CN109527096A - A kind of probiotic formulation goat milk powder and preparation method thereof - Google Patents

A kind of probiotic formulation goat milk powder and preparation method thereof Download PDF

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Publication number
CN109527096A
CN109527096A CN201811620591.7A CN201811620591A CN109527096A CN 109527096 A CN109527096 A CN 109527096A CN 201811620591 A CN201811620591 A CN 201811620591A CN 109527096 A CN109527096 A CN 109527096A
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goat milk
powder
parts
milk powder
preparation
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舒国伟
殷春
李宏
陈合
万红昌
王旭
张萌
郭余粮
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Shaanxi Ya Tai Dairy Industry Co Ltd
Shaanxi University of Science and Technology
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Shaanxi Ya Tai Dairy Industry Co Ltd
Shaanxi University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/158—Milk preparations; Milk powder or milk powder preparations containing additives containing vitamins or antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of probiotic formulation goat milk powder and preparation method thereof, probiotic formulation goat milk powder is made of fresh sheep cream or full-cream goat milk powder, desalted whey powder, whey protein concentrate, stachyose, vitamin C, sheep cream throw type leaven and probiotics bacterial powder；Preparation method are as follows: the pretreated fresh goat milk of mixing, desalted whey powder, whey protein concentrate, stachyose and vitamin C, sterilizing, which is concentrated and is spray-dried, obtains prebiotics goat milk powder, then adds newborn throw type leaven and probiotics bacterial powder and mix.The present invention promotes the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increases intestines peristalsis, the functions such as prevent constipation, to be beneficial to consumer health, promote the growth of probiotics when restoring and preparing fermentation sheep cream, the viable count of probiotics is improved, the stability of sheep cream throw type leaven and probiotics bacterial powder in milk powder also can be improved in the vitamin C of addition.

Description

A kind of probiotic formulation goat milk powder and preparation method thereof
Technical field
The invention belongs to functional goat milk powder and preparation method thereof technical field, be related to a kind of probiotic formulation goat milk powder and Preparation method.
Background technique
Goat milk have protein composition rationally, protein be not easy allergy, goat milk fat it is easy to digest absorb, calcium content is high, The advantages that rich in bioactive substance, epithelial cell growth factor such as identical with people's milk, to maintenance alimentary canal health and skin Skin plays a significant role, and goat milk also contains there are many nucleotide, and cyclic adenosine monophosphate (cAMP) content is 2 times of milk, can promote lactose Digestibility and utilization；Also contain insulin-like growth factor in goat milk, has certain effect to diabetes tool, here it is Compendium of Material Medica Goat milk on the books " can be controlled and be quenched one's thirst " in " haigoushen ", therefore goat milk all has very infant, pregnant woman, old man and patient Good nutrition and health care effect.
Probiotics refers to the microorganism beneficial to human body living, advantageous to the health of host when giving sufficient dosage.I State develops probiotic bacteria milk product, as " hat benefit cream " of Mongolia Ox, bright " AB100 ", " beneficial bacteria is more " of ternary, Erie it is " light Fruit is inspired ", " the increasingly bacterium yoghurt " of Long Dan etc., compared with foreign countries, probiotic bacteria milk product is mainly acidified milk or milk beverage etc. Liquid diary product, probiotics milk powder product are few.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, present invention solves the technical problem that being to provide a kind of probiotic formulation Goat milk powder and preparation method thereof, realizes the powdered of probiotics goat milk product, and probiotic bacteria milk product is no longer limited to as fermentation The liquid diary products such as cream or milk beverage.
The present invention is to be achieved through the following technical solutions:
A kind of probiotic formulation goat milk powder, by mass fraction by 5600-6400 parts of fresh sheep creams or 700-800 parts of full-cream goat milk Powder, 108-249 part desalted whey powder, 30-50 parts of whey protein concentrate, 20-40 parts of stachyoses, 0.3-1 parts of vitamin Cs, 0.5-1 Part sheep cream throw type leaven and 0.005-0.01 parts of probiotics bacterial powder compositions.
