CN115226388A - Functional fermented green tea composite probiotic preparation and preparation method thereof - Google Patents
Functional fermented green tea composite probiotic preparation and preparation method thereof Download PDFInfo
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- CN115226388A CN115226388A CN202180002750.0A CN202180002750A CN115226388A CN 115226388 A CN115226388 A CN 115226388A CN 202180002750 A CN202180002750 A CN 202180002750A CN 115226388 A CN115226388 A CN 115226388A
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Abstract
The invention relates to a functional fermented green tea composite probiotic preparation with excellent balance force on enzyme activity and viable count and a preparation method thereof.
Description
Technical Field
The invention relates to a functional fermented green tea composite probiotic preparation, in particular to a functional fermented green tea composite probiotic preparation with excellent balance force on enzyme activity and viable count and a preparation method thereof.
Background
Generally, antibiotics are often used as a growth substance for feeding during this period in order to prevent diseases of livestock and the efficiency of raising of livestock, but such abuse of antibiotics gradually causes environmental pollution. Therefore, in the animal husbandry recently, in order to realize green breeding, antibiotics are totally prohibited, and the use of antibiotics is regulated, cannot be used for promoting growth, and can be used only for treatment, so that the finding of antibiotics substitute is urgently needed. Further, in recent years, awareness of people has been changing, and importance of microbial agents, fermented feeds, and the like, which utilize natural functional substances and effective microorganisms from the viewpoint of disease prevention, improvement of safe animal product demand, high-end animal product demand, branding demand, and animal product environment, and the like, has been attracting attention.
In order to find a growth substance that can replace antibiotics, studies have been consistently conducted on various natural substances, and particularly, the results of studies to double the efficacy by fermentation processes have recently been reported. Plants with excellent biological activity are subjected to various reactions by microorganisms by the fermentation Process, as a result of which the biological activity is enhanced at least 1-fold to at most 13-fold (Process Biochemistry40:2757-2762 lin et al,2005 plant Food for Human nutrition61, satoet et al, 2006.
Microbial feed additives are reported to balance the intestinal microbial flora of animals, to have beneficial effects on animals, to derive an intestinal preponderance of beneficial microbes when used as a microbial preparation or as a replacement for antibiotics, to enhance the health of animals, and to promote their growth (fULLER, 1989). It has been reported that when a microbial preparation is added to a feed, not only diarrhea can be prevented, but also the feeding efficiency of piglets can be improved (Pollman et al, 1980, collington et al, 1988), and the effect of improving the growth performance is exhibited not only in fattening and fattening of piglets but also in continuous-feeding pigs (Baird, 1977). Moreover, the addition of microorganisms can activate enzymes in the intestinal tract and increase the utilization of nutrients in the feed (Collinson et al, 1988, scheuermann, 1993), and also can increase the immunocompetence and enhance the health of animals (Kato et al, 1983, fuller, 1989.
In addition, the main bioactive substance of green tea is catechin (polyphenol), which is a kind of polyphenol (which accounts for more than 70% of the polyphenol content of green tea) (Khokhar and Magnusdottir, 2002), and there are flavanols (flavanols) of flavonoiddhk and its derivatives, flavanols (flavanols), flavanones (flavanones), anthocyanidines (anthocyanidines), flavonoids (flavanones), polyphenolic acids (poliglenocolic acids), etc. (Graham, 1992), the active ingredients of green tea have antioxidant effects (Kim et al, 2004 robak and gryglewski, 1998) and antibacterial power (Shi, 2002), cholesterol lowering effects (Yang and Koo,1997 jin et al, 2004), and according to the results of recent human studies, cancer preventing effects (Yang and laud, 2002) have been reported, in addition, that the total level of LDL and cholesterol can be lowered (Davies et al, 2003).
Generally, pork is a food which is preferred by consumers because it is inexpensive compared to beef and contains high-quality protein. Recently, as income levels increase, consumers have paid more attention to the quality of meat than to the quantity thereof. With the implementation of the free import policy of all animal products, the wave of import is further intensified, and the world gradually moves to a single market system. Therefore, the production of livestock products is urgently needed to reduce the production cost and have a gap with the import meat with low price through high-end quality. In order to survive the competition with imported meat, there is an urgent need to produce inexpensive, excellent quality, hygienic, and safe branded meat.
