CN101028037A - Feed additive for improving fowl and beast entric immune and its production - Google Patents
Feed additive for improving fowl and beast entric immune and its production Download PDFInfo
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Abstract
A forage additive for improving intestinal immunity of fowls and animals is proportionally prepared from the powdered Bacillus cereus, powdered caseous lactobacillus, powdered uroenterococcus and powdered saccharomyces cerevisine through respective fermenting, centrifugal separation, drying at 50 deg.C or freeze-drying, and proportionally mixing them with corn powder or wheat bran.
Description
Technical field: the present invention relates to be used to improve fowl poultry intestinal microecology balance, feed addictive that the probio of enhancing livestock immunity and raising efficiency of feed utilization is made and the technology for preparing this additive.
Background technology: the research of existing probio also is only limited to the research to single bacterial classification, not at beneficial flora mutually promote, symbiosis, coordinate to promote to improve fowl poultry intestine immunity power, also do not coordinate to promote to improve the beneficial flora feed addictive and the technology of fowl poultry intestine immunity power.
Summary of the invention:
The present invention will solve problem how to utilize beneficial flora to coordinate to promote to improve fowl poultry intestine immunity power, mainly be utilize isolate in the animal body four kinds of probiotics strains with physiological action through the fermentation separating thallus, dryly be mixed into probiotic feed additive through reasonable prescription, provide simultaneously a kind of probiotic feed additive manufacturing process.
Probiotic feed additive of the present invention takes by weighing Bacillus cereus bacterium powder 5-15 part by weight, Lactobacillus casei bacterium powder 5-15 part, and VREF 5-15 part, saccharomyces cerevisiae powder 2000-3000 part mixes.Can also add 10000 parts-20000 parts in corn flour or wheat bran powder.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 200-500 hundred million/g.Additive and feed ratio are 1: 1000.Be that every kg probio additive can be prepared the 1000-2000kg feed (0.2-0.5 hundred million/g).
Probiotic feed additive of the present invention manufacturing process.Take by weighing Bacillus cereus bacterium powder 5-15 part by weight, Lactobacillus casei bacterium powder 5-15 part, VREF 5-15 part, saccharomyces cerevisiae powder 2000-3000 part, corn flour or wheat bran powder 10000-20000 part.The probio additive of making by above prescription.Every gram contains profitable probliotics and counts 200-500 hundred million/g.Additive and feed ratio are 1: 1000.Be that every kg Yishengkang additive can be prepared the 1000-2000kg feed (0.2-0.5 hundred million/g).Wherein, single bacterium powder preparation technique is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 5-10% bean sprouts juice, 0.2-0.5% glucose, the 0.5-1.5% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 2-4 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2-4 hour, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 1%-3% glucose, 0.5-1.5% peptone, the 0.5-1% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25-40% in the sodium glutamate that contains weight 3-5% and the 5-15% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 1%-3% glucose, 0.5-1.5% peptone, 0.5-1% yeast extract, 0.1-0.5%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.1-0.5%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25-40% in the sodium glutamate that contains 3-5%, the 5-15% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 5-10% bean sprouts juice, 0.2-0.5% glucose, the 0.5-1.5% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 2-4 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2-4 hour, can obtain S. cervisiae bacterium powder.
Advantage of the present invention: 4 kinds of bacterium are colonizated in enteron aisle upper, middle and lower position respectively, can suppress breeding and the invasion of the harmful bacterium in the whole enteron aisle, remove each harmful substance of enteron aisle, and opposing is infected, and safeguards the archenteric flora balance.Each bacterial classification can both be grown under different condition one of enteron aisle different parts composition, acts on fast and lasting associating flora, forms one biological barrier on whole intestinal mucosa surface, prevents the growth and breeding of pathogenic bacteria conditioned pathogen, removes endotoxin.4 kinds of bacterium are united use, and the growth characteristic of each bacterial classification is different with function, have general character that specificity is also arranged, and the effect of many synergistic bacteriums can make it perform to the best, suitable to greatest extent all kinds of host animals.Four kinds of probios of the present invention are by the research of toxicology and pharmacology test, prove all safety and having no side effect of each bacterial strain.
We are by studying shown in table 1, table 2, table 3 Bacillus cereus and Lactobacillus casei and VREF symbiosis, the result shows: Bacillus cereus all has the growth of promotion effect to Lactobacillus casei and VREF under the certain hour condition in two kinds of different nutrient solutions.It is less to influence each other between two bacterial strains during two kinds of bacterium Mixed culture of Lactobacillus casei and VREF.
