CN102742654B - Beautification probiotic ewe milk tablet and preparation method thereof - Google Patents
Beautification probiotic ewe milk tablet and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a beautification probiotic ewe milk tablet and a preparation method thereof, wherein the beautification probiotic ewe milk tablet comprises the following components in mass percentage: 53-78.9% of ewe milk powder, 10-30% of pearl powder, grape pip, rose and burdock composite powder, 0.1-1% of freeze-dried probiotic powder, 5-7% of sucrose, 5-8% of glucose and 1% of magnesium stearate. According to the invention, beautification factors and probiotics are added into the ewe milk powder to prepare the tablet, so that the beautification probiotic ewe milk tablet has the effects of beautifying and prolonging the life after being taken.
Description
Technical field
The invention belongs to field of health care food, the present invention relates to a kind of skin maintenance probio goat milk sheet and preparation method thereof.
Background technology
Goat Milk is just published as far back as the seventies in last century the United Nations's food and agricultural organization (FAO) " goat observation " (Observations On Goat) book, wherein mention: " very early, Goat Milk has been widely regarded as and has been particularly suitable for the food that the elderly, sick people, sick convalescent people and infant use.Under many circumstances, when individuality produced allergy to milk, the use of Goat Milk not only can be removed allergic symptom, and the growth of health is grown according to normal speed ".Goat milk is existing to have many advantages for milk: 1. Goat Milk medium chain fatty acid content height, wherein aliphatic acid be divided into long, in, three kinds of short chains.Medium chain and SCFA are subjected to the attack of body fat enzyme and are digested and assimilated than long-chain is easier.In addition, medium chain fatty acid has unique mechanism of absorption, can directly be absorbed at small intestine without the digestion of pancreas enzyme and cholate, and the content of Goat Milk medium chain fatty acid is the twice of milk.2. the diameter of the little fat globule of Goat Milk fat globule diameter is more little, just has bigger surface area to attack for human intestine's lipase, and fat is decomposed faster.In general, the digestion of Goat Milk is than milk fast 20%.Though milk and Goat Milk separately the diameter of fat globule have different,, on average, the diameter of Goat Milk fat globule is more much smaller than milk.On general, the diameter of Goat Milk fat globule is the 2-2.5 micron, and milk is the 3-4.25 micron.The digestion process of little, the frangible protein of curdled milk hardness in human body that Goat Milk forms is to form curdled milk earlier under the hydrochloric acid in gastric juice effect, and the digestion that is decomposed then is absorbed at small intestine afterwards for amino acid.The soft durometer of ziega directly influences speed and the efficient that protein is digested.Main protein is beta-casein in the Goat Milk, with protein alpha-S1 casein main in the milk structural difference is arranged.It is soft more frangible than milk that the ziega that the Goat Milk beta-casein forms under one's belt has been proved to be.3. strengthen the absorption of iron and calcium.Iron in the milk and calcium can have influence on wherein a kind of absorption of element because interaction is arranged.And the iron in the Goat Milk and calcium do not have this interaction, so bioavilability is than milk height.
Probio (Probiotics), claim probiotic, regulator again, give birth to rhzomorph, viable bacteria element etc., namely refer to certain microorganism formulation or fermented product, by regulating host's mucous membrane and systemic immune function and improving enteral nutrition and colony balance, the host is produced useful physiological action, by growing the composition of a certain position of effect change host flora surely, be conducive to the single of host health effect or form clear and definite mixed microorganism thereby produce.Up to now, the probio that scientist has found can be divided into three major types substantially, comprising: 1. lactobacillus class (as lactobacillus acidophilus, Lactobacillus casei, lactobacillus bulgaricus, lactobacillus plantarum etc.); 2. Bifidobacterium class (as bifidobacterium longum, bifidobacterium adolescentis, bifidobacterium breve, bifidobacterium infantis etc.); 3. gram-positive cocci (as streptococcus fecalis, galactococcus, intermediary streptococcus etc.).Probio is as a frontier, and in recent years, the special physiological activity of probio is widely used in food, except being applied to fermented dairy product, also just progressively is applied to developing various functional foods.At present, probio is mainly used in fields such as food, functional food, dietary supplements, medicinal organism goods and feed.The food that contains profitable probliotics is referred to as probiotic food, mainly comprises the food of direct interpolation probio and through the food of probiotics fermention.Probio the most general application in field of food is still the dairy products field, comprises acidified milk, active lactic acid bacteria drink, cheese, sour cream, kefyr, fruit juice, cereal preparation, biscuit etc.With the probio bacterial classification Bifidobacterium (Bifidobateria), Bacillus acidi lactici and some streptococcus are arranged, wherein, Bacillus acidi lactici is commonly used lactobacillus acidophilus (Lactobicullus acidophilus), Lactobacillus casei (L.casei), lactobacillus bulgaricus (L.bulgaricus) etc.The probio bacterial classification that can be used for health food of China Ministry of Public Health approval at present has: lactobacillus acidophilus, Lactobacillus casei, lactobacillus reuteri, Lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum, blue and green Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus etc.
