CN101810242A - Micro-ecological preparation for feed and preparation method thereof - Google Patents
Micro-ecological preparation for feed and preparation method thereof Download PDFInfo
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- CN101810242A CN101810242A CN200910073123A CN200910073123A CN101810242A CN 101810242 A CN101810242 A CN 101810242A CN 200910073123 A CN200910073123 A CN 200910073123A CN 200910073123 A CN200910073123 A CN 200910073123A CN 101810242 A CN101810242 A CN 101810242A
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Abstract
The invention provides a micro-ecological preparation for a feed and a preparation method thereof. The method comprises the following steps of: firstly, performing liquid state fermentation on primary and secondary seeds by using 5 strains, namely lactobacillus plantarum, lactococcus lactis, lactobacillus rhamnosus, aspergillus niger and saccharomycopsis fibuligera; secondly, uniformly mixing high temperature soybean meal, wheat bran, corn meal and rice hulls in a weight ratio of (20-25):(50-60):(18-20):(3-5); adding 18 percent solution of urea in an amount which is 15 to 20 weight percent of the mixture into the mixture; inoculating liquid secondary seeds in an amount which is 5 percent of a humid weight of the solid mixture; uniformly mixing the mixture; flattening the solid mixture to a thickness of 12 to 20cm; culturing the solid mixture at the temperature of 30 DEG C for 48 hours; performing ventilation drying on the cultured solid mixture at the temperature of between 40 and 45 DEG C and stopping drying till the moisture content of the solid mixture is less than 5 percent; and crushing and packaging the solid mixture to obtain the finished products.
Description
(1) technical field
The present invention relates to a kind of feed biologic product, also relate to the preparation method of this biologic product.
(2) background technology
So-called feed probiotics is to adopt known useful microorganism to mix the feed microbial inoculum of safe, pollution-free, the noresidue that contains active probiotic of making through special process such as fermentation, dryings with nutrition carrier under little ecological theory instructs.Britain's " rabid ox disease " incident and Belgium's " dioxin " incident that successively took place in the world in 2000 have caused the people of other countries' fear, cause sharp pounding in the Continental Europe, and the consumer serious crisis occurs to the consumption confidence of livestock products.People begin to call strongly green feed, propose slogans such as " creating healthy food with healthy feed ", " feed is human direct food ", and the safety problem of feed has caused the great attention of countries in the world government.At above-mentioned condition, the development of biological feedstuff is grown in strength year by year, the various effects of its product also showing in various degree in practice is cultured, and its purposes also develops into aquaculture by original breeding green livestock and poultry, and purposes is more and more widely.
In China is production of fodder big country in the world, and 10,000 5 thousand tame production of fodder producers are arranged.Recent statistics result in 2007,2,500 hundred million yuan of China's feed gross output values, output breaks through 100,000,000 tons of high pointes first, has reached 1.05 hundred million tons, is sure to occupy the second place of the world.According to statistics, the whole nation year produces 2,000,000 tons of various biological feedstuffs, and abroad compares, and the biological feedstuff kind of China is few, output is little, price is high, and has a lot of products not form large-scale production as yet, temporarily can not satisfy the needs of aquaculture production development.China's liquid phase process manufacture order cell protein cumulative production less than is 10,000 tons so far, about 300,000 tons of the solid state fermentation Yeast protein feed year of circulating on the market, compare for 1,000 ten thousand tons with market demand and to differ greatly, in view of the above, China removes and has issued " feed and feed addictive management rules " in 1999, reinforcement is raised work with ruling by law, outside the assurance feed safety, feed research institution of country is in the measure that proposes 21 century China's feed industry sustainable development, emphasis is mentioned 21 century and is utilized biotechnology, reinforcement is to the exploitation of environment-friendly type and clean type feed, thus 21 century biological feedstuff vast potential for future development will be arranged.
(3) summary of the invention
The object of the invention is to provide a kind of feed probiotics that can improve the livestock and poultry immunity of organisms, reduce ight soil stink environmental pollution, and the object of the invention also is to provide the preparation method of this probiotics.
