CN108245479A - A kind of facial mask containing bifidobacterium lactis fermentation activity extract - Google Patents
A kind of facial mask containing bifidobacterium lactis fermentation activity extract Download PDFInfo
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- CN108245479A CN108245479A CN201710852338.3A CN201710852338A CN108245479A CN 108245479 A CN108245479 A CN 108245479A CN 201710852338 A CN201710852338 A CN 201710852338A CN 108245479 A CN108245479 A CN 108245479A
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- fermentation
- bifidobacterium lactis
- facial mask
- glucose
- peptone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The invention discloses a kind of facial masks containing bifidobacterium lactis fermentation activity extract, the present invention passes through creatively optimization for fermentation technology, and with reference to scientific matching, obtaining antiallergic has the breakthrough effect for surmounting hydrocortisone, additionally has both the facial mask of the regenerated effect of stimulation collagen.The present inventor attempts to add in Conyza japonica saponin(e R in fermentation stage, with reference to the process conditions (such as several kinds of carbon source cooperation) of other optimizations, it was found that the physiological property of bifidobacterium lactis can be changed, the biological polyoses isoreactivity substance that the antiallergic with extremely strong activity promotes regenerated bioactivity is generated.
Description
Technical field
The present invention relates to a kind of facial masks containing bifidobacterium lactis fermentation activity extract, belong to cosmetic field.
Background technology
The influence of global industry and urbanization to environmental protection at present is very big, people of various countries' common concern pollution problem.Performance
It is exactly that people are found with high-tech content, beneficial to skin health again not by the biology of Pollution by Chemicals on cosmetics and skincare product
Technology skin care item.In addition, with the improvement of people's safety consciousness, to avoid the harmful effect to skin, people are more likely to select
Select safe skin nursing products.
All multi-brands have used two and split yeast tunning lysis object in the prior art, have data to show yeast fermentation filtering
Object can mitigate the oxidative damage of skin really under action of ultraviolet radiation, promote skin reparation.The raw material is by passing through biotechnology
The Bifidobacterium lysate composition of synthesis.Metabolite is included from data disclosed in producer, cytoplasm, cell wall and polysaccharide are answered
Object is closed, this active material obtained by biotechnology can specifically support the protection of skin itself and repair matrix.Energy
Enough skin is enabled to provide prevention nursing for normal skin and road, it is ultraviolet to protect into repair system by enhancing endogenous cellular enzymatic
Damage, prevents skin aging from occurring in advance caused by line.
The prior art be also disclosed it is a kind of can effectively facilitate skin renewal, reduce B16 cell, have white-skinned face function, energy
It effectively removes free radical, improve dermal tissue physiological function, there is anti-ageing and higher safety lactobacillus-fermented lysis
Product, preparation method and applications.
Bifidobacterium lactis is a kind of obligate anaerobe, stringent to environmental condition and nutritional requirement, influences its survival and growth
Factor it is also very much.The present invention is intended to provide a kind of facial mask containing bifidobacterium lactis fermentation activity extract, the present invention pass through
Creatively optimization for fermentation technology, and with reference to scientific matching, acquisition antiallergic has the breakthrough effect for surmounting hydrocortisone,
Additionally have both the facial mask of the regenerated effect of stimulation collagen.
Invention content
Technical problems based on background technology, the present invention for background technology there are the problem of, one kind is provided and is contained
The facial mask of bifidobacterium lactis fermentation activity extract, the present invention combine scientific matching by creatively optimization for fermentation technology,
Obtaining antiallergic has the breakthrough effect for surmounting hydrocortisone, additionally has both the face of the regenerated effect of stimulation collagen
Film.
