CN102669485A - Animal biotin special for broilers and preparing method thereof - Google Patents
Animal biotin special for broilers and preparing method thereof Download PDFInfo
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- CN102669485A CN102669485A CN2012101888533A CN201210188853A CN102669485A CN 102669485 A CN102669485 A CN 102669485A CN 2012101888533 A CN2012101888533 A CN 2012101888533A CN 201210188853 A CN201210188853 A CN 201210188853A CN 102669485 A CN102669485 A CN 102669485A
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- 241000287828 Gallus gallus Species 0.000 title abstract description 9
- 238000000034 method Methods 0.000 title abstract description 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 title abstract 10
- 229960002685 biotin Drugs 0.000 title abstract 5
- 235000020958 biotin Nutrition 0.000 title abstract 5
- 239000011616 biotin Substances 0.000 title abstract 5
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- 238000002360 preparation method Methods 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 22
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- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 21
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Disclosed is animal biotin special for broilers and a preparing method thereof. The animal biotin is characterized in that the preparation materials comprise the components of feed grade fulvic acid, dandelion general flavones and fenugreek meal fermentation culture; the fenugreek meal fermentation culture comprises fenugreek substrate and inoculated liquid, wherein the fenugreek substrate comprises fenugreek meal, corn flour and beet molasses; and the inoculated liquid comprises saccharomyces cerevisiae, lactobacillus plantarum, bacillus subtillis and bacillus licheniformis, wherein the total quantity is 10x10<8> cfu/g, the total quantity of lactobacillus plantarum and bacillus subtillis is 5x10<8> cfu/g, saccharomyces cerevisiae accounts for 3x10<8>cfu/g, and lactobacillus plantarum accounts for 2x10<8> cfu/g. According to the animal biotin special for broilers, digestive systems and immune systems of animals can be repaired, regulated and stimulated to achieve effects of growth promotion, immunity enhancing, disease prevention and resisting and improvement of animal product quality. The animal biotin special for broilers is a natural green product without any hormone.
Description
Technical field
The present invention relates to a kind of animal life element and preparation method thereof, particularly relate to a kind of fryer tailored version animal life element and preparation method thereof.
Background technology
Along with the attention of the mankind to food security, the antibiotic use of Western medicine is more and more in the fryer limit, and existing feed kind and function are all fewer, do not possess the function of adjusting maincenter immunity, and the scope of application is narrower, is unfavorable for promoting the use of.Since the forties in last century, antibiotic came out, antibiotic had obtained extensive use, and obvious facilitation is played in sick prevention of chicken and treatment, had brought considerable economic for numerous raisers.More antibiotic side effects also show, and affect the further raising of meat chicken production performance but simultaneously.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body; Can be distributed to each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone with blood circulation; Antibiotic can cause antigenic quality to reduce; Directly influence immunologic process, thereby the inoculation of vaccine is produced harmful effect, the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can influence in the body profitable strain growth simultaneously acting on pathogen; Especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys; The harmful bacterium that hides in vivo takes advantage of the occasion to breed in a large number, and causes endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroy microecological balance, and one or more are endogenous in addition or the external source germ infects once more immediately that body causes.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most of antibiotics is excreted by ight soil, also can remain in the milk through the lactation process.After being excreted in the environment, the antibiotic of some stable in properties still can have a very long time; Thereby cause the medicament residue in the environment; These remaining medicines are slowly accumulated in human body and other plant body through livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium; Lose resistance, or body is produced toxic action because of accumulating in a large number to some disease.
Summary of the invention
It is plain and preparation method thereof to the purpose of this invention is to provide a kind of fryer tailored version animal life, overcomes effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.The animal life element of our company's invention is to be composited through the science compatibility to have probio, multiple Chinese herbal medicine and other nutriment that can improve functions of intestines and stomach and improve the animal immune function.Can reach growth promotion through digestive system, the immune system of repairing, regulating, exciting animal body self, improve immunity, disease resistance, improve the effect of animal product quality.These article are not for containing the natural green product of any hormone.