Table 1 Bacillus cereus and Lactobacillus casei are at different culture mediums and training method growing state (X ± S, log10
n/ g, n=5)
| Culture medium | Cultivation | Bacterial classification | 0 hour | 12 hours | 24 hours | 48 hours | 72 hours |
| Lbs | Cultivate separately | Lactobacillus casei | 4.52±0.37 | 7.51±0.34 | 9.12±0.34 | 8.66±0.33 | 8.21±0.43 |
| Cultivate separately | Bacillus cereus | 3.35±0.22 | 5.88±0.44 | 6.91±0.46 | 6.54±0.26 | 6.23±0.35 | |
| Mixed culture | Lactobacillus casei | 4.32±0.46 | 8.22±0.45 | 9.55±0.55 | 9.67±0.26 | 9.25±0.56 | |
| Bacillus cereus | 3.32±0.45 | 5.65±0.44 | 5.78±0.47 | 5.21±0.38 | 5.12±0.39 | ||
| Nb | Cultivate separately | Lactobacillus casei | 4.11±0.27 | 6.77±0.66 | 8.39±0.82 | 8.06±0.44 | 7.88±0.33 |
| Cultivate separately | Bacillus cereus | 3.23±0.39 | 7.55±0.32 | 8.13±0.25 | 8.02±0.46 | 7.45±0.57 | |
| Mixed culture | Lactobacillus casei | 4.67±0.44 | 7.93±0.37 | 9.53±0.44 | 9.24±0.86 | 8.57±0.38 | |
| Bacillus cereus | 3.44±0.57 | 6.56±0.58 | 7.03±0.11 | 5.89±0.48 | 5.78±0.29 |
Table 2 Bacillus cereus and VREF be two bacteria growing situation (X ± S, log10 when different culture mediums and training method
n/ g, n=5)
| Culture medium | Cultivation | Bacterial classification | 0 hour | 12 hours | 24 hours | 48 hours | 72 hours |
| Ec | Cultivate separately | VREF | 5.35±0.11 | 7.12±0.55 | 8.36±0.12 | 8.22±0.45 | 8.12±0.61 |
| Cultivate separately | Bacillus cereus | 3.52±0.46 | 7.44±0.67 | 7.88±0.32 | 7.43±0.74 | 7.78±0.43 | |
| Mixed culture | VREF | 5.35±0.11 | 8.78±0.34 | 8.88±0.23 | 8.68±0.45 | 8.45±0.37 | |
| Bacillus cereus | 3.45±0.45 | 5.78±0.44 | 5.88±0.79 | 5.41±0.65 | 5.02±0.78 | ||
| Nb | Cultivate separately | VREF | 5.35±0.11 | 6.89±0.12 | 8.22±0.92 | 8.01±0.28 | 7.26±0.55 |
| Cultivate separately | Bacillus cereus | 3.23±0.56 | 7.88±0.72 | 8.63±0.66 | 8.32±0.32 | 7.25±0.59 | |
| Mixed culture | VREF | 5.35±0.11 | 7.68±0.35 | 8.55±0.34 | 8.23±0.64 | 7.95±0.55 | |
| Bacillus cereus | 3.44±0.57 | 6.56±0.58 | 7.03±0.11 | 5.89±0.48 | 5.78±0.29 |
Table 3 Lactobacillus casei and VREF are at different culture mediums and different training method growing state (X ± S, log10
n/ g, n=5)
| Culture medium | Cultivation | Bacterial classification | 0 hour | 12 hours | 24 hours | 48 hours | 72 hours |
| Lbs | Cultivate separately | Lactobacillus casei | 4.52±0.37 | 7.51±0.34 | 9.02±0.44 | 8.56±0.23 | 8.27±0.54 |
| Cultivate separately | VREF | 4.34±0.39 | 6.57±0.67 | 8.02±0.35 | 8.12±0.67 | 8.01±0.58 | |
| Mixed culture | Lactobacillus casei | 4.72±0.38 | 7.35±0.75 | 7.99±0.30 | 8.46±0.60 | 7.57±0.88 | |
| VREF | 4.38±0.21 | 6.77±0.35 | 8.16±0.39 | 8.00±0. | 7.97±0.94 | ||
| Ec | Cultivate separately | Lactobacillus casei | 4.62±0.58 | 6.98±0.46 | 8.54±0.67 | 8.21±0.33 | 8.01±0.24 |
| Cultivate separately | VREF | 4.38±0.61 | 7.42±0.35 | 8.46±0.62 | 8.21±0.45 | 8.12±0.64 | |
| Mixed culture | Lactobacillus casei | 4.67±0.31 | 6.79±0.77 | 8.44±0.66 | 8.35±0.45 | 8.12±0.35 | |
| VREF | 4.54±0.29 | 7.30±0. | 8.01±0.40 | 7.69±0.60 | 7.89±0.90 |
Cooperate us to probiotics strain involved in the present invention separately or unite use by cooperation unit, kind of a chicken intestinal flora, ight soil pH, harmful substance ammonia content and hydrogen sulfide content, water content and production performance are studied, see Table 4, table 5, table 6.The result shows: the zoology test by 5 groups of different probiotics preparations that kind of chicken is fed is observed, profitable strain (Bifidobacterium, lactobacillus) and pernicious bacteria staphylococcus, enterococcus, enterobacteria, clostridium in Toxic in the chicken