CN106578351A - Feed additive for increasing lean meat percentage as well as preparation method and application of feed additive - Google Patents
Feed additive for increasing lean meat percentage as well as preparation method and application of feed additive Download PDFInfo
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- CN106578351A CN106578351A CN201611022446.XA CN201611022446A CN106578351A CN 106578351 A CN106578351 A CN 106578351A CN 201611022446 A CN201611022446 A CN 201611022446A CN 106578351 A CN106578351 A CN 106578351A
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Abstract
The invention discloses a feed additive for increasing lean meat percentage as well as a preparation method and application of the feed additive. The feed additive comprises the following components: a bacillus pumilus bacterium agent, a lactobacillus casei bacterium agent and an enterococcus faecium bacterium agent. The invention further discloses a preparation method of the feed additive; the preparation method comprises the following steps: (1) respectively mixing the bacillus pumilus bacterium agent, the lactobacillus casei bacterium agent and the enterococcus faecium bacterium agent with auxiliary materials to prepare a bacillus pumilus bacterium agent premix, a lactobacillus casei bacterium agent premix and an enterococcus faecium bacterium agent premix; (2) mixing the above premixes uniformly to obtain the feed additive. The feed additive provided by the invention is free of antibiotics, simple in preparation process, wide in source of raw materials, low in cost, economical and practical, and free of residue and toxic side effect, can effectively improve quality of pork, reduces backfat thickness of the pork, and increases the lean meat percentage.
Description
Technical field
The present invention relates to a kind of feed additive for improving lean meat percentage, more particularly to improve the feedstuff of lean meat percentage of Carnis Sus domestica and add
Plus agent, the invention further relates to the preparation method of the feed additive, belongs to feed additive field.
Background technology
Carnis Sus domestica is the main meat product that people obtain protein and energy, very popular.With modern people
Growth in the living standard and the enhancing to health perception, quality requirements more and more higher of the people to Carnis Sus domestica.It is higher in order to pursue
Lean meat percentage, the main method for adopting at present is to add the lean meat species additives such as clenbuterol hydrochloride, antibiotic, growth hormone in feedstuff.
But use of the medicines such as clenbuterol hydrochloride, antibiotic, hormone in feedstuff has serious negative effect, not only causes domestic animal intestinal
Flora Disturbance, bacterial drug resistance strengthen, cause anaphylaxiss or toxic and side effects, cause disease to be upgraded, and have also had a strong impact on animal
Product quality and edible safety, directly affect human health, meanwhile, also result in drug residue and environmental pollution.
Therefore, R and D go out to effectively improve the feed additive of the safety without hidden danger of pig lean meat percentage, to pig industry
Development is significant.
The content of the invention
An object of the present invention is to provide the feed additive that can improve poultry lean meat percentage, and the feed additive has not
Containing antibiotic, preparation process is simple, raw material sources are extensive, the advantage such as drug residue free.
The second object of the present invention is to provide the preparation method of the feed additive.
The third object of the present invention is the application by the feed additive in the cultivation of poultry lean meat percentage is improved.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
The invention discloses a kind of feed additive for improving lean meat percentage, including following components：Bacillus pumilus microbial inoculum,
Lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum.