Pearl powder is a kind of traditional beauty and skin care good merchantable brand, no matter is that Orally taken and externally all has good effect.Pearl is applied to medicine in the history in existing more than 2,000 year of China as medicinal material, " book on Chinese herbal medicine is through collection ", " Haiyao Bencao, Oversea Materia Medica ", " Kaibao Bencao ", the Compendium of Material Medica of the Ming Dynasty of Song dynasty of the Tang Dynasty in the medical book " Mingyi Bielu " when three states, beam generation, 19 kinds of medical ancient books such as Leigon's Nature of Drugs in Songs of the Qing Dynasty, all the curative effect to pearl has clear and definite record.The modern Pharmacopoeia of the People's Republic of China and " Chinese medicine voluminous dictionary " all indicate: pearl has effects such as the arresting convulsion of calming the nerves, the screen that makes eye bright, detoxify and promote granulation, and modern study shows that also pearl powder is in the effect that improves body immunity, delays senility, aspects such as freckle removing and whitening, supplement calcium all have uniqueness.
It contains grape pip and enriches various polyphenoils, amino acid, vitamin and mineral matter etc.Therefore has the effect of health care, beauty treatment.Having superpower oxidation resistance from grape seed extract OPC, is 50 times of vitamin e, the energy delaying aging, prevention of arterial hardens, and the title of skin vitamin is also arranged, and is 20 times of Vitamin C, phenol anthocyanidin wherein according to fat-soluble and water miscible speciality, has whitening function.Discover that in addition grape seed extract has the effect of removing free radical, anti-oxidant, anti-prostate cancer, anti-liver neoplasm, can also resist neural damage.
Rose has strong liver and nourishing stomach, promoting blood circulation for regulating menstruation relaxes bowel, the effect of resolving stagnation for tranquilization, can chill out, balance endocrine, the gas of enriching blood, liver and stomach be had the effect of conditioning, the mood of releiving also has anti-inflammation and sterilization, eliminates tired, as to improve physique, moist skin effect.
Burdock has another name called white muscle genseng, the sticking root of mouse, black radiss, tree root dish etc., belongs to composite family Arctium biennial herb plant, is product with the fleshy root.Burdock is a kind of industrial crops, and good reputations such as " kings of vegetables ", " white muscle genseng ", " dali ginseng " are arranged.Burdock is precious, nutritious from head to foot, has multiple pharmacological effect, and the pharmacological action with burdock in " modern Chinese herbal medicine voluminous dictionary " is summarised as " antibiotic, anticancer, anti-ageing " three large effects.Be rich in the necessary mineral matter of human body and amino acid such as polysaccharide, cellulose, carrotene, polyphenol, calcium, iron, zinc, magnesium in the radix bardanae, have higher medicine-food two-purpose to be worth.Polysaccharide is by the D-fructose of the furan type mixture through the synthetic levulan of glycosidic bond dehydration, has multiple biologically active, and functions such as stomach invigorating benefit gas, diuresis, nourishing and strengthening vital, clearing heat and detoxicating, antibacterial, anti-inflammatory are arranged.Functions such as anti-curing oncoma, coronary heart disease, diabetes, colon disease and constipation are arranged simultaneously, in food, medical industry, have wide practical use.
In recent years, the research great majority for pearl powder, grape pip, rose, burdock are at cosmetics and skincare product, drink, beverage etc.