The object of the present invention is achieved like this: related percentage is mass ratio except that other has indicating among the present invention, and product of the present invention adopts such method to prepare:
1. the preparation of the selection of fermentation strain and culture medium
A bacterium---Lactobacillus plantarum CLCC6051, culture medium add yeast extract 0.5%, aseptic calcium carbonate 0.6% in 5 ° of B é brewer's worts.
B bacterium---Lactococcus lactis CICC6029, culture medium are beef extract 0.5%, yeast extract 0.5%, peptone 1%, glucose 1%, lactose 0.5%, sodium chloride 0.5%, PH6.8.
C bacterium---Lactobacillus rhamnosus CICC6141, culture medium are peptone 10 grams, beef extract 10 grams, yeast extract 5 grams, glucose 20 grams, dipotassium hydrogen phosphate 2 grams, sodium acetate 5 grams, ammonium citrate 2 grams, magnesium sulfate 0.2 gram, manganese sulfate 0.2 gram, 1 milliliter of Tween 80,1000 milliliters in water, pH6.2-6.6.
D bacterium---aspergillus niger CICC40700, culture medium are potato 200 grams of removing skin, are cut into small pieces, add 1000 milliliters in water and boiled 30 fens, and clock, the elimination potato ball complements to 1000 milliliters with filtrate, adds glucose 20 grams.
E bacterium---saccharomycopsis fibuligera CICC1717, culture medium are 5 ° of B é brewer's worts.
2. the liquid sweat of bacterial classification
2.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10ml respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, the freeze-dried vaccine powder in the 5 strain bacterium peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
2.2 enlarging, cultivates liquid bacterial classification
2.2.1 the preparation of first order seed
Measure 5 each 200ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% of culture volume and inoculated activated bacterial classification in 2.1 steps, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as first order seed.
2.2.2 the preparation of secondary seed
Measure 5 each 2000ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% inoculation first order seed of culture volume, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as secondary seed.
3. strains solid fermented process
According to weight ratio is the high temperature dregs of beans: wheat bran: corn flour: rice husk=(20-25): (50-60): (18-20): ratio (3-5) mixes and obtains solid mixture, in solid mixture, add the urea liquid that accounts for solid mixture weight 15-20%, urea concentration is 18%, under 100-105 ℃ of temperature, steam 20-30 minute, be cooled to 30-35 ℃, press the liquid secondary seed of 5% inoculation of solid mixt weight in wet base, mix, then this solid mixt is shakeout, thickness 12-20cm cultivated 48 hours for 30 ℃.
4. dry run
With the aeration-drying under 40-45 ℃ of condition of cultured solid mixt, moisture is lower than 5% in solid mixt, can stop drying, and crushing packing is finished product then.
3. effect
Prove the effect of patent of the present invention below by concrete experiment
(1). experimental animal is selected:
Select each 20 of wean one all piglets, be divided into two groups, establish test group and control group.
(2). test method:
Weaning begins to enter preliminary trial period the last week, weans and weighs in back second day, and grouping enters experimental period.
(3). feed form and feed formula: adopt the particulate material free choice feeding, addition 10% adds after eating up immediately, can not fracture.
Control group: popcorn 48%, expanded soybean 17.5%, whole milk powder 10%, whey powder 7%, swelling soya dreg 4%, fish meal 5.5%, sucrose 3%, additive 5%.
Test group: popcorn 48%, expanded soybean 17.5%, whole milk powder 10%, whey powder 7%, microecological microbial agent 4%, fish meal 5.5%, sucrose 3%, additive 5%.
(4). result of the test (10 days)
| Just average heavy Kg | The average heavy Kg in end | Average daily gain g | The material anharmonic ratio | The diarrhoea ratio | Sick ratio | Admittedly give up the smell evaluation |
| Control group 9.15 | ??12.7 | ??355 | ??1.28∶1 | ??1∶10 | ??2∶10 | Stink is heavy |
| Test group 8.95 | ??14.6 | ??565 | ??1.2∶1 | Do not have | Do not have | Stink is light |
(5). interpretation of result
Product of the present invention can effectively reduce the diarrhea rate of piglet, reduces sick ratio, alleviates the stink of discharging ight soil, improves to increase day by day weight more than 37%.
