CN107484919A - A kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof - Google Patents
A kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof Download PDFInfo
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- CN107484919A CN107484919A CN201710596355.5A CN201710596355A CN107484919A CN 107484919 A CN107484919 A CN 107484919A CN 201710596355 A CN201710596355 A CN 201710596355A CN 107484919 A CN107484919 A CN 107484919A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof, the composition and its parts by weight of the additive for microbe feedstuff are：16 22 parts of the capsule of weeping forsythia, 16 19 parts of Herba Andrographitis, 12 17 parts of sweet wormwood, 15 21 parts of rheum officinale, 12 parts of Radix Angelicae Sinensis, 13 20 parts of cordate houttuynia, 10 15 parts of radix glycyrrhizae, 7 11 parts of wilsonii, 0.6 0.9 parts of garlic, 23 parts of dried orange peel, 35 parts of the bark of eucommia, 0.8 1.3 parts of bacillus subtilis, 1 1.4 parts of Lactobacillus plantarum.Have the beneficial effect that：Additive for microbe feedstuff of the present invention, which has, to have no toxic side effect, is pollution-free, noresidue, not producing the security features such as drug resistance；Host's secretory antibody can be promoted, improve the activity of host immune cell, improve immunity of organisms and resistance；Intestinal microecology balance and optimal immune state are kept, improves the digestibility of nutriment.
Description
Technical field
The present invention relates to aquaculture feeds processing technique field, more particularly to a kind of micro- life that can strengthen fish immunity power
Thing feed addictive and application thereof.
Background technology
In the wild fish water environment higher due to being in microecosystem stability, therefore seldom morbidity；And artificial feeding
Fish are in changeable microecosystem, and the chance for causing microorganism to be lacked of proper care easily is created to condition pathogen.In this context, resist
Raw plain class feed addictive is held in both hands by heat, is widely used in feed industry, is promoted culture fishery to a certain extent
Development, but antibiotic bring high residue, high pollution and the resistance to the action of a drug the problems such as more serious.Current social, food security
Frequent Accidents, people also more focus on healthy diet while meeting and having enough to eat and wear, and green and healthy aquatic products receive much concern.At present
Existing many countries promulgate a decree in the world, forbid adding antibiotic in animal feed, to antibiotic in animal husbandry
Using controlling stricter.
Microorganism formulation refers under microecology theoretical direction, the probiotics known using oneself, by cultivating, fermenting, drying,
Special process and a kind of manufactured biological agents rich in viable bacteria such as reprocessing.Microorganism formulation can adjust microecological balance mistake
Adjust, keep microecological balance, improve the horizontal normal flora of host health and its metabolite and to selectively promote host normal
The substance preparation general name of flora growth.It covers probiotics viable bacteria and its metabolite and inactivated bacteria.Microorganism formulation is to passing
The reform and innovation of system processing technique of Chinese herbs, have various features and advantage.This technology is introduced into grinding for Chinese herbal feed additive
Study carefully, its scientific and technological content can be substantially improved, effect is more notable, and quality is more stable, and benefit is more obvious, can significantly improve
Immunity of organisms, strengthen the level of aquatic livestock resist the disease, improve function of intestinal canal, promote digestion, for solving current aquatic products
The various problems of aquatic livestock have important application value in aquaculture, and this shows for focusing on the culture fishery of aquaculture cost
Obtain particularly important.
Prior art such as Authorization Notice No. is the B of CN 103981129 Chinese invention patent, discloses a kind of composite microbial
Thing preparation and its preparation method and application, contain VREF and Kluyveromyces marxianus in the microorganism formulation, by its with
After bacillus amyloliquefaciens, jerusalem artichoke powder and food grade kieselguhr compounding, by mutual between microbial inoculum and between microbial inoculum and filler
Synergy, not only have and strengthen animal immune system, selectively promote animal intestinal tract beneficial bacterium propagation, suppress determining for harmful bacteria
Grow and breed, improve microbial environment in animal, stimulate immune response, enhancing immunity of livestock, improve Production of Livestock and Poultry
Can, antibiotic usage is reduced, has identification adhesion well to mycotoxins in feed and eliminates ability, the work(improved food conversion ratio
Energy.But the Chinese medicine microecological preparation is to the DeGrain of enhancing fish immunity power.