Summary of the invention
The object of the present invention is to provide a kind of beauty treatment cosmetic factor and probio are added to make tablet in the goat milk powder, can play skin maintenance after taking, skin maintenance probio goat milk sheet of effect such as promote longevity and preparation method thereof.
For achieving the above object, skin maintenance probio goat milk sheet of the present invention comprises the goat milk powder of 53%-78.9% by mass percentage, 10%-30% pearl powder, grape pip, rose and burdock composite powder, the freeze-drying probiotics bacterial powder of 0.1%-1%, 5%-7% sucrose, 5%-8% glucose and 1% dolomol;
Described pearl powder, grape pip, rose and burdock composite powder are by pearl powder: grape pip: rose: burdock is that the mass ratio mixing of 1:2:2:2 obtains.
Described freeze-drying probiotics bacterial powder is the mixture of probio and compound freeze drying protectant;
Wherein probio mixes with the ratio of the compound freeze drying protectant of 1mL with the every g probio of compound freeze drying protectant;
Probio is that viable count is 1.0-3.0 * 10
11Lactobacillus acidophilus bacterium powder, viable count be 1.0-3.0 * 10
11Bifidobacterium bacterium powder, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum bacterium powder, viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of Lactobacillus rhamnosus bacterium powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed.
Preparation method of the present invention may further comprise the steps:
1) with grape pip, rose drying to constant weight, fresh burdock is cut into the rectangular constant weight that is dried to that width is 5mm;
2) Powdered with being ground into after pearl powder, grape pip, rose, the mass ratio mixing of burdock by 1:2:2:2, cross the 40-80 mesh sieve then and get pearl powder, grape pip, rose and burdock composite powder;
3) with the sheep breast through purify and standardization after, decompression concentrates and makes solid content reach 42～50% to obtain raw material sheep breast, get goat milk powder by crossing 40～80 mesh sieves behind spray-drying and the cool to room temperature;
4) get probio after the activation is inserted 10L MRS culture medium with 5% inoculum concentration fermentation tank culture 18h, in incubation, stir once every 3-4h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-40～-20 ℃ of pre-freeze 12～24h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed;
5) by mass percentage with the goat milk powder of 53%-78.9%, 10%-30% pearl powder, grape pip, rose and burdock composite powder, the freeze-drying probiotics bacterial powder of 0.1%-1%, 5%-7% sucrose, 5%-8% glucose and 1% dolomol mix, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g.
The drying of described step 1) adopts blast drier 70 ℃ of dryings.
Described step 2) pulverize employing and meet operative norm Q/12NK4558-2006, power of motor is 1200W, and rotating speed is that the medicinal herb grinder of 2400r/min is pulverized.
Described spray-drying EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃.
Described probio is that viable count is 1.0-3.0 * 10
11Lactobacillus acidophilus bacterium powder, viable count be 1.0-3.0 * 10
11Bifidobacterium bacterium powder, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum bacterium powder, viable count be 1.0-3.0 * 10
10The mixing of one or more arbitrary proportions of Lactobacillus rhamnosus bacterium powder.
The activation process of described probio is as follows:
Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus are adopted MRS fluid nutrient medium activation three times respectively under 37 ℃ of condition of culture, Bifidobacterium is added culture volume 0.05% in the MRS fluid nutrient medium cysteine hydrochloride adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, transfer with 5% inoculum concentration at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h preceding twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, carry out microscopy then, survey the OD value, will activate for the third time behind the assorted bacterium of definite nothing and obtain activating probio.
It is 5～20 commentaries on classics/min that rotating speed is adopted in the mixing of described step 5), and power of motor is the three-dimensional motion mixer of 0.5Kw.
The maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 8～14mm; Be 0.4～0.6g in the sheet; Hardness is at 4～6N.
The effect of invention
1) main component is goat milk powder among the present invention, because Goat Milk is rich in high-quality protein and aliphatic acid and several mineral materials such as albumin that health is played an important role, globulin, bad syndrome patient has good health care and therapeutic action to nutrient absorption.Goat milk fat globule little (only be milk 1/3), and be rich in be beneficial to that body directly utilizes in, the short chain unrighted acid, absorptivity is very high.The EGF(EGF that contains in the goat milk), when promoting epithelial cell growth, mucomembranous epithelial cells such as stomach, tracheae and skin there is repair, can urge digestion again can tranquilizing and allaying excitement (because containing cyclic nucleotide), and can strengthen immunity of organisms, and regulate the human body nutrient balance, improve sleep quality, the comfort cushioning characteristic of its meta-alkalescence also has the effect that promotes rehabilitation to diseases such as ephritis, tuberculosis and asthma, is infant, pregnant woman, old man and patient's excellent health products.
