CN102379364B - Non-resistance cultivation method with joint application of feed strain and drinking water strain - Google Patents

Abstract

The invention belongs to the field of animal husbandry and specifically relates to a non-resistance cultivation method with joint application of feed strain and drinking water strain. It is characterized in the following two aspects: (1) adding feed strain to feed; and (2) adding drinking water strain to drinking water. The invention obviously improves the feeding environment, increases the cultivation income, reduces the feed cost and cultivation risk, and provides guarantee to production of high-quality non-resistance products.

Description

The nonreactive cultivation method of feed spawn and drinking water bacterial classification use in conjunction

Technical field

The invention belongs to the animal husbandry field, be specifically related to the nonreactive cultivation method of a kind of feed spawn and drinking water bacterial classification use in conjunction.

Background technology

Along with the continuous expansion of breeding scale level and the raising of world agriculture economic globalization degree.China's animal husbandry faces unprecedented development space and huge challenge for existence.At present, there are many problems in China's animal husbandry, and for example: (1) human major product and requirement of byproduct to animal husbandry is more and more higher; (2) epidemic disease is more and more serious to the threat of the health of animal population and breeding environment; (3) the crop products price is constantly soaring, causes animal husbandry pressure increasing; (4) the international trade barrier affects the fast development of China's animal husbandry; (5) the breeding pollution problem is more and more outstanding, becomes the bottleneck of Animal husbandry development, and has caused the great attention of society and government; (6) abuse of antibiotic etc not only to animal, even causes larger hidden danger to health.

The existence of these problems has produced tremendous influence to animal husbandry, so Animal husbandry all begins actively to seek and a kind ofly can improve animal welfare, reduce environmental pollution, can improve again the cultural method of consumption safety.

At present, in the animal husbandry cultivation, add useful microorganism species and become a scientific and reasonable technology, but these bacterial classifications of how arranging in pairs or groups have become the project that each enterprise falls over each other to study.

Bacillus licheniformis cellular morphology and arrangement are shaft-like, Dan Sheng, capable of regulating flora (Bifidobacterium Bifidum, Bacillus acidi lactici, bacteroid, peptostreptococcus are had the promotion growth) imbalance reaches therapeutic purposes, can impel body to produce antibacterial substance, kill pathogenic bacteria (pathogenic bacteria such as staphylococcus, yeast-like fungus are had antagonism).Can produce the resistant activity material, and have unique biology and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria.

Bacillus subtilis is a kind of of bacillus.The subtilin that produces in the bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis consumes rapidly the free oxygen in the environment, causes the enteron aisle hypoxemia, promotes the growth of useful anaerobic bacteria, and produces the organic acid such as lactic acid, reduces the enteron aisle pH value, indirectly suppresses other pathogenic bacteria growth.Thalline self synthesizes the enzymes such as AMS, protease, lipase, cellulase, in alimentary canal with animal body in digestive enzymes together play a role; The multiple B family vitamins such as energy synthetic vitamin B1, B2, B6, nicotinic acid, the activity of interferon and macrophage in the raising animal body.

Candida tropicalis is without O ₂The time, decompose monose and produce CO ₂And energy; O is arranged ₂The time, monose thoroughly is decomposed into CO ₂And H ₂O.Candida tropicalis has very high nutritive value, particularly contains more protein, and a lot of B family vitamins, nucleic acid and mineral matter also can produce some health care active materials simultaneously.The candida tropicalis fermentation culture medium has the interferon of enhancing to tire, thereby the enhanced machine body immunity function is arranged.

Lactobacillus acidophilus belongs to lactobacillus, Gram-positive bacillus, and bar terminal rounded mainly exists in the small intestine, adjusts the gut flora balance, suppresses the propagation of enteron aisle undesirable microorganism.Lactobacillus acidophilus has antagonism to pathogenic microorganisms.Lactobacillus acidophilus also can produce lactic acid and acetic acid after fermenting in enteron aisle, can improve the utilization rate of calcium, phosphorus, iron, promote the absorption of iron and vitamin D, produce vitamin K and Cobastab, can also reduce the absorption of cholesterol, and can reduce the injury of Radiation On Human body.