The content of the invention
It is an object of the invention to provide one kind can effectively strengthen fish immunity power and resistance, keep intestinal microecology to put down
Weighing apparatus and optimal immune state, the digestibility of nutriment is improved, have and have no toxic side effect, be pollution-free, noresidue, not producing
Additive for microbe feedstuff additive that can strengthen fish immunity power of the security features such as drug resistance and application thereof.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is：One kind can strengthen fish immunity power
Additive for microbe feedstuff, composition and its parts by weight are：It is capsule of weeping forsythia 16-22 parts, Herba Andrographitis 16-19 parts, sweet wormwood 12-17 parts, big
Yellow 15-21 parts, Radix Angelicae Sinensis 1-2 parts, cordate houttuynia 13-20 parts, radix glycyrrhizae 10-15 parts, wilsonii 7-11 parts, garlic 0.6-0.9 parts, dried orange peel
2-3 parts, bark of eucommia 3-5 parts, bacillus subtilis 0.8-1.3 parts, Lactobacillus plantarum 1-1.4 parts.Bacillus subtilis and plant breast bar
Bacterium is purchased from Hebei Cangzhou Zhong Xin biotechnologies company.Mentioned microorganism feed addictive is less prone to the secondary work of residual, the resistance to the action of a drug, poison
With having safety and reliability；Nospecific immunity regulatory factor can be produced in fish body, it can utilize bacterium itself
Or the composition of bacteria cell wall carrys out the immunocyte of stimulation of host, is activated, the immunocompetence of body is improved；Can significantly it increase
Add the content of body interior antibody, improve immunoglobulin concentrations, increase the activity of macrophage, and then strengthen the immunity of body
And resistance；There is antibacterium and virus, can significantly kill and suppress pathogenic microorganism, reduce fish illness
Probability, improve survival rate.
A kind of preparation method for the additive for microbe feedstuff that can strengthen fish immunity power, preparation method specifically includes following
Step：
The capsule of weeping forsythia, Herba Andrographitis, sweet wormwood, rheum officinale, Radix Angelicae Sinensis, cordate houttuynia, radix glycyrrhizae, wilsonii, garlic, dried orange peel, the bark of eucommia, powder are measured by formula
Using solid-liquid ratio as 1 after broken:5-6 adds water, adds powder weight 0.5-0.8% active peptides, is well mixed, Microwave Extraction 15-
25min, centrifugation, produces leaching liquor, the amino acid sequence of above-mentioned active peptides is
TDHKLCVCVNCFCCGAAKAFHFYKGCSLYEENVSYKPFVCIS, the active peptide have hydrophily and lipophilicity, hydrophily
It is set to be dissolved in distilled water, lipophilicity makes it be combined with powder cell membrane, makes to form aperture under cell membrane, causes intracellular thing
Matter leaks, and improves the operating efficiency of extraction, also improves the yield of extract active ingredient, and Microwave Extraction can make cell in the step
Internal temperature rises rapidly, and cellular stress exceedes the ability to bear of cell wall expansion, makes cell membrane and cellular membrane disruption or dissolving,
The active ingredient of powder is discharged, the step binding activity polypeptide Hull microwave so that extraction rate is fast, the time is short, carries significantly
The recovery rate of high effective ingredient, it can effectively protect medium-height grass the effective elements of the medicine；
Leaching liquor 95-100 parts, peptone 0.5-0.6 parts, beef extract 0.2-0.3 parts, lactose 1.2-1.5 parts, phosphorus are taken by weight
Acid dihydride sodium 0.28-0.37 parts, are mixed evenly to obtain zymotic fluid, and pH is adjusted into 6.5-7.5, sterilizing, are inoculated with formula ratio withered grass
Gemma bar and Lactobacillus plantarum, fermented 40-45h at 30-35 DEG C, produces tunning, and normal training is replaced with Chinese medicine extract
Foster base cultivates microorganism, and microorganism becomes it using protein and polysaccharide in medicinal herbs in the enzyme system degraded of itself secretion
It is easy to the amino acid, small peptide and low molecule carbohydrate digested and assimilated, and can generates during the fermentation various has anti-oxidant work(
The bioactive substance of effect so that contain polysaccharide component, probiotics and its metabolite in fermentate；
It is 5-8% that tunning is dried into water content at 30-45 DEG C, produces additive for microbe feedstuff, the microbiological feed
Additive can stimulation of host cell and activating macrophage, improve the activity of host immune cell, promote host secrete IgA,
The antibody such as IgG, IgM, immunity of organisms and resistance are improved, while bred rapidly after entering host body, enteron aisle can be reduced
PH, itself can secreting bacteria usually suppress the growths of pathogenic bacteria, be advantageous to the growth of beneficial bacteria of intestinal tract, keep intestinal microecology
Balance and optimal immune state, improve the digestibility of nutriment.
