CN106955335A - It is a kind of to prevent Chinese medicine microecological preparation of calf diarrhea and preparation method thereof - Google Patents
It is a kind of to prevent Chinese medicine microecological preparation of calf diarrhea and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a kind of Chinese medicine microecological preparation of prevention calf diarrhea and preparation method thereof, described Chinese medicine microecological preparation includes complex microorganism and Chinese medicine composition, wherein, Chinese medicine composition includes Chinese medicine material：Coloured malt, hawthorn, Divine Comedy, the Radix Astragali, the membrane of a chicken's gizzard, dried orange peel, golden cypress, granatum and wild marjoram；Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, Bacillus cereus and enterococcus faecalis, total viable count >=1 × 10 of complex microorganism10Cfu/g Chinese medicine microecological preparations.The present invention is by after the broken mixing of Chinese medicine material, under the collective effect of complex microorganism, fermentation obtains Chinese medicine microecological preparation, Chinese medicine material active ingredient therein, active material are farthest extracted and utilized, be conducive to the absorption of calf, and the new active material produced after fermentation, the resistance against diseases of calf can be further improved, promotes growing for calf.
Description
Technical field
The invention belongs to pharmaceutical technology field, and in particular to the Chinese medicine microecological preparation and its system of a kind of prevention calf diarrhea
Preparation Method.
Background technology
Calf diarrhea (Calf diarrhea) is a kind of common disease, and the calf of birth -2 months 2 days can occur, dead
Rate is up to 70%, is one of current calf frequently-occurring disease, larger economic loss is brought to cattle-raising.The sick cardinal symptom is diarrhoea,
Excrement is in watery soup or water sample, it may occur that dehydration, acid poisoning, later death rate are higher.The disease using alimentary canal as major transmission path,
Propagate rapidly and easily cause prevalence.It is clinically both at home and abroad to use antibiotic therapy more, but easily produce drug resistance and livestock products medicine
Thing remains excessive problem, harm human and livestock health and public health security.
The problem of part traditional Chinese medical science scholar is directed to calf diarrhea is studied, and develops the Chinese traditional medicine composition of different formulations
Thing, so as to greatly reduce the probability of calf diarrhea, it is to avoid the problem of drug resistance and exceeded medicament residue.But it is existing in
In medicine preparation, there is part Chinese medicine that can the intestines and stomach of calf be caused with certain stimulation and damage, such as cause Flora Disturbance, also can shadow
The human body situation of calf is rung, therefore the application of Chinese medicine composition receives certain limitation.Research and development can have pre- anti-diarrhea, guarantor concurrently
Shield calf intestines and stomach and promote calf grow effect additive turn into stubborn problem.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide it is a kind of prevent calf diarrhea Chinese medicine microecological preparation and
Its preparation method, can promote cud to develop, and adjust ruminal pH value, protect intestines and stomach, suppress enteropahtogenic microganism, reduce abdomen
Rate is rushed down, calf survival rate and daily gain is improved, weans in advance, culture benefit is improved.
To achieve these goals, the technical solution adopted in the present invention is：
A kind of Chinese medicine microecological preparation of prevention calf diarrhea, including complex microorganism and Chinese medicine composition, wherein, Chinese medicine
Composition include following weight than Chinese medicine material：Coloured malt 5-15：Hawthorn 5-15：Divine Comedy 5-15：Radix Astragali 5-15：The membrane of a chicken's gizzard 5-
15：Dried orange peel 5-15：Golden cypress 5-15：Granatum 5-15：Wild marjoram 5-15；
Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy bud
Born of the same parents bacillus and enterococcus faecalis, their number of viable ratio are (2-1)：(2-1)：(2-1)：(2-1)：(2-1)：(2-1)；It is compound micro-
Biological total viable count >=1 × 1010Cfu/g Chinese medicine microecological preparations.
It is preferred that, described Chinese medicine composition include following weight than Chinese medicine material：Coloured malt 10：Hawthorn 10：Divine Comedy
10：The Radix Astragali 10：The membrane of a chicken's gizzard 10：Dried orange peel 10：Golden cypress 10：Granatum 10：Wild marjoram 10.
