CN103952451A - Method for increasing polysaccharide content of morel submerged fermentation mycelium through astragalus extract solution - Google Patents
Method for increasing polysaccharide content of morel submerged fermentation mycelium through astragalus extract solution Download PDFInfo
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- CN103952451A CN103952451A CN201410176638.0A CN201410176638A CN103952451A CN 103952451 A CN103952451 A CN 103952451A CN 201410176638 A CN201410176638 A CN 201410176638A CN 103952451 A CN103952451 A CN 103952451A
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Abstract
The invention discloses a method for increasing polysaccharide content of morel submerged fermentation mycelium through astragalus extract solution. The method is characterized in that the astragalus extract solution is added to morel submerged fermentation culture solution to promote the growth of morel mycelium so as to increase the morel polysaccharide content. According to the method, active ingredients (alkaloid, saponin, flavones and the like) of astragalus can promote or inhibit the growth of medical fungal mycelium and the growth of metabolite.
Description
Technical field
The present invention relates to adopt Radix Astragali extractive solution to improve the method for morel submerged fermentation mycelium polysaccharides content.
Background technology
Morel (Morchella sp.) is the rare wild edible medicinal fungus of a kind of preciousness, is of high nutritive value.Modern pharmacology research shows, morel biological activity active principle food (medicine) is very high with the content of fungus polysaccharide, and its fruitbody polysaccharide is used as medicine the effect of eliminating stagnated food to destroy intestinal worms, can strengthen immune function of human body, there is antiviral, antitumor action, can reduce cholesterol level, prevent arteriosclerosis etc.
Though have and report and realize the artificial culture of morel, have no so far the report of commercialization artificial culture morel, tracing it to its cause is mainly that Morchella esculenta (L.) Pers sporophore formation stages is very responsive to air.Utilize wild sporophore to extract Morchella esculenta (L.) Pers polysaccharide cost high, raw material sources are limited.
Summary of the invention
The object of this invention is to provide a kind of method that adopts Radix Astragali extractive solution to improve morel submerged fermentation mycelium polysaccharides content.
The method that the present invention adopts Radix Astragali extractive solution to improve morel submerged fermentation mycelium polysaccharides content comprises the following steps:
A, Radix Astragali extractive solution is added in morel submerged fermentation culture medium;
B, access morel liquid spawn are to carrying out morel submerged fermentation containing in the morel submerged fermentation culture medium of Radix Astragali extractive solution, and wherein morel liquid spawn is by making in morel one-level kind access morel submerged fermentation culture medium.
Preferred version, morel submerged fermentation culture medium described in described steps A and step B consisting of by weight percentage, Semen Maydis powder 1%~5%, analysis for soybean powder 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, potassium primary phosphate 0.1%~0.2%, dipotassium hydrogen phosphate 0.1%~0.2%, all the other are water.
Preferred version, the concentration of described steps A Radix Astragali extractive solution is 5mg/ml~15mg/ml.
Preferred version, the preparation process of the Radix Astragali extractive solution in described steps A is, the Radix Astragali is soaked to 30min, then use slow fire infusion 30min, by four layers of filtered through gauze, then add water infusion one time of the dregs of a decoction is filtered, then twice filtrate is mixed, make Radix Astragali extractive solution.
Preferred version, described step B morel submerged fermentation process is, under aseptic condition, morel liquid spawn is inoculated into and has been added in astragalus extraction liquid culture medium by 10% volume ratio, 25 DEG C of temperature, pH value is 6, shaking culture in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in fermented liquid after 5-8d.The content of Morchella esculenta (L.) Pers polysaccharide reaches peak value.
Preferred version, the preparation process of morel liquid spawn is,
By in morel one-level kind aseptic technique access morel submerged fermentation culture medium, size, the thin and thick of inoculation piece float over liquid level with bacterial classification piece and do not sink and be as the criterion, and are first placed in constant incubator, 25
oc leaves standstill 48-72 hour, after mycelium germination, is placed on shaking table, with the rotating speed of 160r/min, 25
oshake-flask culture 7d at the temperature of C, makes morel liquid spawn.
Morel (Morchella sp.) one-level kind is bought in Mianyang, Sichuan Province edible mushrooms institute.
Chinese medicine astragalus (
astragalus membranaceus <Fisch.> Bunge), call continuous stilbene, be the root of leguminous herbaceous plant's Radix Astagali, Radix Astragali.The Radix Astragali is containing chemical compositions such as soap former times, flavones, amino acid, linolic acid, alkaloid, several mineral materials and VITAMIN.Activeconstituents (alkaloid, saponin(e, flavones etc.) in the Radix Astragali can promote or suppress the growth of officinal fungus mycelium and the production of meta-bolites, in morel submerged fermentation nutrient solution, add Radix Astragali extractive solution, can promote the growth of Morchella esculenta (L.) Pers mycelium, improve Morchella esculenta (L.) Pers polysaccharide content.