A kind of purposes for the additive for microbe feedstuff that can strengthen fish immunity power, the additive for microbe feedstuff is added
Into fish meal, fed after well mixed, addition is the 0.05-0.08% of fish meal weight, and additive for microbe feedstuff adds
Into the feed, the mouthfeel of feed can be improved, improve absorptivity and utilization rate of the fish to nutriment in feed；In fish body
It is interior to produce nospecific immunity regulatory factor and antibody, improve the immunocompetence and resistance of body；Energy after into body
It is enough significantly to kill and suppress pathogenic microorganism, the ill probability of fish is reduced, raising fish culture survival rate, improves meat,
Reduce cultivation input.
Compared with prior art, the advantage of the invention is that：Additive for microbe feedstuff of the present invention contains polysaccharide component, benefit
Raw bacterium and its metabolite, host is promoted to secrete the antibody such as IgA, IgG, IgM, being capable of stimulation of host immunocyte and activation macrophage
Cell, the activity of host immune cell is improved, improve immunity of organisms and resistance；Breed rapidly after into host body, energy
Enough reduce intestinal pH, itself can secreting bacteria usually suppress the growths of pathogenic bacteria, be advantageous to the growth of beneficial bacteria of intestinal tract, keep
Intestinal microecology balances and optimal immune state, improves the digestibility of nutriment；And have no toxic side effect, it is pollution-free,
Noresidue, the security features such as drug resistance are not produced, can be directly appended to be fed in fish feed, effect stability is reliable,
It disclosure satisfy that the requirement that people keep the safety in production to meat, egg, milk.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of additive for microbe feedstuff that can strengthen fish immunity power, composition and its parts by weight are：20 parts of the capsule of weeping forsythia, Herba Andrographitis 18
Part, 15 parts of sweet wormwood, 18 parts of rheum officinale, 1.5 parts of Radix Angelicae Sinensis, 16 parts of cordate houttuynia, 12 parts of radix glycyrrhizae, 9 parts of wilsonii, 0.7 part of garlic, dried orange peel
2.5 parts, 4 parts of the bark of eucommia, 1 part of bacillus subtilis, 1.2 parts of Lactobacillus plantarum.
