CN105641209A - Probiotics composition with function of improving immunity and preparing method and application of probiotics composition - Google Patents
Probiotics composition with function of improving immunity and preparing method and application of probiotics composition Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
Abstract
The invention discloses a probiotics composition with the function of improving immunity and a preparing method and application of the probiotics composition. The probiotics composition is prepared from, by mass, 60-70% of dry probiotics powder, 20-30% of radix astragali extract powder and 10-20% of fructus lycii extract powder. Probiotics and traditional Chinese medicine are two internationally-recognized prebiotics substances, are interlinked in action mechanism and can perfectly complement each other in the specific using process to jointly bring the advantages to full play. According to the probiotics composition, probiotics and traditional Chinese medicine are perfectly combined, multiplication of probiotics is promoted through the probiotics increasing function of traditional Chinese medicine, the microecological balance in the intestines is regulated, and thus the effect of improving immunity is achieved.
Description
[technical field]
The invention belongs to biomedicine field, be specifically related to a kind of probiotic composition having and improving immunity function and preparation method thereof and application.
[background technology]
Immunity is a kind of active defense mechanism of human body self, and maintenance physiology and vital movement tool are of great significance. Maintain the immunocyte of immunologic function in human body and have T cell, B cell, neutrophils, macrophage, immunity is not enough or immunity is too high, capital is to self bringing adverse effect, and main manifestations is inappetence, malnutrition, absentminded, have a delicate constitution, feel run-down, sleep disorder etc. Modern society is along with the raising of health care consciousness, and preventing and treating " not sick " is also had requirements at the higher level by people. But owing to modern society's rhythm is fast, people can not accomplish enhancing immunity of taking regular exercise every day mostly. Therefore, the method seeking energy enhancing immunity safer, more effective, the health of the mankind is just particularly important.
[summary of the invention]
The purpose of the present invention it is an object of the invention to provide a kind of probiotic composition having and improving immunity function, and said composition can be effectively improved immunity of organisms, promotes health.
Another object of the present invention is to the preparation method that the above-mentioned probiotic composition having and improving immunity function is provided.
It is still another object of the present invention to provide the application of the above-mentioned probiotic composition having and improving immunity function.
For achieving the above object, provided by the invention have the probiotic composition improving immunity function, comprises following component by mass percentage: probiotic bacteria dry powder 60��70%, Radix Astragali extract powder 20��30%, wolfberry fruit extract powder 10��20%.
Preferably, the described probiotic composition improving immunity function that has comprises following component by mass percentage: probiotic bacteria dry powder 70%, Radix Astragali extract powder 20%, wolfberry fruit extract powder 10%.
Further, described probiotic bacteria dry powder is formed in the ratio of 1:1 by by bifidobacteria infantis, lactobacillus two kinds.
The present invention also provides for a kind of preparation method with the probiotic composition improving immunity function, and it comprises the steps of:
(1) step of selecting materials is: choose containing bifidobacteria infantis, bacillus acidophilus, Bacillus coagulans probiotic bacteria dry powder, high-quality Radix Astragali extract powder, wolfberry fruit extract powder;
(2) sifting step is: the probiotic bacteria dry powder chosen, Radix Astragali extract powder, wolfberry fruit extract powder are crossed 80��100 mesh sieves respectively;
(3) batching step is: weigh standby by the probiotic bacteria dry powder after sieving, Radix Astragali extract powder, wolfberry fruit extract powder; Described each constituent mass percentage ratio is probiotic bacteria dry powder 60��70%, Radix Astragali extract powder 20��30%, wolfberry fruit extract powder 10��20%;
(4) blend step is: mixed by the raw material prepared to uniform color consistent in three-dimensional mixer, and namely no color differnece obtains having the probiotic composition improving immunity function.
