CN111838411A - Antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive and preparation method thereof - Google Patents
Antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive, which comprises Chinese herbal medicine fermented raw materials formed by fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea; the mass ratio of fructus forsythiae to radix isatidis to rhizoma atractylodis macrocephalae to fructus aurantii immaturus to fructus crataegi to echinacea purpurea is 2-3:6-10:4-7:4-9:20-26: 16-19; adding mixed fermentation strains into the Chinese herbal medicine fermentation raw materials; the mixed fermentation strain comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus. According to the invention, the Chinese herbal medicine with antibacterial and anti-inflammatory effects is fermented, so that the effective components in the Chinese herbal medicine are fully released, the synergistic effect of the Chinese herbal medicine and the animal intestinal probiotics is fully exerted, the harm of pathogenic bacteria to livestock and poultry is reduced, the survival rate of the livestock and poultry is greatly improved, the use amount of antibiotics is effectively reduced, the use amount is small, and the use cost is reduced.
Description
Technical Field
The invention relates to the field of feed additives, in particular to an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive and a preparation method thereof.
Background
In the breeding industry, most of the livestock and poultry are bred at high density, and the livestock and poultry are easy to get ill, so people often add various antibiotics into the feed to ensure the healthy growth of the animals, improve the production efficiency of the animals and prevent various diseases of the animals. However, too much intake of antibiotics can not only remain in livestock and poultry bodies to cause antibiotic residues in animal products to affect the physical health of consumers, but also the excrement discharged by the antibiotics also contains various antibiotics to cause antibiotic pollution in the environment. Therefore, people mostly adopt Chinese herbal medicine fermented feed to mix with a small amount of antibiotics, so that the use amount of the antibiotics is reduced while the livestock and poultry are prevented from getting ill, and the pollution is reduced. However, when the existing Chinese herbal medicine fermented feed is mixed with common feed, the traditional Chinese herbal medicine fermented feed needs to be larger in 5% -15% and the like to have obvious antibacterial effect, so that the cost is higher.
Disclosure of Invention
The invention discloses an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive and a preparation method thereof.
The purpose of the invention is realized by the following technical scheme:
an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive comprises Chinese herbal medicine fermented raw materials formed by fructus forsythiae, radix Isatidis, Atractylodis rhizoma, fructus Aurantii Immaturus, fructus crataegi and purple coneflower; the mass ratio of fructus forsythiae to radix isatidis to rhizoma atractylodis macrocephalae to fructus aurantii immaturus to fructus crataegi to echinacea purpurea is 2-3:6-10:4-7:4-9:20-26: 16-19; adding mixed fermentation strains into the Chinese herbal medicine fermentation raw materials; the mixed fermentation strain comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus.
Further improvement, the mass ratio of the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the rhamnose lactobacillus in the mixed fermentation strain in the Chinese herbal medicine fermentation raw materials is respectively 0.2-0.3%, 0.5-0.8%, 1-1.3% and 2-2.5%.
In a further improvement, the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the rhamnose lactobacillus are all cultured in a liquid culture medium to a logarithmic phase, then the bacterial liquid is inoculated into the Chinese herbal medicine fermentation raw materials according to the inoculation amount, and the mixture is uniformly stirred.
In a further improvement, the culture medium of the bacillus subtilis, the bacillus amyloliquefaciens and the bacillus belgii is as follows: 6g/L of yeast powder, 9g/L of trypsin vein, 9g/L of sodium chloride, 10g/L of CMC-Na10g, and the pH value of 6 +/-0.6; the culture medium of the rhamnose lactobacillus is 10g/L beef extract, 10g/L peptone, 10g/L yeast extract, 20g/L tomato juice, 10/L glucose, 0.5ml/L Tween, 17g/L calcium carbonate and 0.1g/L bromocresol green.
In a further improvement, the mass ratio of the largehead atractylodes rhizome to the immature bitter orange to the hawthorn to the echinacea is 2.5:7:5:6:22: 18.
