CN108740312A - A kind of additive for microbe feedstuff and preparation method thereof - Google Patents
A kind of additive for microbe feedstuff and preparation method thereof Download PDFInfo
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- CN108740312A CN108740312A CN201810576653.2A CN201810576653A CN108740312A CN 108740312 A CN108740312 A CN 108740312A CN 201810576653 A CN201810576653 A CN 201810576653A CN 108740312 A CN108740312 A CN 108740312A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
Abstract
The invention discloses a kind of additive for microbe feedstuff, and containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium and lactobacillus plantarum contain per 1kg additive for microbe feedstuff：108.9g S. cervisiae, the living bacteria count amount of S. cervisiae is preferably 5.8 × 107A/g；The living bacteria count amount of the bacillus subtilis of 767.2g, bacillus subtilis is preferably 5.2 × 109The enterococcus faecium and lactobacillus plantarum of a/g, 123.9g, enterococcus faecium and the effective total viable count amount of lactobacillus plantarum are preferably 6.6 × 107A/g, can effectively utilize the production method of 4 kinds of bacterium mixed fermentation production this excellent product of additive for microbe feedstuff well, and additive for microbe feedstuff of the present invention can greatly improve efficiency of feed utilization, significantly reduce aquaculture cost, increase economic efficiency.
Description
Technical field
The present invention relates to technical field of microbial fermentation, specifically a kind of additive for microbe feedstuff and preparation method thereof.
Background technology
Food-safety problem is the significant problem for being related to people's health and national economy, especially China's WTO with
Afterwards, agricultural product security problem has become the key factor for influencing agricultural products in China industrial competitiveness.With social progress and
The improvement of people's living standards, food-safety problem have been to be concerned by more and more people, and Animal husbandry production is to feed addictive
More stringent requirements are proposed for safety.
Meat is the primary animal food of people's intake, and safe feed is the basis of animal food safety.Development is opened
Send out safe to use, the feed additive of advantageous growth of animal is the key that development " non-nuisance technology ", and microorganism fodder
Preparation is the current preferred good method relatively generally acknowledged.With the extensive development of microorganism application technology, in active bacteria formulation
Application aspect, it is desirable to a kind of products can exercise multiple functions, solve the problems, such as various, and many products are all by a variety of
Microorganism group at.Currently, on the production form of this kind of product, it is general using single culture is separated culture, then by a variety of bacterium
Culture mix, form multi-cultur es multifunctional product.This mode of production, it is desirable that each bacterium uses one time fermentation
Tank, it is desirable that investment of production equipment is larger, and energy consumption is higher, substantially increases production cost.At present for having synergistic effect relationship
Bacterial strain screening and combination or a random process, lack effective theoretical direction, and the mixing for having applied is trained
Foster system can not effectively coordinate the relationship between bacterium, it is made to reach best Ecological level, play ceiling effect.This is seriously hindered
The development and application of multi strain co cultivation
Therefore, it is necessary to a kind of additive for microbe feedstuff, improve the growth of animal performance of livestock-raising, reduce cultivation at
This, avoid the feed addictive containing chemical substances such as various hormone quasi-growth promoters, antibiotic be widely applied thus
It avoids feed addictive from for a long time, largely using, makes largely to accumulate a large amount of antibiotic, hormonal substance in body, result in life
The generation of object drug resistance, water body immunity degradation, or even the problem of seriously affecting people's health, occur.
Invention content
The problem of for the above-mentioned feed addictive with the presence of aquatic products, the purpose of the present invention is to provide a kind of feedings of microorganism
Feed additives and preparation method thereof, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides the following technical solutions：
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 4.5 × 10 in 1kg additive for microbe feedstuff7~7.5 × 107The S. cervisiae 84.5- of a/g
140.8g、3.9×109~6.9 × 109The bacillus subtilis 703.3-816.0g of a/g and 5.3 × 107~8.3 × 107
The enterococcus faecium and lactobacillus plantarum 99.5-155.9g of a/g.
As a further solution of the present invention：Contain per 1kg additive for microbe feedstuff：The S. cervisiae of 108.9g is made
The living bacteria count amount of brewer yeast bacterium is preferably 5.8 × 107A/g；The bacillus subtilis of 767.2g, bacillus subtilis
Living bacteria count amount is preferably 5.2 × 109The enterococcus faecium and lactobacillus plantarum of a/g, 123.9g, enterococcus faecium and plant breast
The effective total viable count amount of bacillus is preferably 6.6 × 107A/g.