Described probiotic bacteria dry powder can pass through to buy the raw material of listing or grope the mode of preparation technology voluntarily to obtain. The preparation technology of selected probiotic bacteria dry powder is optimized by the present invention; Described probiotic bacteria process for preparation of dry powder step is as follows:
1) respectively by bifidobacteria infantis, lactobacillus through culture medium activate after, be inoculated in fermentation medium, shaken cultivation, make cell concentration reach exponential phase 0.5��1.0 �� 109Cfu/ml;
2) by step 1) in centrifugal after the mixing of each strain fermentating liquid, abandon supernatant, bacterial sediment washs, centrifugal, and sterilizing obtains bacterium mud;
3) preparing dry protective agent; The protectant formula of described dry powder consists of: cycloheptaamylose 10g, lactose 2-5g, trehalose 0.5-2g, yeast extract 0.5-2g, vitamin E 0.05-0.1g, distilled water 100mL;
4) dry powder protective agent and bacterium mud are mixed, carry out film formation reaction, then, by mixture pre-freeze, then lyophilization, make the active probiotic dry powder with encapsulating layer.
The present invention also provides for a kind of probiotic composition with raising immunity function and improves the application in immunity food/health product in preparation.
Preferably, there is described in the probiotic composition improving immunity function and be used in powder, tablet, capsule, granule, electuary, fruit jelly, beverage, milk product, Yoghourt or biscuit baked goods; Described health product are any one in powder, tablet, capsule, liquid agent, suspension, Emulsion, oral liquid, fruit jelly or electuary.
Above-mentioned have the probiotic composition improving immunity function application in preparation raising immunity food/health product, and said composition can add one or more pharmaceutically acceptable carriers and make the dosage forms such as tablet, capsule, granule, oral liquid.
The present invention also provides for a kind of raising immunity food/health product, and it comprises the above-mentioned probiotic composition having and improving immunity function.
The Radix Astragali can strengthen and regulate body's immunity, and interferon system is had facilitation, can improve the premunition of body; The multiple viral institute such as infected by influenza cytopathogenic effect has slight inhibitory action, and influenza virus infected has protective effect; There is wide antibacterial action. Fructus Lycii contains 18 seed amino acids, and wherein 8 seed amino acids are essential amino acids. Mineral is except containing calcium, and phosphorus, outside ferrum etc., possibly together with a number of organic germanium. Containing vitamin also relatively comprehensively with abundant, including vitamin B1, vitamin B2, C, niconacid and carotene etc., there is enhancing immunologic function, health is extremely beneficial. Both of which has that toxic and side effects is little, material is readily available and can the advantage such as long-term taking.
Promote probiotic bacteria propagation in intestinal, suppress harmful bacteria growth simultaneously, then play the multiple important physiological action of body, people oneself have personal cognition.Particularly the enhancing immunity effect of probiotic bacteria is own is extensively admitted: the surface area of normal person's gastrointestinal mucosal has 300m2, this area with 3/4 basketball court is suitable, the digestive tract immune organ that to be changed in human body because relevant lymphoid tissue (GALT) acts on maximum. Human gastrointestinal tract holds about 1014Individual microorganism, the cell of human body self has 1013Individual, account for the 10% of microbial cell. L0 is there is in every meter of small intestinal10The individual cell producing immunoglobulin, accounts for the 80% of all generation immune globulin leukocyte of human body. Thus the immunocyte of digestive tract different parts can excite panimmunity to react, part lactobacillus and bifidobacterium bacteria are being considered as immunostimulant or " biological response modifier " in varying degrees. Therefore, probiotics preparation as a kind of new method improving immunity, and can have the advantages such as safe source, effect be notable, without any side effects.
The use of Chinese medicine traditionally, the overwhelming majority is played a role by oral absorption with the form of prescription, effective ingredient in Chinese medicine inevitably associates with intestinal microbial population after entering intestinal, some composition is absorbed after the effect generation bioconversion of corresponding antibacterial, therefore, in internal milieu, intestinal microbial population has been one of key factor of Chinese Medicine Effective Component Biotransformation. Probiotics and Chinese medicine are the two internationally recognized prebiotic substances of class, and both are very much like in basic theories viewpoint, be intimately tied up with; The mechanism of action shows great uniformity; Also can be perfect complementary in specifically used process, jointly make the most of the advantage. Thus Chinese medicine is for probiotics, it it is excellent prebiotics. The present invention, by probiotic bacteria and Chinese medicine perfect adaptation, have found and has the probiotic combinations composition formula significantly improving immunity effect.