The green tea used in the present invention is a species of favorite tea that is drunk from around 2,700 years before the first yuan in china, korea, japan, and the like. Recently, with the development of pharmacological mechanisms of various components contained in green tea, people are newly recognizing their values, and in particular, polyphenols (EC, egC, ECg, egCg) are one of the main components of green tea, which are known to have various effects beneficial to human health, such as anticancer, prevention of oxidation based on active oxygen in the antioxidant organism, reduction of blood glucose, reduction of cholesterol in blood, prevention of food corrosion, and the like. In addition, green tea has effects of environmental hormone inhibitory effect, escherichia coli O-157 and food poisoning bacteria inhibitory effect, weight reducing effect, skin beautifying effect, alcohol and tobacco detoxification releasing effect, etc., and is widely known as a nutritional and delightful food. Green tea, as a non-alcoholic beverage, is a nutritious and tasty food, and also is a food having the function of regulating the functions of the human body from the viewpoint of regulating physiological functions and delivering nutrients in the aspect of preventing and treating human diseases. With the development of modern life science and technology, the pharmacological effect of the green tea is more obvious. Catechins have the effect of enhancing vascular functions, and have been studied for many years for various biological activities, among which, catechins have various pharmacological effects, i.e., a blood pressure lowering effect, an antioxidant effect, a carcinogenic inhibitory effect, an HIV reverse transcriptase inhibitory effect, an inhibition of cholesterol reuptake, and prevention of dental caries, and have been reported. The efficacy of the platelet aggregation disorder activity has been scientifically proven by reports and the like, and it is known that the utility thereof as a natural substance is high.
In view of the above, the present inventors have used the above as an introduction point to prepare a functional fermented green tea complex probiotic preparation by biological engineering using effective microorganisms.
[ Prior Art document ]
[ patent document ]
Disclosure of Invention
Problems to be solved
The invention aims to provide a functional fermented green tea compound probiotic preparation for promoting the growth of livestock, preventing diseases, inhibiting the growth of pathogenic microorganisms and promoting the propagation of beneficial bacteria and a preparation method thereof.
Another object of the present invention is to provide a feed comprising a fermented green tea complex probiotic preparation, which can significantly improve the amount of fattening and feed efficiency and enhance the productivity within the same feeding period.
The technical problem to be solved by the present invention is not limited to the above-mentioned problems, and a person skilled in the art can clearly understand other problems not mentioned by the following description.
Means for solving the problems
In order to achieve the above objects, one embodiment of the present invention provides a functional fermented green tea composite probiotic preparation, which is prepared by inoculating and fermenting Bacillus subtilis, aspergillus oryzae, lactobacillus acidophilus, streptococcus thermophilus and yeast (Saccharomyces cerevisiae) on a culture medium including green tea, red ginseng meal, soybean meal, corn, wheat bran and syrup.
According to an embodiment of the present invention, the fermentation is performed at a low temperature of 35 to 40 ℃ for 50 hours or more, and may be repeatedly performed twice or more.
According to an embodiment of the present invention, for 100 parts by weight of the culture medium, 10 to 30 parts by weight of green tea, 5 to 30 parts by weight of red ginseng meal, 30 to 50 parts by weight of soybean meal, 10 to 30 parts by weight of corn, 10 to 30 parts by weight of wheat bran, and 1 to 10 parts by weight of syrup may be used.
According to another embodiment of the present invention, there is provided a feed comprising the functional fermented green tea complex probiotic preparation.
According to still another embodiment of the present invention, there is provided a method for preparing a functional fermented green tea composite probiotic preparation, including: on culture medium containing green tea, ginseng radix Rubri cake, soybean cake, semen Maydis, testa Tritici and syrup at a ratio of 1 × 10 10 A fermentation step of fermenting anaerobic and aerobic lactic acid bacteria at 35 to 40 ℃ for 50 hours after basic spraying and inoculation of Bacillus subtilis, aspergillus oryzae, lactobacillus acidophilus, streptococcus thermophilus, and saccharomycetes, thereby preparing a culture; and on the prepared culture, 1X 10 respectively 12 Spraying and inoculating yeast (Saccharomyces cerevisiae) KCTC 7915 bacteria, bacillus subtilis KCTC 3239 bacteria, and Bacillus coagulans KCTC 101 bacteria on a/ml basisAnd 5, drying at 35-40 ℃ after bacteria are cultured until the water content reaches 10-20%.