Preferably, the weight portion of each component is：Bacillus pumilus microbial inoculum 8-16 parts, lactobacillus casei microbial inoculum 5-9 parts, dung
Enterococcus microbial inoculum 3-7 parts；
It is furthermore preferred that the weight portion of each component is：12 parts of Bacillus pumilus microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, dung intestinal
5 parts of coccus microbial inoculum；
Wherein, the viable bacteria content of each component is：Bacillus pumilus are 1.0 × 1011-3.0×1011cfu/g；It is dry
Lactobacillus paracasei is 5.0 × 1011-7.0×1011Cfu/g, enterococcus faecalis are 2.0 × 1011-3.0×1011cfu/g；Preferably：
Bacillus pumilus are 2.0 × 1011cfu/g；Lactobacillus casei is 6.0 × 1011Cfu/g, enterococcus faecalis are 2.5 × 1011cfu/
g。
The present invention has carried out screening test to the component of feed additive.First by Bacillus pumilus microbial inoculum, condense bud
Spore bacillus microbial inoculum, bacillus subtilis microbial agent, bacillus licheniformis agent, five microbial inoculums of Bafillus natt microbial inoculum are respectively prepared
Feed additive, is added in basal diet with 10%, treats feeding three months, butchers, and calculates lean meat percentage and back-fat thickness, as a result
Show the feed additive prepared by Bacillus pumilus microbial inoculum so that the back-fat thickness of Carnis Sus domestica is 2.80cm, than without feeding
Feed additives group reduces 6.8%, and the feed additive prepared significantly lower than other microbial inoculums；Lean meat percentage is 59.2%, than nothing
Feed additive group improves 2.8%, and the feed additive prepared apparently higher than other microbial inoculums；Then by Bacillus pumilus
Microbial inoculum makes feed additive together with lactobacillus casei microbial inoculum, Lactobacillus bulgaricus microbial inoculum, lactobacillus lactiss microbial inoculum respectively,
It is added in basal diet with 10%, treats feeding three months, butcher, calculate lean meat percentage and back-fat thickness, as a result shows by short and small
The feed additive that gemma bacillus agent and lactobacillus casei microbial inoculum are prepared so that the back-fat thickness of Carnis Sus domestica reaches
2.65cm, hence it is evident that less than feed additive prepared by other microbial inoculums；Lean meat percentage is 60.8%, hence it is evident that prepared higher than other microbial inoculums
Feed additive；Finally, by Bacillus pumilus microbial inoculum and lactobacillus casei microbial inoculum respectively with enterococcus faecalis microbial inoculum, middle hammer
Bacteria agent, nisin bacteria agent make together feed additive, are added in basal diet with 10%, treat feeding three months,
Butcher, calculate lean meat percentage and back-fat thickness, as a result show by Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis
Feed additive made by microbial inoculum so that the back-fat thickness of Carnis Sus domestica has reached 2.40cm, hence it is evident that less than feeding prepared by other microbial inoculums
Feed additives；Lean meat percentage is 64.8%, hence it is evident that higher than feed additive prepared by other microbial inoculums.Result above shows short and small spore
The feed additive that bacillus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are prepared can significantly improve the matter of Carnis Sus domestica
Amount, significantly reduces Carnis Sus domestica back-fat thickness, increases lean meat percentage.
On this basis, the probiotic bacteria dosage determination tests that the present invention passes through Three factors-levels, it is determined that the feedstuff adds
Plus the optimum amount of agent each component：12 parts of Bacillus pumilus microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum.
Invention also discloses the preparation method of above-mentioned feed additive, including：(1) by Bacillus pumilus microbial inoculum, dry
Lactobacillus paracasei microbial inoculum, enterococcus faecalis microbial inoculum carry out being mixed with and obtain Bacillus pumilus microbial inoculum pre-mixing agent, do with adjuvant respectively
Lactobacillus paracasei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent；
(2) by Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent
Mix, obtain final product.
Wherein, described adjuvant is the mixture of any one or two kinds of starch and oligosaccharide or levan；Microbial inoculum with it is auxiliary
The mass ratio of material is 1:5-15, preferably 1:10.
The present invention further discloses a kind of feed additive for improving lean meat percentage, including following components：Short and small spore bar
Bacteria agent, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum, Rhizoma Polygoni Cuspidati and Herba Erodii.
Preferably, the weight portion of each component is：Bacillus pumilus microbial inoculum 8-16 parts, lactobacillus casei microbial inoculum 5-9 parts, dung
Enterococcus microbial inoculum 3-7 parts, Rhizoma Polygoni Cuspidati 4-12 parts, Herba Erodii 2-6 parts；
It is furthermore preferred that the weight of each component is divided into 12 parts of Bacillus pumilus microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, dung intestinal
5 parts of coccus microbial inoculum, 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of Herba Erodii；
Wherein, the viable bacteria content of each component is：Bacillus pumilus are 1.0 × 1011-3.0×1011cfu/g；It is dry
Lactobacillus paracasei is 5.0 × 1011-7.0×1011Cfu/g, enterococcus faecalis are 2.0 × 1011-3.0×1011cfu/g；Preferably：
Bacillus pumilus are 2.0 × 1011cfu/g；Lactobacillus casei is 6.0 × 1011Cfu/g, enterococcus faecalis are 2.5 × 1011cfu/
g。
Rhizoma Polygoni Cuspidati is the rhizome of polygonaceae plant Rhizoma Polygoni Cuspidati.Nature and flavor：Hardship, puts down.Indication：Wind-dispelling, dampness removing, removing blood stasis with potent drugs stimulates the menstrual flow.Control wind
Wet bones and muscles pain, jaundice due to damp-heat, stranguria with turbid discharge leukorrhagia, women's amenorrhea, lochiostasises, lump in the abdomen, anal fistula hematochezia.Fall and flutter damage
Wound, scalds, malignant boil tinea.