A kind of preparation method of probiotic formulation goat milk powder, comprising the following steps:
Step 1, prepared by sheep cream throw type leaven: lactobacillus bulgaricus powder and streptococcus thermophilus bacterium powder being mixed, made The ratio between its viable count is that 1:1 obtains sheep cream throw type leaven；
Step 2, prepared by prebiotics goat milk powder: adding 108-249 parts into 5600-6400 parts of fresh goat milk by mass fraction and takes off Salt whey powder, 30-50 part whey protein concentrate, 20-40 parts of stachyoses and 0.3-1 parts of vitamin Cs, stir evenly；It is concentrated into solid Shape object content is the concentrated milk of 46-53%, and concentrated milk spray drying obtains prebiotics goat milk powder or in mass ratio to 700-800 108-249 parts of desalted whey powders, 30-50 parts of whey protein concentrate, 20-40 parts of stachyoses and 0.3-1 are added in the full-cream goat milk of part Part vitamin C is uniformly mixed, and obtains prebiotics goat milk powder；
Step 3, prepared by probiotic formulation goat milk powder: it is directly putting type fermented that 0.5-1 parts of sheep creams are added into prebiotics goat milk powder Agent and 0.005-0.01 parts of probiotics bacterial powder are to get probiotic formulation goat milk powder.
Further, in step 1 lactobacillus bulgaricus powder preparation: by mass volume ratio to mass concentration be 10% 0.3% casein hydrolysate, 0.15% sodium acetate and 0.25% Triammonium citrate are added in Longstamen Onion Bulb extracting solution, are sterilized so cooling that train Support base；3% lactobacillus bulgaricus that has activated of access into culture medium by volume, in 37 DEG C of constant temperature incubation 20h, freeze from The heart obtains lactobacillus bulgaricus bacterium mud, then adds the compound freeze drying protectant of 1.2 times of lactobacillus bulgaricus bacterium mud weight, 10 DEG C of cold treatment 1.5h are subsequently placed in, then use vacuum freeze drier freeze-drying for 24 hours to get viable count for 3.6 × 1011CFU/ G lactobacillus bulgaricus bacterium powder.
Further, in step 1 streptococcus thermophilus bacterium powder preparation: by mass volume ratio to mass concentration be 10% puncture Add 1.5% glucose in Chenopodiaceae extracting solution, 0.25% soy peptone, 1% casein hydrolysate, 0.0015% glutamic acid, 0.2%KH2PO4, 0.05% Tween-80 obtains culture medium, pH is adjusted to 6.8；Accessed by volume into culture medium activated it is thermophilic Hot streptococcus, in 42 DEG C of constant temperature incubation 9h, refrigerated centrifuge obtains streptococcus thermophilus bacterium mud, the compound jelly of 2 times of bacterium mud weight of addition Dry protective agent is subsequently placed in 12 DEG C of cold treatment 1.5h, is then lyophilized using vacuum freeze drier for 24 hours to get streptococcus thermophilus Bacterium powder, viable count are 2.4 × 1011CFU/g。
Further, in step 2 probiotics bacterial powder preparation: by mass volume ratio to mass concentration be 10% Longstamen Onion Bulb mention It takes and adds 1.2% lactose, 0.5% peptone, 0.25% xylo-oligosaccharide, 0.20% sodium acetate, 0.22% Triammonium citrate in liquid Culture medium is made, medium sterilization is cooling and is respectively connected to the probiotics activated, for 24 hours in 37 DEG C of constant temperature incubations, refrigerated centrifuge Probiotics bacterium mud is obtained, the compound freeze drying protectant of 1.5 times of probiotics bacterium mud weight is added, is placed in 10 DEG C of cold treatment 1.5h, adopts It is lyophilized with vacuum freeze drier for 24 hours to get probiotics bacterial powder.
Further, the preparation of Longstamen Onion Bulb extracting solution: taking the Longstamen Onion Bulb of certain mass, by quality and volume proportion (grams per milliliter) The ratio of 1:10 soaks in water 20-30min, is boiled and boiling 30min is maintained to obtain filtrate with 400 mesh filter-cloth filterings； Filter residue is added distilled water heating in quality and the ratio of volume proportion (grams per milliliter) 1:10 again and boils and maintain 20min, filters； Merge filtrate twice and constant volume, obtains the Longstamen Onion Bulb extracting solution that mass concentration is 10%.
Further, the preparation of probiotics bacterial powder, when cultivating two qi Bifidobacteriums, also addition is pressed after medium sterilization is cooling Mass volume ratio 0.05% passes through the cysteine hydrochloride of 0.22 μm of membrane filtration degerming.
