CN102578441A - Method for producing silage using compound microorganism fermentation straws - Google Patents
Method for producing silage using compound microorganism fermentation straws Download PDFInfo
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- CN102578441A CN102578441A CN2011104219692A CN201110421969A CN102578441A CN 102578441 A CN102578441 A CN 102578441A CN 2011104219692 A CN2011104219692 A CN 2011104219692A CN 201110421969 A CN201110421969 A CN 201110421969A CN 102578441 A CN102578441 A CN 102578441A
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- 238000000855 fermentation Methods 0.000 title abstract description 17
- 230000004151 fermentation Effects 0.000 title abstract description 17
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 239000004460 silage Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims description 30
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 30
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing silage using compound microorganism fermentation straws. The method includes that a leavening agent is added into a silage raw material, microorganisms grow rapidly under the aerobic and anaerobic conditions, a large quantity of cellulase is secreted to decompose cellulose, protein is decomposed by protease to generate free amino acids, and simultaneously a large quantity of metabolic products of lactic acids and the like are generated. Potential of hydrogen (pH) value of the processed silage is decreased, and sundry fungi are basically absent, thereby the silage is high in nutritional value, easy to digest and applicable to livestock.
Description
Technical field
The invention belongs to bacterial classification and mixed bacterium leavening agent thereof in the additive for microbe feedstuff field, the invention still further relates to and utilize this mixed bacterium leavening agent to produce the method for greenfeed.
Background technology:
The stalk resource of China is very abundant, and annual production can reach 5.79 hundred million tons.Wherein, corn, wheat and rice straw three big crop material output have reached 4.39 hundred million tons, account for 75.88% of whole stalk output.At present,, aggravated the dependence of animal husbandry, but even stalk also improper because of processing and utilization as feed, makes that the utilization rate of stalk and price of deed rate are low as the feed negligible amounts to grain along with the fast development of animal husbandry.Agricultural crop straw is of low nutritive value, palatability is poor, digestibility is low.If it is reasonably processed adjustment, can be used for the domestic animal of feeding.It is reported that stalk is after the medical treatment of methods such as ensiling, ammonification, every 6kg ensiling stalks or 4kg ammoniated straw are equivalent to the nutritive value of 1kg corn feed.Therefore, adopt scientific methods that stalk is processed adjustment, be widely used in animal husbandry, have good ecological benefits social benefit and economic benefit.
The ammoniated forage of promoting in China in recent years, not only cost is high, energy consumption is high, and existence and agricultural strive fertilizer, contaminated environment, and some method is for livestock toxic side effect also.And feed is known Study on Technology and is used the existing century-old history that, and crucial at present technology is the optimization of the effective composite and zymotechnique of the seed selection of microorganism strain excellent, composite bacteria.
In these areas; Chinese scholars has all been carried out number of research projects, but for comprehensive solution know suppress in the feed fermentation process spoilage organisms, the control secondary fermentation, effectively control organic acid and lignocellulose degradation; Reduce cost, aspect such as easy to use still has problems.
Summary of the invention:
To the problem of present existence, the object of the present invention is to provide a kind of greenfeed to produce the method for greenfeed with microbe leaven.
The technology that the blue or green storage of microorganism mixed bacterium leavening agent is made greenfeed:
1) raw material is prepared: straw, cornstalk and pulse family class bar raw material or its mixture that selected nothing is rotted, hand hay cutter is long to 2～5cm.
Actication of culture: the 100g compound microbial culture starter is poured in 1～2 ° of Brix brewer's wort, mixed, 25 ℃～35 ℃ was that activation is good through 2～3 hours down.The water content of raw material (straw, cornstalk and pulse family class bar raw material or its mixture) should be controlled at 70%～80%, and the compound microbial culture starter consumption is the 0.1-5% of raw material.
2) inoculation charging: the raw material layer, when accumulation 20～30cm is thick, spray one time bacterium liquid.Piling up height overall should be above 2 meter.
3) aerobic cultivation: placed in the air 5～30 days, it is 20～30 days that spring, two seasons of autumn are answered the proper extension time; Placed summer 5～10 days.
