CN110269889A - Probiotics and its preparation method and application - Google Patents

Probiotics and its preparation method and application Download PDF

Info

Publication number
CN110269889A
CN110269889A CN201910563889.7A CN201910563889A CN110269889A CN 110269889 A CN110269889 A CN 110269889A CN 201910563889 A CN201910563889 A CN 201910563889A CN 110269889 A CN110269889 A CN 110269889A
Authority
CN
China
Prior art keywords
probiotics
parts
extract
extraction
ethanol solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910563889.7A
Other languages
Chinese (zh)
Inventor
于东方
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Youwei Health Technology Co Ltd
Original Assignee
Shenzhen Youwei Health Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Youwei Health Technology Co Ltd filed Critical Shenzhen Youwei Health Technology Co Ltd
Priority to CN201910563889.7A priority Critical patent/CN110269889A/en
Publication of CN110269889A publication Critical patent/CN110269889A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/733—Fructosans, e.g. inulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/736—Glucomannans or galactomannans, e.g. locust bean gum, guar gum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/738—Rosa (rose)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/10—Laxatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/12—Antidiarrhoeals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)

Abstract

The present invention relates to a kind of probiotics and its preparation method and application, which includes the component of following mass parts ratio: 2-35 parts of Fructus Rosae Normalis extract, 2-35 parts of Fructus Fici extract, 1-20 parts of compound microorganism bacterium powder, 5-45 parts of composite oligosaccharide, 20-50 parts of auxiliary protection carrier, 0.05-0.3 parts of essence.Probiotics of the invention pass through addition Fructus Rosae Normalis extract and Fructus Fici extract, it can promote the growth and breeding of probiotics, and then it is made to remain enough viable counts within the shelf-life, and it extends the shelf life, with the longer spawn activity phase, therefore whole intestines micro-ecological environment and digestion-aid effect can preferably be improved, and there is prevention and treatment intestines problem, treat diarrhea caused by intestinal bacilli illness, chronic diarrhea, the invalid diarrhea of antibiotic treatment, constipation and indigestion, abdominal distension etc., it can restore intestinal flora balance, protection liver and the removing toxic substances for enhancing liver, functions of expelling toxin, it is hypoglycemic, reducing blood lipid, enhance the multiple functions such as the disease resistances such as resistance of human body.