(5) specific embodiment
For a more detailed description to the present invention for example below:
Embodiment one
5 probiotics that the present invention adopts are all purchased in Chinese industrial microorganism fungus kind preservation administrative center, be respectively: Lactobacillus plantarum CICC6051, Lactococcus lactis CICC6029, Lactobacillus rhamnosus CICC6141, aspergillus niger CICC40700, saccharomycopsis fibuligera CICC1717.
1. the liquid sweat of bacterial classification
1.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10ml respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, the freeze-dried vaccine powder in the 5 strain bacterium peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
1.2 enlarging, cultivates liquid bacterial classification
1.2.1 the preparation of first order seed
Measure 5 each 200ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% of culture volume and inoculated activated bacterial classification in 2.1 steps, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as first order seed.
1.2.2 the preparation of secondary seed
Measure 5 each 2000ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% inoculation first order seed of culture volume, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as secondary seed.
2. strains solid fermented process
According to weight ratio is the high temperature dregs of beans: wheat bran: corn flour: rice husk=(20-25): (50-60): (18-20): ratio (3-5) mixes and obtains solid mixture, in solid mixture, add the urea liquid that accounts for solid mixture weight 15-20%, urea concentration is 18%, under 100-105 ℃ of temperature, steam 20-30 minute, be cooled to 30-35 ℃, press the liquid secondary seed of 5% inoculation of solid mixt weight in wet base, mix, then this solid mixt is shakeout, thickness 12-20cm cultivated 48 hours for 30 ℃.
3. dry run
With the aeration-drying under 40-45 ℃ of condition of cultured solid mixt, moisture is lower than 5% in solid mixt, can stop drying, and crushing packing is finished product then.
Embodiment two
1. the liquid sweat of bacterial classification
1.1 the activation of freeze-dried vaccine powder
Measure 0.9% physiological saline 10ml respectively in vitro in 5, be cooled to 30 ℃ in 20 minutes through 121 ℃ of sterilizations, the freeze-dried vaccine powder in the 5 strain bacterium peace bottle all poured under germ-free condition in 0.9% the physiological saline, concussion makes its dissolving, static activation is 30 minutes in 30 ℃ of insulating boxs, and is standby.
1.2 enlarging, cultivates liquid bacterial classification
1.2.1 the preparation of first order seed
Measure 5 each 200ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% of culture volume and inoculated activated bacterial classification in 2.1 steps, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as first order seed.
1.2.2 the preparation of secondary seed
Measure 5 each 2000ml of strain bacterium culture medium respectively in 5 500ml triangular flasks, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, pressed 10% inoculation first order seed of culture volume, cultivated 24 hours at 30 ℃ of shaking tables, shaking speed 120-130 rev/min, as secondary seed.
2. strains solid fermented process
According to weight ratio is the high temperature dregs of beans: wheat bran: corn flour: rice husk=22: 55: 19: 4 ratio mixes and obtains solid mixture, in solid mixture, add the urea liquid that accounts for solid mixture weight 15-20%, urea concentration is 18%, under 100-105 ℃ of temperature, steam 20-30 minute, be cooled to 30-35 ℃, press the liquid secondary seed of 5% inoculation of solid mixt weight in wet base, mix, then this solid mixt is shakeout, thickness 12-20cm cultivated 48 hours for 30 ℃.
3. dry run
With the aeration-drying under 40-45 ℃ of condition of cultured solid mixt, moisture is lower than 5% in solid mixt, can stop drying, and crushing packing is finished product then.
Claims (2)
1. feed probiotics and preparation method thereof is characterized in that: preparation Lactobacillus plantarum, Lactococcus lactis, Lactobacillus rhamnosus, aspergillus niger, the seed liquor of saccharomycopsis fibuligera 5 strain bacterium is inoculated in the solid mixt of high temperature dregs of beans, wheat bran, corn flour, rice husk.