Herba Erodii be Huai Niuer Seedlings section plant Mang cattle Seedling or Herba Erodii, nepal cranesbill, Siberia geranium and
The herbs with fruit such as Carolina Cranesbill Herb, tuber person's stork grass.Nature and flavor：Toil, puts down.Indication：Wind-dispelling, promoting blood circulation, heat-clearing and toxic substances removing.
Dispelling the wind and dampness pathogens pain, contraction, carbuncle, traumatic injury, enteritis, dysentery.
The present invention is the effect of raising feed additive, is containing 12 parts of Bacillus pumilus microbial inoculum, lactobacillus casei bacterium
Chinese medicinal components are further with the addition of in 7 parts of agent, the feed additive of 5 parts of enterococcus faecalis microbial inoculum.Specially：In Bacillus pumilus
On the basis of 12 parts of microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum, 8 parts of (1) Rhizoma Polygoni Cuspidati, 4 parts of Rhizoma Alismatis are added respectively；
(2) 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of the Rhizoma Anemarrhenae；(3) 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of Pericarpium Citri Reticulatae；(4) 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of Cortex Cinnamomi；(5) 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of the Radix Astragali；
(4) 8 parts of Rhizoma Alismatis, 4 parts of Herba Erodii；(7) 8 parts of the Rhizoma Anemarrhenae, 4 parts of Herba Erodii；(8) 8 parts of Pericarpium Citri Reticulatae, 4 parts of Herba Erodii；(9) 8 parts of Cortex Cinnamomi, old
4 parts of stork grass；(8) 8 parts of the Radix Astragali, 4 parts of Herba Erodii；(11) 8 parts of Rhizoma Polygoni Cuspidati, 4 parts of Herba Erodii make feed additive.As a result show short
Add 8 parts of Rhizoma Polygoni Cuspidati, Herba Erodii 4 on the basis of 12 parts of bacillus pumiluss microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum
The feed additive 12 of part can effectively improve the quality of Carnis Sus domestica so that the back-fat thickness of Carnis Sus domestica has reached 1.94cm, adds than feedstuff
Plus agent 1 reduces 13.9%, and significantly lower than feed additive 2-11；Lean meat percentage has reached 78.9%, is significantly higher than feedstuff and adds
Plus agent 2-11.Bacillus pumilus microbial inoculum described above, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum and Rhizoma Polygoni Cuspidati, Herba Erodii it
Between there is significant synergistic function, its compatibility can significantly increase the quality of Carnis Sus domestica together, reduce the back fat of Carnis Sus domestica
Thickness, improves lean meat percentage.By contrast, by Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum and its
Its several drugses compatibility together after, for increase Carnis Sus domestica quality, reduce Carnis Sus domestica back-fat thickness, improve lean meat percentage effect
On it is unobvious.
The invention also discloses the preparation method of above-mentioned feed additive, including：
(1) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum are carried out respectively with adjuvant mixing system
It is standby to obtain Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent；
(2) Rhizoma Polygoni Cuspidati, Herba Erodii are crushed into obtain Giant Knotweed Rhizome, Herba Erodii powder；
(3) by Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent, enterococcus faecalis microbial inoculum pre-mixing agent,
Giant Knotweed Rhizome, Herba Erodii powder are mixed, and are obtained final product.
Wherein, described adjuvant is the mixture of any one or two kinds of starch and oligosaccharide or levan；Microbial inoculum with it is auxiliary
The mass ratio of material is 1:5-15, preferably 1:10.
It is of the invention further to disclose application of the feed additive in domestic animal lean meat percentage is improved again, wherein, it is described
Domestic animal, including pig, cattle, sheep or horse；Preferably pig.
The microbial inoculum training method that microbial inoculum of the present invention is provided according to commercial sources carries out culture acquisition.
Technical solution of the present invention compared with prior art, has the advantages that：
Antibiotic is not contained in feed additive of the present invention, preparation process is simple, raw material sources are extensive, low cost, it is economical real
With, noresidue, have no toxic side effect；The quality of Carnis Sus domestica can be effectively improved, the lean meat percentage of Carnis Sus domestica is improved.
