TWI601818B - Medium of solid fermentation for inonotus obliquus and preparation method thereof - Google Patents

Medium of solid fermentation for inonotus obliquus and preparation method thereof Download PDF

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TWI601818B
TWI601818B TW105144201A TW105144201A TWI601818B TW I601818 B TWI601818 B TW I601818B TW 105144201 A TW105144201 A TW 105144201A TW 105144201 A TW105144201 A TW 105144201A TW I601818 B TWI601818 B TW I601818B
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王伯綸
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天明製藥股份有限公司
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Description

白樺茸的培養基及其製備方法 Chaga culture medium and preparation method thereof

本發明係提供一種白樺茸的培養基及其製備方法，尤指一種可縮短白樺茸產物採收時間之培養基及其製備方法 The present invention provides a Chaga culture medium and a preparation method thereof, especially a medium capable of shortening the harvest time of Chaga products and a preparation method thereof

白樺茸(Inonotus obliquus)是一種民間藥用真菌，主要分佈於俄羅斯及東歐地區。研究顯示白樺茸具有預防乳腺癌、肝癌、子宮癌、胃癌、糖尿病及高血壓的效果，近年來，其營養和藥用價值逐漸引起人民的關注，成為醫藥工業和保健品工業的熱門開發領域。 Inonotus obliquus is a folk medicinal fungus mainly distributed in Russia and Eastern Europe. Studies have shown that Chaga has the effect of preventing breast cancer, liver cancer, uterine cancer, stomach cancer, diabetes and hypertension. In recent years, its nutritional and medicinal value has gradually attracted people's attention, and has become a hot development field in the pharmaceutical industry and health care products industry.

目前野生的白樺茸價格昂貴，且來源日益枯竭，很難滿足市場的需求。使用人工栽培如固態發酵培養技術，由於現階段栽培困難，產量較低。現有白樺茸之固態培養基，多以木屑和麥麩搭配其他營養物質製備培養基，樺木中雖含有豐富的營養物質使得養效果較好，但是，並無法有效提高白樺茸菌絲體產物的產量。 Wild Chaga is currently expensive and the source is increasingly exhausted, making it difficult to meet market demand. Artificial cultivation, such as solid-state fermentation culture technology, is used because of the difficulty in cultivation at this stage, and the yield is low. The existing solid medium of Chaga is mostly prepared by using wood chips and wheat bran with other nutrients. Although birch is rich in nutrients, the effect is better, but it can not effectively increase the yield of the birch mycelium products.

本發明係鑑於前述習知技術之課題者，其目的在於提供一種新穎的白樺茸的培養基以及其製備方法，在藉由固態發酵模擬白樺茸天然的生長環境，並維持產物營養成份的同時，亦能有效率的促進白樺茸吸收 營養、提高生長速度，進而縮短採收菌絲體產物的時間，並有效提升產量。 The present invention has been made in view of the above-mentioned problems of the prior art, and an object thereof is to provide a novel Chaga culture medium and a preparation method thereof, which are capable of simulating the natural growth environment of Chaga by solid-state fermentation and maintaining the nutritional content of the product. Can effectively promote the absorption of Chaga Nutrition, increase growth rate, and thus shorten the time of harvesting mycelium products, and effectively increase production.

有鑑於前述之問題與缺失，發明人以多年之經驗累積，並發揮想像力與創造力，在不斷試作與修改之後，始有本發明之一種可縮短白樺茸產物採收時間之培養基配方。 In view of the aforementioned problems and deficiencies, the inventors have accumulated years of experience, and exerted imagination and creativity. After continuous trial and modification, the present invention has a medium formula which can shorten the harvesting time of the birch product.

