CN102028104A - Microbiological feed and preparation method thereof - Google Patents
Microbiological feed and preparation method thereof Download PDFInfo
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- CN102028104A CN102028104A CN2010105908005A CN201010590800A CN102028104A CN 102028104 A CN102028104 A CN 102028104A CN 2010105908005 A CN2010105908005 A CN 2010105908005A CN 201010590800 A CN201010590800 A CN 201010590800A CN 102028104 A CN102028104 A CN 102028104A
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Abstract
The invention discloses microbiological feed which comprises the following components in parts by volume: 10-20 parts of bacillus cereous fermenting liquor, 20-40 parts of lactobacillus casei, 20-40 parts of enterococcus faecium liquor and 20-40 parts of saccharomyces cerevisiae liquor. The four bacteria are fixedly planted at the upper, the middle and the lower parts of the intestinal tract respectively and can inhibit the breeding and invasion of harmful bacteria in the whole intestinal tract, remove harmful substances at all parts of the intestinal tract, resist infection and maintain the flora balance of the digestive tract. All bacteria form joint flora which can grow under different conditions and have rapid and durable effect at different parts of the intestinal tract; a biological barrier is formed on the mucosa surface of the whole intestinal tract, prevents the growth and breeding of conditional pathogens of pathogens and removes endotoxin; and the multi-strain synergy can ensure that the microbiological feed is exerted to the optimal degree and is suitable for various host animals at maximum.
Description
Technical field
The present invention relates to a kind of microbiological feed, specifically, relate to a kind of fowl poultry intestinal microecology balance that is used to improve, strengthen livestock immunity and improve microbiological feed of efficiency of feed utilization and preparation method thereof.
Background technology
The research of existing probio also is only limited to the research to single bacterial classification, not at beneficial flora mutually promote, symbiosis, coordinate to promote to improve fowl poultry intestine immunity power, also do not coordinate to promote to improve the beneficial flora feed addictive and the technology of fowl poultry intestine immunity power.
Summary of the invention
The problem to be solved in the present invention is at above problem, and a kind of fowl poultry intestinal microecology balance that is used to improve is provided, and strengthens livestock immunity and improves microbiological feed of efficiency of feed utilization and preparation method thereof.
For overcoming the above problems, the present invention is by the following technical solutions: a kind of microbiological feed is characterized in that: described microbiological feed comprises the composition of following volume proportion: Bacillus cereus zymotic fluid 10-20 part, Lactobacillus casei 20-40 part, VREF bacterium liquid 20-40 part and S. cervisiae bacterium liquid 20-40 part.
As further improvement in the technical proposal:
Described microbiological feed comprises the composition of following volume proportion: 40 parts of 20 parts of Bacillus cereus zymotic fluids, 40 parts of Lactobacillus caseis, 20 parts of VREF bacterium liquid and S. cervisiae bacterium liquid.
A kind of preparation method of microbiological feed comprises the steps:
(1) preparation of Bacillus cereus bacterium liquid: Bacillus cereus bacterial strain seed liquor inserts in the culture medium after sterilization, culture medium contains the bean sprouts juice that weight ratio is 5-10%, the glucose of 0.2-0.5% and the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain Bacillus cereus bacterium liquid;
(2) preparation of Lactobacillus casei bacterium liquid: the lactobacillus casei bacterial strain seed liquor inserts in the culture medium after sterilization, culture medium contains the glucose that weight ratio is 1-3%, the peptone of 0.5-1.5% and the yeast extract of 0.5-1%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following anaerobic fermentations, obtain Lactobacillus casei bacterium liquid;
(3) preparation of VREF bacterium liquid: the manure enterococcin strain seed liquor inserts in the culture medium after sterilization, and culture medium contains the glucose that weight ratio is 1-3%, the peptone of 0.5-1.5%, the yeast extract of 0.5-1%, the Na of 0.1-0.5%
2HPO
4And 0.1-0.5%KH
2PO
4, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-38 ℃ of following anaerobic fermentations, obtains VREF bacterium liquid;
(4) preparation of S. cervisiae bacterium liquid: saccharomyces cerevisiae bacteria strain seed liquor inserts in the culture medium after sterilization, culture medium contains the bean sprouts juice that weight ratio is 5-10%, the glucose of 0.2-0.5% and the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain S. cervisiae bacterium liquid;
(5) Bacillus cereus bacterium liquid, Lactobacillus casei bacterium liquid, VREF bacterium liquid and S. cervisiae bacterium liquid are mixed according to above proportioning, obtain microbiological feed.
