CN106490305A - A kind of feed additive for reducing meat cholesterol level and its preparation method and application - Google Patents
A kind of feed additive for reducing meat cholesterol level and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of reduce feed additive of cholesterol level and its preparation method and application in poultry meat.The component of the feed additive includes：Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum.The present invention further discloses the preparation method of the feed additive, including：(1) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively, is prepared Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent；(2) above-mentioned microbial inoculum pre-mixing agent is mixed, is obtained final product.Do not contain antibiotic in feed additive of the present invention, preparation process is simple, extensively, low cost is economical and practical for raw material sources, noresidue, has no toxic side effect；The quality of meat can be effectively improved, reduces the cholesterol level in Carnis Sus domestica.
Description
Technical field
The present invention relates to a kind of feed additive for reducing meat cholesterol level, more particularly to reduce cholesterol in pork content
Feed additive, the invention further relates to the preparation method of the feed additive, belongs to the additive agent field of pig feed.
Background technology
Carnis Sus domestica is Chinese main carnivorous source, very popular, but present common cholesterol in pork is all
Comparison is high.The too high easy initiation kidney of cholesterol level, blood vessel, heart, osteoporosis diseases, therefore people are for gallbladder in Carnis Sus domestica
The requirement more and more higher of sterin content.In the cultivation of traditional pig, medicine the making in feedstuff such as antibiotic, hormone, preservative
Had certain effect with having to reduction cholesterol level, but the raising with public life level, pursue green, safe and healthy
Life idea oneself through being rooted in the hearts of the people, instantly, pollution-free food is just favored by increasing people with its exclusive glamour, raw
Producing green, safety, the food of drug residue free becomes the development trend of grocery trade, and the Carnis Sus domestica for producing health will also be foster
Grow the developing direction of industry.
At this stage, the use of probiotics and Chinese medicine is endangered because of safety non-toxic, without accumulation residual, without introduction potentially pathogenic organism,
Non-environmental-pollution, without Drug resistance the features such as, in widespread attention in aquaculture.Therefore, gallbladder in a kind of effectively reduction meat is developed
The Traditional Chinese medicine probiotic feed additive of sterin content, for the sound development of poultry significant.
Content of the invention
An object of the present invention is to provide the Traditional Chinese medicine probiotic feed additive that can reduce cholesterol level in poultry meat,
The feed additive has without antibiotic, and preparation process is simple, raw material sources are extensive, the advantage such as drug residue free.
The second object of the present invention is to provide the preparation method of the feed additive.
The third object of the present invention is answering in the livestock culture for reducing meat cholesterol level by the feed additive
With.
For reaching above-mentioned purpose, the technical solution adopted in the present invention is：
The invention discloses a kind of feed additive for reducing cholesterol level in poultry meat, including following components：Wax-like
Gemma bacillus agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum.
Preferably, the weight portion of each component is：Bacillus cercuses microbial inoculum 10-18 parts, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5-12 parts, rice
Aspergillosis microbial inoculum 3-7 parts；
It is furthermore preferred that the weight portion of each component is：14 parts of Bacillus cercuses microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae
5 parts of microbial inoculum.
Wherein, the viable bacteria content of each component is：Bacillus cercuses are 2.5 × 1010-3.5×1010Cfu/g, breast
Streptococcus are 1.5 × 1010-4.5×1010Cfu/g, aspergillus oryzae are 1.0 × 1010-3.0×1010cfu/g；Preferably wax-like bud
Spore bacillus is 3.0 × 1010Cfu/g, Streptococcus lactis (Lister) Lohnis 1909.554. are 2.5 × 1010Cfu/g, aspergillus oryzae are 2.0 × 1010cfu/g.
