CN115607495A

CN115607495A - Whitening acne-removing mask and preparation method thereof

Info

Publication number: CN115607495A
Application number: CN202211422125.4A
Authority: CN
Inventors: 贺松林
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-11-15
Filing date: 2022-11-15
Publication date: 2023-01-17

Abstract

The invention discloses a whitening acne-removing mask and a preparation method thereof. The whitening acne-removing mask prepared by the invention comprises the following raw materials in parts by weight: 2 to 5 parts of chitosan, 2 to 3 parts of wheat germ powder, 5 to 7 parts of sodium alginate, 2 to 4 parts of xanthan gum, 40 to 50 parts of scutellaria baicalensis fermentation water extract, 0.3 to 0.5 part of cumin-blackland licorice compound, 0.1 to 0.15 part of disodium ethylene diamine tetraacetate, 0.05 to 0.1 part of hyaluronic acid, 0.1 to 0.2 part of sodium carboxymethylcellulose, 0.1 to 0.2 part of salicylic acid, 0.1 to 0.2 part of vitamin C, 0.1 to 0.2 part of water-soluble azone, 0.2 to 0.3 part of preservative, 8 to 10 parts of polyhydric alcohol and 20 to 30 parts of water. Compared with the prior art, the whitening and acne-removing mask prepared by the invention is safe in components, mild, non-irritant and good in stability, and can achieve a good acne-removing effect while whitening skin.

Description

Whitening acne-removing mask and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to a whitening and acne removing mask and a preparation method thereof.

Background

The facial mask is a skin care product which is popular with beauty lovers due to the advantages of convenient carrying, obvious effect and the like. The action mechanism of the mask comprises the following three aspects: firstly, the facial mask keeps full nutrition and moisture of facial skin and enhances the elasticity and vitality of the skin by blocking the contact of the skin and air and inhibiting the evaporation of sweat; secondly, a large amount of water in the mask can fully moisten the skin cuticle, so that the permeability of the cuticle is enhanced, nutrient substances in the mask can effectively permeate into the skin, and the metabolism of epithelial tissue cells is promoted; in addition, the facial mask has an adhesion function, when the facial mask is removed, skin dirt (epidermal cell metabolites, excessive sebum, residual makeup and the like) is adhered and removed together with the facial mask, so that hair follicles of the skin are smooth, and the sebum is smoothly discharged. Therefore, the facial mask can effectively improve the water deficiency and dullness of the skin, reduce the generation of fine wrinkles, delay the skin aging and achieve the effects of removing spots and acnes to a certain extent by scientifically and reasonably using the facial mask.

The facial mask has various classification methods, and can be classified into a moisturizing facial mask, a whitening and freckle-removing facial mask, an anti-wrinkle facial mask, an acne-removing facial mask, a cleansing facial mask, a pore-tightening facial mask and the like according to the efficacy of the facial mask; the facial mask can be classified into peel-off facial mask, paste facial mask, powder facial mask and wet cloth facial mask according to the dosage form of the facial mask; according to the material of the mask carrier, the mask can be divided into a paste type wet cloth-shaped mask and a non-paste type mask; it can be divided into soft film and hard film according to its physical and chemical properties.

Whelk is a chronic inflammatory reaction of a pilosebaceous unit caused by single or mutual action of various factors, and is better developed on the face, the chest, the back and other parts with rich sebaceous glands. At present, the acne-removing mask mainly plays a role through the following five types of functional components: the first category is cutin treating agent, such as salicylic acid (safe use concentration is 0.5% -2%), alpha-hydroxy acid (glycolic acid, lactic acid, citric acid), white willow extract, azelaic acid, papain, etc., to remove superfluous cutin cell of epidermis and dredge pore. The second category is sebum secretion inhibitor, such as zinc salt (zinc sulfate, zinc gluconate, zinc glycyrrhetate, zinc pyrithione), sulfur, vitamin B6, vitamin B3, nicotinamide, soybean isoflavone, tanshinone extract, which can reduce or inhibit excessive secretion of sebum, prevent hair follicle obstruction, and prevent mass propagation of Propionibacterium acnes. The third category is a bactericide, and commonly used bactericidal components comprise zinc salts (zinc sulfate, zinc gluconate, zinc glycyrrhetate and zinc pyrithione), sulfur, azelaic acid, sweet wormwood volatile oil, clove essential oil, rosemary essential oil, tea tree essential oil, propolis, capsaicin and antibacterial peptide, can quickly kill skin parasitic flora such as propionibacterium acnes, mites, pityrosporum orbiculare, staphylococcus aureus and the like, and prevent inflammation and acne growth caused by bacterial pathogen erosion. The fourth category is antiphlogistic, and the common components comprise tea tree extract, delphinium extract, witch hazel extract and the like, and can inhibit inflammatory factors in hair follicles and avoid papules, pustules and serious whelks of nodules. The fifth category is whitening agents to remove residual scars from whelk, and the commonly used whitening agents include arbutin, azelaic acid and derivatives thereof, glucosamine, licorice extract, linoleic acid, vitamin C derivatives, vitamin E derivatives, green tea extract, and the like.