2) added pearl powder in the present invention, because pearl has effects such as the arresting convulsion of calming the nerves, the screen that makes eye bright, detoxify and promote granulation, modern study shows that also pearl powder is in the effect that improves body immunity, delays senility, aspects such as freckle removing and whitening, supplement calcium all have uniqueness.
3) added grape pip in the present invention, it contains and enriches various polyphenoils, amino acid, vitamin and mineral matter etc.Therefore have health care, improve looks, have the effect of removing free radical, anti-oxidant, anti-prostate cancer, anti-liver neoplasm, can also resist neural damage.
4) added rose in the present invention, rose has strong liver and nourishing stomach, promoting blood circulation for regulating menstruation, relax bowel, the effect of resolving stagnation for tranquilization, can chill out, balance endocrine, the gas of enriching blood, liver and stomach be had the effect of conditioning, the mood of releiving also has anti-inflammation and sterilization, eliminates tired, as to improve physique, moist skin effect.
5) added burdock tea in the present invention, because burdock tea contains rich dietary fiber, can discharge vivotoxin, also can slow down the energy that food discharges, assemble in vivo thereby weaken fat, accelerate the sour speed that reduces fat, it not only reduces cholesterol, promotes health of heart, and helps defaecation, drainage, eliminate fast and the interior harmful metabolin piled up of prevention body, therefore very effective to toxin expelling, defaecation, lipopenicillinase, fat-reducing; Because burdock tea contains rich in protein, calcium, vitamin, wherein the content of carrotene is 150 times of carrot.So can replenish desired nutritional, these nutritional labelings can enhance metabolism, protein can improve body immunity, promotes the neonatal cell growth, has the antidotal function of preserving youthful looks.The contained nutritional labeling of burdock tea helps and keeps total balance of the body, regulates the bodily fuctions, anti-cancer is played an important role; Burdock tea has excellent prevention and inhibitory action etc. to three height, diabetes.
6) added probio in the present invention, probio has obvious facilitation to health, science proves that it has following effect: 1, prevent and improve constipation, diarrhoea, safeguard enteron aisle normal bacteria colony balance, suppress growth of pathogenic bacteria, pass through bidirectional modulation, prevent constipation, diarrhoea is adjusted the function of intestinal canal disorder, prevents various intestines problems.2, antitumor, from the isolated antitumor polysaccharide of Bifidobacterium enzyme is lost activity, thus the generation of pre-anti-cancer.3, the cholesterol in the control serum, a large amount of clinical trial both at home and abroad confirm, take the lactobacillary milk goods and have the human serum of minimizing cholesterol level, reduce the effect of cardiovascular disease incidence rate.1997, Gimland has proposed lactic acid bacteria at first can promote the catabolism of cholesterol by the degraded cholate, thereby reduced the viewpoint of cholesterol level.4, biological barrier effect reduces the generation of harmful substance in harmful bacterium metabolin, forms the biomembrane barrier that one deck has protective effect, stops the invasion of pernicious bacteria, accelerates the discharge of various toxin in the enteron aisle.Play the effect that improves intestinal environment and protection enteron aisle.5, promote generation and the absorption of nutriment in the enteron aisle, synthetic vitamin B can self be synthesized or promote to Bifidobacterium
1, B
2, B
6, B
12And the nutriment of needed by human such as amino acid., improve the absorption to calcium ion, realize stomach normalization.6, row's stool, the fat that purifies the blood, hypotensive, accelerate lipolysis, old loose fat in the adsorbent reduces serum cholesterol level.Thereby hypertension and artery sclerosis there is certain prevention effect.7, improve immunity, have the cytotostatic of promotion and increase the intestine immunity enhanced feature, prevent antibiotic side effect, the trigger cell mutation factor is had resistant function.Anti-ageing, promote longevity.8, improve lactose intolerance, the ferment that Bifidobacterium reduces lactose is to help improve lactose intolerance.9, other functions are as food hypersenstivity, prevention diabetes etc.Exactly because probio for the effect of human health, makes it receive much concern.