The herb of Conyza japonica system composite family Conyza plant Conyza japonica (Thunb.) Less, face generation human relations etc. were once
Through reporting the isolated compound Conyza japonica saponin(e R from Conyza japonica, Structural Identification is 3-O- β-D-glucopyranosyl
medicagenic acid 28-O-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-[β-D-
Apiofuranosyl- (1 → 3)]-α-L-rhamnopyranosyl- (1 → 2)-α-L-arabinopyranosyl ester,
Its structural formula is：
The synthesis of exocellular polysaccharide can be divided into 2 classes, the synthesis of homologous polysaccharide and the synthesis of heterologous polysaccharides in lactic acid bacteria.It grinds
Study carefully personnel to point out, the high bacterial strain of yield of extracellular polysaccharide, the growth and breeding speed of bacterial strain is generally slower, and produces fragrant ability and souring ability
Also it is general weaker.Homologous polysaccharide is in extracellular synthesis such as glucan, levulan.Heterologous polysaccharides are synthesized on cell membrane
's.
The generation of Exopolysaccharides Produced by Lactic Acid Bacteria is an extremely complex process, utilizes different substrates and fermentation condition, lactic acid
The exocellular polysaccharide of bacterium generation has larger difference.Synthesis of different carbon source, nitrogen source and the inorganic salts to the exocellular polysaccharide of lactic acid bacteria
All have an impact, requirement of the different lactic acid bacterias to these substances is also different.It is believed that nitrogen source in suitable control culture medium
Content is conducive to the synthesis of Exopolysaccharides Produced by Lactic Acid Bacteria.The present inventor attempts to add in Conyza japonica saponin(e R in fermentation stage, with reference to it
The process conditions (such as several kinds of carbon source cooperation) that he optimizes find that the physiological property of bifidobacterium lactis can be changed, generate with pole
Strongly active antiallergic promotes the biological polyoses isoreactivity substance of regenerated bioactivity.
The purpose of the present invention is achieved through the following technical solutions：
A kind of facial mask containing bifidobacterium lactis fermentation activity extract, is prepared by the following method：
(1) bifidobacterium lactis tunning lysis object is prepared：
A, bifidobacterium lactis is spread cultivation in seed culture medium；
The seed culture based formulas：Glucose 30g, peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride
5g, Tween-80 1ml, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, Conyza japonica saponin(e R 0.5g,
1000ml is added water to, adjusts pH to 6.5, sterilizing is spare；
B, the bifidobacterium lactis seed to spread cultivation is seeded in fermentation medium and fermented, obtain zymotic fluid；
Fermentation medium：Glucose 1.8%, trehalose 1%, mannose 0.5%, peptone 1%, acerola concentrate powder
0.5%th, yeast extract 1.5%, Tween-80 0.1%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate
0.019%th, L-cysteine hydrochloride 0.1%, Conyza japonica saponin(e R 0.2%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0, ferment pH:5.0, speed of agitator：80r/min；
C, centrifugal treating：
By zymotic fluid centrifugal treating, centrifugal rotational speed 7000r/min, time 20min topple over supernatant collection bacterium mud；
D, it emulsifies：
According to bacterium mud quality, according to 1:5 addition Trehalosc protection agent, it is fully emulsified through high speed agitator progress, obtain breast
Change liquid；
E, it sterilizes：
Emulsion is subjected to pasteurization, obtains lactobacillus-fermented lysate；
(2) prepare other pure plant components：
Prepare other plant extracting solution and additive, make facial mask liquid.
Preferably, the other plant extracting solution and additive are：Water, sorb (sugar) alcohol, glycerine, glycine betaine, fourth
Glycol, giant knotweed (POLYGONUM CUSPIDATUM) extract, centella (CENTELLA ASIATICA) extract, radix scutellariae
(SCUTELLARIA BAICALENSIS) root extract, tea (CAMELLIA SINENSIS) leaf extract, glycyrrhiza glabra
(GLYCYRRHIZA GLABRA) root extract, german chamomile (CHAMOMILLA RECUTITA) flower extract, rosemary
(ROSMARINUS OFFICINALIS) extract, propylene glycol, xanthans, carbomer, triethanolamine, hydroxyethyl cellulose,
Cremophor RH40, Phenoxyethanol, methyl hydroxybenzoate, ethyl hydroxy benzoate, Sensiva SC50.
The invention has the beneficial effects that：
1) the present inventor attempts to add in Conyza japonica saponin(e R in fermentation stage, and the process conditions with reference to other optimizations are (such as a variety of
Carbon source coordinates), it finds that the physiological property of bifidobacterium lactis can be changed, generates the antiallergic with extremely strong activity and promote regenerated life
The biological polyoses isoreactivity substance of object activity.