4) anaerobism is cultivated: with the plastic sheeting sealing, can be edible after 20～90 days for livestock, and spring, Qiu Liangji time are 60～90 days.20～30 days summers.
The present invention produces with compound microbial culture starter aspergillus niger spore, Lactobacillus rhamnosus CGMCC4430 and Lactobacillus plantarum and forms.Each bacterial classification viable count weight range is following in the compound microbial culture starter: Lactobacillus rhamnosus is 109 of (0.1～60.0) */gram, and Lactobacillus plantarum quantity is 109 of (0.01～6.0) */gram, and aspergillus niger spore quantity is 108 of (1～50) */gram.
It is 1 with mass ratio that fermentation is made aspergillus niger spore, Lactobacillus rhamnosus powder, Lactobacillus plantarum powder: (0.5-2): (0.5-2) mix, with dry weight basis.
The compound microbial culture starter consumption is the 0.1-1% of raw material.
5) preparation technology of aspergillus niger spore is:
A. inclined-plane cultural method
Test tube, 121 ℃, after the 20min sterilization, pendulum inclined-plane, cooling, inoculation.Cultivate the black spore for 30 ℃ and be paved with the inclined-plane.
B.K formula blake bottle spore
Get 10 ° of Brix brewer's worts and add 2% agar, the 500mL K formula of packing into blake bottle, 121 ℃, after the 20min sterilization, the cooling of inclined-plane, shop.Insert spore suspension 1mL, guarantee that suspension is inoculated in whole media surface; Be sidelong into insulating box, cultivate the black spore for 30 ℃ and be paved with the inclined-plane.
C. solid-state amplification culture
K formula phialosporae is processed spore suspension, and spore concentration is greater than 1.0 * 10
8Individual/mL.Get 200kg solid medium (wheat bran 140kg, 10 ° of Brix brewer's wort 60L), fully put into tray behind the mixing, sterilized 1 hour down at 121 ℃.After waiting to cool, insert spore suspension.Cultivation temperature is controlled at 30 ℃, humidity 80-90%, whenever once at a distance from 10 hours stirrings, incubation time 3 days; Treat that compost covers with spore and can finish to cultivate
Drying and crushing: after the fermentation ends, tray is placed on fluidized bed drying, baking temperature is controlled at 60 ℃, treats that moisture content of material will be lower than 10% when following, with pulverizer solid culture medium is pulverized, and material is pulverized the aperture more than 100 orders.
6) preparation technology of Lactobacillus rhamnosus and Lactobacillus plantarum bacterium powder is:
Lactobacillus plantarum, Lactobacillus rhamnosus powdery bacterium powder production stage are following: the volume that slant strains is transferred to fluid nutrient medium and spreads cultivation step by step and require; The bacterium liquid that obtains spreading cultivation centrifugalizes, the collecting precipitation thalline; In the deposition thalline, add protective agent and dilute; Utilize drying equipment to prepare the powdery microbial inoculum, composite bacterium powder can add the proper ratio of starch and each bacterium viable count of dextrin realization when mixing.Each bacterial classification viable count weight range is following in the final bacterium powder product: Lactobacillus rhamnosus is (0.1～60.0) * 10
9Individual/gram, Lactobacillus plantarum quantity is (0.01～6.0) * 10
9Individual/gram, aspergillus niger spore quantity is (1～50) * 10
8Individual/gram.
The used culture medium of Lactobacillus rhamnosus is following: slant medium (g/L): 20 glucose, 5 dusty yeasts, 10 soy peptones, 10 beef extracts, 5NaCl, 10 sodium acetates, 2 ammonium citrates, 0.2MgSO
47H
2O, 0.05MnSO
47H
2O, 15 agar, pH 6.5.
Fermentation medium (g/L): 40 glucose, 10 dusty yeasts, 10 soy peptones, inorganic salts (0.01NaCl, 0.5 sodium acetate, 0.2 ammonium citrate, 0.2KH
2PO
4, 0.2MgSO
47H
2O, 0.05MnSO
47H
2O), pH 6.5.
Various bacterial classification proportion of composing also are to obtain through meticulous experimental study among the present invention, and the selection of above-mentioned bacterial classification and proportioning have ensured the good quality of product.
