CN100450375C - Composite microorganism type forage additives and its prodn. method - Google Patents
Composite microorganism type forage additives and its prodn. method Download PDFInfo
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- CN100450375C CN100450375C CNB2006100468869A CN200610046886A CN100450375C CN 100450375 C CN100450375 C CN 100450375C CN B2006100468869 A CNB2006100468869 A CN B2006100468869A CN 200610046886 A CN200610046886 A CN 200610046886A CN 100450375 C CN100450375 C CN 100450375C
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- lactic acid
- acid bacteria
- bacillus subtilis
- xylo
- oligosaccharide
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- 238000000034 method Methods 0.000 title description 8
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Landscapes
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a compound micro-ecological additive for feed, which is prepared by mixing 0.1 to 0.4 part of oligomerization xylose, 0.1 to 0.4 part of oligomerization chitosan, 0.01 to 0.2 part of freeze drying powder of lactic acid bacteria, 0.05 to 0.5 part of nutrient material of saccharomycete and 0.02 to 0.4 part of bacillus subtilis by weight. The compound micro-ecological additive for feed has the functions of preventing and treating diarrhea, promoting growth, inhibiting and reducing putrefying bacteria and pathogenic bacteria and reducing the exhaust of harmful gases in feces. The product can reduce the pollution of animal husbandry to an environment, prevent the medicine residue of antibiotics, etc. in livestock products and produce livestock products without environmental disruption.
Description
Technical field
The invention belongs to technical field of animal, particularly microorganism formulation is as the technology of feed addictive and method for making thereof.
Background technology
Be to have found the animal breeding to begin to pay attention to the research and development and the use of additive for microbe feedstuff after antibiotic all drawbacks in the world from the sixties.The U.S. brings into use microorganism fodder from the seventies, the microorganism that allowance is at present directly fed has kind more than 40, and year use amount is about more than 8000 tons.Only French probiotic kind of selling on the market in 1991 just is not less than 50 in Europe.Countries such as day, moral, English developed novel antibiotic substitute products in succession and have dropped into and produce in batches before more than 10 years, microorganism formulation is developed faster.Some countries of the U.S. and West Europe forbid or limit the use of feeding antibiotic in succession, have promoted the development of microorganism formulation industry greatly.In addition, abroad aspect using microbe feed addictive production fluid biological feedstuff, industrialized development is also very fast.Denmark, Holland, Britain, the U.S. progressively promote this technology.From technical standpoint, adopt liquid fermentation abroad, efficiently solve unstable product quality, living bacteria count deficiency, problems such as contamination of products; Adopt low-temperature vacuum drying and micro-capsule coating technique, changed the situation that the product appearance quality is coarse, the shelf time is short.From external exploitation and operating position, external product is wide in variety.The probiotic of single bacterial strain is less, and more pays attention to the research of composite bacteria preparation.Because single bacteria preparation is easy to by imitated, prior reason is that the action effect of composite bacteria preparation is better, more realistic ecological environment on the one hand for this.Composite bacteria preparation generally all has synergy, synergy with cured sample bud pole bacterium and lactic acid bacteria is an example, cured sample bud pole bacterium is a height oxygen consumption bacterium, anaerobic environment can suppress the pathogenic bacteria growth in the body that it causes, but help the growth of the lactic acid bacteria of anaerobism, the growth of lactic acid bacteria has increased the acidity of environment again, has more strengthened the inhibition to the pathogenic bacteria growth.Though the product price is higher, its addition is low.
In China, the research of probiotics starts from the seventies, by the first proposition of being beheaded of what bright Puritanism of Sichuan Agricultural University, the later stage eighties just begins to pay attention to the research and development of additive for microbe feedstuff, many single or compound preparations based on Bacillus acidi lactici, streptococcus fecalis, bacillus and saccharomycete etc.China set up Chinese microecology meeting in 1992, and feeding micro-ecological preparation and application technical research thereof are listed in country " eight or five " tackling of key scientific and technical problems problem, had just promoted the application study work of microorganism formulation.The additive for microbe feedstuff of domestic present production still exists bigger gap with external product in many aspects, mainly show: domestic additive for microbe feedstuff adopts solid fermentation more, make many unstable product quality, the living bacteria count deficiency, contamination of products (sometimes even in product, detect a large amount of moulds); The direct oven drying method of domestic employing makes that the product appearance quality is coarse, the shelf time is short; The home products good and the bad differs, too exaggerative its effect of the description of product, and actual effect is not obvious; In addition, the addition of home products is higher.