2) lactobacillus-fermented lysate of the invention has the antiallergic effect also stronger than hydrocortisone, is to lactic acid bacteria
The important breakthrough utilized.
3) lactobacillus-fermented lysate of the invention has the stronger stimulation regenerated effect of skin collagen.
Description of the drawings：
Fig. 1 is cellular control unit；
There is the performance of degranulation for the post-stimulatory cells of C48/80 in Fig. 2；
Fig. 3 gives cellular morphology after the processing of 25g/L lactobacillus-fermenteds lysate while stimulation for C48/80.
Specific embodiment
Embodiment 1：
Prepare bifidobacterium lactis tunning lysis object：
A, bifidobacterium lactis is spread cultivation in seed culture medium；
The seed culture based formulas：Glucose 30g, peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride
5g, Tween-80 1ml, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, Conyza japonica saponin(e R 0.5g,
1000ml is added water to, adjusts pH to 6.5, sterilizing is spare；
B, the bifidobacterium lactis seed to spread cultivation is seeded in fermentation medium and fermented, obtain zymotic fluid；
Fermentation medium：Glucose 1.8%, trehalose 1%, mannose 0.5%, peptone 1%, acerola concentrate powder
0.5%th, yeast extract 1.5%, Tween-80 0.1%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate
0.019%th, L-cysteine hydrochloride 0.1%, Conyza japonica saponin(e R 0.2%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0, ferment pH:5.0, speed of agitator：80r/min；
C, centrifugal treating：
By zymotic fluid centrifugal treating, centrifugal rotational speed 7000r/min, time 20min topple over supernatant collection bacterium mud；
D, it emulsifies：
According to bacterium mud quality, according to 1:5 addition Trehalosc protection agent, it is fully emulsified through high speed agitator progress, obtain breast
Change liquid；
E, it sterilizes：
Emulsion is subjected to pasteurization, obtains lactobacillus-fermented lysate.
Embodiment 2：
Control group 1, without using Conyza japonica saponin(e R：
Prepare bifidobacterium lactis tunning lysis object：
A, bifidobacterium lactis is spread cultivation in seed culture medium；
The seed culture based formulas：Glucose 30g, peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride
5g, Tween-80 1ml, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, 1000ml is added water to, adjusts pH
To 6.5, sterilizing is spare；
B, the bifidobacterium lactis seed to spread cultivation is seeded in fermentation medium and fermented, obtain zymotic fluid；
Fermentation medium：Glucose 1.8%, trehalose 1%, mannose 0.5%, peptone 1%, acerola concentrate powder
0.5%th, yeast extract 1.5%, Tween-80 0.1%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate
0.019%th, L-cysteine hydrochloride 0.1%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0 ferment pH:5.0, speed of agitator：80r/min；
C, centrifugal treating：
By zymotic fluid centrifugal treating, centrifugal rotational speed 7000r/min, time 20min topple over supernatant collection bacterium mud；
D, it emulsifies：
According to bacterium mud quality, according to 1:5 addition Trehalosc protection agent, it is fully emulsified through high speed agitator progress, obtain breast
Change liquid；
E, it sterilizes：
Emulsion is subjected to pasteurization, obtains lactobacillus-fermented lysate.
Embodiment 3：
Control group 2, fermentation medium use single glucose carbon source：
Prepare bifidobacterium lactis tunning lysis object：
A, bifidobacterium lactis is spread cultivation in seed culture medium；
The seed culture based formulas：Glucose 30g, peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride
5g, Tween-80 1ml, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, Conyza japonica saponin(e R 0.5g,
1000ml is added water to, adjusts pH to 6.5, sterilizing is spare；
B, the bifidobacterium lactis seed to spread cultivation is seeded in fermentation medium and fermented, obtain zymotic fluid；
Fermentation medium：Glucose 2.8%, peptone 1%, acerola concentrate powder 0.5%, yeast extract 1.5%, Tween-80
0.1%th, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate 0.019%, L-cysteine hydrochloride
0.1%th, Conyza japonica saponin(e R 0.2%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0, ferment pH:5.0, speed of agitator：80r/min；
C, centrifugal treating：
By zymotic fluid centrifugal treating, centrifugal rotational speed 7000r/min, time 20min topple over supernatant collection bacterium mud；
D, it emulsifies：
According to bacterium mud quality, according to 1:5 addition Trehalosc protection agent, it is fully emulsified through high speed agitator progress, obtain breast
Change liquid；
E, it sterilizes：
Emulsion is subjected to pasteurization, obtains lactobacillus-fermented lysate.