The beneficial effects of the present invention is: the probiotic composition of the present invention directly feeds intake with probiotic bacteria dry powder, Radix Astragali extract powder, wolfberry fruit extract powder for raw material, and scientific matching forms by weight. It contains vitamin K, the nutritional labelings such as vitamin B12, pyridoxol, biotin, folic acid, nicotinic acid and thiamine, preparation technology is simple, the drug loss that causes when avoiding extraction and the bigger shortcoming of the different product mass discrepancy caused due to crude drug mass discrepancy, making product purity higher, impurity is few, and safety is higher, and mass discrepancy is little between different batches, quality is evenly stable. By Chinese medicine and probiotic bacteria reasonable compatibility medication, proving through pharmaceutical test, drug effect is better than being used alone any raw material, and each effective ingredient is synergism, has raising immunity, promotes the effects such as body health.
[detailed description of the invention]
Below in conjunction with embodiment, the present invention done further detailed description, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) select materials
Prepare probiotic bacteria dry powder:
1) respectively by bifidobacteria infantis, lactobacillus through culture medium activate after, be inoculated in fermentation medium, shaken cultivation, make cell concentration reach exponential phase 0.5��1.0 �� 109Cfu/ml;
The preparation of bacillus bifidus seed liquid: take out culture presevation pipe, draw flat board with bacillus bifidus solid medium and recover, 37 degree of Anaerobic culturel 72 hours. Under flat board in 50 milliliters of Medium of Bifidobacteriums of the single colony inoculation of picking, 37 degree of Anaerobic culturel 48 hours in incubator. Seed adopts the inoculum concentration of 5%, is seeded in the big triangular flask of 10L (liquid amount is 9.5L), 37 degree of Anaerobic culturel 20-28 hour, detects its bacterial concentration, and bacterial content, more than 1,000,000,000/milliliter, can be used as bacillus bifidus liquid-spawn inoculation.Wherein said Medium of Bifidobacterium: peptone 5.0g/L, yeast extract 5g/L, Carnis Bovis seu Bubali cream 5.0g/L, tryptone 10.0g/L, glucose 10.0g/L, sodium acetate 5.0g/L, dibasic ammonium citrate 2.0g/L, tween 80 1.0ml/L, magnesium sulfate 0.1g/L, zinc sulfate 0.25g/L, dipotassium hydrogen phosphate 2g/L, add agar 2% in solid medium. The condition of medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.
The preparation of lactobacillus seed liquor: take out culture presevation pipe, draw flat board with MRS solid medium and recover, 37 degree of Anaerobic culturel 48 hours. Under flat board in 250 milliliters of MRS culture medium of the single colony inoculation of picking, 37 degree of Anaerobic culturel 24 hours in incubator. Seed adopts the inoculum concentration of 5%, is seeded in the big triangular flask of 10L (liquid amount is 9L), 37 degree of Anaerobic culturel 20-28 hour, detects its bacterial concentration, and bacterial content, more than 10,000,000,000/milliliter, can be used as lactobacillus liquid-spawn inoculation. Wherein said MRS culture medium: peptone 10g/L, yeast extract 5g/L, Carnis Bovis seu Bubali cream 10g/L, glucose 20g/L, sodium acetate 5g/L, dibasic ammonium citrate 2g/L, tween 80 1ml/L, magnesium sulfate 0.6g/L, manganese sulfate 0.05g/L, dipotassium hydrogen phosphate 2g/L, agar 20g/L, calcium carbonate 20g/L, adds agar 2% in solid medium. Wherein said lactic acid bacteria seed culture medium: glucose 5%, peptone 1.5%, Carnis Bovis seu Bubali cream 0.75%, sodium chloride 0.1%, manganese sulfate 0.001%, magnesium sulfate 0.02%, sodium acetate 0.05%, calcium carbonate 1%, pH6.0. The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.