According to an embodiment of the present invention, the fermentation step may be performed more than twice.
According to yet another embodiment of the present invention, there is provided a functional fermented green tea composite probiotic formulation prepared by the method. .
And, according to still another embodiment of the present invention, there is provided a feed including the complex probiotic formulation.
Effects of the invention
The functional fermented green tea composite probiotic preparation is rich in beneficial enzymes such as Amylase (Amylase), protease (Protease), cellulase (cellulose), phytase (Phytase) and the like secreted in the fermentation and metabolism process, and can promote the decomposition of starch, protein and plant cellulose.
In addition, the functional fermented green tea compound probiotic preparation can secrete natural antibacterial substances with antibacterial power, and has the effects of inhibiting the growth of pathogenic microorganisms such as pathogenic escherichia coli and salmonella.
In addition, the functional fermented green tea compound probiotic preparation provided by the invention is used as a fermentation metabolite, comprises natural flavoring agents such as nucleic acid and glutamine, and has the effect of enhancing the aerobic property.
In addition, the functional fermented green tea compound probiotic preparation has the effects of inhibiting the growth of harmful bacteria, promoting the reproduction of the beneficial bacteria, forming and protecting normal intestinal flora.
In addition, when the feed containing the functional fermented green tea compound probiotic preparation is used for feeding livestock, harmful gases such as ammonia, nitrous acid, hydrogen sulfide and the like can be reduced, and the feed has the effects of improving defecation state, preventing stool from deforming, further deodorizing and improving the surrounding environment.
When the feed containing the functional fermented green tea compound probiotic preparation is used for feeding livestock, the feed has the effects of stimulating immune organs of the livestock, promoting antibody formation, reducing the number of somatic cells and activating macrophages, and can finally enhance the immunity of the livestock.
Drawings
FIG. 1 shows an actual photograph of equipment used in one embodiment of the present invention.
FIG. 2 shows a medium used in one embodiment of the present invention.
FIG. 3 shows a first fermenter used in an embodiment of the present invention.
Fig. 4 illustrates a first dryer used in an embodiment of the present invention.
FIG. 5 shows a second fermenter used in an embodiment of the present invention.
Fig. 6 illustrates a second dryer used in an embodiment of the present invention.
FIG. 7 illustrates a product BIN used in accordance with an embodiment of the present invention.
FIG. 8 illustrates a shredder utilized in an embodiment of the present invention.
Fig. 9 illustrates a package utilized in accordance with an embodiment of the present invention.
FIG. 10 illustrates a warehouse of products and materials utilized in an embodiment of the present invention.
* Reference signs
Detailed Description
The present invention will be described in detail below. First, the terms or words used in the present specification and claims should not be construed as limited to dictionary meanings, but interpreted as based on meanings and concepts conforming to the technical idea of the present invention which are adopted in principle by properly defining the concept of the terms in order for the inventor to describe his invention in the most preferable way. It should be understood that the technical configuration described in the embodiments described in the present specification is only a preferred embodiment of the present invention and does not represent all the technical ideas of the present invention, and therefore, when the present invention is applied, there may be a plurality of equivalents and modifications that can be substituted therefor.
One embodiment of the present invention provides a functionally fermented green tea complex probiotic preparation, which is prepared by inoculating beneficial bacteria, more specifically, bacillus subtilis, aspergillus oryzae, lactobacillus acidophilus, streptococcus thermophilus, and yeast (Saccharomyces cerevisiae) on a medium including green tea, red ginseng meal, soybean meal, corn, wheat bran, and syrup, and fermenting.
In this case, the culture medium used in the present invention may include green tea, red ginseng meal, soybean meal, corn, wheat bran, syrup, and, if necessary, grains, bran, vegetable oil meals, animal feed materials, other feed materials, fine materials, and the like.
The green tea adopted by the invention can be directly dried green tea leaves or green tea powder which is pulverized.
