CN105707233A - Preparation method of anti-oxidation Lactobacillus plantarum fermented goat milk - Google Patents

Preparation method of anti-oxidation Lactobacillus plantarum fermented goat milk Download PDF

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Publication number
CN105707233A
CN105707233A CN201610067228.1A CN201610067228A CN105707233A CN 105707233 A CN105707233 A CN 105707233A CN 201610067228 A CN201610067228 A CN 201610067228A CN 105707233 A CN105707233 A CN 105707233A
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caprae seu
seu ovis
preparation
lablab album
semen lablab
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陈合
惠翌昕
舒国伟
陈立
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Shaanxi University of Science and Technology
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Shaanxi University of Science and Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention discloses a preparation method of an anti-oxidation Lactobacillus plantarum fermented goat milk. The preparation method includes the following steps: seeding Lactobacillus plantarum strain suspension into reconstituted goat milk, adding white hyacinth bean polysaccharides into the reconstituted goat milk, and then performing anaerobic culture to obtain fermented goat milk; and adding sucrose into the fermented goat milk, and sealing the mixed fermented milk at 2 DEG C-5 DEG C to achieve after-ripening, thereby obtaining the anti-oxidation Lactobacillus plantarum fermented goat milk. The viable count of the anti-oxidation Lactobacillus plantarum fermented goat milk reaches 10^8 cfu/mL or above; the addition of the white hyacinth bean polysaccharides with antioxidant properties can promote the growth of probiotics and enhance the viscosity of goat milk, and also has effects in regulating the balance of intestinal microflora and the immunity of the human body, and the DPPH free radical scavenging rate of the anti-oxidation Lactobacillus plantarum fermented goat milk is 50%-80%; and long-term drinking the anti-oxidation Lactobacillus plantarum fermented goat milk prepared according to the above-mentioned method can prevent body oxidation caused a series of diseases including Alzheimer disease to some extent.

Description

A kind of preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis
Technical field
The present invention relates to the preparation method of a kind of Lac caprae seu ovis that ferments, particularly to a kind of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis Preparation method.
Background technology
Free radical is body one homergy product, has the strongest oxidability.But due to factors such as disease are old and feeble Free Radical Level can be made to raise, thus cause a series of disease.Therefore the research for antioxidant becomes focus.Its In natural anti-oxidation peptide become study hotspot owing to toxic and side effects is little, such as ascorbic acid, alpha-tocopherol, one A little non-oxidizability polysaccharide and the antioxidant in breast source.
The preparation method of anti-oxidation peptide mainly includes acid and alkali hydrolysis method, enzymatic isolation method and fermentation method at present, and acid and alkali hydrolysis method is dirty Dye is the most gradually eliminated relatively greatly, and enzymatic isolation method, due to efficient and gentle, is therefore most common method.But fermentation method is compared In enzymatic isolation method, maximum advantage is microorganism can be produced enzyme and the unification of enzymolysis process two step, has therefore saved cost, And the peptide prepared by fermentation method does not has bitterness, good in taste, the application being more suitable in food.
In Lac caprae seu ovis, lipochondrion is about 1/3rd of Lac Bovis seu Bubali, and more conducively human consumption absorbs, and wherein vitamin and The content of trace element apparently higher than Lac Bovis seu Bubali, and become be grouped into closer to human milk, easy allergy preferably, gastrointestinal function More weak and weakly drunk by people, it is described as " king in milk " in the world.Lactobacillus plantarum belongs to gram sun Property bacterium, amphimicrobian, is the one of homofermentative lactic bacteria, compared to having bigger viable bacteria for other lactic acid bacterias Number, and during the fermentation can high yield antioxidant, and itself also have a series of a series of health care make With, such as regulate immunity, maintain intestinal microbial population balance, reduction cholesterol formation, promotion absorption of nutrient ingredients etc. prebiotic Effect.Utilize Lactobacillus plantarum fermentation Lac caprae seu ovis, can be while obtaining the nutritive value of Lac caprae seu ovis itself, it is ensured that higher Number of live bacteria of probiotics and preferably non-oxidizability, and the health-care effect of probiotic bacteria itself.
