CN102008016B - Probiotic solid powder and preparation method thereof - Google Patents
Probiotic solid powder and preparation method thereof Download PDFInfo
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- CN102008016B CN102008016B CN201010535819XA CN201010535819A CN102008016B CN 102008016 B CN102008016 B CN 102008016B CN 201010535819X A CN201010535819X A CN 201010535819XA CN 201010535819 A CN201010535819 A CN 201010535819A CN 102008016 B CN102008016 B CN 102008016B
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Abstract
The invention discloses probiotic solid powder and a preparation method thereof. The probiotic solid powder comprises a mixture of active probiotics and active zeolite, wherein the active probiotic content is between 12 and 50 billion colony-forming units (CFU)/gram; and the active zeolite mixture comprises the following components in percentage by weight: 30 to 50 percent of zeolite, 20 to 40 percent of attapulgite clay and 10 to 30 percent of bentonite. The probiotic solid powder provides a high-quality and low-cost antibiotic substitute product for livestock breeding; and the probiotic solid powder with 1 to 4 million CFU of live bacteria is added into each gram of feed, so the growth speed of chickens, pigs and fish can be improved by 2.0 to 15.6 percent, the conversion rate of the feed can be improved by 1.1 to 9.3 percent, and the diarrhea rate and the death rate of animals can be reduced obviously. The preparation method of the probiotic solid powder is simple, has low cost and can reduce environmental pollution; and the content of the obtained product bacteria is high.
Description
Technical field
The invention belongs to microorganism field, be specifically related to a kind of probiotic solid powder and preparation method thereof.
Background technology
In enteron aisle, microbial balance plays a role probio by improving, be can the Direct-fed animal useful living microorganism, probio is applied to have safety in feed, environmental protection, promotion growth of animal, suppress pathogen breeding, prevention disease of digestive tract, strengthen the animal immune function, improve efficiency of feed utilization, extra-nutrition, improve water environment, remove the effects such as toxin.
The probio of using in feed at present, is the solid powder product.The production technology of probio mainly contains two kinds of solid fermentation method and solution fermentations.The raw materials used processing of farm products accessory substance that mostly is of solid fermentation, the source is abundant, and is cheap, has the characteristics such as small investment, specification requirement are lower, but that the product of producing contains viable count is few, and purity is low.Liquid fermentation production probiotic products efficient is high, the bioactivator kind is many, viable count and purity high, but complex manufacturing, equipment investment is large, energy consumption is high, need make solid powder especially for the probio in feed, and being made powder product, probio in zymotic fluid mainly adopts vacuum freeze drying or spray drying technology, equipment requirement is high, has high input, and production cost is high, and can cause part viable bacteria inactivation in the high temperature drying process, affect the living bacteria count amount in product.The present invention aims to provide a kind of new method that is produced the solid powder product by the liquid probiotic bacterium, to solve the problems referred to above that exist in traditional handicraft.
Summary of the invention
The object of the present invention is to provide a kind of probiotic solid powder.
Another object of the present invention is to provide a kind of preparation method of probiotic solid powder.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of probiotic solid powder, it is that this probiotic solid powder contains profitable probliotics and activated zeolite mixture, and in described activated zeolite mixture, the weight percentage of each component is: zeolite 30%～50%, attapulgite clay 20%～40%, bentonite 10%～30%.
Above-mentioned probiotic solid powder, its be described in this probiotic solid powder the content of active probiotic be 120～50,000,000,000 CFU/g (CFU is CFU).
Above-mentioned probiotic solid powder, it is that described probio is bacillus.Described bacillus is bacillus subtilis (Bacillus subtilis) and/or bacillus licheniformis (Bacillus licheniformis).
A kind of preparation method of probiotic solid powder, it is that the method comprises the following steps: by weight the percentage meter with probiotics fermention liquid 60%～95% and activated zeolite mixture 5%～40% mix and blend after, input heat in absorber load, standing, isolated by filtration, the solid material that separation is obtained carries out drying under 45～70 ℃, dried solid material is crushed to grain fineness≤0.1 millimeter, namely makes probiotic solid powder.