Further, it the preparation of puncture vine extracting solution: takes a certain amount of puncture vine to crush, makes its granularity 20-60 mesh；By quality Deionized water is added with the ratio of volume proportion (grams per milliliter) 1:10, boil and keeps boiling 30min, with the filter cloth mistake of 400 mesh Filter；Filter residue is boiled again in quality and the ratio of volume proportion (grams per milliliter) 1:10 addition deionized water and keeps boiling 30min, filtering；Merge filtrate twice and constant volume, obtains puncture vine extracting solution.
Further, the viable count of probiotics is 1.25 × 10 in probiotic formulation goat milk powder made from step 26CFU/g- 3.5×107CFU/g。
Further, the spray drying condition of step 1 are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, air pressure Power is 1 atmospheric pressure, and hot-air flow velocity is 0.25m3/min。
Compared with prior art, the invention has the following beneficial technical effects:
A kind of probiotic formulation goat milk powder provided by the invention and preparation method thereof, realizes the powder of probiotics goat milk product Endization, probiotic bacteria milk product are no longer limited to as liquid diary products such as acidified milk or milk beverages；The addition of probiotic formulation goat milk powder Stachyose can promote the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increase intestines peristalsis, the functions such as prevent constipation, To be beneficial to consumer health, while stachyose promotes the growth of probiotics when restoring and preparing fermentation sheep cream, improves prebiotic The stability of sheep cream throw type leaven and probiotics bacterial powder in milk powder also can be improved in the viable count of bacterium, the vitamin C of addition.
Specific embodiment
Specific embodiment is given below.
Probiotics bacterial powder in the present invention includes wherein the one of lactobacillus acidophilus, two qi Bifidobacteriums and lactobacillus plantarum Kind, two or three.
Embodiment 1
A kind of probiotic formulation goat milk powder, by mass fraction by 6400 parts of fresh sheep creams, 108 parts of desalted whey powders, 50 parts it is dense Contracting lactalbumin, 40 parts of stachyoses, 1 part of vitamin C, 1 part of sheep cream throw type leaven, 0.01 part of probiotics bacterial powder composition；Its In, in mass ratio, lactobacillus acidophilus powder and two qi Bifidobacterium powder mass ratios are 1:1 in probiotics bacterial powder；
Lactobacillus acidophilus and the number of active bifid bacteria are respectively 1.25 × 10 in probiotic formulation goat milk powder obtained7CFU/ GCFU/g and 1.4 × 107CFU/g。
Sheep cream throw type leaven is made of lactobacillus bulgaricus and streptococcus thermophilus, and the ratio between viable count is 1:1, Total viable count is 2.88 × 1011CFU/g, lactobacillus bulgaricus and streptococcus thermophilus viable count are respectively 1.44 × 1011CFU/g。
The preparation step of probiotic formulation goat milk powder are as follows:
First: the preparation of sheep cream throw type leaven
1. prepared by lactobacillus bulgaricus bacterium powder: adding in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Add casein hydrolysate, sodium acetate, Triammonium citrate, making its additive amount is respectively 0.3%, 0.15%, 0.25%, sterilizing cooling The lactobacillus bulgaricus culture solution activated is accessed by the inoculum concentration of 3% (v/v) afterwards, is terminated in 37 DEG C of constant temperature incubation 20h Culture, refrigerated centrifuge obtain lactobacillus bulgaricus bacterium mud, then add the compound of 1.2 times of lactobacillus bulgaricus bacterium mud weight Freeze drying protectant is subsequently placed in 10 DEG C of cold treatment 1.5h, is then lyophilized using vacuum freeze drier for 24 hours to get Bulgaria Lactobacillus bacterium powder, viable count are 3.6 × 1011CFU/g。
2. prepared by streptococcus thermophilus bacterium powder: 1.5% glucose is added into puncture vine extracting solution by mass volume ratio, 0.25% Soy peptone, 1% casein hydrolysate, 0.0015% glutamic acid, 0.2%KH2PO4, 0.05% Tween-80, and pH is adjusted to 6.8, the thermophilus bacteria culture fluid activated is then accessed by the inoculum concentration of 6% (v/v), in 42 DEG C of constant temperature incubation 9h, freezing Centrifugation obtains streptococcus thermophilus bacterium mud, then adds 2 times of bacterium mud weight of compound freeze drying protectant, is subsequently placed in 12 DEG C of cold treatments 1.5h, then using vacuum freeze drier freeze-drying for 24 hours to get streptococcus thermophilus bacterium powder, viable count is 2.4 × 1011CFU/ g。