The present invention has carried out screening test to the component of feed additive.First by Bacillus cercuses microbial inoculum, condense bud
Spore bacillus microbial inoculum, bacillus subtilis microbial agent, bacillus licheniformis agent, four microbial inoculums of Bafillus natt microbial inoculum are respectively prepared
Feed additive, is added in basal diet with 8% ratio, after feeding three months, is butchered, and the cholesterol that surveys in Carnis Sus domestica contains
Amount, as a result shows the feed additive prepared by Bacillus cercuses microbial inoculum, and the cholesterol level of Carnis Sus domestica is 55.2mg/
100g, hence it is evident that less than the feed additive prepared without feed additive group and other microbial inoculums；Then just Bacillus cercuses bacterium
Agent together with Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, thermophilus bacteria agent, streptococcus faecalis microbial inoculum, nisin bacteria agent is made feedstuff respectively and is added
Plus agent, it is added in basal diet with 8% ratio, after feeding three months, is butchered, the cholesterol level that surveys in Carnis Sus domestica, as a result
Show that the feed additive prepared by Bacillus cercuses microbial inoculum and Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, the cholesterol level of Carnis Sus domestica are
50.6mg/100g, hence it is evident that less than the feed additive prepared without feed additive group and other microbial inoculums；Finally, by wax-like spore
Bacillus microbial inoculum and Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum make feed additive respectively together with aspergillus oryzae microbial inoculum, aspergillus niger microbial inoculum, with 8% ratio
Example is added in basal diet, after feeding three months, butchers, and as a result the cholesterol level that surveys in Carnis Sus domestica shows by wax-like spore
The feed additive that bacillus microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are made, the cholesterol level of Carnis Sus domestica is 44.3mg/
100g, hence it is evident that less than the feed additive prepared without feed additive group and other microbial inoculums, and without feed additive group and other
Difference between feed additive group without significance.Result above shows Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and rice
The feed additive that aspergillosis microbial inoculum is prepared can effectively lift the quality of Carnis Sus domestica, and the cholesterol that can significantly reduce Carnis Sus domestica contains
Amount.
On this basis, probiotic bacteria dosage determination tests of the present invention by Three factors-levels, it is determined that the feedstuff adds
Plus the optimum amount of agent each component：14 parts of Bacillus cercuses microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, 5 parts of aspergillus oryzae microbial inoculum.
Invention also discloses the preparation method of above-mentioned feed additive, including：(1) by Bacillus cercuses microbial inoculum, breast
Hammer bacteria agent and aspergillus oryzae microbial inoculum are carried out being mixed with adjuvant respectively and obtain Bacillus cercuses microbial inoculum pre-mixing agent, newborn hammer
Bacteria agent pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent；
(2) Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent are mixed,
Obtain final product；
Wherein, described adjuvant is the mixture of any one or two kinds of starch and oligosaccharide or levan；Microbial inoculum with auxiliary
The mass ratio of material is 1:5-15, preferably 1:10.
The present invention further discloses a kind of feed additive for reducing cholesterol level in poultry meat, including with the following group
Point：Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum, Semen Amaranthi tricoloris and Herba Emiliae.
Preferably, the weight portion of each component is：Bacillus cercuses microbial inoculum 10-18 parts, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5-12 parts, rice
Aspergillosis microbial inoculum 3-7 parts, Semen Amaranthi tricoloris 7-12 parts, Herba Emiliae 4-8 parts；
It is furthermore preferred that the weight of each component is divided into 14 parts of Bacillus cercuses microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae
5 parts of microbial inoculum, 9 parts of Semen Amaranthi tricoloris, 6 parts of Herba Emiliae；
Wherein, the viable bacteria content of each component is：Bacillus cercuses are 2.5 × 1010-3.5×1010Cfu/g, breast
Streptococcus are 1.5 × 1010-4.5×1010Cfu/g, aspergillus oryzae are 1.0 × 1010-3.0×1010cfu/g；Preferably wax-like bud
Spore bacillus is 3.0 × 1010Cfu/g, Streptococcus lactis (Lister) Lohnis 1909.554. are 2.5 × 1010Cfu/g, aspergillus oryzae are 2.0 × 1010cfu/g.
Seed of the Semen Amaranthi tricoloris for amaranthaceous plant Herba Amaranthi tricoloriss.Nature and flavor：Sweet, tremble with fear.Indication：Liver heat removing and eyesight improving, smoothening secretion.Control blue cataract
Barrier, mesh mist are not clear, hemotochyluria, difficulty in urination and defecation.
Herb or whole herb with root of the Herba Emiliae for feverfew Herba Duchesneae Indicae.Nature and flavor：Hardship, cool.Indication：Heat clearing away, diuretic,
Removing heat from blood, removing toxic substances.Control dysentery, suffer from diarrhoea, have blood in stool, edema, acute appendicitis engage ear, mesh also, throat moth, furuncle, toxic swelling.
