CN1596693A - Beneficial bacteria preparation for poultry and its preparation method - Google Patents
Beneficial bacteria preparation for poultry and its preparation method Download PDFInfo
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- CN1596693A CN1596693A CNA2004100203873A CN200410020387A CN1596693A CN 1596693 A CN1596693 A CN 1596693A CN A2004100203873 A CNA2004100203873 A CN A2004100203873A CN 200410020387 A CN200410020387 A CN 200410020387A CN 1596693 A CN1596693 A CN 1596693A
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- bacillus
- actinomyces
- lactic acid
- acid bacteria
- saccharomycete
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Abstract
A useful bacteria feed for fowls is prepared from bacillus, actinomycess, lactobacillus and deactivated yeast through proportional mixing, stage-one enlarge culture in liquid, stage-two enlarge culture in liquid immobilizing them on the surface of wheat bran, drying, and pulverizing.
Description
Technical field
The present invention relates to a stud bird and use probiotics bacterial preparation and preparation method thereof.
Background technology
The probiotics bacterial preparation is to support feed additive for fowls, and it contains multiple probio, can regulate the ecological balance of animal intestinal microorganism species, promotes the dominant growth of beneficial bacterium, has the effect of prevention eqpidemic disease and promotion growth.Pertinent literature shows by analysis, both at home and abroad bibliographical informations of seeing feed probiotic and feed addictive more, and they are made up of different floras, are applicable to the breed of animals such as bird, domestic animals, fish.As being the probiotic composition of Main Ingredients and Appearance with Bacillus acidi lactici KE01; With bacillus CIP5832 or enzyme is the feed addictive of Main Ingredients and Appearance; (the bacterium number is 10 with lactic acid bacteria, bacillus
6), actinomyces, filamentous fungi or enzyme be the tasteless feed of Main Ingredients and Appearance; With the probiotic 3000 that multiple beneficial bacterium such as Miyarisan, lactic acid bacteria, Bifidobacterium, bacillus, saccharomycete are formed, viable count content 〉=2,500,000,000 CFU/g; Main Ingredients and Appearance comprises: saccharomycete, bacillus licheniformis, bacillus cereus, Bifidobacterium, digestive ferment produce bacterium, extraordinary amino acid produces extraordinary nutriment such as bacterium, vitamin and ubiquinone and produces bacterium, commodity are by name: ecological precious EM838, viable count reaches more than 20,000,000,000 in every gram pulvis.But its density and activity are still waiting to improve.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of deodorizing benefit fowl living, high density and activity to use probiotics bacterial preparation and preparation method thereof.
The present invention solves this technical problem the technical scheme that is adopted:
The mixed bacterial that little ecological probiotics bacterial preparation mainly is made up of bacillus, actinomyces, lactic acid bacteria, add the saccharomycete of deactivation, wherein the percentage by weight of all kinds of bacterium is: bacillus 50～70%, actinomyces 10～30%, lactic acid bacteria 20～40%, inactive yeast bacterium 5～10%.
The preparation method of little ecological probiotics bacterial preparation comprises the steps:
1) each slant strains of activation preservation is shaken bottle respectively and is cultivated; Saccharomycete, bacillus and actinomyces are used the soluble starch medium culture, pH7.0～7.5,100～140 ℃ autoclaving 20-30 minute; Lactic acid bacteria is directly cultivated with the sterilization fresh cow milk; Bacillus was cultivated 20～24 hours for 30～37 ℃; Actinomyces and lactic acid bacteria were cultivated 48～50 hours for 28～30 ℃;
2) saccharomycete, bacillus, the level liquid of actinomyces and lactic acid bacteria enlarge cultivates (20L jar); Inoculum concentration 10%～20%;
3) saccharomycete, bacillus, the secondary fluid enlargement culture of actinomyces and lactic acid bacteria (200L jar); Inoculum concentration 10%～20%;
4) with wheat bran be carrier respectively solid enlarge to cultivate and be immobilized onto on the wheat bran;
5) with saccharomycete in 75 ℃ of left and right sides deactivations 45 minutes; With bacillus, actinomyces and lactic acid bacteria dry below 30～40 ℃, pulverize;
6) bacillus, actinomyces and lactic acid bacteria are mixed by following proportioning: bacillus 50～70%, actinomyces 10～30%, lactic acid bacteria 20～40%.The saccharomycete that adds simultaneously 5～10% deactivation again.
