CN106581310A - Traditional Chinese medicine micro-ecological preparation for feed, and preparation method and application thereof - Google Patents
Traditional Chinese medicine micro-ecological preparation for feed, and preparation method and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/233—Bupleurum
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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Abstract
The invention discloses a traditional Chinese medicine micro-ecological preparation for a feed, and a preparation method and application thereof. The traditional Chinese medicine micro-ecological preparation for the feed comprises a traditional Chinese medicine fermentation extract, saccharomyces boulardii, bacillus licheniformis and enterococcus faecalis in a ratio of (1.5-2.5)g:(5*10<9>-15*10<9>)CFU:(10*10<9>-30*10<9>)CFU:(0.5*10<9>-2.5*10<9>)CFU, wherein the traditional Chinese medicine fermentation extract is fermented by inoculating a traditional Chinese medicinal material with aspergillus niger and trichoderma koningii; the traditional Chinese medicinal material consists of radix astragali, cortex eucommiae, liquoric root, Chinese angelica, Chinese thorowax root, dahurian patrinia herb, purslane herb, figwortflower picrorhiza rhizome and halloysit, wherein the mass ratio is (1-10):(1-10):(1-10):(1-10):(1-10):(1-10):(1-5):(1-5). According to the invention, the traditional Chinese medicine is fermented by using the aspergillus niger and the trichoderma koningii, effective components in the traditional Chinese medicine are released in a relatively mild manner, active components of the traditional Chinese medicine are protected from being destroyed, and a new medicine effect and new nutrient substances are generated, thus facilitatingdigestion, absorption and utilization of an animal.
Description
Technical field
The invention belongs to technical field of microbial fermentation, more particularly to a kind of feedstuff Chinese medicine microecological preparation and its preparation
Method and application.
Background technology
In the breeding process of poultry, often there are the diseases such as digestive tract, respiratory tract, this has severely impacted the life of poultry
It is long, after animal infection disease, particularly infect after intestinal tract disease, body hydraulic performance decline, benefit is reduced, and becomes other health
The source of infection of domestic animal, and the remedying problems for being directed to livestock intestinal tract disease always perplex the difficult problem of numerous raisers, at present
Antibiotic therapy is mainly applied, but life-time service antibiotic or the drug resistance bacteria growing and drug resistant gene of abuse of antibiotics initiation turn
Move, the decline of humans and animals immunity of organisms, the increase of secondary dosage, drug residue, dual and autogenous infection, cause may be caused
A series of problems, such as quick effect, health is constituted a threat to.The animal food safety hidden danger that these factors cause enjoys consumption
Person pays close attention to.
At the same time, as the development and the progress of society of national economy, the living standard of the people are improved constantly, self is protected
The progressively enhancing of strong consciousness, proposes development green health cultivation, it is ensured that the safety of animal foodstuff, to meet domestic and international market pair
The quality standard of animal products, realizes the sustainable development of aquaculture.That pursuit quantity in the past must be changed, matter is careless about
The outdated ideas of amount, walk the road of science green cultivation.Guarantee the animal derived foods such as meat, egg, milk to high-quality, nutrition, safe direction
Development.
Chinese herbal medicine is the product of the distinctive Chinese medical theory of China and practice, and aboundresources is with a long history, is that the mankind are earliest
Using natural organic substance, according to incompletely statistics, have two in more than the 1000 kinds of Chinese herbal medicine used on current veterinary clinic
More than hundred kinds is utilized as raw materials of feed additives.Chinese herbal medicine has that feature is strong, toxic and side effects are little, drug residue is low, no
The features such as being also easy to produce Drug resistance.
Microorganism formulation is also called microbial modulator, is the preparation prepared according to microorganism principle.Its objective is adjustment
Microbial state treatment, keeps ecological balance, and the health level of poultry is improved, with the effect for reaching diseases prevention, cure the disease.
But, general Chinese herbal medicine often makes domestic animal feel that palatability is poor because the characteristic of itself, is unwilling or keeps away
Open edible, microbial ecological agent is often easy in its natural state inactivation, so, if preferably to utilize them, just need
Certain remanufacturing is carried out to them.
The content of the invention
It is an object of the invention to provide a kind of feedstuff Chinese medicine microecological preparation and preparation method and application, contains inside it
There are with enhance immunity, prevent and treat Chinese medicine ingredients and the multiple beneficial bacterium of dysentery.The Chinese medicine microecological preparation has significant
Immunoregulation effect, and have no toxic side effect, pollution-free, noresidue, do not produce drug resistance.