Embodiment 4：
Test antiphlogistic effects：
10% solution of lactobacillus-fermented lysate, directly with the DMEM high glucose mediums containing 10% fetal calf serum during experiment
Required concentration is diluted to, positive control drug is hydrocortisone sodium succinate.
Experiment material
1. cell：Rat basophilic cells leukaemia cell's RBL-2H3 cells, Chinese Academy of Sciences's Shanghai life science
Institute's cell resource center；Human keratinocytes system HaCaT cells, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences's cellular resources
Center.
2. reagent：DMEM high glucose mediums and 0.25 pancreatin (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences's cellular resources
Center)；Fetal calf serum (Gibco companies)；Injection hydrocortisone sodium succinate (Biochemistry Tianjin Pharmaceutical Co)；
(Sigma is public for NaLS, Compound48/80 (C48/80) and 4- nitrobenzene-N- acetyl-β-D- glucosaminides
Department)；Histamine ELISA kit (Elabscience companies)；(Shanghai Yi Kesai biological products are limited for IL-1 α ELISA kits
Company)；Dimethyl diaminophenazine chloride (Chinese medicines group chemical reagents corporation)；CCK-8 (east Renhua Science and Technology Ltd. of Japan).
Experimental method：
HaCaT cells are stimulated using NaLS in this research, establish external inflammatory model, detect lactobacillus-fermented
The influence discharged under the concentration that lysate is acted in no cytotoxicity to the IL-1 α of NaLS induction.
Grope the suitable concentration of stimulant NaLS first.In the concentration of the μ g/mL of 3.9063 μ g/mL~62.5
Under, NaLS does not influence the proliferation of HaCaT cells with survival.And the μ g/mL lauryl sulphur of 125 μ g/mL~1000
After sour sodium effect for 24 hours, it can significantly inhibit the proliferation and vigor of HaCaT cells.Therefore, in subsequent experiment, nothing will be used
The 50 μ g/mL concentration stimulation HaCaT cells of cytotoxic effect.
It is experimentally confirmed, 25,50,100g/L lactobacillus-fermenteds lysate and 0.25,0.5,1g/L hydrocortisones
After sodium succinate acts on RBL-2H3 cells for 24 hours, there was no significant difference compared with Normal group, prompts the lactic acid bacteria of each concentration
Fermentation lysates and hydrocortisone sodium succinate are to RBL-2H3 cells without overt toxicity.
Mast cell degranulation is the basis of the pathological reactions such as anaphylactic type (hypersensitivity of I types) and inflammation.
RBL-2H3 cells are rat basophilic leukemia cell strains, have many biological characteristics of mast cell, surface expression
The mast cell of a variety of receptors and adhesion molecule and cylinder mature is quite similar, and degranulation reaction, release can occur after activation
Go out histamine, β-hexosaminidase isoreactivity substance, be the good model for evaluating allergic reaction/anaphylactoid reaction.This research
It is middle that C48/80 is used to stimulate RBL-2H3 cells as stimulant, external sensitization model is established, detects the lactobacillus-fermented of the present invention
To cell degranulation and release histamine and the shadow of β-hexosaminidase under the concentration that lysate is acted in no cytotoxicity
It rings.
Compared with Normal group cell, the IL-1 alpha levels of NaLS stimulation group cell significantly increase.It gives simultaneously
25g/L lactobacillus-fermenteds lysate or 0.25g/L hydrocortisone sodium succinates is given to can obviously reduce NaLS and lure
The IL-1 α releases led.
Specific data are as shown in the table：
It is repeated 5 times, there was no significant difference every time, has statistical significance.