Patent CN 108078897A discloses a houttuynia cordata acne-removing mask which is prepared from the following materials in parts by weight: 30-50 parts of houttuynia cordata extract, 5-10 parts of seaweed extract, 5-10 parts of honeysuckle extract, 2-5 parts of chamomile, 2-5 parts of gastrodia elata, 5-15 parts of aloe gel, 1-5 parts of vegetable protein powder and 15-20 parts of honey. The houttuynia cordata acne-removing mask provided by the invention is purely a natural mask, has no irritation, no dependence and rich raw materials, and has the effects of effectively removing acne, repairing fine lines and reconstructing skin textures; patent CN 103637968A discloses a traditional Chinese medicine acne-removing mask, which comprises the following components by weight: 20-30 parts of radix angelicae powder, 20-30 parts of honey, 20-30 parts of rhubarb powder, 10-20 parts of dandelion powder, 5-30 parts of aloe juice, 3-5 parts of green tea powder and 10-15 parts of deionized water. At present, most acne-removing facial masks mainly comprise traditional Chinese medicine components and pursue pure natural components, so that the problems of instability and poor moisturizing effect are easy to occur. Therefore, the mask which is mild in components, free of stimulation, stable in property and good in acne removing and moisturizing effects has a very wide market prospect.

Disclosure of Invention

In view of the above defects of the prior art, the invention aims to provide a whitening acne-removing mask and a preparation method thereof.

The technical scheme of the invention is as follows:

a whitening and acne removing mask is composed of the following raw materials: chitosan, wheat germ powder, sodium alginate, xanthan gum, scutellaria baicalensis fermentation water extract, ethylene diamine tetraacetic acid, hyaluronic acid, sodium carboxymethylcellulose, salicylic acid, vitamin C, water-soluble azone, a preservative, polyhydric alcohol and water.

Preferably, the whitening acne-removing mask comprises the following raw materials in parts by weight: 2 to 5 parts of chitosan, 2 to 3 parts of wheat germ powder, 5 to 7 parts of sodium alginate, 2 to 4 parts of xanthan gum, 40 to 50 parts of scutellaria baicalensis fermentation water extract, 0.1 to 0.15 part of disodium ethylene diamine tetraacetate, 0.05 to 0.1 part of hyaluronic acid, 0.1 to 0.2 part of sodium carboxymethylcellulose, 0.1 to 0.2 part of salicylic acid, 0.1 to 0.2 part of vitamin C, 0.1 to 0.2 part of water-soluble azone, 0.2 to 0.3 part of preservative, 8 to 10 parts of polyhydric alcohol and 20 to 30 parts of water.

Cumin is rich in various bioactive components, and related researches prove that more than 100 compounds exist in cumin. Research shows that the dried cumin seeds contain volatile oil (5%), fat (22%), protein (10%), saccharides, fiber (11%), free amino acids and various trace elements. Cuminum cyminum seed is rich in flavone and polyphenol substances, including gallic acid, cinnamic acid, rosmarinic acid, coumaric acid and vanillic acid, luteolin, catechin, coumarin, quercetin and quercetin, etc. The cumin non-essential oil mainly comprises terpenes, sesquiterpenes, aromatic aldehydes and ketone ethers. Mainly comprises a plurality of chemical substances such as cuminaldehyde, cuminol, p-cymene, beta-pinene, alpha-pinene, gamma-terpinene, phellandrene, myrcene, terpinene, safranal, limonene and the like, wherein the content of the cuminaldehyde is the highest. The flavonoids and polyphenols have strong oxidation resistance due to the abundant active functional groups such as hydroxyl, phenolic hydroxyl and the like, and can achieve good oxidation resistance through direct removal of oxygen free radicals or excited oxygen, inhibition of the activity of oxidase and chelation of metal ions.

Glycyrrhiza uralensis Fisch, glycyrrhrizae radix mainly contains three mushrooms, flavones and polysaccharides, and also contains more than 100 effective chemical components such as coumarins, amino acids, alkaloids, sex hormones and organic acids. The three mushroom compounds mainly contain glycyrrhizin, glycyrrhetinic acid, alcohol of glycyrrhiza uralensis, isoliquiritin, oleanolic acid, etc. The flavonoids mainly contain liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin (isoliquiritigenin), liquiritigenin (liquiritigenin), neoisoliquiritin, isoformononetin, neoliquiritigenin, licoflavone A and licochalcone A and B. The alkaloid compounds include tetrahydroquinoline compounds: 5,6,7,8-tetrahydro-2,4-dimethylquinoline, 5,6,7,3-tetrahydro-4-methylquinoline. The isoliquiritigenin is used as the highest active ingredient in licoflavone, and has extremely strong tyrosinase inhibitory activity due to abundant phenolic hydroxyl groups contained in the structure of the isoliquiritigenin.

Further, the whitening acne-removing mask comprises the following raw materials in parts by weight: 2 to 5 parts of chitosan, 2 to 3 parts of wheat germ powder, 5 to 7 parts of sodium alginate, 2 to 4 parts of xanthan gum, 40 to 50 parts of scutellaria baicalensis fermentation water extract, 0.3 to 0.5 part of cumin extract, 0.4 to 0.6 part of Ural licorice extract, 0.1 to 0.15 part of disodium ethylene diamine tetraacetate, 0.05 to 0.1 part of hyaluronic acid, 0.1 to 0.2 part of sodium carboxymethylcellulose, 0.1 to 0.2 part of salicylic acid, 0.1 to 0.2 part of vitamin C, 0.1 to 0.2 part of water-soluble azone, 0.1 to 0.2 part of preservative, 8 to 10 parts of polyhydric alcohol and 20 to 30 parts of water.

