CN106135712A - Mink specific complex probiotics fermention herbal health care oral liquid and preparation method - Google Patents
Mink specific complex probiotics fermention herbal health care oral liquid and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of mink specific complex probiotics fermention herbal health care oral liquid and preparation method, it is formulated according to volume ratio 1:1 2 with composite probiotics ferment liquid by Chinese medicine extraction liquid；With and preparation method thereof.Chinese herbal medicine is matched with compound probiotic, can play and control the purpose supporting synchronization, while treatment viral disease, improve animal mink immunity of organisms；The effect of preventing and treating diseases can not only be played, the symbiotic fermentation effect of the lactic acid bacteria in health promoting liquid, bacillus cereus can also be promoted, and produce substantial amounts of intermediate product, such as glucuronic acid, gluconic acid, acetic acid, lactic acid, aminoacid etc., antibiotic and chemistry disease-resistant drug can be replaced within the specific limits, reach real green ecological cultivation.
Description
Technical field
The present invention relates to a kind of mink specific complex probiotics fermention herbal health care oral liquid and preparation method.
Background technology
Plant (family) is long-term in breeding process adds antibiotic in feedstuff, adds the control not focusing on breeding environment
System, pollutes serious, only can't be so that cultivated animals resists pathogenic bacteria, viral infecting completely by probiotics preparation.In a large number
Experimentation shows, probiotic bacteria microbial inoculum has the effect dynamically regulating animal gastrointestinal tract flora ecosystem.There is promotion animal
Growth promoter, improve food conversion ratio, improve animal products quality, strengthen animal immunizing power, improve Anti-bacterium, the energy of antiviral
Power.But, many Chinese medicine research data show, Chinese medicine also has the effect such as prevention, treatment viral diarrhea, also has rush simultaneously
Enter animal growth, strengthen Immune Function In Animals.After the two is compound, directly utilizes beneficial flora fermentation of Chinese herbal medicine, can destroy
The cell wall of Chinese herbal medicine, is greatly improved the utilization rate of the active ingredient of Chinese herbal medicine, and produces multiple growth promotion during the fermentation
The factor and new material, it is possible to play the drug effect improving Chinese herbal medicine, will further improve the immunity of animal, improves product product
Matter, replaces antibiotic diseases prevention, cures the disease, reduce residue of veterinary drug, promote environmental benefit and ecological benefits, really realize ecological cultivation
It is China or even global common development trend.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of mink specific complex probiotics fermention
Herbal health care oral liquid and preparation method.
For solving above-mentioned technical problem, the present invention program includes:
A kind of mink specific complex probiotics fermention herbal health care oral liquid, wherein, by Chinese medicine extraction liquid and compound benefit
Raw fermented liquid is formulated according to volume ratio 1:1-2.
Described mink specific complex probiotics fermention herbal health care oral liquid, wherein, above-mentioned composite probiotics ferment
Liquid includes that the weight ratio of lactic acid bacteria and bacillus cereus, lactic acid bacteria and bacillus cereus is 1:1.
Described mink specific complex probiotics fermention herbal health care oral liquid, wherein, above-mentioned composite probiotics ferment
Liquid includes the lactic acid bacteria of 10 parts-20 parts and 10 parts of-20 parts of bacillus cereuss.
Described mink specific complex probiotics fermention herbal health care oral liquid, wherein, said herbal medicine extracting solution is
It is prepared from by the Radix Astragali, Fructus Forsythiae, Radix Isatidis, Flos Lonicerae, Flos Chrysanthemi；
As follows according to mass fraction: the Radix Astragali of 20 parts-30 parts, the Fructus Forsythiae of 20 parts-30 parts, the Radix Isatidis of 20-part 30 parts, 20
The Flos Lonicerae of part-30 parts and the Flos Chrysanthemi of 20 parts-30 parts.
A kind of preparing such as the method for described mink specific complex probiotics fermention herbal health care oral liquid, it includes preparation
The step of composite probiotics ferment liquid and the step preparing extracts of Chinese herbal medicine, then by the standby composite probiotics ferment liquid of preparation
Mix mutually with extracts of Chinese herbal medicine.