Currently for using probiotics fermention method to prepare the less of non-oxidizability milk product, its Patent CN104186655A Provide and utilize blood pressure lowering and the preparation technology of non-oxidizability yoghurt product, to add the thickening thing such as pectin, modified starch The milk liquid mixture of matter be raw material, Lactobacillus plantarum and bifidobacterium strain be fermentation strain, successively inoculation ferment Prepare yogurt, but be not directed to using Lac caprae seu ovis as breast source, with this integration of edible and medicinal herbs of Semen Lablab Album polysaccharide and itself there is antioxygen The material of the property changed adds as thickening agent.Patent CN101575383A provides and utilizes decoction and alcohol sedimentation technique to prepare Semen Lablab Album The technique of polysaccharide, the method for not mentioned employing this milder highly effective and safe of enzyme process auxiliary law prepares Semen Lablab Album polysaccharide;Specially Profit CN103923835 provide utilize cellulase and pectase compound collecting Semen Lablab Album polysaccharide, be not directed to xylanase and The extracting method of cellulase compound use.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis, thus both prepare There is local flavor and the nutrition of classical acid Lac caprae seu ovis, there is again the functional sour codonopsis lanceolata compared with strong anti-oxidation.
To achieve these goals, the present invention uses techniques below:
1) bacteria suspension of lactobacillus plantarum strain is seeded to mass concentration answering as 10%-20% with the volume ratio of 3%-9% Argali Ruzhong, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 4-10mg/mL, then detest Oxygen is cultivated and is obtained the Lac caprae seu ovis that ferments;
2), after adding the sucrose of its quality 5%-10% in fermentation Lac caprae seu ovis, at 2 DEG C-5 DEG C, mixing cultured milk is carried out Seal and after-ripening, obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
Described Anaerobic culturel is Anaerobic culturel 6-15 hour in 35 DEG C of-45 DEG C of calorstats.
Described ripening time is 8h-12h.
Described Semen Lablab Album polysaccharide preparation technology is as follows:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 100-150 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add the cellulase of its quality 0.5%-1.5% in Semen Lablab Album solution, the wood of 0.5%-1.5% gathers Carbohydrase, regulation liquid-solid ratio be 40-60:1, pH be that 4-9 carries out enzymolysis, afterwards solution is heated to boiling and goes out Enzyme, filters enzymolysis solution subsequently, and collecting filtrate is Semen Lablab Album polysaccharide extraction liquid;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join dehydrated alcohol is carried out stand precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and dry at 45-60 DEG C being deposited in after washing with dehydrated alcohol Do to constant weight and pulverize, i.e. preparing Semen Lablab Album polysaccharide.
The activity of described cellulase is 8000U/g.
The activity of described xylanase is 50000U/g.
Described enzymatic hydrolysis condition is: 40 DEG C-70 DEG C carry out 2h-4h.
Described enzyme denaturing is for being heated to boiling and keeping 10-20min.
Described concentrated solution is 1:3-4 with the volume ratio of dehydrated alcohol, and the standing precipitate with ethanol time is 10-20h.
In the oxidation resistant plant lactobacillus ferment Lac caprae seu ovis that the present invention prepares, the viable count of Lactobacillus plantarum reaches 108More than cfu/mL.The Semen Lablab Album polysaccharide added not only itself has non-oxidizability, and can promote the life of probiotic bacteria Long, strengthen the viscosity of Lac caprae seu ovis, also there is scalable intestinal microbial population balance and the function of immunity of organisms.Oxidation resistant plant The DPPH free radical scavenging activity of lactobacillus ferment Lac caprae seu ovis is 50%-80%.The fermentation sour codonopsis lanceolata prepared according to the method described above, Through long-term drink can prevent to a certain extent due to body Oxidation cause such as a series of diseases such as senile dementias.
Detailed description of the invention
The lactobacillus plantarum strain of the present invention uses following methods to carry out activation processing: take 0.4g lyophilizing Lactobacillus plantarum Mycopowder is inoculated in 37 DEG C of constant temperature culture 24h in 10mL MRS culture medium, then activates for two generations with 5% inoculum concentration, obtains Three generations's bacteria suspension of Lactobacillus plantarum.