The preparation method of above-mentioned probiotic solid powder, it is that speed of agitator is 100～200 rev/mins, and when heating load, temperature is 20～50 ℃, and adsorption time is 5～60 minutes.
The preparation method of above-mentioned probiotic solid powder, its weight percentage that is described each component of activated zeolite mixture is: zeolite 30%～50%, attapulgite clay 20%～40%, bentonite 10%～30%.
The preparation method of above-mentioned probiotic solid powder, it is that the content of active probiotic in described probiotics fermention liquid is 50～10,000,000,000 CFU/g.
The preparation method of above-mentioned probiotic solid powder, it is that described probio is bacillus.Described bacillus is bacillus subtilis (Bacillus subtilis) and/or bacillus licheniformis (Bacillus licheniformis).
The detailed description of content of the present invention:
The production method of probiotic solid powder is fermentation, absorption, separation, drying, pulverizes, is packaged as finished product.
Probiotic solid powder is comprised of probio, zeolite, attapulgite clay, bentonite.
Probio is a kind of additive for microbe feedstuff of work, and in enteron aisle, the balance of microorganism plays a role by improving.
Zeolite is the general name that is the porous hydrous alumino silicates crystal of frame shape structure, is interconnected to form frame shape sial oxygen skeleton by sial oxygen tetrahedron, and forms therein many ducts and hole, and specific area is large, has selective absorption and shape selective catalysis characteristic; Zeolite surface also has very large dispersion force and electrostatic force, therefore its absorption affinity is larger; Zeolite also has larger cation exchange capacity, catalysis, heat endurance, and bacteriotoxin and mycotoxin in adsorbable intestines and stomach.Studies show that, zeolite is applied in feed and aquaculture, without any side effects to animal, can promote growth of animal, absorption poisonous and harmful substance, strengthen animal immunizing power, prevent disease, raising animal product quality and fanning economics, and have the effect that improves environment.
Attapulgite clay has unique performances such as absorption, decolouring, suspension, thixotroping, colloid, filling, rheological characteristic, heat endurance, stickiness and salt-resistance, has larger interlamellar spacing or aperture.
Volumetric expansion after the bentonite suction, volume energy are expanded and are increased several times to tens times, and bentonite can disperse to be soliquid in aqueous medium, and this suspension has certain viscosity and thixotropy.
After the common modification of zeolite, attapulgite clay and bentonite, not only can adsorb in a large number with parasitic probiotics fermention liquid in probio, also help the drying of carrying out probiotic solid powder and process.
The agar Main Function is that fluid nutrient medium is solidified.
Peptone by protein through enzyme, acid, basic hydrolysis and the water soluble mixt that a kind of peptone, peptide, the amino acid that obtain form be mainly microorganism nitrogenous source and vitamin be provided.
Glucose provides carbon source and the energy for microorganism.
Beef extract provides carbon source, the energy, phosphate and vitamin for microorganism.
Yeast extract is rich in protein, amino acids, peptide class, nucleotides, B family vitamin, trace element, and Main Function is to replenish nitrogenous source, provides growth of microorganism required various vitamins, amino acid and growth factor.
Sodium chloride provides nutrient sodium and chlorine, and keeps osmotic pressure.
Dipotassium hydrogen phosphate is the source of phosphorus, potassium, and the while is as the pH value of buffer system maintain base.
Magnesium sulfate reduces the pace of change of pH value as buffer, reach the effect of stablizing acid-base value.Magnesium is indispensable factor when in the livestock and poultry body, bone process and contraction of muscle are made in participation, and the metabolism in the livestock and poultry body and nervous function are played an important role.
