed in the dark at 20 to 25 ° C. The culture time is not particularly limited, but is about 30 to 50 days when the culture is performed with the above-mentioned inoculation amount. That is, the time until the hyphae sufficiently propagate varies depending on the amount of the Ophiocordyceps fruiting body formation medium used.

  The ripening culture process is a culture process for promoting reproductive growth of sufficiently propagated mycelium of the genus Ophiocordyceps. Specifically, by culturing under a temperature condition of 20 to 25 ° C. in a cycle of 12 hours in a light place and 12 hours in a dark place, formation of primordia is promoted in the mycelium of the genus Ophiocordyceps. Although the culture time is not particularly limited, it is about 10 to 20 days when the culture is performed with the above inoculum amount. That is, as in the initial culturing step, the time until the mycelium sufficiently reproduces and grows varies depending on the amount of the Ophiocordyceps genus body-forming medium used.

  The fruiting body formation culture process is a culture process for causing the fruiting body formation of the genus Ophiocordyceps. By performing this fruiting body formation culture step, it is possible to cause elongation and growth of the fruiting body of the genus Ophiocordyceps.

  And it is preferable to harvest the Ophiocordyceps genus fruit body extended by performing this fruit body formation culture | cultivation process in about 5-7 cm length. The Ophiocordyceps genus with this length can obtain a sufficient yield while maintaining useful ingredients.

  In addition, in the method for forming an Ophiocordyceps genus fruit body according to the present embodiment, a voltage of 10 kV to 60 kV is applied in a pulse shape from 1 ksec to 250 ksec to the Ophiocordyceps genus fruit body formation medium before the fruit body formation culture step. Anyway.

  Applying a voltage of 10 kV to 60 kV, more preferably a voltage of 20 kV to 50 kV, to the Ophiocordyceps spp. In addition, the cordycepin content contained in the fruit body can be increased.

  Further, in the method for forming an Ophiocordyceps genus fruit body according to the present embodiment, in the fruit body formation culture step, the culture may be performed in a 400-600 hPa atmospheric pressure atmosphere. By performing such a culture, the formation of the fruiting body of the genus Ophiocordyceps can be improved.

  Hereinafter, the Ophiocordyceps genus body formation medium and the Ophiocordyceps genus body formation method using the Ophiocordyceps genus body formation medium according to the present embodiment will be described more specifically with reference to actual production examples. In the following description, an example using Ophiocordyceps sobolifera (so-called semi-bamboo) as a basidiomycete belonging to the genus Ophiocordyceps is mentioned, but the present invention is not limited to this. However, it does not prevent the applicant from limiting basidiomycetes belonging to the genus Ophiocordyceps to Ophiocordyceps sobolifera.

[1. Preparation of semi-bamboo fruit body formation medium]
First, preparation of a semi-bamboo fruit body formation medium will be described. Here, five types of fruit body formation media A1 to E1 were prepared in which the mixing ratio of the pressure-biased soybean and dried okara was 7: 3 to 9.5: 0.5 and the water content was adjusted to 50 to 70%. In addition, as a comparative test medium, a comparative medium P1 and a comparative medium Q1 in which the mixing ratio of pressure-biased soybeans and dried okara deviates up and down from the above range while the water content is 60%, Comparative medium R1 in which the mixing ratio of uneven soybean and dried okara is within the range of 7: 3 to 9.5: 0.5 (specifically, 9: 1), but the water content deviates up and down from the above range. A comparative medium S1 was also prepared.

As a specific method for preparing each medium, first, pressure-biased soybeans and dried okara were weighed and mixed as shown in Table 1, and water was added and kneaded.

  Next, 2.5 kg of each prepared medium is placed in a mushroom culture bag manufactured by F-Tech Co., Ltd., formed into a cube of approximately 10 cm x 20 cm x 20 cm, autoclaved at 121 ° C for 30 minutes, and then allowed to cool. Thus, a sterilized medium was obtained. In Table 1, one with a total weight of less than 2.5 kg was prepared by adding one more batch.

  Among the media thus prepared, the properties of the fruiting body formation media A1 to E1 were observed, and any of the fruiting body formation media A1 to E1 has an appropriate shape retaining property, It was confirmed that moderate voids were formed in the medium.

  On the other hand, when the comparative medium P1 was observed, the amount of okara that functions as a binder was small with respect to the amount of pressure-biased soybeans, and the above-described cubic shape was collapsed, and the shape retention was difficult.

