CN1899314A - Process for preparing lactic acid bacteria oral liquor - Google Patents
Process for preparing lactic acid bacteria oral liquor Download PDFInfo
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- CN1899314A CN1899314A CNA2006100452911A CN200610045291A CN1899314A CN 1899314 A CN1899314 A CN 1899314A CN A2006100452911 A CNA2006100452911 A CN A2006100452911A CN 200610045291 A CN200610045291 A CN 200610045291A CN 1899314 A CN1899314 A CN 1899314A
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Abstract
The present invention relates to health food, and is especially preparation process of one kind of lactic acid bacteria oral liquid. The lactic acid bacteria oral liquid is prepared through mixing brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride in certain proportion, dissolving in water and heating to sterilize; inoculating one or several of Lactobacillus casei subsp. Casei, Lactobacillus plantarum, Lactobacillus acidophilus and Bacillus coagulans; culturing at 37deg.c for 48 hr; harvesting after the pH value is lowered to about 3.6; and bacteria-free packing. The lactic acid bacteria oral liquid is brown and sour and has live lactic acid bacteria content up to 1 billion/ml. It is used in improving digestive tract function and treating gastrointestinal tract ailments.
Description
(1) technical field
The present invention relates to a kind of preparation method of lactobacillus oral liquid, belong to field of health care food.
(2) background technology
Along with deepening continuously of related science research, lactobacillus is disclosed for the benefit of health more and more, as promoting body to the microecological balance that absorbs, keeps intestinal microbial population of nutritional labeling, enhancing human body immunity function etc.
Its major function is as follows:
1, regulates intestinal microbial population
Lactobacillus has suppressed breeding and the field planting of pathogen by producing a large amount of organic acid, hydrogen peroxide, enzyme, bacteriocin etc., has promoted enterokinesia, keeps intestinal microecology and normal physiological function.Lactobacillus is the profitable strain of settling down in intestinal, and the obligate anaerobe that with the lactobacillus is representative is the main strength that constitutes the field planting drag.Lactobacillus produces acid by metabolism and reduces the effect that the enteral pH value has also played the harmful bacterium breeding of inhibition.
2, enhancing immunity
Lactobacillus is the phagocytosis of activating macrophage obviously, simultaneously can also activate bone-marrow-derived lymphocyte and NK cell, increase the generation of IgA in the intestinal, increase production of cytokines amounts such as IL-1, IL-2, IL-5, IL-6, TNF-α, stimulate human body to produce nonspecific immunity; Because it can field planting in intestinal, be equivalent to natural active immunity on the other hand.They can also stimulate peritoneal macrophages in addition, produce interferon, promote cell division, produce antibody and cellular immunization etc., thus the non-specific and specific immune response of energy enhancing body, the resistance against diseases of raising body.When foreign body is invaded body,, then there is enhancing body foreign body to be produced the effect of antibody by the activated immunocyte of lactobacillus.
Therefore, lactobacillus beverage has the health care of good enhancing immunity, adjusting intestinal microbial population.
Producing lactobacillus beverage at present mainly is to produce yoghourt, with raw material be milk, strain is Lactobacillus plantarum, bacillus acidophilus, Lactobacillus bulgaricus, lactobacillus casei, streptococcus faecalis, and also not utilizing brown sugar, glucose so far is that raw material prepares lactobacillus oral liquid.
(3) summary of the invention
At the deficiencies in the prior art, the invention provides a kind of preparation method of new lactobacillus oral liquid.
The preparation method of lactobacillus oral liquid of the present invention, put it briefly and be, the present invention utilizes brown sugar, raw materials such as glucose, strain is selected from lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei (bacterium numbering: CGMCC1.2435), Lactobacillus plantarum Lactobacillus plantarum (bacterium numbering: CGMCC1.103), bacillus acidophilus Lactobacillus acidophilus (bacterium numbering: CGMCC 1.1854), (bacterium numbering: CGMCC1.2009) one of four kinds of probiotic bacterias or combination prepare lactobacillus oral liquid by microbial fermentation to Bacillus coagulans Bacilluscoagulans.Above-mentioned 4 kinds of probiotics strains are all available from Chinese common micro-organisms culture presevation administrative center.This product activity composition is one of above-mentioned 4 kinds of probiotic bacterias or combination.