The present invention is achieved by following technical proposals:
1, the production method of probiotic solid powder: after the batching of probiotic solid powder is carried out mix and blend, input the load of heating in absorber, speed of agitator is controlled at 100～200 rev/mins, temperature is controlled at 20～50 ℃, adsorption time is controlled at 5～60 minutes, separate with filter-cloth filtering after standing 5～20 minutes, separate the solid material that obtains and carry out drying under 45～70 ℃, dried solid material is pulverized, granular material grain fineness after pulverizing≤0.1 millimeter is packaged as the probiotic solid powder finished product with granular material;
The batching of described probiotic solid powder is comprised of following component by weight percentage: probiotics fermention liquid 60～95%, activated zeolite mixture 5～40%.
2, the production method of described probiotics fermention liquid:
(1) under aseptic condition, the probio bacterial classification of preservation is placed in selection isolation medium in bacteriological incubator, and the bacteriological incubator temperature is controlled at 36～38 ℃, constant temperature culture 14～18 hours, put into refrigerator after cooling and do to produce the bacterial classification preservation, refrigerator temperature is controlled at 2～4 ℃;
Select the batching of isolation medium to be formed by following component by weight percentage: agar 1～3%, peptone 0.5～1.5%, glucose 0.2～0.8%, beef extract 0.2～0.8%, yeast extract 0.2～0.8%, sodium chloride 0.2～0.8% and water 93～96%;
The batching of producing bacterial classification is comprised of following component by weight percentage: probio bacterial classification 1～10% and selection isolation medium 90～99%.
(2) under aseptic condition, to produce strain transfer to the eggplant bottle inclined-plane that the culture medium that spreads cultivation is arranged, put into bacteriological incubator, the bacteriological incubator temperature is controlled at 36～38 ℃, constant temperature culture 10～14 hours, to the inclined-plane cover with thicker, opaque and the lawn of obvious wrinkle is arranged after be the bacterial classification after spreading cultivation;
The batching of culture medium of spreading cultivation is comprised of following component by weight percentage: murphy juice 16～22%, bean sprout juice 8～12%, glucose 1～3%, agar 1.8～2.2%, peptone 0.5～1.5%, dipotassium hydrogen phosphate 0.05～0.15%, magnesium sulfate 0.02～0.08% and water 60～70%;
The batching of bacterial classification of spreading cultivation is comprised of following component by weight percentage: produce bacterial classification 1～10% and the culture medium 90～99% that spreads cultivation.
(3) bacterial classification after spreading cultivation adds in the liquid fermentation tank that contains basal medium and ferments, and fermentation time is controlled at 16～32 hours, and temperature is controlled at 36～38 ℃, and the liquid after fermentation is probiotics fermention liquid;
The batching of basal medium is comprised of following component by weight percentage: glucose 1～3%, peptone 0.5～1.5%, sodium chloride 0.2～0.8%, dipotassium hydrogen phosphate 0.05～0.15%, magnesium sulfate 0.02～0.08% and water 94～98%;
The batching of probiotics fermention is comprised of following component by weight percentage: the bacterial classification 1～10% after spreading cultivation and basal medium 90～99%;
In the probiotics fermention process, the biomass in zymotic fluid and zymotic fluid are directly proportional at 660nm place light absorption value, and light absorption value rises, and biomass increases, and proceeds fermentation, and light absorption value descends and shows that biomass no longer increases, and stops fermentation.
3, the production method of described activated zeolite mixture: (1) carries out fragmentation after the batching of zeolite mixture is mixed, the grain fineness after fragmentation≤2 millimeter; (2) will carry out roasting in the input of the zeolite mixture after fragmentation roaster, sintering temperature is controlled to be 280～400 ℃; (3) will the zeolite mixture after roasting carry out abrasive dust in the input flour mill, the grain fineness after abrasive dust≤0.074 millimeter is packaged as the activated zeolite mixture standby;
The batching of zeolite mixture is comprised of following component by weight percentage: zeolite 30～50%, attapulgite clay 20～40% and bentonite 10～30%.