Specific embodiment
Further describe the present invention with reference to specific embodiment, advantages of the present invention and feature will be with description and
It is apparent.It should be understood that the embodiment is only exemplary, the scope of the present invention is not construed as limiting.Art technology
Personnel should be understood that without departing from the spirit and scope of the invention can be to the details of technical solution of the present invention and form
Modify or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
1st, strain source
Bacillus pumilus microbial inoculum：ACCC 01671 is purchased from Chinese agriculture Culture Collection；
Lactobacillus casei microbial inoculum：ACCC03948 is purchased from Chinese agriculture Culture Collection；
Enterococcus faecalis microbial inoculum：ACCC10705 is purchased from Chinese agriculture Culture Collection.
Microbial inoculum used in the present invention expands according to the formula and cultural method of culture medium given during purchase strain
Increasing is obtained (also can be commercially available from commercial channels).
The preparation of the feed additive of embodiment 1
(1) each component is weighed：Bacillus pumilus microbial inoculum 8kg, lactobacillus casei microbial inoculum 5kg, enterococcus faecalis microbial inoculum 3kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and oligosaccharide, and microbial inoculum is 1 with the mass ratio of adjuvant:5；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of the feed additive of embodiment 2
(1) each component is weighed：Bacillus pumilus microbial inoculum 16kg, lactobacillus casei microbial inoculum 9kg, enterococcus faecalis microbial inoculum 7kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and levan, and microbial inoculum is 1 with the mass ratio of adjuvant:15；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of the feed additive of embodiment 3
(1) each component is weighed：Bacillus pumilus microbial inoculum 12kg, lactobacillus casei microbial inoculum 7kg, enterococcus faecalis microbial inoculum 5kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and oligosaccharide, and microbial inoculum is 1 with the mass ratio of adjuvant:10；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of the feed additive of embodiment 4
(1) each component is weighed：Bacillus pumilus microbial inoculum 8kg, lactobacillus casei microbial inoculum 5kg, enterococcus faecalis microbial inoculum 3kg,
Rhizoma Polygoni Cuspidati 4kg, Herba Erodii 2kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and levan, and microbial inoculum is 1 with the mass ratio of adjuvant:5；
(3) Rhizoma Polygoni Cuspidati, Herba Erodii are crushed, is obtained Giant Knotweed Rhizome, Herba Erodii powder；
(4) by microbial inoculum pre-mixing agent and Giant Knotweed Rhizome, the Herba Erodii powder of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of the feed additive of embodiment 5
(1) each component is weighed：Bacillus pumilus microbial inoculum 16kg, lactobacillus casei microbial inoculum 9kg, enterococcus faecalis microbial inoculum 7kg,
Rhizoma Polygoni Cuspidati 12kg, Herba Erodii 6kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and oligosaccharide, and microbial inoculum is 1 with the mass ratio of adjuvant:15；
(3) Rhizoma Polygoni Cuspidati, Herba Erodii are crushed, is obtained Giant Knotweed Rhizome, Herba Erodii powder；
(4) by microbial inoculum pre-mixing agent and Giant Knotweed Rhizome, the Herba Erodii powder of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of the feed additive of embodiment 6
(1) each component is weighed：Bacillus pumilus microbial inoculum 12kg, lactobacillus casei microbial inoculum 7kg, enterococcus faecalis microbial inoculum 5kg,
Rhizoma Polygoni Cuspidati 8kg, Herba Erodii 4kg；
(2) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are mixed respectively with adjuvant
Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are prepared, wherein,
Adjuvant used is the mixture of starch and oligosaccharide, and microbial inoculum is 1 with the mass ratio of adjuvant:10；
(3) Rhizoma Polygoni Cuspidati, Herba Erodii are crushed, is obtained Giant Knotweed Rhizome, Herba Erodii powder；
(4) by microbial inoculum pre-mixing agent and Giant Knotweed Rhizome, the Herba Erodii powder of above-mentioned preparation, mix homogeneously is obtained final product.
The probiotic composition screening experiment of the feed additive of experimental example 1
1st, experimental technique
1.1 one-components are screened
By Bacillus pumilus microbial inoculum, Bacillus coagulans microbial inoculum, bacillus subtilis microbial agent, Bacillus licheniformis
Agent, Bafillus natt microbial inoculum prepare respectively 5 kinds of feed additives with adjuvant, wherein, adjuvant used is starch and oligomerization
The mixture of sugar, microbial inoculum is 1 with the mass ratio of adjuvant:10.Selection age in days is close to, body weight is close, be in a good state of health piglet
240, designed using completely randomized experiment, be divided into 6 groups, 40 per group.By the feed additive for preparing with 10% ratio
Example is added in the basal diet of pig, and above-mentioned piglet is fed respectively, and one of which only feeds basal diet, waits to feed as control
Three months, butcher, calculate lean meat percentage and back-fat thickness.
