CN102329759A - Method for improving freeze-drying survival rate of Lactobacillus acidophilus - Google Patents
Method for improving freeze-drying survival rate of Lactobacillus acidophilus Download PDFInfo
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- CN102329759A CN102329759A CN201110310289A CN201110310289A CN102329759A CN 102329759 A CN102329759 A CN 102329759A CN 201110310289 A CN201110310289 A CN 201110310289A CN 201110310289 A CN201110310289 A CN 201110310289A CN 102329759 A CN102329759 A CN 102329759A
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Abstract
The invention aims to provide a method for improving the freeze-drying survival rate of Lactobacillus acidophilus. The method comprises the following steps of: culturing the Lactobacillus acidophilus for 18h at the temperature of 37 DEG C, performing sublethal treatment on the Lactobacillus acidophilus, centrifuging for 10 to 15min at the temperature of 4 DEG C at the rotating speed of 7,000rpm, adding skimmed milk and a phosphate buffer solution into bacterial sludge of the Lactobacillus acidophilus, pre-freezing for 8 to 12h at the temperature of -40 DEG C, and putting into a freeze dryer and freeze-drying for 18 to 24h at the temperature of between -50 and -60 DEG C under the vacuum degree of between 4 and 6Pa to obtain bacterial powder of the Lactobacillus acidophilus. In the method for improving the freeze-drying survival rate of the Lactobacillus acidophilus, a Lactobacillus acidophilus culture solution is subjected to cold shock, heat shock or acid shock treatment first so as to ensure that the Lactobacillus acidophilus induces to synthesize cold shock protein and heat shock protein under adverse circumstances or changes the composition of fatty acid of cell membranes, so that the resistance of the Lactobacillus acidophilus in the subsequent freeze-drying process is improved, and the freeze-drying survival rate and the viable count of the bacterial powder are improved.
Description
Technical field
The invention belongs to Lactobacterium acidophilum production of articles technical field, relate to a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate.
Background technology
Lactobacterium acidophilum (Lactobacillus acidophilus) belongs to the lactobacillus in the lactobacillaceae, and form is elongated rod-shaped, and the cell size is generally 0.6～0.9*1.5～6.0 μ m, and Cheng Dan, two perhaps 2-3 the one-tenth short chains of one-tenth are arranged.Atrichia does not move, Gram-positive, and amphimicrobian is present in the gi tract of humans and animals natively, in people's oral cavity, vagina and the traditional fermented-milk.Optimum growth temperature is 35 ℃～38 ℃, do not grow below 20 ℃, and 43 ℃～48 ℃ of maximum growth temperatures, poor heat resistance can be utilized glucose, fructose, lactose, sucrose to carry out homofermentation and produce DL type lactic acid.
Lactobacterium acidophilum is one of probiotic bacterium commonly used in milk-product and the preparation thereof, and it has the lactose intolerance of alleviation and irritable bowel syndrome, prevention diarrhoea or constipation; Reconcile the human intestinal microflora balance; Reduce blood cholesterol, enhancing body immunizing power suppresses functions such as tumour generation; Be widely used at present the production of functional dairy product, protective foods and probiotics, it is necessary as the raw material of starter or protective foods therefore to prepare bifidus bacillus bacterium powder.
Vacuum freeze-drying method is mainly adopted in the preparation of Lactobacterium acidophilum bacterium powder at present, but in freezing dry process, Lactobacterium acidophilum will be stood freezing and dry two kinds of fierce effect of factors, causes thalline dead.Domestic research to freeze-dried Lactobacillus acidophilus powder at present mainly concentrates on the screening aspect of lyophilized vaccine in the preparation process, and has obtained various frozen-dried protective agent prescriptions, but does not handle the method that improves the lactobacillus acidophilus freeze-drying survival rate through inferior causing death.
Summary of the invention
The object of the present invention is to provide a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate, this method can solve the freeze-drying survival rate that Lactobacterium acidophilum exists in freeze-drying process low, the problem that viable count is few.