Summary of the invention
Xylo-oligosaccharide and chitosan oligomer are referred to as prebiotics (Prebiotics).
Xylo-oligosaccharide (Xylo-oligosaccharide) is the Oilgosaccharkdes that increment Bifidobacterium effect is best in the functional oligose of generally acknowledging both at home and abroad at present, consumption is minimum, selectivity is the strongest.The general name of the xylo-oligosaccharide functional polymerization sugar that to be 2-7 wood sugar be formed by connecting with β-1,4 glycosidic bond.Its sugariness is equivalent to 30% of sucrose; xylo-oligosaccharide has very high heat endurance (80～150 ℃ of heating still kept 100% integrality in 20 minutes); absolute acid stability (placed 20 minutes in 100 ℃ by pH2.3～8.0 time; its composition is almost constant); storage-stable (stored two months in 37 ℃ by pH2.5～8.0 time; its composition is almost constant); xylo-oligosaccharide has high selectivity and promotes the Bifidobacterium increment; do not decomposed by animal digestive ferment system; suppressing harmful bacterium and tunning thereof generates; the protection liver; strengthen immunity of organisms; promote calcium to absorb; without any side effects, good compatibility ability is arranged.Xylo-oligosaccharide is a kind of water-soluble dietary fiber.
Chitosan oligomer (Chitosan) is the high molecular polymer that chitin (Chitin) is sloughed the water-soluble nitrogenous polysaccharide that the acetyl group in (1-4)-2-acetamide-2-deoxidation-D-dextran molecule forms.It has nontoxic, tasteless, sun-proof, heat-resisting, corrosion-resistant, and is not afraid of and damages by worms and the characteristic of the etch of alkali.The biologically active of shitosan comprises: reduce cholesterol, and blood fat, immunity strengthens, and is antimicrobial, antitumor, effects such as anticoagulation and promotion tissue repair.There is the test of meat chick to show: to add shitosan and can reduce the absorption of fat and reduce abdominal fat weight, but do not influence growth, therefore, in the animal daily ration, add shitosan and can control the fat deposition amount, and do not reduce efficiency of feed utilization.Fat content also shows in contrast serum and the muscle, shitosan is except having the effect that reduces cholesterol, some unrighted acid also there is certain reduction effect, and in daily ration, add certain amount of chitosan, growth performance to animal does not have influence, and can produce the low cholesterol livestock products.
Freeze-dried lactic acid bacteria (Lactobacillus) powder, saccharomycete (Yeast) culture, bacillus subtilis (BacillusSubtilis) general designation probio (Probiotics) preparation.
Probiotics preparation has characteristics such as have no side effect, have no drug resistance, noresidue, low cost, growth promotion effect are remarkable.Probiotics preparation can replenish in the enteron aisle the normal microorganism that reduces or lack on quantity or kind, adjust or keep microecological balance in the enteron aisle, promotes beneficial bacterium propagation such as lactic acid bacteria, suppresses Escherichia coli etc. and causes pathogen growth.Probiotics preparation can strengthen body's immunity, promote the absorption of nutriment to transform, these beneficial microbes can produce various digestive ferments and active material in vivo, thereby improve the utilization of nutriment, the booster immunization function is used probiotics preparation and can be reached and prevent and cure diseases, improve food conversion ratio and the purpose of livestock and poultry production performance.
The present invention provides a kind of composite microorganism type forage additives in order to overcome the technological deficiency of prebiotics (Prebiotics) preparations such as independent use compound sugar and probio (Probiotics) preparation---Synbiotics (Synbiotics) and processing method.