1.2 compounding ingredients screenings two-by-two
By Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, Lactobacillus bulgaricus microbial inoculum, lactobacillus lactiss microbial inoculum point
Microbial inoculum pre-mixing agent is not prepared with adjuvant, wherein, adjuvant used is the matter of the mixture of starch and oligosaccharide, microbial inoculum and adjuvant
Amount ratio is 1:10, then by Bacillus pumilus microbial inoculum pre-mixing agent respectively with lactobacillus casei microbial inoculum, Lactobacillus bulgaricus
Microbial inoculum, the pre-mixing agent of lactobacillus lactiss microbial inoculum are with 1:1 ratio is mixed and made into feed additive, and selection age in days is close to, body weight phase
Closely, be in a good state of health piglet 240, is designed using completely randomized experiment, is divided into 6 groups, 40 per group.By the feeding for preparing
Feed additives are added in the basal diet of pig with 10% ratio, and above-mentioned piglet is fed respectively, and one of which is used as control
Feeding basal diet, treats feeding three months, butchers, and calculates lean meat percentage and back-fat thickness.
1.3 33 compounding ingredients are screened
By Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum, middle streptococcus microbial inoculum, lactic acid chain
Coccus microbial inoculum prepares respectively microbial inoculum pre-mixing agent with adjuvant, wherein, adjuvant used is starch and the mixture of oligosaccharide, microbial inoculum
1 is with the mass ratio of adjuvant:10, then Bacillus pumilus microbial inoculum pre-mixing agent and lactobacillus casei microbial inoculum pre-mixing agent are distinguished
With enterococcus faecalis microbial inoculum, middle streptococcus microbial inoculum, nisin bacteria agent pre-mixing agent with 1:1:1 ratio is mixed and made into feedstuff
Additive, selection age in days is close to, body weight is close, be in a good state of health piglet 240, is designed using completely randomized experiment, is divided into 6
Group, 40 per group.The feed additive for preparing is added in the basal diet of pig with 10% ratio, respectively in feeding
Piglet is stated, one of which only feeds basal diet, treat feeding three months, butchers as control, calculates lean meat percentage and back-fat thickness.
2nd, experimental result
2.1 one-components are screened
As can be seen from Table 1：DIFFERENT FEED additive has material impact to the lean meat percentage and back-fat thickness of Carnis Sus domestica.By
The feed additive that Bacillus pumilus microbial inoculum is prepared so that the back-fat thickness of Carnis Sus domestica is 2.80cm, than adding without feedstuff
Agent group reduces 6.8%, and the feed additive prepared significantly lower than other microbial inoculums；Lean meat percentage is 59.2%, than adding without feedstuff
Plus agent group improves 2.8%, and the feed additive prepared apparently higher than other microbial inoculums.
Impact of the DIFFERENT FEED additive of table 1 to meat quality
2.2 compounding ingredients screenings two-by-two
As can be seen from Table 2：DIFFERENT FEED additive has material impact to the lean meat percentage and back-fat thickness of Carnis Sus domestica.By
The feed additive that Bacillus pumilus microbial inoculum and lactobacillus casei microbial inoculum are prepared so that the back-fat thickness of Carnis Sus domestica reaches
2.65cm, hence it is evident that less than feed additive prepared by other microbial inoculums；Lean meat percentage is 60.8%, hence it is evident that prepared higher than other microbial inoculums
Feed additive.
Impact of the DIFFERENT FEED additive of table 2 to meat quality
1.3 33 compounding ingredients are screened
As can be seen from Table 3：DIFFERENT FEED additive has material impact to the lean meat percentage and back-fat thickness of Carnis Sus domestica.By
The feed additive that Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum are prepared so that Carnis Sus domestica
Back-fat thickness has reached 2.40cm, hence it is evident that less than feed additive prepared by other microbial inoculums；Lean meat percentage is 64.8%, significant high
In feed additive prepared by other microbial inoculums.Test by more than, filtering out can significantly reduce Carnis Sus domestica back-fat thickness, increase lean meat
The component of the feed additive of rate is：Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum.