The red ginseng dregs adopted by the invention are dregs left after the red ginseng components are extracted, and in consideration of economic aspects, the whole red ginseng is hardly used as a feed additive, but when the red ginseng dregs are prepared by using red ginseng extracts, the red ginseng dregs can be used as the feed additive because the red ginseng dregs are very economical and practical. In addition, the red ginseng meal is rich in dietary fiber, and also contains partial components, taste and flavor of red ginseng, so that the red ginseng meal is an excellent material. In addition, the red ginseng meal still contains a considerable amount of acidic polysaccharides having excellent biological activities, and contains various carbohydrates in addition to the acidic polysaccharides, thereby promoting the growth of microorganisms. In addition, when the culture medium comprises red ginseng meal, the culture efficiency can be improved by 30 to 40 percent, and the amino acid in the yeast is increased together. Therefore, the red ginseng meal as a byproduct obtained in the red ginseng extraction process remains various bioactive substances, which can be used as a feed additive for livestock, and can enhance the efficacy thereof through a fermentation process, thereby improving the feeding efficiency of livestock.
The soybean meal used in the present invention is a product remaining after soybean oil is extracted from soybeans, is rich in plant protein, and can be an excellent plant protein source when used as a feed for livestock.
When the corn is adopted, the corn can be dried and separated to be used, or the separated corn can be crushed and pulverized to be used.
The wheat bran adopted by the invention is the residue left after the wheat bran is processed into flour and sieved, and can become an excellent protein source like the soybean meal.
The syrup used in the present invention is black juice remaining after sugar extraction from sugar beets or sugar canes, and can be used as a fermentation raw material.
Furthermore, the culture medium may further be used in the following cereals: such as, for example, black mustard, wheat, barley, oats, rye, brown rice, buckwheat, millet, sorghum, finger millet, and the like, without limitation.
In addition, the culture medium can further use the following bran varieties: such as defatted rice bran, wheat bran, lower wheat flour, wheat, germ, barley bran, screened impurities, particulates, corn bran, corn germ, and the like, without limitation.
In addition, plant oil meals which can be further applied to the culture medium are as follows: such as bean flour, flax meal, cottonseed meal, peanut meal, safflower meal, coconut meal, palm meal, flax meal, sunflower meal, rapeseed meal, wood cottonseed meal, mustard meal, and the like, without limitation.
In addition, animal feeds which can be further applied to the culture medium are as follows: for example, fish meal (north ocean fish meal, imported fish meal, whole fish meal, yanan fish meal), dried fish floss, meat meal, fish bone meal, blood meal, decomposition product, bone meal, by-product of livestock processing, feather meal, silkworm, skim milk powder, casein, dry whey powder, etc., without limitation.
In addition, other feed raw materials which can be further applied to the culture medium are as follows: examples of the plant stems and leaves (alfalfa, hay cake, alfalfa leaf powder, locust bean-like powder, etc.), corn processing industry by-products (corn bran, wheat, corn bran, corn pancake, etc.), starch processed products, white sugar, fermentation industry products (yeast, beer cake, malt root, alcohol cake, soy sauce cake, etc.), agricultural product production by-products (citrus processed cake, bean curd cake, coffee cake, cacao bean cake, etc.), and other products (cassava, lima bean, guar bean, seaweed, antarctic shrimp, spirulina, chlorella, minerals, etc.) are not limited.
Further, the culture medium may further employ fine substances: examples of the inorganic salt include, but are not limited to, proteins (casein, albumin, etc.), amino acids, saccharides (starch, cellulose, sugar, glucose, etc.), minerals, and vitamins.
Meanwhile, the inventor verifies that after repeated experiments and operation failures: when the culture medium is prepared, if green tea, red ginseng meal, soybean meal, corn, wheat bran and syrup are mixed and used to be included, various substances can play a role in controlling balance through interaction, and finally, the heat resistance and the bacterial activity can reach the maximum.
Also, according to an embodiment of the present invention, for 100 parts by weight of the culture medium, 10 to 30 parts by weight of green tea, 5 to 30 parts by weight of red ginseng meal, 30 to 50 parts by weight of soybean meal, 10 to 30 parts by weight of corn, 10 to 30 parts by weight of wheat bran, and 1 to 10 parts by weight of syrup are used, and preferably, 15 parts by weight of baocheng green tea, 10 parts by weight of red ginseng meal, 40 parts by weight of soybean meal, 15 parts by weight of corn, 15 parts by weight of wheat bran, and 5 parts by weight of syrup may be used. When the range is satisfied, as described above, the heat resistance and the activity of bacteria can be maximized, the propagation of beneficial bacteria can be promoted, the immune organs of livestock can be stimulated, the formation of antibodies can be enhanced, the number of somatic cells can be reduced, and macrophages can be activated.