Beneficial effect of the present invention:
Probiotic solid powder of the present invention, viable count can reach 120～50,000,000,000 CFU/g, and the number of live bacteria of probiotics of conventional solid fermentation method and solution fermentation production only has 50～10,000,000,000 CFU/g.
The preparation method of probiotic solid powder of the present invention, can simplify process for separating and purifying, improve probiotic products viable count and quality, reduce production costs, environmental contamination reduction, for aquaculture provides high-quality, inexpensive antibiotic substitute products.
Probiotic solid powder is made an addition in feed, the probio that animal is taken in enteron aisle by with harmful bacteria competition oxygen, nutrition or field planting site, suppress harmful bacteria propagation, thereby improve the balance of intestinal flora, animal is had promote grow, improve food conversion ratio, the effects such as anti-oxidant, immunostimulation, improvement health, prevent disease, treatment disease, raising product quality and fanning economics, and have the effect that improves water environment.
Evidence: the probiotic solid powder that adds 100～4,000,000 CFU viable bacterias in every gram feed can improve the speed of growth 2.0～15.6% of broiler chicken, pig and fish, improve food conversion ratio 1.1～9.3%, and can obviously reduce diarrhea rate and the death rate of animal.
The specific embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1 preparation zeolite mixture
(1) with the batching of zeolite mixture by following percentage by weight: carry out fragmentation after zeolite 40%, attapulgite clay 35% and bentonite 25% mix, the grain fineness after fragmentation≤2 millimeter; (2) will carry out roasting in the input of the zeolite mixture after fragmentation roaster, sintering temperature is controlled to be 300～320 ℃; (3) will the zeolite mixture after roasting carry out abrasive dust in the input flour mill, the grain fineness after abrasive dust≤0.074 millimeter is packaged as the activated zeolite mixture standby.
Embodiment 2 preparation fermentation of bacillus liquid
2.1 preparation fermentation of bacillus subtilis liquid
(1) under aseptic condition, the Bacillus subtilis strain of preservation is placed in selection isolation medium in bacteriological incubator, and the bacteriological incubator temperature is controlled at 37～38 ℃, constant temperature culture 15～16 hours, put into refrigerator after cooling and do to produce the bacterial classification preservation, refrigerator temperature is controlled at 3～4 ℃;
Select the batching of isolation medium to be formed by following component by weight percentage: agar 2.4%, peptone 1.3%, glucose 0.6%, beef extract 0.4%, yeast extract 0.4%, sodium chloride 0.3% and water 95%;
The batching of producing bacterial classification is comprised of following component by weight percentage: Bacillus subtilis strain 6% and selection isolation medium 94%.
(2) under aseptic condition, to produce strain transfer to the eggplant bottle inclined-plane that the culture medium that spreads cultivation is arranged, put into bacteriological incubator, the bacteriological incubator temperature is controlled at 37～38 ℃, constant temperature culture 12～13 hours, to the inclined-plane cover with thicker, opaque and the lawn of obvious wrinkle is arranged after be the bacterial classification after spreading cultivation;
The batching of culture medium of spreading cultivation is comprised of following component by weight percentage: murphy juice 18%, bean sprout juice 10%, glucose 2%, agar 1.8%, peptone 1.1%, dipotassium hydrogen phosphate 0.08%, magnesium sulfate 0.02% and water 67%;
The batching of bacterial classification of spreading cultivation is comprised of following component by weight percentage: produce bacterial classification 7% and the culture medium 93% that spreads cultivation.
(3) bacterial classification after spreading cultivation adds in the liquid fermentation tank that contains basal medium and ferments, and fermentation time is controlled at 26～28 hours, and temperature is controlled at 37～38 ℃, and the liquid after fermentation is fermentation of bacillus subtilis liquid;
The batching of basal medium is comprised of following component by weight percentage: glucose 2.4%, peptone 1.3%, sodium chloride 0.2%, dipotassium hydrogen phosphate 0.06%, magnesium sulfate 0.04% and water 96%;
The batching of fermentation of bacillus subtilis is comprised of following component by weight percentage: the bacterial classification 8% after spreading cultivation and basal medium 92%;
In the fermentation of bacillus subtilis process, the biomass in zymotic fluid and zymotic fluid are directly proportional at 660nm place light absorption value, and light absorption value rises, and biomass increases, and proceeds fermentation, and light absorption value descends and shows that biomass no longer increases, and stops fermentation.