As a further solution of the present invention：It is 85.4g, dung intestines to contain enterococcus faecium in per 1kg additive for microbe feedstuff
The effective total viable count amount of coccus is preferably 4.55 × 107A/g；Lactobacillus plantarum is 38.4g, and the effective viable bacteria of lactobacillus plantarum is total
Quantity is preferably 2.05 × 107/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 60-100g, husk mixing
30-70g, peptone 50-60g, glucose 4-7g, yeast extract 3-5g, distilled water 1000mL, pH6.8-7.0；Liquid Culture
Condition is：30-34 DEG C of temperature, incubation time 23-28h, shaking speed 180-250r/min；Fermentation culture conditions are：Inoculation
Measure 5-10%, 30-34 DEG C of temperature, incubation time 15-25h；Zymotic fluid and air quantity ratio (m3：m3/ min, similarly hereinafter) it is 1：0.2-
1.2。
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 600-1000g, dregs of beans
350-780g, dextrin 15-23gNa2CO35-40g, Na2HPO410-50g, pH6.5-7.5；Liquid culture condi is：Temperature
28-40 DEG C, incubation time 43-48h, shaking speed 180-250r/min of degree；Fermentation culture conditions are：Inoculum concentration 5-
10%, 28-40 DEG C of temperature, incubation time 25-35h；Zymotic fluid and air quantity ratio (m3：m3/ min, similarly hereinafter) it is 1：0.2-1.2.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 10-20g, tryptone 20-
40g, beef extract 3-7g, yeast extract 1-5g, dipotassium hydrogen phosphate 1-3g, agar 20-25g, pH6.0-6.5；Liquid is trained
Foster condition is：32-37 DEG C of temperature, incubation time 16-28h, shaking speed 120-190r/min；Fermentation culture conditions are：It connects
Kind amount 5-10%, 32-37 DEG C of temperature, incubation time 13-18h.Zymotic fluid is 1 with air quantity ratio：0.2-1.2.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention is proposed carries out mixing training by S. cervisiae, bacillus subtilis, enterococcus faecium and lactobacillus plantarum
It supports, creatively proposes fermentation technique, and had investigated well simultaneously for four kinds of bacterium mixed fermentation of the present invention
Culture medium, four kinds of bacterium mixing fermentation culture conditions, these are all that no pertinent literature is reported.And product of the present invention can succeed
The crux of preparation lies also in the determination of above-mentioned condition.
Feeding of the product of the present invention for animals such as pig and chickens has growth promotion, raising feedstuff-meat ratio, raising immune animal
Power, reduce environmental pollution and prevent disease occur etc. functions；The vigor of animal sperm can be improved, rate of teratosperm is reduced；Meat
Hormone and antibiotic residue are not present in product, avoids drug resistance and medicament residue, me can be improved in popularization and application of the invention
The competitiveness in the international market of state's meat products, it is ensured that the quality of meat products and safe and healthy, is new one after antibiotic
For product.Thus the present invention be one kind can effectively utilize 4 kinds of bacterium well mixed fermentation production additive for microbe feedstuff this
The production method of kind excellent product, the present invention can substantially reduce production cost, promote the popularization and application of such product, the present invention micro-
Biological feed additive can greatly improve efficiency of feed utilization, significantly reduce aquaculture cost, increase economic efficiency.
Culture presevation mode of the present invention
S. cervisiae (Saccharomycescerevisiae) is purchased from American Type Culture Collection center, bacterial strain preservation
Number is ATCC9763；
Bacillus subtilis strain (Bacillussubtilis) is purchased from Chinese industrial Microbiological Culture Collection administrative center,
Bacterial strain deposit number is CICC20872；
Enterococcus faecium (Enterococcusfaecium) is purchased from China Committee for Culture Collection of Microorganisms's commonly micro- life
Object center, culture presevation number：CGMCCNo.8296, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese science
Institute of microbiology of institute.
Lactobacillus plantarum (Lactobacillusplantarum) is general purchased from China Committee for Culture Collection of Microorganisms
Logical microorganism center, bacterial strain preserving number：CGMCCNo.6312, preservation address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Institute of Microorganism, Academia Sinica.
Specific implementation mode
The technical solution of this patent is described in more detail With reference to embodiment.
Following fluid nutrient mediums are prepared on the basis of 1L, and fermentation medium is prepared on the basis of 2.5t.
Embodiment 1
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 4.5 × 10 in 1kg additive for microbe feedstuff7The S. cervisiae 84.5g of a/g, 3.9 × 109
The bacillus subtilis 816.0g of a/g and 5.3 × 107The enterococcus faecium and lactobacillus plantarum 99.5g of a/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 60g, husk mixing 35g, egg
White peptone 50g, glucose 4g, yeast extract 3g, distilled water 1000mL, pH6.8；Liquid culture condi is：31 DEG C of temperature, incubation time
28h, shaking speed 190r/min；Fermentation culture conditions are：Inoculum concentration 6%, 31 DEG C of temperature, incubation time 18h；Zymotic fluid and wind
Amount is than (m3：m3/ min, similarly hereinafter) it is 1：0.6.