Described method, wherein, the above-mentioned step preparing composite probiotics ferment liquid includes:
A, activation preserve slant strains, cultivate lactic acid bacteria and bacillus cereus respectively by LB culture medium, pH be 7-7.5,
100-130 DEG C of autoclaving 20 minutes-40 minutes, cultivate 12 hours-20 hours under 30-38 DEG C of condition of culture；
B, the level liquid amplification culture that lactic acid bacteria, bacillus cereus are carried out respectively, inoculum concentration 10%-20%；
C, the lactic acid bacteria after level liquid amplification culture, bacillus cereus are carried out respectively secondary liquid amplification culture, inoculation
Amount 10%-20%；
D, by lactic acid bacteria, fermentation of bacillus liquid by weight for 1:1 ratio mix, obtain composite probiotics ferment
Liquid.
Described method, wherein, the above-mentioned step preparing extracts of Chinese herbal medicine includes:
E, corresponding Chinese herbal medicine powder is broken to 60 mesh-80 mesh, weighs corresponding Chinese herbal medicine powder according to weight component last fully
It is mixed to get Chinese herbal medicine powder；
F, the water of Chinese herbal medicine powder and 300 parts-600 parts is put in extraction pot, soak 5 hours-15 hours；Post-heating is extremely
40 DEG C-100 DEG C are extracted 10 hours-12 hours, are collected by filtration and obtain extracting solution；Then the medicinal residues in extraction pot add
300 parts of-600 parts of water, extract 10 hours-15 hours at 40 DEG C-100 DEG C；
J, merge twice extracting solution, under the conditions of 30 DEG C-70 DEG C, be evaporated to the 10%-20% of original volume, in obtaining
Medical herbs extracting solution.
Described method, wherein, concrete also the including of said method: add compound benefit in the extracts of Chinese herbal medicine obtained
Raw fermented liquid stroke mixed liquor, cultivated 12 hours-36 hours under the conditions of 25 DEG C-35 DEG C, opened stirring 5min every 12 hours,
Rotating speed is 120r/min, and liquid pH value to be mixed is down to 3.0-3.2, can stop fermentation, after stirring 30min, by four layer of 100 purpose
Filter-cloth filtering obtains described mink specific complex probiotics fermention herbal health care oral liquid.
The invention provides a kind of mink specific complex probiotics fermention herbal health care oral liquid and preparation method, in inciting somebody to action
Medical herbs matches with compound probiotic, can play and control the purpose supporting synchronization, improves animal while treatment viral disease
Mink immunity of organisms；Antibiotic and chemistry disease-resistant drug can be replaced within the specific limits, reach real green ecological cultivation.
Illustrate that below in conjunction with test the safety of composite probiotics ferment herbal health care liquid of the present invention is imitated with preventing and treating diseases
Really.
Compound probiotic herbal health care liquid of the present invention is in Qingdao university through rat acute toxicity test, and experimental group is 50
Rat, feeds 21 days by 0.6ml/10g body weight；Blank group is 50 rats, raises according to routine.
Conclusion The results shows that experimental group rat all survives, activity freely, hair smoothing, diet is normal, respiratory tract, eye and
Outside oral cavity etc. is without secretions, not finding any poisoning symptom, body weight all has increase.Compare with blank group, experimental group
Rat body weight substantially increases (P ＜ 0.001).21 day observation period terminated, by rat dislocation put to death dissect, the perusal heart, liver,
The internal organs such as spleen, lung, kidney, brain, intestinal, small intestinal are showed no obvious abnormalities performance.It addition, rat chest gland coefficient and Spleen coefficient result table
Bright, experimental group compares with blank group, and experimental group Rats Spleen coefficient substantially increases (P ＜ 0.01), and the prompting present invention answers
Closing probiotics fermention herbal health care liquid and have immunological enhancement, true border non-toxic type material, degree of safety is big.
