CN103549115A - Complex microbial inoculant for livestock breeding - Google Patents
Complex microbial inoculant for livestock breeding Download PDFInfo
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- CN103549115A CN103549115A CN201310514348.8A CN201310514348A CN103549115A CN 103549115 A CN103549115 A CN 103549115A CN 201310514348 A CN201310514348 A CN 201310514348A CN 103549115 A CN103549115 A CN 103549115A
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- cfu
- viable count
- composite bacteria
- agent capable
- bacteria agent
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Abstract
The invention aims at providing a complex microbial inoculant for livestock breeding. The complex microbial inoculant comprises bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus, clostridium butyricum, a carrier and a protective agent. According to the complex microbial inoculant, by using a synergistic effect among probiotics, the immunity of bred animals can be effectively improved, the disease resistance of the animals is enhanced, the digestibility of livestock to a feed can be improved, the feed-to-meat ratio is reduced, the interest rate return of feed is increased, the economic benefit is increased for a farmer, and a new environment-friendly breeding mode is realized.
Description
Technical field
The present invention relates to microbe application field, be specifically related to a kind of livestock and poultry cultivation composite bacteria agent capable.
Background technology
China has become world's Production of Livestock and Poultry and big trading nation now, wherein egg production scale leaps to the first in the world, poultry prod output occupies the second in the world, but aquaculture faces a major issue at present, the use of numerous exactly auxins and medicine class feed addictive causes high amount of drug residual, thereby cause fowl neat of animal kind to decline, bacterial drug resistance strengthens, and affects directly or indirectly the mankind's health.
In recent years, probiotic composition has become focus as green feed additive, and probio forms advantage profitable strain by the biological competition repulsive interaction in animal alimentary canal, carrys out the generation of prevention and control disease by strengthening animal body immunity, promote growth of animal, improve feed coefficient.And probio has good palatability conventionally, with certain sour-sweet sense, also can increase the feed intake of animal.Probiotics preparation is many with its function, have no side effect, noresidue, the advantage such as free from environmental pollution, replaces antibiotic as feed addictive, to cause the extensive concern of Chinese scholars and feedstuff industry.But in actual suitability for industrialized production, viable count is low, thalline effect is single, poor stability again strong restriction the scale of probiotic composition, industrialized development.
Summary of the invention
The present invention, for solving prior art problem, provides composite bacteria agent capable and preparation method thereof for a kind of livestock and poultry cultivation.Described composite bacteria agent capable can effectively regulate cultivated animals intestinal environment by the synergy of multiple probio, improves the immunity of cultivated animals, thereby reduces antibiotic use, realizes green cultivation.
One aspect of the present invention provides a kind of composite bacteria agent capable, bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum, carrier and protective agent, consists of.
In described composite bacteria agent capable, the viable count of bacillus subtilis is 10
8-10
9cfu/g.
In described composite bacteria agent capable, the viable count of bafillus natto is 10
9-10
10cfu/g.
In described composite bacteria agent capable, the viable count of bacillus coagulans is 10
7-10
8cfu/g.
In described composite bacteria agent capable, the viable count of Lactobacillus rhamnosus is 10
7-10
8cfu/g.
In described composite bacteria agent capable, the viable count of clostridium butyricum is 10
5-10
6cfu/g.
Described carrier is oyster shell whiting and cornstarch, and its mass ratio is 1:2-3.
Described protective agent is trehalose and sodium glutamate, and its mass ratio is 1:1.5-2.
In above-mentioned composite bacteria agent capable, bacterium powder and carrier and protectant mass ratio are 4:1-1.5:0.5-1.
The present invention provides the application of above-mentioned composite bacteria agent capable in livestock and poultry cultivation on the other hand.