Radix Astragali extract powder is the root extract of leguminous plant Radix Astagali. Perennial herb is high 50-100 centimetre. Main root is plump, wooden, normal branch, canescence. Stem is upright, and top multi-branched has thin rib, by white pubescence. Perennial herb is high 50-100 centimetre. Originate in the ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang. The history of medicinal existing more than 2000 year so far of the Radix Astragali, has enhancing immunity, strengthens energy, resisting fatigue, mutation, protect the liver, it is suppressed that the effect of osteoclast. Astragalus polysaccharides has blood fat reducing, namely reduces the effect of cholesterol and triglyceride, high density lipoprotein increasing; Can prevent and treat cardiovascular and cerebrovascular disease, such as atherosclerosis, coronary artery pathological changes, peripheral angiopathy and hyperlipemia etc. Separately there are some researches show, bacillus bifidus is had and significantly promotes growth by the Radix Astragali of 12.5%.
Wolfberry fruit extract powder is be the Lycium barbarum fruits extract of Solanaceae Lycium. Multi-branched shrub plant, all has distribution both at home and abroad. Fructus Lycii whole body is all precious, and Fructus Lycii is rich in multiple nutritional components such as Fructus Lycii proteoglycan, vitamin C, phosphorus, ferrum. In Fructus Lycii peel rich in one of effective ingredient lycium barbarum polysaccharide (LBP) hemopoietic function of bone marrow and every Immunological Markers for Cell-Mediated Immunity are had obvious potentiation, the active quantities of the superoxide dismutase (SOD) of body blood, liver and muscular tissue can be significantly improved, thus being conducive to the removing of active oxygen, slow down aging and resisting fatigue; Increase the quantity of antibody forming cell, improve antibody effects.
The Radix Astragali extract powder, the wolfberry fruit extract powder that use in the present invention are raw material of Chinese medicine product after water carries, it is commercially extensive use all, various extracts be prepared by existing technology, the product obtained is difference to some extent in material concentration simply, and its various products all can practice in the present invention.
2) being centrifuged after being mixed by each strain fermentating liquid, abandon supernatant, bacterial sediment washs, centrifugal, and sterilizing obtains bacterium mud;
3) preparing dry protective agent; The protectant formula of dry powder consists of: cycloheptaamylose 10g, lactose 2-5g, trehalose 0.5-2g, yeast extract 0.5-2g, vitamin E 0.05-0.1g, distilled water 100mL;
4) dry powder protective agent and bacterium mud are mixed, carry out film formation reaction, then, by mixture pre-freeze, then lyophilization, make the active probiotic dry powder with encapsulating layer;
Above-mentioned lactobacillus is at least two equal proportion mixed vaccine in Lactobacillus bulgaricus, Lactobacillus delbrueckii subsp. lactis and bacillus acidophilus, it is preferable that Lactobacillus bulgaricus, two kinds of equal proportion mixed vaccine of bacillus acidophilus.
(2) sieve
Probiotic bacteria dry powder in step (1), Radix Astragali extract powder, wolfberry fruit extract powder are crossed respectively 80��100 mesh sieves.
(3) dispensing
Weighing the raw material after sieving, by probiotic bacteria dry powder 70%, Radix Astragali extract powder 20%, wolfberry fruit extract powder 10% dispensing, above content is weight percent content.
(4) mixing
The probiotic bacteria dry powder prepared, Radix Astragali extract powder, wolfberry fruit extract powder are mixed to uniform color consistent in three-dimensional mixer, no color differnece, namely obtain that there is the probiotic composition improving immunity function.
Embodiment 2
Concrete steps, with embodiment 1, are different in that: dispensing in step (3): weighing the raw material after sieving, by probiotic bacteria dry powder 60%, Radix Astragali extract powder 30%, wolfberry fruit extract powder 10% dispensing, above content is weight percent content.
Embodiment 3
Concrete steps, with embodiment 1, are different in that: dispensing in step (3): weighing the raw material after sieving, by probiotic bacteria dry powder 60%, Radix Astragali extract powder 20%, wolfberry fruit extract powder 20% dispensing, above content is weight percent content.
Embodiment 4
Concrete steps, with embodiment 1, are different in that: dispensing in step (3): weighing the raw material after sieving, by probiotic bacteria dry powder 65%, Radix Astragali extract powder 25%, wolfberry fruit extract powder 10% dispensing, above content is weight percent content.