2. a kind of feed probiotics according to claim 1 and preparation method thereof, it is characterized in that: the ratio of solid mixt is: high temperature dregs of beans: wheat bran: corn flour: rice husk=(20-25): (50-60): (18-20): (3-5), add the urea liquid of mixture weight 15-20% again, urea concentration is 18%.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102524586A (en) * | 2012-02-03 | 2012-07-04 | 天津赛普瑞斯科技有限公司 | Prebiotics polysaccharide feed and production method thereof |
| CN102559546A (en) * | 2011-12-15 | 2012-07-11 | 李绩 | Composite microbial leaven |
| CN102578441A (en) * | 2011-12-15 | 2012-07-18 | 李绩 | Method for producing silage using compound microorganism fermentation straws |
| CN102599335A (en) * | 2012-03-07 | 2012-07-25 | 哈尔滨市海澳斯生物科技开发有限公司 | Method for preparing compound microorganism fermented forage feed |
| CN103518979A (en) * | 2012-07-02 | 2014-01-22 | 马万龙 | Organic livestock and egg feed rich in linolenic acid, and feeding method of feed |
| CN104012754A (en) * | 2014-06-10 | 2014-09-03 | 寿阳华阳农牧有限公司 | Feed rich in lactococcus lactis and preparation method thereof |
| CN104605138A (en) * | 2013-11-01 | 2015-05-13 | 南京黑玉特种家禽养殖专业合作社 | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof |
| CN107927418A (en) * | 2017-11-27 | 2018-04-20 | 上海创博生态工程有限公司 | A kind of additive for microbe feedstuff and preparation method for improving immunity of layer chicken |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
| CN112335606A (en) * | 2020-11-11 | 2021-02-09 | 贵州省畜牧兽医研究所 | Isolated weaning feeding method for local Guizhou piglets |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102578441B (en) * | 2011-12-15 | 2013-09-25 | 宁夏盐池西部畅享农业生物科技有限公司 | Method for producing silage using compound microorganism fermentation straws |
| CN102559546A (en) * | 2011-12-15 | 2012-07-11 | 李绩 | Composite microbial leaven |
| CN102578441A (en) * | 2011-12-15 | 2012-07-18 | 李绩 | Method for producing silage using compound microorganism fermentation straws |
| CN102559546B (en) * | 2011-12-15 | 2013-07-24 | 李绩 | Composite microbial leaven |
| CN102524586A (en) * | 2012-02-03 | 2012-07-04 | 天津赛普瑞斯科技有限公司 | Prebiotics polysaccharide feed and production method thereof |
| CN102599335B (en) * | 2012-03-07 | 2013-10-30 | 哈尔滨市海澳斯生物科技开发有限公司 | Method for preparing compound microorganism fermented forage feed |
| CN102599335A (en) * | 2012-03-07 | 2012-07-25 | 哈尔滨市海澳斯生物科技开发有限公司 | Method for preparing compound microorganism fermented forage feed |
| CN103518979A (en) * | 2012-07-02 | 2014-01-22 | 马万龙 | Organic livestock and egg feed rich in linolenic acid, and feeding method of feed |
| CN103518979B (en) * | 2012-07-02 | 2016-05-25 | 马万龙 | Be rich in the organic fowl poultry of leukotrienes and eggs feed and feeding method |
| CN104605138A (en) * | 2013-11-01 | 2015-05-13 | 南京黑玉特种家禽养殖专业合作社 | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof |
| CN104012754A (en) * | 2014-06-10 | 2014-09-03 | 寿阳华阳农牧有限公司 | Feed rich in lactococcus lactis and preparation method thereof |
| CN107927418A (en) * | 2017-11-27 | 2018-04-20 | 上海创博生态工程有限公司 | A kind of additive for microbe feedstuff and preparation method for improving immunity of layer chicken |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
| CN112335606A (en) * | 2020-11-11 | 2021-02-09 | 贵州省畜牧兽医研究所 | Isolated weaning feeding method for local Guizhou piglets |
| CN112335606B (en) * | 2020-11-11 | 2022-07-01 | 贵州省畜牧兽医研究所 | Isolated weaning feeding method for local Guizhou piglets |
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Application publication date: 20100825 |