The present invention also provides a kind of method of preparing above-mentioned composite bacterium powder preparation, specifically comprises the steps:
1) single strain fermentation: bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum five strain bacterium are fermented respectively, obtain zymocyte liquid;
2) high concentration single strain powder preparation: after above-mentioned each single strain fermentation ends, add carrier in zymotic fluid, carry out plate compression, obtain bacterium mud; 3～10% add above-mentioned protective agent in mass ratio, after emulsification evenly, by freeze drying or spray drying process, are prepared into high-concentration bacterial powder;
3) the bacterium powder of five kinds of bacterium that prepare is mixed by effective bacterium number, the viable count that the viable count in final bacterium powder is bacillus subtilis is 10
8-10
9the viable count of cfu/g, bafillus natto is 10
9-10
10the viable count of cfu/g, bacillus coagulans is 10
7-10
8the viable count of cfu/g, Lactobacillus rhamnosus is 10
7-10
8the viable count of cfu/g, clostridium butyricum is 10
5-10
6cfu/g.
Composite bacteria agent capable provided by the invention utilizes five kinds of synergies between probio, can effectively improve the immunity of cultivated animals, strengthen the resistance against diseases of animal, and can improve the digestibility of fowl poultry to feed, reduce feedstuff-meat ratio, improve price of deed rate, for raiser increases economic benefit, realize green, environment-protecting cultivation new model.The procedure of preparing composite bacteria agent capable provided by the invention is simple; easy to operate; and the carrier adopting in preparation process and protective agent are all comestible compositions; can guarantee the safety of microbial inoculum; the method can guarantee five kinds of rational proportions between bacterium; thereby realize the strongest collaborative facilitation, be suitable for large-scale promotion application.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that after having read content of the present invention, those skilled in the art can make various changes or modification to the present invention, and these equivalent form of values fall into the application's appended claims limited range equally.
Various reagent and the equipment that the present invention relates to, all can be selected from commercially available any one; The present invention relates to bacillus subtilis in bacterial strain (purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.3376); Bacillus coagulans (purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.10302); Bafillus natto (purchased from Chinese agriculture microorganism fungus kind preservation administrative center, deposit number ATCC10619); Lactobacillus rhamnosus (purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.577); Clostridium butyricum (purchased from Chinese common micro-organisms preservation administrative center, deposit number 1.335).
embodiment 1
, by bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum, carrier and protective agent, formed.Wherein the viable count of bacillus subtilis is 10
8cfu/g; The viable count of bafillus natto is 10
9cfu/g; The viable count of bacillus coagulans is 10
8cfu/g; The viable count of Lactobacillus rhamnosus is 10
8cfu/g; The viable count of clostridium butyricum is 10
5cfu/g.
Described carrier is oyster shell whiting and cornstarch, and its mass ratio is 1:2.
Described protective agent is trehalose and sodium glutamate, and its mass ratio is 1:1.5.
In above-mentioned composite bacteria agent capable, bacterium powder and carrier and protectant mass ratio are 4:1:0.5.
The method of above-mentioned composite bacterium powder preparation, specifically comprises the steps:
1) single strain fermentation: bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum five strain bacterium are fermented respectively, obtain zymocyte liquid;
2) high concentration single strain powder preparation: after above-mentioned each single strain fermentation ends, add carrier in zymotic fluid, carry out plate compression, obtain bacterium mud; 3% adds above-mentioned protective agent in mass ratio, after emulsification evenly, by freeze-drying method, is prepared into high-concentration bacterial powder;
3) the bacterium powder of five kinds of bacterium that prepare is mixed by effective bacterium number, the viable count that the viable count in final bacterium powder is bacillus subtilis is 10
8cfu/g; The viable count of bafillus natto is 10
9cfu/g; The viable count of bacillus coagulans is 10
8cfu/g; The viable count of Lactobacillus rhamnosus is 10
8cfu/g; The viable count of clostridium butyricum is 10
5cfu/g.
embodiment 2
, by bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum, carrier and protective agent, formed.Wherein the viable count of bacillus subtilis is 10
9cfu/g; The viable count of bafillus natto is 10
10cfu/g; The viable count of bacillus coagulans is 10
7cfu/g; The viable count of Lactobacillus rhamnosus is 10
7cfu/g; The viable count of clostridium butyricum is 10
6cfu/g.