3. prepared by sheep cream throw type leaven: lactobacillus bulgaricus and streptococcus thermophilus bacterium powder being mixed by 1:1.5, i.e., Sheep cream throw type leaven is obtained, total viable count is 2.88 × 1011CFU/g, lactobacillus bulgaricus and streptococcus thermophilus viable bacteria Number is respectively 1.44 × 1011CFU/g。
Second, the preparation of probiotics bacterial powder: added in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio 1.2% lactose, 0.5% peptone, 0.25% xylo-oligosaccharide, 0.20% sodium acetate and 0.22% Triammonium citrate (two qis of culture The cysteine hydrochloride that 0.05% is also added when Bifidobacterium, using 0.22 μm of membrane filtration degerming, culture medium subject to sterilization It is added after cooling), the lactobacillus acidophilus activated, two qi bifids are respectively connected to by the inoculum concentration of 2% (v/v) afterwards after sterilization and cooling Bacillus culture solution terminates culture in 37 DEG C of constant temperature incubations for 24 hours, and refrigerated centrifuge obtains lactobacillus acidophilus, two qi Bifidobacterium bacterium muds, Then the compound freeze drying protectant for adding 1.5 times of bacterium mud weight, is subsequently placed in 10 DEG C of cold treatment 1.5h, then uses vacuum refrigeration For 24 hours to get lactobacillus acidophilus and two qi Bifidobacteriums, lactobacillus acidophilus bacterium powder viable count is 2.5 × 10 for drying machine freeze-drying11CFU/ G, two qi Bifidobacterium powder viable counts are 2.8 × 1011CFU/g。
Third, the preparation of prebiotics goat milk powder: 6400 parts of fresh fresh goat milk of nonreactive in mass ratio after pretreatment, add 108 Part desalted whey powder, 50 parts of whey protein concentrate, 40 parts of stachyoses and 1 part of vitamin C, stir evenly, then by it through superelevation The concentrated milk that solid content is 50% is concentrated under reduced pressure into after cooling down after warm instantaneous sterilizing, concentrated milk is after homogeneous by doing by spraying Dry (spray drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, stream of hot air Speed is 0.25m3/ min) obtain prebiotics goat milk powder.
4th, the preparation of probiotic formulation goat milk powder: added in prebiotics goat milk powder 1 part of sheep cream throw type leaven and 0.01 part of probiotics bacterial powder (in mass ratio, being made of lactobacillus acidophilus powder and bifidobacterium bifidum powder, its ratio be 1:1), i.e., Probiotics formula goat milk powder, in probiotic formulation goat milk powder lactobacillus acidophilus and the number of active bifid bacteria be respectively 1.25 × 107CFU/g and 1.4 × 107CFU/g。
Embodiment 2
By mass fraction by 5600 parts of fresh sheep creams, 249 parts of desalted whey powders, 30 parts of whey protein concentrate, 20 parts of stachyoses, 0.3 part of vitamin C, 0.5 part of sheep cream throw type leaven and 0.005 part of probiotics bacterial powder composition.
The preparation step of probiotic formulation goat milk powder are as follows:
1. sheep cream throw type leaven is the same as embodiment 1；The culture medium of probiotics bacterial powder preparation is with embodiment 1, in culture medium Lactobacillus acidophilus, bifidobacterium bifidum and lactobacillus plantarum are accessed, lactobacillus acidophilus powder, bifidobacterium bifidum powder and plant is made Lactobacillus powder.
2. prepared by prebiotics goat milk powder: adding 249 parts of desalted wheys after pretreatment for 5600 parts of the fresh goat milk of fresh nonreactive Powder, 30 parts of whey protein concentrate, 20 parts of stachyoses and 0.3 part of vitamin C, stir evenly, and then it instantaneously goes out through superhigh temperature The concentrated milk that solid content is 48% is concentrated under reduced pressure into after cooling down after bacterium, concentrated milk is (spraying by spray drying after homogeneous Drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain prebiotics goat milk powder.