Composite bacterium powder can add the proper ratio of starch and each bacterium viable count of dextrin realization when mixing.
The better scope of each bacterial classification living cells quantity is following in the composite bacterium powder: Lactobacillus rhamnosus is (1～60.0) * 10
9Individual/gram, Lactobacillus plantarum quantity is (0.1～6.0) * 10
9Individual/gram, aspergillus niger spore quantity is (1～30) * 10
8Individual/gram.
The concrete production method of Lactobacillus rhamnosus bacterium powder has more report; The report article has the master thesis " vacuum freeze-drying method is produced the research of dry acid milk leavening technology " (2002) of Huang Liangchang, and " development of directly use type ferment agent for sour milk " that Liu Yufeng etc. deliver at Chinese dairy industry magazine is published in the 5th phase of nineteen ninety-five.
The bacterial classification that the present invention adopts is following:
Lactobacillus rhamnosus (Lactob8cillus rhamnosus) CGMCC No.4430 bacterial strain characteristics are following: examine under a microscope, this bacterial strain is shaft-like, and width is less than 1 μ m, and 2 to 3 bacillus are easy to be linked to be and link together; On solid medium, this bacterium bacterium colony is a milky, and smooth surface is moistening, thickness, and the edge is more neat.Compare with original bacterium, this mutagenic strain is significantly less than starting strain on form.Starting strain Lactobacillus rhamnosus CGMCC No.1.2134 purchases in China Committee for Culture Collection of Microorganisms common micro-organisms center.Lactobacillus rhamnosus of the present invention adopts following flow process to carry out seed selection: a bottle multiple sieve → mitotic stability test → 5L fermentation tank test is screened → shaken to the original bacterial classification that sets out → test tube activation → high temperature acclimation → dithyl sulfate (DES) mutagenesis → high sugared plate screening → nitrosoguanidine (NTG) mutagenesis screening → high temperature bacterium.Purpose bacterial strain CGMCC No.4430 is done the experiment of 5L lactic fermentation jar, and the result shows: compare with starting strain, CGMCC No.4430 glucose tolerance concentration can reach 270g/L, compares with original bacterium and has improved 95%; After the fermentation ends, lactic acid content is 60g/L, compares with original bacterium and has improved 158%.
Lactobacillus rhamnosus (Lactobacillus rhamnosus) CGMCC No.4430 depositary institution is a Chinese common micro-organisms culture presevation administrative center.Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101.Preservation date on December 8th, 2010.
Aspergillus niger (Aspergillus niger) CGMCC No.3.5269, Lactobacillus plantarum (Lactobacillus plantarum CGMCC.No.1.2158) are purchased in Chinese common micro-organisms culture presevation administrative center.
Beneficial effect:
Through aspergillus niger, Lactobacillus rhamnosus, Lactobacillus plantarum are formed the high activity compound microbial culture starter.And special greenfeed is fermented is divided into aerobic and two stages of anaerobism.Promoted the quick growth of thalline like this at aerobic stage, anaerobic stages is beneficial to the quick accumulation of metabolin, and the present invention has effectively solved the technical barrier in the greenfeed fermentation, uses simply, is convenient to the peasant and uses on a large scale.
The specific embodiment: following through utilizing the compound microorganism ferments stalk to produce the method for greenfeed among concrete embodiment narration the present invention.Unless stated otherwise, used technological means is method known in those skilled in the art among the present invention.In addition, embodiment is interpreted as illustrative, and unrestricted scope of the present invention, essence of the present invention and scope are only limited claims.To those skilled in the art, under the prerequisite that does not deviate from essence of the present invention and scope, various changes or change that the nutrient chemical component in these embodiments, content, condition of culture are carried out also belong to protection scope of the present invention.
Embodiment 1: the preparation method of mixed culture fermentation agent
1) preparation technology of aspergillus niger is:
A. inclined-plane cultural method
Test tube, 121 ℃, after the 20min sterilization, pendulum inclined-plane, cooling, inoculation.Cultivate the black spore for 30 ℃ and be paved with the inclined-plane.