The present invention adopts above technical scheme, has the following advantages: 4 kinds of bacterium are colonizated in enteron aisle upper, middle and lower position respectively, can suppress breeding and the invasion of the harmful bacterium in the whole enteron aisle, remove each harmful substance of enteron aisle, and opposing is infected, and safeguards the archenteric flora balance.Each bacterial classification can both be grown under different condition one of enteron aisle different parts composition, acts on fast and lasting associating flora, forms one biological barrier on whole intestinal mucosa surface, prevents the growth and breeding of pathogenic bacteria conditioned pathogen, removes endotoxin.4 kinds of bacterium are united use, and the growth characteristic of each bacterial classification is different with function, have general character that specificity is also arranged, and the effect of many synergistic bacteriums can make it perform to the best, suitable to greatest extent all kinds of host animals.Four kinds of probios of the present invention are by the research of toxicology and pharmacology test, prove all safety and having no side effect of each bacterial strain.
The present invention is described in detail below in conjunction with embodiment.
The specific embodiment
Embodiment, a kind of microbiological feed comprises the composition of following volume proportion: 40 parts of 20 parts of Bacillus cereus zymotic fluids, 40 parts of Lactobacillus caseis, 20 parts of VREF bacterium liquid and S. cervisiae bacterium liquid, contain total viable count and reach 2-20 hundred million cfu/ml.
Above microbiological feed prepares in accordance with the following methods:
(1) preparation of Bacillus cereus bacterium liquid: Bacillus cereus bacterial strain seed liquor inserts in the culture medium after sterilization, it is 8% bean sprouts juice, 0.2% glucose and 0.5% peptone that culture medium contains weight ratio, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain Bacillus cereus bacterium liquid;
(2) preparation of Lactobacillus casei bacterium liquid: the lactobacillus casei bacterial strain seed liquor inserts in the culture medium after sterilization, it is 1% glucose, 0.5% peptone and 0.5% yeast extract that culture medium contains weight ratio, behind 32 ℃ of-36 ℃ of following anaerobic fermentations, surplus is the water as solvent, inoculum concentration is 0.3%, obtains Lactobacillus casei bacterium liquid;
(3) preparation of VREF bacterium liquid: the manure enterococcin strain seed liquor inserts in the culture medium after sterilization, and it is 2% glucose, 1% peptone, 0.8% yeast extract, 0.3% Na that culture medium contains weight ratio
2HPO
4And 0.3%KH
2PO
4, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-38 ℃ of following anaerobic fermentations, obtains VREF bacterium liquid;
(4) preparation of S. cervisiae bacterium liquid: saccharomyces cerevisiae bacteria strain seed liquor inserts in the culture medium after sterilization, it is 5% bean sprouts juice, 0.35% glucose and 1% peptone that culture medium contains weight ratio, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain S. cervisiae bacterium liquid;
(5) Bacillus cereus bacterium liquid, Lactobacillus casei bacterium liquid, VREF bacterium liquid and S. cervisiae bacterium liquid are mixed according to above proportioning, obtain microbiological feed.
Test 1 to probiotics strain involved in the present invention separately or unite use, is tested kind of a chicken intestinal flora, ight soil pH, harmful substance ammonia content and hydrogen sulfide content, water content and production performance.