A kind of preparation method for the additive for microbe feedstuff that can strengthen fish immunity power, preparation method specifically includes following
Step：
1）The capsule of weeping forsythia, Herba Andrographitis, sweet wormwood, rheum officinale, Radix Angelicae Sinensis, cordate houttuynia, radix glycyrrhizae, wilsonii, garlic, dried orange peel, the bark of eucommia are measured by formula,
Through mechanical crushing to 50 mesh, then using solid-liquid ratio as 1:5.5 add water, add the active peptides of powder weight 0.65%, and stirring is mixed
Close uniformly, Microwave Extraction 20min, be subsequently placed in centrifuge, centrifuge 16min in the case where rotating speed is 5000r/min, supernatant produces
Leaching liquor, the amino acid sequence of above-mentioned active peptides is TDHKLCVCVNCFCCGAAKAFHFYKGCSLYEENVSYKPFVCIS,
The active peptide has hydrophily and lipophilicity, and hydrophily makes it be dissolved in distilled water, and lipophilicity makes it be combined with powder cell membrane,
Make to form aperture under cell membrane, cause intracellular content leaks, improve the operating efficiency of extraction, also improve extract effectively into
Point yield, Microwave Extraction can be such that cell interior temperature rises rapidly in the step, and cellular stress exceedes holding for cell wall expansion
By ability, make cell membrane and cellular membrane disruption or dissolving, discharge the active ingredient of powder, the step binding activity polypeptide Hull
Microwave so that extraction rate is fast, the time is short, greatly improves the recovery rate of effective ingredient, can effectively protect Chinese herbal medicine
Active ingredient；
2）98 parts of leaching liquor, 0.55 part of peptone, 0.25 part of beef extract, 1.4 parts of lactose, sodium dihydrogen phosphate 0.32 are taken by weight
Part, zymotic fluid is mixed evenly to obtain, pH is adjusted to 7, the autoclaving 10min at 121 DEG C, is cooled to room temperature, inoculation formula
Bacillus subtilis and Lactobacillus plantarum are measured, is subsequently placed in the shaking table that temperature is 32 DEG C, rotating speed is 200r/min, ferment 42h, i.e.,
Tunning is obtained, replaces normal incubation medium with Chinese medicine extract to cultivate microorganism, microorganism is dropped using the enzyme system of itself secretion
Protein and polysaccharide in solution in medicinal herbs, its amino acid, small peptide and low molecule carbohydrate, and can for becoming to be easy to digest and assimilate is set to exist
The various bioactive substances with anti-oxidation efficacy are generated in fermentation process so that containing polysaccharide component, prebiotic in fermentate
Bacterium and its metabolite；
3）It is 7% that tunning is dried into water content at 38 DEG C, produces additive for microbe feedstuff, the microbiological feed adds
Add agent can stimulation of host cell and activating macrophage, improve the activity of host immune cell, promote host secrete IgA,
The antibody such as IgG, IgM, immunity of organisms and resistance are improved, while bred rapidly after entering host body, enteron aisle can be reduced
PH, itself can secreting bacteria usually suppress the growths of pathogenic bacteria, be advantageous to the growth of beneficial bacteria of intestinal tract, keep intestinal microecology
Balance and optimal immune state, improve the digestibility of nutriment.
A kind of purposes for the additive for microbe feedstuff that can strengthen fish immunity power, the additive for microbe feedstuff is added
Into fish meal, fed after well mixed, addition is the 0.06% of fish meal weight, and additive for microbe feedstuff is added to this
In feed, the mouthfeel of feed can be improved, improve absorptivity and utilization rate of the fish to nutriment in feed；Can in fish body
To produce nospecific immunity regulatory factor and antibody, the immunocompetence and resistance of body are improved；Can be bright after into body
Aobvious kill and suppression pathogenic microorganism, reduce the ill probability of fish, improve fish culture survival rate, improve meat, reduction
Cultivation input.
Embodiment 2：
A kind of additive for microbe feedstuff that can strengthen fish immunity power, composition and its parts by weight are：17 parts of the capsule of weeping forsythia, Herba Andrographitis 19
Part, 13 parts of sweet wormwood, 21 parts of rheum officinale, 1.3 parts of Radix Angelicae Sinensis, 19 parts of cordate houttuynia, 11 parts of radix glycyrrhizae, 11 parts of wilsonii, 0.7 part of garlic, dried orange peel
2.9 parts, 3.7 parts of the bark of eucommia, 1.3 parts of bacillus subtilis, 1.1 parts of Lactobacillus plantarum.