A kind of preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea, comprises the following steps：
(1) by the lactobacillus bulgaricus of preservation, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy brood cell's bar
The bacterium solution of bacterium and enterococcus faecalis is inoculated in respective fluid nutrient medium with 1 ‰ inoculum concentration respectively, is shaken under the conditions of 38 DEG C
Overnight incubation, rotating speed is 200rpm；
(2) weigh each Chinese medicine material by weight, and be well mixed after each Chinese medicine material is crushed respectively, as solid
Body base-material；
(3) ratio of 20ml nutrient solutions is added according to every 100g solid bases, by solid base and step (1) each strain
Nutrient solution is mixed respectively, and is stirred；
(4) by step (3) each bacteria culture fluid and the mixture of the solid base closed culture 24h under the conditions of 38 DEG C respectively
More than, and determining the number of viable of each microbial strain culture, it is ensured that the number of viable of each microbial strain culture meets Traditional Chinese drug microecology system
Agent viable bacteria requires；
(5) each microbial strain culture of step (4) is dried into 48h under the conditions of 45 DEG C respectively, crushes packing, each strain is made
Preparation；
(6) preparation of each strain is mixed by the number of viable ratio of each strain, Chinese medicine microecological preparation is made, and ensure to live
Bacterium sum >=1 × 1010Cfu/g Chinese medicine microecological preparations.
The fluid nutrient medium of described lactobacillus bulgaricus is：Peptone 10g, beef extract 10g, yeast extract 5g, K2HPO4
2g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween 80 1ml, Mg SO4·7H2O 0.25g、Mn SO4·4H2O
0.58g, distilled water 1000ml.
The fluid nutrient medium of described streptococcus thermophilus is：Whey 500ml, distilled water 500ml, tryptone 5g, grape
Sugared 1g, yeast extract 2.5g, agar 20g, pH 6.5.
The fluid nutrient medium of described Bifidobacterium is：Tryptone 5g, soy peptone 5g, dusty yeast 10g, glucose
10g, Tween 80 1ml, agar 15-20g, L-cysteine hydrochloride 0.05g, salting liquid 4ml, distilled water 1000ml；Described
Salting liquid is：CaCl2 0.2g、K2HPO4 1.0g、KH2PO4 1.0g、MgSO4·7H2O 0.48g、Na2CO3 10g、NaCl
2g, distilled water 1000ml.
Described bacillus subtilis and the fluid nutrient medium of Bacillus cereus are：Tryptone 10g, beef extract 3g,
Sodium chloride 5g, distilled water 1000ml, pH7.4.
The fluid nutrient medium of described enterococcus faecalis is：Tryptone 10g, beef extract 10g, dusty yeast 5g, K2HPO4 2g、
Dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1ml, MgSO4·7H2O 0.58g、MnSO4·4H2O
0.25g, distilled water 1000ml.
Coloured malt, cures mainly dyspepsia, and abdominal distention, spleen eating less, newborn sweat smoulders, swollen breasts, women weans.
Hawthorn, sour sweet, slightly warm in nature, enters spleen, stomach, Liver Channel.Major function：Digestion gathered food, scattered blood stasis, expelling tenia.Meat product is controlled,
Abdominal mass, phlegm and retained fluid, feeling of fullness, acid regurgitation, rush down dysentery, intestines wind, pain in the back, hernia postpartum postpartum abdominal pain, lochiorrhagia, pediatric milk food stagnation.Help digestion
Stomach invigorating, scattered stasis.For meat stagnation, gastric ulcer fullness, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, confidant sting, hernia
Pain, hyperlipemia.
Divine Comedy, it is sweet, pungent, it is warm-natured.Returns spleen, stomach.Major function：Help digestion and stomach.Cure mainly eating accumulation, abdominal fullness and distention,
Deficiency of food is indigestion and loss of appetite.
The Radix Astragali, sweet, slightly warm in nature.Return lung, spleen, liver and kidney channel.Major function：Exterior deficiency spontaneous perspiration, night sweat due to yin deficiency, acute and chronic kidney
Scorching oedema, yang-energy weakness, sore diabrosis, syndrome of deficiency of lung qi, the deficiency of vital energy are weak.
The membrane of a chicken's gizzard, it is sweet, it is mild-natured.Returns spleen, stomach, small intestine, bladder warp.Major function：Stomach strengthening and digestion promoting, arresting seminal emission is treating stranguria
Fossil.
Dried orange peel, it is bitter, pungent, it is warm-natured.Return lung, the spleen channel.Major function：Regulating qi-flowing for strengthening spleen is eliminating dampness and eliminating phlegm.For abdominal fullness and distention,
Deficiency of food is vomited and diarrhoea, coughing with a lot of sputum.