Embodiment 5
Concrete steps, with embodiment 1, are different in that: dispensing in step (3): weighing the raw material after sieving, by probiotic bacteria dry powder 65%, Radix Astragali extract powder 20%, wolfberry fruit extract powder 15% dispensing, above content is weight percent content.
Experimental example 1 has the impact of the mouse spleen lymphocyte conversion reaction that ConA is induced by the probiotic composition improving immunity function
(1) laboratory animal
Select the SPF level Kunming female white mice of kind 48,6-8 week old (body weight 18��22g), raise at Wuhan University's Life Science College Experimental Animal Center receptacle.
(2) dosage and packet
Test by human body recommended amounts every day (in child body weight 30kg, recommended amounts is 0.05g/kg BW) 5 times (0.25g/kg BW), 10 times (0.5g/kg BW) and 30 times (1.5g/kg BW) be divided into matched group and basic, normal, high dosage group totally four groups, often group 12.
(3) method of feeding is filled
Experiment time-division another name take sample 0.50,1.00,3.00g, be configured to the sample liquid of 20mL with pure water, respectively for basic, normal, high three dosage treated animal gavages. After animal adapts to three days in laboratory conditions, at random 48 Mus being divided into four groups, often group 12, every day, gavage gave embodiment 1 compositions, adjusted according to the increase and decrease of body weight weekly to sample amount, continuous gavage surrounding. Matched group gavages sterile purified water.
(4) the mouse spleen lymphocyte conversion test (mtt assay) of ConA induction
Lymphproliferation response: cell suspension point holes added in 24 well culture plates, every hole 1mL, a hole 75 �� LConA liquid (being equivalent to 7.5 �� g/mL), 5%CO, as comparison, is put in another hole2, cultivate 72h for 37 DEG C.Cultivation terminates first 4 hours, and every hole sucks supernatant 0.7mL, adds 0.7mL serum-free RPMI1640 culture fluid, is simultaneously introduced MTT (5mg/mL) 50 �� L/ hole, continues to cultivate 4h. After cultivation terminates, every hole adds 1mL isopropanol, piping and druming mixing, makes purple crystal dissolve, divides and be added in 96 well culture plates, make the Duplicate Samples in 3 holes, measures optical density value with 570nm wavelength.
(5) experimental result
Mouse spleen lymphocyte conversion reaction to ConA induction, its high dose group optical density difference is higher than matched group, and difference has significant (see table 1). Showing that the Splenic vein hemodynamics result of the test of probiotic electuary is for positive, given the test agent has enhancing cellular immune function effect.
The impact (x �� s) on mouse spleen lymphocyte conversion reaction of table 1 embodiment 1 compositions
| Group | Dosage (g/kg BW) | Number of animals (only) | Optical density difference | P value (with matched group ratio) |
| Matched group | 0.00 | 12 | 0.47��0.05 | |
| Low dosage | 0.25 | 12 | 0.49��0.05 | >0.05 |
| Middle dosage | 0.50 | 12 | 0.51��0.06 | >0.05 |
| High dose | 1.50 | 12 | 0.53��0.04 | <0.05 |
Experimental example 2 has the probiotic composition the improving immunity function impact on animal subject NK cytoactive
(1) laboratory animal
Select the SPF level Kunming female white mice of kind 48,6-8 week old (body weight 18��22g), raise at Wuhan University's Life Science College Experimental Animal Center receptacle.
(2) dosage and packet
Test by human body recommended amounts every day (in child body weight 30kg, recommended amounts is 0.05g/kg BW) 5 times (0.25g/kg BW), 10 times (0.5g/kg BW) and 30 times (1.5g/kg BW) be divided into matched group and basic, normal, high dosage group totally four groups, often group 12.
(3) method of feeding is filled
Experiment time-division another name take sample 0.50,1.00,3.00g, be configured to the sample liquid of 20mL with pure water, respectively for basic, normal, high three dosage treated animal gavages. After animal adapts to three days in laboratory conditions, at random 48 Mus being divided into four groups, often group 12, every day, gavage gave embodiment 1 compositions, adjusted according to the increase and decrease of body weight weekly to sample amount, continuous gavage surrounding. Matched group gavages sterile purified water.