The Chinese herbal medicine leachate of the present invention is after multiple probiotics fermention, and color all has clear improvement.Original hardship
Taste composition is decreased obviously, and has and significantly streaks sweet taste, flavour alcohol and tasty and refreshing, by the antioxidation of chemiluminescence determination health promoting liquid
Greatly improve.
The composite probiotics ferment herbal health care liquid of the present invention contains substantial amounts of Chinese herbal medicine material, as free amino acid,
Chlorophyll, carotene, enzyme, glycine betaine, vitamin A, vitamin B, vitamin C, vitamin E, Citrin and inorganic salt, micro-
The hundreds of kind of extractum such as secondary element；These Chinese herb medicines can not only play the effect of preventing and treating diseases, moreover it is possible to promotes in health promoting liquid
Lactic acid bacteria, the symbiotic fermentation effect of bacillus cereus, and produce substantial amounts of intermediate product, such as glucuronic acid, gluconic acid, vinegar
Acid, lactic acid, aminoacid etc..
Accompanying drawing explanation
Fig. 1 is that the composite probiotics ferment herbal health care liquid of variable concentrations affects schematic diagram to mink body weight；
Fig. 2 is the OD value schematic diagram of the little mink of different disposal group；
Detailed description of the invention
The invention provides a kind of mink specific complex probiotics fermention herbal health care oral liquid and preparation method, for making
The purpose of the present invention, technical scheme and effect are clearer, clear and definite, and the present invention is described in more detail below.Should be appreciated that
Specific embodiment described herein only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
The raw material preparing this composite probiotics ferment herbal health care liquid is by weight: the Radix Astragali 20 parts, Fructus Forsythiae 20 parts, plate
Blue root 20 parts, Flos Lonicerae 20 parts, Flos Chrysanthemi 20 parts, lactic acid bacteria 10 parts, bacillus cereus 10 parts.
The preparation process of composite probiotics ferment liquid is as follows:
(1) each slant strains that activation preserves, respectively shake-flask culture, lactic acid bacteria, bacillus cereus LB culture medium, pH7-
7.5,100-130 DEG C of autoclavings 20-40 minute, cultivate 12-15 hour at 30-38 DEG C；
(2) the level liquid amplification culture (50L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(3) the secondary liquid amplification culture (500L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(4) lactic acid bacteria, fermentation of bacillus liquid are mixed according to the following ratio: lactic acid bacteria 10 parts, bacillus cereus 10 parts.
Extracts of Chinese herbal medicine preparation process is as follows:
(1) Chinese herbal medicine powder being broken to 60 mesh, weigh by formula ratio, three-dimensional hybrid obtains Chinese herbal medicine powder；
(2) mixed Chinese herbal medicine powder, 300 parts of water are put in extraction pot respectively, soak 5 hours；
(3) it is heated to 40-100 DEG C to extract 10-12 hour；
(4) extracting solution is collected by filtration；
(5) secondary adds 300 parts of water in medicinal residues, and 40-100 DEG C is extracted 10 hours；
(6) merging twice extracting solution, 30-70 DEG C is evaporated to original volume 10%-20%, obtains coolant.
(7) in the coolant that step (6) is produced, lactic acid bacteria 10-20 part, bacillus cereus 10-20 part are added, at 25 DEG C-35
Under the conditions of DEG C, cultivate 12-36 hour, opened stirring 5min (rotating speed 120r/min) every 12 hours, treat that pH value is down to 3.0-3.2,
Fermentation can be stopped, after stirring 30min, put tank, release the bacterium solution filter-cloth filtering of four layer of 100 mesh and obtain fermentation filtrate, aseptic
Tinning, is packaged to be composite probiotics ferment herbal health care liquid.
Embodiment 2
The raw material preparing this composite probiotics ferment herbal health care liquid is by weight: the Radix Astragali 25 parts, Fructus Forsythiae 25 parts, plate
Blue root 25 parts, Flos Lonicerae 25 parts, Flos Chrysanthemi 25 parts, lactic acid bacteria 15 parts, bacillus cereus 15 parts.