本發明之主要目的係提供一種可縮短白樺茸採收時間之培養基，其至少包括：一乾料部分，其重量百分比濃度為40%，其中該乾料部分包含：重量百分濃度約為30%~40%之樺樹皮；重量百分濃度為5%~15%之綠豆粉；重量百分濃度為1%~5%之葡萄醣；重量百分濃度為0.05%~1%之磷酸二氫鉀；重量百分濃度為0.05%~1%之硫酸鎂；重量百分濃度為20%~50%之中草藥組合物；以及一含膠體水溶液，其重量百分比濃度為60%，並且含有體積百分濃度為1%~5%的瓊脂。 The main object of the present invention is to provide a medium capable of shortening the time of harvesting of Chaga, comprising at least: a dry portion having a concentration by weight of 40%, wherein the dry portion comprises: a concentration of about 30% by weight. 40% birch bark; 5% to 15% by weight of mung bean powder; 1% to 5% by weight of glucose; weight percent concentration of 0.05% to 1% potassium dihydrogen phosphate; weight a percentage concentration of 0.05% to 1% of magnesium sulfate; a concentration by weight of 20% to 50% of the herbal composition; and a colloidal aqueous solution having a concentration of 60% by weight and containing a volume percent concentration of 1 %~5% agar.

根據本發明之一較佳實施態樣，其中該中草藥組合物係選自一種由以下成份所組成之群組或其組合物：黃耆、紫草、生薑、山藥、玄明粉。 According to a preferred embodiment of the present invention, the Chinese herbal medicine composition is selected from the group consisting of the following components or a combination thereof: Astragalus, Lithospermum, Ginger, Yam, and Xuan Ming Powder.

根據本發明之一較佳實施態樣，其中該樺樹皮呈粉末狀，係由樺木屬植物之樹皮經乾燥與研磨所製備者。 According to a preferred embodiment of the present invention, the birch bark is in the form of a powder prepared by drying and grinding the bark of a birch plant.

根據本發明之一較佳實施態樣，其中該培養基之酸鹼值介於約6至7。 According to a preferred embodiment of the invention, the medium has a pH of between about 6 and 7.

根據本發明之一較佳實施態樣，其中該樺樹皮之重量百分濃度約為30%~40%；該綠豆粉之重量百分濃度為5%~15%；該葡萄醣之重量 百分濃度為1%~5%；該磷酸二氫鉀之重量百分濃度為0.05%~1%；該硫酸鎂之重量百分濃度為0.05%~1%；該中草藥組合物之重量百分濃度為20%~50%；以及該含膠體水溶液具有體積百分濃度為1%~5%的瓊脂。 According to a preferred embodiment of the present invention, the weight percentage of the birch bark is about 30% to 40%; the weight concentration of the mung bean powder is 5% to 15%; the weight of the glucose The percentage concentration is from 1% to 5%; the weight percentage concentration of the potassium dihydrogen phosphate is 0.05% to 1%; the weight percentage of the magnesium sulfate is 0.05% to 1%; the weight percentage of the Chinese herbal medicine composition The concentration is 20% to 50%; and the colloidal aqueous solution has a volume percentage of 1% to 5% agar.

根據本發明之一較佳實施態樣，其中該樺樹皮之重量百分濃度約為40%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為43%；以及該含膠體水溶液具有體積百分濃度為4.6%的瓊脂。 According to a preferred embodiment of the present invention, wherein the birch bark has a weight percent concentration of about 40%; the mung bean powder has a weight percent concentration of 12.25%; the glucose has a weight percent concentration of 2.75%; The weight percent concentration of potassium dihydrogenate is 1%; the weight percent concentration of the magnesium sulfate is 1%; the weight concentration of the herbal composition is 43%; and the aqueous colloidal solution has a volume percent concentration of 4.6%. Agar.

根據本發明之一較佳實施態樣，其中該中草藥組合物係由重量百分濃度25~40%的黃耆、20~35%的紫草、10%~30%的生薑、10%~30%的山藥以及5%~15%的玄明粉所組成。 According to a preferred embodiment of the present invention, the Chinese herbal medicine composition is composed of 25 to 40% by weight of scutellaria, 20 to 35% of comfrey, 10% to 30% of ginger, and 10%. 30% yam and 5%~15% Xuanming powder.

根據本發明之一較佳實施態樣，其中該中草藥組合物係由重量百分濃度30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 According to a preferred embodiment of the present invention, the Chinese herbal medicine composition is 30% by weight of jaundice, 30% comfrey, 15% ginger, 15% yam and 10% xiaoming powder. Composed of.