A preparation method of an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive comprises the following steps:
weighing dried fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea, mixing and crushing the fructus forsythiae, the radix isatidis, the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea purpurea according to the mass ratio of 2-3:6-10:4-7:4-9:20-26:16-19, and then putting the mixture into a fermentation tank; adding a bacterial liquid of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus cultured to a logarithmic phase into a fermentation tank, wherein the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the lactobacillus rhamnosus are 0.2-0.3%, 0.5-0.8%, 1-1.3% and 2-2.5% respectively;
and step four, controlling the fermentation temperature to be 35 ℃, controlling the pH to be 4.0, fermenting for 2-3 days, and drying at 35-45 ℃ to obtain the Chinese herbal medicine fermented feed additive.
In a further improvement, the Chinese herbal medicine fermented feed additive is mixed with a common feed for use; the mass ratio of the Chinese herbal medicine fermented feed additive to the common feed is 0.1-0.2% and 99.8-99.9%.
The invention has the advantages that:
the invention discloses a medicament for resisting bacteria and diminishing inflammation, which comprises the following components: fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea are fermented, the cell walls of the Chinese herbal medicines are damaged through the strong degradation action of bacillus subtilis, bacillus amyloliquefaciens and bacillus belief chinensis, so that the effective components in the Chinese herbal medicines are fully released, the bacillus can secrete various digestive enzymes such as protease and cellulase and generate various metabolites such as animal growth promoting factors in the growth and reproduction process, and meanwhile, the effects of reducing toxic components in the Chinese herbal medicines, performing structural modification on certain medicinal components in the Chinese herbal medicines and the like are achieved, so that the medicinal effect of the Chinese herbal medicines is increased, the using amount of the Chinese herbal medicines is reduced, and the use of antibiotics is reduced or replaced. The addition of the rhamnose lactobacillus can reduce the pH value of the Chinese herbal medicine fermentation material, thereby improving the palatability of the fermented Chinese herbal medicine.
The bacillus subtilis is preferably the bacillus subtilis which is preserved in the China center for culture collection of microorganisms, the institute of microbiology of China academy of sciences No. 3 of West Lu No.1 of North Chen of the Korean region in Beijing, and the culture collection number of the bacillus subtilis is CGMCC NO.14220 or ACCC 01873. The Bacillus belgii preferably has preservation number of M2018841 or CGMCC 1.923. The preferred preservation number of the bacillus amyloliquefaciens is ACCC04304 or CGMCC 1.7463. The preferable preservation number of the rhamnose lactobacillus is CGMCC1.549 or ACCC05450
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below.
Detailed Description
Example 1
A preparation method of an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive comprises the following steps:
weighing dried fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea, mixing and crushing the fructus forsythiae, the radix isatidis, the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea purpurea according to the mass ratio of 2:6:7:9:20:19, and then putting the mixture into a fermentation tank;
adding a bacterial liquid of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus cultured to a logarithmic phase into a fermentation tank, wherein the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the lactobacillus rhamnosus are 0.3%, 0.5%, 1.3% and 2% respectively;
and step four, controlling the fermentation temperature to be 35 ℃, controlling the pH to be 4.0, and drying at 35 ℃ after fermenting for 2 days to obtain the Chinese herbal medicine fermented feed additive.
The Chinese herbal medicine fermented feed additive is mixed with common feed for use; the mass ratio of the Chinese herbal medicine fermented feed additive to the common feed is 0.15 percent to 99.85 percent.
Example 2
A preparation method of an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive comprises the following steps:
weighing dried fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea, mixing the fructus forsythiae, the radix isatidis, the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea purpurea according to the mass ratio of 3:10:4: 4-26: 19, crushing, and then putting into a fermentation tank;
adding a bacterial liquid of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus cultured to a logarithmic phase into a fermentation tank, wherein the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the lactobacillus rhamnosus are 0.2-%, 0.8%, 1% and 2.5% respectively;
and step four, controlling the fermentation temperature to be 35 ℃, controlling the pH to be 4.0, fermenting for 3 days, and drying at 45 ℃ to obtain the Chinese herbal medicine fermented feed additive.
The Chinese herbal medicine fermented feed additive is mixed with common feed for use; the mass ratio of the Chinese herbal medicine fermented feed additive to the common feed is 0.1% to 99.9%.