A kind of feedstuff Chinese medicine microecological preparation that the present invention is provided, it includes (1.5～2.5) g：(5×109～15 ×
109)CFU：(10×109～30 × 109)CFU：(0.5×109～2.5 × 109) CFU herb fermenting extract, Bradley yeast
Bacterium (Saccharomyces boulardii), Bacillus licheniformis (Bacillus licheniformis) and enterococcus faecalis
(Enterococcus faecalis)；The herb fermenting extract is by Chinese crude drug inoculated aspergillus niger (Aspergillus
Niger) form with trichodermaharzianum (Trichoderma koningii) fermentation, preparation process is as follows：The Chinese crude drug will be constituted
Each component drying, crush after mix, sterilize after soaking, access the mixed bacteria liquid of aspergillus niger and trichodermaharzianum, it is fermented,
It is obtained after drying and inactivation；The Chinese crude drug is (1～10) by mass ratio：(1～10)：(1～10)：(1～10)：(1～10)：
(1～10)：(1～5)：The Radix Astragali of (1～5), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum
Composition.
In above-mentioned feedstuff Chinese medicine microecological preparation, the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis
With the ratio of enterococcus faecalis concretely 1) -8) in any one：
1) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 2g：(5×
109～10 × 109)CFU：(10×109～20 × 109)CFU：(0.5×109～2.5 × 109)CFU；
2) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 2.5g：(5
×109～10 × 109)CFU：(15×109～30 × 109)CFU：(0.5×109～2.5 × 109)CFU；
3) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 1.5g：(5
×109～10 × 109)CFU：(10×109～20 × 109)CFU：(1.0×109～2.5 × 109)CFU；
4) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 1.5g：(5
×109～15 × 109)CFU：(15×109～30 × 109)CFU：(0.5×109～2.5 × 109)CFU；
5) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis for (1.5～
2.5)g：10×109CFU：(10×109～20 × 109)CFU：2.5×109CFU；
6) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 2g：10×
109CFU：20×109CFU：2.5×109CFU；
7) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 2.5g：10
×109CFU：20×109CFU：2.5×109CFU；
8) ratio of the herb fermenting extract, saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis is 1.5g：10
×109CFU：10×109CFU：2.5×109CFU。
The bacterial content of the saccharomyces boulardii concretely 10 × 109CFU/g；The bacterial content tool of the Bacillus licheniformis
Body can be 20 × 109CFU/g；The bacterial content of the enterococcus faecalis concretely 5 × 109CFU/g。
In above-mentioned feedstuff Chinese medicine microecological preparation, the Chinese crude drug concretely following 1) -7) in any one：
1) Chinese crude drug is 10 by mass ratio：(8～10)：(3～6)：(4～7)：(7～9)：(1～3)：(1～3)：
The Radix Astragali of (3～5), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
2) Chinese crude drug is 10 by mass ratio：(5～10)：(1～4)：(4～7)：(8～10)：(4～6)：(1～3)：
The Radix Astragali of (3～5), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
3) Chinese crude drug is 10 by mass ratio：10：3：6：9：5：2：4 Radix Astragali, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, lose
Beans grass, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
4) Chinese crude drug is 5 by mass ratio：(4～6)：(4～7)：(1～4)：(6～8)：(1～3)：(3～5)：(2
～the Radix Astragali 4), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
5) Chinese crude drug is 5 by mass ratio：5：5：3：6：2：3：3 Radix Astragali, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae
Grass, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
6) Chinese crude drug is 3 by mass ratio：(2～4)：(6～9)：(8～10)：(2～4)：(8～10)：(2～5)：
The Radix Astragali of (1～3), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition；
7) Chinese crude drug is 3 by mass ratio：3：8：9：3：10：4：2 Radix Astragali, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae
Grass, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition.
In above-mentioned feedstuff Chinese medicine microecological preparation, in the soaking step, the mass ratio of the Chinese crude drug and the water
Can be 1：(1～1.5), concretely 1：1.
In the mixed bacteria liquid of the aspergillus niger and trichodermaharzianum, the concentration of aspergillus niger is (2.5 × 109～15 × 109)CFU/
ML, concretely 2.5 × 109CFU/mL；The concentration of trichodermaharzianum is (5 × 109～20 × 109) CFU/mL, concretely 5 ×
109CFU/mL.Specifically, the mixed bacteria liquid of the aspergillus niger and trichodermaharzianum by aspergillus niger bacterium solution and trichodermaharzianum bacterium solution according to
Volume ratio is 1：1 ratio is mixed to get；The concentration of the aspergillus niger bacterium solution concretely 10 × 109CFU/mL is described healthy and free from worry
The concentration of Trichoderma spp. bacterium solution concretely 10 × 109CFU/mL。
The aspergillus niger bacterium solution and the trichodermaharzianum bacterium solution are commercially available, conventional preparation method may also be employed and prepares
(with reference to aspergillus niger, the conventional manufacture method of trichodermaharzianum) is obtained, such as aspergillus niger, trichodermaharzianum respective training is inoculated in into respectively
In foster base, primary seed solution is cultivated, be transferred to secondary seed respectively when aspergillus niger, trichodermaharzianum grow into exponential phase and send out
Culture is enlarged in fermentation tank, stops culture when reaching exponential phase, into bacterium solution.