  In addition, when the comparative culture medium Q1 was observed, the amount of okara that functions as a linkage was too large, so that although it has shape retention, voids in the culture medium have been filled with okara, from the viewpoint of air permeability. Had a problem.

  Further, when the comparative medium R1 was observed, there was a problem in shape retention because the water content was too small.

  Moreover, when the comparative culture medium S1 was observed, it was extremely poor in handling due to too much water, and the shape retention was also deteriorated.

  From these results, the fruit body formation media A1 to E1 according to the present embodiment are prepared such that the mixing ratio of pressure-biased soybeans and dried okara is 7: 3 to 9.5: 0.5 and the water content is 50 to 70%. Therefore, it was shown that it can function as a medium for forming a good semi-bamboo fruit body.

[2. (Inoculation process)
Next, semi-bamboo (Ophiocordyceps sobolifera) was inoculated on the sterilized fruit body formation media A1 to E1 and comparative media P1, Q1, R1, and S1. Specifically, 50 ml of a bacterial solution containing 5 × 10 ³ cfu / ml of spores was sprayed on each prepared medium for inoculation.

[3. (Initial culture process)
Next, initial culture was performed on the fruiting body formation mediums A1 to E1 and the comparative mediums P1, Q1, R1, and S1 that had been inoculated. Specifically, the culture room whose temperature was adjusted to 20 to 25 ° C. was darkened and cultured for 30 days.

[4. Aging culture process)
Next, ripening culture was performed on the fruit body formation media A1 to E1 and the comparison media P1, Q1, R1, and S1 that had undergone the initial culture process. Specifically, in a culture room temperature-controlled at 20 to 25 ° C., the lighting device was set to a cycle that was turned on for 12 hours and then turned off for 12 hours, and cultured for 10 days.

[5. Fruiting body formation culture process)
Next, fruit body formation culture was performed on the fruit body formation media A1 to E1 and the comparison media P1, Q1, R1, and S1 that had undergone the aging culture process. Specifically, the culture room whose temperature was adjusted to 20 to 25 ° C. was clarified and cultured in an atmospheric pressure atmosphere for 20 days.

[6. Harvesting process)
After performing the fruiting body formation culture step, harvesting was performed by picking up the semi-bamboo fruiting bodies formed on each medium with a fingertip. Harvesting targeted semi-bamboo fruit bodies grown to a length of 5 cm or more.

[7. (Yield comparison)
Next, the weights of fruit bodies obtained from the fruit body formation media A1 to E1 and the comparison media P1, Q1, R1, and S1 were respectively compared. The results are shown in Table 2.

  As can be seen from Table 2, all of the fruiting body formation media A1 to E1 were able to harvest more fruiting bodies compared to the comparative culture media P1, Q1, R1, and S1. In addition, the amount of medium necessary to obtain 100 g of fruiting bodies when using a potato glucose medium used for fruit body formation culture of semi-bamboo in the laboratory is 500 g, and it costs 2000 yen per kg of the same medium. However, since the fruit body formation mediums A1 to E1 are at most about 200 yen, the semibamboo fruit body formation medium according to the present embodiment can form semibamboo fruit bodies relatively inexpensively and efficiently. It was shown that there is.

[8. (Verification of differences due to the addition of leek extract)
Next, among each of the above-mentioned fruiting body formation media, by adding a leek extract to the fruiting body formation medium A1, the difference in the yield of semi-bamboo fruiting bodies and the difference in cordycepin content were verified. .

  The leek extract was prepared as follows. In other words, 1000 g of green onion cut to a length of about 5 cm is put into 10 L of water, boiled for 60 minutes to obtain boiled juice while performing hot water extraction, and 10 g of leek extract powder is obtained by freeze-drying this boiled juice. It was.

  Next, 2.5 g of leek extract was added to and uniformly dispersed in the above-mentioned fruit body formation medium A1 (2.5 kg) to prepare a fruit body formation medium A2 containing leek extract.

Next, with respect to the fruit body forming medium A1 and the fruit body forming medium A2, the above-mentioned [2. Inoculation step] to [6. Processing was performed in the same manner as in the “harvesting process” to obtain a fruit body of the semi-bamboo. The harvest weight and cordycepin content of the resulting semi-bamboo fruiting bodies were examined. The cordycepin content was measured by extracting 80 g of the resulting semi-bamboo fruit body with hot water for 2 hours, and lyophilizing the filtrate obtained by filtration to obtain a dried product. Then, the solution was filtered through a membrane filter having a pore size of 0.45 μm and then subjected to HPLC. The HPLC measurement conditions were an ODS column (4.5 mm × 150 mm), the moving bed was methanol: KH ₂ PO ₄ buffer = 15: 85, the flow rate was 1 ml / min, and the detection wavelength was 254 nm. The results are shown in Table 3.