Then 0.5 part of sheep cream throw type leaven is added in prebiotics goat milk powder and 0.005 part of probiotics bacterial powder (presses matter Ratio is measured, lactobacillus acidophilus powder, bifidobacterium bifidum powder and lactobacillus plantarum powder ratio are 2:2:1), it is uniformly mixed to get prebiotic Bacterium formula goat milk powder, lactobacillus acidophilus, bifidobacterium bifidum and lactobacillus plantarum viable count difference in probiotic formulation goat milk powder It is 1.0 × 107CFU/g、1.12×107CFU/g and 0.7 × 107CFU/g。
Embodiment 3
A kind of probiotic formulation goat milk powder: by mass fraction by 6000 parts of fresh sheep creams, 169 parts of desalted whey powders, 40 parts it is dense Contracting lactalbumin, 40 parts of stachyoses, 0.5 part of vitamin C, 0.5 part of sheep cream throw type leaven and 0.01 part of probiotics bacterial powder group At.
The preparation step of probiotic formulation goat milk powder are as follows:
1. sheep cream throw type leaven is the same as embodiment 1；The culture medium of probiotics bacterial powder preparation is with embodiment 1, in culture medium Bifidobacterium bifidum and lactobacillus plantarum are accessed, bifidobacterium bifidum powder and lactobacillus plantarum powder is made.
2. prepared by prebiotics goat milk powder: adding 169 parts of desalted wheys after pretreatment for 6000 parts of the fresh goat milk of fresh nonreactive Powder, 40 parts of whey protein concentrate, 40 parts of stachyoses and 0.5 part of vitamin C, stir evenly, and then it instantaneously goes out through superhigh temperature The concentrated milk that solid content is 48% is concentrated under reduced pressure into after cooling down after bacterium, concentrated milk is (spraying by spray drying after homogeneous Drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain prebiotics goat milk powder；
Then 0.5 part of sheep cream throw type leaven is added in prebiotics goat milk powder and 0.01 part of probiotics bacterial powder (presses matter Ratio is measured, bifidobacterium bifidum powder and lactobacillus plantarum powder ratio are 1:1) to get probiotic formulation goat milk powder.Probiotic formulation sheep Bifidobacterium bifidum and lactobacillus plantarum viable count are respectively 1.44 × 10 in milk powder7CFU/g and 1.75 × 107CFU/g。
Embodiment 4
By mass fraction by 5600 parts of fresh sheep creams, 249 parts of desalted whey powders, 30 parts of whey protein concentrate, 20 parts of stachyoses, 0.3 part of vitamin C, 0.5 part of sheep cream throw type leaven and 0.005 part of probiotics bacterial powder composition.
The preparation step of probiotic formulation goat milk powder are as follows:
1. sheep cream throw type leaven is the same as embodiment 1；Probiotics bacterial powder is prepared with embodiment 2；
2. prepared by prebiotics goat milk powder: adding 249 parts of desalted wheys after pretreatment for 5600 parts of the fresh goat milk of fresh nonreactive Powder, 30 parts of whey protein concentrate, 20 parts of stachyoses and 0.3 part of vitamin C, stir evenly, and then it instantaneously goes out through superhigh temperature The concentrated milk that solid content is 46% is concentrated under reduced pressure into after cooling down after bacterium, concentrated milk is (spraying by spray drying after homogeneous Drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain prebiotics goat milk powder.
Then 0.5 part of sheep cream throw type leaven is added in prebiotics goat milk powder and 0.005 part of probiotics bacterial powder (presses matter Ratio is measured, lactobacillus acidophilus powder, bifidobacterium bifidum powder and lactobacillus plantarum powder ratio are 2:2:1), it is uniformly mixed to get prebiotic Bacterium formula goat milk powder, lactobacillus acidophilus, bifidobacterium bifidum and lactobacillus plantarum viable count difference in probiotic formulation goat milk powder It is 1.0 × 107CFU/g、1.12×107CFU/g and 0.7 × 107CFU/g。
Embodiment 5
A kind of probiotic formulation goat milk powder: by mass fraction by 6000 parts of fresh sheep creams, 169 parts of desalted whey powders, 40 parts it is dense Contracting lactalbumin, 40 parts of stachyoses, 0.5 part of vitamin C, 0.5 part of sheep cream throw type leaven and 0.01 part of probiotics bacterial powder group At.