In the fermentation step, the ratio of the Chinese crude drug and the mixed bacteria liquid can be Chinese crude drug 100g：Mixed bacteria liquid
(10×109～35 × 109) CFU, concretely Chinese crude drug 100g：Mixed bacteria liquid 15 × 109CFU.The temperature of the fermentation can be
28～37 DEG C, concretely 28 DEG C；Fermentation time can be 24～72 hours, concretely 60 hours.
In the baking step, drying temperature can be 35～50 DEG C, concretely 40～50 DEG C, 40 DEG C or 50 DEG C；Time
It can be 24～48 hours, concretely 48 hours.
In the inactivation step, inactivation temperature can be 80 DEG C, and the time is 2 hours.
In above-mentioned feedstuff Chinese medicine microecological preparation, feedstuff Chinese medicine microecological preparation also includes pharmaceutic adjuvant；Institute
In stating Chinese medicine microecological preparation, the weight/mass percentage composition of the pharmaceutic adjuvant is 50%～98%, concretely 80%.The medicine
Selected according to the dosage form of the Chinese medicine microecological preparation with adjuvant, including but not limited to antioxidant, binding agent, correctivess
And carrier.Described binding agent includes but is not limited to syrup, refined honey, maltose, alcohol and starch slurry, and carrier can be cyclodextrin.
Invention further provides a kind of preparation method of above-mentioned feedstuff Chinese medicine microecological preparation, including following step
Suddenly：
(1) each component of the Chinese crude drug will be constituted successively after dried, crushed according to mass ratio mixing, Chinese medicine is obtained
Material；
(2) will be soaked in water in the Chinese crude drug, obtain immersion system；By the immersion system sterilize after inoculated aspergillus niger and
The mixed bacteria liquid of trichodermaharzianum bacterium, after fermented, drying and inactivation herb fermenting extract is obtained；
(3) by the fermented product extract, the saccharomyces boulardii, the Bacillus licheniformis and the enterococcus faecalis according to
The ratio mixing, you can obtain the feedstuff Chinese medicine microecological preparation.
Above-mentioned preparation method, in step (1), the temperature of the drying is 50～60 DEG C (such as 60 DEG C), and the time is 2～3h
(such as 2h).The mesh number of the crushing can be 80～120 mesh, such as 80 mesh.
In step (2), in the soaking step, the Chinese crude drug can be 1 with the mass ratio of the water：(1～1.5), tool
Body can be 1：1；The temperature of immersion can be 15～25 DEG C, concretely 25 DEG C；Time can be 12～24 hours, and concretely 24 is little
When.Also include the step of pH value of soak being adjusted between 6.5～7.5 after the immersion.
The immersion system also included the step of sterilizing to the immersion system before the inoculation.The sterilizing can be
Steam sterilization, sterilize 15～20min such as at 121 DEG C (such as 20min).
In the mixed bacteria liquid of the aspergillus niger and trichodermaharzianum, the concentration of aspergillus niger is (2.5 × 109～15 × 109)CFU/
ML, concretely 2.5 × 109CFU/mL；The concentration of trichodermaharzianum can be (5 × 109～20 × 109) CFU/mL, concretely 5
×109CFU/mL.Specifically, the mixed bacteria liquid of the aspergillus niger and trichodermaharzianum is pressed by aspergillus niger bacterium solution and trichodermaharzianum bacterium solution
It is 1 according to volume ratio：1 ratio is mixed to get；The concentration of the aspergillus niger bacterium solution is 10 × 109CFU/mL, the trichodermaharzianum
The concentration of bacterium solution is 10 × 109CFU/mL。
The aspergillus niger bacterium solution and the trichodermaharzianum bacterium solution are commercially available, conventional preparation method may also be employed and prepares
(with reference to aspergillus niger, the conventional manufacture method of trichodermaharzianum) is obtained, such as aspergillus niger, trichodermaharzianum respective training is inoculated in into respectively
In foster base, primary seed solution is cultivated, be transferred to secondary seed respectively when aspergillus niger, trichodermaharzianum grow into exponential phase and send out
Culture is enlarged in fermentation tank, stops culture when reaching exponential phase, into bacterium solution.
In the fermentation step, the ratio of the Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid (10
×109～35 × 109) CFU, concretely Chinese crude drug 100g：Mixed bacteria liquid 15 × 109CFU.The temperature of the fermentation can be 28
～37 DEG C, concretely 28 DEG C；Fermentation time can be 24～72 hours, concretely 60 hours.