Description

Probiotics and its preparation method and application
Technical field
The present invention relates to medicines and health protection technical fields more particularly to a kind of probiotics and its preparation method and application.
Background technique
Microflora is huge in human gastrointestinal tract, and under normal circumstances, intestinal flora reaches dynamic equilibrium, constitutes one to people Body health has the microecosystem of great role.Unhygienic diet takes that antibiotic and pressure is big and the factors such as stress Archenteric flora will be caused to lack of proper care, lead to disease.
Probiotics are the lifes containing beneficial microbe (probiotics) and its metabolite according to microecology theories development Object preparation achievees the purpose that improve human health level by adjusting, maintaining microecological balance in enteron aisle.Probiotics packet Include three parts, i.e. probiotics, prebiotics, synbiotic.Probiotics (Probiotics) passes through improvement host intestine after referring to intake The flora ecological balance and play beneficial effect, reach the active bacteria formulation and its metabolite improved health conditions with health status. Prebiotics are to refer to selectively promote original i.e. growth of probiotics of one or more beneficial bacteria in host intestine to breed Substance, increased by the breeding of beneficial bacterium, inhibit harmful bacterial growth, to reach adjustment intestinal flora, promote body strong The purpose of health.Synbiotic refers to probiotics and prebiotics simultaneously and the preparation deposited.This based article is same with probiotics and prebiotics Shi Bingyong, enteric cavity is reached after taking can make the probiotics entered under the action of prebiotics, then row breeding increases, and be allowed to more advantageous In disease-resistant, health care the beneficial effect of performance.Such preparation appears on the market in China, and has the tendency that gradually increasing.
Numerous studies show that probiotics facilitates the treatment of many diseases, including improve diarrhea, constipation, hypoglycemic, drop blood Rouge, eczema, periodontosis, autoimmune disease etc., or increase neonatal weight, reduce kidney stone etc..As consumer health anticipates The enhancing of knowledge, the importance of microecological balance obtain unprecedented attention, and probiotics, which have become, improves human body intestines The main means of road Tiny ecosystem.Probiotics also have the advantages that other medicines are irreplaceable, i.e., " illness is cured the disease, not sick diseases prevention, The effect of disease-free health care ".It can also be taken even if Healthy People, to improve health conditions, and diarrhea patient can take, constipation Patient can also take.The consumer of the developed countries such as Japan pays much attention to for adjusting microecological balance, the market pin of product Amount is also very big.40% is all this kind of products in the specific health food (health food for being equivalent to China) of Japan, is exactly Good illustration.This kind of products are also such in the development of European Union.Chinese population inferior health in 2007 and Dysbiosis shape Condition statistical analysis explanation, 60% or more, Dysbiosis occurs the generality of China's Dysbiosis for the middle-aged and the old the most Seriously, infant takes second place, and carries out Tiny ecosystem adjusting for inferior health and is needed, and is also highly important.
Rosa roxburghii Tratt is rich in vitamin C, superoxide dismutase (superoxide dismutase, SOD), flavones and a variety of micro- Secondary element, food medicine value with higher, great exploitation potential.In recent years, there are many using Rosa roxburghii Tratt as made of primary raw material Chinese patent drug and nutrient and healthcare products ask city, such as the positive mixture of golden stichopus japonicus nine, pure plant Rosa roxburghi Juice, Ribes burejense health care tea.There is research It has been shown that, Rosa roxburghii Tratt preparation have the plurality kinds of health care medicinal functions such as anti-oxidant, antiatherosclerosis, anticancer.
Fig is the fruit of Moraceae ficus species fig, and there are cultivation in China various regions, has clearing heat and promoting fluid, and invigorating the spleen is opened Stomach, the efficacy of removing toxicity for detumescence effect.Abscess of throat, cough caused by dryness trachyphonia are cured mainly, rich in sugar, protein, amino acid, vitamin and mineral member Element etc..Contain 18 kinds of amino acid in fruit, wherein there are 8 kinds is essential amino acid.Secondly, fig have it is high medicinal It is worth, a large amount of pectin and vitamin is contained in its fruit.