The present invention is containing 14 parts of Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 8 for improving the effect of feed additive
Chinese medicinal components be with the addition of in part, the feed additive of 5 parts of aspergillus oryzae microbial inoculum.Specially：In 14 parts of Bacillus cercuses microbial inoculum, breast
On the basis of 8 parts of hammer bacteria agent, 5 parts of aspergillus oryzae microbial inoculum, add (1) Semen Amaranthi tricoloris 9kg, Poria 6kg respectively；(2) Semen Amaranthi tricoloris 9kg, Rhizoma Polygoni Cuspidati
6kg；(3) Semen Amaranthi tricoloris 9kg, Radix Astragali 6kg；(4) Semen Amaranthi tricoloris 9kg, Radix Paeoniae Alba 6kg；(5) Semen Amaranthi tricoloris 9kg, Pericarpium Citri Reticulatae 6kg；(6) Poria 9kg, Radix Rumicis Japonici
Careless 6kg；(7) Rhizoma Polygoni Cuspidati 9kg, Herba Emiliae 6kg；(8) Radix Astragali 9kg, Herba Emiliae 6kg；(9) Radix Paeoniae Alba 9kg, Herba Emiliae 6kg；(10) Pericarpium Citri Reticulatae
9kg, Herba Emiliae 6kg；(11) Semen Amaranthi tricoloris 9kg, Herba Emiliae 6kg, make feed additive.As a result show in Bacillus cercuses microbial inoculum
14 parts, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, add 9 parts of Semen Amaranthi tricoloris, 12 energy of feed additive of 6 parts of Herba Emiliae on the basis of 5 parts of aspergillus oryzae microbial inoculum
The content of cholesterol in Carnis Sus domestica is significantly reduced, 26.8mg/100g has been reached.Bacillus cercuses microbial inoculum described above, newborn hammer
There is significant synergistic function between bacteria agent, aspergillus oryzae microbial inoculum and Semen Amaranthi tricoloris, Herba Emiliae, its compatibility can be improved together
The quality of Carnis Sus domestica, significantly reduces the cholesterol level in Carnis Sus domestica.By contrast, by Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. bacterium
After agent, aspergillus oryzae microbial inoculum are together with other several drugses compatibilities, on Carnis Sus domestica in cholesterol level affect less.
The invention also discloses the preparation method of above-mentioned feed additive, including：
(1) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Obtain Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent；
(2) Semen Amaranthi tricoloris, Herba Emiliae are crushed to obtain Semen Amaranthi tricoloris powder, Herba Emiliae powder；
(3) by Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent, Semen Amaranthi tricoloris
Powder, Herba Emiliae powder are mixed, and are obtained final product.
Wherein, described adjuvant is the mixture of any one or two kinds of starch and oligosaccharide or levan.Described is auxiliary
Expect the mixture for any one or two kinds of starch and oligosaccharide or levan；Microbial inoculum is 1 with the mass ratio of adjuvant:5-15,
Preferably 1:10.
The application that the feed additive is further disclosed again in promoting poultry growth, enhancing disease resistance of the invention,
Wherein, the poultry, including chicken, duck, pig, cattle, sheep or horse；Preferably pig.
Microbial inoculum of the present invention carries out culture acquisition according to the microbial inoculum training method provided by commercial sources.
Technical solution of the present invention compared with prior art, has the advantages that：
Antibiotic is not contained in feed additive of the present invention, and preparation process is simple, raw material sources are extensive, low cost, economical real
With, noresidue, have no toxic side effect；The quality of meat can be effectively improved, reduces the cholesterol level in Carnis Sus domestica.
Specific embodiment
Further describe the present invention with reference to specific embodiment, advantages of the present invention and feature will be with description and
Apparent.It should be understood that the embodiment is only exemplary, the scope of the present invention is not construed as limiting.Art technology
Personnel should be understood that without departing from the spirit and scope of the invention can be to the details of technical solution of the present invention and form
Modify or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
1st, strain source
Bacillus cercuses microbial inoculum：ACCC03002 is purchased from Chinese agriculture Culture Collection；
Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum：ACCC11048 is purchased from Chinese agriculture Culture Collection；
Aspergillus oryzae microbial inoculum：CGMCC NO.3.4437 are purchased from China General Microbiological culture presevation administrative center.
Microbial inoculum used in the present invention is according to method (species of culture medium and amplification side given during purchase strain
Method) prepare (can be commercially available from commercial channels).
