CN103952451B - Adopt Radix Astragali extractive solution to improve the method for hickory chick submerged fermentation mycelium polysaccharides content - Google Patents
Adopt Radix Astragali extractive solution to improve the method for hickory chick submerged fermentation mycelium polysaccharides content Download PDFInfo
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- CN103952451B CN103952451B CN201410176638.0A CN201410176638A CN103952451B CN 103952451 B CN103952451 B CN 103952451B CN 201410176638 A CN201410176638 A CN 201410176638A CN 103952451 B CN103952451 B CN 103952451B
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- hickory chick
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- submerged fermentation
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Abstract
The invention discloses a kind of method that adopts Radix Astragali extractive solution to improve hickory chick submerged fermentation mycelium polysaccharides content of utilizing. The present invention, by add Radix Astragali extractive solution in hickory chick submerged fermentation nutrient solution, to promote the growth of Morchella esculenta (L.) Pers mycelium, improves Morchella esculenta (L.) Pers polysaccharide content. Active component (alkaloid, saponin(e, flavones etc.) in the Radix Astragali can promote or suppress the growth of officinal fungus mycelium and the production of metabolite.
Description
Technical field
The present invention relates to adopt Radix Astragali extractive solution to improve the method for hickory chick submerged fermentation mycelium polysaccharides content.
Background technology
Hickory chick (Morchellasp.) is the rare wild edible and medical fungi of a kind of preciousness, is of high nutritive value. Modern pharmacology research shows, hickory chick biologically active active principle food (medicine) is very high with the content of fungi polysaccharide, and its fruitbody polysaccharide is used as medicine the effect of eliminating stagnated food to destroy intestinal worms, can strengthen immune function of human body, there is antiviral, antitumor action, can reduce cholesterol level, prevent artery sclerosis etc.
Though have and report and realize the artificial cultivation of hickory chick, have no so far the report of commercialization artificial cultivation hickory chick, tracing it to its cause is mainly that Morchella esculenta (L.) Pers sporophore formation stages is very responsive to air. Utilize wild fructification to extract Morchella esculenta (L.) Pers polysaccharide cost high, raw material sources are limited.
Summary of the invention
The object of this invention is to provide a kind of method that adopts Radix Astragali extractive solution to improve hickory chick submerged fermentation mycelium polysaccharides content.
The method that the present invention adopts Radix Astragali extractive solution to improve hickory chick submerged fermentation mycelium polysaccharides content comprises the following steps:
A, Radix Astragali extractive solution is added in hickory chick submerged fermentation culture medium;
B, access hickory chick liquid spawn are to carrying out hickory chick submerged fermentation containing in the hickory chick submerged fermentation culture medium of Radix Astragali extractive solution, and wherein hickory chick liquid spawn is by making in hickory chick one-level kind access hickory chick submerged fermentation culture medium.
Preferred version, hickory chick submerged fermentation culture medium described in described steps A and step B consisting of by weight percentage, corn flour 1%～5%, analysis for soybean powder 0.1%～0.5%, magnesium sulfate 0.05%～0.1%, potassium dihydrogen phosphate 0.1%～0.2%, dipotassium hydrogen phosphate 0.1%～0.2%, all the other are water.
Preferred version, the concentration of described steps A Radix Astragali extractive solution is 5mg/ml～15mg/ml.
Preferred version, the preparation process of the Radix Astragali extractive solution in described steps A is, the Radix Astragali is soaked to 30min, then use slow fire infusion 30min, by four layers of filtered through gauze, then add water infusion one time of the dregs of a decoction is filtered, then twice filtrate is mixed, make Radix Astragali extractive solution.
Preferred version, described step B hickory chick submerged fermentation process is, under aseptic condition, hickory chick liquid spawn is inoculated into and has been added in astragalus extraction liquid culture medium by 10% volume ratio, 25 DEG C of temperature, pH value is 6, shaken cultivation in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in zymotic fluid after 5-8d. The content of Morchella esculenta (L.) Pers polysaccharide reaches peak value.
Preferred version, the preparation process of hickory chick liquid spawn is,
By in hickory chick one-level kind sterile working access hickory chick submerged fermentation culture medium, size, the thin and thick of inoculation piece float over liquid level with bacterial classification piece and do not sink and be as the criterion, and are first placed in constant incubator, 25oC leaves standstill 48-72 hour, after mycelium germination, is placed on shaking table, with the rotating speed of 160r/min, 25oAt the temperature of C, shaking flask is cultivated 7d, makes hickory chick liquid spawn.