With the development of microecology theory, the research and development of China's probiotics in recent years also obtains hair rapidly Exhibition.The product of the single culture of the domestic quasi- word of medicine that gone through just has LIVZON CHANGLE, life (Bifidobacterium) of rejuvenating, Infantile diarrhea (double Discrimination bacillus), cereobiogen (Bacillus cereus), Zheng Chang Sheng (bacillus licheniformis), lipid-loweringing raw (enterococcus), antibacterial life (withered grass Bacillus) etc..More bacterium combination formulations have Birid Triple Viable (Bifidobacterium, lactobacillus acidophilus, streptococcus fecalis) and Ru Kang Sheng (waxy brood cell's bar Bacterium and Lactobacillus casei) etc..There are also isomerizing lactose and mannan-oligosaccharides etc..And the preparation as health medicine and health food With regard to more.The Main way for developing new Tiny ecosystem product has been turned from simple " probiotics " or " prebiotics " in the world at present To more superior " synbiotic " this aspect of structurally reasonable, effect, i.e. " probiotics " and " prebiotics " simultaneously and deposits or is used in combination Compound micro-ecological preparation.But there is bacterial strain mostly and arrange in pairs or groups unreasonable, probiotics enteral field planting not in product currently on the market The defects of ideal, viable count are insufficient, viable bacteria is unstable, the shelf-life is short within the shelf-life.
Summary of the invention
The object of the present invention is to provide a kind of probiotics and its preparation method and application, solve in the prior art micro- The problem that ecological agent viable count is insufficient and viable bacteria is unstable, the shelf-life is short.
The technical proposal for solving the technical problem of the invention is: a kind of probiotics, including following mass fraction The component of ratio: 2-35 parts of Fructus Rosae Normalis extract, 2-35 parts of Fructus Fici extract, 1-20 parts of compound microorganism bacterium powder, composite oligosaccharide 5-45 parts, 20-50 parts of auxiliary protection carrier, 0.05-0.3 parts of essence.
In probiotics of the invention, the Fructus Rosae Normalis extract is prepared by the following method: being crushed Single Roxburgh Rose Fruit, is carried out just It walks water to extract, water filters out extracting solution after the completion of extracting；By preliminary water extract after remaining solid content take out, using ethanol solution into Row second extraction merges extracting solution twice, extracting solution is concentrated, and when solid content >=10%, is dried.
In probiotics of the invention, when carrying out preliminary water to the Single Roxburgh Rose Fruit of crushing and extracting, the Single Roxburgh Rose Fruit of crushing with The mass ratio of water is 1:1~5, and the control of water Extracting temperature, at 35 DEG C -45 DEG C, water extraction time is 90min-180min；Using When ethanol solution is to Single Roxburgh Rose Fruit second extraction is crushed, the concentration of ethanol solution is 40%~80%, the residue after preliminary water extraction The mass ratio of solid content and ethanol solution used when second extraction is 1:1~5, and is aided with ultrasound in second extraction or vibrates auxiliary Help extraction, ultrasonic power 200-400w, ultrasonic time 40-120min；Condensing mode is distillation and concentration or hyperfiltration concentration；It is dry Dry mode is spray drying, drying, freeze-drying.
In probiotics of the invention, the Fructus Fici extract is prepared by the following method: using ethanol solution pair Fig carries out refluxing extraction concentration, when solid content >=10%, is dried.
In probiotics of the invention, when extracting to fig, the concentration of ethanol solution is 5%~10%, The mass ratio of fig and ethanol solution is 1:1~15, and the control of refluxing extraction temperature is in 35 DEG C -45 DEG C, reflux extracting time 60min-180min；Drying mode is spray drying, drying, freeze-drying.
In probiotics of the invention, the compound microorganism bacterium powder includes one of Bifidobacterium or several Kind one or more of bacterial strain and lactobacillus bacterial strain, total viable count of the compound microorganism bacterium powder is 2 × 1010-1× 1013Viable count/g.
In probiotics of the invention, the composite oligosaccharide be selected from xylo-oligosaccharide, mannosan, oligofructose, At least one of galactooligosaccharide, oligoisomaltose, soyabean oligosaccharides, inulin；The auxiliary protection carrier is selected from degreasing At least one of milk powder, powdered glucose, maltodextrin, dietary fiber, calcium carbonate.
In probiotics of the invention, the form of the probiotics is pulvis, tablet or capsule form.
