CN102754690A - Synbiotics goat milk slices and method for preparing same - Google Patents
Synbiotics goat milk slices and method for preparing same Download PDFInfo
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- CN102754690A CN102754690A CN2012102375578A CN201210237557A CN102754690A CN 102754690 A CN102754690 A CN 102754690A CN 2012102375578 A CN2012102375578 A CN 2012102375578A CN 201210237557 A CN201210237557 A CN 201210237557A CN 102754690 A CN102754690 A CN 102754690A
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Abstract
The invention discloses a synbiotics goat milk slice and a method for preparing the same. The method comprises the following steps of: firstly, inoculating activated probiotics to the fermentation tank of an MRS culture medium for cultivation, performing centrifuging to prepare probiotics mud, adding a composite freeze-drying protective agent to the probiotics mud and carrying out vacuum freeze-drying to obtain freeze-dried probiotics powder; carrying out decompression concentration, spray drying and cooling till the temperature reaches the room temperature after goat milk is purified and standardized to obtain goat milk powder; evenly mixing the goat milk powder, the probiotics, cane sugar, glucose, synbiotics and magnesium stearate, and then preparing the synbiotics goat milk slices by means of a single-punch tablet press. The synbiotics goat milk slice is added with the probiotics and the synbiotics; therefore, the physiological bacterial activity of the probiotics can be brought into play; and the quantity of the bacteria can be quickly increased selectively, so that the effect of the probiotics is more obvious and longer; and the probiotics and the synbiotics are combined to resist diseases together and to maintain micro-ecological balance of the organism.
Description
Technical field
The invention belongs to field of health care food, the present invention relates to a kind of synbiotic goat milk sheet and preparation method thereof.
Background technology
Goat milk is one of human main newborn source, the nearly edible goat milk of population about half in the whole world.The main composition of goat milk and milk is close, and some nutrient exceeds much than milk.Contain some particular matters that milk does not possess,, do not contain the casein of allergin α-S1 that milk causes like EGF EGF, immunoglobulin (Ig).Iron in the milk and calcium can have influence on wherein a kind of absorption of element because interaction is arranged.And iron in the Goat Milk and calcium do not have this interaction, so bioavilability is higher than milk.Goat milk fat globule little (be merely milk 1/3); And in the Goat Milk, the short chain unsaturated fatty acid content is abundant, especially medium chain fatty acid content is high, human body is prone to absorb; But and enhancing body immunity; Regulating the human body nutrient balance, improve sleep quality, is infant, pregnant woman, old man and patient's excellent health products.
Prebiotics can be used as a kind of meal supplement conditioning agent, optionally increases the quantity of probio, optimizes the health that flora comes the balance human body; Some compound sugar in the prebiotics also have the function of dietary fiber; Can increase retentiveness and the capacity of stool, thereby be easy to discharge, play the effect of " street cleaner in the intestines "; Constipation, diarrhoea are played bidirectional modulation, anion in the also adsorbable enteron aisle, bile acid and effectively reduce blood fat and cholesterol.Main functional oligose is an oligomeric maltose, galactooligosaccharide, FOS, isomaltose, milk ketose, xylo-oligosaccharide, stachyose, raffinose, soyabean oligosaccharides, synanthrin etc.
Symphysis unit is called Synbiotics again, is meant the combination preparation of probio and prebiotics.It both can bring into play the physiological bacterial activity of probio, optionally increased the quantity of this bacterium again fast, made probiotic action significantly lasting, resisted disease jointly, safeguarded the microecological balance of body.
Patent (CN101449708) has been announced a kind of baby milk powder that adds symphysis unit and preparation method thereof; Its invention relates to and milk powder; Do not relate to goat milk powder, and therefore goat milk many advantages of comparing with milk are applied to have huge development potentiality in the goat milk powder with symphysis unit.
Summary of the invention
The object of the present invention is to provide a kind of synbiotic goat milk sheet and preparation method thereof that contains.
For achieving the above object, of the present inventionly contain the goat milk powder that synbiotic goat milk tablet quality percentage comprises 53%-78.9%, the freeze-drying probiotics bacterial powder of 0.1%-1%, the sucrose of 5%-7%, the glucose of 5%-8%, 10%-30% prebiotics and 1% dolomol.