The specific embodiment
Embodiment 1:
1) with grape pip, rose drying to constant weight, it is the rectangular of 5mm that fresh burdock is cut into width, adopts blast drier to be dried to constant weight at 70 ℃ then respectively;
2) pearl powder, grape pip, rose, burdock are met operative norm Q/12NK4558-2006 by the mass ratio mixing back employing of 1:2:2:2, power of motor is 1200W, it is Powdered that rotating speed is that the medicinal herb grinder of 2400r/min is ground into, and crosses 40 mesh sieves then and get pearl powder, grape pip, rose and burdock composite powder;
3) with raw material sheep breast through purify and standardization after decompression concentrate make solid content reach 42% raw material sheep breast, raw material sheep breast is crossed the sub goat milk powder that gets of 40 mesh sieves by spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) back cool to room temperature;
4) at first, under 37 ℃ of condition of culture, adopt the activation of MRS fluid nutrient medium to obtain activating probio three times the probio lactobacillus acidophilus, get probio lactobacillus acidophilus after the activation is inserted the 10LMRS culture medium with 5% inoculum concentration fermentation tank culture 18h, in incubation, stir once every 3h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, and at-40 ℃ of pre-freeze 12h, moves to freeze drier freeze drying 24h again and obtains freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer are formed;
5) by mass percentage with 53% goat milk powder, 30% pearl powder, grape pip, rose and burdock composite powder, 1% freeze-drying probiotics bacterial powder, it is 5 commentaries on classics/min that 7% sucrose, 8% glucose and 1% dolomol adopt rotating speed, power of motor is that the three-dimensional motion mixer of 0.5Kw mixes, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g;
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter: φ 8mm; Be 0.4g in the sheet; Hardness is at 4～6N.
Embodiment 2:
1) with grape pip, rose drying to constant weight, it is the rectangular of 5mm that fresh burdock is cut into width, adopts blast drier to be dried to constant weight at 70 ℃ then respectively;
2) pearl powder, grape pip, rose, burdock are met operative norm Q/12NK4558-2006 by the mass ratio mixing back employing of 1:2:2:2, power of motor is 1200W, it is Powdered that rotating speed is that the medicinal herb grinder of 2400r/min is ground into, and crosses 60 mesh sieves then and get pearl powder, grape pip, rose and burdock composite powder;
3) with raw material sheep breast through purify and standardization after decompression concentrate make solid content reach 45% raw material sheep breast, raw material sheep breast is crossed the sub goat milk powder that gets of 60 mesh sieves by spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) back cool to room temperature;
4) at first, under 37 ℃ of condition of culture, adopt the activation of MRS fluid nutrient medium to obtain activating probio three times respectively probio lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus,
Get lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus rhamnosus after the activation inserted 10L MRS culture medium with 5% inoculum concentration fermentation tank culture 18h, in incubation, stir once every 4h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-30 ℃ of pre-freeze 18h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 50% skimmed milk power, 8% trehalose, and 1% sodium hydrogen phosphate, 1% lactose and 40% phosphate buffer are formed;
5) by mass percentage with 78.9% goat milk powder, 10% pearl powder, grape pip, rose and burdock composite powder, 0.1% freeze-drying probiotics bacterial powder, it is 8 commentaries on classics/min that 5% sucrose, 5% glucose and 1% dolomol adopt rotating speed, power of motor is that the three-dimensional motion mixer of 0.5Kw mixes, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g;
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter: φ 10mm; Be 0.5g in the sheet; Hardness is at 4～6N.