Hickory chick (Morchellasp.) one-level kind is bought in Mianyang, Sichuan Province edible mushroom research institute.
Chinese herbal medicine astragalus (Astragalusmembranaceus<Fisch.>Bunge), call continuous stilbene, be the root of leguminous herbaceous plant's astragalus mongolicus, Astragalus membranacus. The Radix Astragali is containing chemical compositions such as soap former times, flavones, amino acid, linoleic acid, alkaloid, several mineral materials and vitamins. Active component (alkaloid, saponin(e, flavones etc.) in the Radix Astragali can promote or suppress the growth of officinal fungus mycelium and the production of metabolite, in hickory chick submerged fermentation nutrient solution, add Radix Astragali extractive solution, can promote the growth of Morchella esculenta (L.) Pers mycelium, improve Morchella esculenta (L.) Pers polysaccharide content.
The present invention uses in the submerged fermentation culture medium of fungi hickory chick at the famous and precious food in Gansu (medicine), add the Gansu Special Traditional Chinese Medicine Radix Astragali extractive solution of appropriate concentration, utilize the mutual synergistic effect in sweat to improve Morchella esculenta (L.) Pers polysaccharide yield, for exploitation and the production of Morchella esculenta (L.) Pers polysaccharide provide theoretical foundation and technical support, reduce because people pluck the ecological disruption causing to the transition of hickory chick wild resource.
Detailed description of the invention
The following examples can make those skilled in the art understand better the technology, but do not limit the present invention in any way.
Embodiment 1:
1. hickory chick submerged fermentation culture medium composition: corn flour (liquor) 1%, analysis for soybean powder (liquor) 0.5%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, above component is percentage composition calculating by weight all, and all the other are water.
2. the preparation of Radix Astragali extractive solution, takes Radix Astragali 250g, in 500ml water, soaks 30min, then uses slow fire infusion 30min, by four layers of filtered through gauze, then add water infusion one time of the dregs of a decoction is filtered, and then twice filtrate is mixed, and makes Radix Astragali extractive solution.
3. the interpolation of Radix Astragali extractive solution: the Radix Astragali extractive solution of 10mg/ml is added in hickory chick submerged fermentation culture medium and amounts to 300ml, pack in the triangular flask of 500ml, cotton plug sealing, 0.12MPa sterilizing 30min, cooling rear stand-by.
4. hickory chick liquid spawn is prepared: by hickory chick one-level kind sterile working access hickory chick submerged fermentation culture medium, size, the thin and thick of inoculation piece float over liquid level with bacterial classification piece and do not sink and be as the criterion, and are first placed in constant incubator, 25oC leaves standstill 60 hours, after mycelium germination, is placed on shaking table, with 160r/min, 25oC shaking flask is cultivated 7d, makes hickory chick liquid spawn.
5. hickory chick submerged fermentation: extract the hickory chick liquid spawn that 30ml prepares under aseptic condition, be inoculated into and added in astragalus extraction liquid culture medium, 25 DEG C of temperature, pH value is 6, shaken cultivation 5d in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in zymotic fluid.
Reference examples: blank group is the submerged fermentation culture medium 300ml that does not add Radix Astragali extractive solution, packs in the triangular flask of 500ml, cotton plug sealing, and 0.12MPa sterilizing 30min, cooling rear stand-by. Access hickory chick liquid spawn carries out hickory chick submerged fermentation, under aseptic condition, extract the hickory chick liquid spawn that 30ml prepares, be inoculated in blank group, 25 DEG C of temperature, pH value is 6, shaken cultivation 5d in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in zymotic fluid.
Embodiment 1 is compared with control group, and in the zymotic fluid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 25.6%, and the content of mycelium polysaccharides has improved 22.7%.
Embodiment 2: differently from embodiment 1 be that the content of hickory chick submerged fermentation culture medium component is different: corn flour (liquor) 3%, analysis for soybean powder (liquor) 0.3%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.15%, dipotassium hydrogen phosphate 0.20%, above component is percentage composition calculating by weight all, and all the other compositions are water.
Compared with control group, in the zymotic fluid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 18.2%, and the content of mycelium polysaccharides has improved 8.5%.
Embodiment 3: differently from embodiment 1 be that the content of hickory chick submerged fermentation culture medium component is different: corn flour (liquor) 5%, analysis for soybean powder (liquor) 0.1%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.2%, dipotassium hydrogen phosphate 0.15%, above component is percentage composition calculating by weight all, and all the other compositions are water.