Described prebiotics is the mixture of two or more arbitrary proportion in stachyose, trehalose, chitosan oligomer, isomaltoketose, the oligomeric dragon gallbladder sugar.
Described freeze-drying probiotics bacterial powder is the mixture of probiotics bacterial mud and compound freeze drying protectant;
The mixed of the compound freeze drying protectant of probiotics bacterial mud and compound freeze drying protectant every g probio mud and 1mL wherein;
It is 1.0-3.0 * 10 that said probiotics bacterial mud adopts viable count
11Lactobacillus acidophilus, viable count be 1.0-3.0 * 10
10Lactobacillus casei, viable count be 1.0-3.0 * 10
11Bifidobacterium, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum, viable count be 1.0-3.0 * 10
10Lactobacillus rhamnosus, viable count be 1.0-3.0 * 10
11Lactobacillus reuteri or viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of lactobacillus bulgaricus;
Compound freeze drying protectant comprises the skimmed milk power of 18%-50% by mass percentage, the trehalose of 8%-20%, the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer.
Described Bifidobacterium is bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium.
Preparation method of the present invention may further comprise the steps:
1) at first, the probio of getting after the activation is inserted the fermentation tank culture 18h of 10L MRS culture medium with 5% inoculum concentration, whenever in incubation stirs once at a distance from 3-4h; Rotating speed 60 commentaries on classics/min; Mixing time 2min then, processes probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min; In probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then; It is even to utilize the vortex mixed instrument to be mixed, and at-40～-20 ℃ of pre-freeze 12～24h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed;
2) with sheep breast through purify and standardization after, decompression concentrates and makes solid content reach 42～50% to obtain raw material sheep breast, raw material sheep breast after through the spray-drying cool to room temperature 40～80 purposes sieve goat milk powder;
3) by mass percentage with the goat milk powder of 53%-78.9%; The probio of 0.1%-1%; The sucrose of 5%-7%, the glucose of 5%-8%, 10%-30% prebiotics and 1% dolomol mix; Utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
It is 1.0-3.0 * 10 that said probiotics bacterial mud adopts viable count
11Lactobacillus acidophilus, viable count be 1.0-3.0 * 10
10Lactobacillus casei, viable count be 1.0-3.0 * 10
11Bifidobacterium, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum, viable count be 1.0-3.0 * 10
10Lactobacillus rhamnosus, viable count be 1.0-3.0 * 10
11Lactobacillus reuteri or viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of lactobacillus bulgaricus;
Described prebiotics is the mixture of two or more arbitrary proportion in stachyose, trehalose, chitosan oligomer, isomaltoketose, the oligomeric dragon gallbladder sugar.
The activation process of described probio is following: lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri or lactobacillus bulgaricus are adopted MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; 0.05% the cysteine hydrochloride that bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium is added culture volume in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; Transfer with 5% inoculum concentration at every turn; All under 37 ℃ of condition of culture, cultivate 20-24h wherein preceding twice; Under 37 ℃ of condition of culture, cultivate 18h for the third time, carry out microscopy then, survey the OD value, confirm behind the assorted bacterium of nothing activation for the third time to be obtained the activation probio.
Described Bifidobacterium is bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium.
Described spray-drying EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃.
It is 5～20 commentaries on classics/min that rotating speed is adopted in described mixing, and power of motor is the three-dimensional motion mixer of 0.5Kw;
The maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 8～14mm; In the sheet 0.4～0.6g; Hardness is at 4～6N.
The effect of invention
1) main component is a goat milk powder among the present invention; Because Goat Milk is rich in high quality protein matter and aliphatic acid and several mineral materials such as albumin that health is played an important role, globulin, bad syndrome patient has good health care and therapeutic action to nutrient absorption.Goat milk fat globule little (be merely milk 1/3), and be rich in be beneficial to that body directly utilizes in, the short chain unrighted acid, absorptivity is very high.The EGF that contains in the goat milk (EGF) when promoting epithelial cell growth, has repair to mucomembranous epithelial cells such as stomach, tracheae and skins; Can urge digestion again can tranquilizing and allaying excitement (because of containing cyclic nucleotide); But and enhancing body immunity, regulate the human body nutrient balance, improve sleep quality; The comfort cushioning characteristic of its meta-alkalescence also has the effect that promotes rehabilitation to diseases such as ephritis, tuberculosis and asthma, is infant, pregnant woman, old man and patient's excellent health products.