The present invention uses in the submerged fermentation culture medium of fungi morel at the famous and precious food in Gansu (medicine), add the Gansu Special Traditional Chinese Medicine Radix Astragali extractive solution of appropriate concentration, utilize the mutual synergism in fermenting process to improve Morchella esculenta (L.) Pers polysaccharide yield, for exploitation and the production of Morchella esculenta (L.) Pers polysaccharide provide theoretical foundation and technical support, reduce because people pluck the ecological damage causing to the transition of morel wild resource.
Embodiment
The following examples can make those skilled in the art understand better the technology, but do not limit the present invention in any way.
Embodiment 1:
1. morel submerged fermentation culture medium composition: Semen Maydis powder (liquor) 1%, analysis for soybean powder (liquor) 0.5%, magnesium sulfate 0.05%, potassium primary phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, above component is percentage composition calculating by weight all, and all the other are water.
2. the preparation of Radix Astragali extractive solution, takes Radix Astragali 250g, in 500ml water, soaks 30min, then uses slow fire infusion 30min, by four layers of filtered through gauze, then add water infusion one time of the dregs of a decoction is filtered, and then twice filtrate is mixed, and makes Radix Astragali extractive solution.
3. the interpolation of Radix Astragali extractive solution: the Radix Astragali extractive solution of 10mg/ml is added in morel submerged fermentation culture medium and amounts to 300ml, pack in the triangular flask of 500ml, cotton plug sealing, 0.12MPa sterilizing 30min, cooling rear stand-by.
4. morel liquid spawn is prepared: by morel one-level kind aseptic technique access morel submerged fermentation culture medium, size, the thin and thick of inoculation piece float over liquid level with bacterial classification piece and do not sink and be as the criterion, and are first placed in constant incubator, 25
oc leaves standstill 60 hours, after mycelium germination, is placed on shaking table, with 160r/min, 25
oc shake-flask culture 7d, makes morel liquid spawn.
5. morel submerged fermentation: extract the morel liquid spawn that 30ml prepares under aseptic condition, be inoculated into and added in astragalus extraction liquid culture medium, 25 DEG C of temperature, pH value is 6, shaking culture 5d in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in fermented liquid.
Reference examples: blank group is the submerged fermentation culture medium 300ml that does not add Radix Astragali extractive solution, packs in the triangular flask of 500ml, cotton plug sealing, and 0.12MPa sterilizing 30min, cooling rear stand-by.Access morel liquid spawn carries out morel submerged fermentation, under aseptic condition, extract the morel liquid spawn that 30ml prepares, be inoculated in blank group, 25 DEG C of temperature, pH value is 6, shaking culture 5d in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in fermented liquid.
Embodiment 1 is compared with control group, and in the fermented liquid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 25.6%, and the content of mycelium polysaccharides has improved 22.7 %.
Embodiment 2: differently from embodiment 1 be that the content of morel submerged fermentation culture medium component is different: Semen Maydis powder (liquor) 3%, analysis for soybean powder (liquor) 0.3%, magnesium sulfate 0.1%, potassium primary phosphate 0.15%, dipotassium hydrogen phosphate 0.20%, above component is percentage composition calculating by weight all, and all the other compositions are water.
Compared with control group, in the fermented liquid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 18.2%, and the content of mycelium polysaccharides has improved 8.5%.
Embodiment 3: differently from embodiment 1 be that the content of morel submerged fermentation culture medium component is different: Semen Maydis powder (liquor) 5%, analysis for soybean powder (liquor) 0.1%, magnesium sulfate 0.1%, potassium primary phosphate 0.2%, dipotassium hydrogen phosphate 0.15%, above component is percentage composition calculating by weight all, and all the other compositions are water.
Compared with control group, in the fermented liquid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 16.4%, and the content of mycelium polysaccharides has improved 6.1%.
Embodiment 4: be different from embodiment 1 adds the Radix Astragali extractive solution of 15mg/ml in morel submerged fermentation culture medium.Morel liquid spawn preparation time is 48 hours, and morel liquid spawn carries out morel submerged fermentation shaking culture 8d in constant temperature oscillator.Compared with control group, in the fermented liquid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 9.1%, and the content of mycelium polysaccharides has improved 4.9%.
Embodiment 5: be different from embodiment 1 adds the Radix Astragali extractive solution of 5mg/ml in morel submerged fermentation culture medium.Morel liquid spawn preparation time is 72 hours, and morel liquid spawn carries out morel submerged fermentation shaking culture 7d in constant temperature oscillator.Compared with control group, in the fermented liquid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 10.6%, and the content of mycelium polysaccharides has improved 7.3%.