The preparation process of composite probiotics ferment liquid is as follows:
(1) each slant strains that activation preserves, respectively shake-flask culture, lactic acid bacteria, bacillus cereus LB culture medium, pH7-
7.5,100-130 DEG C of autoclavings 20-40 minute, cultivate 15 hours at 30-38 DEG C；
(2) the level liquid amplification culture (50L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(3) the secondary liquid amplification culture (500L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(4) lactic acid bacteria, fermentation of bacillus liquid are mixed according to the following ratio: lactic acid bacteria 15 parts, bacillus cereus 15 parts.
Extracts of Chinese herbal medicine preparation process is as follows:
(1) Chinese herbal medicine powder being broken to 60 mesh, weigh by formula ratio, three-dimensional hybrid obtains Chinese herbal medicine powder；
(2) mixed Chinese herbal medicine powder, 300 parts of water are put in extraction pot respectively, soak 10 hours；
(3) it is heated to 40-100 DEG C to extract 10-12 hour；
(4) extracting solution is collected by filtration；
(5) secondary adds 300 parts of water in medicinal residues, and 40-100 DEG C is extracted 12 hours；
(6) merging twice extracting solution, 30-70 DEG C is evaporated to original volume 10%-20%, obtains coolant.
(7) in the coolant that step (6) is produced, lactic acid bacteria 15 parts, bacillus cereus 15 parts are added, 25 DEG C of-35 DEG C of conditions
Under, cultivate 12-36 hour, opened stirring 5min (rotating speed 120r/min) every 12 hours, treat that pH value is down to 3.0-3.2, Ji Keting
Only fermentation, puts tank after stirring 30min, releases the bacterium solution filter-cloth filtering of four layer of 100 mesh and obtains fermentation filtrate, aseptic canning,
It is packaged to be composite probiotics ferment herbal health care liquid.
Embodiment 3
The raw material preparing this composite probiotics ferment herbal health care liquid is by weight: the Radix Astragali 30 parts, Fructus Forsythiae 30 parts, plate
Blue root 30 parts, Flos Lonicerae 30 parts, Flos Chrysanthemi 30 parts, lactic acid bacteria 20 parts, bacillus cereus 20 parts.
The preparation process of composite probiotics ferment liquid is as follows:
(1) each slant strains that activation preserves, respectively shake-flask culture, lactic acid bacteria, bacillus cereus LB culture medium, pH7-
7.5,100-130 DEG C of autoclavings 20-40 minute, cultivate 20 hours at 30-38 DEG C；
(2) the level liquid amplification culture (50L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(3) the secondary liquid amplification culture (500L tank) of lactic acid bacteria, bacillus cereus, inoculum concentration 10%-20%；
(4) lactic acid bacteria, fermentation of bacillus liquid are mixed according to the following ratio: lactic acid bacteria 20 parts, bacillus cereus 20 parts.
Extracts of Chinese herbal medicine preparation process is as follows:
(1) Chinese herbal medicine powder being broken to 60 mesh, weigh by formula ratio, three-dimensional hybrid obtains Chinese herbal medicine powder；
(2) mixed Chinese herbal medicine powder, 300 parts of water are put in extraction pot respectively, soak 15 hours；
(3) it is heated to 40-100 DEG C to extract 10 hours；
(4) extracting solution is collected by filtration；
(5) secondary adds 300 parts of water in medicinal residues, and 40-100 DEG C is extracted 15 hours；
(6) merging twice extracting solution, 30-70 DEG C is evaporated to original volume 10%-20%, obtains coolant.
(7) in the coolant that step (6) is produced, lactic acid bacteria 10-20 part, bacillus cereus 10-20 part are added, at 25 DEG C-35
Under the conditions of DEG C, cultivate 12-36 hour, opened stirring 5min (rotating speed 120r/min) every 12 hours, treat that pH value is down to 3.0-3.2,
Fermentation can be stopped, after stirring 30min, put tank, release the bacterium solution filter-cloth filtering of four layer of 100 mesh and obtain fermentation filtrate, aseptic
Tinning, is packaged to be composite probiotics ferment herbal health care liquid.