The preparation step of probiotic formulation goat milk powder are as follows:
1. sheep cream throw type leaven is the same as embodiment 1；Probiotics bacterial powder is prepared with embodiment 3；
2. prepared by prebiotics goat milk powder: adding 169 parts of desalted wheys after pretreatment for 6000 parts of the fresh goat milk of fresh nonreactive Powder, 40 parts of whey protein concentrate, 40 parts of stachyoses and 0.5 part of vitamin C, stir evenly, and then it instantaneously goes out through superhigh temperature The concentrated milk that solid content is 53% is concentrated under reduced pressure into after cooling down after bacterium, concentrated milk is (spraying by spray drying after homogeneous Drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, and hot-air flow velocity is 0.25m3/ min) obtain prebiotics goat milk powder；
Then 0.5 part of sheep cream throw type leaven is added in prebiotics goat milk powder and 0.01 part of probiotics bacterial powder (presses matter Ratio is measured, bifidobacterium bifidum powder and lactobacillus plantarum powder ratio are 1:1) to get probiotic formulation goat milk powder.Probiotic formulation sheep Bifidobacterium bifidum and lactobacillus plantarum viable count are respectively 1.44 × 10 in milk powder7CFU/g and 1.75 × 107CFU/g。
Embodiment 6
A kind of probiotic formulation goat milk powder, by mass fraction by 720 parts of full-cream goat milk powders, 199 parts of desalted whey powders, 45 parts Whey protein concentrate, 35 parts of stachyoses, 0.5 part of vitamin C, 0.5 part of sheep cream throw type leaven, 0.006 part of probiotics bacterial powder (in mass ratio, the ratio between lactobacillus acidophilus powder, bifidobacterium bifidum powder, lactobacillus plantarum powder are 1:1:1) composition.
The preparation step of probiotic formulation goat milk powder are as follows:
1. sheep cream throw type leaven is the same as embodiment 1；Probiotics bacterial powder is prepared with embodiment 2；
2. the preparation of probiotic formulation goat milk powder: by 0.5 part of vitamin C, 0.5 part of sheep cream throw type leaven and 0.006 Part probiotics bacterial powder (in mass ratio, the ratio between lactobacillus acidophilus powder, bifidobacterium bifidum powder, lactobacillus plantarum powder are 1:1:1) exists It is mixed uniformly rear by mass fraction addition 35 parts of stachyoses, 45 parts of whey protein concentrate, 199 parts of desalted whey powders under aseptic condition With 720 parts of full-cream goat milk powders, after mixing up to probiotic formulation goat milk powder.Acidophilus cream bar in probiotic formulation goat milk powder Bacterium, bifidobacterium bifidum, lactobacillus plantarum viable count are respectively 8.33 × 106CFU/g、9.33×106CFU/g and 1.27 × 107CFU/g。
Probiotic formulation goat milk powder eating method obtained in embodiment 1-6:
Drunk 1. directly reconstituting: in 1:(6-8 in probiotic formulation goat milk powder) ratio temperature is added is 40-45 DEG C Warm water stirs evenly i.e. drinkable.
2. being eaten after fermentation: in 1:(6-7 in probiotic formulation goat milk powder) ratio temperature is added as 40-45 DEG C of temperature Boiled water stirring, is subsequently placed in Yogurt making machine the 4-10h that ferments to its curdled milk, then up to fermentation sheep after placing it in refrigerator cold-storage overnight Cream, direct-edible or addition fruit etc. are edible.
Wherein, optimization, the preparation of Longstamen Onion Bulb extracting solution Longstamen Onion Bulb extracting solution: taking the Longstamen Onion Bulb of certain mass, then by quality with The ratio of volume proportion (grams per milliliter) 1:10 soaks in water 20-30min, is then added into and boils, and maintains boiling 30min, Again with 400 mesh filter-cloth filterings, filtrate is obtained, then again by again by quality with volume proportion (grams per milliliter) 1:10's in filter residue Ratio is added distilled water heating and boils and maintain 20min, is then filtered, merges filtrate twice, make Longstamen Onion Bulb and water ratio 1:10 (i.e. 10ml extracting solution is made in 1g Longstamen Onion Bulb altogether), i.e. mass concentration be 10% Longstamen Onion Bulb extracting solution.
The preparation of puncture vine extracting solution: taking a certain amount of puncture vine to crush, and makes its granularity 20-60 mesh, then press quality and volume Deionized water is added in the ratio for matching (grams per milliliter) 1:10, boils with high heat on electric furnace, and then mild fire keeps boiling 30min, Then with the filter-cloth filtering of 400 mesh, deionized water is added again in quality and the ratio of volume proportion (grams per milliliter) 1:10 in the dregs of a decoction Secondary to boil and keep boiling 30min, filtering merges filtrate twice and extracting solution is distinguished constant volume, makes puncture vine and water ratio 1:10 (i.e. 10ml extracting solution is made in 1g puncture vine altogether) obtains the puncture vine extracting solution that mass concentration is 10%.