Preferably, the whitening and acne-removing mask comprises the following raw materials in parts by weight: 2 to 5 parts of chitosan, 2 to 3 parts of wheat germ powder, 5 to 7 parts of sodium alginate, 2 to 4 parts of xanthan gum, 40 to 50 parts of scutellaria baicalensis fermentation water extract, 0.4 to 0.6 part of cumin-Ural licorice compound, 0.1 to 0.15 part of disodium ethylene diamine tetraacetate, 0.05 to 0.1 part of hyaluronic acid, 0.1 to 0.2 part of sodium carboxymethylcellulose, 0.1 to 0.2 part of vitamin C, 0.1 to 0.2 part of water-soluble azone, 0.2 to 0.3 part of preservative, 8 to 10 parts of polyhydric alcohol and 60 to 90 parts of water.

The cumin extract is prepared by the following method: weighing 200-400g of cumin seeds, crushing, sieving with a 50-60-mesh sieve, and mixing the materials according to a material-liquid ratio of 1: (10 to 15) g/mL of the cumin extract is added with an ethanol water solution with the volume fraction of 60 to 70 percent, the mixture is extracted for 2 to 3 hours at the temperature of 70 to 80 ℃, the extracting solution is centrifuged for 10 to 20min at the rotating speed of 4000 to 5000rpm, and the supernatant is rotated, evaporated and concentrated to be dry at the temperature of 40 to 50 ℃ to obtain the cumin extract.

The glycyrrhiza uralensis extract is prepared by the following method: cleaning 500 to 600g Ural licorice root, crushing, sieving by a 60 to 80-mesh sieve, adding 0.5 to 1mol/L HCl aqueous solution, and mixing the materials according to a feed-liquid ratio of 1: (5 to 10) g/mL, hydrolyzing at 90 to 100 ℃ for 2 to 3 hours, adjusting the pH to neutral with 0.5 to 1mol/L NaOH aqueous solution, filtering, adding 5 to 6L ethanol aqueous solution with volume fraction of 70 to 80% into filter residue, performing ultrasonic extraction for 20 to 30min, performing ultrasonic power of 400 to 500W and frequency of 30 to 40Hz, and performing rotary evaporation concentration on the extracting solution at 45 to 50 ℃ until the extracting solution is dry to obtain the Uralen licorice extract.

The cumin-Ural licorice compound is prepared by the following method: adding 30 to 50g of cumin extract and 40 to 60g of Ural licorice extract into 1 to 2L of ethanol aqueous solution with volume fraction of 70 to 80%, adding 5 to 10g of salicylic acid, stirring at 20 to 30 ℃ for 1 to 2h, extracting with ethyl acetate for 2 to 4 times, combining the extracts, and performing rotary evaporation and concentration at 40 to 50 ℃ until the mixture is dry to obtain the cumin-Ural licorice compound.

Preferably, the polyhydric alcohol is 1, 2-propanediol and/or butanediol; more preferably, the weight ratio of the 1, 2-propylene glycol to the butanediol is (0.75 to 1): 1.

preferably, the preservative is at least one of ethylparaben, cason and phenoxyethanol. More preferably, the weight ratio of the ethylparaben to the kalong is (0.1 to 0.15): 0.1.

the invention also provides a preparation method of the whitening acne-removing mask, which comprises the following steps:

s1, preparation of paste: taking polyhydric alcohol, hyaluronic acid, sodium carboxymethylcellulose, ethylparaben, kasong, disodium ethylene diamine tetraacetate, a Ural licorice-cumin extraction compound, chitosan, wheat germ powder, sodium alginate and xanthan gum according to the formula ratio, adding 20 to 30 parts by weight of water, and stirring at 50 to 60 ℃ for 1 to 2h to obtain a paste;

s2, forming of the facial mask: pouring the viscous paste into a mask mold to prepare a film, and then drying at 50-60 ℃ until the water content is 8-10% to obtain a film;

s3, preparing a membrane sheet: and (3) soaking the membrane obtained in the step (S2) in 40-50 parts by weight of scutellaria baicalensis fermentation water extract for 10-12h, then freeze-drying, sterilizing and packaging to obtain the mask.

The preparation method of the scutellaria baicalensis fermentation water extract comprises the following steps: adding 20 to 25g of scutellaria baicalensis aqueous extract into 1 to 1.2L of sugar-free PDB culture medium, inoculating 2 to 3wt% of penicillium oxalate, controlling the temperature to 28 to 30 ℃, fermenting for 70 to 72h, converting at 36 to 37 ℃ for 20 to 24h, centrifuging at 2800 to 3000rpm for 10 to 15min, taking supernatant, filtering at 0.22 mu m, freeze-drying to obtain scutellaria baicalensis fermentation aqueous extract, adding 5 to 10g of scutellaria baicalensis fermentation aqueous extract into 100 to 120mL of water, and mixing uniformly to obtain scutellaria baicalensis fermentation aqueous extract.