In order to be further described mink composite probiotics ferment herbal health care oral liquid to mink body weight, immunity
With the impact of intestinal health, below mink is carried out packet comparison.
Test mink, black for Dalian, agricultural science and technology company limited Fur Animal Feeding base, Qingdao provide.Choose
150 age in days son ermines 60, are randomly divided into 6 groups by sex and body weight, often group 5 repetition, often repeat 2, male and female half and half, test from
On April 20th, 2016 rises, May 24 end of day, 35 days altogether.Often organizing little mink divides cage to feed, free choice feeding and drinking-water.
Separate lactic acid bacteria and the live strain of bacillus cereus of preservation with immunization experiment room by Qingdao university animal microorganism.
Colony counting (colony counting method)
It is placed in the wide mouthed bottle filling 250mL normal saline with taking 25mL bacterium solution through the graduated cylinder of high pressure in super-clean bench,
Fully shake up the proportional diluent for 1:10.It is placed in the suction pipe absorption 1.0mL through high pressure that volume is 1.0mL and fills
In the normal saline of 9mL, carry out ten times of dilutions, repeat aforesaid operations until bacterium solution extension rate is 109.Draw with suction pipe
The bacterium solution of 1.0mL is placed in the plate after high pressure, baking, and the Nutrient agar that simultaneously will be cooled to about 46 DEG C pours plate into
In, move plate simultaneously and make solution be sufficiently mixed.Cultivate 24h counting in flat board is placed in calorstat, repeat to do three times.Counting public affairs
Formula: the average colony number * dilution number of degrees that every milliliter of colony-forming units (cfu)=same dilution factor three times repeats.
Inoculation
Experimental animal is randomly divided into A, B, C, D, E, F six groups, often 5 repetitions of group.Mink is carried out immunity, the most every
The antigen of mink injection equal-volume mixing and Freund's complete adjuvant 0.1mL.After one week, every mink injection equal-volume mixes
The antigen closed and incomplete Freund's adjuvant adjuvant 0.1mL, the antigen of every mink duplicate injection equal-volume mixing after being spaced one week
With incomplete Freund's adjuvant adjuvant 0.1mL.
Processing mink gavage, table 1 is the Different treatments to test mink
The impact on mink body weight of the different tests group
Interpretation of result: as shown in Figure 1, be can be seen that by broken line graph the original body mass of mink is close, and gavage processes one week
The body weight gap of rear different tests group mink is still little, starts obvious from two Zhou Yihou gaps, feed lactic acid bacteria group,
Bacillus cereus group, Chinese herbal medicine group, the body weight of composite probiotics ferment herbal health care liquid group mink is all higher than gavage normal saline
Matched group, and the little mink body weight of gavage composite probiotics ferment herbal health care liquid group is higher.
The impact on mink internal organs index of the different tests group, table 2 be the little mink of different disposal group internal organs index (
N=5)
Table 2
Data analysis: the internal organs of mink are carried out statistical analysis with spss software, the method using multiple comparisons.By
Table 2 draws, mink each internal organs index of composite probiotics ferment herbal health care liquid group is extremely notable (P ＜ compared with matched group
0.01) and be significantly higher than other experimental grouies (P ＜ 0.05), bacillus cereus, lactic acid bacteria, each internal organs of Chinese drug-treated group independent role group
Weight be also significantly greater than matched group (P ＜ 0.05) composite probiotics ferment herbal health care liquid liquid group be significantly higher than matched group and
Probiotic bacteria and Chinese drug-treated group (P ＜ 0.05).
Seroimmunity fine jade expands result, and table 3 is serum bursal disease virus antibody titer.
Table 3
Calculated by table 3: each test group serum titer relatively matched group increases 29%, 24%, 11% respectively,
17%, 14%；30%, 26%, 15%, 8%, 12%.Composite probiotics ferment herbal health care liquid liquid group mink can be drawn
Serum bursal antibody content has obvious facilitation compared with other test group.