Compared with control group, in the zymotic fluid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 16.4%, and the content of mycelium polysaccharides has improved 6.1%.
Embodiment 4: be different from embodiment 1 adds the Radix Astragali extractive solution of 15mg/ml in hickory chick submerged fermentation culture medium. Hickory chick liquid spawn preparation time is 48 hours, and hickory chick liquid spawn carries out hickory chick submerged fermentation shaken cultivation 8d in constant temperature oscillator. Compared with control group, in the zymotic fluid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 9.1%, and the content of mycelium polysaccharides has improved 4.9%.
Embodiment 5: be different from embodiment 1 adds the Radix Astragali extractive solution of 5mg/ml in hickory chick submerged fermentation culture medium. Hickory chick liquid spawn preparation time is 72 hours, and hickory chick liquid spawn carries out hickory chick submerged fermentation shaken cultivation 7d in constant temperature oscillator. Compared with control group, in the zymotic fluid of interpolation Radix Astragali extractive solution, the dry weight of Morchella esculenta (L.) Pers mycelium has improved 10.6%, and the content of mycelium polysaccharides has improved 7.3%.
Claims (4)
1. adopt Radix Astragali extractive solution to improve the method for hickory chick submerged fermentation mycelium polysaccharides content, it comprises the following steps:
A, Radix Astragali extractive solution is added in hickory chick submerged fermentation culture medium;
B, access hickory chick liquid spawn are to carrying out hickory chick submerged fermentation containing in the hickory chick submerged fermentation culture medium of Radix Astragali extractive solution, and wherein hickory chick liquid spawn is by making in hickory chick one-level kind access hickory chick submerged fermentation culture medium;
Hickory chick submerged fermentation culture medium described in described steps A and step B consisting of by weight percentage, corn flour 1%～5%, analysis for soybean powder 0.1%～0.5%, magnesium sulfate 0.05%～0.1%, potassium dihydrogen phosphate 0.1%～0.2%, dipotassium hydrogen phosphate 0.1%～0.2%, all the other are water;
The preparation process of the Radix Astragali extractive solution in described steps A is, the Radix Astragali is soaked to 30min, then uses slow fire infusion 30min, by four layers of filtered through gauze, then the dregs of a decoction infusion that adds water filtered for one time, then twice filtrate mixed, and makes Radix Astragali extractive solution.
2. method according to claim 1, is characterized in that: the concentration of described steps A Radix Astragali extractive solution is 5mg/ml～15mg/ml.
3. method according to claim 1 and 2, it is characterized in that: described step B hickory chick submerged fermentation process is, under aseptic condition, hickory chick liquid spawn is inoculated into and has been added in astragalus extraction liquid culture medium by 10% volume ratio, 25 DEG C of temperature, pH value is 6, shaken cultivation in the constant temperature oscillator of rotating speed 160r/min, measures Morchella esculenta (L.) Pers mycelium dry weight and mycelium polysaccharides content in zymotic fluid after 5-8d.
4. method according to claim 3, is characterized in that: the preparation process of hickory chick liquid spawn is, by hickory chick one-level kind sterile working access hickory chick submerged fermentation culture medium, to be first placed in constant incubator, 25oC leaves standstill 48-72 hour, after mycelium germination, is placed on shaking table, with the rotating speed of 160r/min, 25oAt the temperature of C, shaking flask is cultivated 7d, makes hickory chick liquid spawn.
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| CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
| CN108070622A (en) * | 2016-11-11 | 2018-05-25 | 湖南尔康制药股份有限公司 | A kind of method that biological fermentation process prepares 1,3- propylene glycol |
| CN107267403A (en) * | 2017-08-02 | 2017-10-20 | 房玉玺 | Culture medium, hickory chick compound bacteria filament and the preparation method of Morchella esculenta (L.) Pers mycelium |
| CN107371801A (en) * | 2017-08-14 | 2017-11-24 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick unsaturated fatty acid content |
| CN112410229B (en) * | 2020-11-24 | 2022-04-05 | 南京林业大学 | Preparation method of toadstool mycelium rich in total flavonoids |
| CN115040440B (en) * | 2022-05-20 | 2023-06-23 | 华中农业大学 | Preparation method of Morchella mycelium extract and application of Morchella mycelium extract as ultraviolet absorbent |
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