The present invention also provides the preparation methods of above-mentioned probiotics, comprising: in the compound microorganism bacterium powder Fructus Rosae Normalis extract, Fructus Fici extract, composite oligosaccharide is added, auxiliary protection carrier and essence are added after being sufficiently mixed, again Probiotics are made after being sufficiently mixed uniformly.
The present invention also provides above-mentioned probiotics preparation for improve gastrointestinal function, liver protecting, it is hypoglycemic, The application of the drug or health care product of reducing blood lipid and/or raising immunity of organisms.
Implement probiotics and its preparation method and application of the invention, has the advantages that of the invention micro- Ecological agent can promote the growth and breeding of probiotics by addition Fructus Rosae Normalis extract and Fructus Fici extract, so make its Enough viable counts are remained in shelf-life, and are extended the shelf life there is the longer spawn activity phase, therefore can be preferably Improve whole intestines micro-ecological environment and digestion-aid effect, and have prevention and treatment intestines problem, treats diarrhea caused by intestinal bacilli illness, slow Property diarrhea, antibiotic treatment invalid diarrhea, constipation and indigestion, abdominal distension etc., intestinal flora balance can be restored, protect liver It is dirty and enhance the removing toxic substances of liver, functions of expelling toxin, hypoglycemic, reducing blood lipid, the multiple functions such as disease resistances such as enhancing resistance of human body.
Specific embodiment
Below with reference to embodiment, probiotics and its preparation method and application of the invention are described further:
The present invention relates to a kind of probiotics, the component including following mass parts ratio: 2-35 parts of Fructus Rosae Normalis extract, nothing 2-35 parts of flowers and fruits extract, 1-20 parts of compound microorganism bacterium powder, 5-45 parts of composite oligosaccharide, 20-50 parts of auxiliary protection carrier, perfume It is 0.05-0.3 parts smart.Preferably, probiotics include the component of following mass parts ratio: 10-25 parts of Fructus Rosae Normalis extract, without flower 10-25 parts of berry extract, 5-15 parts of compound microorganism bacterium powder, 15-30 parts of composite oligosaccharide, -40 parts of auxiliary protection carrier 30 as one kind, perfume It is 0.1-0.2 parts smart.Wherein, essence preferably uses edible essence, more preferably using newborn taste essence.
Probiotics of the invention can promote the life of probiotics by addition Fructus Rosae Normalis extract and Fructus Fici extract Long breeding, and then so that it is remained enough viable counts within the shelf-life, and extend the shelf life, there is longer spawn activity Phase, therefore can preferably improve whole intestines micro-ecological environment and digestion-aid effect, and have prevention and treatment intestines problem, treat intestinal flora Diarrhea caused by lacking of proper care, chronic diarrhea, antibiotic treatment invalid diarrhea, constipation and indigestion, abdominal distension etc., can restore intestines Road colony balance protects liver and enhances the removing toxic substances of liver, functions of expelling toxin, hypoglycemic, reducing blood lipid, and enhancing resistance of human body etc. is anti- The disease multiple functions such as disease-resistant.
Wherein, Fructus Rosae Normalis extract is prepared by the following method: being crushed Single Roxburgh Rose Fruit, preliminary water extraction is carried out, after the completion of water extracts Filter out extracting solution；Remaining solid content after preliminary water is extracted takes out, and carries out second extraction using ethanol solution, merging mentions twice Liquid is taken, extracting solution is concentrated, as solid content >=10% (gross mass of extracting solution after referring to that solid content accounts for concentration, g/ It when g), is dried, dry mode can be spray drying or freeze-drying etc..Wherein, the Single Roxburgh Rose Fruit of crushing is carried out When preliminary water extracts, the Single Roxburgh Rose Fruit of crushing and the mass ratio of water are 1:1~5, and the control of water Extracting temperature is at 35 DEG C -45 DEG C, water Extraction time is 90min-180min；When using ethanol solution to Single Roxburgh Rose Fruit second extraction is crushed, the concentration of ethanol solution is The mass ratio of 40%~80% (v/v), remaining solid content and ethanol solution used when second extraction after preliminary water extraction are 1:1 ~5, and it is aided with ultrasound or vibration assisted extraction, ultrasonic power 200-400w, ultrasonic time 40-120min in second extraction.