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 650g, dregs of beans 380g, dextrin
17gNa2CO335g, Na2HPO415g, pH6.8；Liquid culture condi is：39 DEG C, incubation time 445h of temperature, shaking speed
190r/min；Fermentation culture conditions are：Inoculum concentration 7%, 39 DEG C of temperature, incubation time 25h；Zymotic fluid and air quantity ratio (m3：m3/
Min, similarly hereinafter) it is 1：0.6.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 10g, tryptone 25g, beef
Cream 7g, yeast extract 4g, dipotassium hydrogen phosphate 2g, agar 23g, pH6.3；Liquid culture condi is：35 DEG C of temperature, incubation time
18h, shaking speed 170r/min；Fermentation culture conditions are：Inoculum concentration 9%, 35 DEG C of temperature, incubation time 13h.Zymotic fluid and wind
Amount is than being 1：0.2.
Embodiment 2
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 4.9 × 10 in 1kg additive for microbe feedstuff7The S. cervisiae 94.8g of a/g, 4.7 × 109
The bacillus subtilis 773.6g of a/g and 6.9 × 107The enterococcus faecium and lactobacillus plantarum 131.6g of a/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 80g, husk mixing 60g, egg
White peptone 55g, glucose 6g, yeast extract 4g, distilled water 1000mL, pH7.0；Liquid culture condi is：33 DEG C of temperature, incubation time
26h, shaking speed 240r/min；Fermentation culture conditions are：Inoculum concentration 9%, 33 DEG C of temperature, incubation time 17h；Zymotic fluid and wind
Amount is than (m3：m3/ min, similarly hereinafter) it is 1：0.8.
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 760g, dregs of beans 480g, dextrin
16gNa2CO333g, Na2HPO442g, pH7.1；Liquid culture condi is：35 DEG C, incubation time 46h of temperature, shaking speed
230r/min；Fermentation culture conditions are：Inoculum concentration 6%, 35 DEG C of temperature, incubation time 29h；Zymotic fluid and air quantity ratio (m3：m3/
Min, similarly hereinafter) it is 1：0.6.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 16g, tryptone 23g, beef
Cream 4g, yeast extract 5g, dipotassium hydrogen phosphate 2g, agar 23g, pH6.5；Liquid culture condi is：36 DEG C of temperature, incubation time
27h, shaking speed 180r/min；Fermentation culture conditions are：Inoculum concentration 6%, 36 DEG C of temperature, incubation time 15h.Zymotic fluid and wind
Amount is than being 1：0.8.
Embodiment 3
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 6.7 × 10 in 1kg additive for microbe feedstuff7The S. cervisiae 105.9g of a/g, 5.6 × 109
The bacillus subtilis 777.8g of a/g and 6.8 × 107The enterococcus faecium and lactobacillus plantarum 116.3g of a/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 89g, husk mixing 61g, egg
White peptone 56g, glucose 6g, yeast extract 4g, distilled water 1000mL, pH6.9；Liquid culture condi is：34 DEG C of temperature, incubation time
25h, shaking speed 240r/min；Fermentation culture conditions are：Inoculum concentration 9%, 34 DEG C of temperature, incubation time 25h；Zymotic fluid and wind
Amount is than (m3：m3/ min, similarly hereinafter) it is 1：0.6.
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 850g, dregs of beans 530g, dextrin
20gNa2CO326g, Na2HPO445g, pH6.7；Liquid culture condi is：36 DEG C, incubation time 44h of temperature, shaking speed
220r/min；Fermentation culture conditions are：Inoculum concentration 8%, 36 DEG C of temperature, incubation time 31h；Zymotic fluid and air quantity ratio (m3：m3/
Min, similarly hereinafter) it is 1：0.7.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 15g, tryptone 35g, beef
Cream 5g, yeast extract 5g, dipotassium hydrogen phosphate 2g, agar 23g, pH6.2；Liquid culture condi is：35 DEG C of temperature, incubation time
25h, shaking speed 150r/min；Fermentation culture conditions are：Inoculum concentration 7%, 35 DEG C of temperature, incubation time 16h.Zymotic fluid and wind
Amount is than being 1：0.7.