Record that in the zymotic fluid of bacillus subtilis, viable count content is 8,600,000,000 CFU/g.
2.2 preparation the lichen bacillus ferments liquid
(1) under aseptic condition, the Bacillus licheniformis strain of preservation is placed in selection isolation medium in bacteriological incubator, and the bacteriological incubator temperature is controlled at 37～38 ℃, constant temperature culture 15～16 hours, put into refrigerator after cooling and do to produce the bacterial classification preservation, refrigerator temperature is controlled at 3～4 ℃;
Select the batching of isolation medium to be formed by following component by weight percentage: agar 2.4%, peptone 1.3%, glucose 0.6%, beef extract 0.4%, yeast extract 0.4%, sodium chloride 0.3% and water 95%;
The batching of producing bacterial classification is comprised of following component by weight percentage: Bacillus licheniformis strain 6% and selection isolation medium 94%.
(2) under aseptic condition, to produce strain transfer to the eggplant bottle inclined-plane that the culture medium that spreads cultivation is arranged, put into bacteriological incubator, the bacteriological incubator temperature is controlled at 37～38 ℃, constant temperature culture 12～13 hours, to the inclined-plane cover with thicker, opaque and the lawn of obvious wrinkle is arranged after be the bacterial classification after spreading cultivation;
The batching of culture medium of spreading cultivation is comprised of following component by weight percentage: murphy juice 18%, bean sprout juice 10%, glucose 2%, agar 1.8%, peptone 1.1%, dipotassium hydrogen phosphate 0.08%, magnesium sulfate 0.02% and water 67%;
The batching of bacterial classification of spreading cultivation is comprised of following component by weight percentage: produce bacterial classification 7% and the culture medium 93% that spreads cultivation.
(3) bacterial classification after spreading cultivation adds in the liquid fermentation tank that contains basal medium and ferments, and fermentation time is controlled at 26～28 hours, and temperature is controlled at 37～38 ℃, and the liquid after fermentation is the lichen bacillus ferments liquid;
The batching of basal medium is comprised of following component by weight percentage: glucose 2.4%, peptone 1.3%, sodium chloride 0.2%, dipotassium hydrogen phosphate 0.06%, magnesium sulfate 0.04% and water 96%;
The batching of the lichen bacillus ferments is comprised of following component by weight percentage: the bacterial classification 8% after spreading cultivation and basal medium 92%;
In the lichen bacillus ferments process, the biomass in zymotic fluid and zymotic fluid are directly proportional at 660nm place light absorption value, and light absorption value rises, and biomass increases, and proceeds fermentation, and light absorption value descends and shows that biomass no longer increases, and stops fermentation.
Record that in the zymotic fluid of bacillus licheniformis, viable count content is 9,000,000,000 CFU/g.
The preparation of embodiment 3 bacillus solid powders and bacterial content are measured process
3.1 the preparation process of bacillus subtilis solid powder
the batching of bacillus subtilis solid powder is comprised of following component by weight percentage: fermentation of bacillus subtilis liquid 70%, activated zeolite mixture 30%, after above batching is carried out mix and blend, input the load of heating in absorber, speed of agitator is controlled at 150～180 rev/mins, temperature is controlled at 35～45 ℃, adsorption time is controlled at 45～50 minutes, separate with filter-cloth filtering after standing 15 minutes, separate the solid material that obtains and carry out drying under 55～65 ℃, dried solid material is pulverized, granular material grain fineness after pulverizing≤0.1 millimeter, granular material is packaged as bacillus subtilis solid powder finished product.