In the baking step, drying temperature can be 35～50 DEG C, concretely 50 DEG C；Time can be 24～48 hours,
Concretely 48 hours.
In the inactivation step, inactivation temperature can be 80 DEG C, and the time can be 2 hours.
In step (3), the fermented extracted also included being crushed to 80～120 mesh before the mixing (such as 80 mesh)
The step of sieve.
Present invention also offers above-mentioned feedstuff Chinese medicine microecological preparation is following 1) -8) at least one of in application：
1) poultry feed intake is improved；
2) poultry daily gain is improved；
3) poultry body constitution is strengthened；
4) growth of animals or poultry is promoted；
5) livestock and poultry production performance is improved；
6) fowl poultry immune performance is improved；
7) poultry death rate is reduced；
8) poultry feedstuff-meat ratio is reduced.
Crude drug of the present invention：
The Radix Astragali：Sweet in the mouth slightly warm in nature；Return lung, spleen, liver and kidney channel；Polishing consolidating superficial resistance, torr sore myogenic；Cure mainly body void spontaneous perspiration, chronic diarrhea,
Proctoptosis.
The Cortex Eucommiae：It is sweet in the mouth, warm in nature；Can invigorating the liver and kidney, bone and muscle strengthening, antiabortive；For deficiency of the liver and kindey, soreness of waist and knee joint, flaccidity and weakness of the lower limbs, sun
Flaccidity withers frequent micturition；Liver and kidney is weak, gestation hemorrhage, frequent fetal movement, or habitual abortion；Hypertension.
Radix Glycyrrhizae：It is sweet in the mouth, mild-natured；Return 12 warps；With removing toxic substances, eliminating the phlegm, pain relieving, spasmolytic effect；It is raw with curing mainly laryngopharynx swelling and pain,
Cellulitis skin infection.
Radix Angelicae Sinensis：Warm in nature, sweet in the mouth is pungent；GUIXIN, liver, spleen channel；With benefiting blood and regulating blood circulation, menstruction regulating and pain relieving, moisturize laxation effect；Cure mainly
Menoxenia, amenorrhea stomachache, lump in the abdomen knot is poly-, metrorrhagia.
Radix Bupleuri：Abnormal smells from the patient is bitter, flat, nontoxic；Stagnation of QI in main trusted subordinate's the intestines and stomach, diet accumulation, cold and heat pathogen, push away Chen Zhixin；Long term usage
Make light of one's life by commiting suicide, improving eyesight, benefit essence；Cold, fever is cured mainly, alternate attack of chill and fever, malaria, stagnation of QI due to depression of the liver, sternal rib pain, proctoptosis, uterus comes off.
Herba Patriniae：Be slightly cold, pungent, hardship；Return stomach, large intestine, Liver Channel；Cure mainly：Heat-clearing and toxic substances removing, removing blood stasis and expelling pus.
Herba portulacae：It is sour in the mouth, cold in nature；There are heat-clearing and toxic substances removing, parasite killing and dissipate blood, cure mainly blurring of vision or mesh that pyretic toxicity causes
Raw nebula film swells and ache, and toxic-heat and blood stasis.
Rhizoma Picrorhizae：Cold in nature, bitter in the mouth；Return liver warp, stomach, large intestine channel；For reducing the asthenic fever, dispelling fever with infantile malnutrition, clearing away damp-heat.
Halloysitum Rubrum：It is sweet, puckery, sour, warm, nontoxic；Returns spleen Jing, stomach, heart channel, large intestine channel；Effect：Astringing intestine to stop diarrhea, astringing to arrest bleeding, receipts
Wet sore, granulation promoting；Cure mainly：Chronic diarrhea, chronic dysentery.
The present invention has the advantages that：
1st, the present invention adopts aspergillus niger and trichodermaharzianum fermented tcm, by effective ingredient in Chinese medicine in comparatively gentle mode
Discharge, protect active ingredient of Chinese herbs to exempt from destruction, and produce new drug effect and new nutrient substance so that animal is more easy to disappear
Change, absorb, utilize；
2nd, feedstuff Chinese medicine microecological preparation of the present invention can strengthen the immunocompetence of animal body, maintain animal intestines and stomach micro-
Biological flora is balanced, effectively preventing and treating livestock and poultry alimentary tract and Other diseases, especially the dysentery of preventing and treating domestic animal, promotes livestock birds health, fast
Grow fastly, meat, eggs and milk quality better, and with having no toxic side effect, pollution-free, noresidue, not produce the safeties such as drug resistance special
Point；The requirement that people keep the safety in production to meat, egg, milk is disclosure satisfy that, while the effects such as also reducing cultivation input；
3rd, feedstuff Chinese medicine microecological preparation of the invention is adapted to be used in each growth stage of livestock and poultry animal；And can
By improving using dosage, apply as veterinary drug.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, commercially obtain.