The preparation method of lactobacillus oral liquid of the present invention, step is as follows:
(1) by weight table sugar 1-10 part, edible glucose 1-10 part, peptone 0.1-2 part, magnesium sulfate 0.01-0.2 part, manganese sulfate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-1 part, sodium chloride 0.1-2 part are mixed, add water 75-98 part dissolving of purification, heat treatment method is sterilized, as culture medium;
(2) on above-mentioned culture medium, insert one of following strain or its combination, inoculum concentration is 0.5-2 part, weight portion: lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei (bacterium numbering: CGMCC1.2435), Lactobacillus plantarum Lactobacillus plantarum (bacterium numbering: CGMCC1.103), bacillus acidophilus Lactobacillus acidophilus (bacterium numbering: CGMCC1.1854), Bacillus coagulans Bacillus coagulans (bacterium numbering: CGMCC1.2009);
(3) the postvaccinal mixed liquor of step (2) was cultivated 48 hours at 37 ℃, got final product when pH value is reduced to 3.4-4.0;
(4) product of step (3) is aseptic subpackaged, make lactobacillus oral liquid.
Preferably, the described heat treatment of step (1) is to carry out under 110-128 ℃ temperature, and sterilization time is 25-180 minute.
Preferably, the described table sugar of step (1) is a brown sugar.
The lactobacillus oral liquid of the inventive method preparation is a brown liquid, and sour in the mouth is about viable bacteria content 1,000,000,000/mL.Be specially adapted to improve dyspepsia, malabsorption, have no appetite, gastrointestinal problems such as diarrhoea, constipation, intestinal tympanites.
It is that raw material prepares lactobacillus oral liquid that the present invention selects brown sugar, glucose first for use.When used lactobacillus casei cheese subspecies are fermented separately, can in 48 hours, pH value be dropped to 3.40, when Lactobacillus plantarum ferments separately, can in 48 hours, pH value be dropped to 3.6, when the bacillus acidophilus is fermented separately, can in 48 hours, pH value be dropped to 3.9, when Bacillus coagulans is fermented separately, can in 48 hours, pH value be dropped to 3.5.Four strain bacterium Mixed culture can drop to 3.6 with pH value in 48 hours.The inventive method prepares lactobacillus oral liquid, produces acid soon, probiotic bacteria content ideal.
(4) description of drawings
Fig. 1 is a lactobacillus casei cheese subspecies single strain fermentation and acid curve, Fig. 2 is a Lactobacillus plantarum single strain fermentation and acid curve, Fig. 3 is bacillus acidophilus's single strain fermentation and acid curve, Fig. 4 is a Bacillus coagulans single strain fermentation and acid curve, and the love song line is produced in Fig. 5 lactobacillus casei cheese subspecies, Lactobacillus plantarum, bacillus acidophilus and four kinds of bacterial strain mixed culture fermentations of Bacillus coagulans.Abscissa is fermentation time (unit: hour) among the figure, and vertical coordinate is a pH value.
(5) specific embodiment
The present invention will be further described below in conjunction with embodiment, but be not limited thereto.The consumption unit of each component is weight portion among the embodiment 1-5.
Embodiment 1: lactobacillus casei cheese subspecies single strain fermenting process of preparing lactobacillus oral liquid
Present embodiment selects for use lactobacillus casei cheese already to plant independent fermentation, can in 48 hours pH value be dropped to about 3.6.Preparation method: with 2 parts in brown sugar, 5 parts of glucoses, 0.2 part of peptone, 0.01 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.01 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.2 part, add the tap water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of lactobacillus casei cheese subspecies seed liquor then, cultivated 48 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 1.The gained lactobacillus oral liquid is a brown liquid, sour in the mouth, viable bacteria content 1,000,000,000/mL.