2) added compound sugar in the present invention as prebiotics; Especially functional oligose; Owing to compare with common compound sugar; Functional oligose has special physiological properties: functional oligose can promote Bifidobacterium propagation, because Bifidobacterium is that a kind of endotoxin that neither produces unique in human intestine's flora does not produce exotoxin again, the beneficial microbe with many physiological functions of no pathogenicity.Human body there are many health-care effects, as improve vitamin metabolism, prevent bowel dysfunction, suppress the growth of harmful bacterium and pathogenic bacteria in the enteron aisle, play anti-ageing, anti-cancer and liver-protective effect etc.The energy of functional oligose is low or be zero energy, can significantly reduce diabetes patient, obesity patient's quantity.The edible cavity disease that can not cause carious tooth of child.Compound sugar belongs to water-soluble dietary fiber, can prevent constipation after eating, and reduces cholesterol in addition, enhancing body immunocompetence, functions such as opposing tumour.
3) add symphysis unit among the present invention, both can bring into play the physiological bacterial activity of probio, optionally increase the quantity of this bacterium again fast, make probiotic action significantly lasting, resist disease jointly, safeguard the microecological balance of body.
The specific embodiment
Further specify the present invention below in conjunction with embodiment:
Embodiment 1:
1) at first, lactobacillus acidophilus, Lactobacillus plantarum are adopted MRS fluid nutrient medium activation three times respectively under 37 ℃ of condition of culture; Get lactobacillus acidophilus and Lactobacillus plantarum after the activation insert 10L MRS culture medium with 5% inoculum concentration fermentation tank culture 18h; Every separated 3h stirs once rotating speed 60 commentaries on classics/min, mixing time 2min in incubation; Then; Process probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, add the compound freeze drying protectant of 1mL then by every g probio mud, it is even to utilize the vortex mixed instrument to be mixed; At-40 ℃ of pre-freeze 12h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer are formed;
2) sheep is newborn after purification and standardization; Decompression concentrates and to make solid content reach 42% to obtain raw material sheep breast; Raw material sheep breast through mistake 60 purposes behind spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) cool to room temperature sieve goat milk powder;
3) adopting rotating speed is 5 commentaries on classics/min; Power of motor is the goat milk powder of the three-dimensional motion mixer of 0.5Kw with mass percent 53%, 1% probio, 7% sucrose; 8% glucose; 30% prebiotics and 1% dolomol mix, and utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 14mm; In the sheet 0.6g; Hardness is at 4～6N, and the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
Described prebiotics is the mixture of stachyose and trehalose.
Embodiment 2:
1) at first, lactobacillus acidophilus and Lactobacillus rhamnosus are adopted MRS fluid nutrient medium activation three times respectively under 37 ℃ of condition of culture; Get lactobacillus acidophilus and Lactobacillus rhamnosus after the activation are inserted 10L MRS culture medium with 5% inoculum concentration fermentation tank culture 18h; Every separated 4h stirs once rotating speed 60 commentaries on classics/min, mixing time 2min in incubation; Then; Process probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, add the compound freeze drying protectant of 1mL then by every g probio mud, it is even to utilize the vortex mixed instrument to be mixed; At-30 ℃ of pre-freeze 18h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 50% skimmed milk power, 8% trehalose, and 1% sodium hydrogen phosphate, 1% lactose and 40% phosphate buffer are formed;
2) sheep is newborn after purification and standardization; Decompression concentrates and to make solid content reach 45% to obtain raw material sheep breast; Raw material sheep breast through mistake 80 purposes behind spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) cool to room temperature sieve goat milk powder;
3) adopting rotating speed is 10 commentaries on classics/min; Power of motor is the goat milk powder of the three-dimensional motion mixer of 0.5Kw with mass percent 78.9%; 0.1% probio, the glucose of 5% sucrose 5%, 10% prebiotics and 1% dolomol mix; Utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 8mm; In the sheet 0.4g; Hardness is at 4～6N, and the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
Described prebiotics is the mixture of chitosan oligomer, isomaltoketose and oligomeric dragon gallbladder sugar.
