CN102669484A - Special animal biogen for laying hens and preparation method of special animal biogen - Google Patents
Special animal biogen for laying hens and preparation method of special animal biogen Download PDFInfo
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- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a special animal biogen for laying hens and a preparation method of the special animal biogen. The special animal biogen comprises the following ingredients: feed grade fulvic acid, dandelion general flavone and bitter soybean meal fermentation culture, wherein the bitter soybean meal fermentation culture comprises bitter soybean meal substrates and inoculation liquid, the bitter soybean meal substrates comprise bitter soybean meal, corn flour and beet molasses, the inoculation liquid comprises saccharomyces cerevisiae, lactobacillus plantarum, bacillus subtillis and bacillus licheniformis, wherein the total sum of the viable bacterium number of the saccharomyces cerevisiae, the lactobacillus plantarum, the bacillus subtillis and the bacillus licheniformis is 1*10<8>-10*10<8> cfu/g, the total sum of the viable bacterium number of the bacillus subtillis and the bacillus licheniformis is 2*10<8>-6*10<8> cfu/g, the viable bacterium number of the saccharomyces cerevisiae is 3*10<8>-7*10<8> cfu/g, and the viable bacterium number of the lactobacillus plantarum is 0.5*10<8>-2*10<8> cfu/g. The effects of promoting the growth, improving the immunity, preventing and resisting diseases and improving animal product quality are reached through restoring, regulating and exciting the digestive system and the immune system of animal bodies per se. The special animal biogen belongs to a natural green product without any hormone.
Description
Technical field
The present invention relates to a kind of animal life element and preparation method thereof, particularly relate to a kind of laying hen tailored version animal life element and preparation method thereof.
Background technology
Along with the development of fowl industry, the height of egg production is the problem that the raiser is concerned about always, directly influences raiser's economic benefit.The factor that influences the poultry laying rate is a lot, comprises feeding and management, trophic level, illumination, environmental factor, disease of finishing period etc.For improving egg output, reduce disease, general way is to add antibiotic now, but abuse of antibiotics makes that " food security " problem is serious day by day.
Since the forties in last century, antibiotic came out, antibiotic had obtained extensive use, and obvious facilitation is played in sick prevention of chicken and treatment, had brought considerable economic for numerous raisers.More antibiotic side effects also show, and affect the further raising of laying hen production performance but simultaneously.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body; Can be distributed to each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone with blood circulation; Antibiotic can cause antigenic quality to reduce; Directly influence immunologic process, thereby the inoculation of vaccine is produced harmful effect, the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can influence in the body profitable strain growth simultaneously acting on pathogen; Especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys; The harmful bacterium that hides in vivo takes advantage of the occasion to breed in a large number, and causes endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroy microecological balance, and one or more are endogenous in addition or the external source germ infects once more immediately that body causes.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most of antibiotics is excreted by ight soil, also can remain in the milk through the lactation process.After being excreted in the environment, the antibiotic of some stable in properties still can have a very long time; Thereby cause the medicament residue in the environment; These remaining medicines are slowly accumulated in human body and other plant body through livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium; Lose resistance, or body is produced toxic action because of accumulating in a large number to some disease.
Summary of the invention
It is plain and preparation method thereof to the purpose of this invention is to provide a kind of laying hen tailored version animal life, overcomes effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.
For realizing above-mentioned purpose; The invention provides a kind of laying hen tailored version animal life element and preparation method thereof, its preparation raw material comprises following constituent: feed grade fulvic acid 35%-60%, dandelion general flavone 0%-0.4%, bitter bean pulp fermentation culture 30%-65%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-15%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 1 * 10
8-10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 2 * 10
8-6 * 10
8Cfu/g, S. cervisiae 3 * 10
8-7 * 10
8Cfu/g, Lactobacillus plantarum 0.5 * 10
8-2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
It is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
The bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
The invention provides a kind of laying hen tailored version animal life element and preparation method thereof, have the following advantages:
Add Lactobacillus plantarum and can effectively available essential amino acid and various vitamin (vitamin B complex and K etc.) be provided for the host; Also can improve the biologically active of mineral element, and then be reached for that laying hen provides essential nutriment, strengthens the Nutrition and Metabolism of animal, the directly effect of short its growth.Lactobacillus plantarum is the obviously phagocytosis of activating macrophage on the one hand, on the other hand because it can be equivalent to natural active immunity in the enteron aisle field planting.They can also stimulate peritoneal macrophages, induce the generation interferon, promote cell division, produce antibody and promote cellular immunity etc., thus the non-specific and specific immune response of ability enhancing body, the resistance against diseases of raising body.The laying hen intestinal environment be slant acidity could guarantee that lactic acid bacteria can secrete organic acid by harmful bacterium invasion and attack, keep normal pH value in the enteron aisle; After adding lactic acid bacteria in addition; Can also promote gemma bacterium and saccharomycetic increment, make the beneficial bacterium status of having the advantage fast, help intestinal health.