Impact of the DIFFERENT FEED additive of table 3 to meat quality
The amounts of components screening experiment of the feed additive of experimental example 2
1st, test method
On the basis of experimental example 1, by Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, nisin bacteria agent three categories of etiologic factors
Plain three levels (table 4) prepare feed additive；Selection age in days is close to, body weight is close, be in a good state of health piglet 1120,
Designed using completely randomized experiment, be divided into 28 groups, 40 per group.The feed additive for preparing is added with 10% ratio
To in the basal diet of pig, above-mentioned piglet is fed respectively, one of which only feeds basal diet, treats feeding three as control
Month, butcher, calculate lean meat percentage and back-fat thickness.
Factor level table (the unit of table 4：kg)
2nd, result of the test
The lean meat percentage and back-fat thickness of pig is shown in Table 5, and as can be seen from the table, feed additive prepared by a2+b2+c2 makes
The back-fat thickness for obtaining Carnis Sus domestica has reached 2.21cm, hence it is evident that less than feed additive prepared by other dosage microbial inoculums；Lean meat percentage is
66.4%, hence it is evident that higher than feed additive prepared by other dosage microbial inoculums.Therefore, a2+b2+c2 (i.e. Bacillus pumilus are selected
12 parts of microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum) composition feed additive, after mixing with pig basal diet
Feeding pig can significantly improve the quality of Carnis Sus domestica, improve lean meat percentage.
Impact of the DIFFERENT FEED additive of table 5 to meat quality
The Chinese medicinal components screening experiment of the feed additive of experimental example 3
1st, test method
The preparation of 1.1 feed additives
Feed additive 1, according to following weight each component is weighed：Bacillus pumilus microbial inoculum 12kg, lactobacillus casei bacterium
Agent 7kg, enterococcus faecalis microbial inoculum 5kg；
Preparation method：Above-mentioned microbial inoculum is mixed to prepare into respectively microbial inoculum pre-mixing agent with adjuvant, wherein, adjuvant used be starch with
The mixture of oligosaccharide, microbial inoculum is 1 with the mass ratio of adjuvant:10, obtained pre-mixing agent is mixed, obtain final product.
Feed additive 2：Rhizoma Polygoni Cuspidati 8kg, Rhizoma Alismatis 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 3：Rhizoma Polygoni Cuspidati 8kg, Rhizoma Anemarrhenae 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 4：Rhizoma Polygoni Cuspidati 8kg, Pericarpium Citri Reticulatae 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 5：Rhizoma Polygoni Cuspidati 8kg, Cortex Cinnamomi 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 6：Rhizoma Polygoni Cuspidati 8kg, Radix Astragali 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 7：Rhizoma Alismatis 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 8：Rhizoma Anemarrhenae 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 9：Pericarpium Citri Reticulatae 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 10：Cortex Cinnamomi 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 11：Radix Astragali 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
Feed additive 12：Rhizoma Polygoni Cuspidati 8kg, Herba Erodii 4kg after addition is crushed on the basis of feed additive 1；
1.2 feeding method
Selection age in days is close to, body weight is close, be in a good state of health piglet 480, is designed using completely randomized experiment, is divided into
12 groups, 40 per group.The feed additive 1-12 for preparing is added in the basal diet of pig with 10% ratio, respectively
Above-mentioned piglet is fed, feeding three months is treated, is butchered, calculate lean meat percentage and back-fat thickness.
2nd, result of the test
The lean meat percentage and back-fat thickness of Carnis Sus domestica is shown in Table 6.
Impact of the DIFFERENT FEED additive of table 6 to meat quality
As can be seen from the table, by Bacillus pumilus microbial inoculum 12kg, lactobacillus casei microbial inoculum 7kg, enterococcus faecalis microbial inoculum
Feed additive 1 made by 5kg causes the back-fat thickness of Carnis Sus domestica to reach 2.21cm, and lean meat percentage has reached 66.4%.Short and small
On the basis of gemma bacillus agent 12kg, lactobacillus casei microbial inoculum 7kg, enterococcus faecalis microbial inoculum 5kg, addition Rhizoma Polygoni Cuspidati 8kg, Herba Erodii
The feed additive 12 of 4kg can effectively improve the quality of Carnis Sus domestica so that the back-fat thickness of Carnis Sus domestica has reached 1.94cm, adds than feedstuff
Plus agent 1 reduces 13.9%, and significantly lower than feed additive 2-11；Lean meat percentage has reached 78.9%, is significantly higher than feedstuff and adds
Plus agent 2-11.Feed additive 1-11 groups are not significantly different from back-fat thickness and lean meat percentage.Short and small spore described above
There is significant synergistic function between bacillus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum and Rhizoma Polygoni Cuspidati, Herba Erodii,
Its compatibility can significantly be increased the quality of Carnis Sus domestica together, the back-fat thickness of Carnis Sus domestica is reduced, lean meat percentage is improved.By contrast,
By Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum and enterococcus faecalis microbial inoculum together with other several drugses compatibilities after, it is right
In the quality for increasing Carnis Sus domestica, the back-fat thickness of Carnis Sus domestica is reduced, improve using for lean meat percentage not clear aobvious.