For realizing above-mentioned purpose; The invention provides a kind of fryer tailored version animal life element and preparation method thereof, its preparation raw material comprises following constituent: feed grade fulvic acid 35%-60%, dandelion general flavone 0%-0.4%, bitter bean pulp fermentation culture 30%-65%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-15%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8-15 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 2 * 10
8-7 * 10
8Cfu/g, S. cervisiae 1 * 10
8-6 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8-4 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
It is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
The bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
The invention provides a kind of fryer tailored version animal life element and preparation method thereof, have the following advantages:
Add Lactobacillus plantarum and can effectively available essential amino acid and various vitamin (vitamin B complex and K etc.) be provided for the host; Also can improve the biologically active of mineral element, and then be reached for that fryer provides essential nutriment, strengthens the Nutrition and Metabolism of animal, the directly effect of short its growth.Lactobacillus plantarum is the obviously phagocytosis of activating macrophage on the one hand, on the other hand because it can be equivalent to natural active immunity in the enteron aisle field planting.They can also stimulate peritoneal macrophages, induce the generation interferon, promote cell division, produce antibody and promote cellular immunity etc., thus the non-specific and specific immune response of ability enhancing body, the resistance against diseases of raising body.The fryer intestinal environment be slant acidity could guarantee that lactic acid bacteria can secrete organic acid by harmful bacterium invasion and attack, keep normal pH value in the enteron aisle; After adding lactic acid bacteria in addition; Can also promote gemma bacterium and saccharomycetic increment, make the beneficial bacterium status of having the advantage fast, help intestinal health.
Through embodiment, technical scheme of the present invention is done further detailed description below.
The specific embodiment
Further specify the plain technical scheme of fryer tailored version animal life of the present invention through specific embodiment below.
Embodiment 1
A kind of fryer tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 45%, dandelion general flavone 0.3%, bitter bean pulp fermentation culture 54.7%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85%, corn flour: 14%, beet molasses 1%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 5 * 10
8Cfu/g, S. cervisiae 3 * 10
8Cfu/g, Lactobacillus plantarum 2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 6: 4 (W/V).
It is brown coal, weathered coal that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal are crushed to 60 orders, and it is in 2% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 8 part by weight, in the immersion 3 hours down of 60 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 2 part by weight is 30% hydrogen peroxide solution, in temperature 70 ℃, time activation 2 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 60; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid of pH value=6.5.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 3: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 17 hours down of 20 ℃ of temperature, anaerobic fermentation is 55 hours again.
Embodiment 2
A kind of fryer tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 55%, dandelion general flavone 0.2%, bitter bean pulp fermentation culture 44.8%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 90%, corn flour: 9.5%, beet molasses 0.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 11 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 6 * 10
8Cfu/g, S. cervisiae 2 * 10
8Cfu/g, Lactobacillus plantarum 3 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 5: 5 (W/V).
It is brown coal that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal are broken to 70 orders, and it is in 3% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 9 part by weight, in the immersion 2.5 hours down of 70 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1 part by weight is 30% hydrogen peroxide solution, in 80 ℃ of following activation of temperature 1.5 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 70; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid of pH value=7.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 4: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 27 hours down of 30 ℃ of temperature, anaerobic fermentation is 45 hours again.
Embodiment 3
A kind of fryer tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 35%, dandelion general flavone 0.4%, bitter bean pulp fermentation culture 64.6%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 95%, corn flour: 3.5%, beet molasses 1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 9 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8Cfu/g, S. cervisiae 5 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 7: 3 (W/V).
It is brown coal, weathered coal, peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat are broken to 80 orders, and it is in 4% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 10 part by weight, in the immersion 2 hours down of 80 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1.5 part by weight is 30% hydrogen peroxide solution, in 75 ℃ of following activation of temperature 1.8 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 80; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid of pH value=6.8.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 2: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 34 hours down of 40 ℃ of temperature, anaerobic fermentation is 38 hours again.