Golden cypress, bitter is cold in nature.Return kidney, bladder warp.Major function：Heat-clearing and damp-drying drug, purging intense heat, except steaming, detoxification sore treatment.For wet
Hot rush down dysentery, jaundice, with lower pruritus vulvae, puckery pain of heat gonorrhea, tinea pedis impotence is knitted, and hectic fever due to yin labor heat, night sweat, seminal emission, sore swollen toxin, eczema is wet
Sore.Stir-baked CORTEX PHELLODENDRI with salt solution nourishing Yin and falling fire.For fire excess from yin deficiency, night sweat hectic fever due to yin.
Granatum, it is sour, puckery, it is warm-natured.Return large intestine channel.Major function：Relieving diarrhea with astringents, hemostasis, expelling parasite.For chronic diarrhea, long
Dysentery, has blood in stool, prolapse of the anus, uterine bleeding, under band, abdominal pain due to enterositosis.
Wild marjoram, it is pungent, it is cool in nature.Major function：Inducing sweat and dispelling exogenous evils, dampness elimination of relieving summer heat.For heatstroke, flu, acute gastroenteritis, abdomen
Bitterly.
Beneficial effects of the present invention：
1st, the characteristics of present invention is not likely to produce drug resistance and livestock products medicament residue using Chinese medicine, selects the stomach and intestine to calf
Substantially non-stimulated and damage the Chinese medicine material coloured malt in road, hawthorn, Divine Comedy, the Radix Astragali, the membrane of a chicken's gizzard, dried orange peel, golden cypress, granatum, ox
Scientific compatibility is carried out to waiting, using its synergy each other, can effectively suppress enteropahtogenic microganism, reduce calf
Diarrhea rate, promotes cud development, adjusts ruminal pH value, improve calf survival rate and daily gain, wean in advance, improves cultivation effect
Benefit.
2nd, complex microorganism of the invention is another innovative point of the present invention, and the present invention is closed different microorganisms
Reason proportioning, can further suppress the growth of enteropahtogenic microganism, improve beneficial microorganism quantity in enteron aisle, reduce calf
Diarrhea rate, while the addition of complex microorganism, can adjust the microenvironment in intestines and stomach, adjusts ruminal pH value, promotes cud hair
Educate, protect gastrointestinal health, promote absorption of the intestines and stomach to nutritional ingredient, vitamin and mineral matter, promote the growth hair of calf
Educate.
3rd, the present invention is by after the broken mixing of Chinese medicine material, in lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, withered
Under the collective effect of careless bacillus, Bacillus cereus and enterococcus faecalis, fermentation obtains Chinese medicine microecological preparation, it is therein in
Medicine material effective component, active material are farthest extracted and utilized, and are conducive to what is produced after the absorption of calf, and fermentation
New active material, can further improve the resistance against diseases of calf, promote growing for calf.The product fusion of the present invention
The materials such as Chinese herbal medicine active component, beneficial microbe, and carried out fermentation optimization on this basis, realize Chinese medicine and micro- life
The maximization that thing is utilized, with good social and economic benefit.
Embodiment
The embodiment to the present invention is described in further detail with reference to embodiments.
Fluid nutrient medium used by present invention culture strain can be conventional fluid nutrient medium, can also voluntarily prepare,
Specifically, the fluid nutrient medium used by the present invention is as follows：
The fluid nutrient medium of lactobacillus bulgaricus is：Peptone 10g, beef extract 10g, yeast extract 5g, K2HPO42g, vinegar
Sour sodium 5g, ammonium citrate 2g, glucose 20g, Tween 80 1ml, Mg SO4·7H2O 0.25g、Mn SO4·4H2O 0.58g、
Distilled water 1000ml.
The fluid nutrient medium of streptococcus thermophilus is：Whey 500ml, distilled water 500ml, tryptone 5g, glucose 1g, ferment
Female cream 2.5g, agar 20g, pH 6.5.
The fluid nutrient medium of Bifidobacterium is：Tryptone 5g, soy peptone 5g, dusty yeast 10g, glucose 10g, tell
80 1ml of temperature, agar 15-20g, L-cysteine hydrochloride 0.05g, salting liquid 4ml, distilled water 1000ml；Described salting liquid
For：CaCl2 0.2g、K2HPO4 1.0g、KH2PO4 1.0g、MgSO4·7H2O 0.48g、Na2CO310g, NaCl 2g, distillation
Water 1000ml.
The fluid nutrient medium of bacillus subtilis and Bacillus cereus is：Tryptone 10g, beef extract 3g, sodium chloride
5g, distilled water 1000ml, pH7.4.