(4) NK cytoactive detection (LDH method)
NK cytoactive detects: taking concentration is 4*105The YAC-1 target cell of individual/mL and each 100 �� L of effector lymphocyte (effect target is than 50:1), add U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and each 100 �� L of culture fluid, and the maximum release aperture of target cell adds target cell and 1%NP40Each 100 �� L; Above-mentioned every it is all provided with three multiple holes, in 37 DEG C, 5%CO2Cultivating 4 hours in incubator, be then centrifuged 5 minutes with 1500rpm by 96 well culture plates, every hole is drawn in 96 well culture plates at the bottom of supernatant 100 �� L horizontalization, it is simultaneously introduced LDH matrix liquid 100 �� L, reacting 3 minutes, every hole adds lmol/LHcl30 �� L, joins instrument 492nm place at enzyme and measures optical density value.
(5) experimental result
The NK cytoactive of middle and high dosage group is higher than matched group, and difference all has significant (see table 2). Showing that animal subject NK cell activity assays result is the positive by probiotic electuary, given the test agent has enhancing NK cytoactive effect.
The impact (n=11, x �� s) on NK cells in mice activity of table 2 embodiment 1 compositions
NK cytoactive conversion value: *=Sin-1(P)l/2
Above-described embodiment is the present invention preferably embodiment; but embodiments of the present invention are also not restricted to the described embodiments; the change made under other any spirit without departing from the present invention and principle, modification, replacement, combination, simplification; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. one kind has the probiotic composition improving immunity function, it is characterised in that: it comprises following component by mass percentage: probiotic bacteria dry powder 60��70%, Radix Astragali extract powder 20��30%, wolfberry fruit extract powder 10��20%.
2. according to claim 1 have the probiotic composition improving immunity function, it is characterised in that: it comprises following component by mass percentage: probiotic bacteria dry powder 70%, Radix Astragali extract powder 20%, wolfberry fruit extract powder 10%.
3. according to claim 1 and 2 have the probiotic composition improving immunity function, it is characterised in that: the ratio that described probiotic bacteria dry powder is 1:1 in mass ratio by bifidobacteria infantis, lactobacillus forms.
4. prepare the probiotic composition as claimed in claim 1 with raising immunity function for one kind, it is characterised in that: it comprises the steps of:
(1) select materials: choose the probiotic bacteria dry powder containing bifidobacteria infantis, lactobacillus, high-quality Radix Astragali extract powder, wolfberry fruit extract powder;
(2) sieve: the probiotic bacteria dry powder chosen, Radix Astragali extract powder, wolfberry fruit extract powder are crossed respectively 80��100 mesh sieves;
(3) dispensing: the probiotic bacteria dry powder after sieving, Radix Astragali extract powder, wolfberry fruit extract powder are weighed standby; Described each constituent mass percentage ratio is probiotic bacteria dry powder 60��70%, Radix Astragali extract powder 20��30%, wolfberry fruit extract powder 10��20%;
(4) mixing: the raw material prepared is mixed in three-dimensional mixer to uniform color consistent, no color differnece, namely obtain that there is the probiotic composition improving immunity function.
5. the preparation method according to claim 4 with the probiotic composition improving immunity function, it is characterised in that: described probiotic bacteria process for preparation of dry powder step is as follows:
1) respectively by bifidobacteria infantis, lactobacillus through culture medium activate after, be inoculated in fermentation medium, shaken cultivation, make cell concentration reach exponential phase 0.5��1.0 �� 109Cfu/ml;
2) by step 1) in centrifugal after the mixing of each strain fermentating liquid, abandon supernatant, bacterial sediment washs, centrifugal, and sterilizing obtains bacterium mud;
3) preparing dry protective agent; The protectant formula of described dry powder consists of: cycloheptaamylose 10g, lactose 2-5g, trehalose 0.5-2g, yeast extract 0.5-2g, vitamin E 0.05-0.1g, distilled water 100mL;
4) dry powder protective agent and bacterium mud are mixed, carry out film formation reaction, then, by mixture pre-freeze, then lyophilization, make the active probiotic dry powder with encapsulating layer.
