CN102008012B - Production method of fungal fermentation feed - Google Patents
Production method of fungal fermentation feed Download PDFInfo
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- CN102008012B CN102008012B CN2010105542910A CN201010554291A CN102008012B CN 102008012 B CN102008012 B CN 102008012B CN 2010105542910 A CN2010105542910 A CN 2010105542910A CN 201010554291 A CN201010554291 A CN 201010554291A CN 102008012 B CN102008012 B CN 102008012B
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Abstract
The invention discloses a production method of a fungal fermentation feed. The method comprises slant strain activation culture, liquid culture, liquid secondary seed culture and solid fermentation culture. The method provided by the invention is characterized in that the Schizophyllum commune is cultured at the temperature of 20-27 DEG C in the solid fermentation phase; after the Schizophyllum commune is cultured for 5-8 days, the temperature is reduced to 6-10 DEG C; after the Schizophyllum commune is cultured for 1-3 days, the temperature is increased to 20-27 DEG C; and the total culture time is 15-40 days. The product obtained by the method has stable performance, can be used safely, and can be matched with other feed without taboos. The additive amount of the product provided by the invention in the feed is 20-100%.After feeding, the immunity and milking capacity of the animal organism can be obviously enhanced, and the daily gain and the feed conversion rate can be improved. The method provided by the invention meets the green production requirements of animal foods and has the advantages of obvious social benefit and economic benefit.
Description
Technical field
Belong to microorganism field, particularly fungus feed product manufacture field
Background technology:
Schizophyllum commune (Schizophyllum commune (Fr.) Fr.) has another name called white ginseng, tree flower, smallpox bacterium etc., Schizophyllum Eumycota, Basidiomycotina, Hymenomycetes, Aphyllophorales, schizophyllum commune section, Schizophyllum.Schizophyllum commune is the traditional edible and medicinal fungi of a kind of China, contains the active ingredients such as Schizophyllum commune Fr polysaccharides, nucleosides, adenosine, purine.In the schizophyllum commune born of the same parents and the outer polysaccharide of born of the same parents can promote the part of macrophage to move and strengthen its activity, also can inducing T cell, the cytohormone of NK cell and many and Immune interrelation produces and complement activation, has strong antitumor activity.Schizophyllum commune is flat, flavor is sweet, can clearing liver, make eye bright, lactagogue, nourishing and fit keeping function, and can treat women's leukorrhea, lassitude, dizzy tinnitus, abnormal sweating.Schizophyllum commune is mainly cultivated the dual mode acquisition by artificial cultivation and submerged fermentation at present, and it is short that the schizophyllum commune fermented and cultured has the cycle, the characteristics that output is large.
At present, the direction of domestic research to schizophyllum commune has: Schizophyllum commune Fr polysaccharides extracts to be identified and the research of polysaccharide pharmacological effect, the research of submerged fermentation condition, and the research of schizophyllum commune artificial cultivation method utilizes the research of schizophyllum commune production malic acid.The schizophyllum commune product is less report, develops as early as possible schizophyllum commune class Related product, is a good thing that promotes the well-being of mankind.
Solid fermentation is the effective ways that obtain schizophyllum commune.Resulting mycoplasma contains the metabolite of thalline behind the solid fermentation, and mycoplasma itself also is good nutriment.The mycoplasma that solid fermentation obtains and thalline are to have nutrition and medicinal function, are having a good application prospect aspect the Application and Development of natural feed addictive.
Feed additive industry is along with China cultivation industry flourish also obtained development at full speed, in order to improve the growth of animal performance, reduce aquaculture cost, comprise antibiotic, the feed addictives such as steroids growth promoter (Clenbuterol and testosterone etc.) once or continue to be used widely, they have made huge contribution to the development of animal husbandry.But the negative effect of the use that this type of feed addictive is long-term, a large amount of also comes out day by day significantly.Be in particular in: the generation of livestock and poultry and human body drug resistance, the materials such as antibiotic form residual at body, and animal immunizing power descends, and human body produces many bad reactions etc., and the calendar year 2001 outlet that meets with of the Livestock Products in China main cause of being obstructed is exactly antibiotic residual.
At present, the internal feed additives industries is in a transitional period, low-residual or noresidue, without the resistance to the action of a drug, natural green feed additive will replace gradually antibiotic and other has the feed addictive of residual contamination problem.Green feed additive, must possess " can strengthen animal immunity, can obviously improve the conversion ratio of feed and quantum of output and the environmentally safe of animal " etc. basic demand.The use of green feed additive is the basis that ensures animal food safety.It meets the developing direction of feed addictive, and green feed additive will become the main product in feed addictive market, and the use of green feed additive is the primary condition that ensures China's meat products safety.