3.2 the preparation process of bacillus licheniformis solid powder
the batching of bacillus licheniformis solid powder is comprised of following component by weight percentage: the lichen bacillus ferments liquid 75%, activated zeolite mixture 25%, after above batching is carried out mix and blend, input the load of heating in absorber, speed of agitator is controlled at 150～180 rev/mins, temperature is controlled at 35～45 ℃, adsorption time is controlled at 45～50 minutes, separate with filter-cloth filtering after standing 15 minutes, separate the solid material that obtains and carry out drying under 55～65 ℃, dried solid material is pulverized, granular material grain fineness after pulverizing≤0.1 millimeter, granular material is packaged as bacillus licheniformis solid powder finished product.
3.3 the preparation process of bacillus subtilis and bacillus licheniformis hybrid solid pulvis
the batching of hybrid solid pulvis is comprised of following component by weight percentage: fermentation of bacillus subtilis liquid 40%, fermentation of bacillus subtilis liquid 40%, activated zeolite mixture 20%, after above batching is carried out mix and blend, input the load of heating in absorber, speed of agitator is controlled at 150～180 rev/mins, temperature is controlled at 35～45 ℃, adsorption time is controlled at 45～50 minutes, separate with filter-cloth filtering after standing 15 minutes, separate the solid material that obtains and carry out drying under 55～65 ℃, dried solid material is pulverized, granular material grain fineness after pulverizing≤0.1 millimeter, granular material is packaged as bacillus subtilis and bacillus licheniformis hybrid solid pulvis finished product.
Detect bacterial content in the bacillus solid powder according to the assay method of bacterium number in solid powder:
1 reagent and culture medium
1.1 SPSS:
Sodium chloride 8.5g, distilled water is settled to 1000mL, 121 ℃ of autoclaving 15min.
1.20.1mol/L NaOH solution:
NaOH 4g, distilled water is settled to 1000mL, 121 ℃ of autoclaving 15min.
1.3 agar medium:
Glucose 5g, beef extract 5g, yeast extract 5g, peptone 10g, NaC l5g, agar 20g transfers to 7.0-7.2 with 0.1mol/L NaOH solution with the pH value, and distilled water is settled to 1000mL, 121 ℃ of autoclaving 15min.
2 operating procedures
2.1 the dilution of sample
Take the 1g sample, put into the aseptic centrifuge tube (bottle presets 1-2 sterile glass beads) that fills 9mL physiological saline, oscillator homogeneous 1min-2min makes the even liquid of sample of 1: 10.
Draw 1: 10 even liquid 1mL of sample with micropipettor, slowly inject the aseptic centrifuge tube that fills 9mL physiological saline along tube wall, oscillator homogeneous 1min-2min makes the even liquid of sample of 1: 100.
Draw 1: 100 even liquid 0.1mL of sample with micropipettor, slowly inject the aseptic centrifuge tube that fills 0.9mL physiological saline along tube wall, and piping and druming repeatedly, it is mixed, make the even liquid of sample of 1: 1000.
Press aforesaid operations, successively the even liquid of tenfold ascending series dilute sample processed.
Often increase progressively dilution 1 time, must use micropipettor rifle head instead.
2.2 plate count
Culture medium with 15mL-20mL is poured in each plate in advance, after to be cooled solidifying, chooses 10
-8, 10
-9And 10
-10Three even liquid of dilution sample are drawn 100 μ l with micropipettor respectively and are inoculated on culture medium, carry out coated plate with L-type or triangle glass bar.37 ℃ of lower constant temperature aerobics are cultivated 24h, and choose the clump count scope and count at the culture dish of 30-300, the conversion extension rate, result of calculation represents (doing simultaneously three parallel repetitions, results averaged) with hundred million/g.
3 experimental results: the bacterium number that records in bacillus subtilis solid powder, bacillus licheniformis solid powder, bacillus subtilis and bacillus licheniformis hybrid solid pulvis is respectively 26,000,000,000 CFU/g, 35,000,000,000 CFU/g, 38,000,000,000 CFU/g.