For achieving the above object, the present invention has adopted following technical scheme:
Lactobacterium acidophilum is cultivated 18h in 37 ℃ in MRS meat soup; Stop cultivating the back Lactobacterium acidophilum is carried out the deadly processing in Asia; Viable count is surveyed in sampling, and at 4 ℃, the centrifugal 10-15min of 7000rpm, abandoning supernatant gets Lactobacterium acidophilum bacterium mud then; In Lactobacterium acidophilum bacterium mud, add behind the phosphoric acid buffer of 10-20% skimming milk and bacterium shale amount 80-90%, pH6.8 of bacterium shale amount at-40 ℃ of following pre-freeze 8-12h; Place freeze drier in temperature-50-60 ℃ then, vacuum tightness is freeze-drying 18-24h under the 4-6Pa, promptly gets Lactobacterium acidophilum bacterium powder.
The total mass of said skimming milk and phosphoric acid buffer equates with the quality of Lactobacterium acidophilum bacterium mud.
Said Asia causes death to handle and is meant cold shock.
Said cold shock is meant Lactobacterium acidophilum is left standstill 0.5-1.5h at 8-10 ℃.
Said Asia causes death to handle and is meant heat-shocked.
Said heat-shocked is meant Lactobacterium acidophilum is left standstill 1h at 38-42 ℃.
Said Asia causes death to handle and is meant the acid shock.
Said acid shock is meant that the pH that uses sulfuric acid adjusting nutrient solution makes Lactobacterium acidophilum leave standstill 30min in 5-8 ℃ after as 4.0-5.5.
The method of raising lactobacillus acidophilus freeze-drying survival rate according to the invention is handled Lactobacterium acidophilum earlier through inferior causing death; Make Lactobacterium acidophilum in adverse circumstance, induce synthetic cold shock protein, heat shock protein(HSP) or change cell membrane fat acid and form; Thereby improve the resistibility of Lactobacterium acidophilum in follow-up freeze-drying process, improve freeze-drying survival rate and bacterium powder viable count.
Embodiment
Below in conjunction with embodiment the present invention is further specified.
Design of the present invention is such: the Lactobacterium acidophilum nutrient solution is handled through cold shock, heat-shocked or acid shock earlier; Make Lactobacterium acidophilum in adverse circumstance, induce synthetic cold shock protein, heat shock protein(HSP) or change cell membrane fat acid and form; Thereby improve the resistibility of Lactobacterium acidophilum in follow-up freeze-drying process, improve freeze-drying survival rate and bacterium powder viable count.Concrete grammar is following: (1) at first with Lactobacterium acidophilum 37 ℃ of cultivation 18h in MRS meat soup, stops then cultivating, and nutrient solution is left standstill 0.5-1.5h at 8-10 ℃; Viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 10min-15min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, after bacterium mud adds skimming milk and phosphoric acid buffer at-40 ℃ of following pre-freeze 8-12h; Place freeze drier to carry out freeze-drying then; At temperature-50--60 ℃, vacuum tightness is freeze-drying 18-24h under the 4-6Pa, can obtain Lactobacterium acidophilum bacterium powder.(2) at first with Lactobacterium acidophilum 37 ℃ of cultivation 18h in MRS meat soup, stop then cultivating, and nutrient solution is left standstill 1h at 38-42 ℃, viable count is surveyed in sampling; Then at 4 ℃, the centrifugal 10min of 7000rpm, abandoning supernatant; Obtain Lactobacterium acidophilum bacterium mud, after bacterium mud adds skimming milk and phosphoric acid buffer,, place freeze drier to carry out freeze-drying then at-40 ℃ of following pre-freeze 8-12h; At temperature-50-60 ℃, vacuum tightness is freeze-drying 18-24h under the 4-6Pa, can obtain Lactobacterium acidophilum bacterium powder.(3) at first with Lactobacterium acidophilum in MRS meat soup 37 ℃ cultivate 18h, with sulfuric acid the pH of nutrient solution is transferred to 4.0-5.5 then, 5-8 ℃ leaves standstill 30min; Viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 10min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, adds skimming milk and phosphoric acid buffer at-40 ℃ of following pre-freeze 8-12h at bacterium mud; Place freeze drier to carry out freeze-drying then; At temperature-50-60 ℃, vacuum tightness is freeze-drying 18-24h under the 4-6Pa, can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 1-1
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 1.0h at 9 ℃, viable count is surveyed in sampling, then at 4 ℃; The centrifugal 10min of 7000rpm, abandoning supernatant obtains Lactobacterium acidophilum bacterium mud; The bacterium mud of weighing adds the skimmed milk powder of bacterium mud weight 20% (W/W), adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 80% (W/W) again; At-40 ℃ of following pre-freeze 8h, place freeze drier to carry out freeze-drying then then, 4-6Pa ,-50 ℃ of following freeze-drying 24h; Can obtain Lactobacterium acidophilum bacterium powder, its freeze-drying survival rate is 70.45% (control group is 46.11%), and viable count is 2.1 * 10
11((control group is 1.10 * 10 to cfu/g
11Cfu/g)).