Claims (10)
1. it is a kind of improve lean meat percentage feed additive, it is characterised in that including following components：Bacillus pumilus
(Bacillus pumilus) microbial inoculum, lactobacillus casei (Lactobacillus casei) microbial inoculum and enterococcus faecalis
(Enterococcus faecium) microbial inoculum.
2. according to the feed additive described in claim 1, it is characterised in that the weight portion of each component is：Bacillus pumilus
Microbial inoculum 8-16 parts, lactobacillus casei microbial inoculum 5-9 parts, enterococcus faecalis microbial inoculum 3-7 parts；Preferably, the weight portion of each component is：It is short and small
12 parts of gemma bacillus agent, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum.
3. it is a kind of to promote growth of animals or poultry, the feed additive that enhances disease resistance, it is characterised in that including following components：Short and small bud
Spore bacillus microbial inoculum, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum, Rhizoma Polygoni Cuspidati and Herba Erodii.
4. according to the feed additive described in claim 3, it is characterised in that the weight portion of each component is：Bacillus pumilus
Microbial inoculum 8-16 parts, lactobacillus casei microbial inoculum 5-9 parts, enterococcus faecalis microbial inoculum 3-7 parts, Rhizoma Polygoni Cuspidati 4-12 parts, Herba Erodii 2-6 parts；It is preferred that
, the weight of each component is divided into 12 parts of Bacillus pumilus microbial inoculum, 7 parts of lactobacillus casei microbial inoculum, 5 parts of enterococcus faecalis microbial inoculum, tiger
8 parts of cane, 4 parts of Herba Erodii.
5. according to the feed additive described in claim 1-4, it is characterised in that：The viable bacteria content of each component is：It is short and small
Bacillus cereuss are 1.0 × 1011-3.0×1011cfu/g；Lactobacillus casei is 5.0 × 1011-7.0×1011Cfu/g, enterococcus faecalis
For 2.0 × 1011-3.0×1011cfu/g；Preferably：Bacillus pumilus are 2.0 × 1011cfu/g；Lactobacillus casei is
6.0×1011Cfu/g, enterococcus faecalis are 2.5 × 1011cfu/g。
6. the preparation method of the feed additive described in claim 1 or 2, it is characterised in that comprise the following steps：
(1) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum are carried out respectively with adjuvant being mixed with
To Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent；
(2) Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent are mixed,
Obtain final product.
7. the preparation method of the feed additive described in claim 3 or 4, it is characterised in that comprise the following steps：
(1) Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum, enterococcus faecalis microbial inoculum are carried out respectively with adjuvant being mixed with
To Bacillus pumilus microbial inoculum pre-mixing agent, lactobacillus casei microbial inoculum pre-mixing agent and enterococcus faecalis microbial inoculum pre-mixing agent；
(2) Rhizoma Polygoni Cuspidati, Herba Erodii are crushed into obtain Giant Knotweed Rhizome, Herba Erodii powder；
(3) by Bacillus pumilus microbial inoculum, lactobacillus casei microbial inoculum pre-mixing agent, enterococcus faecalis microbial inoculum pre-mixing agent, Giant Knotweed Rhizome, old
Stork grass meal end mixes, and obtains final product.
8. according to the preparation method described in claim 6 or 7, it is characterised in that：Described adjuvant is starch and oligosaccharide or fruit
The mixture of polysaccharide any one or two kinds.
9. application of the feed additive described in Claims 1-4 any one in domestic animal lean meat percentage is improved.
10. according to the application described in claim 9, it is characterised in that：The domestic animal, including pig, cattle, sheep or horse；Preferably
Pig.
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