Embodiment 1:
Prepared by Semen Lablab Album polysaccharide:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 100 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add in Semen Lablab Album solution the activity of its quality 1% be the cellulase of 8000U/g, 1.5% The xylanase of 50000U/g, regulation liquid-solid ratio be 55:1, pH be at 50 DEG C of enzymolysis 3h after 6, afterwards by solution Being heated to boiling and keep 10min to carry out enzyme denaturing, being filtered by enzymolysis solution subsequently, collecting filtrate is Semen Lablab Album polysaccharide Extracting solution;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join the dehydrated alcohol of 3 times of volumes stand 10h precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited at 50 DEG C after washing with dehydrated alcohol dry to Constant weight is also pulverized, and i.e. prepares Semen Lablab Album polysaccharide.
Antioxidation bacillus acidophilus is fermented the preparation of Lac caprae seu ovis:
1) bacteria suspension of lactobacillus plantarum strain is seeded to, with the volume ratio of 5%, the recovery Lac caprae seu ovis that mass concentration is 14% In, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 6mg/mL, then in 41 DEG C of calorstats Anaerobic culturel obtains the Lac caprae seu ovis that ferments for 10 hours;
2) after adding the sucrose of its quality 5% in fermentation Lac caprae seu ovis, fermentation Lac caprae seu ovis is placed in 4 DEG C of lower seals after-ripening 10h, Obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
In products obtained therefrom, Lactobacillus plantarum viable count is 2.19 × 108Cfu/mL, DPPH free radical scavenging activity is 68.32%.
Embodiment 2:
Prepared by Semen Lablab Album polysaccharide:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 120 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add in Semen Lablab Album solution the activity of its quality 0.5% be the cellulase of 8000U/g, 1% The xylanase of 50000U/g, regulation liquid-solid ratio be 45:1, pH be at 60 DEG C of enzymolysis 2.5h after 5, afterwards by molten Liquid is heated to boiling and keeps 15min to carry out enzyme denaturing, is filtered by enzymolysis solution subsequently, and collecting filtrate is that Semen Lablab Album is many Sugar extracting solution;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join the dehydrated alcohol of 3 times of volumes stand 20h precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited at 60 DEG C after washing with dehydrated alcohol dry to Constant weight is also pulverized, and i.e. prepares Semen Lablab Album polysaccharide.
Antioxidation bacillus acidophilus is fermented the preparation of Lac caprae seu ovis:
1) bacteria suspension of lactobacillus plantarum strain is seeded to, with the volume ratio of 4%, the recovery Lac caprae seu ovis that mass concentration is 16% In, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 4mg/mL, then in 35 DEG C of calorstats Anaerobic culturel obtains the Lac caprae seu ovis that ferments for 15 hours;
2) after adding the sucrose of its quality 8% in fermentation Lac caprae seu ovis, fermentation Lac caprae seu ovis is placed in 3 DEG C of lower seals after-ripening 8h, Obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
In products obtained therefrom, Lactobacillus plantarum viable count is 4.06 × 108Cfu/mL, DPPH free radical scavenging activity is 71.40%.
Embodiment 3:
Prepared by Semen Lablab Album polysaccharide:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 150 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add in Semen Lablab Album solution the activity of its quality 1.5% be the cellulase of 8000U/g, 0.5% The xylanase of 50000U/g, regulation liquid-solid ratio be 60:1, pH be at 70 DEG C of enzymolysis 2h after 7, afterwards by molten Liquid is heated to boiling and keeps 20min to carry out enzyme denaturing, is filtered by enzymolysis solution subsequently, and collecting filtrate is that Semen Lablab Album is many Sugar extracting solution;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join the dehydrated alcohol of 4 times of volumes stand 15h precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited at 45 DEG C after washing with dehydrated alcohol dry to Constant weight is also pulverized, and i.e. prepares Semen Lablab Album polysaccharide.
Antioxidation bacillus acidophilus is fermented the preparation of Lac caprae seu ovis:
1) bacteria suspension of lactobacillus plantarum strain is seeded to, with the volume ratio of 3%, the recovery Lac caprae seu ovis that mass concentration is 12% In, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 8mg/mL, then in 38 DEG C of calorstats Anaerobic culturel obtains the Lac caprae seu ovis that ferments for 12 hours;
2) after adding the sucrose of its quality 6% in fermentation Lac caprae seu ovis, fermentation Lac caprae seu ovis is placed in 5 DEG C of lower seals after-ripening 11h, Obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
In products obtained therefrom, Lactobacillus plantarum viable count is 3.12 × 108Cfu/mL, DPPH free radical scavenging activity is 61.23%.