  As can be seen from FIG. 3, the medium for forming semi-bamboo fruit bodies according to the present embodiment can increase the yield of semi-bamboo fruit bodies by further adding leek extract, and is contained per unit amount of fruit bodies. Can increase the amount of cordycepin.

  Although specific experimental results are omitted, according to the study of the present inventor, the amount of leek extract added to the medium is most preferably 2.5 g per 2.5 kg of medium, It is possible to improve the cordycepin content. If the amount is less than 1 g, these effects are hardly exhibited, and even if added in excess of 5 g, the effect is not enhanced, and the yield and the cordycepin content are lowered, which is not preferable.

[9. (Verification of differences due to high voltage pulse application)
Next, a high voltage pulse was applied to the above-mentioned fruit body formation medium A1 to perform a voltage application process, thereby verifying the difference in the yield of semi-bamboo fruit bodies and the difference in cordycepin content.

  Specifically, the fruit body formation medium A1 (100 g) was placed in two 500 ml polypropylene containers, respectively, sterilized at 120 ° C. for 30 minutes and allowed to cool, and then the above-mentioned [2. Inoculation step] to [4. Treatment was performed in the same manner as in the aging culture step. It was confirmed that the mycelium was sufficiently propagated in the medium in each container that had undergone the aging culture process, and that a primordium was formed by microscopic examination.

  Next, using a high voltage pulsed power supply (Weve Motion Gun: TYC-1) manufactured by Toshinshin Co., Ltd., a pulse of 10 kV to 60 kV is applied to one bottle of fruit body formation medium A1 for 100 nsec. The application process was performed. In the following description, the fruit body forming medium A1 that has undergone the voltage application step is referred to as a fruit body forming medium A1v by adding “v” to each symbol.

  And about the fruiting body formation culture medium A1 which has not passed through the voltage application process, and the above-mentioned [5. Fruiting body formation culture step] and [6. Harvesting process] was performed to obtain semi-bamboo fruit bodies. The harvest weight of the resulting semi-bamboo fruiting body is shown in FIG.

  As can be seen from FIG. 1, it was revealed that the generation amount of semi-bamboo fruit bodies reached a maximum of 32 g by electrical pulse stimulation, and the generation amount of fruit bodies was significantly increased as compared with 12 g without treatment.

  Also, the effect was different depending on the applied voltage, and the best result was obtained at 30 kV. Compared to no treatment, the occurrence rate (BE: Biological Efficiency) was 267% in the applied zone.

  Next, quantification was performed on cordycepin. As shown in FIG. 2, the content of cordycepin was remarkably increased, and a remarkable effect was observed when 30 kV was applied as in the case of the fruiting body generation amount.

  On the other hand, in the untreated group, it was found to be 42 mg per gram of completely dry fruiting body, while it was found to increase significantly to 88 mg in the applied group. Cordycepin, also called 3'-deoxyadenosine, is a structure that has lost the hydroxyl group (OH group) from the 3 'position of one adenosine of the nucleoside. As expected functionality, it is said to have an inhibitory effect on DNA and RNA synthesis. Therefore, it is considered to have an effect of suppressing the growth of malignant cells (Kredich and Guarino, 1961).

  As described above, it has been confirmed that the content of cordycepin, which is a metabolite, is greatly increased and the generation amount of fruiting bodies is increased through the voltage application step.

[10. (Verification of differences due to vacuum culture)
Next, about the difference in the yield of semi-bamboo fruit bodies and the difference in the content of cordycepin by culturing the above-mentioned fruit body formation medium A1 in an atmosphere of 400 to 600 hPa in the fruit body formation culture step. Verification was performed.

  Specifically, the fruit body formation medium A1 (100 g) was placed in two 500 ml polypropylene containers, respectively, sterilized at 120 ° C. for 30 minutes and allowed to cool, and then the above-mentioned [2. Inoculation step] to [4. Treatment was performed in the same manner as in the aging culture step. It was confirmed that the mycelium was sufficiently propagated in the medium in each container that had undergone the aging culture process, and that a primordium was formed by microscopic examination.

  Subsequently, one bottle of the produced fruit body formation medium A1 was placed in a constant temperature incubator whose pressure was adjusted to 400 to 600 hPa to perform a fruit body formation culture process. In the following description, the fruit body formation medium A1 cultured in a fruit body formation culture step in a 400-600 hPa atmospheric pressure is marked with “d” to each fruit body formation medium. It is called A1d.