The CICC numberings of the saccharomyces boulardii Saccharomyces boulardii in following embodiments are 1850, the public
Can be commercially available from CICC.
The CICC numberings of Bacillus licheniformis Bacillus licheniformis are 10085, and the public can purchase from CICC
Can buy.
The ATCC numberings of enterococcus faecalis Enterococcus faecalis are 19433, and the public can buy from ATCC
Arrive.
The CICC numberings of aspergillus niger Aspergillus niger are 2103, and the public can be commercially available from CICC.
The CICC numberings of trichodermaharzianum Trichoderma koningii are 13009, and the public can be commercially available from CICC.
Embodiment 1, prepare feedstuff Chinese medicine microecological preparation
First, the preparation of Bradley yeast bacteria agent
Bradley yeast bacteria agent is prepared in accordance with the following steps：
(1) activation culture：Saccharomyces boulardii species are inoculated with activation medium, the activation culture in 31 ± 1 DEG C of environment
Obtain activated seed liquid within 42～54 hours；
(2) one-level spreads cultivation：The activated seed liquid is inoculated with first cell culture medium, the pH value of the first cell culture medium is
5.0, glucose content is 10%, and ventilating ratio is 0.6 during one-level spreads cultivation：1, under stirring, dissolved oxygen is 25%, temperature
Cultivate 15～20 hours for 31 ± 1 DEG C, obtain the one-level culture fluid that Bradley yeast wet thallus content is 4～5wt%；
(3) two grades spread cultivation：The one-level culture fluid is inoculated with secondary medium by 10～20% inoculum concentration, two grades
The pH value of culture medium is 5.0, and glucose content is 9%, two grades spread cultivation during ventilating ratio be 0.6：1, it is 31 ± 1 DEG C in temperature
Culture 6～10 hours, obtains two grades of culture fluid that Bradley yeast wet thallus content is 4～5wt%；
(4) fermentation culture：Two grades of culture fluid are inoculated with fermentation medium by 20% inoculum concentration, fermentation culture
The pH value of base is 5.0, and Starting glucose content is 0.5%, and ventilating ratio is 0.6 in sweat：1, residual sugar in fermentation medium
Content maintains 0.015～0.02%, temperature be 31 ± 1 DEG C, dissolved oxygen obtain Bradley yeast wet thallus for 20% fermentation culture
Content is the fermentation culture of 16～22g/100mL；
(5) fermentation culture is obtained into fresh yeast after separation, brine wash and dehydration, 1 is added in fresh yeast
The activity protecting agent (dextrin etc.) of～3wt%, post-drying smashes into powder；Or after blended pelletize, Jing two-step fluidized beds dry
It is dry, obtain moisture≤6wt%, viable count 10 × 109The dry Bradley yeast powder of the activity of CFU/g or granular preparation.
2nd, bacillus licheniformis agent is prepared
Bacillus licheniformis agent is prepared in accordance with the following steps：
(1) activation culture：Bacillus licheniformis strain is inoculated with activation medium, is activated in 31 ± 1 DEG C of environment
Culture obtains activated seed liquid in 48 hours；
(2) one-level spreads cultivation：The activated seed liquid is inoculated with first cell culture medium, the pH value of the first cell culture medium is
6.5, wherein sucrose, bean cake addition is every liter of 40 grams of consumption, and other are inorganic salt, and ventilating ratio is 0.9 during one-level spreads cultivation：
1, under stirring, dissolved oxygen is that 25%, temperature is cultivated 24 hours for 30 DEG C, obtains Bacillus licheniformis wet thallus content for 4
The one-level culture fluid of～5wt%；
(3) fermentation culture：The one-level culture fluid is inoculated with fermentation medium by 10～20% inoculum concentration, is fermented
The pH value of culture medium is 6.5, and wherein sucrose, bean cake addition is every liter of 40 grams of consumption, and other are inorganic salt, is led in sweat
Wind ratio is 0.9：1, temperature be 30 DEG C, dissolved oxygen for 25% fermentation culture obtain Bacillus licheniformis liquid bacterium number >=2.0 ×
109The fermentation culture of cfu/mL；
(4) fermentation culture is evaporated to into water content 50%～60% into double-effect evaporation tower, adds the wheat of 5wt%
Bud dextrin is spray-dried, and obtains viable count 20 × 109CFU/g activity Bacillus licheniformis powder.