The preparation of 1 feed additive of embodiment
(1) each component is weighed：Bacillus cercuses microbial inoculum 10kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5kg, aspergillus oryzae microbial inoculum 3kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is starch and oligomerization
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:5；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of 2 feed additive of embodiment
(1) each component is weighed：Bacillus cercuses microbial inoculum 18kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 12kg, aspergillus oryzae microbial inoculum 7kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is that starch is poly- with fruit
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:15；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of 3 feed additive of embodiment
(1) each component is weighed：Bacillus cercuses microbial inoculum 14kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 8kg, aspergillus oryzae microbial inoculum 5kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is starch and oligomerization
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:10；
(3) by the microbial inoculum pre-mixing agent of above-mentioned preparation, mix homogeneously is obtained final product.
The preparation of 4 feed additive of embodiment；
(1) each component is weighed：Bacillus cercuses microbial inoculum 10kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5kg, aspergillus oryzae microbial inoculum 3kg, Semen Amaranthi tricoloris
7kg, Herba Emiliae 4kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is that starch is poly- with fruit
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:5；
(3) Semen Amaranthi tricoloris, Herba Emiliae are crushed, is obtained Semen Amaranthi tricoloris powder, Herba Emiliae powder；
(4) by the microbial inoculum pre-mixing agent and Semen Amaranthi tricoloris powder of above-mentioned preparation, Herba Emiliae powder, mix homogeneously is obtained final product.
The preparation of 5 feed additive of embodiment
(1) each component is weighed：Bacillus cercuses microbial inoculum 18kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 12kg, aspergillus oryzae microbial inoculum 7kg, Herba Amaranthi tricoloriss
Real 12kg, Herba Emiliae 8kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is that starch is poly- with fruit
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:15；
(3) Semen Amaranthi tricoloris, Herba Emiliae are crushed, is obtained Semen Amaranthi tricoloris powder, Herba Emiliae powder；
(4) by the microbial inoculum pre-mixing agent and Semen Amaranthi tricoloris powder of above-mentioned preparation, Herba Emiliae powder, mix homogeneously is obtained final product.
The preparation of 6 feed additive of embodiment
(1) each component is weighed：Bacillus cercuses microbial inoculum 14kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 8kg, aspergillus oryzae microbial inoculum 5kg, Semen Amaranthi tricoloris
9kg, Herba Emiliae 6kg；
(2) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are mixed with adjuvant respectively
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum pre-mixing agent is obtained, wherein, adjuvant used is that starch is poly- with fruit
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:10；
(3) Semen Amaranthi tricoloris, Herba Emiliae are crushed, is obtained Semen Amaranthi tricoloris powder, Herba Emiliae powder；
(4) by the microbial inoculum pre-mixing agent and Semen Amaranthi tricoloris powder of above-mentioned preparation, Herba Emiliae powder, mix homogeneously is obtained final product.
The probiotic composition screening experiment of 1 feed additive of experimental example
1st, experimental technique
1.1 one-components are screened
By (1) Bacillus cercuses microbial inoculum, (2) Bacillus coagulans microbial inoculum, (3) bacillus subtilis microbial agent, (4) lichens
Gemma bacillus agent, (5) Bafillus natt microbial inoculum prepare 5 kinds of feed additives, wherein, adjuvant used respectively with adjuvant
Mass ratio for starch and the mixture of oligosaccharide, microbial inoculum and adjuvant is 1:10.Selection age in days is close to, body weight is close, healthy
Piglet 240, are designed using completely randomized experiment, are divided into 6 groups in order, 40 per group.The feedstuff for preparing is added
Agent is added in the basal diet of pig with 8% ratio, feeds above-mentioned piglet respectively, and one of which only feeds basis as control
Daily ration, after feeding 3 months, butchers, and determines the cholesterol level in Carnis Sus domestica.