As it can be seen that 25g/L lactobacillus-fermenteds lysate has significant antiphlogistic effects, the control of Conyza japonica saponin(e R and carbon source
Fixture has significant synergistic effect.
Embodiment 5：
Test antiallergic effect：
The bioactive substance (such as histamine, β-hexosaminidase) for measuring mast cell release is detection allergy/class
Allergic reaction and the common methods for evaluating drug anti-allergic effects.It is as shown in the table, compared with Normal group cell, C48/80
The histamine release of stimulation group cell significantly increases.C48/80 gives 25g/L lactobacillus-fermenteds lysate or 1g/ while stimulation
After L hydrocortisone sodium succinates, the significant reduction of histamine release level.
It is repeated 5 times, there was no significant difference every time, has statistical significance.
As it can be seen that 25g/L lactobacillus-fermenteds lysate has significant antiallergic effect, the control of Conyza japonica saponin(e R and carbon source
Fixture has significant synergistic effect.
Embodiment 6：
Electronic Speculum observes cellular morphology：
For RBL-2H3 as basophilic granulocyte, cell space is larger, full of coarse basophilic stippling in cytoplasm, there is metachromasia,
Therefore it can lead to hyperchromatic method its degranulation situation from cytomorphology.As shown in Figure 1, after neutral red staining,
Normal group cell growth is good, in fusiformis or pleomorphism, the smooth of the edge, there is complete cell membrane, is distributed into the cell
Even, in the same size, even dyeing red granules.
As shown in Fig. 2, there is the performance of degranulation in the post-stimulatory cells of C48/80：Cell rounding, swelling, cell membrane are broken
It splits, is arranged outside particle, more red dye particle is dispersed in outside cell membrane.As shown in figure 3, C48/80 gives 25g/L lactic acid while stimulation
After the processing of bacterium fermentation lysates, normal spindle cell increases, close to normal level.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (3)
1. a kind of facial mask containing bifidobacterium lactis fermentation activity extract, is prepared by the following method：
(1) bifidobacterium lactis tunning lysis object is prepared：
A, bifidobacterium lactis is spread cultivation in seed culture medium；
The seed culture based formulas：Glucose 30g, it peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride 5g, spits
- 80 1ml of temperature, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, Conyza japonica saponin(e R 0.5g, add water
To 1000ml, pH to 6.5 is adjusted, sterilizing is spare；
B, the bifidobacterium lactis seed to spread cultivation is seeded in fermentation medium and fermented, obtain zymotic fluid；
Fermentation medium：Glucose 1.8%, trehalose 1%, mannose 0.5%, peptone 1%, acerola concentrate powder 0.5%, ferment
Female cream 1.5%, Tween-80 0.1%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate 0.019%,
L-cysteine hydrochloride 0.1%, Conyza japonica saponin(e R0.2%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0, ferment pH:5.0, speed of agitator：80r/min；
C, centrifugal treating：
By zymotic fluid centrifugal treating, centrifugal rotational speed 7000r/min, time 20min topple over supernatant collection bacterium mud；
D, it emulsifies：
According to bacterium mud quality, according to 1:5 addition Trehalosc protection agent, it is fully emulsified through high speed agitator progress, obtain emulsion；
E, it sterilizes：
Emulsion is subjected to pasteurization, obtains lactobacillus-fermented lysate；
(2) prepare other pure plant components：
Prepare other plant extracting solution and additive, make facial mask liquid.
2. the facial mask described in claim 1 containing bifidobacterium lactis fermentation activity extract, it is characterised in that：
The Conyza japonica saponin(e R structural formulas are：
3. the facial mask described in claim 1 containing bifidobacterium lactis fermentation activity extract, it is characterised in that：
The seed culture based formulas：Glucose 30g, it peptone 10g, acerola concentrate powder 10g, yeast extract 5g, sodium chloride 5g, spits
- 80 1ml of temperature, dipotassium hydrogen phosphate 1g, epsom salt 0.65g, manganese sulfate monohydrate 0.19g, Conyza japonica saponin(e R 0.5g, add water
To 1000ml, pH to 6.5 is adjusted, sterilizing is spare；
The fermentation medium：Glucose 1.8%, trehalose 1%, mannose 0.5%, peptone 1%, acerola concentrate powder
0.5%th, yeast extract 1.5%, Tween-80 0.1%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.058%, manganese sulfate monohydrate
0.019%th, L-cysteine hydrochloride 0.1%, Conyza japonica saponin(e R 0.2%；
Fermentation time：9h puts tank OD：10；
Fermentation temperature：38 DEG C, control starting pH:7.0, ferment pH:5.0, speed of agitator：80r/min.