3rd, enterococcus faecalis microbial inoculum is prepared
Enterococcus faecalis microbial inoculum is prepared in accordance with the following steps：
(1) activation culture：By enterococcus faecalis strain, the ring of picking 2 is inoculated in liquid seed culture medium from slant medium
In, 35 DEG C, 180r/min 3～5h of culture, (viable bacteria concentration is 10 to obtain seed liquor9CFU/mL activated seed liquid) is obtained；
(2) one-level spreads cultivation：The activated seed liquid is inoculated with first cell culture medium, 35 DEG C, 180r/min culture 6h are obtained
To primary seed solution；
(3) fermentation culture：The one-level culture fluid is inoculated with fermentation medium by 10～20% inoculum concentration, 37
DEG C, 100r/min, culture 12h obtains fermentation liquid, and fermentation liquid Jing 14000r/min centrifugation 30min obtain final product enterococcus faecalis active bacteria
Mud；
(4) enterococcus faecalis activity bacterium mud is carried out into expansion drying, obtains viable count 5 × 109The active enterococcus faecalis of CFU/g
Active bacteria powder.
Wherein：Slant medium mass component is consisted of：Glucose 2%, peptone 1%, yeast extract 0.5%, lemon
Lemon acid diammonium 0.2%, sodium acetate 0.5%, Carnis Bovis seu Bubali cream 1%, tween 80 0.1%, K2HPO40.2%, MgSO4·7H2O
0.0058%, MnSO4·4H2O 0.025%, agar powder 1.5%, balance of water, pH 7.0 ± 0.2；
Primary-seed medium mass component is consisted of：Sucrose 2.5%, soy peptone 1.8%, yeast extract
0.4%, MgSO4·7H2O 0.2%, MnSO4·4H2O 0.045%, NaCl 0.2%, dibasic ammonium citrate 0.2%, CaCO3
0.6%, balance of water, pH 7.0 ± 0.2；
Fermentation medium mass component is consisted of：Brown sugar 1.5%, soy peptone 0.5%, yeast extract 0.4%, sulfur
Sour magnesium 0.2%, manganese sulfate 0.045%, Sodium Chloride 0.2%, dibasic ammonium citrate 0.5%, Calcium Carbonate 0.1%, defoamer
0.005%, balance of water, pH 5.5-6.8.
4th, feedstuff Chinese medicine microecological preparation is prepared
Feedstuff Chinese medicine microecological preparation is prepared in accordance with the following steps：
(1) Chinese crude drug pretreatment
Chinese crude drug is chosen, is dried at 60 DEG C after 2.0h, crushed, cross 80 mesh sieves, standby, wherein Chinese medicine includes Huang
Stilbene, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum, are 10 by weight：10：3：6：9：5：
2：4 prepare；
(2) strain fermentating liquid is prepared
Aspergillus niger bacterium solution and trichodermaharzianum bacterium solution are prepared in accordance with the following steps：
By aspergillus niger carry out on PDA slant mediums 30 DEG C culture 72 hours after make spore suspension (8.0 × 108Individual/
ML), in being inoculated into one-level PDA culture fluid, 30 DEG C of cultivation temperature, pH value is 7.0, and 72 hours time, to logarithmic (log) phase, went to two grades
Identical culture is carried out in PDA culture fluid, to logarithmic (log) phase standby (5 × 109CFU/ml)；
By trichodermaharzianum carry out on PDA slant mediums 25 DEG C culture 120 hours after make spore suspension, be inoculated into reason
The primary seed solution of moral culture fluid is looked into, the second order fermentation of same culture medium prescription is transferred to when each bacteria growing is to exponential phase
Culture is enlarged in tank, stops cultivating when reaching exponential phase, standby (10 × 109CFU/ml)。
(3) herb fermenting
By the mix homogeneously according to a certain ratio of the Chinese crude drug after crushing, plus the water of equivalent weight, after immersion 24h, adjust pH value
Between 6.5～7.5, the steam sterilization 20min at 121 DEG C, after cooling, addition accounts for total strain liquid of Chinese crude drug gross weight 10%
(volume ratio of aspergillus niger bacterium solution and trichodermaharzianum bacterium solution is 1：1) 30 DEG C ferment 48 hours, stir once every 8h；Fermentation ends
Afterwards, dry at 40 DEG C 48 hours, 80 DEG C inactivate for two hours, standby；
In the present embodiment, the ratio of the Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid 15 ×
109CFU。
(4) formula feed Chinese medicine microecological preparation
Drying product in step (3) is crushed, 120 mesh sieves is crossed, with saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis
With mass ratio as 2：1：1：0.5(2g：10×109CFU：20×109CFU：2.5×109CFU) it is hybridly prepared into feedstuff Chinese medicine
Microbial ecological agent powder.