Embodiment 3:
1) at first, lactobacillus acidophilus is adopted MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; 0.05% the cysteine hydrochloride that bifidobacterium infantis is added culture volume in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; At every turn with 5% inoculum concentration switching, wherein preceding twice all cultivation 20h under 37 ℃ of condition of culture, cultivation 18h under 37 ℃ of condition of culture for the third time; Carry out microscopy then, survey the OD value; After confirm having assorted bacterium activation is not for the third time obtained the activation bifidobacterium infantis, lactobacillus acidophilus and the bifidobacterium infantis got after the activation insert the fermentation tank culture 18h of 10L MRS culture medium (adding the cysteine hydrochloride of 5g in the MRS culture medium) with 5% inoculum concentration, and every separated 4h stirring once in incubation; Rotating speed 60 commentaries on classics/min; Mixing time 2min then, processes probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min; In probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then; It is even to utilize the vortex mixed instrument to be mixed, and at-25 ℃ of pre-freeze 21h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 30% skimmed milk power, 15% trehalose, and 1.5% sodium hydrogen phosphate, 5% lactose and 48.5% phosphate buffer are formed;
2) sheep is newborn after purification and standardization; Decompression concentrates and to make solid content reach 50% to obtain raw material sheep breast; Raw material sheep breast through mistake 40 purposes behind spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) cool to room temperature sieve goat milk powder;
3) adopting rotating speed is 15 commentaries on classics/min; Power of motor is the goat milk powder of the three-dimensional motion mixer of 0.5Kw with mass percent 60%, 0.5% probio, 6.5% sucrose; 7% glucose; 25% prebiotics and 1% dolomol mix, and utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 10mm; In the sheet 0.5g; Hardness is at 4～6N, and the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
Described prebiotics is the mixture of oligomeric dragon gallbladder sugar, chitosan oligomer, trehalose.
Embodiment 4:
1) at first, Lactobacillus plantarum is adopted MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; 0.05% the cysteine hydrochloride that bifidobacterium breve is added culture volume in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; At every turn with 5% inoculum concentration switching, wherein preceding twice all cultivation 22h under 37 ℃ of condition of culture, cultivation 18h under 37 ℃ of condition of culture for the third time; Carry out microscopy then, survey the OD value; After confirm having assorted bacterium activation is not for the third time obtained the activation probio, the probio of getting after the activation is inserted the fermentation tank culture 18h of 10L MRS culture medium (adding the cysteine hydrochloride of 5g in the MRS culture medium) with 5% inoculum concentration, in incubation every at a distance from the 3h stirring once; Rotating speed 60 commentaries on classics/min; Mixing time 2min then, processes probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min; In probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then; It is even to utilize the vortex mixed instrument to be mixed, and at-35 ℃ of pre-freeze 15h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 40% skimmed milk power, 12% trehalose, and 1% sodium hydrogen phosphate, 3% lactose and 44% phosphate buffer are formed;
2) sheep is newborn after purification and standardization; Decompression concentrates and to make solid content reach 46% to obtain raw material sheep breast; Raw material sheep breast through mistake 50 purposes behind spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) cool to room temperature sieve goat milk powder;
3) adopting rotating speed is 20 commentaries on classics/min; Power of motor is the goat milk powder of the three-dimensional motion mixer of 0.5Kw with mass percent 70%, 0.8% probio, 6% sucrose; 6.2% glucose; 16% prebiotics and 1% dolomol mix, and utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 12mm; In the sheet 0.55g; Hardness is at 4～6N, and the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
Described prebiotics is the mixture of stachyose, trehalose, chitosan oligomer and isomaltoketose.