Embodiment 1-2
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 0.5h at 8 ℃, viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 15min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 10% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 90% (W/W), more then at-40 ℃ of following pre-freeze 12h; Place freeze drier to carry out freeze-drying then, 6Pa ,-60 ℃ of following freeze-drying 21h can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 1-3
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 1.5h at 10 ℃, viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 11min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 16% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 84% (W/W), more then at-40 ℃ of following pre-freeze 11h; Place freeze drier to carry out freeze-drying then, 4Pa ,-52 ℃ of following freeze-drying 18h can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 2-1
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 1.0h at 39 ℃, viable count is surveyed in sampling, then at 4 ℃; The centrifugal 10min of 7000rpm, abandoning supernatant obtains Lactobacterium acidophilum bacterium mud; The bacterium mud of weighing adds the skimmed milk powder of bacterium mud weight 15% (W/W), adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 85% (W/W) again; At-40 ℃ of following pre-freeze 12h, place freeze drier to carry out freeze-drying then then, 4-6Pa ,-50 ℃ of following freeze-drying 20h; Can obtain Lactobacterium acidophilum bacterium powder, its freeze-drying survival rate is 84.25% (control group is 49.64%), and viable count is 2.61 * 10
11(control group is 1.13 * 10 to cfu/g
11Cfu/g).
Embodiment 2-2
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 1.0h at 38 ℃, viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 13min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 20% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 80% (W/W), more then at-40 ℃ of following pre-freeze 9h; Place freeze drier to carry out freeze-drying then, 6Pa ,-52 ℃ of following freeze-drying 18h can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 2-3
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; Then nutrient solution is left standstill 1.0h at 42 ℃, viable count is surveyed in sampling, then at 4 ℃, and the centrifugal 15min of 7000rpm; Abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 10% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 90% (W/W), more then at-40 ℃ of following pre-freeze 8h; Place freeze drier to carry out freeze-drying then, 4Pa ,-60 ℃ of following freeze-drying 24h can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 3-1
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; With the sulfuric acid of 2mol/L the pH of nutrient solution is transferred to 4.5,5 ℃ then and leave standstill 30min, viable count is surveyed in sampling, then at 4 ℃; The centrifugal 10min of 7000rpm, abandoning supernatant obtains Lactobacterium acidophilum bacterium mud; The bacterium mud of weighing adds the skimmed milk powder of bacterium mud weight 10% (W/W), adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 90% (W/W) again; At-40 ℃ of following pre-freeze 10h, place freeze drier to carry out freeze-drying then then, 4-6Pa ,-60 ℃ of following freeze-drying 22h; Can obtain Lactobacterium acidophilum bacterium powder, its freeze-drying survival rate is 87.22% (contrast is 49.64%), and viable count is 4.09 * 10
11(control group is 1.07 * 10 to cfu/g
11Cfu/g).