Embodiment 4
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 5.8 × 10 in 1kg additive for microbe feedstuff7The S. cervisiae 96.4g of a/g, 6.7 × 109
The bacillus subtilis 765.4g of a/g and 6.2 × 107The enterococcus faecium and lactobacillus plantarum 138.2g of a/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 85g, husk mixing 50g, egg
White peptone 55g, glucose 6g, yeast extract 5g, distilled water 1000mL, pH7.0；Liquid culture condi is：32 DEG C of temperature, incubation time
23h, shaking speed 250r/min；Fermentation culture conditions are：Inoculum concentration 9%, 32 DEG C of temperature, incubation time 18h；Zymotic fluid and wind
Amount is than (m3：m3/ min, similarly hereinafter) it is 1：0.7.
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 850g, dregs of beans 680g, dextrin
19gNa2CO325g, Na2HPO435g, pH6.9；Liquid culture condi is：36 DEG C, incubation time 46h of temperature, shaking speed
220r/min；Fermentation culture conditions are：Inoculum concentration 6%, 36 DEG C of temperature, incubation time 25h；Zymotic fluid and air quantity ratio (m3：m3/
Min, similarly hereinafter) it is 1：0.8.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 15g, tryptone 23g, beef
Cream 4g, yeast extract 3g, dipotassium hydrogen phosphate, 2g, agar 23g, pH6.3；Liquid culture condi is：34 DEG C of temperature, when culture
Between 18h, shaking speed 170r/min；Fermentation culture conditions are：Inoculum concentration 5%, 34 DEG C of temperature, incubation time 17h.Zymotic fluid with
Air quantity ratio is 1：0.9.
Embodiment 5
A kind of additive for microbe feedstuff, containing there are four types of strain, S. cervisiae, bacillus subtilis, enterococcus faecium
And lactobacillus plantarum, often contain 5.8 × 10 in 1kg additive for microbe feedstuff7The S. cervisiae 108.9g of a/g, 5.2 × 109
The bacillus subtilis 767.2g of a/g and 6.6 × 107The enterococcus faecium and lactobacillus plantarum 123.9g of a/g.
The production method of mentioned microorganism feed addictive comprising following steps：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermentation
It is spray-dried, is crushed after culture, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；
It is spray-dried, is crushed after fermented and cultured, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately subjected to liquid
Then body culture carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
Specifically,
Liquid Culture and fermented and cultured used medium group become in step 1)：Oils cake powder 85g, husk mixing 46g, egg
White peptone 54g, glucose 5g, yeast extract 5g, distilled water 1000mL, pH6.9；Liquid culture condi is：34 DEG C of temperature, incubation time
26h, shaking speed 230r/min；Fermentation culture conditions are：Inoculum concentration 7%, 34 DEG C of temperature, incubation time 22h；Zymotic fluid and wind
Amount is than (m3：m3/ min, similarly hereinafter) it is 1：0.8.
Liquid Culture and fermented and cultured used medium group become in step 2)：Cornstarch 800g, dregs of beans 650g, dextrin
18gNa2CO335g, Na2HPO440g, pH7.1；Liquid culture condi is：29 DEG C, incubation time 44h of temperature, shaking speed
210r/min；Fermentation culture conditions are：Inoculum concentration 6%, 29 DEG C of temperature, incubation time 28h；Zymotic fluid and air quantity ratio (m3：m3/
Min, similarly hereinafter) it is 1：0.9.
Liquid Culture and fermented and cultured used medium group become in step 3)：Glucose 15g, tryptone 35g, beef
Cream 5g, yeast extract 3g, dipotassium hydrogen phosphate 2g, agar 23g, pH6.4；Liquid culture condi is：36 DEG C of temperature, incubation time
23h, shaking speed 160r/min；Fermentation culture conditions are：Inoculum concentration 7%, 36 DEG C of temperature, incubation time 15h.Zymotic fluid and wind
Amount is than being 1：0.9.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, one skilled in the relevant art within the scope of knowledge, can also be under the premise of not departing from this patent objective
It makes a variety of changes.
Claims (7)
1. a kind of additive for microbe feedstuff, which is characterized in that it contains there are four types of strain, S. cervisiae, bacillus subtilis,
Enterococcus faecium and lactobacillus plantarum often contain 4.5 × 10 in 1kg additive for microbe feedstuff7~7.5 × 107The saccharomyces cerevisiae of a/g
Bacterium 84.5-140.8g, 3.9 × 109~6.9 × 109The bacillus subtilis 703.3-816.0g of a/g and 5.3 × 107~
8.3×107The enterococcus faecium and lactobacillus plantarum 99.5-155.9g of a/g.