Embodiment 3:
1) with grape pip, rose drying to constant weight, it is the rectangular of 5mm that fresh burdock is cut into width, adopts blast drier to be dried to constant weight at 70 ℃ then respectively;
2) pearl powder, grape pip, rose, burdock are met operative norm Q/12NK4558-2006 by the mass ratio mixing back employing of 1:2:2:2, power of motor is 1200W, it is Powdered that rotating speed is that the medicinal herb grinder of 2400r/min is ground into, and crosses 80 mesh sieves then and get pearl powder, grape pip, rose and burdock composite powder;
3) with raw material sheep breast through purify and standardization after decompression concentrate make solid content reach 48% raw material sheep breast, raw material sheep breast is crossed the sub goat milk powder that gets of 80 mesh sieves by spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) back cool to room temperature;
4) first; the probiotic Lactobacillus acidophilus cultured at 37 ℃ conditions, using MRS liquid medium, activation times, to obtain activation probiotics; the Bifidobacterium in the MRS liquid medium, adding 0.05% medium volume cysteine hydrochloride, cultured at 37 ℃ conditions, using MRS liquid medium, activation times; each of 5% inoculum adapter; which the first two were cultured under the conditions at 37 ℃ 20h; s three times in the culture conditions of 37 ℃ for 18h; then microscopic examination, the measured OD value; determine the no bacteria after activation of the third time, to obtain activation of probiotics
The probio of getting after the activation is inserted the cysteine hydrochloride that adds 5g in the 10L MRS(MRS culture medium with 5% inoculum concentration) the fermentation tank culture 18h of culture medium, in incubation, stir once every 4h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-25 ℃ of pre-freeze 21h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 30% skimmed milk power, 15% trehalose, and 1.5% sodium hydrogen phosphate, 5% lactose and 48.5% phosphate buffer are formed;
5) by mass percentage with 60% goat milk powder, 25% pearl powder, grape pip, rose and burdock composite powder, 0.5% freeze-drying probiotics bacterial powder, it is 12 commentaries on classics/min that 6.5% sucrose, 7% glucose and 1% dolomol adopt rotating speed, power of motor is that the three-dimensional motion mixer of 0.5Kw mixes, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g;
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter: φ 12mm; Be 0.55g in the sheet; Hardness is at 4～6N.
Embodiment 4:
1) with grape pip, rose drying to constant weight, it is the rectangular of 5mm that fresh burdock is cut into width, adopts blast drier to be dried to constant weight at 70 ℃ then respectively;
2) pearl powder, grape pip, rose, burdock are met operative norm Q/12NK4558-2006 by the mass ratio mixing back employing of 1:2:2:2, power of motor is 1200W, it is Powdered that rotating speed is that the medicinal herb grinder of 2400r/min is ground into, and crosses 50 mesh sieves then and get pearl powder, grape pip, rose and burdock composite powder;
3) with raw material sheep breast through purify and standardization after decompression concentrate make solid content reach 46% raw material sheep breast, raw material sheep breast is crossed the sub goat milk powder that gets of 50 mesh sieves by spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) back cool to room temperature;
4) at first, under 37 ℃ of condition of culture, adopt the activation of MRS fluid nutrient medium to obtain activating probio three times respectively probio lactobacillus acidophilus and Lactobacillus plantarum, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in the MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, transfer with 5% inoculum concentration at every turn, wherein all under 37 ℃ of condition of culture, cultivate 22h preceding twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, carry out microscopy then, survey the OD value, to activate for the third time behind the assorted bacterium of definite nothing and obtain activating probio
Get the lactobacillus acidophilus after the activation, Lactobacillus plantarum and Bifidobacterium are inserted the cysteine hydrochloride that adds 5g in the 10LMRS(MRS culture medium with 5% inoculum concentration) the fermentation tank culture 18h of culture medium, in incubation, stir once every 3h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-35 ℃ of pre-freeze 15h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 40% skimmed milk power, 12% trehalose, and 1% sodium hydrogen phosphate, 3% lactose and 44% phosphate buffer are formed;
5) by mass percentage with 70% goat milk powder, 16% pearl powder, grape pip, rose and burdock composite powder, 0.8% freeze-drying probiotics bacterial powder, it is 15 commentaries on classics/min that 6% sucrose, 6.2% glucose and 1% dolomol adopt rotating speed, power of motor is that the three-dimensional motion mixer of 0.5Kw mixes, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g;
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter: φ 14mm; Be 0.6g in the sheet; Hardness is at 4～6N.