Embodiment 5:
1) at first, Lactobacillus rhamnosus is adopted MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; 0.05% the cysteine hydrochloride that blue and green Bifidobacterium is added culture volume in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; At every turn with 5% inoculum concentration switching, wherein preceding twice all cultivation 24h under 37 ℃ of condition of culture, cultivation 18h under 37 ℃ of condition of culture for the third time; Carry out microscopy then, survey the OD value; After confirm having assorted bacterium activation is not for the third time obtained the activation probio, the probio of getting after the activation is inserted the fermentation tank culture 18h of 10L MRS culture medium (adding the cysteine hydrochloride of 5g in the MRS culture medium) with 5% inoculum concentration, in incubation every at a distance from the 4h stirring once; Rotating speed 60 commentaries on classics/min; Mixing time 2min then, processes probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min; In probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then; It is even to utilize the vortex mixed instrument to be mixed, and at-20 ℃ of pre-freeze 24h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 35% skimmed milk power, 15% trehalose, and 1% sodium hydrogen phosphate, 8% lactose and 41% phosphate buffer are formed;
2) sheep is newborn after purification and standardization; Decompression concentrates and to make solid content reach 48% to obtain raw material sheep breast; Raw material sheep breast through mistake 70 purposes behind spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) cool to room temperature sieve goat milk powder;
3) adopting rotating speed is 18 commentaries on classics/min; Power of motor is the goat milk powder of the three-dimensional motion mixer of 0.5Kw with mass percent 63%, 1% probio, 7% sucrose; 8% glucose; 20% prebiotics and 1% dolomol mix, and utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 10mm; In the sheet 0.5g; Hardness is at 4～6N, and the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
Described prebiotics is the mixture of stachyose, oligomeric dragon gallbladder sugar, isomaltoketose and trehalose.
Claims (10)
1. synbiotic goat milk sheet is characterized in that: comprise the goat milk powder of 53%-78.9% by mass percentage, the freeze-drying probiotics bacterial powder of 0.1%-1%, the sucrose of 5%-7%, the glucose of 5%-8%, 10%-30% prebiotics and 1% dolomol.
2. synbiotic goat milk sheet according to claim 1 is characterized in that: described prebiotics is the mixture of two or more arbitrary proportion in stachyose, trehalose, chitosan oligomer, isomaltoketose, the oligomeric dragon gallbladder sugar.
3. synbiotic goat milk sheet according to claim 1 is characterized in that:
Described freeze-drying probiotics bacterial powder is the mixture of probiotics bacterial mud and compound freeze drying protectant;
The mixed of the compound freeze drying protectant of probiotics bacterial mud and compound freeze drying protectant every g probio mud and 1mL wherein;
It is 1.0-3.0 * 10 that said probiotics bacterial mud adopts viable count
11Lactobacillus acidophilus, viable count be 1.0-3.0 * 10
10Lactobacillus casei, viable count be 1.0-3.0 * 10
11Bifidobacterium, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum, viable count be 1.0-3.0 * 10
10Lactobacillus rhamnosus, viable count be 1.0-3.0 * 10
11Lactobacillus reuteri or viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of lactobacillus bulgaricus;
Compound freeze drying protectant comprises the skimmed milk power of 18%-50% by mass percentage, the trehalose of 8%-20%, the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer.
4. synbiotic goat milk sheet as claimed in claim 3, it is characterized in that: described Bifidobacterium is bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium.
5. the preparation method of a synbiotic goat milk sheet is characterized in that may further comprise the steps:
1) at first, the probio of getting after the activation is inserted the fermentation tank culture 18h of 10L MRS culture medium with 5% inoculum concentration, whenever in incubation stirs once at a distance from 3-4h; Rotating speed 60 commentaries on classics/min; Mixing time 2min then, processes probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min; In probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then; It is even to utilize the vortex mixed instrument to be mixed, and at-40～-20 ℃ of pre-freeze 12～24h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed;
2) with sheep breast through purify and standardization after, decompression concentrates and makes solid content reach 42～50% to obtain raw material sheep breast, raw material sheep breast after through the spray-drying cool to room temperature 40～80 purposes sieve goat milk powder;
3) by mass percentage with the goat milk powder of 53%-78.9%; The probio of 0.1%-1%; The sucrose of 5%-7%, the glucose of 5%-8%, 10%-30% prebiotics and 1% dolomol mix; Utilize one-shot formula compressing tablet mechanism synbiotic goat milk sheet then, the unit viable count of synbiotic goat milk sheet reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9Cfu/g.