Embodiment 3-2
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; With the sulfuric acid of 2mol/L the pH of nutrient solution is transferred to 4.0,7 ℃ then and leave standstill 30min, viable count is surveyed in sampling, then at 4 ℃; The centrifugal 12min of 7000rpm, abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 14% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 86% (W/W), more then at-40 ℃ of following pre-freeze 8h; Place freeze drier to carry out freeze-drying then, 4Pa ,-50 ℃ of following freeze-drying 24h can obtain Lactobacterium acidophilum bacterium powder.
Embodiment 3-3
MRS meat soup at 118 ℃ of 15min that sterilize down, is cooled to room temperature, inserts Lactobacterium acidophilum, 37 ℃ of constant temperature culture 18h by 4% (V/V) inoculum size then; With the sulfuric acid of 2mol/L the pH of nutrient solution is transferred to 5.5,8 ℃ then and leave standstill 30min, viable count is surveyed in sampling, then at 4 ℃; The centrifugal 15min of 7000rpm, abandoning supernatant obtains Lactobacterium acidophilum bacterium mud, the bacterium mud of weighing; The skimmed milk powder that adds bacterium mud weight 20% (W/W) adds the phosphoric acid buffer of the pH6.8 of bacterium mud weight 80% (W/W), more then at-40 ℃ of following pre-freeze 12h; Place freeze drier to carry out freeze-drying then, 6Pa ,-53 ℃ of following freeze-drying 18h can obtain Lactobacterium acidophilum bacterium powder.
In the foregoing description, control group refers to that other conditionally complete is identical, does not just handle through inferior causing death.
Claims (8)
1. method that improves the lactobacillus acidophilus freeze-drying survival rate is characterized in that: may further comprise the steps:
Lactobacterium acidophilum is cultivated 18h in 37 ℃ in MRS meat soup; Stop cultivating the back Lactobacterium acidophilum is carried out the deadly processing in Asia; At 4 ℃, the centrifugal 10-15min of 7000rpm, abandoning supernatant gets Lactobacterium acidophilum bacterium mud then, in Lactobacterium acidophilum bacterium mud, adds behind the phosphoric acid buffer of 10-20% skimming milk and bacterium shale amount 80-90%, pH6.8 of bacterium shale amount at-40 ℃ of following pre-freeze 8-12h; Place freeze drier in temperature-50-60 ℃ then, vacuum tightness is freeze-drying 18-24h under the 4-6Pa.
2. according to the said a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate of claim 1, it is characterized in that: the total mass of said skimming milk and phosphoric acid buffer equates with the quality of Lactobacterium acidophilum bacterium mud.
3. according to claim 1 or 2 said a kind of methods that improve the lactobacillus acidophilus freeze-drying survival rate, it is characterized in that: said Asia causes death to handle and is meant cold shock.
4. according to the said a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate of claim 3, it is characterized in that: said cold shock is meant Lactobacterium acidophilum is left standstill 0.5-1.5h at 8-10 ℃.
5. according to claim 1 or 2 said a kind of methods that improve the lactobacillus acidophilus freeze-drying survival rate, it is characterized in that: said Asia causes death to handle and is meant heat-shocked.
6. according to the said a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate of claim 5, it is characterized in that: said heat-shocked is meant Lactobacterium acidophilum is left standstill 1h at 38-42 ℃.
7. according to claim 1 or 2 said a kind of methods that improve the lactobacillus acidophilus freeze-drying survival rate, it is characterized in that: said Asia causes death to handle and is meant the acid shock.