Technical scheme of the present invention is: get xylo-oligosaccharide 0.10～0.40 weight portion, chitosan oligomer 0.10～0.60 weight portion, freeze-dried lactic acid bacteria powder 0.01～0.20 weight portion, saccharomycete culture 0.05～0.50 weight portion, bacillus subtilis 0.02～0.50 weight portion and be added in the mixer, fully be mixed into pulvis.Find in the practice that following material rate is preferably come out: xylo-oligosaccharide 0.10～0.40 weight portion, chitosan oligomer 0.20～0.40 weight portion, freeze-dried lactic acid bacteria powder 0.01～0.05 weight portion, saccharomycete culture 0.05～0.15 weight portion, bacillus subtilis 0.02～0.50 weight portion are mixed into pulvis.Representative formula is: xylo-oligosaccharide 0.2 weight portion, chitosan oligomer 0.2 weight portion, freeze-dried lactic acid bacteria powder 0.2 weight portion, saccharomycete culture 0.1 weight portion, bacillus subtilis 0.2 weight portion are mixed into pulvis.
Processing method for this composite microorganism type forage additives is, take xylo-oligosaccharide, chitosan oligomer food grade materials that enzyme solution is produced respectively, lactic acid bacteria is in cryogenic temperature-30～65 ℃, make under vacuum 20～60Pa bacterium powder, yeast culture with 30～70 ℃ of oven dry, bacillus subtilis with 50～90 ℃ of oven dry; Preferably lactic acid bacteria is in cryogenic temperature-20～65 ℃, making bacterium powder, yeast culture under vacuum 15～45Pa mixes with the mixture homogeneity coefficient of variation less than 5% the efficient mixer of twin shaft paddle formula with 40～60 ℃ of oven dry products with 35～55 ℃ of oven dry, bacillus subtilis, package paper is moulded and the clad aluminum foil bag, storage temperature is less than 35 ℃, and storage condition humidity is less than 60%.
The characteristics of product composite microorganism type forage additives compared with prior art of the present invention are:
One, consumption is few, and mixed feed per ton adds 100～800 grams, saves the feed formula space, reduces feed cost.Common active bacteria formulation additive capacity is big, and interpolation 2000～3000 per ton restrains, and converts to reach 8000～12000 grams in the concentrate feed, and it is big to take the prescription space, and cost is higher, uses this product can overcome the problems referred to above.
Two, effect is remarkable, has tangible growth promotion, control to suffer from diarrhoea, obviously reduce the mew smell.Beneficial bacterium in the product can stimulate the foundation early of animal immune system, transfers and improves the general nonspecific immunity function of animal body, thereby improved whole resistance against diseases.The activity and the huge cytophilic activity that studies have shown that interferon in the animal body of having taken probiotics (containing lactic acid bacteria) all increase.Beneficial bacterium in the product can produce enzyme and nutriment, beneficial bacterium can produce various digestive ferments in vivo, especially some bacillus has very strong protease, lipase, amylase activity, and the ANFs in the degradable feed improves the conversion ratio of feed greatly.In addition, the organic acid that beneficial bacterium produces can improve the activity of acid protease, strengthens the digestion power of animal to protein, and is very useful to newborn livestock and poultry.Beneficial bacterium can produce multiple nutrients material such as vitamin (especially B family vitamin), amino acid, the unknown growth factor etc. that promotes in the growth and breeding process in enteron aisle, supplement the nutrients, and promotes growth of animal.Bacillus subtilis can produce amino oxidase and decompose the enzyme of sulfide in enteron aisle, thereby reduces harmful gas concentrations such as ammonia, indoles in blood and the ight soil.
Three, nontoxic, also can reduce poisonous and hazardous Application of Additives, reduce the antibiotic residue in the livestock products to greatest extent.Since this century, countries in the world are all making great efforts to reduce the application of antibiotic in feed, to reduce the series of human health problem that produces because of the antibiotic improper use.The production of pollution-free food needs more probioticses efficiently.This product is with a wide range of applications.
Four, this product is compound probiotics, and is easy to use.Compound sugar addition in feed is very low, is not easy to mix, and uses this product can overcome this problem.
The specific embodiment
Example 1: the production of composite microorganism type forage additives-Synbiotics preparation
(1) takes xylo-oligosaccharide, the chitosan oligomer that enzyme solution is produced respectively, in cryogenic temperature-20～65 ℃, the lactic acid bacteria bacterium powder of making under vacuum 15～45Pa, Liquid Culture is separated the yeast culture that dry the back, the bacillus subtilis bacterium powder of drying under 40～60 ℃ of temperature under 35～55 ℃ of temperature.