6. the preparation method of synbiotic goat milk sheet according to claim 5 is characterized in that: it is 1.0-3.0 * 10 that said probiotics bacterial mud adopts viable count
11Lactobacillus acidophilus, viable count be 1.0-3.0 * 10
10Lactobacillus casei, viable count be 1.0-3.0 * 10
11Bifidobacterium, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum, viable count be 1.0-3.0 * 10
10Lactobacillus rhamnosus, viable count be 1.0-3.0 * 10
11Lactobacillus reuteri or viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of lactobacillus bulgaricus;
Described prebiotics is the mixture of two or more arbitrary proportion in stachyose, trehalose, chitosan oligomer, isomaltoketose, the oligomeric dragon gallbladder sugar.
7. the preparation method of synbiotic goat milk sheet according to claim 5 is characterized in that: the activation process of described probio is following: lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri or lactobacillus bulgaricus are adopted MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; 0.05% the cysteine hydrochloride that bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium is added culture volume in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture; Transfer with 5% inoculum concentration at every turn; All under 37 ℃ of condition of culture, cultivate 20-24h wherein preceding twice; Under 37 ℃ of condition of culture, cultivate 18h for the third time, carry out microscopy then, survey the OD value, confirm behind the assorted bacterium of nothing activation for the third time to be obtained the activation probio.
8. the preparation method of synbiotic goat milk sheet according to claim 7 is characterized in that: described Bifidobacterium is bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium.
9. the preparation method of synbiotic goat milk sheet according to claim 7 is characterized in that: described spray-drying EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃.
10. the preparation method of synbiotic goat milk sheet according to claim 7 is characterized in that: it is 5～20 commentaries on classics/min that rotating speed is adopted in described mixing, and power of motor is the three-dimensional motion mixer of 0.5Kw;
The maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 8～14mm; In the sheet 0.4～0.6g; Hardness is at 4～6N.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081996A (en) * | 2013-01-22 | 2013-05-08 | 昆山佰生优生物科技有限公司 | Orally taken yoghurt starter particles and preparation method thereof |
| CN103392818A (en) * | 2013-07-30 | 2013-11-20 | 李卫平 | Functional compressed dairy product and preparation method thereof |
| CN103651804A (en) * | 2013-12-18 | 2014-03-26 | 南昌大学 | Prebiotic and probiotic added milk tablet based on homology of medicine and food |
| CN103918795A (en) * | 2014-04-10 | 2014-07-16 | 陕西科技大学 | Tea polyphenols probiotics goat milk tablet and preparation method thereof |
| CN103918792A (en) * | 2014-04-10 | 2014-07-16 | 陕西科技大学 | Xylitol probiotics goat milk tablets and preparation method thereof |
| CN104366486A (en) * | 2014-12-10 | 2015-02-25 | 孙伟 | Formula and preparation method of quintuplet probiotic chewable tablet |
| CN107518073A (en) * | 2017-08-10 | 2017-12-29 | 山东阳春羊奶乳业有限公司 | A kind of probiotics goat milk piece and preparation method thereof |
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| CN108782758A (en) * | 2018-06-22 | 2018-11-13 | 陕西师范大学 | A kind of fermented type synbiotic goat milk powder and preparation method thereof |
| CN108935709A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk piece and preparation method thereof |
| CN108991115A (en) * | 2018-06-22 | 2018-12-14 | 陕西师范大学 | A kind of fermented type synbiotic goat milk piece and preparation method thereof |
| CN110200119A (en) * | 2019-06-21 | 2019-09-06 | 西安优乐贝孕婴用品有限公司 | A kind of compound lactobacillus goat milk piece pressed candy and preparation method thereof |
| CN111194786A (en) * | 2020-03-02 | 2020-05-26 | 兰州大学 | Calcium milk tablet containing dangshen oligosaccharide and preparation method thereof |