A kind of preparation method for the additive for microbe feedstuff that can strengthen fish immunity power, comprises the following steps：
The capsule of weeping forsythia, Herba Andrographitis, sweet wormwood, rheum officinale, Radix Angelicae Sinensis, cordate houttuynia, radix glycyrrhizae, wilsonii, garlic, dried orange peel, the bark of eucommia, powder are measured by formula
Using solid-liquid ratio as 1 after broken:5.2 add water, add the active peptides of powder weight 0.8%, are well mixed, Microwave Extraction 16min,
Centrifugation, produces leaching liquor, the amino acid sequence of above-mentioned active peptides is
TDHKLCVCVNCFCCGAAKAFHFYKGCSLYEENVSYKPFVCIS, the active peptide have hydrophily and lipophilicity, hydrophily
It is set to be dissolved in distilled water, lipophilicity makes it be combined with powder cell membrane, makes to form aperture under cell membrane, causes intracellular thing
Matter leaks, and improves the operating efficiency of extraction, also improves the yield of extract active ingredient, and Microwave Extraction can make cell in the step
Internal temperature rises rapidly, and cellular stress exceedes the ability to bear of cell wall expansion, makes cell membrane and cellular membrane disruption or dissolving,
The active ingredient of powder is discharged, the step binding activity polypeptide Hull microwave so that extraction rate is fast, the time is short, carries significantly
The recovery rate of high effective ingredient, it can effectively protect medium-height grass the effective elements of the medicine；
100 parts of leaching liquor, 0.58 part of peptone, 0.22 part of beef extract, 1.5 parts of lactose, sodium dihydrogen phosphate 0.2 are taken by weight
Part, zymotic fluid is mixed evenly to obtain, pH is adjusted to 6.7, sterilizing, is inoculated with formula ratio bacillus subtilis and Lactobacillus plantarum,
Ferment 45h at 30 DEG C, produces tunning, replaces normal incubation medium with Chinese medicine extract to cultivate microorganism, microorganism utilizes
Protein and polysaccharide in the enzyme system degraded of itself secretion in medicinal herbs, make amino acid, small peptide that it becomes to be easy to digest and assimilate and
Low molecule carbohydrate, and can generate the various bioactive substances with anti-oxidation efficacy during the fermentation so that fermentate
In contain polysaccharide component, probiotics and its metabolite；
3）It is 8% that tunning is dried into water content at 33 DEG C, produces additive for microbe feedstuff, the microbiological feed adds
Add agent can stimulation of host cell and activating macrophage, improve the activity of host immune cell, promote host secrete IgA,
The antibody such as IgG, IgM, immunity of organisms and resistance are improved, while bred rapidly after entering host body, enteron aisle can be reduced
PH, itself can secreting bacteria usually suppress the growths of pathogenic bacteria, be advantageous to the growth of beneficial bacteria of intestinal tract, keep intestinal microecology
Balance and optimal immune state, improve the digestibility of nutriment.
A kind of purposes for the additive for microbe feedstuff that can strengthen fish immunity power, the additive for microbe feedstuff is added
Into fish meal, fed after well mixed, addition is the 0.05% of fish meal weight, and additive for microbe feedstuff is added to this
In feed, the mouthfeel of feed can be improved, improve absorptivity and utilization rate of the fish to nutriment in feed；Can in fish body
To produce nospecific immunity regulatory factor and antibody, the immunocompetence and resistance of body are improved；Can be bright after into body
Aobvious kill and suppression pathogenic microorganism, reduce the ill probability of fish, improve fish culture survival rate, improve meat, reduction
Cultivation input.
Embodiment 3：
A kind of additive for microbe feedstuff that can strengthen fish immunity power, composition and its parts by weight are：22 parts of the capsule of weeping forsythia, Herba Andrographitis 16
Part, 13 parts of sweet wormwood, 20 parts of rheum officinale, 1.8 parts of Radix Angelicae Sinensis, 17 parts of cordate houttuynia, 10 parts of radix glycyrrhizae, 10 parts of wilsonii, 0.7 part of garlic, dried orange peel
2.4 parts, 4.6 parts of the bark of eucommia, 1.2 parts of bacillus subtilis, 1.2 parts of Lactobacillus plantarum.