Test is divided into 5 groups, and every group of 100 chickens are separately raised.A, B, C, D, be experimental group, the E group is control group.A group: in feed, add 0.3% Bacillus cereus bacterium liquid; B group: in feed, add 0.3% Lactobacillus plantarum and lactobacillus acidophilus bacterium liquid; C group: in feed, add 0.3% contain S. cervisiae bacterium liquid; D group: in feed, add 3% microbiological feed; E group: the conventional feed of feeding.When proceeding to the 30th day, test measures every index respectively, the 30th day kind chicken intestinal contents physical and chemical index situation (X ± S, n=6) as following table:
Grouping | A | B | C | D | E |
pH | 7.25±0.32 | 7.41±0.32 | 6.39±0.44 | 6.57±0.35 | 7.68±0.72 |
Moisture (%) | 75.49±3.54 | 76.45±4.21 | 77.44±3.95 | 77.29±3.39 | 77.64±3.28 |
Ammonia (mg/g) | 2.33±1.53 | 1.49±1.46 | 0.83±0.47 | 0.84±0.98 | 3.45±0.91 |
Hydrogen sulfide (mg/g) | 1.02±0.65 | 0.67±0.67 | 0.67±0.75 | 0.38±0.55 | 3.78±1.25 |
Lactic acid (mmol/g) | 0.21±0.10 | 0.22±0.18 | 0.31±0.03 | 0.48±0.04 | 0.09±0.04 |
Acetate (mmol/g) | 0.38±0.09 | 0.58±0.14 | 1.45±0.21 | 1.85±0.75 | 0.28±0.13 |
Butyric acid (mmol/g) | 0.17±0.12 | 0.06±0.03 | 0.14±0.05 | 0.09±0.04 | 0.05±0.02 |
Isobutyric acid (mmol/g) | 0.08±0.03 | 0.07±0.02 | 0.08±0.02 | 0.06±0.03 | 0.10±0.02 |
Test the 30th day kind chicken intestinal flora situation such as following table:
Grouping | Staphylococcus | Enterococcus | Escherichia coli | Lactobacillus | Bifidobacterium | Perfringens bacillus |
A | 7.04±0.29 | 5.60±0.40 | 6.23±0.21 | 8.07±0.38 | 8.76±0.21 | 5.04±0.54 |
B | 7.83±0.06 | 6.15±0.44 | 6.01±0.34 | 8.61±0.26 | 8.43±0.23 | 5.29±0.24 |
C | 7.37±0.67 | 6.03±0.28 | 5.33±0.45 | 8.33±0.42 | 8.72±0.23 | 4.69±0.55 |
D | 7.23±0.34 | 5.69±0.45 | 5.21±0.70 | 8.81±0.52 | 8.89±0.19 | 4.32±0.54 |
E | 8.52±0.30 | 7.84±0.31 | 6.91±0.18 | 7.07±0.76 | 7.80±0.13 | 7.35±0.68 |
Test and plant chicken production performance performance condition such as following table after the 30th day:
Index | A | B | C | D | E |
Survival rate (%) | 98 | 97 | 99 | 99 | 97 |
Laying rate (%) | 90.3 | 88.3 | 93.5 | 90.8 | 91.2 |
A day per unit area yield (g) | 49.5 | 50.3 | 51.4 | 52.3 | 50.2 |
Feedstuff-egg ratio | 1∶2.15 | 1∶2.15 | 1∶2.17 | 1∶2.15 | 1∶2.16 |
Rate of fertilization (%) | 96.9 | 97.8 | 97.1 | 96.4 | 96.8 |
Hatchability of fertile eggs (%) | 90.4 | 91.1 | 89.5 | 89.7 | 90.2 |
The result shows: the zoology test by 5 groups of different probiotics preparations that kind of chicken is fed is observed, profitable strain (Bifidobacterium, lactobacillus) and pernicious bacteria staphylococcus, enterococcus, enterobacteria, clostridium in Toxic in the chicken intestines (sulfide, ammonia) and organic acid (lactic acid, acetate, butyric acid, isobutyric acid) the chicken intestines), parameter such as intestinal contents moisture compares, the result shows that to adopt 4 kinds of bacterium mix preparations more effective than Lactobacillus casei and bacillus cereus two bacterium mix preparations and Lactobacillus casei or the single bacteria preparation of bacillus cereus.And to comparing the production performance aspect of laying hen, four bacterium mix preparations are better than two bacterium mix preparations and single bacteria preparation.Producing and the hatching aspect of performance does not have significant difference between 4 groups of bacteria preparations and the control group.