The fluid nutrient medium of enterococcus faecalis is：Tryptone 10g, beef extract 10g, dusty yeast 5g, K2HPO42g, citric acid
Two ammonium 2g, sodium acetate 5g, glucose 20g, Tween 80 1ml, MgSO4·7H2O 0.58g、MnSO4·4H2O 0.25g, distillation
Water 1000ml.
Embodiment 1
A kind of Chinese medicine microecological preparation of prevention calf diarrhea, including complex microorganism and Chinese medicine composition, wherein, Chinese medicine
Composition include following weight than Chinese medicine material：Coloured malt 10：Hawthorn 10：Divine Comedy 10：The Radix Astragali 10：The membrane of a chicken's gizzard 10：Dried orange peel 10：
Golden cypress 10：Granatum 10：Wild marjoram 10.
Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy bud
Born of the same parents bacillus and enterococcus faecalis, their number of viable ratio are 1：1：1：1：1：1；Total viable count >=1 of complex microorganism ×
1010Cfu/g Chinese medicine microecological preparations.
The preparation method of the Chinese medicine microecological preparation of described prevention calf diarrhea, comprises the following steps：
(1) by the lactobacillus bulgaricus of preservation, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy brood cell's bar
The bacterium solution of bacterium and enterococcus faecalis is inoculated in respective fluid nutrient medium with 1 ‰ inoculum concentration respectively, is shaken under the conditions of 38 DEG C
Overnight incubation, rotating speed is 200rpm；
(2) weigh each Chinese medicine material by weight, and be well mixed after each Chinese medicine material is crushed respectively, as solid
Body base-material；
(3) ratio of 20ml nutrient solutions is added according to every 100g solid bases, by solid base and step (1) each strain
Nutrient solution is mixed respectively, and is stirred；
(4) by step (3) each bacteria culture fluid and the mixture of the solid base closed culture 24h under the conditions of 38 DEG C respectively
More than, and determining the number of viable of each microbial strain culture, it is ensured that the number of viable of each microbial strain culture meets Traditional Chinese drug microecology system
Agent viable bacteria requires；
(5) each microbial strain culture of step (4) is dried into 48h under the conditions of 45 DEG C respectively, crushes packing, each strain is made
Preparation；
(6) preparation of each strain is mixed by the number of viable ratio of each strain, Chinese medicine microecological preparation is made, and ensure to live
Bacterium sum >=1 × 1010Cfu/g Chinese medicine microecological preparations.
Embodiment 2
A kind of Chinese medicine microecological preparation of prevention calf diarrhea, including complex microorganism and Chinese medicine composition, wherein, Chinese medicine
Composition include following weight than Chinese medicine material：Coloured malt 15：Hawthorn 5：Divine Comedy 15：The Radix Astragali 5：The membrane of a chicken's gizzard 5：Dried orange peel 5：Golden cypress
15：Granatum 5：Wild marjoram 5.
Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy bud
Born of the same parents bacillus and enterococcus faecalis, their number of viable ratio are 2：2：1：1：1：2；Total viable count >=1 of complex microorganism ×
1010Cfu/g Chinese medicine microecological preparations.
The preparation method be the same as Example 1 of described Chinese medicine microecological preparation.
Embodiment 3
A kind of Chinese medicine microecological preparation of prevention calf diarrhea, including complex microorganism and Chinese medicine composition, wherein, Chinese medicine
Composition include following weight than Chinese medicine material：Coloured malt 5：Hawthorn 15：Divine Comedy 5：The Radix Astragali 15：The membrane of a chicken's gizzard 15：Dried orange peel 15：It is yellow
Cypress 5：Granatum 15：Wild marjoram 15.
Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy bud
Born of the same parents bacillus and enterococcus faecalis, their number of viable ratio are 1：1：1：2：2：2；Total viable count >=1 of complex microorganism ×
1010Cfu/g Chinese medicine microecological preparations.
The preparation method be the same as Example 1 of described Chinese medicine microecological preparation.
Application test
1st, test material
Animal experiment is carried out in certain pasture, from body weight is close, be born 10 age in days calf 180, is randomly divided into 6 groups, examination
A group 1-3, control group 1-2 and blank control group are tested, every group of 3 repetitions each repeat 10 calves.Test group 1-3 is respectively in ox
The embodiment 1-3 of addition 2% Chinese medicine microecological preparation and supplementary feeding particulate material in milk, control group 1 is added in milk with implementation
The complex microorganism and supplementary feeding particulate material of example 1, control group 2 add the Chinese medicine composition and benefit of 2% be the same as Example 1 in milk
Particulate material is raised, blank control group only feeds milk and supplementary feeding particulate material.The addition of supplementary feeding particulate material is conventional scale of feeding, allows calf
Ox free choice feeding, feeds duty and adds less.