1.2 compounding ingredients screenings two-by-two
By Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, thermophilus bacteria agent, streptococcus faecalis microbial inoculum, nisin
Bacteria agent prepares microbial inoculum pre-mixing agent respectively with adjuvant, and wherein, adjuvant used is the mixture of starch and oligosaccharide, microbial inoculum with
The mass ratio of adjuvant is 1:10, then that Bacillus cercuses microbial inoculum pre-mixing agent is thermophilic with (1) Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, (2) respectively
Hot hammer bacteria agent, (3) streptococcus faecalis microbial inoculum, the pre-mixing agent of (4) nisin bacteria agent are with 1:1 ratio is mixed and made into feedstuff
Additive, selection age in days is close to, body weight is close, be in a good state of health piglet 200, is designed using completely randomized experiment, is divided into 5
Group, 40 per group.The feed additive for preparing is added in the basal diet of pig with 8% ratio, respectively in feeding
Piglet is stated, one of which only feeds basal diet as control, after feeding 3 months, butchers, and the cholesterol determined in Carnis Sus domestica contains
Amount.
1.3 33 compounding ingredients are screened
Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum, aspergillus niger microbial inoculum are prepared into adjuvant respectively
Microbial inoculum pre-mixing agent is arrived, wherein, adjuvant used is that the mass ratio of the mixture of starch and oligosaccharide, microbial inoculum and adjuvant is 1:10,
Then by Bacillus cercuses microbial inoculum pre-mixing agent and Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent respectively with (1) aspergillus oryzae microbial inoculum, (2) aspergillus niger
The pre-mixing agent of microbial inoculum is with 1:1:1 ratio is mixed and made into feed additive, and selection age in days is close to, body weight is close, health status are good
Good piglet 120, is designed using completely randomized experiment, is divided into 3 groups, 40 per group.By the feed additive for preparing with 8%
Ratio be added in the basal diet of pig, feed above-mentioned piglet respectively, one of which only feeds a basal diet as control, treats
After feeding 3 months, butcher, determine the cholesterol level in Carnis Sus domestica.
2nd, experimental result
2.1 one-components are screened
As can be seen from Table 1：DIFFERENT FEED additive has material impact to the cholesterol level of Carnis Sus domestica.By wax-like bud
The feed additive that spore bacillus microbial inoculum is prepared, the cholesterol level of Carnis Sus domestica are 55.2mg/100g, hence it is evident that less than adding without feedstuff
Plus feed additive prepared by agent group and other microbial inoculums.Bacillus cercuses microbial inoculum indicated above can significantly improve meat quality,
Reduce the cholesterol level of Carnis Sus domestica.
Impact of the 1 DIFFERENT FEED additive of table to cholesterol in pork content
2.2 compounding ingredients screenings two-by-two
As can be seen from Table 2：DIFFERENT FEED additive has material impact to the cholesterol level of Carnis Sus domestica.By wax-like bud
The feed additive that spore bacillus microbial inoculum and Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum are prepared, the cholesterol level of Carnis Sus domestica is 50.6mg/100g, bright
The aobvious feed additive less than without feed additive group and the preparation of other microbial inoculums.Bacillus cercuses microbial inoculum indicated above and newborn chain
Coccus microbial inoculum can significantly improve meat quality, reduce the cholesterol level of Carnis Sus domestica.
Impact of the 2 DIFFERENT FEED additive of table to cholesterol in pork content
1.3 33 compounding ingredients are screened
As can be seen from Table 3：DIFFERENT FEED additive has material impact to the cholesterol level of Carnis Sus domestica.By wax-like bud
The feed additive that spore bacillus microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are prepared, the cholesterol level of Carnis Sus domestica is
44.3mg/100g, hence it is evident that less than the feed additive prepared without feed additive group and other microbial inoculums.Wax-like spore indicated above
Bacillus microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum can significantly improve meat quality, reduce the cholesterol level of Carnis Sus domestica.
Impact of the 3 DIFFERENT FEED additive of table to cholesterol in pork content
The amounts of components screening experiment of 2 feed additive of experimental example
1st, test method
On the basis of experimental example 1, by Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum three categories of etiologic factors three water of element
Flat (table 4) prepares feed additive；Selection age in days is close to, body weight is close, be in a good state of health piglet 1120, using complete
Full random experiment design, is divided into 28 groups, 40 per group.The feed additive for preparing is added to pig with 8% ratio
In basal diet, above-mentioned piglet is fed respectively, one of which only feeds basal diet as control, after feeding 3 months, slaughter
Kill, determine the cholesterol level in Carnis Sus domestica.
