CN102511440A - Non-resistance cultivation method with combined application of strain for disinfection and biological isolation - Google Patents
Non-resistance cultivation method with combined application of strain for disinfection and biological isolation Download PDFInfo
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- CN102511440A CN102511440A CN2011102993570A CN201110299357A CN102511440A CN 102511440 A CN102511440 A CN 102511440A CN 2011102993570 A CN2011102993570 A CN 2011102993570A CN 201110299357 A CN201110299357 A CN 201110299357A CN 102511440 A CN102511440 A CN 102511440A
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- bacterial classification
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Abstract
The invention belongs to the field of animal husbandry and specifically relates to a non-resistance cultivation method with combined application of a strain for disinfection and biological isolation. The non-resistance cultivation method is characterized by comprising the following two aspects: (1) spraying a bacterial suspension for disinfection in an environment; and (2) planting afforestation trees and Chinese herbal medicines with a disinfection role for replacing isolation strips between adjacent colony houses. The non-resistance cultivation method provided by the invention can obviously improve a rearing environment, improve cultivation income, reduce rearing cost and cultivation risk and provide a strong guarantee for getting high-quality non-resistance products.
Description
Technical field
The invention belongs to the livestock breeding field, be specifically related to a kind of the sterilization with bacterial classification and biological nonreactive cultural method of isolating Combined application.
Background technology
Along with the continuous expansion of breeding scale level and the raising of world agriculture economic globalization degree.China's livestock breeding faces unprecedented development space and huge challenge for existence.At present, China's livestock breeding exists many problems and difficulty, and for example: requiring of (1) human major product and by product to livestock breeding is increasingly high; (2) eqpidemic disease is more and more serious to the threat of the health of animal population and breeding environment; (3) the crop products price is constantly soaring, causes livestock breeding pressure increasing; (4) the international trade barrier influences the fast development of China's livestock breeding; (5) the breed pollution problem is more and more outstanding, becomes the bottleneck of herding enterprise development, and has caused the great attention of society and government; (6) abuse of antibiotic etc not only to animal, even causes bigger hidden danger to health.
The existence of these problems has produced tremendous influence to livestock breeding, so herding enterprise all begins actively to seek and a kind ofly can improve animal welfare, reduce environmental pollution, can improve the cultural method of consumption safety again.
At present, in livestock breeding is cultured, add useful microorganism species and become a scientific and reasonable technology, but these bacterial classifications of how arranging in pairs or groups have become the project that each enterprise falls over each other to study.
The EM bacterium is that a kind of Mixed Microbes generally comprises sporeformer, saccharomycete, actinomycetes, azotobacter, cellulose decomposition bacteria, lactic acid bacteria etc., cultivates the effectively compound microbial flora that forms through specific process.Can be used for food and add, culture disease control, waste water control etc.
Summary of the invention
The purpose of this invention is to provide a kind of the sterilization with bacterial classification and biological nonreactive cultural method of isolating Combined application; Can bacterial classification be carried out reasonably combined and then is applied in the link of breed; And take biological the isolation to make that whole breed is more scientific and reasonable; Avoid the eqpidemic disease and the problem of environmental pollution that occur in the breeding process, improve the quality of livestock breeding major product and by product.
A kind of sterilization with bacterial classification and biological nonreactive cultural method of isolating Combined application of the present invention comprises following two aspects:
(1) will sterilize that to use bacterial classification to process concentration be that bacteria suspension use in the sterilization of 50～10,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 50～100 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(2) plantation landscape tree and the Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
Wherein, the landscape tree in aspect (2) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree, and Chinese herbal medicine is preferably honeysuckle.Landscape tree of being planted and Chinese herbal medicine are isolated the transfer barrier that forms daily pathogen diffusion as biology; Guarantee that occurring in eqpidemic disease that few generation between the letting animals feed of back even do not take place with air is infectious disease popular of main route of transmission, avoids as far as possible even stop the cross-infection between the letting animals feed eqpidemic disease.
The present invention does not add any antibiotic in whole breeding process, reduce aquaculture cost, and avoided the healthy hidden danger of antibiotic to animal and human's body.
The invention has the advantages that: in breeding process; More than two aspect Combined application; Add scientific and reasonable feeding and management; Guaranteeing that letting animals feed obtains under the condition of suitable growth temperature, suppressing to be harmful to growing of bacterium in the feeding environment, reducing ill probability through the advantageous effect of environment disinfected with microorganism.The air borne approach of the plantation pathogenic microorganism capable of blocking through landscape tree and Chinese herbal medicine is being avoided importing into and reducing to reduce aspect the cross-infection and culture risk of eqpidemic disease.The present invention has obviously improved feeding environment, has improved the breed income, reduced feeding cost and has cultured risk, for obtaining high-quality nonreactive product powerful guarantee is provided.
Embodiment
Below in conjunction with instance the present invention is further specified.