Embodiment 5:
1) with grape pip, rose drying to constant weight, it is the rectangular of 5mm that fresh burdock is cut into width, adopts blast drier to be dried to constant weight at 70 ℃ then respectively;
2) pearl powder, grape pip, rose, burdock are met operative norm Q/12NK4558-2006 by the mass ratio mixing back employing of 1:2:2:2, power of motor is 1200W, it is Powdered that rotating speed is that the medicinal herb grinder of 2400r/min is ground into, and crosses 70 mesh sieves then and get pearl powder, grape pip, rose and burdock composite powder;
3) with raw material sheep breast through purify and standardization after decompression concentrate make solid content reach 50% raw material sheep breast, raw material sheep breast is crossed the sub goat milk powder that gets of 70 mesh sieves by spray-drying (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) back cool to room temperature;
4) first; the probiotic Lactobacillus acidophilus cultured at 37 ℃ conditions, using MRS liquid medium, activation times, to obtain activation probiotics; the Bifidobacterium in the MRS liquid medium, adding 0.05% medium volume cysteine hydrochloride, cultured at 37 ℃ conditions, using MRS liquid medium, activation times; each of 5% inoculum adapter; which the first two were cultured under the conditions at 37 ℃ 20h; s three times in the culture conditions of 37 ℃ for 18h; then microscopic examination, the measured OD value; determine the no bacteria after activation of the third time, to obtain activation of probiotics
Get Lactobacillus plantarum after the activation and Bifidobacterium and insert the cysteine hydrochloride that adds 5g in the 10L MRS(MRS culture medium with 5% inoculum concentration) the fermentation tank culture 18h of culture medium, in incubation, stir once every 4h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-20 ℃ of pre-freeze 24h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by 35% skimmed milk power, 15% trehalose, and 1% sodium hydrogen phosphate, 8% lactose and 41% phosphate buffer are formed;
5) by mass percentage with 63% goat milk powder, 20% pearl powder, grape pip, rose and burdock composite powder, 1% freeze-drying probiotics bacterial powder, it is 20 commentaries on classics/min that 7% sucrose, 8% glucose and 1% dolomol adopt rotating speed, power of motor is that the three-dimensional motion mixer of 0.5Kw mixes, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g;
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter: φ 10mm; Be 0.5g in the sheet; Hardness is at 4～6N.
Claims (9)
1. skin maintenance probio goat milk sheet, it is characterized in that: by the goat milk powder of mass percent 53%-78.9%, the pearl powder of 10%-30%, grape pip, rose and burdock composite powder, the freeze-drying probiotics bacterial powder of 0.1%-1%, the sucrose of 5%-7%, the glucose of 5%-8% and 1% dolomol are formed;
Described pearl powder, grape pip, rose and burdock composite powder are by pearl powder: grape pip: rose: burdock is that the mass ratio mixing of 1:2:2:2 obtains;
The probio of described freeze-drying probiotics bacterial powder is: the mixing of one or more arbitrary proportions of lactobacillus acidophilus, Bifidobacterium, Lactobacillus plantarum, Lactobacillus rhamnosus.
2. skin maintenance probio goat milk sheet according to claim 1, it is characterized in that: described freeze-drying probiotics bacterial powder is the mixture of probio and compound freeze drying protectant;
Wherein probio mixes with the ratio of the compound freeze drying protectant of 1mL with the every g probio of compound freeze drying protectant;
Probio is that viable count is 1.0-3.0 * 10
11Lactobacillus acidophilus bacterium powder, viable count be 1.0-3.0 * 10
11Bifidobacterium bacterium powder, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum bacterium powder, viable count be 1.0-3.0 * 10
10The Mixed Microbes of one or more arbitrary proportions of Lactobacillus rhamnosus bacterium powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed.