B.K formula blake bottle spore
Get 10 ° of Brix brewer's worts and add 2% agar, the 500mL K formula of packing into blake bottle, 121 ℃, after the 20min sterilization, the cooling of inclined-plane, shop.Insert spore suspension 1mL, guarantee that suspension is inoculated in whole media surface; Be sidelong into insulating box, cultivate the black spore for 30 ℃ and be paved with the inclined-plane.
C. solid-state amplification culture
K formula phialosporae is processed spore suspension, and spore concentration is greater than 1.0 * 10
8Individual/mL.Get 200kg solid medium (wheat bran 140kg, 10 ° of Brix brewer's wort 60L), fully put into tray behind the mixing, sterilized 1 hour down at 121 ℃.After waiting to cool, insert spore suspension.Cultivation temperature is controlled at 30 ℃, humidity 80-90%, whenever once at a distance from 10 hours stirrings, incubation time 3 days; Treat that compost covers with spore and can finish to cultivate
Drying and crushing: after the fermentation ends, tray is placed on fluidized bed drying, baking temperature is controlled at 60 ℃, treats that moisture content of material will be lower than 10% when following, with pulverizer solid culture medium is pulverized, and material is pulverized the aperture more than 100 orders.
The preparation technology of Lactobacillus rhamnosus CGMCC4430 and Lactobacillus plantarum (Lactobacillusplantarum CGMCC.No.1.2158) bacterium powder is: Lactobacillus plantarum, Lactobacillus rhamnosus powdery bacterium powder production stage are following: the volume that slant strains is transferred to fluid nutrient medium and spreads cultivation step by step and require; The bacterium liquid that obtains spreading cultivation centrifugalizes, the collecting precipitation thalline; In the deposition thalline, add protective agent and dilute; Utilize drying equipment to prepare the powdery microbial inoculum, said method makes that viable count is (0.1～6.0) * (10 in the Lactobacillus rhamnosus bacterium powder
10) individual/gram, viable count is (0.1～6.0) 10 in the Lactobacillus plantarum bacterium powder
9Individual/gram, composite bacterium powder can add the proper ratio of starch and each bacterium viable count of dextrin realization when mixing.Each bacterial classification viable count weight range is following in the final bacterium powder product: Lactobacillus rhamnosus is (0.1～60.0) * 10
9Individual/gram, Lactobacillus plantarum quantity is (0.01～6.0) * 10
9Individual/gram, aspergillus niger spore quantity is (1～50) * 10
8Individual/gram.
The used culture medium of Lactobacillus rhamnosus is following: slant medium (g/L): 20 glucose, 5 dusty yeasts, 10 soy peptones, 10 beef extracts, 5NaCl, 10 sodium acetates, 2 ammonium citrates, 0.2MgSO
47H
2O, 0.05MnSO
47H
2O, 15 agar, pH 6.5.
Fermentation medium (g/L): 40 glucose, 10 dusty yeasts, 10 soy peptones, inorganic salts (0.01NaCl, 0.5 sodium acetate, 0.2 ammonium citrate, 0.2KH
2PO
4, 0.2MgSO
47H
2O, 0.05MnSO
47H
2O), pH 6.5.
Embodiment 2:
200g aspergillus niger spore, 100g Lactobacillus rhamnosus and 100g Lactobacillus plantarum that fermentation is made mix the composition compound microbial culture starter.Prepare greenfeed with above-mentioned compound microbial culture starter with following method:
1) raw material is prepared: selected respectively 1 ton of mixture that does not have rotten straw, cornstalk, pulse family class bar, hand hay cutter is long to 2～5cm.
2) actication of culture: the mixed microorganism microbial inoculum 500g that configures more than inciting somebody to action pours in 100 liters of 1 ° of Brix brewer's worts, fully mixes, and 35 ℃ was that activation is good through 2 hours down.
3) inoculation charging: the raw material layer, when accumulation 20cm is thick, spray one time bacterium liquid.Piling up height overall should be above 2 meter.
4) aerobic cultivation: place 10 day in the air summer.
5) anaerobism is cultivated: with the plastic sheeting sealing, and can be edible after 30 days summers for livestock.
The compound microbial culture starter consumption is 0.5% of a raw material.