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| CN201710852338.3A CN108245479B (en) | 2017-09-19 | 2017-09-19 | Facial mask containing bifidobacterium lactis fermented active extract |
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| CN108125905A (en) * | 2018-02-18 | 2018-06-08 | 王甲林 | A kind of whitening emulsion with Lactobacillus rhamnosus fermentation activity ingredient |
| CN110638742A (en) * | 2019-11-15 | 2020-01-03 | 广州环亚化妆品科技有限公司 | Blackhead guiding composition, convergence relieving composition and blackhead removing composition consisting of blackhead guiding composition and convergence relieving composition |
| CN110801413A (en) * | 2019-12-12 | 2020-02-18 | 山东福瑞达生物科技有限公司 | Chlorella fermented extract and preparation method thereof |
| CN111494300A (en) * | 2020-05-28 | 2020-08-07 | 上海新高姿化妆品有限公司 | Cosmetic composition with comprehensive oxidation resistance and preparation method thereof |
| FR3097441A1 (en) * | 2019-06-24 | 2020-12-25 | L'oreal | Cosmetic composition comprising a lysate of Bifidobacterium species, an extract of yeast of the genus Saccharomyces, and a mannose monosaccharide and its cosmetic uses |
| CN112842987A (en) * | 2021-03-23 | 2021-05-28 | 国珍健康科技（北京）有限公司 | Whitening effect composition, preparation method and application thereof, and whitening essence |
| CN113755365A (en) * | 2021-08-11 | 2021-12-07 | 天津科技大学 | Preparation method and application of lactobacillus extract |
| CN114903839A (en) * | 2022-04-24 | 2022-08-16 | 深圳市多微生保健食品有限公司 | Facial essence containing bifidobacterium lactis fermentation product |
| CN115317432A (en) * | 2022-04-24 | 2022-11-11 | 深圳市多微生保健食品有限公司 | Facial mask containing bifidobacterium lactis fermentation product |
| CN115607495A (en) * | 2022-11-15 | 2023-01-17 | 贺松林 | Whitening acne-removing mask and preparation method thereof |
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| CN114007586A (en) * | 2019-06-24 | 2022-02-01 | 莱雅公司 | Cosmetic composition comprising lysate of bifidobacterium species, yeast extract of saccharomyces, and monosaccharide, and cosmetic use thereof |
| JP7326498B2 (en) | 2019-06-24 | 2023-08-15 | ロレアル | A cosmetic composition comprising a Bifidobacterium species lysate, a yeast extract of the genus Saccharomyces and a simple sugar and its cosmetic use |
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| CN112842987A (en) * | 2021-03-23 | 2021-05-28 | 国珍健康科技（北京）有限公司 | Whitening effect composition, preparation method and application thereof, and whitening essence |
| CN113755365A (en) * | 2021-08-11 | 2021-12-07 | 天津科技大学 | Preparation method and application of lactobacillus extract |
| CN113755365B (en) * | 2021-08-11 | 2024-02-27 | 天津科技大学 | Preparation method and application of lactobacillus extract |
| CN114903839A (en) * | 2022-04-24 | 2022-08-16 | 深圳市多微生保健食品有限公司 | Facial essence containing bifidobacterium lactis fermentation product |
| CN114903839B (en) * | 2022-04-24 | 2023-09-19 | 深圳市多微生保健食品有限公司 | Facial essence containing bifidobacterium lactis fermentation product |
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| CN115607495A (en) * | 2022-11-15 | 2023-01-17 | 贺松林 | Whitening acne-removing mask and preparation method thereof |
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