Wherein, the mode of concentration can be hyperfiltration, and hyperfiltration refers in the application of the ethanol solution side of semi-permeable membrane The ambient pressure higher than solution osmotic pressure, only allows ethanol solution to penetrate, and other materials cannot penetrate and be trapped within film surface Process.In further embodiments, extracting solution can also be concentrated by way of distillation and concentration, or the side of filtering Formula etc..
Fructus Fici extract is prepared by the following method: carrying out refluxing extraction concentration, the mistake to fig using ethanol solution Journey can use circumfluence distillation inspissator, when solid content >=10% (total matter of extracting solution after referring to that solid content accounts for concentration Amount, g/g) when, it is dried.When extracting to fig, the concentration of ethanol solution is 5%~10% (v/v), fig with The mass ratio of ethanol solution is 1:1~15, and the control of refluxing extraction temperature is at 35 DEG C -45 DEG C, reflux extracting time 60min- 180min.Wherein, the mode of concentration can be hyperfiltration, and hyperfiltration, which refers to, applies ratio in the ethanol solution side of semi-permeable membrane The high ambient pressure of solution osmotic pressure, only allows ethanol solution to penetrate, and other materials cannot penetrate and be trapped within film surface Process.In further embodiments, extracting solution can also be concentrated by way of distillation and concentration, or the mode of filtering Deng.Dry mode can be spray drying or freeze-drying etc..
Compound microorganism bacterium powder be include in one or more of Bifidobacterium bacterial strain and lactobacillus one Kind or several bacterial strains.Compound microorganism bacterium powder is selected from bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, short bifid bar Bacterium, bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, lactobacillus bulgaricus, at least one in lactobacillus reuteri Kind.Preferably, compound microorganism bacterium powder be include Bifidobacterium, lactobacillus acidophilus, Lactobacillus casei mixture, bifid bar Bacterium, lactobacillus acidophilus, mass ratio between Lactobacillus casei mass fraction be 1-5 parts of Bifidobacterium, lactobacillus acidophilus 1-6 Part, 1-10 parts of Lactobacillus casei, it is preferable that Bifidobacterium, lactobacillus acidophilus, mass ratio between Lactobacillus casei mass parts Number is 2-3 parts of Bifidobacterium, 2-4 parts of lactobacillus acidophilus, 3-7 parts of Lactobacillus casei.
The total viable count of compound microorganism bacterium powder is 2 × 1010-1×1013CFU/g。
Above-mentioned each Bifidobacterium, lactobacillus can be directly purchase and mix, and can also carry out by the following method Preparation: bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, acidophilus cream are selected Bacillus, Lactobacillus casei, lactobacillus bulgaricus, any one or a few bacterial strain in lactobacillus reuteri, send out through single strain Ferment or mixing fermentation culture obtain zymocyte liquid；Fermentation culture medium originates pH control 4~8, cultivation temperature control 30~ 50 DEG C, incubation time is subject to fermentation termination pH 3~6；After strain fermentation, fermentation liquid is taken to carry out centrifugal concentrating, after concentration Bacterium mud is made, the emulsification protective agent that mass ratio is 5~15% (g/g) is added in bacterium mud and emulsifies, is freezed after emulsification uniformly Dry or spray drying, is made high concentration compound probiotic bacterium powder.Wherein emulsification protective agent includes but is not limited to defatted milk, albumen Peptone, amino acid, sugar etc..Culture medium includes but is not limited to MRS culture medium, LB culture medium, SOB culture medium etc., it is preferred to use MRS Culture medium, it is known in the art for being formulated, and is no longer described in detail here.
Wherein, composite oligosaccharide is selected from xylo-oligosaccharide, mannosan, oligofructose, galactooligosaccharide, oligomeric different malt At least one of sugar, soyabean oligosaccharides, inulin.
Auxiliary protection carrier in skimmed milk power, powdered glucose, maltodextrin, dietary fiber, calcium carbonate at least one Kind.
In the present invention, the form of probiotics includes but is not limited to the forms such as pulvis, tablet or capsule.
The present invention also provides the preparation methods of above-mentioned probiotics, comprising: in complex microorganism obtained above The above-mentioned Fructus Rosae Normalis extract being prepared, the above-mentioned Fructus Fici extract being prepared, above-mentioned compound oligomeric is added in bacterium powder Above-mentioned auxiliary protection carrier and essence is added in sugar after being sufficiently mixed, probiotics are made after being sufficiently mixed again uniformly.