The sugar-free PDB culture medium is prepared by the following method: cleaning 200g fresh potato, peeling, cutting into small granules, heating to boil for 30min, filtering to remove potato residue, and diluting to 1L. The prepared PDB medium was sterilized at 115 ℃ for 30min.

The invention provides a whitening and acne removing mask. Compared with the prior art, the invention solves the difficulties in the prior art and has the following beneficial effects:

(1) The whitening and acne-removing mask prepared by the invention has the advantages of simple formula, low raw material cost, simple preparation method, easily-controlled conditions, stable process, industrial production and popularization and application;

(2) The whitening acne-removing mask disclosed by the invention can well moisten and nourish the skin, has a good whitening acne-removing effect, can keep the moisture of the skin, has a weak pH value, can mildly care the skin, and does not generate an irritant reaction;

(3) The whitening acne-removing mask disclosed by the invention is prepared by mixing the Ural licorice extract and the cumin extract, cuminaldehyde in the cumin extract is combined with isoliquiritigenin generated by acid hydrolysis of licorice, so that the content of flavonoid compounds is increased, a conjugated olefin structure is formed after combination, phenolic hydroxyl groups are not influenced, the structure is stable, the activity is stronger, the effects of inhibiting tyrosinase activity and melanin synthesis are good, the skin can be well whitened, and meanwhile, the scutellaria baicalensis is taken as a classical Chinese herbal medicine, so that the whitening acne-removing mask has the effects of resisting bacteria, resisting allergy, resisting inflammation and resisting tumors. And the conversion of partial functional substances in the scutellaria baicalensis can be further enhanced through fermentation treatment, and the functional substances can be added into the mask disclosed by the invention to play a better whitening and acne removing effect under the combined action of various active ingredients. And the components are mild and have low irritation, so the method has good market prospect and popularization value.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention. The operations referred to in the examples are, unless otherwise specified, all those of ordinary skill in the art.

In the embodiment of the invention, part of the raw material parameters are as follows:

water soluble azone, CAS number: 59227-89-3, which is from the company of limited liability of the pharmaceutical industry of the science of the Xian Ming Dynasty.

Cason, CAS No.: 26172-55-4, from Qifei pharmaceutical chemical Limited in lake north.

Baical skullcap root aqueous extract was purchased from Shanxi YuLai Biotech Ltd.

Wheat germ flour, 100 mesh, was purchased from Biotechnology Ltd, a source of Hebei.

Penicillium oxalicum, penicillium oxyalicum, CICC 41676, from the China center for the Collection of Industrial microorganisms

Comparative example 1

The preparation method of the whitening and acne-removing mask comprises the following steps:

s1, preparation of paste: taking 3g of 1, 2-propylene glycol, 0.1g of hyaluronic acid, 0.1g of sodium carboxymethylcellulose, 0.15g of ethylparaben, 0.1g of cason, 0.1g of disodium ethylenediamine tetraacetic acid, 2g of chitosan, 3g of wheat germ powder, 6g of sodium alginate and 2g of xanthan gum, adding 20mL of water, and stirring at 60 ℃ for 2 hours to obtain a paste;

s2, forming of the facial mask: pouring the viscous paste into facial mask mold to make into membrane, drying at 60 deg.C until water content is 8% to obtain membrane, freeze drying at-40 deg.C for 6h, sterilizing at 110 deg.C for 15min, cooling, and packaging to obtain facial mask.

Comparative example 2

s1, preparation of paste: taking 3g of 1, 2-propylene glycol, 0.1g of hyaluronic acid, 0.1g of sodium carboxymethylcellulose, 0.15g of ethylparaben, 0.1g of carbazone, 0.1g of disodium ethylenediamine tetraacetic acid, 0.4g of a Ural licorice-cumin extraction compound, 2g of chitosan, 3g of wheat germ powder, 6g of sodium alginate and 2g of xanthan gum, adding 20mL of water, and stirring at 60 ℃ for 2 hours to obtain a paste;

s2, forming the facial mask: pouring the viscous paste into facial mask mold to make into membrane, drying at 60 deg.C until water content is 8% to obtain membrane, freeze drying at-40 deg.C for 6h, sterilizing at 110 deg.C for 15min, cooling, and packaging to obtain facial mask.

The compound of Wular liquorice and cumin is prepared by the following method:

(1) Weighing 200g of cumin seeds, crushing, sieving with a 60-mesh sieve, adding 3L of 70% ethanol aqueous solution by volume, extracting at 70 ℃ for 2h, centrifuging the extracting solution at 4000rpm for 10min, and concentrating the supernatant at 50 ℃ until the supernatant is dry to obtain a cumin extract;

(2) Cleaning 500g of Ural licorice root, crushing, sieving with a 60-mesh sieve, adding 3L of 0.5mol/L HCl solution, hydrolyzing at 90 ℃ for 2h, adjusting pH to be neutral by using 0.5mol/L NaOH aqueous solution, filtering, adding 5L of 80% ethanol aqueous solution in volume fraction into filter residue, performing ultrasonic extraction for 20min, performing ultrasonic power of 400W and frequency of 40Hz, and concentrating the extracting solution at 50 ℃ until the extracting solution is dried to obtain an Ural licorice extract;

(3) Adding 30g of cumin extract and 40g of Ural licorice extract into 1L of 80 volume percent ethanol water solution, adding 5g of salicylic acid, stirring for 2h at 20 ℃, extracting with 500mL of 3 ethyl acetate, combining the extract, and concentrating to dryness at 45 ℃ to obtain the cumin-Ural licorice compound.