(2) calculate by weight proportion, get xylo-oligosaccharide 0.15Kg, chitosan oligomer 0.58Kg, freeze-dried lactic acid bacteria powder 0.02Kg, saccharomycete culture 0.08Kg, bacillus subtilis 0.45Kg, mix less than 5% the efficient mixer of twin shaft paddle formula with the mixture homogeneity coefficient of variation, package paper is moulded and the clad aluminum foil bag, storage temperature is less than 35 ℃, and storage condition humidity is less than 60%.
Example 2: the production of composite microorganism type forage additives-Synbiotics preparation
Raw material is with example 1.The raw material proportioning is: get xylo-oligosaccharide 0.35Kg, chitosan oligomer 0.15Kg, freeze-dried lactic acid bacteria powder 0.15Kg, saccharomycete culture 0.45Kg, bacillus subtilis 0.03Kg.Method for making is made product with example 1.
Example 3: the production of composite microorganism type forage additives-Synbiotics preparation
Raw material is with example 1.The raw material proportioning is: get xylo-oligosaccharide 0.21Kg, chitosan oligomer 0.30Kg, freeze-dried lactic acid bacteria powder 0.18Kg, saccharomycete culture 0.15Kg, bacillus subtilis 0.18Kg.Method for making is made product with example 1.
Example 4: the production of composite microorganism type forage additives-Synbiotics preparation
Raw material is with example 1.The raw material proportioning is: get xylo-oligosaccharide 0.19Kg, chitosan oligomer 0.021Kg, freeze-dried lactic acid bacteria powder 0.21Kg, saccharomycete culture 0.09Kg, bacillus subtilis 0.20Kg.Method for making is made product with example 1.
Below raise the example of livestock and poultry effect for product of the present invention.
Example 5: meat chick experimental result:
Synbiotics 0.02% interpolation group average weight gain has improved 16.2% than control group, and feedstuff-meat ratio has reduced by 11.3%; 0.01% interpolation group has improved 7.6% than control group, and feedstuff-meat ratio has reduced by 4.4%.Simultaneously, add the morbidity can effectively control fryer by 0.02%, in 42 days, the phenomenon of not suffering from diarrhoea basically, and the incidence of coccidia is low, and the death rate and mortality significantly reduce.
| Testing index | Control group | Synbiotics (0.02%) | Synbiotics (0.01%) |
| Examination chicken number (plumage) | 3000 | 3000 | 3000 |
| Average daily gain (g/ plumage) | 42.17 | 49.04 | 45.39 |
| Feedstuff-meat ratio | 2.04 | 1.81 | 1.95 |
| The dead number (plumage) of washing in a pan | 222 (extremely)+170 (washing in a pan) | 29 (extremely)+139 (washing in a pan) | 160 (extremely)+157 (washing in a pan) |
| Death rate (%) | 13.1 | 5.6 | 10.5 |
Example 6: to the influence of laying hen egg yield
270 age in days left and right sides Hai Lan laying hens, laying rate is 85%, is divided into three groups at random, through formal test in 25 days, the result was as follows:
| Group | Adding proportion | Chicken quantity | Average laying rate | The increase degree |
| 1 | 0.01％ | 2000 | 86.9 | 2.8％ |
| 2 | 0.02％ | 2000 | 90.8 | 7.4％ |
| Contrast | Do not have and add | 2000 | 84.5 |
Example 6: the Synbiotics preparation is to the influence of piglet growth performance and diarrhoea
It is length * pact that 60 kinds are selected in test for use, is in a good state of health 60 of the weanling pigs of body weight basically identical.Stable breeding on the guard rail is freely drunk water, and every day, feeding feed stuff was not as the criterion so that hopper is empty.
| Group | Average daily gain (kg/d) | Average daily ingestion amount (kg/d) | The material anharmonic ratio | Diarrhea rate (%) | The death rate (%) |
| 0.02% adds | 0.483 | 0.730 | 1.512 | 1.48 | 0 |
| 0.01% adds | 0.459 | 0.897 | 1.978 | 2.11 | 7.5％ |
| Control group | 0.318 | 0.816 | 2.753 | 3.45 | 12.0％ |
Example 7: growing and fattening pigs test
Selective body focuses on 90 of tri-crossbreedings about 12kg, at random piggy is divided into three groups.