Embodiment 2: Lactobacillus plantarum single strain fermenting process of preparing lactobacillus oral liquid
Present embodiment selects for use Lactobacillus plantarum to ferment separately, can in 40 hours pH value be dropped to about 3.7.Preparation method: with 2 parts in brown sugar, 5 parts of glucoses, 0.2 part of peptone, 0.01 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.01 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.2 part, add the tap water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of Lactobacillus plantarum seed liquor then, cultivated 48 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 2.The gained lactobacillus oral liquid is a brown liquid, sour in the mouth, viable bacteria content 1,000,000,000/mL.
Embodiment 3: bacillus acidophilus's single strain fermenting process of preparing lactobacillus oral liquid
Present embodiment selects for use the bacillus acidophilus to ferment separately, can in 30 hours pH value be dropped to about 3.9.Preparation method: with 2 parts in brown sugar, 3 parts of glucoses, 0.1 part of peptone, 0.02 part in magnesium sulfate, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the tap water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of bacillus acidophilus's seed liquor then, cultivated 30 hours for 37 ℃, pH reduces to 3.9 o'clock results, bottling.Produce the love song line and see Fig. 3.The gained lactobacillus oral liquid is a brown liquid, sour in the mouth, viable bacteria content 1,000,000,000/mL.
Embodiment 4: Bacillus coagulans single strain fermenting process of preparing lactobacillus oral liquid
Present embodiment selects for use Bacillus coagulans to ferment separately, can in 37 hours pH value be dropped to about 3.6.Preparation method: with 3 parts in brown sugar, 4 parts of glucoses, 0.2 part of peptone, 0.02 part in magnesium sulfate, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the tap water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of Bacillus coagulans seed liquor then, cultivated 37 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 4.The gained lactobacillus oral liquid is a brown liquid, sour in the mouth, viable bacteria content 1,000,000,000/mL.
Embodiment 5: lactobacillus casei cheese subspecies, Lactobacillus plantarum, bacillus acidophilus, four kinds of probiotic bacterias of Bacillus coagulans were by 1: 1: 1: 1 volume ratio combined inoculation fermenting process of preparing lactobacillus oral liquid.
Present embodiment selects for use lactobacillus casei cheese subspecies, Lactobacillus plantarum, bacillus acidophilus, four kinds of probiotic bacterias of Bacillus coagulans by 1: 1: 1: 1 volume ratio combined inoculation fermentation can drop to pH value about 3.5 in 28 hours.Preparation method: with 5 parts in brown sugar, 3 parts of glucoses, 0.2 part of peptone, 0.02 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the tap water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert lactobacillus casei cheese subspecies, Lactobacillus plantarum, bacillus acidophilus, four kinds of probiotic bacterias of Bacillus coagulans then by 1: 1: 1: totally 1 part of 1 volume ratio seed mixture liquid, cultivated 28 hours for 37 ℃, pH reduces to 3.5 o'clock results, bottling.Produce the love song line and see Fig. 5.The gained lactobacillus oral liquid is a brown liquid, sour in the mouth, viable bacteria content 1,000,000,000/mL.
Embodiment 6:
For understanding lactobacillus oral liquid, observed the 55 routine infant clinical therapeutic efficacy of suffering from diarrhoea autumn and winter to infant diarrheal autumn and winter curative effect.This group data is the diarrhoea sick child that Shandong hospital is in hospital, June at age～2 years old; Acute onset, the course of disease are in 3 days, and clinical symptoms is with heating, diarrhoea, abdominal distention or companion's mild dehydration, and diarrhoea number of times every day is more than 4 times; The stool character is egg soup sample or rare water sample, and routine examination has Oil globule or a little leukocyte, no erythrocyte, and pyocyte, parasites etc. accompany or do not accompany upper respiratory tract infection.