Test 2 is carried out the immune function of mice test to bacillus cereus, Lactobacillus casei and two kinds of bacterium mix preparations respectively.
Test is divided into 5 groups, every group of 10 BALB/c mouses, oral administration gavage, grouping administration.A, B, C, D, be experimental group, the E group is control group.Measured every index on the 15th day respectively in test.A group: the Bacillus cereus bacterium liquid that is equivalent to irritate stomach 1,000,000,000/kg; B group: the Lactobacillus casei bacterium liquid that is equivalent to irritate stomach 1,000,000,000/kg; C group: be equivalent to irritate Bacillus cereus bacterium liquid and the Lactobacillus casei bacterium liquid that stomach contains 500,000,000/kg respectively; D group: be equivalent to irritate Bacillus cereus bacterium liquid, Lactobacillus casei bacterium liquid, VREF bacterium liquid and the S. cervisiae liquid that stomach contains 2.5/kg respectively; E group: irritate stomach 10ml/kg physiological saline.Several probiotics preparations are to effect of immunologic function such as following table:
Index | A | B | C | D | E |
Have a liking for cytophagy rate (%) to huge | 26.65±0.87 | 27.23±0.56 | 31.42±0.89 | 36.12±0.88 | 22.13±0.64 |
Phagocytic index | 0,65±0.02 | 0.69±0.03 | 0.72±0.04 | 0.81±0.04 | 0.62±0.04 |
The mouse hemolysin is tired | 1∶200 | 1∶240 | 1∶260 | 1∶300 | 1∶120 |
NK cytoactive (%) | 19.32±7.67 | 18.53±5.67 | 20.24±4.78 | 28.344.12 | 15.31±6.23 |
Result of study shows that the immunoregulation effect index of the mix preparation of Lactobacillus casei and bacillus cereus is higher than the unitary agent of Lactobacillus casei and bacillus cereus respectively.
Implement 3, the microbiological feed of the embodiment of the invention and furazolidone and chloramphenicol are used for the curative effect contrast test of piglet white scour.
Viable count content is 1,500,000,000 CFU/ml in the microbiological feed, and furazolidone and chloramphenicol are bought by market.Microbiological feed gavages by 500,000,000/kg body weight, and the chloramphenicol consumption is 50mg/kg, and the furazolidone consumption is 10mg/kg.Test piglet system did not use the following routine piglet of other any drug therapies by the natural infection morbidity.
Efficacy determination: from medication, shrink effectively with sick pig anus in 3 days, relaxed state is normal, and it all normally be standard that ight soil is done solid moulding, spirit, appetite, as if there being the recidivist invalid.Different pharmaceutical is to the result of treatment such as the following table of piglet white scour:
Medicament categories | A number of taking medicine | Cure a number | Percentage % | Invalid number | Percentage % |
Probiotics preparation | 68 | 61 | 81.7 | 7 | 10.3 |
Chloramphenicol | 4 | 3 | 75 | 1 | 25 |
Furazolidone | 6 | 5 | 83.3 | 1 | 16.7 |
Microbiological feed is to result of treatment such as the following table of different days piglet:
The piglet age in days | Number | One day cure rate | Two days cure rates | Three days cure rates | Total cure rate |
3-10 | 33 | 6.1 | 3 | 84.7 | 93.9 |
11-20 | 11 | 27.3 | 36.4 | 36.6 | 100 |
21-30 | 10 | 20 | 10 | 50 | 80 |
More than 30 | 14 | 21.4 | 42.8 | 57.4 | 78.6 |
Add up to | 68 | 14.7 | 17.6 | 61 | 89.7 |
Implement 4, the microbiological feed of the embodiment of the invention is applied to the growth result test of piglet to middle pig.Viable count content is 1,500,000,000 CFU/ml in the microbiological feed, and A group and B group are experimental group, 15 piglets that the A group adopts same age in days, average starting weight 17.35kg; 15 piglets that the B group adopts same age in days, average starting weight 22.2kg; Control group adopts 15 piglets of same age in days, average starting weight 19.4kg; Experimental group and control group are all fed mixed feed, add microbiological feed in the experimental group, and addition is 0.1% of whole forage volumes.Average growing state such as following table:
Grouping | Average starting weight kg | Raise nose heave kg after 90s | Weightening finish kg | Rate of body weight gain % | Average feedstuff-meat ratio |
The A group | 17.35 | 48.4 | 31.05 | 29.4 | 4.36∶1 |
The B group | 22.2 | 51.07 | 28.87 | 16.56 | 4.58∶1 |
Control group | 19.4 | 44.6 | 25.2 | 5.35∶1 |
Test 5 is applied to the microbiological feed of the embodiment of the invention test of kind of laying rate of chicken.Viable count content is 1,500,000,000 CFU/ml in the microbiological feed, and test chicken is 39 age in days AA.PS kind chickens, 578 of test group 4 grid, 574 of control group 5 grid; Add microbiological feed in the test group, addition is 0.1% of whole forage volumes, feeds for 5 weeks continuously, the record egg number.Result such as following table:
Claims (3)
1. microbiological feed, it is characterized in that: described microbiological feed comprises the composition of following volume proportion: Bacillus cereus zymotic fluid 10-20 part, Lactobacillus casei 20-40 part, VREF bacterium liquid 20-40 part and S. cervisiae bacterium liquid 20-40 part.
2. a kind of microbiological feed as claimed in claim 1 is characterized in that: described microbiological feed comprises the composition of following volume proportion: 40 parts of 20 parts of Bacillus cereus zymotic fluids, 40 parts of Lactobacillus caseis, 20 parts of VREF bacterium liquid and S. cervisiae bacterium liquid.
3. a kind of preparation method of microbiological feed as claimed in claim 1 or 2, it is characterized in that: described preparation technology may further comprise the steps:
(1) preparation of Bacillus cereus bacterium liquid: Bacillus cereus bacterial strain seed liquor inserts in the culture medium after sterilization, culture medium contains the bean sprouts juice that weight ratio is 5-10%, the glucose of 0.2-0.5% and the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain Bacillus cereus bacterium liquid;
(2) preparation of Lactobacillus casei bacterium liquid: the lactobacillus casei bacterial strain seed liquor inserts in the culture medium after sterilization, culture medium contains the glucose that weight ratio is 1-3%, the peptone of 0.5-1.5% and the yeast extract of 0.5-1%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following anaerobic fermentations, obtain Lactobacillus casei bacterium liquid;
(3) preparation of VREF bacterium liquid: the manure enterococcin strain seed liquor inserts in the culture medium after sterilization, and culture medium contains the glucose that weight ratio is 1-3%, the peptone of 0.5-1.5%, the yeast extract of 0.5-1%, the Na of 0.1-0.5%
2HPO
4And 0.1-0.5%KH
2PO
4, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-38 ℃ of following anaerobic fermentations, obtains VREF bacterium liquid;
(4) preparation of S. cervisiae bacterium liquid: saccharomyces cerevisiae bacteria strain seed liquor inserts in the culture medium after sterilization, culture medium contains the bean sprouts juice that weight ratio is 5-10%, the glucose of 0.2-0.5% and the peptone of 0.5-1.5%, surplus is the water as solvent, inoculum concentration is 0.3%, behind 32 ℃ of-36 ℃ of following aerobic fermentations, obtain S. cervisiae bacterium liquid;
(5) Bacillus cereus bacterium liquid, Lactobacillus casei bacterium liquid, VREF bacterium liquid and S. cervisiae bacterium liquid are mixed according to above proportioning, obtain microbiological feed.
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CN102293349A (en) * | 2011-09-09 | 2011-12-28 | 合肥工业大学 | Composite probiotic meat chicken feed additive and application thereof |
CN103181479A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Microecological preparation special for newborn piglets and its preparation method |
CN106578351A (en) * | 2016-11-16 | 2017-04-26 | 山东众客食品有限公司 | Feed additive for increasing lean meat percentage as well as preparation method and application of feed additive |
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