Bafillus natto is to have beneficial bacterium acidproof, heat-resistant quality; four hours survival rates are 100% under hydrochloric acid in gastric juice; have simultaneously powerful pathogen and suppress ability; to environment tolerance one of the bacterial classification of small intestine that preferably can go directly in the middle of the various beneficial bacterium; it is ecological to change the animal intestinal flora after oral; help digest function normalization, so that defecation is smooth and easy, keep the body physiological environmental protection.Can produce acid, the regulating intestinal canal flora, the enhancing animal cell immunity is put should.And can generate multiple protein enzyme (particularly alkali protease), carbohydrase, lipase, amylase, some complicated carbohydrate of degrading plant forage kind, thereby the conversion ratio of raising feed.

Streptococcus fecalis is again enterococcus faecalis.Use the C polysaccharide antigen, according to the Lan Shi serological classification, streptococcus can be divided into many groups.Streptococcus fecalis belongs to D group, and the D group streptococcus divides enterococcus and non-enterococcus two classes.The former comprises streptococcus fecalis, streptococcus faecalis and streptococcus durans, and the latter has bargen's streptococcus and streptococcus equinus.Streptococcus fecalis is additive for farm animal feed, can prevent and treat the cub fledgling poultry because of the diarrhea that intestinal bacilli illness causes, the effect that has the imbalance of regulating intestinal canal normal flora and promote to grow.

Bifidobacterium can be safeguarded enteron aisle normal bacteria colony balance, suppresses the growth of pathogen, prevents constipation, diarrhea and gastrointestinal disorders etc.Energy synthetic vitamin, amino acid and raising body reduce the Blood Cholesterol level to the absorption of calcium ion in enteron aisle, prevent and treat hypertension, and improve the anti-lactose of dairy products, improve digestibility.Safeguard enteron aisle normal bacteria colony balance, suppress the growth of pathogen, have the disorderly effect of the function of intestinal canal of adjustment; Synthetic vitamin, amino acid and raising body are to the absorption of calcium ion in enteron aisle; Strengthen the human immunologic function, prevent antibiotic side effect.

Summary of the invention

The nonreactive cultivation method that the purpose of this invention is to provide a kind of feed spawn and drinking water bacterial classification use in conjunction, can carry out each bacterial classification reasonably combined and then be applied in the links of cultivation, avoid the epidemic disease problem that occurs in the breeding process, improve the quality of animal husbandry major product and byproduct.

The nonreactive cultivation method of a kind of feed spawn of the present invention and drinking water bacterial classification use in conjunction comprises following two aspects:

(1) according to the standard that adds 2～4kg feed spawn in the feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;

(2) the drinking water bacterial classification is made the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 80～160 quality, maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body, in the daily drinking water of biology, add the drinking water bacteria suspension, wherein, according to the parts by weight meter, the drinking water bacterial classification is by 2～3 parts of lactobacillus acidophilus, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium are formulated.

Wherein, in aspect (2), hour can suitably improve drinking-water with the dilution ratio of bacteria suspension at institute's domesticated animal amount of drinking water, then will suitably turn down drinking-water when opposite amount of drinking water is larger with the dilution ratio of bacteria suspension.

The present invention does not add any antibiotic in whole breeding process, reduce aquaculture cost, and avoided the healthy hidden danger of antibiotic to animal and human's body.

The invention has the advantages that: in breeding process, more than two aspect use in conjunction, add scientific and reasonable feeding and management, guaranteeing that letting animals feed obtains under the condition of suitable growth temperature, with the growing of harmful bacteria in the advantageous effect of the microorganism inhibition feeding environment, reduce ill probability by drinking-water and feed.The present invention has obviously improved feeding environment, has improved the cultivation income, has reduced feeding cost and cultivation risk, provides powerful guarantee for obtaining high-quality nonreactive product.

The specific embodiment

The present invention will be further described below in conjunction with example.

Embodiment 1:

Present embodiment comprises following 2 aspects take feeding of broiler as example:

(1) according to the standard that adds the 4kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 5,500,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis;

(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 6,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 100 quality, beneficial bacterium valid density maintains the standard of 200,000,000 viable bacterias/kg in according to animal body, in biological drinking water, add the drinking water bacteria suspension after diluting, wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 3 parts of lactobacillus acidophilus, 3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 1 part of bafillus natto, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums.