Claims (6)
1. adopt Radix Astragali extractive solution to improve the method for morel submerged fermentation mycelium polysaccharides content, it comprises the following steps:
A, Radix Astragali extractive solution is added in morel submerged fermentation culture medium;
B, access morel liquid spawn are to carrying out morel submerged fermentation containing in the morel submerged fermentation culture medium of Radix Astragali extractive solution, and wherein morel liquid spawn is by making in morel one-level kind access morel submerged fermentation culture medium.
2. by method claimed in claim 1, it is characterized in that: the consisting of by weight percentage of the morel submerged fermentation culture medium described in described steps A and step B, Semen Maydis powder 1%~5%, analysis for soybean powder 0.1%~0.5%, magnesium sulfate 0.05%~0.1%, potassium primary phosphate 0.1%~0.2%, dipotassium hydrogen phosphate 0.1%~0.2%, all the other are water.
3. by the method described in claim 1 or 2, it is characterized in that: the concentration of described steps A Radix Astragali extractive solution is 5mg/ml~15mg/ml.
4. by method claimed in claim 3, it is characterized in that: the preparation process of the Radix Astragali extractive solution in described steps A is, the Radix Astragali is soaked to 30min, then use slow fire infusion 30min, by four layers of filtered through gauze, the infusion that again dregs of a decoction added water is filtered for one time, then twice filtrate is mixed, and makes Radix Astragali extractive solution.
5. by method claimed in claim 4, it is characterized in that: described step B morel submerged fermentation process is, under aseptic condition, morel liquid spawn is inoculated into and has been added in astragalus extraction liquid culture medium by 10% volume ratio, 25 DEG C of temperature, pH value is 6, shaking culture in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in fermented liquid after 5-8d.
6. by method claimed in claim 5, it is characterized in that: the preparation process of morel liquid spawn is, by morel one-level kind aseptic technique access morel submerged fermentation culture medium, to be first placed in constant incubator, 25
oc leaves standstill 48-72 hour, after mycelium germination, is placed on shaking table, with the rotating speed of 160r/min, 25
oshake-flask culture 7d at the temperature of C, makes morel liquid spawn.
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CN105838620A (en) * | 2016-03-28 | 2016-08-10 | 北京七巧时代科技有限公司 | Ganoderma lucidum culture medium and ganoderma lucidum culture method |
CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
CN107267403A (en) * | 2017-08-02 | 2017-10-20 | 房玉玺 | Culture medium, hickory chick compound bacteria filament and the preparation method of Morchella esculenta (L.) Pers mycelium |
CN107371801A (en) * | 2017-08-14 | 2017-11-24 | 佛山推启农业研究院(普通合伙) | A kind of artificial cultivation method for improving hickory chick unsaturated fatty acid content |
CN108070622A (en) * | 2016-11-11 | 2018-05-25 | 湖南尔康制药股份有限公司 | A kind of method that biological fermentation process prepares 1,3- propylene glycol |
CN112410229A (en) * | 2020-11-24 | 2021-02-26 | 南京林业大学 | Preparation method of toadstool mycelium rich in total flavonoids |
CN115040440A (en) * | 2022-05-20 | 2022-09-13 | 华中农业大学 | Preparation method of morchella mycelium extract and application of morchella mycelium extract as ultraviolet absorbent |
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CN105838620A (en) * | 2016-03-28 | 2016-08-10 | 北京七巧时代科技有限公司 | Ganoderma lucidum culture medium and ganoderma lucidum culture method |
CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
CN108070622A (en) * | 2016-11-11 | 2018-05-25 | 湖南尔康制药股份有限公司 | A kind of method that biological fermentation process prepares 1,3- propylene glycol |
CN107267403A (en) * | 2017-08-02 | 2017-10-20 | 房玉玺 | Culture medium, hickory chick compound bacteria filament and the preparation method of Morchella esculenta (L.) Pers mycelium |
CN107371801A (en) * | 2017-08-14 | 2017-11-24 | 佛山推启农业研究院(普通合伙) | A kind of artificial cultivation method for improving hickory chick unsaturated fatty acid content |
CN112410229A (en) * | 2020-11-24 | 2021-02-26 | 南京林业大学 | Preparation method of toadstool mycelium rich in total flavonoids |
CN115040440A (en) * | 2022-05-20 | 2022-09-13 | 华中农业大学 | Preparation method of morchella mycelium extract and application of morchella mycelium extract as ultraviolet absorbent |
CN115040440B (en) * | 2022-05-20 | 2023-06-23 | 华中农业大学 | Preparation method of Morchella mycelium extract and application of Morchella mycelium extract as ultraviolet absorbent |
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