Embodiment 4:
Prepared by Semen Lablab Album polysaccharide:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 130 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add in Semen Lablab Album solution the activity of its quality 0.8% be the cellulase of 8000U/g, 1.2% The xylanase of 50000U/g, regulation liquid-solid ratio be 50:1, pH be at 40 DEG C of enzymolysis 4h after 4, afterwards by molten Liquid is heated to boiling and keeps 13min to carry out enzyme denaturing, is filtered by enzymolysis solution subsequently, and collecting filtrate is that Semen Lablab Album is many Sugar extracting solution;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join the dehydrated alcohol of 3.5 times of volumes stand 13h precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited at 55 DEG C after washing with dehydrated alcohol dry to Constant weight is also pulverized, and i.e. prepares Semen Lablab Album polysaccharide.
Antioxidation bacillus acidophilus is fermented the preparation of Lac caprae seu ovis:
1) bacteria suspension of lactobacillus plantarum strain is seeded to, with the volume ratio of 7%, the recovery Lac caprae seu ovis that mass concentration is 10% In, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 5mg/mL, then in 43 DEG C of calorstats Anaerobic culturel obtains the Lac caprae seu ovis that ferments for 6 hours;
2) after adding the sucrose of its quality 7% in fermentation Lac caprae seu ovis, fermentation Lac caprae seu ovis is placed in 2 DEG C of lower seals after-ripening 9h, Obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
In products obtained therefrom, Lactobacillus plantarum viable count is 4.15 × 108Cfu/mL, DPPH free radical scavenging activity is 67.04%.
Embodiment 5:
Prepared by Semen Lablab Album polysaccharide:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and be dissolved in 110 Part distilled water obtains Semen Lablab Album solution;
2) secondly, add in Semen Lablab Album solution the activity of its quality 1.2% be the cellulase of 8000U/g, 0.8% The xylanase of 50000U/g, regulation liquid-solid ratio be 40:1, pH be at 50 DEG C of enzymolysis 2.5h after 9, afterwards will Solution is heated to boiling and keeps 18min to carry out enzyme denaturing, is filtered by enzymolysis solution subsequently, and collection filtrate is Semen Lablab Album Polysaccharide extraction liquid;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentrated solution of White Hyacinth Bean equal in quality, so After join the dehydrated alcohol of 3 times of volumes stand 18h precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited at 50 DEG C after washing with dehydrated alcohol dry to Constant weight is also pulverized, and i.e. prepares Semen Lablab Album polysaccharide.
Antioxidation bacillus acidophilus is fermented the preparation of Lac caprae seu ovis:
1) bacteria suspension of lactobacillus plantarum strain is seeded to, with the volume ratio of 9%, the recovery Lac caprae seu ovis that mass concentration is 20% In, then be added thereto to Semen Lablab Album polysaccharide and make the concentration of Semen Lablab Album polysaccharide be 10mg/mL, then in 45 DEG C of calorstats Anaerobic culturel obtains the Lac caprae seu ovis that ferments for 12 hours;
2) after adding the sucrose of its quality 10% in fermentation Lac caprae seu ovis, fermentation Lac caprae seu ovis is placed in 4 DEG C of lower seals after-ripening 12h, Obtain oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
In products obtained therefrom, Lactobacillus plantarum viable count is 2.55 × 108Cfu/mL, DPPH free radical scavenging activity is 59.47%.