The greenfeed of fermenting process of preparing, pH are 4.0, and alcohol concentration is 3g/L.Have tart flavour, wine flavour, be yellow green; Content of cellulose reduces, protein content increases.Behind the cattle fooder, the output of milk improves 8%, and sheep calf daily gain improves 8%.
Embodiment 3:
100g aspergillus niger spore, 100g Lactobacillus rhamnosus and the 100g Lactobacillus plantarum of fermentation system are mixed composition, and Lactobacillus rhamnosus is 30.0*10
9Individual/gram, Lactobacillus plantarum quantity is 5*10
9Individual/gram, aspergillus niger spore quantity is 20*10
8Individual/gram.
Prepare greenfeed with above-mentioned mixed bacterium leavening agent with following method:
1) raw material is prepared: selected respectively 1 ton is not had the straw that rots, and hand hay cutter is long to 2～5cm.
2) actication of culture: the mixed microorganism microbial inoculum 700g that configures more than inciting somebody to action pours in 100 liters of 2 ° of Brix brewer's worts, fully mixes, and 25 ℃ was that activation is good through 3 hours down.
3) inoculation charging: the raw material layer, when accumulation 30cm is thick, spray one time bacterium liquid.Piling up height overall should be above 2 meter.
4) aerobic cultivation: place 5 day in the air summer.
5) anaerobism is cultivated: with the plastic sheeting sealing, and can be edible after 20 days summers for livestock.
The compound microbial culture starter consumption is 1% of a raw material.
The greenfeed of fermenting process of preparing, pH are 3.8, and alcohol concentration is 5g/L.Have tart flavour, wine flavour, be yellow green; Content of cellulose reduces, protein content increases.Behind the cattle fooder, the output of milk improves 10%, and sheep calf daily gain improves 10%.
Embodiment 4:
100g aspergillus niger spore, 200g Lactobacillus rhamnosus and the 200g Lactobacillus plantarum of fermentation system are mixed composition, and Lactobacillus rhamnosus is 10.0*10
9Individual/gram, Lactobacillus plantarum quantity is 1*10
9Individual/gram, aspergillus niger spore quantity is 10*10
8Individual/gram.
Prepare greenfeed with above-mentioned mixed bacterium leavening agent with following method:
1) raw material is prepared: selected respectively 1 ton is not had the cornstalk that rots, and hand hay cutter is long to 2～5cm.
2) actication of culture: the mixed microorganism microbial inoculum 400g that configures more than inciting somebody to action pours in 100 liters of 2 ° of Brix brewer's worts, fully mixes, and 25 ℃ was that activation is good through 3 hours down.
3) inoculation charging: the raw material layer, when accumulation 30cm is thick, spray one time bacterium liquid.Piling up height overall should be above 2 meter.
4) aerobic cultivation: place 30 day in the air spring.
5) anaerobism is cultivated: with the plastic sheeting sealing, and can be edible after 90 days springs for livestock.
The compound microbial culture starter consumption is 0.1% of a raw material.
6)
The greenfeed of fermenting process of preparing, pH are 4.0, and alcohol concentration is 1g/L.Have tart flavour, wine flavour, be yellow green; Content of cellulose reduces, protein content increases.Behind the cattle fooder, the output of milk improves 5%, and sheep calf daily gain improves 6%.
Claims (6)
1. method of utilizing the compound microorganism ferments stalk to produce greenfeed, raw material is prepared: straw, cornstalk and pulse family class bar raw material or its mixture that selected nothing is rotted, hand hay cutter is long to 2～5cm; Actication of culture: compound microbial culture starter is poured in 1～2 ° of Brix brewer's wort, mixed, handled 2～3 hours for 25 ℃～35 ℃; Raw water content should be controlled at 70%～80%; The inoculation charging: the raw material layer, when accumulation 20～30cm is thick, spray one time bacterium liquid; Aerobic cultivation: placed in the air 5～30 days; Anaerobism was cultivated: with plastic sheeting sealing 20～90 days; Compound microbial culture starter is made up of aspergillus niger spore, Lactobacillus rhamnosus CGMCC4430 and Lactobacillus plantarum, and the compound microbial culture starter consumption is the 0.1-5% of raw material.