Example 1

s1, preparation of paste: taking 3g of 1, 2-propylene glycol, 0.1g of hyaluronic acid, 0.1g of sodium carboxymethylcellulose, 0.15g of ethylparaben, 0.1g of cason, 0.1g of disodium ethylenediamine tetraacetic acid, 0.4g of cumin extract, 2g of chitosan, 3g of wheat germ powder, 6g of sodium alginate and 2g of xanthan gum, adding 20mL of water, and stirring at 60 ℃ for 2h to obtain a paste;

The cumin extract is prepared by the following method: weighing 200g of cumin seeds, crushing, sieving by a 60-mesh sieve, adding 3L of 70% ethanol water solution by volume fraction, extracting at 70 ℃ for 2h, centrifuging the extracting solution at 4000rpm for 10min, and concentrating the supernatant at 50 ℃ until the supernatant is dry to obtain the cumin extract.

Example 2

s1, preparation of paste: taking 3g of 1, 2-propylene glycol, 0.1g of hyaluronic acid, 0.1g of sodium carboxymethylcellulose, 0.15g of ethylparaben, 0.1g of cason, 0.1g of disodium ethylenediaminetetraacetate, 0.4g of a compound extracted from Glycyrrhiza uralensis and Cuminum celery, 2g of chitosan, 3g of wheat germ powder, 6g of sodium alginate and 2g of xanthan gum, adding 20mL of water, and stirring for 2 hours at 60 ℃ to obtain a paste body;

The compound of Wular liquorice and cumin is prepared by the following method:

(1) Weighing 200g of cumin seeds, crushing, sieving with a 60-mesh sieve, adding 3L of 70% ethanol aqueous solution by volume fraction, extracting at 70 ℃ for 2h, centrifuging the extracting solution at 4000rpm for 10min, and concentrating the supernatant at 50 ℃ until the supernatant is dry to obtain a cumin extract;

(2) Cleaning 500g of Ural licorice root, crushing, sieving with a 60-mesh sieve, adding 3L of 0.5mol/L HCl aqueous solution, hydrolyzing at 90 ℃ for 2h, adjusting pH to neutral with 0.5mol/L NaOH aqueous solution, filtering, adding 5L of 80% ethanol aqueous solution with volume fraction into filter residue, performing ultrasonic extraction for 20min at the ultrasonic power of 400W and the frequency of 40Hz, and concentrating the extract at 50 ℃ to dryness to obtain Ural licorice extract;

(3) Adding 30g of cumin extract and 40g of Ural licorice extract into 1L of anhydrous methanol, adding 100mL of 25wt% NaOH aqueous solution, keeping out of the sun, stirring for 50h at 50 ℃, standing for 10min, adding 2mol/L HCl aqueous solution to adjust the pH value to be neutral, adding 500mL of ethyl acetate for extraction, washing the extract with 400mL of 3 water for three times, removing ethyl acetate at 45 ℃ to obtain a crude product, further adding 100mL of toluene for washing, washing with 100mL of 3 water for 3 times to remove toluene, and performing vacuum drying at 60 ℃ to obtain the Ural licorice-cumin extract compound.

Under the condition of weak alkalinity, aldehydes such as cuminaldehyde in the cumin extract can be coupled with isoliquiritigenin which is obtained after the licorice extract is hydrolyzed by acid to form unsaturated conjugated double bonds, the structure of the formed flavonoid compound is more stable, and the holding activity of the conjugated double bonds is higher. Meanwhile, the invention utilizes the aldehydes without antioxidant activity in the cumin extract, enhances the antioxidant activity of the compound, can obviously improve the antioxidant activity of the product when being applied to cosmetics, and obviously improves the whitening effect.

Example 3

s1, preparing paste: taking 3g of 1, 2-propylene glycol, 0.1g of hyaluronic acid, 0.1g of sodium carboxymethylcellulose, 0.15g of ethylparaben, 0.1g of cason, 0.1g of disodium ethylenediaminetetraacetate, 0.4g of glycyrrhiza uralensis extract, 2g of chitosan, 3g of wheat germ powder, 6g of sodium alginate and 2g of xanthan gum, adding 20mL of water, and stirring at 60 ℃ for 2 hours to obtain a paste body;

The glycyrrhiza uralensis extract is prepared by the following method: cleaning 500g of Ural licorice root, crushing, sieving with a 60-mesh sieve, adding 3L of 0.5mol/L HCl aqueous solution, hydrolyzing at 90 ℃ for 2h, adjusting pH to be neutral by using 0.5mol/L NaOH aqueous solution, filtering, adding 5L of ethanol aqueous solution with volume fraction of 80% into filter residue, performing ultrasonic extraction for 20min, performing ultrasonic power of 400W and frequency of 40Hz, and concentrating the extracting solution at 50 ℃ to be dry to obtain the Ural licorice extract.