4 factor level table (unit of table：kg)
2nd, result of the test
After feeding pig three months with above feed additive, in Carnis Sus domestica, the content of cholesterol is shown in Table 5, can from table
Go out, the cholesterol level of a2+b2+c2 pigs is minimum, and significantly lower than other combinations, therefore, select a2+b2+c2 (i.e. wax-like spore
14 parts of bacillus microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, 5 parts of aspergillus oryzae microbial inoculum) composition feed additive, after mixing with pig basal diet
Feeding pig causes the quality of Carnis Sus domestica to be improved, and cholesterol level substantially reduces.
Impact of the 5 DIFFERENT FEED additive of table to cholesterol in pork content
The Chinese medicinal components screening experiment of 3 feed additive of experimental example
1st, test method
The preparation of 1.1 feed additives
Feed additive 1, weighs each component according to following weight：Bacillus cercuses microbial inoculum 14kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum
8kg, aspergillus oryzae microbial inoculum 5kg；
Preparation method：Above-mentioned microbial inoculum is mixed to prepare with adjuvant pre-mixing agent respectively, wherein, adjuvant used is starch and oligomerization
The mixture of sugar, microbial inoculum are 1 with the mass ratio of adjuvant:10, obtained pre-mixing agent is mixed, is obtained final product.
Feed additive 2：Add Semen Amaranthi tricoloris 9kg, Poria 6kg after crushing on the basis of feed additive 1；
Feed additive 3：Add Semen Amaranthi tricoloris 9kg, Rhizoma Polygoni Cuspidati 6kg after crushing on the basis of feed additive 1；
Feed additive 4：Add Semen Amaranthi tricoloris 9kg, Radix Astragali 6kg after crushing on the basis of feed additive 1；
Feed additive 5：Add Semen Amaranthi tricoloris 9kg, Radix Paeoniae Alba 6kg after crushing on the basis of feed additive 1；
Feed additive 6：Add Semen Amaranthi tricoloris 9kg, Pericarpium Citri Reticulatae 6kg after crushing on the basis of feed additive 1；
Feed additive 7：Add Poria 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1；
Feed additive 8：Add Rhizoma Polygoni Cuspidati 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1；
Feed additive 9：Add Radix Astragali 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1；
Feed additive 10：Add Radix Paeoniae Alba 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1；
Feed additive 11：Add Pericarpium Citri Reticulatae 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1；
Feed additive 12：Add Semen Amaranthi tricoloris 9kg, Herba Emiliae 6kg after crushing on the basis of feed additive 1.
1.2 feeding method
Selection age in days is close to, body weight is close, be in a good state of health piglet 480, is designed using completely randomized experiment, is divided into
12 groups, 40 per group.The feed additive 1-12 for preparing is added in the basal diet of pig with 8% ratio, respectively
Above-mentioned piglet is fed, after feeding 3 months, is butchered, determine the cholesterol level in Carnis Sus domestica.
2nd, result of the test
In Carnis Sus domestica, cholesterol level is shown in Table 6.
Impact of the 6 DIFFERENT FEED additive of table to pig growth performance
As can be seen from the table, by Bacillus cercuses microbial inoculum 14kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 8kg, aspergillus oryzae microbial inoculum 5kg systems
Into the feeding pig three months of feed additive 1 after, the cholesterol level of Carnis Sus domestica is 40.1mg/100g.In Bacillus cercuses bacterium
On the basis of agent 14kg, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 8kg, aspergillus oryzae microbial inoculum 5kg, add Semen Amaranthi tricoloris 9kg, the feed additive of Herba Emiliae 6kg
12 contents that can significantly reduce cholesterol in Carnis Sus domestica, have reached 26.8mg/100g.There was no significant difference for other each groups.Say above
Bright Bacillus cercuses microbial inoculum, between Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum and Semen Amaranthi tricoloris, Herba Emiliae have significant Synergistic
Effect, its compatibility can be improved the quality of Carnis Sus domestica together, the cholesterol level in Carnis Sus domestica is significantly reduced.By contrast, by wax
After shape gemma bacillus agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum are together with other several drugses compatibilities, to Carnis Sus domestica in gallbladder
Sterin content affects less.
Claims (10)
1. a kind of reduce poultry meat in cholesterol level feed additive, it is characterised in that including following each component：Wax-like bud
Spore bacillus (Bacillus cereus) microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. (Lactobacillus helveticus) microbial inoculum and aspergillus oryzae
(Aspergillus oryzae) microbial inoculum.
