CN105639142A - Production method of pig feed additive - Google Patents
Production method of pig feed additive Download PDFInfo
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- CN105639142A CN105639142A CN201610007393.8A CN201610007393A CN105639142A CN 105639142 A CN105639142 A CN 105639142A CN 201610007393 A CN201610007393 A CN 201610007393A CN 105639142 A CN105639142 A CN 105639142A
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- gram
- feed
- feed additive
- sterilizing
- seed liquor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of feed additives and particularly relates to a production method of a pig feed additive. The production method comprises the following steps: uniformly mixing corn flour and bran, and then adding water and uniformly blending; sterilizing and cooling, then inoculating aspergillus oryzae and rhodotorula glutinis in a manual climatic box and culturing for 5-6 days; after culturing, drying fermented materials in the sun and crushing the fermented materials to obtain fermented feed powder; adding feed-grade lipase into the fermented feed powder; and uniformly stirring to obtain a finished product. The feed additive contains carotenoids, ergosterol, unsaturated fatty acids, protein polypeptides, a plurality of types of vitamins and a plurality of types of digestive enzymes, so that after pigs eat the feed additive, the growth and calcium supplementation can be accelerated, the immunity can be enhanced, diseases can be reduced, and the dosage of antibiotics can be reduced.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to the manufacture method of a kind of pig's feed additive.
Background technology
After antibiotic is widely used in feedstuff as feed additive, it all plays a significant role in preventing and treating Animal diseases, guarantee animal health, promoting growth of animal and production, raising efficiency of feed utilization etc. But while antibiotic promotes animal husbandry development, bringing harm also to all many-sides such as human health and food safety, in such as livestock products, antibiotic residual directly threatens the health of people, also can cause Drug resistance and cause ecological pollution simultaneously. Now, the urgent back to nature of the mankind, it is necessary to safety food, thus develop antibiotic succedaneum, exploitation nonreactive cultural technique has just become the study hotspot of animal husbandry researcher.
Summary of the invention
It is an object of the invention to provide the manufacture method of a kind of pig's feed additive, the pig's feed additive made by the method for the present invention has enhancing immunity, promote grow, the generation of disease of replenishing the calcium, reduce, reduce antibiotic consumption etc. and act on.
The object of the present invention is achieved like this:
Weigh sucrose 3 grams, yeast extract 1 gram, sodium nitrate 0.2 gram, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 28��30 DEG C, inoculate aspergillus oryzae, 28��30 DEG C, rotary shaker 100��140rpm when cultivate within 40��48 hours, namely obtain aspergillus oryzae seed liquor.
Weigh glucose 5 grams, peptone 1 gram, 0.4 gram of ammonium sulfate, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 25��28 DEG C, inoculate rhodotorula glutinis, 25��28 DEG C, rotary shaker 160��200rpm when cultivate within 18��22 hours, namely obtain rhodotorula glutinis seed liquor.
Semen Maydis flour and wheat bran is weighed respectively according to the ratio of mass ratio 1:3, add water after mixing, make water content control for 65��68%, when 0.15MPa after sterilizing 30 minutes, it is cooled to 27��29 DEG C, access the aspergillus oryzae seed liquor of 5% and the rhodotorula glutinis seed liquor of 5%, mix thoroughly, tiling is to tray, thickness 5��8 centimetres, cover sterile gauze above, put in growth cabinet and cultivate, cultivation temperature 27��29 DEG C, growth cabinet relative humidity 90��95%, incubation time 5��6 days, it is placed under sunlight after fermentation ends to be exposed to the sun fermentation material to moisture less than 10%, fermented feed powder is obtained after pulverizing with pulverizer again, feed grade lipase is added according to the ratio of 3��4 units of every gram of fermented feed powder, stir and get product.
The feed additive of the present invention, containing carotenoid, ergosterol, unsaturated fatty acid, protein polypeptide, multivitamin and multiple digestive enzymes, after pig feed, it is possible not only to promote growth and replenish the calcium, can also enhancing immunity, reduce disease generation, reduce antibiotic consumption. When feeding pigs, the feed additive of the present invention is joined mix homogeneously in mixed feed in the ratio of 2��3%.
Specific embodiments
Strain Aspergillus oryzae CICC2101 and rhodotorula glutinis CICC32717 that the present invention adopts are purchased from Chinese industrial Microbiological Culture Collection administrative center.
Weigh sucrose 3 grams, yeast extract 1 gram, sodium nitrate 0.2 gram, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 29 DEG C, inoculate aspergillus oryzae, 29 DEG C, rotary shaker 120rpm when cultivate within 44 hours, namely obtain aspergillus oryzae seed liquor.
Weigh glucose 5 grams, peptone 1 gram, 0.4 gram of ammonium sulfate, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 27 DEG C, inoculate rhodotorula glutinis, 27 DEG C, rotary shaker 180rpm when cultivate within 20 hours, namely obtain rhodotorula glutinis seed liquor.