| CN116396904A (en) * | 2023-04-04 | 2023-07-07 | 陕西科技大学 | Preparation of probiotics powder and application of probiotics powder in goat milk powder |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099513A (en) * | 2007-07-17 | 2008-01-09 | 广东省农业科学院蚕业与农产品加工研究所 | Cordyceps sinensis dried milk slices and producing method thereof |
| CN101810222A (en) * | 2010-04-23 | 2010-08-25 | 陕西农产品加工技术研究院 | Synbiotic goat milk powder |
| CN101810220A (en) * | 2010-04-23 | 2010-08-25 | 陕西农产品加工技术研究院 | Preparation method of synbiotic goat milk powder |
| CN102125105A (en) * | 2010-12-14 | 2011-07-20 | 内蒙古伊利实业集团股份有限公司 | New-prebiotics-added milk slice and preparation method thereof |
| CN102239912A (en) * | 2007-12-12 | 2011-11-16 | 章传华 | Red beet lactic acid bacteria milk series foods |
| CN102450331A (en) * | 2010-10-25 | 2012-05-16 | 王世义 | Composite active probiotics milk powder candy |
-
2012
- 2012-07-10 CN CN2012102375578A patent/CN102754690A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099513A (en) * | 2007-07-17 | 2008-01-09 | 广东省农业科学院蚕业与农产品加工研究所 | Cordyceps sinensis dried milk slices and producing method thereof |
| CN102239912A (en) * | 2007-12-12 | 2011-11-16 | 章传华 | Red beet lactic acid bacteria milk series foods |
| CN101810222A (en) * | 2010-04-23 | 2010-08-25 | 陕西农产品加工技术研究院 | Synbiotic goat milk powder |
| CN101810220A (en) * | 2010-04-23 | 2010-08-25 | 陕西农产品加工技术研究院 | Preparation method of synbiotic goat milk powder |
| CN102450331A (en) * | 2010-10-25 | 2012-05-16 | 王世义 | Composite active probiotics milk powder candy |
| CN102125105A (en) * | 2010-12-14 | 2011-07-20 | 内蒙古伊利实业集团股份有限公司 | New-prebiotics-added milk slice and preparation method thereof |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081996A (en) * | 2013-01-22 | 2013-05-08 | 昆山佰生优生物科技有限公司 | Orally taken yoghurt starter particles and preparation method thereof |
| CN103081996B (en) * | 2013-01-22 | 2016-12-28 | 昆山佰生优生物科技有限公司 | Orally available ferment agent for Yoghourt granule and preparation method thereof |
| CN103392818A (en) * | 2013-07-30 | 2013-11-20 | 李卫平 | Functional compressed dairy product and preparation method thereof |
| CN103651804A (en) * | 2013-12-18 | 2014-03-26 | 南昌大学 | Prebiotic and probiotic added milk tablet based on homology of medicine and food |
| CN103918795A (en) * | 2014-04-10 | 2014-07-16 | 陕西科技大学 | Tea polyphenols probiotics goat milk tablet and preparation method thereof |
| CN103918792A (en) * | 2014-04-10 | 2014-07-16 | 陕西科技大学 | Xylitol probiotics goat milk tablets and preparation method thereof |
| CN104366486A (en) * | 2014-12-10 | 2015-02-25 | 孙伟 | Formula and preparation method of quintuplet probiotic chewable tablet |
| CN107518073A (en) * | 2017-08-10 | 2017-12-29 | 山东阳春羊奶乳业有限公司 | A kind of probiotics goat milk piece and preparation method thereof |
| CN108295097A (en) * | 2018-02-02 | 2018-07-20 | 云南中京国建投资有限公司 | The symphysis unit composition and its preparation of a kind of reducing blood lipid and application |
| CN108782758A (en) * | 2018-06-22 | 2018-11-13 | 陕西师范大学 | A kind of fermented type synbiotic goat milk powder and preparation method thereof |
| CN108991115A (en) * | 2018-06-22 | 2018-12-14 | 陕西师范大学 | A kind of fermented type synbiotic goat milk piece and preparation method thereof |
| CN108782758B (en) * | 2018-06-22 | 2022-03-08 | 浙江忠梦昌健康科技有限公司 | Fermented synbiotic goat milk powder and preparation method thereof |
| CN108935709A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk piece and preparation method thereof |
| CN110200119A (en) * | 2019-06-21 | 2019-09-06 | 西安优乐贝孕婴用品有限公司 | A kind of compound lactobacillus goat milk piece pressed candy and preparation method thereof |
| CN111194786A (en) * | 2020-03-02 | 2020-05-26 | 兰州大学 | Calcium milk tablet containing dangshen oligosaccharide and preparation method thereof |
| CN116396904A (en) * | 2023-04-04 | 2023-07-07 | 陕西科技大学 | Preparation of probiotics powder and application of probiotics powder in goat milk powder |
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