A kind of preparation method for the additive for microbe feedstuff that can strengthen fish immunity power is：The capsule of weeping forsythia is measured by formula, is worn
Heart lotus, sweet wormwood, rheum officinale, Radix Angelicae Sinensis, cordate houttuynia, radix glycyrrhizae, wilsonii, garlic, dried orange peel, the bark of eucommia, using solid-liquid ratio as 1 after crushing:5.8 plus
Water, the active peptides of powder weight 0.8% are added, be well mixed, Microwave Extraction 15min, centrifugation, produce leaching liquor；By weight
Part takes 98 parts of leaching liquor, 0.5 part of peptone, 0.3 part of beef extract, 1.3 parts of lactose, 0.3 part of sodium dihydrogen phosphate, is mixed evenly
Zymotic fluid is obtained, pH is adjusted to 7.2, sterilizing, is inoculated with formula ratio bacillus subtilis and Lactobacillus plantarum, ferment 45h at 35 DEG C, so
It is 7% to be dried to water content at 42 DEG C afterwards, produces additive for microbe feedstuff.
A kind of purposes for the additive for microbe feedstuff that can strengthen fish immunity power, the additive for microbe feedstuff is added
Into fish meal, fed after well mixed, addition is the 0.08% of fish meal weight.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Tonghua Teachers College
<120>A kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 42
<212> PRT
<213>It is artificial synthesized
<400> 1
Thr Asp His Lys Leu Cys Val Cys Val Asn Cys Phe Cys Cys Gly Ala
1 5 10 15
Ala Lys Ala Phe His Phe Tyr Lys Gly Cys Ser Leu Tyr Glu Glu Asn
20 25 30
Val Ser Tyr Lys Pro Phe Val Cys Ile Ser
35 40
Claims (10)
- A kind of 1. additive for microbe feedstuff that can strengthen fish immunity power, it is characterised in that：The composition of the microorganism formulation And its parts by weight are：Capsule of weeping forsythia 16-22 parts, Herba Andrographitis 16-19 parts, sweet wormwood 12-17 parts, rheum officinale 15-21 parts, Radix Angelicae Sinensis 1-2 parts, fish raw meat Careless 13-20 parts, radix glycyrrhizae 10-15 parts, wilsonii 7-11 parts, garlic 0.6-0.9 parts, dried orange peel 2-3 parts, bark of eucommia 3-5 parts, withered grass gemma Bar 0.8-1.3 parts, Lactobacillus plantarum 1-1.4 parts.
- A kind of 2. additive for microbe feedstuff that can strengthen fish immunity power according to claim 1, it is characterised in that：Institute The preparation method for stating additive comprises the following steps：1）The capsule of weeping forsythia, Herba Andrographitis, sweet wormwood, rheum officinale, Radix Angelicae Sinensis, cordate houttuynia, radix glycyrrhizae, wilsonii, garlic, dried orange peel, the bark of eucommia are measured by formula, Add water and active peptides after crushing, be well mixed, Microwave Extraction, centrifugation, produce leaching liquor；2）Each dispensing is measured by fermentation liquor formulation, is mixed evenly, pH is adjusted, sterilizing, is inoculated with bacillus subtilis and plant breast bar Bacterium, fermentation, produces tunning；3）Tunning is dried, produces additive for microbe feedstuff.
- 3. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：The solid-liquid ratio of powder and water is 1 in the step 1:5-6.
- 4. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：The addition of active peptides is the 0.5-0.8% of powder weight in the step 1, and its amino acid sequence is TDHKLCVCVNCFCCGAAKAFHFYKGCSLYEENVSYKPFVCIS。
- 5. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：The Microwave Extraction time is 15-25min in the step 1.
- 6. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：The composition of zymotic fluid and its parts by weight are in the step 2：Leaching liquor 95-100 parts, peptone 0.5-0.6 parts, Beef extract 0.2-0.3 parts, lactose 1.2-1.5 parts, sodium dihydrogen phosphate 0.28-0.37 parts.
- 7. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：The pH of zymotic fluid is 6.5-7.5 in the step 2.
- 8. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：Fermentation temperature is 30-35 DEG C in the step 2, pH 40-45h.
- 9. a kind of preparation method of additive for microbe feedstuff that can strengthen fish immunity power according to claim 2, its It is characterised by：Drying temperature is 30-45 DEG C in the step 3, and it is 5-8% to be dried to water content.