Semen Maydis flour and wheat bran is weighed respectively according to the ratio of mass ratio 1:3, add water after mixing, make water content control for 67%, when 0.15MPa after sterilizing 30 minutes, it is cooled to 28 DEG C, access the aspergillus oryzae seed liquor of 5% and the rhodotorula glutinis seed liquor of 5%, mix thoroughly, tiling is to tray, thickness 6 centimetres, cover sterile gauze above, put in growth cabinet and cultivate, cultivation temperature 28 DEG C, growth cabinet relative humidity 90%, incubation time 5 days, it is placed under sunlight after fermentation ends to be exposed to the sun fermentation material to moisture less than 10%, fermented feed powder is obtained after pulverizing with pulverizer again, feed grade lipase is added according to the ratio of 4 units of every gram of fermented feed powder, stir and get product.
Claims (1)
1. a manufacture method for pig's feed additive, is characterized in that being prepared from through the following course of processing:
(1) weigh sucrose 3 grams, yeast extract 1 gram, sodium nitrate 0.2 gram, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 28��30 DEG C, inoculate aspergillus oryzae, 28��30 DEG C, rotary shaker 100��140rpm when cultivate within 40��48 hours, namely obtain aspergillus oryzae seed liquor;
(2) weigh glucose 5 grams, peptone 1 gram, 0.4 gram of ammonium sulfate, potassium dihydrogen phosphate 0.1 gram, 0.05 gram of magnesium sulfate, add 100 milliliters of clear water stirring and dissolving, sterilizing 20 minutes at 121 DEG C, be cooled to 25��28 DEG C, inoculate rhodotorula glutinis, 25��28 DEG C, rotary shaker 160��200rpm when cultivate within 18��22 hours, namely obtain rhodotorula glutinis seed liquor;
(3) Semen Maydis flour and wheat bran is weighed respectively according to the ratio of mass ratio 1:3, add water after mixing, make water content control for 65��68%, when 0.15MPa after sterilizing 30 minutes, it is cooled to 27��29 DEG C, access the aspergillus oryzae seed liquor of 5% and the rhodotorula glutinis seed liquor of 5%, mix thoroughly, tiling is to tray, thickness 5��8 centimetres, cover sterile gauze above, put in growth cabinet and cultivate, cultivation temperature 27��29 DEG C, growth cabinet relative humidity 90��95%, incubation time 5��6 days, it is placed under sunlight after fermentation ends to be exposed to the sun fermentation material to moisture less than 10%, fermented feed powder is obtained after pulverizing with pulverizer again, feed grade lipase is added according to the ratio of 3��4 units of every gram of fermented feed powder, stir and get product.
Priority Applications (1)
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CN201610007393.8A CN105639142A (en) | 2016-01-07 | 2016-01-07 | Production method of pig feed additive |
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CN201610007393.8A CN105639142A (en) | 2016-01-07 | 2016-01-07 | Production method of pig feed additive |
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CN105639142A true CN105639142A (en) | 2016-06-08 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114032188A (en) * | 2021-12-23 | 2022-02-11 | 杭州保安康生物技术有限公司 | Preparation method of rhodotorula glutinis culture |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102396655A (en) * | 2011-11-29 | 2012-04-04 | 淮安市动物疫病预防控制中心 | Livestock and poultry feed added with germinated brown rice |
CN104171408A (en) * | 2014-08-02 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Method for preparing laying-hen feed additive by using potato residues |
CN104171410A (en) * | 2014-08-03 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Making method of milk cow feed additive |
CN104186918A (en) * | 2014-07-29 | 2014-12-10 | 哈尔滨伟平科技开发有限公司 | Preparing method of corn gluten polypeptide for fodder |
CN104886523A (en) * | 2015-05-25 | 2015-09-09 | 哈尔滨伟平科技开发有限公司 | A delicate flavor enhancing seasoning and a manufacturing method thereof |
-
2016
- 2016-01-07 CN CN201610007393.8A patent/CN105639142A/en not_active Withdrawn
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102396655A (en) * | 2011-11-29 | 2012-04-04 | 淮安市动物疫病预防控制中心 | Livestock and poultry feed added with germinated brown rice |
CN104186918A (en) * | 2014-07-29 | 2014-12-10 | 哈尔滨伟平科技开发有限公司 | Preparing method of corn gluten polypeptide for fodder |
CN104171408A (en) * | 2014-08-02 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Method for preparing laying-hen feed additive by using potato residues |
CN104171410A (en) * | 2014-08-03 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Making method of milk cow feed additive |
CN104886523A (en) * | 2015-05-25 | 2015-09-09 | 哈尔滨伟平科技开发有限公司 | A delicate flavor enhancing seasoning and a manufacturing method thereof |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114032188A (en) * | 2021-12-23 | 2022-02-11 | 杭州保安康生物技术有限公司 | Preparation method of rhodotorula glutinis culture |
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