6. one kind has the probiotic composition improving immunity function application in preparation raising immunity food/health product as claimed in claim 1 or 2.
7. according to claim 6 have the application that the probiotic composition improving immunity function improves in immunity food/health product in preparation, it is characterised in that: described in there is the probiotic composition improving immunity function be used in powder, tablet, capsule, granule, electuary, fruit jelly, beverage, milk product, Yoghourt or biscuit baked goods; Described health product are any one in powder, tablet, capsule, liquid agent, suspension, Emulsion, oral liquid, fruit jelly or electuary.
8. one kind is improved immunity food/health product, it is characterised in that: it comprises the probiotic composition as claimed in claim 1 or 2 with raising immunity function.
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| CN107198231A (en) * | 2017-06-20 | 2017-09-26 | 山东瑞芝生物科技有限公司 | A kind of probiotic health food formula and preparation method thereof |
| CN108310238A (en) * | 2018-03-15 | 2018-07-24 | 武汉虹辰远荣生物制品有限公司 | A kind of probiotic composition of prevention diarrhea |
| CN109078190A (en) * | 2018-10-30 | 2018-12-25 | 单风平 | A kind of composition and preparation method for cancer patient's immunity of organism balance adjustment |
| CN109123671A (en) * | 2018-08-29 | 2019-01-04 | 安徽谷益生物科技有限公司 | A kind of functional food preventing cold in children and strengthen immunity |
| CN109846044A (en) * | 2019-01-31 | 2019-06-07 | 山东环亿生物科技有限公司 | With improvement memory, the probiotic composition for improving immunity and preparation method thereof |
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| CN106262118A (en) * | 2016-08-30 | 2017-01-04 | 宁夏沃福百瑞枸杞产业股份有限公司 | The preparation method of probiotic bacteria Fructus Lycii sheet |
| CN107198231A (en) * | 2017-06-20 | 2017-09-26 | 山东瑞芝生物科技有限公司 | A kind of probiotic health food formula and preparation method thereof |
| CN108310238A (en) * | 2018-03-15 | 2018-07-24 | 武汉虹辰远荣生物制品有限公司 | A kind of probiotic composition of prevention diarrhea |
| CN109123671A (en) * | 2018-08-29 | 2019-01-04 | 安徽谷益生物科技有限公司 | A kind of functional food preventing cold in children and strengthen immunity |
| CN109078190A (en) * | 2018-10-30 | 2018-12-25 | 单风平 | A kind of composition and preparation method for cancer patient's immunity of organism balance adjustment |
| CN109846044A (en) * | 2019-01-31 | 2019-06-07 | 山东环亿生物科技有限公司 | With improvement memory, the probiotic composition for improving immunity and preparation method thereof |
| CN109907199A (en) * | 2019-04-03 | 2019-06-21 | 哈工大机器人（山东）智能装备研究院 | A kind of Celery probiotics effervescent tablet and preparation method thereof |
| CN110787190A (en) * | 2019-12-17 | 2020-02-14 | 杨波 | Compound probiotic composition with anti-aging effect and preparation method and application thereof |
| CN111109596A (en) * | 2020-02-07 | 2020-05-08 | 张志红 | Health product for enhancing immunity and preparation method thereof |
| CN111743158A (en) * | 2020-06-08 | 2020-10-09 | 杭州娃哈哈科技有限公司 | Probiotic tablet with function of enhancing immunity and preparation method thereof |
| CN111743158B (en) * | 2020-06-08 | 2022-12-23 | 杭州娃哈哈科技有限公司 | Probiotic tablet with function of enhancing immunity and preparation method thereof |
| CN117487725A (en) * | 2023-12-29 | 2024-02-02 | 微康益生菌(苏州)股份有限公司 | Lactobacillus acidophilus with immunity regulating effect and application thereof |
| CN117487725B (en) * | 2023-12-29 | 2024-03-12 | 微康益生菌(苏州)股份有限公司 | Lactobacillus acidophilus with immunity regulating effect and application thereof |
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Application publication date: 20160608 |