At present, the green feed additive of China is to some extent development also, and active bacteria formulation, saccharicter-penin, compound sugar, fodder enzyme preparation, Chinese herbal feed additive are main several.There are the problems such as product standard lacks, technical merit is on the low side, the product function composition is indefinite, addition is large in green feed additive at present.Stable and controllable for quality, noresidue, non-environmental-pollution, use safety, not limited by raw material, the green feed additive product that properties of product are stable is anxious to be waited to develop.
Summary of the invention:
The technical problem that the present invention solves has provided a kind of production method of fungal fermentation feed.
Product of the present invention is comprised of thalline and the mycoplasma that the schizophyllum commune solid fermentation obtains;
Described schizophyllum commune solid fermentation stage cultivation temperature is 20-27 ℃, cultivates after 5-8 days, reduces the temperature to 6-10 ℃, cultivates 1-3 days, improves subsequently temperature to 20-27 ℃, total incubation time 15-40 days.
Product processes step of the present invention comprises the steps: seed culture: shaking table is cultivated the schizophyllum commune liquid seeds; Solid fermentation: switching schizophyllum commune liquid seeds solid fermentation culture medium fermented and cultured; Drying and crushing: ferment complete drying solid fermentation medium on fluid bed or other drying equipments, dry complete the pulverizing; Packing: the direct packing of comminuting matter both can.
Production method of fungal fermentation feed is as follows:
(1) slant strains activation culture: the slant strains of preservation is transferred on the slant medium, cultivated about 96～120 hours, and covered with the inclined-plane to mycelia and get final product for 22～28 ℃; 25 ℃ of optimum culturing temperatures.
(2) the liquid first order seed is cultivated: access of above-mentioned slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate, condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; 150 rev/mins of rotary shaking tables are advisable, and cultivation temperature is advisable with 25 ℃.
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5～20% inoculum concentrations accesses carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate, condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; Suitable inoculum concentration is 10%, and 150 rev/mins of rotary shaking tables are advisable, and cultivation temperature is advisable with 25 ℃.
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 5～10% inoculum concentrations, condition of culture: charge 50%, cultivation temperature 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, humidity 65-80%, ventilation 0.1-0.5 (V/V), incubation time 1-3 days.Improve subsequently temperature to 20-27 ℃, humidity 75-90%, ventilation 0.3-1.0 (V/V), total incubation time 15-40 days.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and selects the multiple drying means such as fluid bed, and dry materials is arrived moisture below 7%.The solid fermentation material is pulverized.
(6) packing.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The carbon source of solid fermentation culture medium of the present invention is mainly selected the raw materials commonly used such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can be selected groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts that need to add have potassium dihydrogen phosphate, magnesium sulfate.
The liquid fermentation seed culture medium forms among the present invention: starch 2～3%, sucrose 3～5%, glucose 1～3%, peptone 0.5%, De-fatted soya protein Powder 2～3%, potassium dihydrogen phosphate 0.13～0.2%, magnesium sulfate 0.1～0.15%, Cobastab
12PPm, PH6～7.
The schizophyllum commune cultural method is fit to large scale fermentation cultivation manufacturing feed among the present invention.
Studies show that by experiment: the schizophyllum commune feed product has good lactagogue effect,
Safety is used in the stable performance of product of the present invention, with other feed all without incompatibility.The addition of product of the present invention in feed is 20-100%, feeding after, can significantly strengthen immunity and the milking capacity of animal body, improve daily gain and feed conversion rate.According to test, the animal milk water secretory volume of feeding feed of the present invention significantly improves, and sees Table 1, meets the greenization production requirement of animal food, and Social benefit and economic benefit is very remarkable.
Product schizophyllum commune feed product of the present invention is take Chinese traditional edible and medical fungi of biological fermentation process production as primary raw material, constant product quality is controlled, in vivo noresidue of product, non-environmental-pollution, without the resistance to the action of a drug, and can improve immunity, can improve culture benefit.Product meets the requirement of green feed or feed addictive fully.
Protein content 10-16% in the product of the present invention, polyoses content reaches 8-12%, contains simultaneously vitamin E, B1, B2.Lysine, each seed amino acid that the human bodies such as glutamic acid are necessary and various trace element.Product has abundant nutritive value.
Because schizophyllum commune has higher nutritive value and bacteria resistance function, can strengthen the animal lactation ability, and the noresidue environmentally safe.Produce schizophyllum commune and it is processed as feed by biofermentation technique, the developing direction that meets current feed fully, it is a kind of green feed, the special lactagogue function of product that adopted biofermentation low temperature stimulation technology Effective Raise, product has stable and controllable for quality, noresidue, non-environmental-pollution, use safety, not limited by raw material, and properties of product are stable, with low cost, be convenient to the schizophyllum commune feed in the promotion and application of feedstuff industry.
Milking capacity effect effect experiment.
Respectively two groups of milk cows are fed experiment, fed review time section milk crop 40 days.