本發明另提供了一種可縮短白樺茸採收時間之培養基的製備方法，其步驟包括：(A)製備乾料部分，其係依下列各組份預先混合：其重量百分濃度約為30%~40%之乾燥樺樹皮；重量百分濃度為5%~15%之綠豆粉；重量百分濃度為1%~5%之葡萄醣；重量百分濃度為0.05%~1%之磷酸二氫鉀；重量百分濃度為0.05%~1%之硫酸鎂；以及重量百分濃度為20%~50%之中草藥組合物；(B)將乾料部分滅菌處理；(C)製備含膠體水溶液，其中該含膠體水溶液含有體積百分濃度為1%~5%的瓊脂，並且該水溶液之總重量為該乾料部分1.5倍，並且混合含膠體水溶液以及乾料部分以形 成一初始培養基；以及(D)將該初始培養基經滅菌處理。 The invention further provides a preparation method of a medium capable of shortening the harvesting time of the chamomile, the steps comprising: (A) preparing a dry material portion which is pre-mixed according to the following components: the weight concentration is about 30% ~40% dry birch bark; 5% to 15% by weight of mung bean powder; 1% to 5% by weight of glucose; weight percent concentration of 0.05% to 1% potassium dihydrogen phosphate ; a concentration by weight of 0.05% to 1% of magnesium sulfate; and a concentration by weight of 20% to 50% of the herbal composition; (B) partially sterilizing the dry material; (C) preparing a colloidal aqueous solution, wherein The colloidal aqueous solution contains a concentration of 1% to 5% by weight of agar, and the total weight of the aqueous solution is 1.5 times that of the dry material portion, and the mixed colloidal aqueous solution and the dry material portion are mixed. Forming an initial medium; and (D) sterilizing the initial medium.

本發明係揭露一種白樺茸之固態培養基配方及其製備方法，並以白樺茸之菌絲體產物的生長速度做為指標，將培養基之成分及其含量作適當的調配，最後得出以綠豆粉做為氮源、葡萄糖作為碳源，並加入現有之中草藥成分，證實該固態培養基具有縮短白樺茸採收時間之功效。請參考圖示與下列之說明，其中之目的係為說明本發明之最佳實施例，而非用以限制本發明之範圍。藉由特定的具體實施例說明本發明之實施方式，熟習此技藝之人士可由本說明書所揭示之內容輕易地了解本發明之其他優點與功效。本發明亦可藉由其他不同的具體實施例加以施行或應用，本說明書中的各項細節亦可基於不同觀點與應用，在不悖離本發明之精神下進行各種修飾與變更。 The invention discloses a solid medium formula of Chaga velvet and a preparation method thereof, and the growth rate of the mycelial product of Chaga is used as an index, and the composition and content of the medium are appropriately formulated, and finally the green bean powder is obtained. As a nitrogen source, glucose as a carbon source, and the addition of existing herbal ingredients, it was confirmed that the solid medium has the effect of shortening the harvest time of the chaff. The description and the following description are for the purpose of illustrating the preferred embodiments of the invention Other embodiments of the present invention will be readily understood by those skilled in the art from this disclosure. The present invention may be embodied or applied in various other specific embodiments, and various modifications and changes can be made without departing from the spirit and scope of the invention.

菌絲體的培養與取得 Cultivation and acquisition of mycelium

於本實施例中，菌絲體的培養係選用白樺茸(Inonotus obliquus)菌絲體進行發酵。一般而言，醱酵方法有兩類：固態與液體深層醱酵。前者係將菌絲體接種於含有碳源或氮源之固態培養基(例如堆肥和木屑)進行培養而得到之生菌絲體或乾燥菌絲體。後者，則是使用固定組成之 液態培養基，在適當的環境下進行微生物的培養，以製造生質(Biomass)與其他的代謝產物(Metabolite)。本實施例係選擇前者方式培養白樺茸菌絲。 In the present embodiment, the mycelium culture is carried out by using Inonotus obliquus mycelium for fermentation. In general, there are two types of fermentation methods: solid and liquid deep fermentation. The former is a mycelium or a dried mycelium obtained by inoculating a mycelium in a solid medium (for example, compost and wood chips) containing a carbon source or a nitrogen source. In the latter case, microorganisms are cultured in a suitable environment using a liquid medium of a fixed composition to produce biomass (Biomass) and other metabolites (Metabolite). In this embodiment, the former method is used to culture the white birch hyphae.