intestines (sulfide, ammonia) and organic acid (lactic acid, acetate, butyric acid, isobutyric acid) the chicken intestines), parameter such as intestinal contents moisture compares, the result shows that to adopt 4 kinds of bacterium mix preparations more effective than Lactobacillus casei and bacillus cereus two bacterium mix preparations and Lactobacillus casei or the single bacteria preparation of bacillus cereus.And to comparing the production performance aspect of laying hen, four bacterium mix preparations are better than two bacterium mix preparations and single bacteria preparation.Do not have between 4 groups of bacteria preparations and the contrast post significantly to be inserted in and produce and the hatching aspect of performance does not have significant difference.
The 30th day kind chicken intestinal contents physical and chemical index situation of table 4 test (X ± S, n=6)
| Grouping | A | B | C | D | E |
| PH moisture (%) ammonia (mg/g) hydrogen sulfide (mg/g) lactic acid (mmol/g) acetic acid (mmol/g) butyric acid (mmol/g) isobutyric acid (mmol/g) | 7.25±0.32 75.49±3.54 2.33±1.53 1.02±0.65 0.21±0.10 0.38±0.09 0.17±0.12 0.08±0.03 | 7.41±0.32 76.45±4.21 1.49±1.46 0.67±0.67 0.22±0.18 0.58±0.14 0.06±0.03 0.07±0.02 | 6.39±0.44 77.44±3.95 0.83±0.47 0.67±0.75 0.31±0.03 1.45±0.21 0.14±0.05 0.08±0.02 | 6.57±0.35 77.29±3.39 0.84±0.98 0.38±0.55 0.48±0.04 1.85±0.75 0.09±0.04 0.06±0.03 | 7.68±0.72 77.64±3.28 3.45±0.91 3.78±1.25 0.09±0.04 0.28±0.13 0.05±0.02 0.10±0.02 |
Annotate: test is divided into 5 groups, and every group of 100 chickens are separately raised.A, B, C, D, be experimental group, the E group is control group.Measured every index on the 30th day respectively in test.A group: the Bacillus cereus bacterium powder that in feed, adds 0.4 hundred million/g; B group: the Lactobacillus casei bacterium powder that in feed, adds 0.4 hundred million/g; C group: in feed, add Bacillus cereus bacterium powder and the Lactobacillus casei bacterium powder that contains 0.2 hundred million/g respectively; D group: in feed, add Bacillus cereus bacterium powder, Lactobacillus casei bacterium powder, VREF bacterium powder and the S. cervisiae powder that contains 0.1 hundred million/g respectively; E group: the conventional feed of feeding.
The 30th day kind chicken intestinal flora situation of table 5 test
| Grouping | Staphylococcus | Enterococcus | Escherichia coli | Lactobacillus | Bifidobacterium | Perfringens bacillus |
| A B C D E | 7.04±0.29 7.83±0.06 7.37±0.67 7.23±0.34 8.52±0.30 | 5.60±0.40 6.15±0.44 6.03±0.28 5.69±0.45 7.84±0.31 | 6.23±0.21 6.01±0.34 5.33±0.45 5.21±0.70 6.91±0.18 | 8.07±0.38 8.61±0.26 8.33±0.42 8.81±0.52 7.07±0.76 | 8.76±0.21 8.43±0.23 8.72±0.23 8.89±0.19 7.80±0.13 | 5.04±0.54 5.29±0.24 4.69±0.55 4.32±0.54 7.35±0.68 |
Chicken production performance performance condition is planted in table 6 test after the 30th day
| Index | A | B | C | D | E |
| Survival rate (%) laying rate (%) day per unit area yield (g) feedstuff-egg ratio rate of fertilization (%) | 98 90.3 49.5 1∶2.15 96.9 | 97 88.3 50.3 1∶2.15 97.8 | 99 93.5 51.4 1∶2.17 97.1 | 99 90.8 52.3 1∶2.15 96.4 | 97 91.2 50.2 1∶2.16 96.8 |
| Hatchability of fertile eggs (%) | 90.4 | 91.1 | 89.5 | 89.7 | 90.2 |
We carry out immune function of mice research to bacillus cereus, Lactobacillus casei and two kinds of bacterium mix preparations respectively, and result of study shows that the immunoregulation effect index of the mix preparation of Lactobacillus casei and bacillus cereus is higher than the unitary agent of Lactobacillus casei and bacillus cereus respectively.The results are shown in Table 7.