2nd, feeding and management
Experimental period common 50d, during which calf free choice feeding, free water.Experimentation by pasture requirement carry out hygienic cleaning,
The daily managements such as epidemic prevention, colony house sterilization.
3rd, project and method are determined
(1) daily gain.1st day morning claimed experiment calf empty body weight as original body mass before raising by head, during off-test
Empty body weight records to calculate average daily gain, daily gain=(weight-starting weight eventually)/number of days as whole body weight.
(2) diarrhea disease percentage.Calculate in experimental period, the diarrhoea incidence of the experiment calf of different groups.
4th, result and analysis
Table 1：Different additive is to calf daily gain, the influence of diarrhea rate
As can be seen from Table 1：Test group and control group are respectively provided with the effect of prevention calf diarrhea, but the effect of test group
Control group is substantially better than, and the additive of test group promotes absorption of the animal intestinal tract to nutriment to greatest extent, significantly carries
The high daily gain of calf.
The optimal embodiment of the present invention is the foregoing is only, for a person skilled in the art, the present invention can have
Various modifications and variations.Within the spirit and principles of the invention, any modifications, equivalent substitutions and improvements made etc., all should
Within protection scope of the present invention.
Claims (8)
1. a kind of Chinese medicine microecological preparation of prevention calf diarrhea, it is characterised in that including complex microorganism and Chinese medicine composition,
Wherein, Chinese medicine composition include following weight than Chinese medicine material：Coloured malt 5-15：Hawthorn 5-15：Divine Comedy 5-15：Radix Astragali 5-
15：The membrane of a chicken's gizzard 5-15：Dried orange peel 5-15：Golden cypress 5-15：Granatum 5-15：Wild marjoram 5-15；
Complex microorganism includes lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, waxy brood cell's bar
Bacterium and enterococcus faecalis, their number of viable ratio are (2-1)：(2-1)：(2-1)：(2-1)：(2-1)：(2-1)；Complex microorganism
Total viable count >=1 × 1010Cfu/g Chinese medicine microecological preparations.
2. the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 1, it is characterised in that described Chinese medicine group
Compound include following weight than Chinese medicine material：Coloured malt 10：Hawthorn 10：Divine Comedy 10：The Radix Astragali 10：The membrane of a chicken's gizzard 10：Dried orange peel 10：It is yellow
Cypress 10：Granatum 10：Wild marjoram 10.
3. a kind of preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea as claimed in claim 1 or 2, its feature exists
In comprising the following steps：
(1) by the lactobacillus bulgaricus of preservation, streptococcus thermophilus, Bifidobacterium, bacillus subtilis, Bacillus cereus and
The bacterium solution of enterococcus faecalis is inoculated in respective fluid nutrient medium with 1 ‰ inoculum concentration respectively, and culture is shaken under the conditions of 38 DEG C
Overnight, rotating speed is 200rpm；
(2) weigh each Chinese medicine material by weight, and be well mixed after each Chinese medicine material is crushed respectively, be used as solid-based
Material；
(3) ratio of 20ml nutrient solutions is added according to every 100g solid bases, by the culture of solid base and step (1) each strain
Liquid is mixed respectively, and is stirred；
(4) by step (3) each bacteria culture fluid and the mixture of solid base respectively under the conditions of 38 DEG C closed culture 24h with
On, and determining the number of viable of each microbial strain culture, it is ensured that the number of viable of each microbial strain culture meets Chinese medicine microecological preparation
Viable bacteria requires；
(5) each microbial strain culture of step (4) is dried into 48h under the conditions of 45 DEG C respectively, crushes packing, the system of each strain is made
Agent；
(6) preparation of each strain is mixed by the number of viable ratio of each strain, Chinese medicine microecological preparation is made, and ensure that viable bacteria is total
Number >=1 × 1010Cfu/g Chinese medicine microecological preparations.
4. the preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 3, it is characterised in that institute
The fluid nutrient medium for the lactobacillus bulgaricus stated is：Peptone 10g, beef extract 10g, yeast extract 5g, K2HPO42g, sodium acetate
5g, ammonium citrate 2g, glucose 20g, Tween 80 1ml, Mg SO4·7H2O 0.25g、Mn SO4·4H2O 0.58g, distillation
Water 1000ml.