實施例1-白樺茸固態培養基之製備方法 Example 1 - Preparation method of Chaga solid medium

步驟一：製備乾料部分，其係依下列各組份預先混合：其重量百分濃度約為30%~40%之乾燥樺樹皮；重量百分濃度為5%~15%之綠豆粉；重量百分濃度為1%~5%之葡萄醣；重量百分濃度為0.05%~1%之磷酸二氫鉀；重量百分濃度為0.05%~1%之硫酸鎂；以及重量百分濃度為20%~50%之中草藥組合物；步驟二：將乾料部分滅菌處理；步驟三：製備含膠體水溶液，其中該含膠體水溶液含有體積百分濃度為1%~5%的瓊脂，並且該水溶液之總重量為該乾料部分1.5倍，並且混合含膠體水溶液以及乾料部分以形成一初始培養基；以及步驟四：將該初始培養基經滅菌處理。其中，樺樹皮呈粉末狀，由樺木屬植物的樹皮經乾燥與研磨所製備。該中草藥組合物選自一種由黃耆、紫草、生薑、山藥、玄明粉所組成之群組或其組合物。該培養基的酸鹼值介於6至7之間。 Step 1: Prepare a dry material portion which is pre-mixed according to the following components: dry birch bark having a concentration by weight of about 30% to 40%; mung bean powder having a weight percentage of 5% to 15%; weight Percentage of glucose of 1% to 5%; weight percent concentration of 0.05% to 1% potassium dihydrogen phosphate; weight percent concentration of 0.05% to 1% of magnesium sulfate; and weight percent concentration of 20% ~50% Chinese herbal medicine composition; Step 2: Partially sterilizing the dry material; Step 3: Preparing a colloidal aqueous solution, wherein the colloidal aqueous solution contains a concentration of 1% to 5% by weight of agar, and the total amount of the aqueous solution The weight is 1.5 times the dry portion, and the colloidal aqueous solution and the dry portion are mixed to form an initial medium; and step 4: the initial medium is sterilized. Among them, the birch bark is powdery, and is prepared by drying and grinding the bark of the genus Birch. The Chinese herbal composition is selected from the group consisting of Astragalus, Lithosperm, Ginger, Yam, and Xuan Ming Powder or a combination thereof. The medium has a pH between 6 and 7.

實施例2-白樺茸固態培養基 Example 2 - Chaga Solid Medium

本實施例之白樺茸固態培養基的製備方法如實施例1所述，其中各組成之濃度依照以下所列進行製備，該樺樹皮之重量百分濃度約為20%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為63%；以及該含膠體水溶液具有體積百分濃度為4.6%的瓊脂。其中，中草藥組合物由重量百分比濃度為30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 The preparation method of the white birch solid medium of the present embodiment is as described in Example 1, wherein the concentration of each component is prepared according to the following list, and the weight percentage of the birch bark is about 20%; the weight percentage of the mung bean powder The concentration is 12.25%; the weight concentration of the glucose is 2.75%; the weight percentage of potassium dihydrogen phosphate is 1%; the weight percentage of the magnesium sulfate is 1%; the weight percentage of the Chinese herbal composition The concentration was 63%; and the colloidal aqueous solution had a 4.6% by volume agar. Among them, the Chinese herbal medicine composition is composed of 30% by weight of astragalus, 30% of comfrey, 15% of ginger, 15% of yam and 10% of xiaoming powder.

實施例3-白樺茸固態培養基 Example 3 - Chaga Solid Medium

本實施例之白樺茸固態培養基的製備方法如實施例1所述，其中各組成之濃度依照以下所列進行製備，該樺樹皮之重量百分濃度約為63%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為20%；以及該含膠體水溶液具有體積百分濃度為4.6%的瓊脂。其中，中草藥組合物由重量百分比濃度為30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 The preparation method of the white birch solid medium of the present embodiment is as described in Example 1, wherein the concentration of each component is prepared according to the following list, and the weight percentage of the birch bark is about 63%; the weight percentage of the mung bean powder The concentration is 12.25%; the weight concentration of the glucose is 2.75%; the weight percentage of potassium dihydrogen phosphate is 1%; the weight percentage of the magnesium sulfate is 1%; the weight percentage of the Chinese herbal composition The concentration was 20%; and the colloidal aqueous solution had a 4.6% by volume agar. Among them, the Chinese herbal medicine composition is composed of 30% by weight of astragalus, 30% of comfrey, 15% of ginger, 15% of yam and 10% of xiaoming powder.