Probiotics of the invention can promote the life of probiotics by addition Fructus Rosae Normalis extract and Fructus Fici extract Long breeding, and then so that it is remained enough viable counts within the shelf-life, and extend the shelf life, there is longer spawn activity Phase, therefore can preferably improve whole intestines micro-ecological environment and digestion-aid effect, there is prevention and treatment intestines problem, treatment intestinal flora loses Diarrhea caused by adjusting, chronic diarrhea, antibiotic treatment invalid diarrhea, constipation and indigestion, abdominal distension etc., can restore enteron aisle Colony balance improves gastrointestinal function, protects liver and enhances the removing toxic substances of liver, functions of expelling toxin, and hypoglycemic, reducing blood lipid enhances people The multiple functions such as the disease resistances such as body resistance.
It is described in detail below by specific embodiment.
(1) it prepares Rosa roxburghii Tratt berry extract: crushing Single Roxburgh Rose Fruit, carry out preliminary water extraction, the Single Roxburgh Rose Fruit of crushing and the quality of water Than being 1:2,40 DEG C of Extracting temperature, extraction time 120min filters out extracting solution；Preliminary water is mentioned remaining solid content to take out, is used The ethanol solution of 70% (v/v) concentration carries out second extraction, and it is 1 that preliminary water, which mentions remaining solid content and the mass ratio of ethanol solution: 2, it is aided with ultrasound and increases recovery rate, ultrasonic power 200-400w, ultrasonic time 100min.Merge extracting solution twice, with reverse osmosis Method extracts liquid concentration, when solid content reaches 10% or more, spray drying.
(2) it prepares Fructus Fici extract: using the second of 8% (v/v) concentration to fig using heat reflux concentration extraction device Alcoholic solution carries out refluxing extraction concentration, and the mass ratio of fig and ethanol solution is 1:4, the control of refluxing extraction temperature 35 DEG C- 45 DEG C, reflux extracting time 120min, when solid content reaches 10% or more, spray drying.
Experimental example 1
Table 1 show using above-mentioned preparation Fructus Rosae Normalis extract and the prepared probiotics of Fructus Fici extract with it is right Correlation data between ratio.Fructus Rosae Normalis extract is prepared and Fructus Fici extract is only schematic it should be noted that above-mentioned , if to Rosa roxburghii Tratt and fig extract it is extract obtained can achieve effect same, no longer enumerated.
Table 1:
Note: comparative example 1 is compared relative to embodiment 1, removes Fructus Rosae Normalis extract in comparative example 1 and fig extracts Object, other ingredients and embodiment 1 in addition to this are the components with a batch preparation；Comparative example 2 carries out pair relative to embodiment 2 Than removing Fructus Rosae Normalis extract and Fructus Fici extract in comparative example 2, other ingredients and embodiment 2 in addition to this are with a batch The component of preparation；Comparative example 3 is compared relative to embodiment 3, removes Fructus Rosae Normalis extract in comparative example 3 and fig extracts Object, other ingredients and embodiment 3 in addition to this are the components with a batch preparation.
It can be seen from 1 result of table by addition Fructus Rosae Normalis extract and Fructus Fici extract can greatly improve it is prebiotic The activity of bacterium stablizes probiotics, and then extends the validity period of probiotics.
Experimental example 2
In this experimental example, it is same that the probiotics of embodiment 1-3 are corresponding with the embodiment 1-3 in table 1 Batch, the probiotics in comparative example 1-3 are corresponding with the comparative example 1-3 in table 1 and with a batch.
Table 2:
Whether probiotics, which can colonize in vivo and play effect, is determined to the tolerance of gastric acid and cholate.According to people Physilogical characteristics, stomach is the strongest position of acidic environment in entire alimentary canal, and pH usually 3 or so, is generally by the time 1-2 hours, and the fluctuation of concentration range of cholate is 0.03-0.3% in small intestine.So using the gallbladder salinity of pH2.0 and 0.3% Simulate gastrointestinal environment.From table 2 it can be seen that probiotics show good gastrointestinal environment tolerance, viability all exists 90% or more.
It should be understood that for those of ordinary skills, it can be modified or changed according to the above description, Within all these improvement or transformation should all belong to the protection domain of appended claims of the present invention.