Example 4

s2, forming of the facial mask: pouring the viscous paste into a mask mold to prepare a mask, and then drying at 60 ℃ until the water content is 8% to obtain a diaphragm;

s3, preparing the facial mask: and (3) soaking the membrane obtained in the step (S2) in the scutellaria baicalensis fermentation water extract for 12h, then freeze-drying at-40 ℃ for 6h, sterilizing at 110 ℃ for 15min, cooling and packaging to obtain the facial mask.

The preparation method of the scutellaria baicalensis fermentation water extract comprises the following steps: adding 20g of scutellaria baicalensis aqueous extract into 1L of sugar-free PDB culture medium, inoculating 2wt% of penicillium oxalate, fermenting for 72h at the temperature of 28 ℃, converting for 24h at 37 ℃, centrifuging for 10min at 3000rpm, taking supernatant, filtering at 0.22 mu m, freeze-drying to obtain scutellaria baicalensis fermented aqueous extract, adding 8g of scutellaria baicalensis fermented aqueous extract into 100mL of water, and uniformly mixing to obtain scutellaria baicalensis fermented aqueous extract.

The sugar-free PDB culture medium is prepared by the following method: cleaning 200g of fresh potatoes, peeling and cutting into small granules, heating and boiling for 30min, filtering to remove potato dregs, and metering to 1L; the prepared PDB medium was sterilized at 115 ℃ for 30min.

The compound of Ural licorice and cumin is prepared by the following method:

(2) Cleaning 500g of Ural licorice root, crushing, sieving with a 60-mesh sieve, adding 3L of 0.5mol/L HCl aqueous solution, hydrolyzing at 90 ℃ for 2h, adjusting pH to be neutral by using 0.5mol/L NaOH aqueous solution, filtering, adding 5L of ethanol aqueous solution with volume fraction of 80% into filter residue, performing ultrasonic extraction for 20min, wherein the ultrasonic power is 400W, the frequency is 40Hz, and concentrating the extracting solution at 50 ℃ to be dry to obtain the Ural licorice extract;

Under the condition of weak alkalinity, aldehydes such as cuminaldehyde in the cumin extract can be coupled with isoliquiritigenin which is obtained after the licorice extract is hydrolyzed by acid to form unsaturated conjugated double bonds, the structure of the formed flavonoid compound is more stable, and the holding activity of the conjugated double bonds is higher. Meanwhile, the aldehydes without antioxidant activity in the cumin extract are utilized, so that the antioxidant activity of the compound is enhanced, the antioxidant activity of the product can be obviously improved when the cumin extract is applied to cosmetics, and the whitening effect can also be obviously improved.

Test example 1

The comparative examples and examples were tested for tyrosinase inhibitory activity according to the test method of the national society for daily chemicals, standard T/SHRH 015-2018. The method comprises the following specific steps: setting a sample tube (T) and a sample background (T) ₀ ) Enzyme reaction tube (C) and solvent background (C) ₀ ) And three parallel pipes are arranged in each group. In the sample tube (T) and sample background (T) ₀ ) 1mL of the same concentration of each of the sample solutions, an enzyme reaction tube (C) and a solvent background (C) ₀ ) Respectively adding 1mL phosphate buffer solution, respectively adding 0.5mL tyrosinase solution into the sample tube and the enzyme reaction tube, respectively, replacing the sample background and the solvent background with 0.5mL phosphate buffer solution, mixing, incubating at 35 deg.C for 10min, sequentially placing in each tubeAdding 2mL of levodopa solution, controlling the reaction time of the cuvette to be 5min, quickly transferring the cuvette into a cuvette, immediately measuring the absorbance of the reaction solution of each sample at 475 nm by using an ultraviolet-visible spectrophotometer, and calculating the inhibition rate.

Inhibition (%) = (1- (T-T) ₀ ）/（C－C ₀ ））*100%

T-light absorption value of the sample tube, namely the light absorption value of the solution after the sample reacts with tyrosinase;

T ₀ -sample background absorbance;

c, light absorption value of tyrosinase and dopa reaction when no sample is added;

C ₀ -background absorbance of solvent.

TABLE 1 inhibition of tyrosinase Activity results

Experimental protocol	Inhibition rate/%)
		Comparative example 1	46.50%
Comparative example 2	74.98%
		Example 1	55.80%
Example 2	86.32%
		Example 3	56.62%
Example 4	86.50%

As can be seen from table 1, both the glycyrrhiza uralensis and the cumin extract can improve the activity of the mask on tyrosinase, but the inhibition effect of the mask on tyrosinase is more obvious after the glycyrrhiza uralensis and the cumin extract are compounded, compared with the comparative example 2, the cumin-glycyrrhiza uralensis extract is compounded under an alkaline condition to generate a C-C coupling reaction, the combination is tighter, the generated compound has a flavonoid structure, and the oxidation resistance of the compound is stronger due to conjugated double bonds and abundant phenolic hydroxyl groups, so that the better inhibition effect on tyrosinase catalytic activity is achieved, tyrosinase is a first rate-limiting enzyme in a melanin biosynthesis process, the better inhibition effect on tyrosinase is achieved, the better synthesis of melanin can be inhibited, the effects of removing freckles and brightening skin color are achieved, and the whitening effect of the mask can be improved by further adding the scutellaria baicalensis fermentation extract on the basis in the example 4.