Several probiotics preparations of table 7 are to effect of immunologic function
| Index | A | B | C | D | E |
| Have a liking for cytophagy rate (%) to huge | 26.65±0.87 | 27.23±0.56 | 31.42±0.89 | 36.12±0.88 | 22.13±0.64 |
| Phagocytic index | 0,65±0.02 | 0.69±0.03 | 0.72±0.04 | 0.81±0.04 | 0.62±0.04 |
| The mouse hemolysin is tired | 1∶200 | 1∶240 | 1∶260 | 1∶300 | 1∶120 |
| NK cytoactive (%) | 19.32±7.67 | 18.53±5.67 | 20.24±4.78 | 28.34±4.12 | 15.31±6.23 |
Annotate: test is divided into 5 groups, every group of 10 BALB/c mouses, oral administration gavage, grouping administration.A, B, C, D, be experimental group, the E group is control group.Measured every index on the 15th day respectively in test.A group: the Bacillus cereus bacterium powder that is equivalent to irritate stomach 1,000,000,000/kg; B group: the Lactobacillus casei bacterium powder that is equivalent to irritate stomach 1,000,000,000/kg; C group: be equivalent to irritate Bacillus cereus bacterium powder and the Lactobacillus casei bacterium powder that stomach contains 500,000,000/kg respectively; D group: be equivalent to irritate Bacillus cereus bacterium powder, Lactobacillus casei bacterium powder, VREF bacterium powder and the S. cervisiae powder that stomach contains 2.5/kg respectively; E group: irritate stomach 10ml/kg physiological saline.
The specific embodiment:
Embodiment 1 Bacillus cereus bacterium powder 6g, Lactobacillus casei bacterium powder 10g, VREF 14g, saccharomyces cerevisiae powder 3000g, corn flour or wheat bran powder 15kg.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 200-500 hundred million/g.Additive and feed ratio are 1: 1000.Be that every kg Yishengkang additive can be prepared the 1000-2000kg feed (0.2-0.5 hundred million/g).Feed piglet with containing this probiotic feed additive, can improve weightening finish 20.5%, improve efficiency of feed utilization 9.5%, piglet daily gain contrast improves 19.5%.Preparation technology is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 10% bean sprouts juice, 0.4% glucose, 1% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds heavy 4 times calcium carbonate or wheat bran, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 4 hours, can obtain Bacillus cereus bacterium powder.
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 1.5% glucose, 1.5% peptone, the 1% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized; the thalline that centrifugation goes out; the thalline adding that accounts for protection liquid weight 40% is contained in the sodium glutamate and 15% greasiness milk powder frozen-dried protective liquid of weight 5%, can obtain Lactobacillus casei bacterium powder after the mixed freezing drying.
(3) preparation of VREF bacterium powder: contain weight 1% glucose, 0.5% peptone, 0.5% yeast extract, 0.1%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.1%KH
2PO
4Nutrient solution in, behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 40% to contain in 5% the sodium glutamate 10% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying.
(4) preparation of S. cervisiae bacterium powder: containing in weight 10% bean sprouts juice, 0.2% glucose, the 0.5% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 2 times of weights, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2 hours, can obtain S. cervisiae bacterium powder.
Embodiment 2 Bacillus cereus bacterium powder 10g, Lactobacillus casei bacterium powder 5g, VREF 5g, saccharomyces cerevisiae powder 2500g, lactose or glucose 10kg.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 200-500 hundred million/g.Add in the drinking water with 1: 1000 ratio, directly drink for 1-30 age in days fryer.Test group is than control group weightening finish 10.4% as a result, and the speed of growth improves 5.12%, and food consumption reduces by 4.87%.Preparation technology is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 8% bean sprouts juice, 0.2% glucose, 0.5% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 2 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2 hours, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 1% glucose, 0.5% peptone, the 0.5% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25% in the sodium glutamate that contains weight 3% and the 5% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 2% glucose, 1% peptone, 0.8% yeast extract, 0.3%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.3%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25% to contain in 3% sodium glutamate, the 5% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 5% bean sprouts juice, 0.35% glucose, the 1% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 2 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2 hours, can obtain S. cervisiae bacterium powder.