2. as weighing 1 said a kind of method of utilizing the compound microorganism ferments stalk to produce greenfeed; It is characterized in that each bacterial classification viable count weight range is following in the said compound microbial culture starter: Lactobacillus rhamnosus is 109 of (0.1～60.0) */gram; Lactobacillus plantarum quantity is 109 of (0.01～6.0) */gram, and aspergillus niger spore quantity is 108 of (1～50) */gram.
3. like the said a kind of method of utilizing the compound microorganism ferments stalk to produce greenfeed of power 1-2; It is characterized in that Lactobacillus rhamnosus is 109 of (1～60.0) */gram in the said compound microbial culture starter; Lactobacillus plantarum quantity is 109 of (0.1～6.0) */gram, and aspergillus niger spore quantity is 108 of (1～30) */gram.
4. like the said a kind of method of utilizing the compound microorganism ferments stalk to produce greenfeed of power 1-3; It is characterized in that Lactobacillus rhamnosus is a 30.0*109/gram in the said compound microbial culture starter; Lactobacillus plantarum quantity is 5*109/gram, and aspergillus niger spore quantity is 20*108/gram.
5. as weighing 1 said a kind of method of utilizing the compound microorganism ferments stalk to produce greenfeed; It is characterized in that aspergillus niger spore in the said compound microbial culture starter, Lactobacillus rhamnosus powder, Lactobacillus plantarum powder are 1 with mass ratio: (0.5-2): (0.5-2) mix, with dry weight basis.
6. as weighing 1 said a kind of method of utilizing the compound microorganism ferments stalk to produce greenfeed, it is characterized in that said compound microbial culture starter consumption is the 0.1-1% of raw material.
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| CN102870993A (en) * | 2012-10-10 | 2013-01-16 | 荆州双胞胎饲料有限公司 | Compound feed additive for effectively improving intestinal canal health of pigs |
| CN106978378A (en) * | 2017-06-02 | 2017-07-25 | 合肥华盖生物科技有限公司 | A kind of microbial straw leavening and the method using its fermented stalk |
| CN112890023A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Long-straw silage and preparation method thereof |
| CN112890022A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Silage based on microorganisms and preparation method thereof |
| CN114668076A (en) * | 2022-02-07 | 2022-06-28 | 吉林农业大学 | Method for preparing silage by using compound microbial agent |
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| CN101946853A (en) * | 2010-08-25 | 2011-01-19 | 天津农学院 | Method for preparing silage feed by fermenting straw through composite microbes |
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| CN101524180A (en) * | 2009-04-22 | 2009-09-09 | 东北农业大学 | Composite microbiological antibiotic and preparation method thereof |
| CN101810242A (en) * | 2009-11-02 | 2010-08-25 | 韩建春 | Micro-ecological preparation for feed and preparation method thereof |
| CN101946853A (en) * | 2010-08-25 | 2011-01-19 | 天津农学院 | Method for preparing silage feed by fermenting straw through composite microbes |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102870993A (en) * | 2012-10-10 | 2013-01-16 | 荆州双胞胎饲料有限公司 | Compound feed additive for effectively improving intestinal canal health of pigs |
| CN102870993B (en) * | 2012-10-10 | 2013-06-05 | 荆州双胞胎饲料有限公司 | Compound feed additive for effectively improving intestinal canal health of pigs |
| CN106978378A (en) * | 2017-06-02 | 2017-07-25 | 合肥华盖生物科技有限公司 | A kind of microbial straw leavening and the method using its fermented stalk |
| CN106978378B (en) * | 2017-06-02 | 2020-11-10 | 山东健源生物科技有限公司 | Microbial straw leavening agent and method for fermenting straw by using microbial straw leavening agent |
| CN112890023A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Long-straw silage and preparation method thereof |
| CN112890022A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Silage based on microorganisms and preparation method thereof |
| CN114668076A (en) * | 2022-02-07 | 2022-06-28 | 吉林农业大学 | Method for preparing silage by using compound microbial agent |
| CN114668076B (en) * | 2022-02-07 | 2023-11-21 | 吉林农业大学 | Method for preparing yellow storage feed by using composite microbial agent |
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