In the invention, the Bacillus subtilis is a spore-forming strain, has strong heat resistance and has excellent viability under a high-temperature condition. In addition, when the culture medium is used, the production capacity of enzymes such as amylase, protease and the like can be improved, and antibiotic and antibacterial substances can be produced to synthesize vitamin B group and produce organic acid.
In the present invention, aspergillus oryzae (Aspergillus oryzae) is a useful mold that has long been a contributor to human beings, and exerts excellent amylolytic and proteolytic functions through secretion of enzymes. In addition, when used as a culture medium, it can produce organic acids such as citric acid, acetic acid, lactic acid, etc., and stabilize pH in the gastric acid of livestock in the later stage. And moreover, antibacterial metabolites can be generated, the propagation of harmful microorganisms is inhibited, the generation of ammonia is inhibited, the fermentation of animal manure is promoted, the environment of a breeding shed is improved, and high-end organic matters are changed into fertilizers.
In the invention, lactobacillus acidophilus (Lactobacillus acidophilus) is a Lactobacillus belonging to probiotics, has acid resistance, and has excellent effects of regulating the intestines, resisting cancers, reducing blood cholesterol and synthesizing vitamin B group.
In the present invention, streptococcus thermophilus (Streptococcus thermophilus) contributes to the propagation of lactic acid bacteria and the suppression of harmful bacteria.
In the present invention, yeast (Saccharomyces cerevisiae) is rich in proteins and trace minerals, and when used as a feed, it is easily digested and absorbed in the intestinal tract of livestock to exert its effect. Further, according to an embodiment of the present invention, yeast can be used as a yeast culture rich in metabolic products, so that the yeast culture can be added, thereby greatly improving the production efficiency of animal products.
In addition, since yeast (Saccharomyces cerevisiae) contains nucleic acid, it contains nucleic acid salts, glutaminic acid, and the like during the growth of yeast, and thereby enhances the aerobic property.
Meanwhile, the saccharomycetes (saccharomycetes) have strong aerobic property, can remove oxygen in intestinal tracts, activate the reproduction of anaerobic microorganisms, generate various digestive enzymes, and enhance the digestion and absorption of the feed, thereby improving the efficiency of the feed.
In addition, yeast (Saccharomyces cerevisiae) plays a role in buffering pH, can generate vitamin B combination, UGF, amino acids and the like, and improves the fertility rate and the hatchability of livestock.
Meanwhile, the inventor verifies that the best strain combination which can obtain the strongest improvement effect through complementation is as follows through repeated experiments and operation failures: when Bacillus subtilis, aspergillus oryzae, lactobacillus acidophilus, streptococcus thermophilus and Saccharomyces cerevisiae are mixed and used simultaneously.
In addition, in one embodiment of the present invention, after inoculating Bacillus subtilis, aspergillus oryzae (Aspergillus oryzae), lactobacillus acidophilus (Lactobacillus acidophilus), streptococcus thermophilus (Streptococcus thermophilus) and yeast (Saccharomyces cerevisiae) to the above-mentioned culture medium, the medium is fermented at a low temperature of 35 to 40 ℃ for 50 hours or more, and when the fermentation is performed, it is preferably entirely placed in a large scale fermentation machine and fermented 2 or more times. Thus, the total number of colonies is kept high by repeating fermentation, metabolites are sufficiently secreted, and the cells are sporulated by low-temperature baking, which results in high heat resistance so that there is no problem in the subsequent heat treatment process for preparing a feed.
Meanwhile, still another embodiment of the present invention may provide a feed containing the fermented green tea complex probiotic preparation.
The present invention will be described in further detail below with reference to examples and experimental examples. However, these examples and experimental examples are intended to illustrate the present invention, and the scope of the present invention is not limited by these examples and experimental examples.