實施例4-白樺茸固態培養基 Example 4 - Chaga Solid Medium

本實施例之白樺茸固態培養基的製備方法如實施例1所述，其中各組成之濃度依照以下所列進行製備，該樺樹皮之重量百分濃度約為83%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為20%；以及該含膠體水溶液具有體積百分濃度為4.6%的瓊脂。其中，中草藥組合物由重量百分比濃度為30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 The preparation method of the white birch solid medium of the present embodiment is as described in Example 1, wherein the concentration of each component is prepared according to the following list, and the weight percentage of the birch bark is about 83%; the weight percentage of the mung bean powder The concentration is 12.25%; the weight concentration of the glucose is 2.75%; the weight percentage of potassium dihydrogen phosphate is 1%; the weight percentage of the magnesium sulfate is 1%; the weight percentage of the Chinese herbal composition The concentration was 20%; and the colloidal aqueous solution had a 4.6% by volume agar. Among them, the Chinese herbal medicine composition is composed of 30% by weight of astragalus, 30% of comfrey, 15% of ginger, 15% of yam and 10% of xiaoming powder.

實施例5-最佳白樺茸固態培養基 Example 5 - Best Chaga Solid Medium

本實施例之白樺茸固態培養基的製備方法如實施例1所述，其中各組成之濃度依照以下所列進行製備，該樺樹皮之重量百分濃度約為40%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為43%；以及該含膠體水溶液具有 體積百分濃度為4.6%的瓊脂。其中，中草藥組合物由重量百分比濃度為30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 The preparation method of the white birch solid medium of the present embodiment is as described in Example 1, wherein the concentration of each component is prepared according to the following list, the weight percentage concentration of the birch bark is about 40%; the weight percentage of the mung bean powder The concentration is 12.25%; the weight concentration of the glucose is 2.75%; the weight percentage of potassium dihydrogen phosphate is 1%; the weight percentage of the magnesium sulfate is 1%; the weight percentage of the Chinese herbal composition a concentration of 43%; and the colloidal aqueous solution has The agar was 4.6% by volume. Among them, the Chinese herbal medicine composition is composed of 30% by weight of astragalus, 30% of comfrey, 15% of ginger, 15% of yam and 10% of xiaoming powder.

實驗例-各實施例固態培養基對於白樺茸菌絲體生長速度的影響 Experimental Example - Effect of Solid Medium on the Growth Rate of Chaga Mycelium

本實驗例係將白樺茸菌株植入至含有本發明之固態培養基之培養瓶；在菌絲體萌發初期，較怕雜菌感染，所需之氧氣交換較少、產出的二氧化碳及熱也較少，此時將溫度控制在攝氏25至30度，濕度控制在70%左右，以利菌絲體萌發生長。待菌絲體生長至直徑3公分時，雜菌的危害相對較小、所需之氧氣交換較多、產出的二氧化碳及熱也較多，此時將溫度控制在攝氏22至27度，濕度控制在50%，以利菌絲體持續生長。待菌絲體長至直徑8公分，為適合採摘之大小，可對白樺茸菌絲體產物進行採收。 In this experimental example, a Chaga strain is implanted into a culture flask containing the solid medium of the present invention; in the early stage of mycelium germination, it is less likely to be infected with bacteria, requires less oxygen exchange, and produces carbon dioxide and heat. Less, at this time, the temperature is controlled at 25 to 30 degrees Celsius, and the humidity is controlled at about 70% to facilitate the growth of mycelium. When the mycelium grows to a diameter of 3 cm, the damage of the bacteria is relatively small, the oxygen exchange required is more, and the carbon dioxide and heat generated are also more. At this time, the temperature is controlled at 22 to 27 degrees Celsius, and the humidity is controlled. Controlled at 50% to facilitate the continued growth of mycelium. The mycelium is grown to a diameter of 8 cm, which is suitable for picking, and can be harvested from the birch mycelium product.