Example 3
A preparation method of an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive comprises the following steps:
weighing dried fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea, mixing the fructus forsythiae, the radix isatidis, the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea purpurea according to the mass ratio of 2.5:7:5:6:22:18, crushing, and then putting into a fermentation tank;
adding a bacterial liquid of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus cultured to a logarithmic phase into a fermentation tank, wherein the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the lactobacillus rhamnosus are 0.25%, 0.7%, 1.1% and 2.3% respectively;
and step four, controlling the fermentation temperature to be 35 ℃, controlling the pH to be 4.0, fermenting for 3 days, and drying at 40 ℃ to obtain the Chinese herbal medicine fermented feed additive.
The Chinese herbal medicine fermented feed additive is mixed with common feed for use; the mass ratio of the Chinese herbal medicine fermented feed additive to the common feed is 0.2 percent to 99.8 percent.
Example 4
(1) Weaned piglet feeding test
Selecting 120 piglets of 21-day-old ternary hybrid weaned piglets with similar gestation times and weight, wherein each half of the piglets is male and female, the test adopts single-factor complete test design, the piglets are randomly divided into 6 groups, each group is provided with 4 repetitions, and each repetition is provided with 5 piglets. The control group was fed with the basic daily ration, the group of example 1, the group of example 2, the group of example 3, the antibiotic group (10 mg/Kg of colistin sulfate +20mg/Kg of oxytetracycline) and the non-fermented compound Chinese herbal medicine group (0.4% Chinese herbal medicine). The test period was 30 days. The results are shown in Table 1.
TABLE 1 Effect of fermented Chinese herbal medicine on growth of weaned piglets
| Group of | Initial weight/Kg | Final weight/Kg | Average daily gain/g | Material to weight ratio | Rate of diarrhea/%) |
| Control group | 190.84±0.51 | 323.31±2.83 | 220.79±13.23 | 1.87±0.13 | 10.29±0.51 |
| Example 1 | 192.27±0.34 | 357.65±3.72 | 275.63±10.16 | 1.62±0.08 | 0.92±0.72 |
| Example 2 | 189.49±0.62 | 352.98±0.97 | 272.35±8.38 | 1.67±0.13 | 0.97±0.35 |
| Example 3 | 192.65±0.45 | 359.86±2.41 | 278.68±12.03 | 1.64±0.06 | 1.05±0.66 |
| Antibiotic group | 191.87±0.65 | 352.29±2.05 | 267.37±10.88 | 1.79±0.15 | 0.94±0.37 |
| Compound Chinese herbal medicine | 193.48±0.55 | 338.58±3.51 | 241.83±11.59 | 1.82±0.11 | 8.42±0.49 |
As can be seen from Table 1, the average daily gain of the weaned piglets is significantly higher than that of the control group and the unfermented compound Chinese herbal medicine, the material weight ratio and the diarrhea rate are both significantly lower than those of the control group and the unfermented compound Chinese herbal medicine, but the difference with the antibiotic group is not significant. The fermented Chinese herbal medicine can improve the digestibility of nutrient substances, improve the growth performance of weaned pigs and reduce the diarrhea rate, thereby achieving the aims of resisting bacteria and diminishing inflammation.
(2) Feeding effect of broiler chicken
Selecting healthy and active 300 feathers of 1-day-old AA broilers with similar body weight, randomly dividing the feathers into 5 groups, and repeating the feathers for 3 groups and 20 feathers for each group (half of male and half of female). The control group was the basal diet, example 1, example 2, and example 3 antibiotic groups (20 mg/Kg oxytetracycline base was added to the basal diet). The method adopts on-net flat culture, a material barrel and sufficient drinking water, and free feeding and drinking water are adopted to carry out vaccination according to conventional immunization. Test period 50 d. The results are shown in Table 2.