Claims (9)

1. the preparation method of an oxidation resistant plant lactobacillus ferment Lac caprae seu ovis, it is characterised in that:
1) with the volume ratio of 3%-9%, the bacteria suspension of lactobacillus plantarum strain being seeded to mass concentration is In the recovery Lac caprae seu ovis of 10%-20%, then it is added thereto to Semen Lablab Album polysaccharide and makes the concentration of Semen Lablab Album polysaccharide be 4-10mg/mL, then carries out Anaerobic culturel and obtains the Lac caprae seu ovis that ferments;
2) after adding the sucrose of its quality 5%-10% in fermentation Lac caprae seu ovis, to mixed fermentation at 2 DEG C-5 DEG C Breast carries out sealing and after-ripening, obtains oxidation resistant plant lactobacillus ferment Lac caprae seu ovis.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 1, it is special Levy and be: described Anaerobic culturel is Anaerobic culturel 6-15 hour in 35 DEG C of-45 DEG C of calorstats.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 1, it is special Levy and be: described ripening time is 8h-12h.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 1, it is special Levy and be: described Semen Lablab Album polysaccharide preparation technology is as follows:
1) first, take 5 parts of Semen Lablab Album size-reduced mistake 60 mesh sieves by mass fraction and obtain White Hyacinth Bean, and It is dissolved in 100-150 part distilled water and obtains Semen Lablab Album solution;
2) secondly, in Semen Lablab Album solution, the cellulase of its quality 0.5%-1.5%, 0.5%-1.5% are added Xylanase, regulation liquid-solid ratio be 40-60:1, pH be that 4-9 carries out enzymolysis, afterwards solution is heated Carrying out enzyme denaturing to boiling, filtered by enzymolysis solution subsequently, collecting filtrate is Semen Lablab Album polysaccharide extraction liquid;
3) rotated for Semen Lablab Album polysaccharide extraction liquid evaporimeter is concentrated into the concentration of White Hyacinth Bean equal in quality Liquid, is then added in dehydrated alcohol carry out standing precipitate with ethanol;
4) precipitate of standing is carried out sucking filtration, and will be deposited in after washing with dehydrated alcohol Dry to constant weight at 45-60 DEG C and pulverize, i.e. preparing Semen Lablab Album polysaccharide.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 4, it is special Levy and be: the activity of described cellulase is 8000U/g.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 4, it is special Levy and be: the activity of described xylanase is 50000U/g.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 4, it is special Levy and be: described enzymatic hydrolysis condition is: 40 DEG C-70 DEG C carry out 2h-4h.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 4, it is special Levy and be: described enzyme denaturing is for being heated to boiling and keeping 10-20min.
The preparation method of oxidation resistant plant lactobacillus ferment Lac caprae seu ovis the most according to claim 4, it is special Levying and be: described concentrated solution is 1:3-4 with the volume ratio of dehydrated alcohol, the standing precipitate with ethanol time is 10-20h.
CN201610067228.1A 2016-02-01 2016-02-01 Preparation method of anti-oxidation Lactobacillus plantarum fermented goat milk Pending CN105707233A (en)

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CN106417599A (en) * 2016-09-14 2017-02-22 光明乳业股份有限公司 Fermented product added with lactobacillus plantarum proliferation agent and preparation method thereof
CN107197944A (en) * 2017-07-13 2017-09-26 云南农业大学 One kind fermentation sheep breast and its zymotechnique
CN107771940A (en) * 2017-10-17 2018-03-09 陕西科技大学 A kind of preparation method of oxidation resistant Lactobacillus casei fermentation sheep breast
CN108935709A (en) * 2018-07-16 2018-12-07 陕西科技大学 A kind of probiotics goat milk piece and preparation method thereof
CN110122567A (en) * 2019-04-26 2019-08-16 石家庄君乐宝乳业有限公司 Composite fermentation cream with anti-oxidation function and preparation method thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106417599A (en) * 2016-09-14 2017-02-22 光明乳业股份有限公司 Fermented product added with lactobacillus plantarum proliferation agent and preparation method thereof
CN107197944A (en) * 2017-07-13 2017-09-26 云南农业大学 One kind fermentation sheep breast and its zymotechnique
CN107771940A (en) * 2017-10-17 2018-03-09 陕西科技大学 A kind of preparation method of oxidation resistant Lactobacillus casei fermentation sheep breast
CN108935709A (en) * 2018-07-16 2018-12-07 陕西科技大学 A kind of probiotics goat milk piece and preparation method thereof
CN110122567A (en) * 2019-04-26 2019-08-16 石家庄君乐宝乳业有限公司 Composite fermentation cream with anti-oxidation function and preparation method thereof

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