Granular preparation need to be such as made, the flavoring agent (sweet taste containing saccharin sodium is added in the mixed powder of aforementioned proportion
Agent) Traditional Chinese drug microecology granule system is made after 500mg/kg, antioxidant (ethoxy quinoline) 1000mg/kg and carrier (dextrin)
Agent, carrier (dextrin) content is 79.85%.200g containing Chinese medicine microecological preparation in every 1000g granular preparations after making.
Embodiment 2, prepare feedstuff Chinese medicine microecological preparation
First, Bradley yeast bacteria agent is prepared
With embodiment 1.
2nd, bacillus licheniformis agent is prepared
With embodiment 1.
3rd, enterococcus faecalis microbial inoculum is prepared
With embodiment 1.
4th, feedstuff Chinese medicine microecological preparation is prepared
Feedstuff Chinese medicine microecological preparation is prepared in accordance with the following steps：
(1) Chinese crude drug pretreatment
Chinese crude drug is chosen, is dried at 50 DEG C after 3.0h, crushed, cross 100 mesh sieves, standby, wherein Chinese medicine includes Huang
Stilbene, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum, are 3 by weight：3：8：9：3：10：4：
2 prepare；
(2) strain fermentating liquid is prepared
Aspergillus niger bacterium solution and trichodermaharzianum bacterium solution are prepared in accordance with the following steps：
By aspergillus niger carry out on PDA slant mediums 30 DEG C culture 72 hours after make spore suspension (8.0 × 108Individual/
ML), in being inoculated into one-level PDA culture fluid, 30 DEG C of cultivation temperature, pH value is 7.0, and 72 hours time, to logarithmic (log) phase, went to two grades
Identical culture is carried out in PDA culture fluid, to logarithmic (log) phase standby (5 × 109CFU/ml)；
By trichodermaharzianum carry out on PDA slant mediums 25 DEG C culture 120 hours after make spore suspension, be inoculated into reason
The primary seed solution of moral culture fluid is looked into, the second order fermentation of same culture medium prescription is transferred to when each bacteria growing is to exponential phase
Culture is enlarged in tank, stops cultivating when reaching exponential phase, standby (10 × 109CFU/ml)；
(3) herb fermenting
By the mix homogeneously according to a certain ratio of the Chinese crude drug after crushing, plus the water of equivalent weight, after immersion 24h, adjust pH value
Between 6.5～7.5, the steam sterilization 20min at 121 DEG C, after cooling, addition accounts for total strain liquid of Chinese crude drug gross weight 10%
(volume ratio of aspergillus niger bacterium solution and trichodermaharzianum bacterium solution is 1：1) 30 DEG C ferment 48 hours, stir once every 8h；Fermentation ends
Afterwards, dry at 40 DEG C 48 hours, 80 DEG C inactivate for two hours, standby；
In the present embodiment, the ratio of the Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid 15 ×
109CFU。
(4) formula feed Chinese medicine microecological preparation
Drying product in step (3) is crushed, 120 mesh sieves is crossed, with saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis
With mass ratio as 2.5：1：1：0.5(2.5g：10×109CFU：20×109CFU：2.5×109CFU) it is hybridly prepared into feedstuff use
Chinese medicine microecological preparation powder.
Granular preparation need to be such as made, the flavoring agent (sweet taste containing saccharin sodium is added in the mixed powder of aforementioned proportion
Agent) Traditional Chinese drug microecology granule system is made after 500mg/kg, antioxidant (ethoxy quinoline) 1000mg/kg and carrier (dextrin)
Agent, carrier (dextrin) content is 79.85%.200g containing Chinese medicine microecological preparation in every 1000g granular preparations after making.
Embodiment 3, prepare feedstuff Chinese medicine microecological preparation
First, the preparation of Bradley yeast bacteria agent
With embodiment 1.
2nd, bacillus licheniformis agent is prepared
With embodiment 1.
3rd, enterococcus faecalis microbial inoculum is prepared
With embodiment 1.