Embodiment 3 Bacillus cereus bacterium powder 14g, Lactobacillus casei bacterium powder 10g, VREF 10g, saccharomyces cerevisiae powder 3000g, corn flour or wheat bran powder 17kg.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 20-50 hundred million/g.Directly feed the meat calf as concentrated feed, improve 25.7% in increasing day by day through 40 days nursing calves, efficiency of feed utilization improves 23.1%, and the incidence of disease descends 21.7%.Preparation technology is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 5% bean sprouts juice, 0.35% glucose, 1.5% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 3 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 3 hours, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 2% glucose, 1% peptone, the 0.8% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 30% in the sodium glutamate that contains weight 4% and the 10% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 2% glucose, 1% peptone, 0.8% yeast extract, 0.3%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.3%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25-40% to contain in 4% sodium glutamate, the 10% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 8% bean sprouts juice, 0.35% glucose, the 1% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 3 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 3 hours, can obtain S. cervisiae bacterium powder.
Embodiment 4 Bacillus cereus bacterium powder 12g, Lactobacillus casei bacterium powder 15g, VREF 15g, saccharomyces cerevisiae powder 3000g, corn flour or wheat bran powder 20kg.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 200-500 hundred million/g.Feed rabbit with containing this probiotic feed additive, per diem beam 1%, 1.5%, 2% adds this preparation, and feed conversion rate improves 5%, 6.1%, 6.6% respectively, and M ﹠ M is starkly lower than control group.Vegetation technology is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 6% bean sprouts juice, 0.5% glucose, 1.5% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 3 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 3.5 hours, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in heavy 3% glucose, 1.5% peptone, the 1% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 40% in the sodium glutamate that contains weight 5% and the 14% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 3% glucose, 1.5% peptone, 1% yeast extract, 0.5%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.5%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 40% to contain in 5% sodium glutamate, the 15% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 10% bean sprouts juice, 0.5% glucose, the 1.5% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 4 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 4 hours, can obtain S. cervisiae bacterium powder.
Embodiment 5 Bacillus cereus bacterium powder 5g, Lactobacillus casei bacterium powder 5g, VREF 14g, saccharomyces cerevisiae powder 2100g, corn flour or wheat bran powder 15kg.By the probio additive that above prescription is made, every gram contains profitable probliotics and counts 200-500 hundred million/g.With containing on this probio 1% spray and the bait, be used for prawn and produce, test group increases kind of rate than control group and improves 16%, and feed conversion rate improves 21.4%.Survival rate improves 7.2%.Preparation technology is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 7% bean sprouts juice, 0.4% glucose, 0.8% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 2.5 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2.5 hours, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 2% glucose, 1.2% peptone, the 0.7% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 35% in the sodium glutamate that contains weight 3% and the 8% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 2% glucose, 0.8% peptone, 0.8% yeast extract, 0.4%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.4%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 30% to contain in 4% sodium glutamate, the 8% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 8% bean sprouts juice, 0.35% glucose, the 0.9% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 2.5 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 3 hours, can obtain S. cervisiae bacterium powder.
Claims (3)
1, a kind of feed addictive that is used to improve fowl poultry intestine immunity power is characterized in that: contain Bacillus cereus powder 5-15 part by weight ratio, Lactobacillus casei powder 5-15 part, VREF powder 5-15 part, S. cervisiae powder 2000-3000 part.
2, as the said feed addictive of claim 1, it is characterized in that: also be added with corn flour or wheat bran powder 10000-20000 part.