Application and the effect of embodiment 4 probiotic solid powders in animal feeding
Evidence: add the probiotic solid powder for preparing in the embodiment 3 of 100～4,000,000 CFU viable bacterias and can improve the speed of growth 2.0～15.6% of broiler chicken, pig and fish in every gram feed, improve food conversion ratio 1.1～9.3%, and can obviously reduce diarrhea rate and the death rate of animal.
Claims (9)
1. probiotic solid powder, it is characterized in that this probiotic solid powder contains active probiotic and activated zeolite mixture, in described activated zeolite mixture, the weight percentage of each component is: zeolite 30%～50%, attapulgite clay 20%～40%, bentonite 10%～30%; The preparation method of described probiotic solid powder comprises the following steps: by weight the percentage meter with probiotics fermention liquid 60%～95% and activated zeolite mixture 5%～40% mix and blend after, input heat in absorber load, standing, isolated by filtration, the solid material that separation is obtained carries out drying under 45～70 ℃, dried solid material is crushed to grain fineness≤0.1 millimeter, namely makes probiotic solid powder.
2. probiotic solid powder according to claim 1, the content that it is characterized in that active probiotic in described probiotic solid powder is 120～50,000,000,000 CFU/g.
3. probiotic solid powder according to claim 1, is characterized in that described active probiotic is bacillus.
4. probiotic solid powder according to claim 3, is characterized in that described bacillus is bacillus subtilis and/or bacillus licheniformis.
5. the preparation method of a probiotic solid powder, it is characterized in that the method comprises the following steps: by weight the percentage meter with probiotics fermention liquid 60%～95% and activated zeolite mixture 5%～40% mix and blend after, input heat in absorber load, standing, isolated by filtration, the solid material that separation is obtained carries out drying under 45～70 ℃, dried solid material is crushed to grain fineness≤0.1 millimeter, namely makes probiotic solid powder; The weight percentage of described each component of activated zeolite mixture is: zeolite 30%～50%, attapulgite clay 20%～40%, bentonite 10%～30%.
6. the preparation method of probiotic solid powder according to claim 5, is characterized in that speed of agitator is 100～200 rev/mins, and when heating load, temperature is 20～50 ℃, and load time is 5～60 minutes.
7. the preparation method of probiotic solid powder according to claim 5, the content that it is characterized in that active probiotic in described probiotics fermention liquid is 50～10,000,000,000 CFU/g.
8. the preparation method of probiotic solid powder according to claim 5, is characterized in that described probio is bacillus.
9. the preparation method of probiotic solid powder according to claim 8, is characterized in that described bacillus is bacillus subtilis and/or bacillus licheniformis.
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| CN102334610A (en) * | 2011-09-05 | 2012-02-01 | 李文有 | Antibiotic-free environment-friendly biological feed and production method thereof |
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| CN102379368B (en) * | 2011-09-29 | 2013-03-20 | 滨州市正元畜牧发展有限公司 | Non-resistance cultivation method with joint application of feed strain, disinfectant bacterium and biological isolation |
| CN102379361B (en) * | 2011-09-29 | 2013-03-20 | 滨州市正元畜牧发展有限公司 | Antibiotic-free culture method of combined application of bacteria for feed, drinking water and sterilizing and biological isolation |
| CN102379373B (en) * | 2011-09-29 | 2013-05-08 | 滨州市正元畜牧发展有限公司 | Non-resistance cultivation method with joint application of feed strain, drinking water strain and biological isolation |
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| CN1596693A (en) * | 2004-08-31 | 2005-03-23 | 南开大学 | Beneficial bacteria preparation for poultry and its preparation method |
| CN101856063A (en) * | 2009-04-02 | 2010-10-13 | 大连泰恒生物科技有限公司 | Novel feed for sea cucumbers |
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| CN102008016A (en) | 2011-04-13 |
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