The indices data that present embodiment obtains are as follows:

(1) general index:

Project

Breeding cycle

Feedstuff-meat ratio

Deliver average weight for sale

Survival rate

Case fatality rate

Antibiotic uses

Numerical value

42d

1.68∶1

2.42Kg

93.1％

0

Nothing

(2) the residual detection of medicine: (detecting index take the weight of animals as standard)

Sequence number	Project	Result (μ g/Kg)	Sequence number	Project	Result (μ g/Kg)
						1	Nitroimidazole	Do not detect	9	Clenbuterol	0
2	Sulfanilamide (SN) (15)	0	10	Quinolone (13)	0
						3	Tetracycline (4)	Do not detect	11	Salbutamol	0
4	Chloramphenicol	0	12	Ractopamine	0
						5	Nitrofurazone	0	13	ROX	0
6	Furaltadone	0	14	Tylosin	0
						7	Furantoin	0	15	Lincomycin	0
8	Furazolidone	0	16	Erythromycin	0

Embodiment 2:

Present embodiment is raised as example take the meat duck, comprises following 2 aspects:

(1) according to the standard that adds the 4kg feed spawn in the feed per ton, add feed spawn in feed, wherein, the bacterial classification content of feed spawn is 9,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 3 parts of bacillus licheniformis and 1 part of bacillus subtilis;

(2) the drinking water bacterial classification is mixed with the drinking water bacteria suspension that concentration is 9,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 100 quality, beneficial bacterium valid density maintains the standard of 200,000,000 viable bacterias/kg in according to animal body, in biological drinking water, add the drinking water bacteria suspension after diluting, wherein, according to the parts by weight meter, the drinking water bacterial classification is formulated by 3 parts of lactobacillus acidophilus, 3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2 portions of candida tropicalises, 1 part of bafillus natto, 1 part of streptococcus fecalis and 2 parts of Bifidobacteriums.

The indices data that present embodiment obtains are as follows:

(1) general index:

Project

Breeding cycle

Feedstuff-meat ratio

Deliver average weight for sale

Survival rate

Case fatality rate

Antibiotic uses

Numerical value

42d

1.88∶1

2.87Kg

93.6％

0

Nothing

(2) the residual detection of medicine: (detecting index take the weight of animals as standard)

Sequence number	Project	Result (μ g/Kg)	Sequence number	Project	Result (μ g/Kg)
						1	Nitroimidazole	Do not detect	9	Clenbuterol	0
2	Sulfanilamide (SN) (15)	0	10	Quinolone (13)	0
						3	Tetracycline (4)	Do not detect	11	Salbutamol	0
4	Chloramphenicol	0	12	Ractopamine	0
						5	Nitrofurazone	0	13	ROX	0
6	Furaltadone	0	14	Tylosin	0
						7	Furantoin	0	15	Lincomycin	0
8	Furazolidone	0	16	Erythromycin	0

Claims (1)

1. the nonreactive cultivation method of a feed spawn and drinking water bacterial classification use in conjunction is characterized in that comprising following two aspects:

(1) according to the standard that adds 2～4kg feed spawn in the fermented feed per ton, in feed, add feed spawn, wherein, the bacterial content of feed spawn is 50～10,000,000,000 viable bacterias/g, according to the parts by weight meter, feed spawn is formulated by 2～3 parts of bacillus licheniformis and 1 part of bacillus subtilis;

(2) the drinking water bacterial classification is made the drinking water bacteria suspension that concentration is 50～10,000,000,000 viable bacterias/mL, then the drinking water bacteria suspension compares thin up according to 1: 80～160 quality, maintain the standard of 1.5～300,000,000 viable bacterias/kg according to beneficial bacterium valid density in the animal body, in the daily drinking water of biology, add the drinking water bacteria suspension, wherein, according to the parts by weight meter, the drinking water bacterial classification is by 2～3 parts of lactobacillus acidophilus, 2～3 parts of bacillus licheniformis, 1 part of bacillus subtilis, 2～3 portions of candida tropicalises, 1～2 part of bafillus natto, 1 part of streptococcus fecalis and 1～2 part of Bifidobacterium are formulated.