3. the preparation method of a skin maintenance probio goat milk sheet is characterized in that may further comprise the steps:
1) with grape pip, rose drying to constant weight, fresh burdock is cut into the rectangular constant weight that is dried to that width is 5mm;
2) Powdered with being ground into after pearl powder, grape pip, rose, the mass ratio mixing of burdock by 1:2:2:2, cross the 40-80 mesh sieve then and get pearl powder, grape pip, rose and burdock composite powder;
3) with the sheep breast through purify and standardization after, decompression concentrates and makes solid content reach 42～50% to obtain raw material sheep breast, get goat milk powder by crossing 40～80 mesh sieves behind spray-drying and the cool to room temperature;
4) get probio after the activation is inserted 10L MRS culture medium with 5% inoculum concentration fermentation tank culture 18h, in incubation, stir once every 3-4h, rotating speed 60 commentaries on classics/min, mixing time 2min, then, make probiotics bacterial mud with centrifuge with the centrifugal 12min of rotating speed of 8000 Zhuan ∕ min, in probiotics bacterial mud, press the compound freeze drying protectant that every g probio mud adds 1mL then, it is even to utilize the vortex mixed instrument to be mixed, at-40～-20 ℃ of pre-freeze 12～24h, move to freeze drier freeze drying 24h again and obtain freeze-drying probiotics bacterial powder;
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer are formed;
5) by mass percentage with the goat milk powder of 53%-78.9%, 10%-30% pearl powder, grape pip, rose and burdock composite powder, the freeze-drying probiotics bacterial powder of 0.1%-1%, 5%-7% sucrose, 5%-8% glucose and 1% dolomol mix, utilize one-shot formula tablet press machine then, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9The skin maintenance probio goat milk sheet of cfu/g.
4. the preparation method of skin maintenance probio goat milk sheet according to claim 3 is characterized in that: the drying of described step 1) adopts blast drier 70 ℃ of dryings.
5. the preparation method of skin maintenance probio goat milk sheet according to claim 3, it is characterized in that: described spray-drying EAT is 160～165 ℃, temperature of outgoing air is 75～65 ℃.
6. the preparation method of skin maintenance probio goat milk sheet according to claim 3, it is characterized in that: described probio is that viable count is 1.0-3.0 * 10
11Lactobacillus acidophilus bacterium powder, viable count be 1.0-3.0 * 10
11Bifidobacterium bacterium powder, viable count be 1.0-3.0 * 10
10Lactobacillus plantarum bacterium powder, viable count be 1.0-3.0 * 10
10The mixing of one or more arbitrary proportions of Lactobacillus rhamnosus bacterium powder.
7. the preparation method of skin maintenance probio goat milk sheet according to claim 3, it is characterized in that: the activation process of described probio is as follows:
Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus are adopted MRS fluid nutrient medium activation three times respectively under 37 ℃ of condition of culture, Bifidobacterium is added culture volume 0.05% in the MRS fluid nutrient medium cysteine hydrochloride adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, transfer with 5% inoculum concentration at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h preceding twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, carry out microscopy then, survey the OD value, will activate for the third time behind the assorted bacterium of definite nothing and obtain activating probio.
8. the preparation method of skin maintenance probio goat milk sheet according to claim 3 is characterized in that: it is 5～20 commentaries on classics/min that rotating speed is adopted in the mixing of described step 5), and power of motor is the three-dimensional motion mixer of 0.5Kw.
9. the preparation method of skin maintenance probio goat milk sheet according to claim 3 is characterized in that: the maximum compressing tablet pressure of described one-shot formula tablet press machine: 15KN; Compressing tablet diameter: φ 8～14mm; Be 0.4～0.6g in the sheet; Hardness is at 4～6N.
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| CN107455469A (en) * | 2017-09-30 | 2017-12-12 | 美可高特羊乳有限公司 | A kind of sheep breast composition with beauty functions and preparation method thereof |
| CN107668203A (en) * | 2017-10-17 | 2018-02-09 | 陕西科技大学 | A kind of snakeskin fruit yoghourt with beautifying face and moistering lotion and preparation method thereof |
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| CN102119726A (en) * | 2010-12-27 | 2011-07-13 | 陈慧婷 | Platycodon grandiflorum and burdock compound live bacteria containing yoghurt tablet for preventing pharyngitis and preparation method thereof |
| CN102440294A (en) * | 2010-10-15 | 2012-05-09 | 南通玺路贸易有限公司 | Water-soluble burdock residue dietary fiber milk powder candy |
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| CN102440294A (en) * | 2010-10-15 | 2012-05-09 | 南通玺路贸易有限公司 | Water-soluble burdock residue dietary fiber milk powder candy |
| CN102119726A (en) * | 2010-12-27 | 2011-07-13 | 陈慧婷 | Platycodon grandiflorum and burdock compound live bacteria containing yoghurt tablet for preventing pharyngitis and preparation method thereof |
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