TABLE 2 influence of fermented Chinese herbal medicine on growth of broiler chickens
| Group of | End weight/g | Average daily gain/g | Survival rate/% | Meat ratio of materials | Incidence rate/%) |
| Control group | 1497.83±9.31 | 31.65±1.58 | 93.07±4.24 | 2.54±0.06 | 15.29±2.34 |
| Example 1 | 1708.52±11.63 | 37.55±0.73 | 99.41±5.63 | 2.17±0.08 | 1.09±0.11 |
| Example 2 | 1724.67±9.52 | 38.12±1.08 | 99.57±4.38 | 2.09±0.05 | 1.34±0.17 |
| Example 3 | 1713.26±10.55 | 36.83±0.94 | 99.26±5.08 | 2.14±0.03 | 1.21±0.02 |
| Antibiotic group | 1681.64±5.12 | 35.91±1.17 | 97.43±6.81 | 2.39±0.05 | 5.07±1.61 |
As can be seen from Table 2, compared with the control group and the antibiotic group, the fermented Chinese herbal medicine feed additive can significantly improve the daily gain and the survival rate of the broiler chickens and reduce the feed conversion ratio and the incidence of the broiler chickens. The fermented Chinese herbal medicine feed additive can promote the growth of the broiler chickens and reduce the morbidity of the broiler chickens.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (7)
1. An antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive is characterized by comprising Chinese herbal medicine fermented raw materials formed by fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea; the mass ratio of fructus forsythiae to radix isatidis to rhizoma atractylodis macrocephalae to fructus aurantii immaturus to fructus crataegi to echinacea purpurea is 2-3:6-10:4-7:4-9:20-26: 16-19; adding mixed fermentation strains into the Chinese herbal medicine fermentation raw materials; the mixed fermentation strain comprises bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus.
2. The Chinese herbal medicine fermented feed additive for resisting bacteria and diminishing inflammation as claimed in claim 1, wherein the mass ratio of the inoculation amount of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus in the mixed fermentation strain in the Chinese herbal medicine fermentation raw materials is 0.2-0.3%, 0.5-0.8%, 1-1.3% and 2-2.5% respectively.
3. The antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive as claimed in claim 2, wherein the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the rhamnose lactobacillus are all cultured in a liquid culture medium to a logarithmic phase, and then the bacterial liquid is inoculated into the Chinese herbal medicine fermented raw materials according to an inoculation amount of 0.1-3% and is stirred uniformly.
4. The antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive according to claim 1, wherein the culture medium of bacillus subtilis, bacillus amyloliquefaciens and bacillus belgii is: 6g/L of yeast powder, 9g/L of trypsin vein, 9g/L of sodium chloride, 10g/L of CMC-Na and 6 +/-0.6 of pH; the culture medium of the rhamnose lactobacillus is 10g/L beef extract, 10g/L peptone, 10g/L yeast extract, 20g/L tomato juice, 10/L glucose, 0.5ml/L Tween, 17g/L calcium carbonate and 0.1g/L bromocresol green.
5. The antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive according to claim 1, wherein the mass ratio of the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea is 2.5:7:5:6:22: 18.
6. A preparation method of an antibacterial and anti-inflammatory Chinese herbal medicine fermented feed additive is characterized by comprising the following steps:
weighing dried fructus forsythiae, radix isatidis, bighead atractylodes rhizome, immature bitter orange, hawthorn and echinacea purpurea, mixing and crushing the fructus forsythiae, the radix isatidis, the bighead atractylodes rhizome, the immature bitter orange, the hawthorn and the echinacea purpurea according to the mass ratio of 2-3:6-10:4-7:4-9:20-26:16-19, and then putting the mixture into a fermentation tank;
step two, adding a bacterial liquid of bacillus subtilis, bacillus amyloliquefaciens, bacillus belgii and lactobacillus rhamnosus cultured to a logarithmic phase into a fermentation tank, wherein the inoculation amounts of the bacillus subtilis, the bacillus amyloliquefaciens, the bacillus belgii and the lactobacillus rhamnosus are 0.2-0.3%, 0.5-0.8%, 1-1.3% and 2-2.5% respectively;
and step four, controlling the fermentation temperature to be 35 ℃, controlling the pH to be 4.0, fermenting for 2-3 days, and drying at 35-45 ℃ to obtain the Chinese herbal medicine fermented feed additive.
7. The Chinese herbal medicine fermented feed additive for resisting bacteria and diminishing inflammation as claimed in claim 1, wherein the Chinese herbal medicine fermented feed is mixed with a common feed for use; the mass ratio of the Chinese herbal medicine fermented feed additive to the common feed is 0.1-0.2% and 99.8-99.9%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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