Animal life element of the present invention is to be composited through the science compatibility to have probio, multiple Chinese herbal medicine and other nutriment that can improve functions of intestines and stomach and improve the animal immune function.Can reach growth promotion through digestive system, the immune system of repairing, regulating, exciting animal body self, improve immunity, disease resistance, improve the effect of animal product quality.These article are not for containing the natural green product of any hormone.
Through embodiment, technical scheme of the present invention is done further detailed description below.
The specific embodiment
Further specify the plain technical scheme of laying hen tailored version animal life of the present invention through specific embodiment below.
Embodiment 1
A kind of laying hen tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 40%, dandelion general flavone 0.15%, bitter bean pulp fermentation culture 59.85%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 90%, corn flour: 8.5%, beet molasses 1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 4 * 10
8Cfu/g, S. cervisiae 5 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 6: 4 (W/V).
It is brown coal, weathered coal that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal are crushed to 60 orders, and it is in 2% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 8 part by weight, in the immersion 3 hours down of 60 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1 part by weight is 30% hydrogen peroxide solution, in 80 ℃ of following activation of temperature 1.5 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 60; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain pH value=6.8 feed grade fulvic acids.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 3: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 27 hours down of 30 ℃ of temperature, anaerobic fermentation is 45 hours again.
Embodiment 2
A kind of laying hen tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 50%, dandelion general flavone 0.2%, bitter bean pulp fermentation culture 49.8%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85%, corn flour: 14%, beet molasses 1%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 9.5 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 3 * 10
8Cfu/g, S. cervisiae 6 * 10
8Cfu/g, Lactobacillus plantarum 0.5 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 5: 5 (W/V).
It is brown coal, weathered coal, peat that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat dust are broken to 80 orders, and it is in 3% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 9 part by weight, in the immersion 3 hours down of 60 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of following activation of temperature 1.8 hours, obtains activating solution;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 80; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain pH value=6.5 feed grade fulvic acids.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 4: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 34 hours down of 40 ℃ of temperature, anaerobic fermentation is 38 hours again.
Embodiment 3
A kind of laying hen tailored version animal life is plain and preparation method thereof, and its preparation raw material comprises following constituent: feed grade fulvic acid 60%, dandelion general flavone 0.3%, bitter bean pulp fermentation culture 39.7%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 95%, corn flour: 4.5%, beet molasses 0.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 2 * 10
8Cfu/g, S. cervisiae 7 * 10
8Cfu/g, Lactobacillus plantarum 1 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 7: 3 (W/V).
It is brown coal that said fulvic acid extracts raw material.
The extraction step of described feed grade fulvic acid comprises:
Brown coal powder is broken to 70 orders, and it is in 4% the sodium hydroxide solution that the raw material after pulverizing is added mass concentration by 1: 10 part by weight, in the immersion 2 hours down of 80 ℃ of temperature;
After centrifugal slagging-off, adding mass concentrations by 100: 1.5 part by weight is 30% hydrogen peroxide solution, in 75 ℃ of following activation of temperature 2 hours, obtains activating solution with said extracted liquid;
With molar concentration is that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for hydrochloric acid solution and the mass concentration of 1mol/L; Above-mentioned activating solution is mixed with the volume ratio of said compound precipitants by 1: 75; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain pH value=7 feed grade fulvic acids.
The bitter bean pulp fermentation culture of said preparation is specially: composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 5: 10 ml/kg ratio, mixes the back in the first aerobic fermentations 17 hours down of 20 ℃ of temperature, anaerobic fermentation is 55 hours again.
In the technical scheme of plain embodiment 1 to 3 embodiment of the invention described above laying hen tailored version animal life, said bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Laying hen tailored version animal life element of the present invention can be admixed the laying hen of feeding in the feed according to the ratio of 3-5 kg/ton and get final product.
Technical scheme through the plain embodiment 1 to embodiment 3 of the invention described above laying hen tailored version animal life can be found out; Compared with prior art, laying hen tailored version animal life element of the present invention has the remarkable result that improves functions of intestines and stomach, enhancing body immunity, safe ready, stable performance, is prone to preservation.Add Lactobacillus plantarum and can effectively available essential amino acid and various vitamin (vitamin B complex and K etc.) be provided for the host; Also can improve the biologically active of mineral element, and then be reached for that laying hen provides essential nutriment, strengthens the Nutrition and Metabolism of animal, the directly effect of short its growth.Lactobacillus plantarum is the obviously phagocytosis of activating macrophage on the one hand, on the other hand because it can be equivalent to natural active immunity in the enteron aisle field planting.They can also stimulate peritoneal macrophages, induce the generation interferon, promote cell division, produce antibody and promote cellular immunity etc., thus the non-specific and specific immune response of ability enhancing body, the resistance against diseases of raising body.The laying hen intestinal environment be slant acidity could guarantee that lactic acid bacteria can secrete organic acid by harmful bacterium invasion and attack, keep normal pH value in the enteron aisle; After adding lactic acid bacteria in addition; Can also promote gemma bacterium and saccharomycetic increment, make the beneficial bacterium status of having the advantage fast, help intestinal health.