Test example 2

The comparative examples and examples were conducted to test the activity of inhibiting the synthesis of melanin, which is a biological pigment produced by melanocytes and widely present in human skin, hair, retina, etc., and normal content of melanin can help the skin from being damaged by ultraviolet rays, but melanin is secreted excessively, resulting in hyperpigmentation of the skin, easily causing freckles, chloasma, skin cancer, etc. Therefore, it is important to suppress the production of melanin or to accelerate the decomposition of existing melanin for improving skin color. The test method refers to the group standard of T/ZHCA 012-2020 < test method for melanin inhibition of zebra fish embryo for cosmetic whitening effect >, 'mouse in water' zebra fish, as a model organism with 87% similarity to human gene, the experimental result based on zebra fish embryo is generally applicable to human body, the zebra fish is transparent in whole body at the early development stage, and melanin begins to grow from the retinal epithelium after the embryo grows to 24 h. Pigment cells originate from neural crest cells differentiated from the back ectoderm and then proliferate, migrate and differentiate into pigment blast cells. The test substance intervenes in the melanin formation process of the zebra fish, the melanin formation can be inhibited, and the melanin inhibition efficiency of the test substance can be calculated by comparing with a normal control group. Selecting normal 6hpf zebra fish embryos, putting the normal 6hpf zebra fish embryos into a six-well plate, removing standard dilution water in the six-well plate under the condition of not damaging the embryos, and quickly adding 3mL of a test substance solution into each well. After the incubation is finished, at least 12 zebra fishes are randomly selected from the zebra fishes with normal phenotype and behaviors, fixed by 3# methyl cellulose, observed under a stereoscopic microscope and photographed, and the positions of all the zebra fishes are kept consistent when photographed. The pictures are analyzed by using image analysis software, and quantitative areas are selected from the head edge (except the eye part) to the part tangent to the yolk sac. And (4) calibrating the analysis parameters to OD values so as to calculate the intensity of melanin signals of the zebra fish head, wherein effective data of each group are not less than 10. Recording the melanin signal intensity as S, and calculating the freckle removing effect of the test object, namely the melanin inhibition rate, wherein the formula is as follows:

melanin inhibition% = (S) _{(Normal control group)} -S _{(test article group)} ）/S _{(Normal control group)}

TABLE 2 Melanin inhibition efficacy test results Table

Experimental protocol	Melanin inhibition/%)
		Comparative example 1	50.02%
Comparative example 2	73.48%
		Example 1	60.56%
Example 2	86.30%
		Example 3	67.47%
Example 4	87.78%

Tyrosinase is a key enzyme in the process of melanin synthesis, inhibits the activity of tyrosinase, and can directly inhibit the synthesis of melanin, however, melanin is generated by the secretion of melanocytes, inhibits the proliferation of melanocytes, and can directly reduce the generation of melanin, so the effect of inhibiting the generation of melanin or accelerating the decomposition of the existing melanin is more direct to improve the skin color problem. It can be known from the table that after the cumin extract and the Ural extract are compounded, the activity capabilities of scavenging free radicals and inhibiting tyrosinase are improved due to the increase of the content of flavonoid compounds, the free radicals can up-regulate the expression of tyrosinase mRNA, so that the activity of tyrosinase is enhanced, oxygen radicals are scavenged, the catalytic activity of tyrosinase can be indirectly inhibited, and the generation of melanin is inhibited, and the flavonoid substances formed in the compounds in the examples 2 and 4 have unique structures, so that the chelating capacity of the flavonoid substances and metal ions of the tyrosinase activity center is greatly enhanced, and can compete with substrates to bind tyrosinase, so that the process of tyrosine catalytic oxidation by tyrosinase can be prevented, and the generation of melanin is reduced.

Test example 3

The anti-acne effect test is carried out on the comparative ratio and the embodiment, 120 volunteers with obvious acnes and acnes on the face are selected, the age is 18-25 years old, the volunteers are randomly divided into 6 groups, and each group comprises 20 people, and the anti-acne facial masks prepared by the embodiment and the comparative ratio are respectively used; the face cleaning cream is used once a day, and after the face is cleaned at night, the face is covered for 5 to 10min; after three months, the effect after use was counted and the results are shown in table 3.

Table 3-acne removing effect test of facial mask table

Experimental protocol	Effective rate%
		Comparative example 1	30.98%
Comparative example 2	35.60%
		Example 1	50.45%
Example 2	75.34%
		Example 3	72.98%
Example 4	90.20%

The method has the following advantages: the face acne and pox are reduced, and no or few new acne and pox grow out; the color of the acne mark is obviously lightened.

And (4) invalidation: the acne and the pox are not obviously changed, occasionally, new acne and pox grow out, and the color of the pox mark is not changed or deepened.