2. according to the feed additive described in claim 1, it is characterised in that the weight portion of each component is：Bacillus cercuses
Microbial inoculum 10-18 parts, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5-12 parts, aspergillus oryzae microbial inoculum 3-7 parts；Preferably, the weight portion of each component is：Wax-like bud
14 parts of spore bacillus microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, 5 parts of aspergillus oryzae microbial inoculum.
3. a kind of reduce poultry meat in cholesterol level feed additive, it is characterised in that including following components：Wax-like spore
Bacillus microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, aspergillus oryzae microbial inoculum, Semen Amaranthi tricoloris and Herba Emiliae.
4. according to the feed additive described in claim 3, it is characterised in that the weight portion of each component is：Bacillus cercuses
Microbial inoculum 10-18 parts, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum 5-12 parts, aspergillus oryzae microbial inoculum 3-7 parts, Semen Amaranthi tricoloris 7-12 parts, Herba Emiliae 4-8 parts；Preferably,
The weight of each component is divided into 14 parts of Bacillus cercuses microbial inoculum, 8 parts of Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum, 5 parts of aspergillus oryzae microbial inoculum, 9 parts of Semen Amaranthi tricoloris, sheep
6 parts of hoof grass.
5. according to the feed additive described in claim 1-4, it is characterised in that：The viable bacteria content of each component is：Wax-like
Bacillus cereuss are 2.5 × 1010-3.5×1010Cfu/g, Streptococcus lactis (Lister) Lohnis 1909.554. are 1.5 × 1010-4.5×1010Cfu/g, aspergillus oryzae are
1.0×1010-3.0×1010cfu/g；Preferably：Bacillus cercuses are 3.0 × 1010Cfu/g, Streptococcus lactis (Lister) Lohnis 1909.554. be 2.5 ×
1010Cfu/g, aspergillus oryzae are 2.0 × 1010cfu/g.
6. the preparation method of the feed additive described in claim 1 or 2, it is characterised in that comprise the following steps：
(1) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are carried out being mixed with obtaining with adjuvant respectively
Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent；
(2) Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent are mixed, i.e.,
?.
7. the preparation method of the feed additive described in claim 3 or 4, it is characterised in that comprise the following steps：
(1) Bacillus cercuses microbial inoculum, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum and aspergillus oryzae microbial inoculum are carried out being mixed with obtaining with adjuvant respectively
Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent；
(2) Semen Amaranthi tricoloris, Herba Emiliae are crushed to obtain Semen Amaranthi tricoloris powder, Herba Emiliae powder；
(3) by Bacillus cercuses microbial inoculum pre-mixing agent, Streptococcus lactis (Lister) Lohnis 1909.554. microbial inoculum pre-mixing agent and aspergillus oryzae microbial inoculum pre-mixing agent, Semen Amaranthi tricoloris powder
End, Herba Emiliae powder are mixed, and are obtained final product.
8. according to the preparation method described in claim 6 or 7, it is characterised in that：Described adjuvant is starch and oligosaccharide or fruit
The mixture of polysaccharide any one or two kinds.
9. the application of the cholesterol level in poultry meat is reduced of feed additive described in Claims 1-4 any one.
10. according to the application described in claim 9, it is characterised in that：The poultry, including chicken, duck, pig, cattle, sheep or horse；Excellent
Choosing for pig.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760232A (en) * | 2021-01-29 | 2021-05-07 | 江西科技师范大学 | Method for degrading cholesterol by using aspergillus oryzae and application |
| CN112877219A (en) * | 2021-01-29 | 2021-06-01 | 江西科技师范大学 | High-concentration cholesterol culture medium and preparation method and application thereof |
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|---|---|---|---|---|
| CN105154372A (en) * | 2015-09-30 | 2015-12-16 | 胡小勇 | Compound microbe preparation and poultry fermenting bed padding |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154372A (en) * | 2015-09-30 | 2015-12-16 | 胡小勇 | Compound microbe preparation and poultry fermenting bed padding |
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| 肖莹莹等: "肉食动物肠道中芽孢杆菌的分离和降胆固醇能力的测定", 《黑龙江畜牧兽医》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760232A (en) * | 2021-01-29 | 2021-05-07 | 江西科技师范大学 | Method for degrading cholesterol by using aspergillus oryzae and application |
| CN112877219A (en) * | 2021-01-29 | 2021-06-01 | 江西科技师范大学 | High-concentration cholesterol culture medium and preparation method and application thereof |
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