ELISA test result analysis, table 4 is the OD value of the little mink of different disposal group
Shown in table 4 and Fig. 2, it can be deduced that OD value other test group in addition to E group recorded for the first time all compares
Close, i.e. after first time immunity, the antibody content difference in mink body is inconspicuous；The OD value that second time is recorded by test is still
Relatively, i.e. the antibody content in mink body is more or less the same；Composite probiotics ferment herbal health care liquid after immunity for the third time
Group OD numerical value, apparently higher than other test group, illustrates that its internal antibody content numerical value compared with other test group raises bigger.
The different tests group mucosal thickness on mink jejunum, the impact of villus length
Draw at light Microscopic observation, bacillus cereus group, Chinese drug-treated group, composite probiotics ferment herbal health care liquid liquid group and right
More complete and well arranged according to group mink jejunal mucous membrane structure all ratios, intestinal villus is the most neat.Visible have obvious branch,
The striated border structure straight uniform of mucous membrane of small intestine epithelial surface.Compared with matched group, composite probiotics ferment herbal health care liquid
Organizing the arrangement of little mink jejunal mucous membrane fine hair more tight, fine hair is long.
This result of the test shows, no matter gives mink gavage bacillus cereus group, lactic acid bacteria group, Chinese drug-treated group, or gavage is multiple
Close probiotics fermention herbal health care liquid group and all can promote the growth of mink, but gavage composite probiotics ferment herbal health care
The mink body weight of liquid group after two weeks compared with other test group much larger than other test group.
The impact on mink internal organs index of the different tests group
Can be shown that composite probiotics ferment herbal health care liquid group is in the growth promoting little mink internal organs by test
Aspect difference compared with matched group is extremely notable (p < 0.01).Gavage lactic acid bacteria group, bacillus cereus group and Chinese drug-treated group are to organ index
Impact significant difference (p < 0.05) compared with matched group.Show that composite probiotics ferment herbal health care liquid group is in mink
Dirty index aspect effect is more notable.
The impact on bursal antibody titer of the different tests group
Mink antibody titer significant difference compared with matched group of this result of the test display different tests group, but compound benefit
The serum titer relatively matched group of raw bacterium fermentation of Chinese herbal medicine health promoting liquid group mink increases 29% He respectively for the first time and for the second time
30%, change in value is bigger compared with other test group.Show that compound probiotic herbal health care liquid is in the titer strengthening antibody
Aspect effect is more notable.
So, the patent of the present invention growth-weight gain to mink, the growth of internal organs, the rising of chicken bursal antibody titer
And the Microvillares length of intestinal and intestinal wall thickness all have obvious facilitation, the performance of mink is greatly improved.
Certainly, only presently preferred embodiments of the present invention described above, the present invention is not limited to enumerate above-described embodiment, should
When explanation, any those of ordinary skill in the art under the teaching of this specification, made all equivalent substitute, bright
Aobvious variant, within all falling within the essential scope of this specification, ought to be protected by the present invention.
Claims (8)
1. a mink specific complex probiotics fermention herbal health care oral liquid, is characterised by, it is by Chinese medicine extraction liquid and answers
Close probiotics fermention liquid formulated according to volume ratio 1:1-2.
Mink specific complex probiotics fermention herbal health care oral liquid the most according to claim 1, it is characterised in that on
State composite probiotics ferment liquid and include that the weight ratio of lactic acid bacteria and bacillus cereus, lactic acid bacteria and bacillus cereus is 1:1.
Mink specific complex probiotics fermention herbal health care oral liquid the most according to claim 2, it is characterised in that on
State composite probiotics ferment liquid and include the lactic acid bacteria of 10 parts-20 parts and 10 parts of-20 parts of bacillus cereuss.