各實施例固態培養基含有不同比例之中藥組合物，白樺茸從植入菌株至菌絲體生長至直徑3公分大小的天數，稱為第一階段，使用一般培養基約需耗時10至20日。而白樺茸菌絲體直徑3公分生長至直徑8公分大小最適採摘階段的天數，稱為第二階段，使用一般培養基約需耗時10至15日。本實驗例是針對各實施例固態培養基所培養之九批白樺茸，統計各批白樺茸分別於第一階段以及第二階段所需的生長天數，並且計算各階段平均天數與進行比較，實驗結果請參照以下表1 The solid medium of each example contains different proportions of the traditional Chinese medicine composition, and the number of days in which Chaga grows from the implanted strain to the mycelium to a diameter of 3 cm is called the first stage, and it takes about 10 to 20 days to use the general medium. The number of days in which the diameter of the Chaga mycelium grows to 3 cm in diameter and is optimal for the picking stage is called the second stage. It takes about 10 to 15 days to use the general medium. This experimental example is for the nine batches of Chaga cultivated in the solid medium of each example, and the number of days of growth of each batch of Chaga in the first stage and the second stage are counted, and the average number of days in each stage is calculated and compared. Please refer to Table 1 below

使用一般培養基培養白樺茸菌絲體，其第一階段以及第二階段所需的總生長天數通常超過35日。然而根據上述實驗例，可知所有實施例之平均培養天數均小於35日，其中實施例5之配方係以重量百分濃度為43%中草藥組合物取代部分樺樹皮，證明為最適合用於培養白樺茸之配方，在提高白樺茸菌絲體生長速度並縮短採收時間的方面具有顯著的效果。 The cultivation of Chaga mycelium using a general medium usually requires a total growth period of more than 35 days in the first stage and the second stage. However, according to the above experimental examples, it can be seen that the average culture days of all the examples are less than 35 days, wherein the formulation of Example 5 replaces part of the birch bark with 43% by weight of the Chinese herbal medicine composition, which proves to be the most suitable for cultivating Chaga. The formula has a remarkable effect in improving the growth rate of the beetle mycelium and shortening the harvesting time.

綜上所述，本發明之白樺茸固態培養基及其製備方法，具有縮短白樺茸採收時間之顯著功效。 In summary, the Chaga solid medium of the present invention and the preparation method thereof have the remarkable effect of shortening the harvesting time of the Chaga.

Claims (7)