This group data is divided into 2 groups at random, and 30 examples are organized in treatment, male 20 examples, women 10 examples, age＜1 year old 16 examples; Matched group 25 examples, male 15 examples, women 10 examples, age＜1 year old 12 examples.Two groups of case stochastic sampling, sex, age, the course of disease, diarrhoea number of times, clinical symptoms are learned the processing there was no significant difference by statistics.The lactobacillus oral liquid to embodiment 5 preparation is organized in treatment, dosage:＜1 years old every day 1 time, each 1 bottle (10mL); 2 bottles of 1～2 year old every days, divide 2 times oral.Serve on 3d, without antibiotic.Matched group gives gentamycin and bismuth subcarbonate is oral, and gentamycin divides oral 2% gentamycin solution 3～4 times by 10mg/kg calculating every day; Bismuth subcarbonate＜1 years old 0.15g/ time, 3 times/day; 1～2 year old 0.2g/ time, 3 times/day; Be 3 days the course of treatment.Allly take medicine that sb.'s illness took a favorable turn but do not heal, then took medicine again 2～3 days.Two groups of whiles add according to the state of an illness uses oresol, and the hyperpyrexia person gives Physical temperature-lowering or drug cooling.
Efficacy assessment standard
Produce effects: the 24～48h that takes medicine, times of defecation be reduced to≤and 2 times/day or return to normal number of times, the stool character recovers normal, the clinical symptoms complete obiteration.Effectively: the 48～72h that takes medicine, times of defecation just are reduced to 2 times/day, and the stool character takes a turn for the better, and clinical symptoms disappears substantially.Invalid: the back 72h diarrhoea number of times still＞2 time/day of taking medicine, or character do not have and is clearly better, even the state of an illness increases the weight of and the person of changing dressings.
Clinical effectiveness sees Table 1.
Table 1 liang group efficacy result relatively
| n | Produce effects example number (%) | Effective routine number (%) | Invalid routine number (%) | Effective percentage (%) | |
| Treatment group matched | 30 25 | 26(86.7) 9(36) | 4(13.3) 5(20) | Do not have 11 (44) | 100 56 |
Check two groups of comparing differences significantly (P＜0.01) through x2
The result shows that treatment group effective percentage reaches 100%, and it is remarkable as seen to take the lactobacillus oral liquid effect.
Embodiment 7:
The acute toxicity test of lactobacillus oral liquid per os: this test is undertaken by HSPH of Shandong Medical University toxicologic study chamber.With feed 60 of Kunming mouses of lactobacillus oral liquid stock solution of the present invention, body weight 18-22g, male and female half and half.Mice is divided into 6 groups at random, 10 every group, male and female half and half, behind the fasting 12h, per os is irritated stomach, and dosage is respectively 40mL/kg, 32mL/kg, 25.6mL/kg, 20.5mL/kg, 16.4mL/kg, 13.1mL/kg (r=0.8).Observed 14 days continuously after the mice administration, record mice poisoning symptom and death toll according to each group mouse death rate, are calculated LD with the Bliss method
50With 95% fiducial limit.The results are shown in Table 2.
Table 2 lactobacillus oral liquid acute toxicity test in mice result
| Dosage (mL/kg) | Mus number (only) | Dead Mus number (only) | Mortality rate (5) |
| 40 32 25.6 20.5 16.4 13.1 | 10 10 10 10 10 10 | 0 0 0 0 0 0 | 0 0 0 0 0 0 |
The chmice acute toxic reaction: per os is to the no abnormal phenomenon of mice behind the animal subject.
The result shows that per os of mice gives lactobacillus oral liquid half lethal dose LD
50Greater than 40mL/kg.