Mink specific complex probiotics fermention herbal health care oral liquid the most according to claim 1, it is characterised in that on
Stating extracts of Chinese herbal medicine is to be prepared from by the Radix Astragali, Fructus Forsythiae, Radix Isatidis, Flos Lonicerae, Flos Chrysanthemi；
As follows according to mass fraction: the Radix Astragali of 20 parts-30 parts, the Fructus Forsythiae of 20 parts-30 parts, the Radix Isatidis of 20-part 30 parts, 20 parts-
The Flos Lonicerae of 30 parts and the Flos Chrysanthemi of 20 parts-30 parts.
5. the method preparing mink specific complex probiotics fermention herbal health care oral liquid as claimed in claim 1, its
Including preparing the step of composite probiotics ferment liquid and preparing the step of extracts of Chinese herbal medicine, then standby compound prebiotic by prepare
Fermented liquid mixes mutually with extracts of Chinese herbal medicine.
Method the most according to claim 5, it is characterised in that the above-mentioned step preparing composite probiotics ferment liquid includes:
A, activation preserve slant strains, cultivate lactic acid bacteria and bacillus cereus respectively by LB culture medium, are 7-7.5,100-130 at pH
DEG C autoclaving 20 minutes-40 minutes, cultivate 12 hours-20 hours under 30-38 DEG C of condition of culture；
B, the level liquid amplification culture that lactic acid bacteria, bacillus cereus are carried out respectively, inoculum concentration 10%-20%；
C, the lactic acid bacteria after level liquid amplification culture, bacillus cereus are carried out respectively secondary liquid amplification culture, inoculum concentration
10%-20%；
D, by lactic acid bacteria, fermentation of bacillus liquid by weight for 1:1 ratio mix, obtain composite probiotics ferment liquid.
Method the most according to claim 5, it is characterised in that the above-mentioned step preparing extracts of Chinese herbal medicine includes:
E, corresponding Chinese herbal medicine powder is broken to 60 mesh-80 mesh, weighs according to weight component that corresponding Chinese herbal medicine powder is last is sufficiently mixed
Obtain Chinese herbal medicine powder；
F, the water of Chinese herbal medicine powder and 300 parts-600 parts is put in extraction pot, soak 5 hours-15 hours；Post-heating is to 40
DEG C-100 DEG C extract 10 hours-12 hours, are collected by filtration and obtain extracting solution；Then the medicinal residues in extraction pot add 300
-600 parts of water of part, extract 10 hours-15 hours at 40 DEG C-100 DEG C；
J, merge twice extracting solution, under the conditions of 30 DEG C-70 DEG C, be evaporated to the 10%-20% of original volume, obtain Chinese herbal medicine
Extracting solution.
Method the most according to claim 7, it is characterised in that concrete also the including of said method: to the Chinese herbal medicine obtained
Extracting solution adds composite probiotics ferment liquid stroke mixed liquor, cultivation 12 hours-36 hours under the conditions of 25 DEG C-35 DEG C, often
Opened stirring 5min every 12 hours, rotating speed is 120r/min, and liquid pH value to be mixed is down to 3.0-3.2, can stop fermentation, stirring
After 30min, obtain described mink specific complex probiotics fermention herbal health care oral liquid with the filter-cloth filtering of four layer of 100 mesh.
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| CN106993580A (en) * | 2017-04-28 | 2017-08-01 | 广西乐业康辉生态养殖专业合作社 | It is a kind of to improve the chicken-raising method of chicken meat |
| CN107156027A (en) * | 2017-04-28 | 2017-09-15 | 广西乐业康辉生态养殖专业合作社 | A kind of chicken-raising method |
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| CN106490306A (en) * | 2016-12-08 | 2017-03-15 | 青岛农业大学 | A kind of preparation method of fur-bearing animal compound micro-ecological preparation |
| CN106993580A (en) * | 2017-04-28 | 2017-08-01 | 广西乐业康辉生态养殖专业合作社 | It is a kind of to improve the chicken-raising method of chicken meat |
| CN107156027A (en) * | 2017-04-28 | 2017-09-15 | 广西乐业康辉生态养殖专业合作社 | A kind of chicken-raising method |
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