Embodiment 1:
Present embodiment is an example with the feeding of broiler, comprises following 2 aspects:
(1) will sterilize that to use bacterial classification to be mixed with concentration be that bacteria suspension use in the sterilization of 7,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 80 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(2) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
Each item achievement data that present embodiment obtains is following:
(1) general index:
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.7∶1 | 2.45Kg | 93.3％ | 0 | Do not have |
(2) the residual detection of medicine: (detecting index is standard with the weight of animals)
| Sequence number | Project | Result (μ g/Kg) | Sequence number | Project | Result (μ g/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | 0 |
| 2 | Sulfanilamide (SN) (15) | 0 | 10 | Quinolone (13) | 0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | 0 |
| 4 | Chloramphenicol | 0 | 12 | Ractopamine | 0 |
| 5 | Nitrofurazone | 0 | 13 | ROX | 0 |
| 6 | Furaltadone | 0 | 14 | Tylosin | 0 |
| 7 | Furantoin | 0 | 15 | Lincomycin | 0 |
| 8 | Furazolidone | 0 | 16 | Erythromycin | 0 |
Embodiment 2:
It is example that present embodiment is raised with the meat duck, comprises following 2 aspects:
(1) will sterilize that to use bacterial classification to be mixed with concentration be that bacteria suspension use in the sterilization of 8,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 70 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(2) alternately plant the isolation strip that Chinese wax, method paulownia, Chinese scholar tree, honeysuckle replace the adjacent turn my humble abode.
Each item achievement data that present embodiment obtains is following:
(1) general index
| Project | Breeding cycle | Feedstuff-meat ratio | Deliver average weight for sale | Survival rate | Case fatality rate | Antibiotic uses |
| Numerical value | 42d | 1.87∶1 | 2.85Kg | 93.1％ | 0 | Do not have |
(2) the residual detection of medicine: (detecting index is standard with the weight of animals)
| Sequence number | Project | Result (ug/Kg) | Sequence number | Project | Result (ug/Kg) |
| 1 | Nitroimidazole | Do not detect | 9 | Clenbuterol | ?0 |
| 2 | Sulfanilamide (SN) (15) | ?0 | 10 | Quinolone (13) | ?0 |
| 3 | Tetracycline (4) | Do not detect | 11 | Salbutamol | ?0 |
| 4 | Chloramphenicol | ?0 | 12 | Ractopamine | ?0 |
| 5 | Nitrofurazone | ?0 | 13 | ROX | ?0 |
| 6 | Furaltadone | ?0 | 14 | Tylosin | ?0 |
| 7 | Furantoin | ?0 | 15 | Lincomycin | ?0 |
| 8 | Furazolidone | ?0 | 16 | Erythromycin | ?0 |
Claims (3)
1. sterilize with bacterial classification and biological nonreactive cultural method of isolating Combined application for one kind, it is characterized in that comprising following two aspects:
(1) will sterilize that to use bacterial classification to process concentration be that bacteria suspension use in the sterilization of 50～10,000,000,000 viable bacterias/mL, then sterilization with bacteria suspension according to 1: 50～100 quality than thin up, spray disinfectant in feeding environment wherein, is sterilized with bacterial classification employing EM bacterium at last;
(2) plantation landscape tree and the Chinese herbal medicine with disinfective action replace the isolation strip of adjacent turn my humble abode.
2. sterilization according to claim 1 is with bacterial classification and biological nonreactive cultural method of isolating Combined application, it is characterized in that landscape tree in the aspect (2) is preferably one or more in Chinese wax, method paulownia or the Chinese scholar tree.
3. sterilization according to claim 1 is characterized in that with bacterial classification and biological nonreactive cultural method of isolating Combined application the Chinese herbal medicine in the aspect (2) is preferably honeysuckle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102993570A CN102511440A (en) | 2011-09-29 | 2011-09-29 | Non-resistance cultivation method with combined application of strain for disinfection and biological isolation |
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| CN2011102993570A CN102511440A (en) | 2011-09-29 | 2011-09-29 | Non-resistance cultivation method with combined application of strain for disinfection and biological isolation |
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| CN102511440A true CN102511440A (en) | 2012-06-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577325A (en) * | 2016-11-25 | 2017-04-26 | 南宁市浩特竹鼠养殖场 | Application method of EM bacteria to cultivation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101760431A (en) * | 2010-01-05 | 2010-06-30 | 北京市农林科学院 | Compound microbial culture starter and application thereof |
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
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2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760431A (en) * | 2010-01-05 | 2010-06-30 | 北京市农林科学院 | Compound microbial culture starter and application thereof |
| CN102008016A (en) * | 2010-11-09 | 2011-04-13 | 南京农业大学 | Probiotic solid powder and preparation method thereof |
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| HUYNH A.HONG ET AL: "The use of bacterial spore formers as probiotics", 《FEMS MICROBIOLOGY REVIEWS》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577325A (en) * | 2016-11-25 | 2017-04-26 | 南宁市浩特竹鼠养殖场 | Application method of EM bacteria to cultivation |
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Application publication date: 20120627 |