Example 1 functional fermentation of Green tea Complex probiotic preparation
15% by weight of baocheng green tea, 10% by weight of red ginseng meal, 40% by weight of soybean meal, 15% by weight of corn, 15% by weight of wheat bran and 5% by weight of syrup were mixed to prepare a medium having a total water content of 70%. Then, the mixed medium was placed in an environment at a temperature of 100 ℃ or higher, and sterilized for 30 minutes. The sterilized medium was used at 1X 10 in the medium shown in FIG. 2 10 On a per ml basis, bacillus subtilis, aspergillus oryzae (Aspergillus oryzae), lactobacillus acidophilus (Lactobacillus acidophilus), streptococcus thermophilus (Streptococcus thermophilus) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) purchased from the happy society of the chun bio-industry were sprayed and inoculated so that the water content of the whole mixture reached about 40%, and a functional culture was prepared by fermenting anaerobic and aerobic lactic acid bacteria at 37 ℃ for 50 hours using a fermenter as shown in fig. 3. Then, on the prepared culture, 1X 10 cells were added 12 On a per ml basis, yeast (Saccharomyces cerevisiae) KCTC 7915, bacillus subtilis KCTC 3239 and Bacillus coagulans KCTC 1015, which were purchased from Hakka biosciences, chunchuan biological industry, were sprayed and inoculated, and then, a dryer shown in FIG. 4 was used,drying at 37 deg.C for 36 hr until the water content reaches 20%. And (3) performing a secondary fermentation process of fermenting for 50 hours at the temperature of 37 ℃ by using a fermentation machine shown in figure 5 for the dried product, and drying for 36 hours at the temperature of 37 ℃ by using a drying machine shown in figure 6 for the product of secondary fermentation until the water content reaches 15%, thereby preparing the functional fermented green tea compound probiotic preparation.
Example 2
The feed containing 0.3% of the functional fermented green tea composite probiotic preparation of example 1 was prepared. Figures 7 to 10 show the mechanical means actually used in the preparation of the feed.
Evaluation example 1
On 11/14/2019, the feed of example 2 was examined by the institute for feed of Korean Single ingredient feed Association (accession number: GI 11-154), and as shown in Table 1 below, no harmful bacteria such as Salmonella were detected at all, and the feed was found to be rich in beneficial bacteria such as Bacillus and lactic acid bacteria.
[ TABLE 1 ]
| Delegation of detection components | Unit | The result of the detection | Detection method |
| Salmonella | Not detected out | Standard analysis method for feed | |
| Bacillus subtilisBacteria (Bacillus subtilis) | cfu/g | 1.4X10 7 | Standard analysis method for feed |
| Lactobacillus acidophilus (Lactobacillus acidophilus) | cfu/g | 5.8X10 7 | Standard analysis method for feed |
| Streptococcus thermophilus (Streptococcus thermophilus) | cfu/g | 2.0X10 7 | Standard analytical method for feed |
| Aspergillus oryzae (Aspergillus oryzae) | cfu/g | 2.8X10 7 | Standard analysis method for feed |
| Yeast (Saccharomyces cerevisiae) | cfu/g | 7.7X10 8 | Standard analysis method for feed |
Evaluation example 2
The feed of example 2 was examined on 22.1.2019 by the Korean feed society institute of feed technology (accession number: 190110-15), and mycotoxins were not detected at all, and the results are shown in Table 2 below.
[ TABLE 2 ]
| Mycotoxins | The result of the detection | Detection method |
| Aflatoxin (ppb) | Undetected | Feed Standard analysis 7.1.LA |
| Ochratoxins (ppb) | Not detected out | Feed Standard analysis 7.3.NA |
Evaluation example 3
As shown in table 3 below, pigs and cattle were fed after preparing feeds containing a certain proportion of the functionally fermented green tea complex probiotic preparation.
[ TABLE 3 ]
| Livestock breeding | Content of functional fermented green tea composite probiotic preparation |
| Brood and laying hen | 0.1～0.2％ |
| Breeder and broiler chickens | 0.1～0.2％ |
| Piglets and sucking pigs | 0.2％ |
| Growing and fattening pig | 0.1％ |
| Sow (female pig) | 0.1～0.2％ |
| Calf | 0.2％ |
| Fat beef | 0.1％ |
| Dairy cow | 0.1～0.2％ |
At this time, it was confirmed that the laying rate of the laying hen poultry is improved, and as the weight of the eggs and the eggshell are improved, the shell is prevented from being thin and fragile. When the breeding hens are fed, the hatching rate can be improved, and the quality of hatching eggs is improved.