Claims (10)

1. a kind of probiotics, which is characterized in that the component including following mass parts ratio: 2-35 parts of Fructus Rosae Normalis extract, nothing 2-35 parts of flowers and fruits extract, 1-20 parts of compound microorganism bacterium powder, 5-45 parts of composite oligosaccharide, 20-50 parts of auxiliary protection carrier, perfume It is 0.05-0.3 parts smart.
2. probiotics according to claim 1, which is characterized in that the Fructus Rosae Normalis extract is prepared by the following method: Single Roxburgh Rose Fruit is crushed, preliminary water extraction is carried out, water filters out extracting solution after the completion of extracting；Remaining solid content after preliminary water is extracted takes Out, second extraction is carried out using ethanol solution, merges extracting solution twice, extracting solution is concentrated, when solid content >= When 10%, it is dried.
3. probiotics according to claim 2, which is characterized in that carry out preliminary water extraction to the Single Roxburgh Rose Fruit of crushing When, the Single Roxburgh Rose Fruit of crushing and the mass ratio of water are 1:1~5, and the control of water Extracting temperature is at 35 DEG C -45 DEG C, and water extraction time is 90min-180min；When using ethanol solution to Single Roxburgh Rose Fruit second extraction is crushed, the concentration of ethanol solution is 40%~80%, just The mass ratio for walking the remaining solid content after water extracts and ethanol solution used when second extraction is 1:1~5, and in second extraction It is aided with ultrasound or vibration assisted extraction, ultrasonic power 200-400w, ultrasonic time 40-120min；Condensing mode is distillation and concentration Or hyperfiltration concentration；Drying mode is spray drying, drying, freeze-drying.
4. probiotics according to claim 1, which is characterized in that the Fructus Fici extract is made by the following method It is standby: refluxing extraction concentration being carried out to fig using ethanol solution, when solid content >=10%, is dried.
5. probiotics according to claim 4, which is characterized in that when being extracted to fig, ethanol solution Concentration is 5%~10%, and the mass ratio of fig and ethanol solution is 1:1~15, and refluxing extraction temperature is controlled at 35 DEG C -45 DEG C, reflux extracting time 60min-180min；Drying mode is spray drying, drying, freeze-drying.
6. probiotics according to claim 1, which is characterized in that the compound microorganism bacterium powder includes bifid bar One or more of one or more of Pseudomonas bacterial strain and lactobacillus bacterial strain, total work of the compound microorganism bacterium powder Bacterium number is 2 × 1010-1×1013Viable count/g.
7. probiotics according to claim 1, which is characterized in that the composite oligosaccharide is selected from xylo-oligosaccharide, sweet Reveal at least one of glycan, oligofructose, galactooligosaccharide, oligoisomaltose, soyabean oligosaccharides, inulin；The auxiliary Carrier is protected to be selected from least one of skimmed milk power, powdered glucose, maltodextrin, dietary fiber, calcium carbonate.
8. probiotics according to claim 1, which is characterized in that the form of the probiotics is pulvis, piece Agent or capsule form.
9. a kind of preparation method of any probiotics of claim 1-8 characterized by comprising described multiple It closes and Fructus Rosae Normalis extract, Fructus Fici extract, composite oligosaccharide is added in microbial germ powder, auxiliary protection is added after being sufficiently mixed and carries Probiotics are made after being sufficiently mixed again uniformly in body and essence.
10. any probiotics of claim 1-8 preparation for improve gastrointestinal function, liver protecting, it is hypoglycemic, The application of the drug or health care product of reducing blood lipid and/or raising immunity of organisms.
CN201910563889.7A 2019-06-26 2019-06-26 Probiotics and its preparation method and application Pending CN110269889A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910563889.7A CN110269889A (en) 2019-06-26 2019-06-26 Probiotics and its preparation method and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910563889.7A CN110269889A (en) 2019-06-26 2019-06-26 Probiotics and its preparation method and application