Each time period milk crop experimental unit: kg of table 1 raising dairy cattle
The specific embodiment:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1 (schizophyllum commune feed product production method)
A kind of schizophyllum commune feed product mainly is comprised of the Schizophyllum commune for Mycelium after pulverizing and the dry solid content in the schizophyllary fermentating liquid, this routine product exists with powder-form, formed by schizophyllum commune solid fermentation thalline and mycoplasma in the product, the production process of product of the present invention is as follows: the schizophyllum commune solid fermentation is cultivated, the schizophyllum commune solid fermentation culture is pulverized dry, and packing is product.
Embodiment 2
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 22 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 150 rev/mins of rotary shaking tables, cultivated about 130 hours for 25 ℃;
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5% inoculum concentration access carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 100 hours for 25 ℃;
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 10% inoculum concentration, condition of culture: charge 50%, 24 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 6 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 3 days.Improve subsequently temperature to 26 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 26 days.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and the solid fermentation material is selected the multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying is complete is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 70% wheat bran, 1.4% sucrose, 10% corn flour, 16% corncob (after pulverizing), 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.
The liquid fermentation seed culture medium forms among the present invention: starch 2%, glucose 3%, peptone 0.3%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, Cobastab
12PPm, PH6.Schizophyllum commune (Schizophyllumcommune Fr.) bacterial classification cfcc 82418 is purchased from China Forest microorganism fungus kind preservation administrative center.
Embodiment 3
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 28 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5% inoculum concentration access carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 10% inoculum concentration, condition of culture: charge 50%, 22 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 7 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 1 day.Improve subsequently temperature to 25 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 35 days.Opened every 3-10 days in case of necessity and stirred 10 minutes.
(5) fermented and cultured finishes, and the solid fermentation material is selected the multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying is complete is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 80% wheat bran, 1.4% sucrose, 8% corn flour, 8% corncob (after pulverizing), 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.
The liquid fermentation seed forms among the present invention: starch 2%, glucose 3%, peptone 0.3%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, Cobastab
12PPm, pH6.
Bacterial classification: schizophyllum commune CPCC 810155 (Schizophyllum commune) is purchased from Chinese medicinal microorganism fungus kind preservation administrative center.
Claims (1)
1. a production method of fungal fermentation feed is characterized in that, production process is as follows:
(1) slant strains activation culture: with the schizophyllum commune CPCC 810155 of preservation (
Schizophyllum commune) slant strains is transferred on the slant medium, cultivated 96 hours, and covered with the inclined-plane to mycelia and get final product for 28 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in the 500ml shaking flask of 100ml culture medium and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated 96 hours for 28 ℃;
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5% inoculum concentration access carries out secondary seed in the 500ml shaking flask of 100ml culture medium and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated 96 hours for 28 ℃;
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 10% inoculum concentration, condition of culture: charge 50%, 22 ℃ of cultivation temperature are cultivated after 8 days, reduce the temperature to 7 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 1 day, improve subsequently temperature to 25 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 35 days;
Consisting of of described solid fermentation culture medium: corncob, 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast after 80% wheat bran, 1.4% sucrose, 8% corn flour, 8% are pulverized, pH nature;
(5) fermented and cultured finishes, and uses fluid bed to carry out drying to the solid fermentation material, and baking temperature is controlled at below 80 ℃, and dry materials is arrived moisture below 7%, and the material that drying is complete is pulverized;
(6) packing of product.
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| CN102366028B (en) * | 2011-10-11 | 2012-09-26 | 北京东方联鸣科技发展有限公司 | Efficient nutrient supplementation feed for dairy cattle and application of efficient nutrient supplementation feed |
| CN102406066B (en) * | 2011-10-11 | 2013-01-16 | 中北大学 | Feed additive for dairy cow and preparation method thereof |
| CN103156073B (en) * | 2011-12-13 | 2015-02-18 | 北京东方联鸣科技发展有限公司 | Nutritional supplement for postpartum dairy cows |
| CN102511677B (en) * | 2011-12-15 | 2013-07-31 | 北京锐晟明杰科技发展有限公司 | Feed additive for cow |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254236A (en) * | 2008-04-14 | 2008-09-03 | 南京中医药大学 | Solid body fermentation method taking medicinal fungus for implementing toxicity-reducing and depositing of poison nut |
| CN101643759A (en) * | 2009-07-31 | 2010-02-10 | 中国农业科学院农产品加工研究所 | Method for preparing Schizophyllum commune Fr polysaccharides and dedicated culture medium thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254236A (en) * | 2008-04-14 | 2008-09-03 | 南京中医药大学 | Solid body fermentation method taking medicinal fungus for implementing toxicity-reducing and depositing of poison nut |
| CN101643759A (en) * | 2009-07-31 | 2010-02-10 | 中国农业科学院农产品加工研究所 | Method for preparing Schizophyllum commune Fr polysaccharides and dedicated culture medium thereof |
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