3, a kind of feed addictive production technology that is used to improve fowl poultry intestine immunity power, get Bacillus cereus powder 5-15 part, Lactobacillus casei powder 5-15 part, VREF powder 5-15 part, S. cervisiae powder 2000-3000 part by weight ratio, the bacterium powder preparation technique is as follows:
(1) preparation of Bacillus cereus bacterium powder: containing in weight 5-10% bean sprouts juice, 0.2-0.5% glucose, the 0.5-1.5% protein culture medium behind 32 ℃ of-36 ℃ of aerobic fermentations after Bacillus cereus bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the calcium carbonate or the wheat bran of 2-4 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2-4 hour, can obtain Bacillus cereus bacterium powder;
(2) preparation of Lactobacillus casei bacterium powder: containing in weight 1%-3% glucose, 0.5-1.5% peptone, the 0.5-1% yeast extract culture medium behind 32 ℃ of-36 ℃ of anaerobic fermentations after the lactobacillus casei bacterial strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25-40% in the sodium glutamate that contains weight 3-5% and the 5-15% greasiness milk powder frozen-dried protective liquid and can obtain Lactobacillus casei bacterium powder after the mixed freezing drying;
(3) preparation of VREF bacterium powder: contain 1%-3% glucose, 0.5-1.5% peptone, 0.5-1% yeast extract, 0.1-0.5%Na after the manure enterococcin strain seed liquor inserts and to have sterilized
2HPO
4, 0.1-0.5%KH
2PO
4Nutrient solution in behind 32 ℃ of-38 ℃ of anaerobic fermentations, the thalline that centrifugation goes out adds the thalline that accounts for protection liquid weight 25-40% in the sodium glutamate that contains 3-5%, the 5-15% greasiness milk powder frozen-dried protective liquid and can obtain VREF bacterium powder after the mixed freezing drying;
(4) preparation of S. cervisiae bacterium powder: containing in weight 5-10% bean sprouts juice, 0.2-0.5% glucose, the 0.5-1.5% peptone culture medium matrix behind 32 ℃ of-36 ℃ of aerobic fermentations after saccharomyces cerevisiae bacteria strain seed liquor inserts and to have sterilized, the thalline that centrifugation goes out, thalline adds the calcium carbonate or the wheat bran of 2-4 times of weight, be mixed with into wet granular, after under 40 ℃ of-55 ℃ of conditions dry 2-4 hour, can obtain S. cervisiae bacterium powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200610045954XA CN101028037A (en) | 2006-03-01 | 2006-03-01 | Feed additive for improving fowl and beast entric immune and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200610045954XA CN101028037A (en) | 2006-03-01 | 2006-03-01 | Feed additive for improving fowl and beast entric immune and its production |
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| Publication Number | Publication Date |
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| CN101028037A true CN101028037A (en) | 2007-09-05 |
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ID=38713721
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200610045954XA Pending CN101028037A (en) | 2006-03-01 | 2006-03-01 | Feed additive for improving fowl and beast entric immune and its production |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028104A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbiological feed and preparation method thereof |
| CN102028103A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbial bait additive and preparation method thereof |
| CN105192340A (en) * | 2014-11-20 | 2015-12-30 | 袁清珠 | Method for raising of high-added value beef cattle |
| CN111235074A (en) * | 2020-03-30 | 2020-06-05 | 广东锦玛绿色食品科技有限公司 | Composite functional microbial inoculum of saccharomyces cerevisiae |
| CN113317401A (en) * | 2021-05-25 | 2021-08-31 | 播恩集团股份有限公司 | Compound bioactive substance for replacing antibiotics and preparation method thereof |
| CN113841788A (en) * | 2021-09-26 | 2021-12-28 | 山东迅达康兽药有限公司 | Micro-ecological microcapsule coating preparation and preparation method thereof |
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2006
- 2006-03-01 CN CNA200610045954XA patent/CN101028037A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028104A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbiological feed and preparation method thereof |
| CN102028103A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbial bait additive and preparation method thereof |
| CN105192340A (en) * | 2014-11-20 | 2015-12-30 | 袁清珠 | Method for raising of high-added value beef cattle |
| CN111235074A (en) * | 2020-03-30 | 2020-06-05 | 广东锦玛绿色食品科技有限公司 | Composite functional microbial inoculum of saccharomyces cerevisiae |
| CN111235074B (en) * | 2020-03-30 | 2020-09-18 | 广东锦玛绿色食品科技有限公司 | Composite functional microbial inoculum of saccharomyces cerevisiae |
| CN113317401A (en) * | 2021-05-25 | 2021-08-31 | 播恩集团股份有限公司 | Compound bioactive substance for replacing antibiotics and preparation method thereof |
| CN113317401B (en) * | 2021-05-25 | 2021-11-30 | 播恩集团股份有限公司 | Compound bioactive substance for replacing antibiotics and preparation method thereof |
| CN113841788A (en) * | 2021-09-26 | 2021-12-28 | 山东迅达康兽药有限公司 | Micro-ecological microcapsule coating preparation and preparation method thereof |
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