Claims (3)
1, the preparation method of lactobacillus oral liquid, step is as follows:
(1) by weight table sugar 1-10 part, edible glucose 1-10 part, peptone 0.1-2 part, magnesium sulfate 0.01-0.2 part, manganese sulfate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-1 part, sodium chloride 0.1-2 part are mixed, add water 71.6-97.8 part dissolving of purification, heat treatment method is sterilized, as culture medium;
(2) insert one of following strain or its combination on above-mentioned culture medium, inoculum concentration is 0.5-2 part, weight portion:
Lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei, bacterium numbering: CGMCC1.2435,
Lactobacillus plantarum Lactobacillus plantarum, bacterium numbering: CGMCC1.103,
Bacillus acidophilus Lactobacillus acidophilus, bacterium numbering: CGMCC1.1854,
Bacillus coagulans Bacillus coagulans, bacterium numbering: CGMCC1.2009;
(3) the postvaccinal mixed liquor of step (2) was cultivated 48 hours at 37 ℃, got final product when pH value is reduced to 3.4-4.0;
(4) product of step (3) is aseptic subpackaged, make lactobacillus oral liquid.
2, the preparation method of lactobacillus oral liquid as claimed in claim 1 is characterized in that the described heat treatment of step (1) is to carry out under 110-128 ℃ temperature, sterilization time is 25-180 minute.
3, the preparation method of lactobacillus oral liquid as claimed in claim 1 is characterized in that the described sugar of step (1) is brown sugar.
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| CN101977615A (en) * | 2008-03-19 | 2011-02-16 | 森下仁丹株式会社 | Inhibitor for blood phosphorus level elevation |
| CN101700061B (en) * | 2009-10-28 | 2012-06-27 | 山东康智多生物科技有限公司 | High-activity fermented milk beverage and preparation method thereof |
| CN103622026A (en) * | 2013-12-18 | 2014-03-12 | 青岛根源生物技术集团有限公司 | Nutritional oral solution containing probiotics and preparation method thereof |
| CN105831534A (en) * | 2016-03-28 | 2016-08-10 | 济南康多宝生物技术有限公司 | Compound lactic acid bacteria beverage and a preparation method thereof |
| CN106858596A (en) * | 2017-02-10 | 2017-06-20 | 天津金匮堂生物科技有限公司 | A kind of probiotic acid jujube oral liquid and preparation method thereof |
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Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1105544A (en) * | 1994-09-09 | 1995-07-26 | 扬州大学农学院 | Method for preparing active lactic acid bacteria oral liquor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101977615A (en) * | 2008-03-19 | 2011-02-16 | 森下仁丹株式会社 | Inhibitor for blood phosphorus level elevation |
| CN101977615B (en) * | 2008-03-19 | 2012-11-21 | 森下仁丹株式会社 | Inhibitor for blood phosphorus level elevation |
| US9056122B2 (en) | 2008-03-19 | 2015-06-16 | Morishita Jintan Co., Ltd. | Method for inhibition of blood phosphorus level elevation |
| CN101700061B (en) * | 2009-10-28 | 2012-06-27 | 山东康智多生物科技有限公司 | High-activity fermented milk beverage and preparation method thereof |
| CN103622026A (en) * | 2013-12-18 | 2014-03-12 | 青岛根源生物技术集团有限公司 | Nutritional oral solution containing probiotics and preparation method thereof |
| CN105831534A (en) * | 2016-03-28 | 2016-08-10 | 济南康多宝生物技术有限公司 | Compound lactic acid bacteria beverage and a preparation method thereof |
| CN106858596A (en) * | 2017-02-10 | 2017-06-20 | 天津金匮堂生物科技有限公司 | A kind of probiotic acid jujube oral liquid and preparation method thereof |
| CN110464741A (en) * | 2019-02-25 | 2019-11-19 | 山东中微众康生物科技有限公司 | A kind of lactic acid bacteria oral liquor and preparation method thereof improving human body intestinal canal health |
| CN110463892A (en) * | 2019-02-25 | 2019-11-19 | 山东中微众康生物科技有限公司 | A kind of lactic acid bacteria solid beverage and preparation method thereof improving human body intestinal canal health |
| CN109700033A (en) * | 2019-03-04 | 2019-05-03 | 山东环亿生物科技有限公司 | A kind of adjusting enteron aisle, probiotic composition of prevent constipation and preparation method thereof |
| CN109700033B (en) * | 2019-03-04 | 2021-06-04 | 山东环亿生物科技有限公司 | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof |
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