Through concrete test the remarkable efficacy that laying hen tailored version animal life of the present invention is plain is described below.
Choose 2160 of the similar brown commodity eggs of 133 age in days Luo Man of body condition, be divided into 2 groups at random, be respectively control group and test group, every group of 6 repetitions, each repeats 180.Test group is added laying hen type biogen in 0.3% ratio on the basis of basal diet.Preliminary trial period 7d is just trying phase 80d.Control group and test group feeding environment, feed, way to manage all carries out according to conventional requirement of chicken house.
Table 1 laying hen type animal life element is to the influence of laying hen production performance
Notes: with line data shoulder mark different letter representation significant differences (P＜0.05), shoulder mark identical table differential different not remarkable (P＞0.05)
This test is investigated and is added the influence of animal life element to production performance of layer chicken.The result shows, compares (not adding any antibiotic) with control group, adds animal life plain (3kg/t) and can significantly improve average egg weight 3.97% (P＜0.05); Compare with control group, test group can significantly improve average day egg production 7.87% (P＜0.05); Test group is compared control group and is all had superiority at aspects such as reducing defective egg rate, death rate, average daily material consumption trend, but difference not significantly (P＞0.05); The feedstuff-egg ratio of test group is lower than control group 4.29%, significant difference (P＜0.05).
Laying hen tailored version animal life element of the present invention has the remarkable result that improves functions of intestines and stomach, enhancing body immunity, safe ready, stable performance, is prone to preservation.
It should be noted last that; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although the present invention is specified with reference to preferred embodiment; Those of ordinary skill in the art should be appreciated that and can make amendment or be equal to replacement technical scheme of the present invention, and do not break away from the spirit and the scope of technical scheme of the present invention.
Claims (4)
1. laying hen tailored version animal life is plain and preparation method thereof, and it is characterized in that: its preparation raw material comprises following constituent: feed grade fulvic acid 35%-60%, dandelion general flavone 0%-0.4%, bitter bean pulp fermentation culture 30%-65%; Described bitter bean pulp fermentation culture comprises bitter dregs of beans substrate and inoculation liquid, and wherein bitter dregs of beans substrate comprises bitter dregs of beans 85-98%, corn flour: 1-15%, beet molasses 0.5-1.5%; Inoculation liquid comprises: S. cervisiae, Lactobacillus plantarum, Bacillus subtillis and bacillus licheniformis number of viable summation 1 * 10
8-10 * 10
8Cfu/g, wherein Bacillus subtillis and bacillus licheniformis number of viable summation 2 * 10
8-6 * 10
8Cfu/g, S. cervisiae 3 * 10
8-7 * 10
8Cfu/g, Lactobacillus plantarum 0.5 * 10
8-2 * 10
8Cfu/g; The mixed proportion of bitter dregs of beans substrate and inoculation liquid is: 4-7: 3-5 (W/V).
2. laying hen tailored version animal life according to claim 1 is plain and preparation method thereof, it is characterized in that: it is one or both or three kinds in brown coal, weathered coal or the peat that said fulvic acid extracts raw material.
3. laying hen tailored version animal life according to claim 1 is plain and preparation method thereof, and it is characterized in that: the extraction step of described feed grade fulvic acid comprises:
Brown coal, weathered coal, peat or one or both or three kinds are crushed to the 60-80 order, with the raw material after pulverizing by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in immersion 2-3 hour down of 60 ℃-80 ℃ of temperature;
With said extracted liquid after centrifugal slagging-off, by 100: it is 30% hydrogen peroxide solution that the part by weight of 1-2 adds mass concentration, in 70 ℃ of-80 ℃ of following activation 1.5-2 of temperature hours, obtains activating solution;
With molar concentration be hydrochloric acid solution and the mass concentration of 1mol/L be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants; With above-mentioned activating solution and said compound precipitants by 1: the volume ratio of 50-80 mixes; Deposition is also separated and is removed palm fibre, pyrotomalenic acid; With the clarified solution concentrate drying, obtain the feed grade fulvic acid.
4. laying hen tailored version animal life according to claim 1 is plain and preparation method thereof; It is characterized in that: the bitter bean pulp fermentation culture of said preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled; Mix afterwards and descended anaerobic fermentation 38-60 hour more first aerobic fermentation 16-36 hour for 20 ℃-40 ℃ in temperature.
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