2. additive for microbe feedstuff according to claim 1, which is characterized in that contain per 1kg additive for microbe feedstuff：
The living bacteria count amount of the S. cervisiae of 108.9g, S. cervisiae is preferably 5.8 × 107A/g；767.2g withered grass bud
The living bacteria count amount of spore bacillus, bacillus subtilis is preferably 5.2 × 109The enterococcus faecium of a/g, 123.9g and plant breast
Bacillus, enterococcus faecium and the effective total viable count amount of lactobacillus plantarum are preferably 6.6 × 107A/g.
3. additive for microbe feedstuff according to claim 2, which is characterized in that per in 1kg additive for microbe feedstuff
It is 85.4g containing enterococcus faecium, the effective total viable count amount of enterococcus faecium is preferably 4.55 × 107A/g；Lactobacillus plantarum is
38.4g, the effective total viable count amount of lactobacillus plantarum is preferably 2.05 × 107/g.
4. a kind of preparation method of additive for microbe feedstuff as described in any one of claims 1-3, which is characterized in that including such as
Lower step：
1) S. cervisiae is produced：By the advanced row Liquid Culture of saccharomyces cerevisiae bacteria strain, fermented and cultured is then carried out；Fermented and cultured
After be spray-dried, crush, it is spare；
2) bacillus subtilis is produced：Bacillus subtilis strain is first subjected to Liquid Culture, then carries out fermented and cultured；Fermentation
It is spray-dried, is crushed after culture, it is spare；
3) enterococcus faecium and lactobacillus plantarum are produced：Manure enterococcin strain and lactobacillus plantarum strain are first separately carried out to liquid training
It supports, then carries out fermented and cultured；It is spray-dried, is crushed respectively after fermented and cultured, mixing is spare；
4) by step 1), 2) and 3) obtained solid powder mix in proportion to get.
5. the preparation method of additive for microbe feedstuff according to claim 4, which is characterized in that liquid is trained in step 1)
It supports and fermented and cultured used medium group becomes：Oils cake powder 60-100g, husk mixing 30-70g, peptone 50-60g,
Glucose 4-7g, yeast extract 3-5g, distilled water 1000mL, pH6.8-7.0；Liquid culture condi is：30-34 DEG C of temperature, training
Support time 23-28h, shaking speed 180-250r/min；Fermentation culture conditions are：Inoculum concentration 5-10%, temperature 30-34
DEG C, incubation time 15-25h；Zymotic fluid and air quantity ratio (m3：m3/ min, similarly hereinafter) it is 1：0.2-1.2.
6. the preparation method of additive for microbe feedstuff according to claim 4, which is characterized in that liquid is trained in step 2)
It supports and fermented and cultured used medium group becomes：Cornstarch 600-1000g, dregs of beans 350-780g, dextrin 15-
23gNa2CO35-40g, Na2HPO410-50g, pH6.5-7.5；Liquid culture condi is：28-40 DEG C of temperature, incubation time
43-48h, shaking speed 180-250r/min；Fermentation culture conditions are：Inoculum concentration 5-10%, 28-40 DEG C of temperature, culture
Time 25-35h；Zymotic fluid and air quantity ratio (m3：m3/ min, similarly hereinafter) it is 1：0.2-1.2.
7. the preparation method of additive for microbe feedstuff according to claim 4, which is characterized in that liquid is trained in step 3)
It supports and fermented and cultured used medium group becomes：Glucose 10-20g, tryptone 20-40g, beef extract 3-7g, yeast carry
Take object 1-5g, dipotassium hydrogen phosphate 1-3g, agar 20-25g, pH6.0-6.5；Liquid culture condi is：32-37 DEG C of temperature,
Incubation time 16-28h, shaking speed 120-190r/min；Fermentation culture conditions are：Inoculum concentration 5-10%, temperature 32-37
DEG C, incubation time 13-18h.Zymotic fluid is 1 with air quantity ratio：0.2-1.2.
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| CN109527245A (en) * | 2018-12-27 | 2019-03-29 | 北京市畜牧总站 | The preparation method of one boar composite probiotics preparations |
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| CN112522142A (en) * | 2020-12-04 | 2021-03-19 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Licorice residue feed fermentation inoculant and application thereof |
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| CN109527245A (en) * | 2018-12-27 | 2019-03-29 | 北京市畜牧总站 | The preparation method of one boar composite probiotics preparations |
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| CN112522142A (en) * | 2020-12-04 | 2021-03-19 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Licorice residue feed fermentation inoculant and application thereof |
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| CN114304379A (en) * | 2022-01-10 | 2022-04-12 | 南京工业大学 | Preparation method of fermented feed containing compound microbial agent |
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