Described carrier is oyster shell whiting and cornstarch, and its mass ratio is 1:3.
Described protective agent is trehalose and sodium glutamate, and its mass ratio is 1:2.
In above-mentioned composite bacteria agent capable, bacterium powder and carrier and protectant mass ratio are 4:1.5:1.
The method of above-mentioned composite bacterium powder preparation, specifically comprises the steps:
1) single strain fermentation: bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum five strain bacterium are fermented respectively, obtain zymocyte liquid;
2) high concentration single strain powder preparation: after above-mentioned each single strain fermentation ends, add carrier in zymotic fluid, carry out plate compression, obtain bacterium mud; 10% adds above-mentioned protective agent in mass ratio, after emulsification evenly, by spray drying process, is prepared into high-concentration bacterial powder;
3) the bacterium powder of five kinds of bacterium that prepare is mixed by effective bacterium number, the viable count that the viable count in final bacterium powder is bacillus subtilis is 10
9cfu/g; The viable count of bafillus natto is 10
10cfu/g; The viable count of bacillus coagulans is 10
7cfu/g; The viable count of Lactobacillus rhamnosus is 10
7cfu/g; The viable count of clostridium butyricum is 10
6cfu/g.
embodiment 3
, by bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum, carrier and protective agent, formed.Wherein the viable count of bacillus subtilis is 3 * 10
8cfu/g; The viable count of bafillus natto is 6 * 10
9cfu/g; The viable count of bacillus coagulans is 5 * 10
7cfu/g; The viable count of Lactobacillus rhamnosus is 8 * 10
7cfu/g; The viable count of clostridium butyricum is 3 * 10
5cfu/g.
Described carrier is oyster shell whiting and cornstarch, and its mass ratio is 1:2.4.
Described protective agent is trehalose and sodium glutamate, and its mass ratio is 1:1.8.
In above-mentioned composite bacteria agent capable, bacterium powder and carrier and protectant mass ratio are 4:1.2:0.6.
The method of above-mentioned composite bacterium powder preparation, specifically comprises the steps:
1) single strain fermentation: bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum five strain bacterium are fermented respectively, obtain zymocyte liquid;
2) high concentration single strain powder preparation: after above-mentioned each single strain fermentation ends, add carrier in zymotic fluid, carry out plate compression, obtain bacterium mud; 8% adds above-mentioned protective agent in mass ratio, after emulsification evenly, by freeze drying or spray drying process, is prepared into high-concentration bacterial powder;
3) the bacterium powder of five kinds of bacterium that prepare is mixed by effective bacterium number, the viable count that the viable count in final bacterium powder is bacillus subtilis is 3 * 10
8cfu/g; The viable count of bafillus natto is 6 * 10
9cfu/g; The viable count of bacillus coagulans is 5 * 10
7cfu/g; The viable count of Lactobacillus rhamnosus is 8 * 10
7cfu/g; The viable count of clostridium butyricum is 3 * 10
5cfu/g.
the impact of embodiment 4 composite bacteria agent capables on meat chicken production performance
Purchase the white plumage meat cock of 1 age in days Roche 308, be divided into control group and experimental group, 20 repetitions of each processed group, each repeats 20 plumage chickens, the pilot production phase is 35 days, and wherein control group is the general goods daily ration of feeding, and experimental group is to feed to add the general goods daily ration of composite bacteria agent capable of the present invention, the situation of change of measuring feed intake in process of the test, material anharmonic ratio, body weight, experimental result is as shown in the table:
Meat chicken production performance statistics
| Group | Daily gain (g) | Feedstuff-meat ratio | Fatten index |
| Control group | 61.21±3.56 | 1.63±0.18b | 329.02±37.69 |
| Experimental group | 61.60±3.80 | 1.55±0.11a | 339.37±39.85 |
Result of the test in table is known, and experimental group broiler chicken feedstuff-meat ratio reduces by 0.08 than control group, with contrast difference remarkable (P < 0.05).In test index, experimental group daily gain, feedstuff-meat ratio, fatten index and be all better than control group.Result of the test shows, adds after composite bacteria agent capable of the present invention in feed, can greatly improve the production performance of broiler chicken, has not only reduced the production cost of broiler chicken, has also improved the production efficiency of broiler chicken, has increased raiser's economic benefit.