In the technical scheme of plain embodiment 1 to 3 embodiment of the invention described above fryer tailored version animal life, said bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Milk cow tailored version animal life element of the present invention can admix that feeding dorking gets final product in the feed according to the ratio of 3-5 kg/ton.
Technical scheme through the plain embodiment 1 to embodiment 3 of the invention described above fryer tailored version animal life can be found out; Compared with prior art, fryer tailored version animal life element of the present invention has the remarkable result that improves functions of intestines and stomach, enhancing body immunity, safe ready, stable performance, is prone to preservation.Add Lactobacillus plantarum and can effectively available essential amino acid and various vitamin (vitamin B complex and K etc.) be provided for the host; Also can improve the biologically active of mineral element, and then be reached for that fryer provides essential nutriment, strengthens the Nutrition and Metabolism of animal, the directly effect of short its growth.Lactobacillus plantarum is the obviously phagocytosis of activating macrophage on the one hand, on the other hand because it can be equivalent to natural active immunity in the enteron aisle field planting.They can also stimulate peritoneal macrophages, induce the generation interferon, promote cell division, produce antibody and promote cellular immunity etc., thus the non-specific and specific immune response of ability enhancing body, the resistance against diseases of raising body.The fryer intestinal environment be slant acidity could guarantee that lactic acid bacteria can secrete organic acid by harmful bacterium invasion and attack, keep normal pH value in the enteron aisle; After adding lactic acid bacteria in addition; Can also promote gemma bacterium and saccharomycetic increment, make the beneficial bacterium status of having the advantage fast, help intestinal health.
Through concrete test the remarkable efficacy that fryer tailored version animal life of the present invention is plain is described below.
This test is intended to zoologize the influence of biogen to fast large-scale meat chick production performance.Select 432 of the healthy Luo Si 308 meat chicks of 1 age in days body condition for use, be divided into two processing (being designated as 1#, 2#) at random, 4 repetitions are established in each processing, and each repeats 54 chickens.The design of employing single factor experiment; Recommendation feeding standard according to this kind; In conjunction with the nutritional need of NRC (1994) meat chick, make that three stage metabolizable energy concentration are respectively 2.88,2.99,3.05Mcal/kg, crude protein content is 20.37,18.97,17.44% basal diet; In basal diet, add Bacitracin Zinc 100mg/kg+ colistin 20mg/kg; Constitute the 1# daily ration, in basal diet, add the plain 2# of formation of the animal life daily ration of 3kg/kg, give respectively and raise the examination chicken of above-mentioned two processing; Experimental period is 49 days.
Table 1 biogen is to the influence of meat chicken production performance
The result shows: the animal life element can obviously reduce the death rate (P＜0.05) of fryer, and especially in 0-21 age in days and 43-49 age in days stage, on 22-42 age in days and 43-49 age in days stage daily gain, feedstuff-meat ratio, the plain group of animal life is superior to the antibiotic group; In the whole process of meat chick was raised, the animal life element can replace Bacitracin Zinc+colistin combination, improved daily gain 2.8%, reduced material anharmonic ratio 5.6%.
It should be noted last that; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although the present invention is specified with reference to preferred embodiment; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention, and do not break away from the spirit and the scope of technical scheme of the present invention.
Claims (4)
1. fryer tailored version animal life is plain and preparation method thereof, and it is characterized in that: its preparation raw material comprises following constituent: feed grade fulvic acid 35%-60%, dandelion general flavone 0%-0.4%, bitter bean pulp fermentation culture 30%-65%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-15%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8-15 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 2 * 10
8-7 * 10
8Cfu/g, S. cervisiae 1 * 10
8-6 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8-4 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
2. fryer tailored version animal life according to claim 1 is plain and preparation method thereof, it is characterized in that: it is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
3. fryer tailored version animal life according to claim 1 is plain and preparation method thereof, and it is characterized in that: the extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
4. fryer tailored version animal life according to claim 1 is plain and preparation method thereof; It is characterized in that: the bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
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