Proved by experiments, when the broiler chicken is fed with the feed, the feed can enhance the bone development, improve the daily weight gain rate, prevent diarrhea and the like.
The feed can improve the digestibility and the conception rate and the number of born pigs when being fed to pigs (piglets, grown pigs, fattening pigs and sows), can prevent diarrhea, increase the weight, increase the intake and daily gain when being fed to weaned piglets, and can further reduce the death rate, prevent digestive system dysfunction, reduce abortion, stillbirth and the like when being fed to suckling piglets.
In addition, through verification, when the feed is fed to cattle (calves, fat cattle and dairy cows), the feed intake and feed efficiency can be improved, the digestibility can be improved, the growth can be promoted, and the weaning period can be shortened. In addition, pH, propionic acid (propionic acid), gastric juice flow rate (flow rate) and the like in the ruminants are improved, and the milk yield and the milk maintenance amount are increased. Meanwhile, the total amount of anaerobic microorganisms and cellulose decomposing microorganisms is increased, the digestibility of crude protein and crude fiber is improved, and the number of somatic cells in a body is reduced.
Moreover, it is proved that livestock fed by the feed containing the functional fermented green tea composite probiotic preparation in the embodiment of the invention have the functions of inhibiting pathogenic microorganisms (pathogenic escherichia coli, salmonella and the like) and removing Aflatoxin (Aflatoxin) in the feed, the adaptability of discomfort caused by conveying and environmental change is enhanced, harmful gases such as ammonia gas and the like in a feeding shed are obviously reduced, the production of high-quality organic fertilizers is facilitated, and the immunity is enhanced.
The above-described embodiments of the present invention are intended to illustrate the present invention, and it should be understood that a person skilled in the art to which the present invention pertains can easily modify the present invention into other specific forms without changing the technical idea or essential features of the present invention. For example, various components described as a single form may be implemented by a dispersed form, and similarly, components described as a dispersed form may be implemented by a combined form.
The scope of the present invention is defined by the appended claims, and all changes and modifications derived from the meaning and scope of the claims and equivalents thereof should be construed as falling within the scope of the present invention.
Claims (4)
1. A functional fermented green tea composite probiotic preparation is characterized in that: it is prepared by inoculating beneficial bacteria to culture medium containing green tea, red ginseng cake, soybean cake, corn, wheat bran and syrup, and fermenting.
2. The functional fermented green tea complex probiotic formulation according to claim 1, characterized in that: the fermentation is carried out at a low temperature of 35 ℃ to 40 ℃ for more than 50 hours, and is repeatedly carried out for more than two times.
3. The functional fermented green tea complex probiotic formulation according to claim 1, characterized in that: for 100 parts by weight of the culture medium, 10 to 30 parts by weight of green tea, 5 to 30 parts by weight of red ginseng meal, 30 to 50 parts by weight of soybean meal, 10 to 30 parts by weight of corn, 10 to 30 parts by weight of wheat bran and 1 to 10 parts by weight of syrup are adopted.
4. A feed comprising the functional fermented green tea complex probiotic formulation of any one of claims 1 to 3.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2020-0175372 | 2020-12-15 | ||
| KR1020200175372A KR20220085367A (en) | 2020-12-15 | 2020-12-15 | Functional fermemted green tea complex probiotic and manufacturing method thereof |
| PCT/KR2021/012817 WO2022131493A1 (en) | 2020-12-15 | 2021-09-17 | Functional fermented-green tea composite probiotic, and production method for same |
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| Publication Number | Publication Date |
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| CN115226388A true CN115226388A (en) | 2022-10-21 |
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| CN202180002750.0A Pending CN115226388A (en) | 2020-12-15 | 2021-09-17 | Functional fermented green tea composite probiotic preparation and preparation method thereof |
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| Country | Link |
|---|---|
| KR (1) | KR20220085367A (en) |
| CN (1) | CN115226388A (en) |
| WO (1) | WO2022131493A1 (en) |
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| KR101256793B1 (en) | 2011-11-04 | 2013-04-19 | 주식회사 이앤티 | Feed additive for piglet, and processing method of it |
| CN104472879A (en) * | 2014-12-22 | 2015-04-01 | 福建农林大学 | Tea residue feed additive and preparation method thereof |
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| WO2022131493A1 (en) | 2022-06-23 |
| KR20220085367A (en) | 2022-06-22 |
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