5. the preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 3, it is characterised in that institute
The fluid nutrient medium for the streptococcus thermophilus stated is：Whey 500ml, distilled water 500ml, tryptone 5g, glucose 1g, yeast extract
2.5g, agar 20g, pH 6.5.
6. the preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 3, it is characterised in that institute
The fluid nutrient medium for the Bifidobacterium stated is：Tryptone 5g, soy peptone 5g, dusty yeast 10g, glucose 10g, Tween 80
1ml, agar 15-20g, L-cysteine hydrochloride 0.05g, salting liquid 4ml, distilled water 1000ml；Described salting liquid is：
CaCl2 0.2g、K2HPO4 1.0g、KH2PO41.0g、MgSO4·7H2O 0.48g、Na2CO310g, NaCl 2g, distilled water
1000ml。
7. the preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 3, it is characterised in that institute
The bacillus subtilis stated and the fluid nutrient medium of Bacillus cereus are：Tryptone 10g, beef extract 3g, sodium chloride 5g, steaming
Distilled water 1000ml, pH7.4.
8. the preparation method of the Chinese medicine microecological preparation of prevention calf diarrhea according to claim 3, it is characterised in that institute
The fluid nutrient medium for the enterococcus faecalis stated is：Tryptone 10g, beef extract 10g, dusty yeast 5g, K2HPO42g, dibasic ammonium citrate
2g, sodium acetate 5g, glucose 20g, Tween 80 1ml, MgSO4·7H2O 0.58g、MnSO4·4H2O 0.25g, distilled water
1000ml。
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| CN110771744A (en) * | 2019-11-14 | 2020-02-11 | 中国农业科学院北京畜牧兽医研究所 | Preparation method of feed for preventing early weaning diarrhea of calves |
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| CN111671003A (en) * | 2020-07-22 | 2020-09-18 | 内蒙古自治区农牧业科学院 | Antibacterial fattening feed additive and preparation and use methods thereof |
| CN112426501A (en) * | 2020-12-03 | 2021-03-02 | 河南中医药大学 | Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof |
| CN114159502A (en) * | 2021-11-01 | 2022-03-11 | 齐鲁工业大学 | Anti-diarrhea traditional Chinese medicine composition fermented by bacillus cereus, preparation method and application |
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| CN115216429A (en) * | 2022-07-06 | 2022-10-21 | 安徽天安生物科技股份有限公司 | Composite microbial agent prepared based on hawthorn aqueous extract and preparation method and application thereof |
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| CN110771744A (en) * | 2019-11-14 | 2020-02-11 | 中国农业科学院北京畜牧兽医研究所 | Preparation method of feed for preventing early weaning diarrhea of calves |
| CN111557382A (en) * | 2020-05-22 | 2020-08-21 | 开封嘉骏生物科技有限公司 | Functional additive for preventing milk accumulation and diarrhea of calves and lambs and preparation method thereof |
| CN111671003A (en) * | 2020-07-22 | 2020-09-18 | 内蒙古自治区农牧业科学院 | Antibacterial fattening feed additive and preparation and use methods thereof |
| CN112426501A (en) * | 2020-12-03 | 2021-03-02 | 河南中医药大学 | Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof |
| CN112426501B (en) * | 2020-12-03 | 2022-04-15 | 河南中医药大学 | Traditional Chinese medicine microecological preparation for promoting growth and preventing diarrhea of calves and preparation method thereof |
| CN114949127A (en) * | 2020-12-31 | 2022-08-30 | 河南牧业经济学院 | Traditional Chinese medicine microecological preparation for treating calf diarrhea |
| CN114949127B (en) * | 2020-12-31 | 2023-04-28 | 河南牧业经济学院 | Traditional Chinese medicine microecological preparation for treating calf diarrhea |
| CN114159502A (en) * | 2021-11-01 | 2022-03-11 | 齐鲁工业大学 | Anti-diarrhea traditional Chinese medicine composition fermented by bacillus cereus, preparation method and application |
| CN114159502B (en) * | 2021-11-01 | 2022-11-08 | 齐鲁工业大学 | Anti-diarrhea traditional Chinese medicine composition fermented by bacillus cereus, preparation method and application |
| CN115216429A (en) * | 2022-07-06 | 2022-10-21 | 安徽天安生物科技股份有限公司 | Composite microbial agent prepared based on hawthorn aqueous extract and preparation method and application thereof |
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