- A kind of 10. purposes for the additive for microbe feedstuff that can strengthen fish immunity power, it is characterised in that：The purposes is：Will Additive for microbe feedstuff is added in fish meal, is fed after well mixed, and addition is the 0.05-0.08% of fish meal weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710596355.5A CN107484919A (en) | 2017-07-20 | 2017-07-20 | A kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710596355.5A CN107484919A (en) | 2017-07-20 | 2017-07-20 | A kind of additive for microbe feedstuff that can strengthen fish immunity power and application thereof |
Publications (1)
| Publication Number | Publication Date |
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| CN107484919A true CN107484919A (en) | 2017-12-19 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247455A (en) * | 2018-08-31 | 2019-01-22 | 河南华牧生物科技有限公司 | Fresh-water fishes liver protecting Immune-enhancing effect granule and preparation method thereof |
| CN110973364A (en) * | 2019-12-24 | 2020-04-10 | 珠海市德海生物科技有限公司 | Fermented feed additive and preparation method thereof |
| CN111345399A (en) * | 2020-04-09 | 2020-06-30 | 张家口好农好牧生态养殖有限公司 | Fish feed based on chicken manure and preparation method thereof |
| CN111903874A (en) * | 2020-08-01 | 2020-11-10 | 武汉大北农水产科技有限公司 | Pelteobagrus fulvidraco feed additive, pelteobagrus fulvidraco feed and preparation method thereof |
| CN111903875A (en) * | 2020-08-01 | 2020-11-10 | 武汉大北农水产科技有限公司 | Grass carp feed additive, grass carp feed and preparation method thereof |
| CN112753849A (en) * | 2021-01-20 | 2021-05-07 | 南京众成正源生物技术有限公司 | Feed additive for improving immunity of fishes and shrimps as well as preparation method and application thereof |
| CN113396939A (en) * | 2021-06-30 | 2021-09-17 | 北京惠民发展生物科技有限公司 | Plant fermentation extract for improving immune function of aquatic animals and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1223882A (en) * | 1998-01-20 | 1999-07-28 | 曹丽文 | Immunization powder |
| CN103583810A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Fermentation type Chinese herbal medicinal immunopotentiator for erythroculter ilishaeformis |
-
2017
- 2017-07-20 CN CN201710596355.5A patent/CN107484919A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1223882A (en) * | 1998-01-20 | 1999-07-28 | 曹丽文 | Immunization powder |
| CN103583810A (en) * | 2013-11-26 | 2014-02-19 | 青岛嘉瑞生物技术有限公司 | Fermentation type Chinese herbal medicinal immunopotentiator for erythroculter ilishaeformis |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247455A (en) * | 2018-08-31 | 2019-01-22 | 河南华牧生物科技有限公司 | Fresh-water fishes liver protecting Immune-enhancing effect granule and preparation method thereof |
| CN110973364A (en) * | 2019-12-24 | 2020-04-10 | 珠海市德海生物科技有限公司 | Fermented feed additive and preparation method thereof |
| CN111345399A (en) * | 2020-04-09 | 2020-06-30 | 张家口好农好牧生态养殖有限公司 | Fish feed based on chicken manure and preparation method thereof |
| CN111903874A (en) * | 2020-08-01 | 2020-11-10 | 武汉大北农水产科技有限公司 | Pelteobagrus fulvidraco feed additive, pelteobagrus fulvidraco feed and preparation method thereof |
| CN111903875A (en) * | 2020-08-01 | 2020-11-10 | 武汉大北农水产科技有限公司 | Grass carp feed additive, grass carp feed and preparation method thereof |
| CN112753849A (en) * | 2021-01-20 | 2021-05-07 | 南京众成正源生物技术有限公司 | Feed additive for improving immunity of fishes and shrimps as well as preparation method and application thereof |
| CN113396939A (en) * | 2021-06-30 | 2021-09-17 | 北京惠民发展生物科技有限公司 | Plant fermentation extract for improving immune function of aquatic animals and preparation method thereof |
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