一種白樺茸的培養基，其至少包括：一乾料部分，其重量百分比濃度為40%，其中該乾料部分包含：(a)樺樹皮，其重量百分濃度約為30%~40%；(b)綠豆粉，其重量百分濃度為5%~15%；(c)葡萄醣，其重量百分濃度為1%~5%；(d)磷酸二氫鉀，其重量百分濃度為0.05%~1%；(e)硫酸鎂，其重量百分濃度為0.05%~1%；(f)中草藥組合物，其重量百分濃度為20%~50%，其中該中藥組合物係由重量百分濃度25~40%的黃耆、20~35%的紫草、10%~30%的生薑、10%~30%的山藥以及5%~15%的玄明粉所組成；以及一含膠體水溶液，其重量百分比濃度為60%，並且含有體積百分濃度為1%~5%的瓊脂。 A Chaga culture medium comprising at least: a dry material portion having a concentration by weight of 40%, wherein the dry material portion comprises: (a) birch bark, the weight percentage of which is about 30% to 40%; (b Mung bean powder, the weight percentage concentration is 5%~15%; (c) glucose, the weight percentage concentration is 1%~5%; (d) potassium dihydrogen phosphate, the weight percentage concentration is 0.05%~ 1%; (e) magnesium sulfate, the concentration by weight is 0.05% to 1%; (f) the Chinese herbal medicine composition, the concentration by weight is 20% to 50%, wherein the traditional Chinese medicine composition is from weight percent 25~40% of Astragalus, 20~35% of comfrey, 10%~30% of ginger, 10%~30% of yam and 5%~15% of Xuanming powder; and a colloidal aqueous solution It has a concentration of 60% by weight and contains agar with a concentration of 1% to 5% by volume. 如申請專利範圍第1項所述之培養基，其中該樺樹皮呈粉末狀，係由樺木屬植物之樹皮經乾燥與研磨所製備者。 The medium according to claim 1, wherein the birch bark is in the form of a powder, which is prepared by drying and grinding the bark of a birch plant. 如申請專利範圍第1項所述之培養基，其中該培養基之酸鹼值介於約6至7。 The medium of claim 1, wherein the medium has a pH of between about 6 and 7. 如申請專利範圍第1項所述之培養基，其中該樺樹皮之重量百分濃度約為40%；該綠豆粉之重量百分濃度為12.25%；該葡萄醣之重量百分濃度為2.75%；該磷酸二氫鉀之重量百分濃度為1%；該硫酸鎂之重量百分濃度為1%；該中草藥組合物之重量百分濃度為43%；以及該含膠體水溶液具有體積百分濃度為4.6%的瓊脂。 The medium of claim 1, wherein the birch bark has a weight percent concentration of about 40%; the mung bean powder has a weight percent concentration of 12.25%; and the glucose has a weight percent concentration of 2.75%; The weight percent concentration of potassium dihydrogen phosphate is 1%; the weight percent concentration of the magnesium sulfate is 1%; the weight percent concentration of the herbal composition is 43%; and the aqueous colloidal solution has a volume percent concentration of 4.6. % agar. 如申請專利範圍第1項所述之培養基，其中該中草藥組合物係由重量百分濃度30%的黃耆、30%的紫草、15%的生薑、15%的山藥以及10%的玄明粉所組成。 The medium according to claim 1, wherein the Chinese herbal medicine composition is 30% by weight of scutellaria, 30% comfrey, 15% ginger, 15% yam, and 10% Xuanming. Made up of powder. 一種白樺茸之培養基的製備方法，其步驟包括：(A)製備乾料部分，其係依下列各組份預先混合：其重量百分濃度約為30%~40%之乾燥樺樹皮；重量百分濃度為5%~15%之綠豆粉；重量百分濃度為1%~5%之葡萄醣；重量百分濃度為0.05%~1%之磷酸二氫鉀；重量百分濃度為0.05%~1%之硫酸鎂；以及重量百分濃度為20%~50%之中草藥組合物，其中該中藥組合物係由重量百分濃度25~40%的黃耆、20~35%的紫草、10%~30%的生薑、10%~30%的山藥以及5%~15%的玄明粉所組成；(B)將乾料部分滅菌處理；(C)製備含膠體水溶液，其中該含膠體水溶液含有體積百分濃度為1%~5%的瓊脂，並且該水溶液之總重量為該乾料部分1.5倍，並且(D)混合含膠體水溶液以及乾料部分以形成一初始培養基；以及(E)將該初始培養基經滅菌處理。 A method for preparing a culture medium of Chaga, comprising the steps of: (A) preparing a dry material portion which is pre-mixed according to the following components: dry birch bark having a concentration by weight of about 30% to 40%; Mung bean powder with a concentration of 5%~15%; glucose concentration of 1%~5% by weight; potassium dihydrogen phosphate of 0.05%~1% by weight; concentration by weight 0.05%~1 % of magnesium sulfate; and a concentration of 20% to 50% by weight of the herbal composition, wherein the traditional Chinese medicine composition is from 25 to 40% by weight of jaundice, 20 to 35% of comfrey, 10% ~30% ginger, 10%~30% yam and 5%~15% Xuanming powder; (B) partially sterilized dry material; (C) preparation of colloidal aqueous solution, wherein the colloidal aqueous solution contains a volume percent concentration of 1% to 5% agar, and the total weight of the aqueous solution is 1.5 times the dry portion, and (D) mixing the colloidal aqueous solution and the dry portion to form an initial medium; and (E) The initial medium is sterilized. 如申請專利範圍第6項所述之製備方法，其中該培養基之酸鹼值介於約6至7。 The preparation method of claim 6, wherein the medium has a pH of between about 6 and 7.
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