As can be seen from table 3, the whitening mask added with the scutellaria baicalensis fermentation water extract has a good acne removing effect, and scutellaria baicalensis serving as a classic Chinese herbal medicine has the effects of resisting bacteria, allergy, inflammation and tumors. Active substances in the scutellaria baicalensis are mainly flavonoid compounds such as baicalin, baicalein, wogonoside and wogonin, fermentation treatment can enhance conversion of partial functional substances in the scutellaria baicalensis, the scutellaria baicalensis is fermented by a specific flora and then added into a mask, and the mask has excellent acne removing and whitening effects under the combined action of the mask and other components, and the scutellaria baicalensis fermentation product is natural and non-irritant and is suitable for long-term use.

Claims

1. The whitening and acne removing mask is characterized by comprising the following raw materials: chitosan, wheat germ powder, sodium alginate, xanthan gum, scutellaria baicalensis fermentation water extract, a cumin-urale licorice compound, disodium ethylene diamine tetraacetate, hyaluronic acid, sodium carboxymethylcellulose, salicylic acid, vitamin C, water-soluble azone, a preservative, polyhydric alcohol and water.

2. The whitening and acne-removing mask as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 2 to 5 parts of chitosan, 2 to 3 parts of wheat germ powder, 5 to 7 parts of sodium alginate, 2 to 4 parts of xanthan gum, 40 to 50 parts of scutellaria baicalensis fermentation water extract, 0.3 to 0.5 part of cumin-blackland licorice compound, 0.1 to 0.15 part of disodium ethylene diamine tetraacetate, 0.05 to 0.1 part of hyaluronic acid, 0.1 to 0.2 part of sodium carboxymethylcellulose, 0.1 to 0.2 part of salicylic acid, 0.1 to 0.2 part of vitamin C, 0.1 to 0.2 part of water-soluble azone, 0.2 to 0.3 part of preservative, 8 to 10 parts of polyhydric alcohol and 20 to 30 parts of water.

3. The whitening and acne-removing mask as claimed in claim 1 or 2, characterized in that the cumin-Ural licorice compound is prepared by the following method: adding a cumin extract and a Ural licorice extract into an ethanol water solution with the volume fraction of 70-80%, adding salicylic acid, stirring for 1-2h at 20-30 ℃, extracting for 2-4 times by ethyl acetate, combining the extracts, and concentrating to dryness to obtain the cumin-Ural licorice compound.

4. The whitening and acne-removing mask as claimed in claim 3, wherein the cumin extract is prepared by the following method: weighing cumin seeds, crushing, sieving, adding an ethanol water solution with volume fraction of 60-70%, extracting at 70-80 ℃ for 2-3h, centrifuging the extracting solution at 4000-5000 rpm for 10-20min, concentrating the supernatant to be dry, and drying at 60-70 ℃ to obtain the cumin extract.

5. The whitening and acne-removing mask as claimed in claim 3, characterized in that the Glycyrrhiza uralensis Fisch extract is prepared by the following method: cleaning Ural licorice root, crushing, sieving, adding 0.5-1mol/L HCl aqueous solution for hydrolysis for 2-3h, adjusting pH to neutral by 0.5-1mol/L NaOH aqueous solution, filtering, adding filter residue into 70-80% ethanol aqueous solution by volume fraction, carrying out ultrasonic extraction, and concentrating the extract to obtain Ural licorice extract.

6. The mask for whitening and removing acnes according to claim 1 or 2, wherein the scutellaria baicalensis fermentation water extract is prepared by the following method: adding 20-25g of the scutellaria baicalensis aqueous extract into 1-1.2L of sugar-free PDB culture medium, inoculating 2-3wt% of zymocyte, fermenting at 28-30 ℃ for 70-72h, converting at 36-37 ℃ for 20-24h, centrifuging at 2800-3000rpm for 10-15min, taking supernatant, filtering at 0.22 mu m, freeze-drying to obtain a scutellaria baicalensis fermentation aqueous extract, adding 5-10g of the scutellaria baicalensis fermentation aqueous extract into 100-120mL of water, and mixing uniformly to obtain the scutellaria baicalensis fermentation aqueous extract.

7. The whitening and acne-removing mask according to claim 6, wherein the sugar-free PDB culture medium is prepared by the following method: cleaning 200g of fresh potatoes, peeling and cutting into small granules, heating and boiling for 30min, filtering to remove potato dregs, and metering to 1L; the prepared PDB medium was sterilized at 115 ℃ for 30min.

8. The whitening and acne-removing mask according to claim 6, characterized in that: the zymocyte is penicillium oxalicum.

9. The preparation method of the whitening and acne-removing mask as claimed in any one of claims 1 to 8, which is characterized by comprising the following steps:

s1, preparation of paste: taking 20 to 30 parts by weight of water into a formula amount of polyhydric alcohol, hyaluronic acid, sodium carboxymethylcellulose, ethylparaben, kason, disodium ethylene diamine tetraacetate, an extracted compound of Ural licorice and cumin, chitosan, wheat germ powder, sodium alginate and xanthan gum, and stirring at 50 to 60 ℃ for 1 to 2h to obtain a paste;

s2, forming of the facial mask: pouring the viscous paste into a mask mold to prepare a film, and then drying the film at 50-60 ℃ until the water content is 8-10% to obtain a film;

s3, preparing the facial mask: and (3) soaking the membrane obtained in the step (S2) in 40-50 parts by weight of scutellaria baicalensis fermentation water extract for 10-12h, and then carrying out freeze drying, sterilization and packaging to obtain the mask.