Example given above is to realize the present invention preferably example, and the present invention is not limited to the above embodiments.This field Technical staff's technical solution according to the present invention technical characteristic any nonessential addition, the replacement made, belong to this The protection scope of invention.

Claims (10)

1. a kind of probiotic formulation goat milk powder, which is characterized in that by mass fraction by 5600-6400 parts of fresh sheep creams or 700-800 The full-cream goat milk powder of part, 108-249 parts of desalted whey powders, 30-50 parts of whey protein concentrate, 20-40 parts of stachyoses, 0.3-1 parts of dimensions Raw C, 0.5-1 parts of sheep cream throw type leavens of element and 0.005-0.01 parts of probiotics bacterial powder compositions.
2. a kind of preparation method of probiotic formulation goat milk powder, which comprises the following steps:
Step 1, prepared by sheep cream throw type leaven: lactobacillus bulgaricus powder and streptococcus thermophilus bacterium powder being mixed, its work is made The ratio between bacterium number is that 1:1 obtains sheep cream throw type leaven；
Step 2, prepared by prebiotics goat milk powder: 108-249 parts of desalination creams are added into 5600-6400 parts of fresh goat milk by mass fraction Clear powder, 30-50 parts of whey protein concentrate, 20-40 parts of stachyoses and 0.3-1 parts of vitamin Cs, stir evenly；It is concentrated into solid content Content is the concentrated milk of 46-53%, and concentrated milk spray drying obtains prebiotics goat milk powder；Or in mass ratio to 700-800 parts 108-249 parts of desalted whey powders of addition, 30-50 parts of whey protein concentrate, 20-40 parts of stachyoses and 0.3-1 parts in full-cream goat milk Vitamin C is uniformly mixed, and obtains prebiotics goat milk powder；
Step 3, prepared by probiotic formulation goat milk powder: 0.5-1 parts of sheep cream throw type leavens are added into prebiotics goat milk powder, with And 0.005-0.01 parts of probiotics bacterial powder are to get probiotic formulation goat milk powder.
3. a kind of preparation method of probiotic formulation goat milk powder according to claim 2, which is characterized in that the step The preparation of lactobacillus bulgaricus powder in 1: 0.3% is added in the Longstamen Onion Bulb extracting solution for being 10% to mass concentration by mass volume ratio Casein hydrolysate, 0.15% sodium acetate and 0.25% Triammonium citrate, sterilize cooling culture medium；By volume to culture medium The lactobacillus bulgaricus that middle access 3% has activated, in 37 DEG C of constant temperature incubation 20h, refrigerated centrifuge obtains lactobacillus bulgaricus Then bacterium mud adds the compound freeze drying protectant of 1.2 times of lactobacillus bulgaricus bacterium mud weight, is subsequently placed in 10 DEG C of cold treatments Then 1.5h uses vacuum freeze drier freeze-drying for 24 hours to get viable count for 3.6 × 1011CFU/g lactobacillus bulgaricus bacterium Powder.
4. a kind of preparation method of probiotic formulation goat milk powder according to claim 2, which is characterized in that the step The preparation of streptococcus thermophilus bacterium powder in 1: 1.5% Portugal is added in the puncture vine extracting solution for being 10% to mass concentration by mass volume ratio Grape sugar, 0.25% soy peptone, 1% casein hydrolysate, 0.0015% glutamic acid, 0.2%KH2PO4, 0.05% Tween-80 Culture medium is obtained, pH is adjusted to 6.8；The streptococcus thermophilus activated is accessed into culture medium by volume, in 42 DEG C of constant temperature incubations 9h, refrigerated centrifuge obtain streptococcus thermophilus bacterium mud, 2 times of bacterium mud weight of compound freeze drying protectant of addition, be subsequently placed in 12 DEG C it is cold 1.5h is handled, then using vacuum freeze drier freeze-drying for 24 hours to get streptococcus thermophilus bacterium powder, viable count is 2.4 × 1011CFU/g。
5. a kind of preparation method of probiotic formulation goat milk powder according to claim 2, which is characterized in that the step The preparation of probiotics bacterial powder in 2: by mass volume ratio to mass concentration be 10% Longstamen Onion Bulb extracting solution in add 1.2% lactose, Culture medium, medium sterilization is made in 0.5% peptone, 0.25% xylo-oligosaccharide, 0.20% sodium acetate, 0.22% Triammonium citrate The probiotics activated is cooled down and is respectively connected to, for 24 hours in 37 DEG C of constant temperature incubations, refrigerated centrifuge obtains probiotics bacterium mud, addition benefit Raw 1.5 times of bacterium bacterium mud weight of compound freeze drying protectant, is placed in 10 DEG C of cold treatment 1.5h, is lyophilized using vacuum freeze drier For 24 hours to get probiotics bacterial powder.
6. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 3 or 5, which is characterized in that described The preparation of Longstamen Onion Bulb extracting solution: taking the Longstamen Onion Bulb of certain mass, soaks in water in the ratio of quality and volume proportion (grams per milliliter) 1:10 20-30min is boiled and boiling 30min is maintained to obtain filtrate with 400 mesh filter-cloth filterings；Filter residue presses quality and volume again The ratio of proportion (grams per milliliter) 1:10 is added distilled water heating and boils and maintain 20min, filters；Merge filtrate twice and constant volume, Obtain the Longstamen Onion Bulb extracting solution that mass concentration is 10%.
7. a kind of preparation method of probiotic formulation goat milk powder according to claim 3, which is characterized in that described is prebiotic The preparation of bacterium bacterium powder, when cultivating two qi Bifidobacteriums, also addition is passed through by mass volume ratio 0.05% after medium sterilization is cooling The cysteine hydrochloride of 0.22 μm of membrane filtration degerming.
8. a kind of preparation method of lactic acid bacteria formula goat milk powder according to claim 4, which is characterized in that the puncture vine The preparation of extracting solution: it takes a certain amount of puncture vine to crush, makes its granularity 20-60 mesh；By quality and volume proportion (grams per milliliter) 1: Deionized water is added in 10 ratio, boils and keeps boiling 30min, with the filter-cloth filtering of 400 mesh；Filter residue is matched by quality and volume Ratio than (grams per milliliter) 1:10 is added deionized water and boils again and keep boiling 30min, filtering；Merge twice that filtrate is simultaneously Constant volume obtains puncture vine extracting solution.
9. a kind of preparation method of probiotic formulation goat milk powder according to claim 2, which is characterized in that the step The viable count of probiotics is 1.25 × 10 in probiotic formulation goat milk powder made from 26CFU/g-3.5×107CFU/g。
10. a kind of preparation method of probiotic formulation goat milk powder according to claim 2, which is characterized in that the step Rapid 1 spray drying condition are as follows: inlet air temperature is 190 DEG C, and leaving air temp is 90 DEG C, and air pressure is 1 atmospheric pressure, hot-air Flow velocity is 0.25m3/min。
CN201811620591.7A 2018-12-28 2018-12-28 A kind of probiotic formulation goat milk powder and preparation method thereof Pending CN109527096A (en)

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CN110810515A (en) * 2019-11-25 2020-02-21 海南国健高科技乳业有限公司 Formula milk powder for pregnant and lying-in women and preparation method thereof
CN111011867A (en) * 2019-12-25 2020-04-17 光明乳业股份有限公司 Probiotic embedded particle and preparation method thereof
CN112640958A (en) * 2020-12-25 2021-04-13 石家庄君乐宝乳业有限公司 Nutritional goat milk powder suitable for diarrhea children and preparation method and application thereof
CN113796421A (en) * 2021-08-13 2021-12-17 盛锦合生物科技(江苏)有限公司 Probiotic formula milk powder and preparation method thereof
CN114794245A (en) * 2022-05-13 2022-07-29 扬州市扬大康源乳业有限公司 Probiotic milk tablet and preparation method thereof
CN115606727A (en) * 2022-09-08 2023-01-17 陕西师范大学 Functional sugar and goat whey protein compounded microecological preparation, preparation method and application

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CN110810515A (en) * 2019-11-25 2020-02-21 海南国健高科技乳业有限公司 Formula milk powder for pregnant and lying-in women and preparation method thereof
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CN115606727B (en) * 2022-09-08 2023-09-01 陕西师范大学 Microecological preparation compounded by functional sugar and sheep whey protein, preparation method and application

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Application publication date: 20190329