Beneficial effect of the present invention
1) probio is immobilized onto on the wheat bran, makes the living bacteria count (CFU) of every gram bacteria preparation almost reach most probable number MPN, yet there are no the report of high bacteria containing amount bacteria preparation like this so far up to 10,000 hundred million.Wheat bran can not only provide some nutritional labelings by being cultivated bacterium, also because its network structure has bigger specific area, so can adhere to the more thalline of absorption in unit volume.
2) with the use amount of every fowl 1 gram bacteria preparation every day, after other feed mixes, feed egg fowl after 3～5 days, the ight soil that fowl is discharged does not almost have stink, and the fly mosquito reduces, and the pouity dwelling place environmental sanitation is obviously improved.Birds, beasts and eggs brightness, hardness increase, and reduce breakage rate 1%; Laying rate can improve 10～13%, and feed conversion rate improves 8～12%.The incidences of disease such as diarrhoea bacillary dysentery that cause because of Escherichia coli obviously reduce, and no influenza takes place.Obviously strengthen the immunity and the resistance against diseases of supporting fowl.
Good effect of the present invention also is:
1. after bacteria preparation of the present invention is activated, can the little ecological dominance flora of very fast formation in the stomach of fowl, with its degradation of organic substances of the collaborative performance of former indigenous bacterium symbiosis, shortly give birth to, effect that benefit is given birth to.
2. because the ight soil that fowl is discharged does not almost have stink, not only improved the sanitary condition of pouity dwelling place and also improved plant's sanitary condition on every side.The direct compostization of poultry manure of feeding probio is converted into the senior organic fertilizer of producing green agricultural product.
3. promote the metabolism of fowl, improve the conversion ratio of feed, promote the growth of young fowl, increase the nutritive value of laying rate and birds, beasts and eggs, increase the hardness and the brightness of egg.Utilize the inactive yeast bacterium that polysaccharide, albumen, B family vitamin and mineral matter are provided; Utilize the antibiotics of generations such as actinomyces, promote the growth of beneficial bacterium, suppress harmful germ, improve the immunity of fowl, enhancing anti influenza, antiviral ability.Owing to improved the ability of fowl absorption, digesting protein and carbohydrate, so the ight soil of discharging does not have stink, obviously improved the environmental quality of pouity dwelling place, promote the healthy growth of young fowl and egg fowl.
The specific embodiment
Embodiment 1
Follow these steps to carry out the preparation of bacteria preparation:
1), shakes bottle respectively and cultivate (200ml～2L) with each strain inclined plane activation of 4 ℃ of preservations.Bacillus, saccharomycete and actinomyces are used the soluble starch medium culture, and it is as follows specifically to fill a prescription: soluble starch 20g, KNO
31g, NaCl 0.5g, K
2HPO
40.5g, MgSO
4.7H2O 0.5g, FeSO
40.01g, agar 15～20g, distilled water 1000ml.pH7.3,121 ℃ of autoclavings 20 minutes.Lactic acid bacteria is directly cultivated with the sterilization fresh cow milk.Bacillus and saccharomycete were cultivated 22 hours down at 30 ℃; Actinomyces and lactic acid bacteria were cultivated 48 hours for 30 ℃.
2) level liquid enlarges cultivation (independent respectively expansion of bacillus, saccharomycete, actinomyces and lactic acid bacteria cultivated, and culture medium and condition of culture are the same) (20L jar), inoculum concentration 15%.
3) secondary liquid enlarges cultivation (200L jar), inoculum concentration 15% respectively.
4) with wheat bran be carrier respectively solid enlarge to cultivate and be immobilized onto on the wheat bran.
5) will enlarge the saccharomycete of cultivating and in about 75 ℃, heat deactivation in 45 minutes; Its excess-three class enlarges the bacterium of cultivation in 40 ℃ of dryings, pulverizing.
All kinds of bacterium are in following ratio proportioning: bacillus 400 grams, and actinomyces 300 grams, lactic acid bacteria 300 grams mix, and sneak into the saccharomycete of 5% deactivation simultaneously, after the assay was approved, are sealed in the Polythene Bag room temperature preservation.