| Group | Control group | The micro-capsule group | Common group |
| Quantity | 30 | 30 | 30 |
| Average daily gain (g/ head day) | 459 | 548 | 499 |
| The material anharmonic ratio | 2.51 | 2.01 | 2.23 |
| Diarrhea rate (%) | 9.35 | 2.34 | 4.12 |
Claims (5)
1. composite microorganism type forage additives, it is a kind of mixing powder, it is characterized in that forming: 0.10～0.40 part of xylo-oligosaccharide by following components by weight percent, 0.10～0.60 part of chitosan oligomer, 0.01～0.20 part in freeze-dried lactic acid bacteria powder, 0.05～0.50 part of saccharomycete culture, 0.02～0.50 part of bacillus subtilis.
2. according to the described composite microorganism type forage additives of claim 1, it is characterized in that forming: 0.10～0.40 part of xylo-oligosaccharide, 0.20～0.40 part of chitosan oligomer, 0.01～0.05 part in freeze-dried lactic acid bacteria powder, 0.05～0.15 part of saccharomycete culture, 0.02～0.50 part of bacillus subtilis by following components by weight percent.
3. according to the described composite microorganism type forage additives of claim 1, it is characterized in that forming: 0.20 part of xylo-oligosaccharide, 0.20 part of chitosan oligomer, 0.20 part in freeze-dried lactic acid bacteria powder, 0.10 part of saccharomycete culture, 0.20 part of bacillus subtilis by following components by weight percent.
4. the production method of composite microorganism type forage additives is characterized in that raw materials used kind and weight proportion are:
0.10～0.40 part of xylo-oligosaccharide
0.10～0.60 part of chitosan oligomer
0.01～0.20 part in freeze-dried lactic acid bacteria powder
0.05～0.50 part of saccharomycete culture
0.02～0.50 part of bacillus subtilis
Wherein xylo-oligosaccharide, chitosan oligomer are taken the food grade materials that enzyme solution is produced respectively; Lactic acid bacteria powder is made under vacuum 20～60Pa in cryogenic temperature-30～65 ℃; The saccharomycete culture is with 30～70 ℃ of oven dry; Bacillus subtilis is with 40～90 ℃ of oven dry; During production raw material being put into mixer mixes and forms; Pack with paper and clad aluminum foil bag, storage temperature is less than 35 ℃, and storage condition humidity is less than 60%; Described mixer is the mixture homogeneity coefficient of variation less than 5% the efficient mixer of twin shaft paddle formula.
5. according to the production method of the described composite microorganism type forage additives of claim 4, it is characterized in that lactic acid bacteria in cryogenic temperature-20～60 ℃, makes the bacterium powder under vacuum 15～45Pa; Yeast culture is with 35～55 ℃ of oven dry; Bacillus subtilis is with 50～60 ℃ of oven dry.
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| CN104758318B (en) * | 2015-03-06 | 2018-11-30 | 山东宝来利来生物工程股份有限公司 | A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate |
| CN105076708A (en) * | 2015-09-22 | 2015-11-25 | 河南科技大学 | Poultry yeast culture and preparation method thereof |
| CN105248842A (en) * | 2015-10-13 | 2016-01-20 | 武汉农信丰生物科技有限公司 | Preparation method for livestock and poultry feed additive |
| CN107981105A (en) * | 2017-12-07 | 2018-05-04 | 中国热带农业科学院热带生物技术研究所 | A kind of compound immunopotentiator for Babylonia and its feed containing the reinforcing agent |
| CN110447764A (en) * | 2019-07-23 | 2019-11-15 | 张孙林 | A kind of livestock and poultry microbial activity feed addictive and its application method |
| CN110367374B (en) * | 2019-08-28 | 2023-02-28 | 广东省农业科学院蚕业与农产品加工研究所 | Fermented solid material, fermented feed and preparation method thereof |
| CN110692847A (en) * | 2019-11-15 | 2020-01-17 | 河南省科学院生物研究所有限责任公司 | Antibiotic-free mixed feed additive for rabbits |
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