Publications (1)

Publication Number Publication Date
CN110269889A true CN110269889A (en) 2019-09-24

Family

ID=67963471

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910563889.7A Pending CN110269889A (en) 2019-06-26 2019-06-26 Probiotics and its preparation method and application

Country Status (1)

Country Link
CN (1) CN110269889A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113729183A (en) * 2020-10-09 2021-12-03 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) Method for preparing roxburgh rose health product with intestinal function improving function
CN114190546A (en) * 2021-11-24 2022-03-18 重庆万源佳药业有限公司 Microecological extract capable of improving immunity and preparation method thereof
CN114680308A (en) * 2020-12-31 2022-07-01 贵州乾之象生物科技有限责任公司 Freeze-dried roxburgh rose composite capsule and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496822A (en) * 2009-02-18 2009-08-05 上海谱莱生物技术有限公司 Composite probiotics micro-ecological formulation and preparation method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101496822A (en) * 2009-02-18 2009-08-05 上海谱莱生物技术有限公司 Composite probiotics micro-ecological formulation and preparation method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周彩莲: "《养花，赏花，用花：旺家健康花草养护图鉴》", 30 September 2016, 中国轻工业出版社 *
孟掉琴等: "混菌发酵苹果浊汁的益生菌筛选及其发酵动力学模型构建", 《食品科学》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113729183A (en) * 2020-10-09 2021-12-03 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) Method for preparing roxburgh rose health product with intestinal function improving function
CN114680308A (en) * 2020-12-31 2022-07-01 贵州乾之象生物科技有限责任公司 Freeze-dried roxburgh rose composite capsule and preparation method thereof
CN114190546A (en) * 2021-11-24 2022-03-18 重庆万源佳药业有限公司 Microecological extract capable of improving immunity and preparation method thereof

Similar Documents

Publication Publication Date Title
CN105146614B (en) A kind of functional calcium fruit ferment, enzyme beverage and its production method
US9649347B2 (en) Protective effects and application of a Lactobacillus rhamnosus on the alleviation of chronic alcoholic liver injury
US20140363501A1 (en) The protective effects and application of a Lactobacillus rhamnosus on the alleviation of chronic alcoholic liver injury
JP5340302B2 (en) A composition for relieving hangover, containing Kamisuwato containing Kigushi as an active ingredient
CN109123295A (en) A kind of probiotics solid beverage and preparation method thereof
KR20170038462A (en) Composition comprising lactic acid bacteria derived extracellular vesicles for preventing hair loss or promoting hair growth
CN101257910A (en) Cholesterol absorption inhibitor
CN106135977A (en) There is Cerasus humilis Bunge ferment, enzyme beverage and the production method thereof of improving eyesight function of replenishing the calcium
CN107048360A (en) Prepare the fermentation composition and preparation method of the plant enzyme with recuperating gastrointestinal tract function of relaxing bowel
CN110269889A (en) Probiotics and its preparation method and application
CN102935092A (en) Novel lactobacilli and composition thereof, and application thereof in preparing medicines used for ameliorating diabetes and complications thereof
CN102274245A (en) Novel lactacidophilus, composition thereof and use thereof in preparation of medicines for relieving diabetes mellitus and complications
CN105641209A (en) Probiotics composition with function of improving immunity and preparing method and application of probiotics composition
CN105707805A (en) Honey cream and preparation method thereof
CN106551378A (en) A kind of compositionss of probiotics fermention Cordyceps militaris (L.) Link. and its preparation method and application
CN106035806A (en) Tea powder composition, and preparation method and application thereof
CN109315769A (en) It is a kind of for improving the composition and preparation method thereof of human body enteral environment
CN105707625A (en) Composition obtained by fermenting folium eriobotryae with probiotics and preparing method and application of composition
CN105831666A (en) Probiotic honey composition and preparation method thereof
CN105685913A (en) Honey product and preparation method thereof
KR101750522B1 (en) Essence cosmetic composition containing velvet-extract
CN109045151A (en) A kind of composition and preparation method thereof of regulation intestinal flora
CN104771416A (en) Lactobacillus reuteri GMNL-263 composition for control weight, and uses thereof
CN106173728A (en) A kind of enzyme beverage with Fructus Cucurbitae moschatae as main material and preparation method thereof
CN105815726A (en) Honey product for improving immunity and making method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination