CN102379361B - Antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation - Google Patents
Antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation Download PDFInfo
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Abstract
The invention belongs to the field of animal husbandry, in particular relates to an antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation. The method is characterized by comprising the following four aspects of: (1) adding a strain for feed into the fermented feed; (2) adding a bacterial suspension for drinking water into routine drinking water; (3) spraying a bacterial suspension for sterilizing in the feeding environment; and (4) planting greening trees and Chinese herbal medicines with sterilizing action to replace isolation strips between adjacent colony houses. The method provided by the invention obviously improves the feeding environment, improves the culture profit, lowers the feeding cost and culture risk, and provides a strong guarantee for obtaining high-quality antibiotic-free products.
Description
Technical field
The invention belongs to the livestock breeding field, be specifically related to the nonreactive cultivation method of a kind of feed, drinking-water, disinfecting bacterium and biological isolation associating.
Background technology
Along with the continuous expansion of breeding scale level and the raising of world agriculture economic globalization degree.China's livestock breeding faces unprecedented development space and huge challenge for existence.At present, there are many problems in China's livestock breeding, and for example: (1) human major product and requirement of by product to livestock breeding is more and more higher; (2) epidemic disease is more and more serious to the threat of the health of animal population and breeding environment; (3) the crop products price is constantly soaring, causes livestock breeding pressure increasing; (4) the international trade barrier affects the fast development of China's livestock breeding; (5) the breeding pollution problem is more and more outstanding, becomes the bottleneck of Animal husbandry development, and has caused the great attention of society and government; (6) abuse of antibiotic etc not only to animal, even causes larger hidden danger to health.
The existence of these problems has produced tremendous influence to livestock breeding, so Animal husbandry all begins actively to seek and a kind ofly can improve animal welfare, reduce environmental pollution, can improve again the cultural method of consumption safety.
At present, in the livestock breeding cultivation, add useful microorganism species and become a scientific and reasonable technology, but these bacterial classifications of how arranging in pairs or groups have become the project that each enterprise falls over each other to study.
The EM bacterium is that a kind of Mixed Microbes generally comprises Bacillus, saccharomycete, actinomycetes, azotobacter, cellulose decomposition bacteria, lactic acid bacteria etc., cultivates the effectively compound microbial flora that forms through specific process.Can be used for food and add, the farming disease harms control, waste water control etc.
Bacillus licheniformis cellular morphology and arrangement are shaft-like, Dan Sheng, capable of regulating flora (Bifidobacterium Bifidum, Bacillus acidi lactici, bacteroid, peptostreptococcus are had the promotion growth) imbalance reaches therapeutic purposes, can impel body to produce antibacterial substance, kill pathogenic bacteria (pathogenic bacteria such as staphylococcus, yeast-like fungus are had antagonism).Can produce the resistant activity material, and have unique biology and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria.
Bacillus subtilis is a kind of of bacillus.The subtilin that produces in the bacillus subtilis thalli growth process, polymixin, nystatin, gramicidins isoreactivity material, these active substances have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis consumes rapidly the free oxygen in the environment, causes the enteron aisle hypoxemia, promotes the growth of useful anaerobic bacteria, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacteria growth.Thalline self synthesizes the enzymes such as α-amylase, protease, lipase, cellulase, in digestive tract with animal body in digestive enzymes together play a role; The multiple B family vitamins such as energy synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.
Candida tropicalis is without O
2The time, decompose monose and produce CO
2And energy; O is arranged
2The time, monose thoroughly is decomposed into CO
2And H
2O.Candida tropicalis has very high nutritive value, particularly contains more protein, and a lot of B family vitamins, nucleic acid and mineral matter also can produce some health care active substances simultaneously.The candida tropicalis fermentation culture medium has the interferon of enhancing to tire, thereby the enhanced machine body immunity function is arranged.
Lactobacillus acidophilus belongs to lactobacillus, Gram-positive bacillus, and bar terminal rounded mainly exists in the small intestine, adjusts the gut flora balance, suppresses the propagation of enteron aisle undesirable microorganism.Lactobacillus acidophilus has antagonism to pathogenic microorganisms.Lactobacillus acidophilus also can produce lactic acid and acetic acid after fermenting in enteron aisle, can improve the availability of calcium, phosphorus, iron, promote the absorption of iron and vitamin D, produce vitamin K and Cobastab, can also reduce the absorption of cholesterol, and can reduce the injury of Radiation On Human body.
Bafillus natto is to have beneficial bacterium acidproof, heat-resistant quality; four hours survival rates are 100% under hydrochloric acid in gastric juice; have simultaneously powerful pathogen and suppress ability; to environment tolerance one of the bacterial classification of small intestine that preferably can go directly in the middle of the various beneficial bacterium; it is ecological to change the animal intestinal flora after oral; help digest function normalization, so that defecation is smooth and easy, keep the body physiological environmental protection.Can produce acid, the regulating intestinal canal flora, the enhancing animal cell immunity is put should.And can generate multiple protein enzyme (particularly alkali protease), carbohydrase, lipase, amylase, some complicated carbohydrate of degrading plant forage kind, thereby the conversion ratio of raising feed.
Streptococcus fecalis is again enterococcus faecalis.Use the C polysaccharide antigen, according to the Lan Shi serological classification, streptococcus can be divided into many groups.Streptococcus fecalis belongs to D group, and the D group streptococcus divides enterococcus and non-enterococcus two classes.The former comprises streptococcus fecalis, streptococcus faecalis and streptococcus durans, and the latter has bargen's streptococcus and streptococcus equinus.Streptococcus fecalis is additive for farm animal feed, can prevent and treat the cub fledgling poultry because of the diarrhea that intestinal bacilli illness causes, the effect that has the imbalance of regulating intestinal canal normal flora and promote to grow.
Bifidobacterium can be safeguarded enteron aisle normal bacteria colony balance, suppresses the growth of pathogen, prevents constipation, diarrhea and gastrointestinal disorders etc.Energy synthetic vitamin, amino acid and raising body reduce the Blood Cholesterol level to the absorption of calcium ion in enteron aisle, prevent and treat hypertension, and improve the anti-lactose of dairy products, improve digestibility.Safeguard enteron aisle normal bacteria colony balance, suppress the growth of pathogen, have the disorderly effect of the function of intestinal canal of adjustment; Synthetic vitamin, amino acid and raising body are to the absorption of calcium ion in enteron aisle; Strengthen the human immunologic function, prevent antibiotic side effect.
Summary of the invention
The nonreactive cultivation method that the purpose of this invention is to provide a kind of feed, drinking-water, disinfecting bacterium and biological isolation associating, can carry out each bacterial classification reasonably combined and then be applied in the links of cultivation, and take biological isolation so that whole cultivation is more scientific and reasonable, avoid the epidemic disease and the problem of environmental pollution that occur in the breeding process, improve the quality of livestock breeding major product and by product.
The nonreactive cultivation method of a kind of feed of the present invention, drinking-water, disinfecting bacterium and biological isolation associating comprises following four aspects:
(1) according to the standard that adds 2～4kg feed spawn in the feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the drinking water bacterial classification is made the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 80～160 quality, maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body, in the daily drinking water of biology, add the drinking water bacteria suspension, wherein, according to the parts by weight meter, the drinking water bacterial classification is by 2～3 parts of lactobacillus acidophiluss, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium are formulated;
(3) the disinfecting bacterium kind is made the disinfecting bacterium suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 50～100 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(4) plantation landscape tree and Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
Wherein, in aspect (2), hour can suitably improve drinking-water with the dilution ratio of bacteria suspension at institute's domesticated animal amount of drinking water, then will suitably turn down drinking-water when opposite amount of drinking water is larger with the dilution ratio of bacteria suspension.
Landscape tree in the aspect (4) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree, and Chinese herbal medicine is preferably honeysuckle.Isolation forms the transfer barrier that daily pathogen is spread as biology for the landscape tree of planting and Chinese herbal medicine, guarantee that after epidemic disease occurs infectious disease popular take air as main route of transmission even do not occur for few generation between letting animals feed, avoid as far as possible even stop cross-infection between the letting animals feed epidemic disease.
The present invention does not add any antibiotic in whole breeding process, reduce aquaculture cost, and avoided the healthy hidden danger of antibiotic to animal and human's body.
The invention has the advantages that: in breeding process, more than four aspect use in conjunction, add scientific and reasonable feeding and management, guaranteeing that letting animals feed obtains under the condition of suitable growth temperature, suppress growing of harmful bacteria in the feeding environment by drinking-water, feed and environment disinfected with the advantageous effect of microorganism, reduce ill probability.The air borne approach of the plantation pathogenic microorganism capable of blocking by landscape tree and Chinese herbal medicine is being avoided importing into and reducing and reduce the cultivation risk aspect the cross-infection of epidemic disease.The present invention has obviously improved feeding environment, has improved the cultivation income, has reduced feeding cost and cultivation risk, provides powerful guarantee for obtaining high-quality nonreactive product.
Embodiment
The present invention will be further described below in conjunction with example.
Embodiment 1:
Present embodiment comprises following 4 aspects take feeding of broiler as example:
(1) according to the standard that adds the 4kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 7,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 9,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 120 quality, beneficial bacterium valid density maintains the standard of 200,000,000 viable bacterias/kg in according to animal body, in biological drinking water, add the drinking water bacteria suspension after diluting, wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2 parts of lactobacillus acidophiluss, 2 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 2 parts of bafillus nattos, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums;
(3) the disinfecting bacterium kind is mixed with the disinfecting bacterium suspension that concentration is 8,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 60 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(4) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
The indices data that present embodiment obtains are as follows:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.62∶1 | 2.74Kg | 95％ | 0 | Nothing |
(2) the residual detection of medicine: (detecting index take the weight of animals as standard)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | Roxithromycin | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Embodiment 2:
Present embodiment is raised as example take the meat duck, comprises following 4 aspects:
(1) according to the standard that adds the 4kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 7,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 8,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 150 quality, beneficial bacterium valid density maintains the standard of 2.5 hundred million viable bacterias/kg in according to animal body, in biological drinking water, add the drinking water bacteria suspension after diluting, wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 2 parts of lactobacillus acidophiluss, 2 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 2 parts of bafillus nattos, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums;
(3) the disinfecting bacterium kind is mixed with the disinfecting bacterium suspension that concentration is 7,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to 1: 80 quality than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(4) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
The indices data that present embodiment obtains are as follows:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.82∶1 | 3.33Kg | 95％ | 0 | Nothing |
(2) the residual detection of medicine: (detecting index take the weight of animals as standard)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | Roxithromycin | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Claims (3)
1. a feed, drinking-water, disinfecting bacterium and biology are isolated the nonreactive cultivation method of uniting, and it is characterized in that comprising following four aspects:
(1) according to the standard that adds 2～4kg feed spawn in the feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;
(2) the drinking water bacterial classification is made the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to the quality of 1:80～160, maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body, in the daily drinking water of animal, add the drinking water bacteria suspension after diluting, wherein, according to the parts by weight meter, the drinking water bacterial classification is by 2～3 parts of lactobacillus acidophiluss, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium are formulated;
(3) the disinfecting bacterium kind is made the disinfecting bacterium suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the disinfecting bacterium suspension according to the quality of 1:50～100 than thin up, last spray disinfectant in feeding environment, wherein, the disinfecting bacterium kind adopts the EM bacterium;
(4) plantation landscape tree and Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
2. the nonreactive cultivation method of feed according to claim 1, drinking-water, disinfecting bacterium and biological isolation associating is characterized in that landscape tree in the aspect (4) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree.
3. the nonreactive cultivation method of feed according to claim 1, drinking-water, disinfecting bacterium and biological isolation associating is characterized in that the Chinese herbal medicine in the aspect (4) is preferably honeysuckle.
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| CN104178433B (en) * | 2014-09-04 | 2016-11-02 | 山东省农业科学院生物技术研究中心 | A kind of complex microorganism preparations of Urban River Water pollution control and preparation method thereof |
| CN107691348A (en) * | 2017-09-19 | 2018-02-16 | 郝天伟 | A kind of cultural method of low fat reed catkins chicken |
| CN108112541A (en) * | 2017-12-26 | 2018-06-05 | 杨凌欣益生态农业科技发展有限公司 | A kind of cultural method of livestock and poultry cultivation zero-emission |
| CN109601446B (en) * | 2018-11-23 | 2021-11-05 | 广东海纳川生物科技股份有限公司 | Preparation and application of special microbial preparation for stable water of prawn culture |
| CN109496981B (en) * | 2018-12-27 | 2021-08-27 | 北京市畜牧总站 | Method for improving egg laying quality of laying hens |
| CN110419643B (en) * | 2019-09-04 | 2023-05-23 | 刘泳君 | Fermented traditional Chinese medicine preparation and preparation method and application thereof |
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| CN102008016B (en) * | 2010-11-09 | 2013-06-19 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
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