8. according to the said a kind of method that improves the lactobacillus acidophilus freeze-drying survival rate of claim 7, it is characterized in that: said acid shock is meant that the pH that uses sulfuric acid adjusting nutrient solution makes Lactobacterium acidophilum leave standstill 30min in 5-8 ℃ after as 4.0-5.5.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757923A (en) * | 2012-07-30 | 2012-10-31 | 陕西省科学院酶工程研究所 | Method for preparing bifidobacterium bifidum powder with high viable count |
| CN108935709A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk piece and preparation method thereof |
| CN108935703A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of compound probiotic goat milk powder and preparation method thereof |
| CN108935704A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk powder and preparation method thereof |
| CN111575223A (en) * | 2020-05-20 | 2020-08-25 | 江南大学 | Method for reducing secretion of surface substances of lactobacillus rhamnosus |
| CN114686407A (en) * | 2022-05-13 | 2022-07-01 | 微康益生菌(苏州)股份有限公司 | Preparation method of lactobacillus acidophilus powder for improving culturable cell content |
| CN116396904A (en) * | 2023-04-04 | 2023-07-07 | 陕西科技大学 | Preparation of probiotics powder and application of probiotics powder in goat milk powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875911A (en) * | 2010-06-23 | 2010-11-03 | 陕西科技大学 | Protective agent in the freeze drying process of Lactobacillus acidophilus |
| CN101962616A (en) * | 2010-09-29 | 2011-02-02 | 陕西科技大学 | Method for preparing lactobacillus acidophilus powder by freeze-drying in vacuum |
-
2011
- 2011-10-13 CN CN201110310289.3A patent/CN102329759B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875911A (en) * | 2010-06-23 | 2010-11-03 | 陕西科技大学 | Protective agent in the freeze drying process of Lactobacillus acidophilus |
| CN101962616A (en) * | 2010-09-29 | 2011-02-02 | 陕西科技大学 | Method for preparing lactobacillus acidophilus powder by freeze-drying in vacuum |
Non-Patent Citations (9)
| Title |
|---|
| A.KILARA等: "Effect of cryoprotective agents on freeze-drying and strorage of lactic culture", 《CULT. DAIRY PROD.》 * |
| HSIAO WAN-LING等: "Heat shock effects on the viability of Cronobacter sakazakii during the dehydration, fermentation, and storage of lactic cultured milk products", 《FOOD MICROBIOLOGY》 * |
| M. SAARELA等: "Influence of fermentation time, cryoprotectant and neutralization of cell concentrate on freeze-drying survival, storage stability, and acid and bile exposure of Bifidobacterium animalis ssp. lactis cells produced without milk-based ingredients", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
| ZDENEK HUBALEK: "Protectants used in the cryopreservation of microorganisms", 《SCIENCE DIRECT》 * |
| 徐丽萍: "嗜酸乳杆菌冻干菌粉保护剂选择的研究", 《食品工业科技》 * |
| 朱东升等: "嗜酸乳杆菌冷冻干燥过程中保护剂的筛选及液氮预冻", 《食品科学》 * |
| 李春等: "两种预处理方法对冷藏期乳酸菌存活率的影响", 《食品科学》 * |
| 李春等: "乳酸菌细胞膜冻干损伤研究进展", 《中国乳品工业》 * |
| 赵瑞香等: "嗜酸乳杆菌冷冻干燥过程中保护剂的选择", 《食品科学》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757923A (en) * | 2012-07-30 | 2012-10-31 | 陕西省科学院酶工程研究所 | Method for preparing bifidobacterium bifidum powder with high viable count |
| CN102757923B (en) * | 2012-07-30 | 2014-04-02 | 陕西省科学院酶工程研究所 | Method for preparing bifidobacterium bifidum powder with high viable count |
| CN108935709A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk piece and preparation method thereof |
| CN108935703A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of compound probiotic goat milk powder and preparation method thereof |
| CN108935704A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk powder and preparation method thereof |
| CN111575223A (en) * | 2020-05-20 | 2020-08-25 | 江南大学 | Method for reducing secretion of surface substances of lactobacillus rhamnosus |
| CN111575223B (en) * | 2020-05-20 | 2022-04-15 | 江南大学 | Method for reducing secretion of surface substances of lactobacillus rhamnosus |
| CN114686407A (en) * | 2022-05-13 | 2022-07-01 | 微康益生菌(苏州)股份有限公司 | Preparation method of lactobacillus acidophilus powder for improving culturable cell content |
| CN114686407B (en) * | 2022-05-13 | 2022-09-06 | 微康益生菌(苏州)股份有限公司 | Preparation method of lactobacillus acidophilus powder for improving culturable cell content |
| CN116396904A (en) * | 2023-04-04 | 2023-07-07 | 陕西科技大学 | Preparation of probiotics powder and application of probiotics powder in goat milk powder |
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