Embodiment 2
With the bacteria preparation of above-mentioned preparation in following ratio proportioning: 600 kilograms of bacillus, 200 kilograms in actinomyces, the saccharomycete of 10% deactivation is sneaked in 200 kilograms of mixing of lactic acid bacteria simultaneously, after the assay was approved, is sealed in the Polythene Bag room temperature preservation.
Claims (2)
1. a stud bird is used the probiotics bacterial preparation, the main mixed bacterial of forming by bacillus, actinomyces, lactic acid bacteria, the saccharomycete that adds deactivation again, wherein the percentage by weight of all kinds of bacterium is: bacillus 50～70%, actinomyces 10～30%, lactic acid bacteria 20～40%, inactive yeast bacterium 5～10%.
2. a stud bird comprises the steps: with the preparation method of probiotics bacterial preparation
1) each slant strains of activation preservation is shaken bottle respectively and is cultivated, and saccharomycete, bacillus and actinomyces are used the soluble starch medium culture, pH7.0～7.5,100～140 ℃ autoclaving 20-30 minute; Lactic acid bacteria is directly cultivated with the sterilization fresh cow milk; Bacillus was cultivated 20～24 hours for 30～37 ℃; Actinomyces and lactic acid bacteria were cultivated 48～50 hours for 28～30 ℃;
2) saccharomycete, bacillus, the level liquid of actinomyces and lactic acid bacteria enlarge cultivates (20L jar); Inoculum concentration 10%～20%;
Saccharomycete, bacillus, the secondary fluid enlargement culture of actinomyces and lactic acid bacteria (200L jar); Inoculum concentration 10%～20%;
With wheat bran be carrier respectively solid enlarge and cultivate and be immobilized onto on the wheat bran;
With saccharomycete in 75 ℃ of left and right sides deactivations 45 minutes, with bacillus, actinomyces and lactic acid bacteria dry below 30～40 ℃, pulverize;
Bacillus, actinomyces and lactic acid bacteria are mixed by following proportioning: bacillus 50～70%, actinomyces 10～30%, lactic acid bacteria 20～40% adds the saccharomycete of 5～10% deactivation simultaneously again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100203873A CN1596693A (en) | 2004-08-31 | 2004-08-31 | Beneficial bacteria preparation for poultry and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100203873A CN1596693A (en) | 2004-08-31 | 2004-08-31 | Beneficial bacteria preparation for poultry and its preparation method |
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| Publication Number | Publication Date |
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| CN1596693A true CN1596693A (en) | 2005-03-23 |
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| CNA2004100203873A Pending CN1596693A (en) | 2004-08-31 | 2004-08-31 | Beneficial bacteria preparation for poultry and its preparation method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
| CN102524538A (en) * | 2012-01-11 | 2012-07-04 | 陈文� | Feed leavening agent and fermented feed for promoting growth and liver development of geese |
| CN104430173A (en) * | 2014-12-15 | 2015-03-25 | 新疆农业大学 | Feeding method applicable to supplementarily feeding microbial preparations for breast-fed foals |
| CN105016483A (en) * | 2015-07-08 | 2015-11-04 | 莆田市秀屿区汇海水产养殖专业合作社 | Prevention and treatment method for enteromorpha prolifera |
| CN106488705A (en) * | 2014-05-09 | 2017-03-08 | 海凯尔保健食品私人有限公司 | Mitigate the digestion supplement of untoward reaction |
-
2004
- 2004-08-31 CN CNA2004100203873A patent/CN1596693A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
| CN102008016B (en) * | 2010-11-09 | 2013-06-19 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
| CN102524538A (en) * | 2012-01-11 | 2012-07-04 | 陈文� | Feed leavening agent and fermented feed for promoting growth and liver development of geese |
| CN102524538B (en) * | 2012-01-11 | 2013-05-01 | 陈文� | Feed leavening agent and fermented feed for promoting growth and liver development of geese |
| CN106488705A (en) * | 2014-05-09 | 2017-03-08 | 海凯尔保健食品私人有限公司 | Mitigate the digestion supplement of untoward reaction |
| CN104430173A (en) * | 2014-12-15 | 2015-03-25 | 新疆农业大学 | Feeding method applicable to supplementarily feeding microbial preparations for breast-fed foals |
| CN105016483A (en) * | 2015-07-08 | 2015-11-04 | 莆田市秀屿区汇海水产养殖专业合作社 | Prevention and treatment method for enteromorpha prolifera |
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