  The fruiting body formation medium A1 that has not been exposed to the reduced pressure atmosphere and the fruiting body formation medium A1d that has been exposed to the reduced pressure atmosphere are described in [6. Harvesting process] was performed to obtain semi-bamboo fruit bodies. The harvest weight of the obtained semi-bamboo fruit body is shown in FIG.

  As can be seen from FIG. 3, the maximum amount of semi-bamboo fruit bodies generated by exposure to a reduced pressure atmosphere was 30 g, which was found to be significantly increased compared to 12 g of untreated.

  In addition, there is a difference in effect due to the difference in pressure.At pressures exceeding 600 hPa, the effect of increasing the yield of semi-bamboo fruit bodies is not significant, and when the pressure is reduced to less than 400 hPa, the yield of semi-bamboo fruit fruit bodies decreases rather than significantly. Indicated.

  Next, quantification was performed on cordycepin. As shown in FIG. 4, the content of cordycepin was remarkably increased, and a remarkable effect was recognized under a 400 to 600 hPa atmospheric pressure as in the case of the fruit body generation amount.

  As described above, in the medium for forming an Ophiocordyceps fruiting body according to the present embodiment, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed and moisture is 50 to 70 parts by weight. Therefore, it is possible to provide a medium for forming an Ophiocordyceps fruiting body capable of forming a fruiting body of the genus Ophiocordyceps relatively inexpensively and efficiently.

  Further, according to the method for forming an Ophiocordyceps fruiting body according to the present embodiment, 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara are mixed to bring the moisture to 50 to 70% by weight. An inoculation step of inoculating the genus Ophiocordyceps in an adjusted Ophiocordyceps genus body-forming medium, and a culture step of culturing while maintaining the Ophiocordyceps genus body formation medium after the inoculation step at 20 to 25 ° C; Therefore, it is also possible to provide a method for forming an Ophiocordyceps genus fruit body that can efficiently form a fruit body of the genus Ophiocordyceps at a relatively low cost.

  Finally, the description of each embodiment described above is an example of the present invention, and the present invention is not limited to the above-described embodiment. For this reason, it is a matter of course that various modifications can be made in accordance with the design and the like as long as they do not depart from the technical idea according to the present invention other than the embodiments described above.

Claims (7)

  A medium for forming an Ophiocordyceps fruiting body by mixing 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara and adjusting the water content to 50 to 70% by weight.   Furthermore, the leek extract is added, The culture medium for Ophiocordyceps genus fruit body formation of Claim 1 characterized by the above-mentioned. Inoculate Ophiocordyceps genus in a medium for the formation of Ophiocordyceps fruiting bodies by mixing 8 to 9.5 parts by weight of pressure-biased soybeans and 0.5 to 2 parts by weight of dried okara and adjusting the water content to 50 to 70% by weight. An inoculation process;
A culture step of culturing while maintaining the Ophiocordyceps fruiting body formation medium that has undergone the inoculation step at 20 to 25 ° C;
Of forming an Ophiocordyceps fruiting body having   The method for forming an Ophiocordyceps genus fruit body according to claim 3, wherein a leek extract is added to the fruit body formation medium. The culture process comprises an initial culture process, an aging culture process, and a fruit body formation culture process,
In the initial culture step, the Ophiocordyceps fruiting body formation medium that has undergone the inoculation step is cultured in the dark at 20 to 25 ° C.,
In the ripening culture step, the Ophiocordyceps fruiting body formation medium that has undergone the initial culturing step is cultured under a temperature condition of 20 to 25 ° C. in a cycle of 12 hours in the dark and 12 hours in the dark,
The said fruit body formation culture process forms a fruit body, cultivating the Ophiocordyceps genus fruit body formation culture medium which passed through the said ripening culture process on 20-25 degreeC temperature conditions, It is characterized by the above-mentioned. The formation method of the Ophiocordyceps genus fruit body of Claim 4.   6. The Ophiocordyceps genus body according to claim 5, wherein a voltage of 10 to 60 kV is applied in a pulse form from 1 nsec to 250 nsec to the Ophiocordyceps genus body formation medium before the fruiting body formation culture step. Forming method.   The method for forming an Ophiocordyceps genus fruit body according to claim 5 or 6, wherein in the fruit body formation culture step, culturing is performed in a pressure atmosphere of 400 to 600 hPa.