Claims (10)
1. a composite bacteria agent capable, is comprised of bacillus subtilis, bafillus natto, bacillus coagulans, Lactobacillus rhamnosus, clostridium butyricum, carrier and protective agent.
2. composite bacteria agent capable as claimed in claim 1, is characterized in that, the viable count of bacillus subtilis is 10
8-10
9cfu/g.
3. composite bacteria agent capable as claimed in claim 1, is characterized in that, the viable count of bafillus natto is 10
9-10
10cfu/g.
4. composite bacteria agent capable as claimed in claim 1, is characterized in that, the viable count of bacillus coagulans is 10
7-10
8cfu/g.
5. composite bacteria agent capable as claimed in claim 1, is characterized in that, the viable count of Lactobacillus rhamnosus is 10
7-10
8cfu/g.
6. composite bacteria agent capable as claimed in claim 1, is characterized in that, the viable count of clostridium butyricum is 10
5-10
6cfu/g.
7. composite bacteria agent capable as claimed in claim 1, is characterized in that, carrier is oyster shell whiting and cornstarch, and its mass ratio is 1:2-3.
8. composite bacteria agent capable as claimed in claim 1, is characterized in that, protective agent is trehalose and sodium glutamate, and its mass ratio is 1:1.5-2.
9. composite bacteria agent capable as claimed in claim 1, is characterized in that, bacterium powder and carrier and protectant mass ratio are 4:1-1.5:0.5-1.
10. the application of composite bacteria agent capable in livestock and poultry cultivation described in above-mentioned arbitrary claim.
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|---|---|---|---|
| CN201310514348.8A CN103549115A (en) | 2013-10-28 | 2013-10-28 | Complex microbial inoculant for livestock breeding |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310514348.8A CN103549115A (en) | 2013-10-28 | 2013-10-28 | Complex microbial inoculant for livestock breeding |
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| Publication Number | Publication Date |
|---|---|
| CN103549115A true CN103549115A (en) | 2014-02-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310514348.8A Pending CN103549115A (en) | 2013-10-28 | 2013-10-28 | Complex microbial inoculant for livestock breeding |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053524A (en) * | 2015-07-14 | 2015-11-18 | 湖南农业大学 | Rapeseed dreg microbial fermentation feed and preparation method and application thereof |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
| CN107427028A (en) * | 2015-04-06 | 2017-12-01 | 朝日可尔必思健康株式会社 | The feed conversion rate ameliorative way and method for breeding of poultry |
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| CN101461453A (en) * | 2009-01-15 | 2009-06-24 | 润盈生物工程（上海）有限公司 | Composite microbial feed additive for beasts and birds and production method |
| CN102511662A (en) * | 2012-01-05 | 2012-06-27 | 上海创博生态工程有限公司 | Composite probiotic microorganism feed additive, preparation method and application thereof |
-
2013
- 2013-10-28 CN CN201310514348.8A patent/CN103549115A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461453A (en) * | 2009-01-15 | 2009-06-24 | 润盈生物工程（上海）有限公司 | Composite microbial feed additive for beasts and birds and production method |
| CN102511662A (en) * | 2012-01-05 | 2012-06-27 | 上海创博生态工程有限公司 | Composite probiotic microorganism feed additive, preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 洪坚平 等: "《应用微生物学》", 30 September 2008 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107427028A (en) * | 2015-04-06 | 2017-12-01 | 朝日可尔必思健康株式会社 | The feed conversion rate ameliorative way and method for breeding of poultry |
| CN105053524A (en) * | 2015-07-14 | 2015-11-18 | 湖南农业大学 | Rapeseed dreg microbial fermentation feed and preparation method and application thereof |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
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Application publication date: 20140205 |