4th, feedstuff Chinese medicine microecological preparation is prepared
Feedstuff Chinese medicine microecological preparation is prepared in accordance with the following steps：
(1) Chinese crude drug pretreatment
Chinese crude drug is chosen, is dried at 60 DEG C after 2.0h, crushed, cross 80 mesh sieves, standby, wherein Chinese medicine includes Huang
Stilbene, the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, Radix Bupleuri, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum, are 5 by weight：5：5：3：6：2：3：3
Prepare；
(2) strain fermentating liquid is prepared
Aspergillus niger bacterium solution and trichodermaharzianum bacterium solution are prepared in accordance with the following steps：
By aspergillus niger carry out on PDA slant mediums 30 DEG C culture 72 hours after make spore suspension (8.0 × 108Individual/
ML), in being inoculated into one-level PDA culture fluid, 30 DEG C of cultivation temperature, pH value is 7.0, and 72 hours time, to logarithmic (log) phase, went to two grades
Identical culture is carried out in PDA culture fluid, to logarithmic (log) phase standby (5 × 109CFU/ml)；
By trichodermaharzianum carry out on PDA slant mediums 25 DEG C culture 120 hours after make spore suspension, be inoculated into reason
The primary seed solution of moral culture fluid is looked into, the second order fermentation of same culture medium prescription is transferred to when each bacteria growing is to exponential phase
Culture is enlarged in tank, stops cultivating when reaching exponential phase, standby (10 × 109CFU/ml)；
(3) herb fermenting
By the mix homogeneously according to a certain ratio of the Chinese crude drug after crushing, plus the water of equivalent weight, after 12～24h of immersion, adjust
Between pH value 6.5～7.5, the steam sterilization 20min at 121 DEG C, after cooling, addition accounts for total strain of Chinese crude drug gross weight 20%
(volume ratio of aspergillus niger bacterium solution and trichodermaharzianum bacterium solution is 1 to liquid：1) 28 DEG C ferment 60 hours, stir once every 8h；Fermentation knot
Shu Hou, dries 48 hours at 50 DEG C, and 80 DEG C inactivate for two hours, standby；
In the present embodiment, the ratio of the Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid 15 ×
109CFU。
(4) formula feed Chinese medicine microecological preparation
Drying product in step (3) is crushed, 120 mesh sieves is crossed, with saccharomyces boulardii, Bacillus licheniformis and enterococcus faecalis
With mass ratio as 1.5：1：0.5：0.5(1.5g：10×109CFU：10×109CFU：2.5×109CFU) it is hybridly prepared into feedstuff
Use Chinese medicine microecological preparation powder；
Granular preparation need to be such as made, the flavoring agent (sweet taste containing saccharin sodium is added in the mixed powder of aforementioned proportion
Agent) Traditional Chinese drug microecology granule system is made after 500mg/kg, antioxidant (ethoxy quinoline) 1000mg/kg and carrier (dextrin)
Agent, carrier (dextrin) content is 79.85%.200g containing Chinese medicine microecological preparation in every 1000g granular preparations after making.
The application of embodiment 4, feedstuff Chinese medicine microecological preparation in poultry feed intake is improved
Feedstuff is with Chinese medicine microecological preparation as addition during additive for farm animal feed typically in 700～3000g/t, feedstuff
For the conventional special feed suitable for each poultry without antibiotic；Addition in sow feed is 1000～2000g/t；
Addition in young sow feed is educated is 1500～3000g/t；Addition in fattening pannage is 700～1500g/t.
With the sow before and after farrowing as experimental subject, the genetic background sow 40 consistent with pregnancy time is chosen, averagely
Be divided into two groups, respectively experimental group and matched group, 20 per group, experimental group from antenatal 1 month to weaned piglet during press
Addition addition feedstuff Chinese medicine microecological preparation (prepared by the embodiment 1) feeding of 1000g/t, control group fed normal diet,
It is about 55 days experimental period.Experimental result is：The per day feed intake of sow of experimental group improves 13.6wt% than matched group, young
Pig birth counterpoise experimental group improves 13.9wt% than matched group, and piglet counterpoise experimental group improves than matched group during wean
22wt%.
With the piglet after wean as experimental subject, 25 days ablactational baby pig 60 that genetic background is identical, body weight is close are chosen,
It is divided into two groups, respectively experimental group and matched group, 30 per group, experimental group is pressed the addition addition feedstuff of 1200g/t and used
Chinese medicine microecological preparation (prepared by embodiment 1) feeds, control group fed normal diet, and experimental period is 4 weeks.Experimental result is：
The per day feed intake experimental group of piglet improves 20.4wt% than matched group, and the per day weightening experimental group of piglet is improved than matched group
28.3wt%, piglet diarrhea rate experimental group reduces 58.8wt% than matched group, observation experiment group piglet fur during test
More smooth, disposition is active.
Above contrast test proves that the present invention can significantly improve the feed intake of animal, strengthens animal physique, promotes animal
Growth.
Embodiment 5, feedstuff improves the application in livestock and poultry production performance and immune performance with Chinese medicine microecological preparation
First, white meat-type chickens
From 2000 1 age in days healthy AA white meat-type chickens, weigh after 43 days, be randomly divided into 2 experimental grouies, each experimental group
If 5 repetitions, each repeats 200 chickens, and male and female respectively accounts for 1/2.Test group feedstuff is respectively：(1) this is added in test group feedstuff
Invention feedstuff Chinese medicine microecological preparation (prepared by embodiment 2) 1500g/t；(2) matched group is normal diet.Test group and control
Group feed formula as scale of feeding, test chicken free choice feeding and drinking-water, feeding and management and immune programme for children are with reference to feeding of broiler pipe
Reason handbook.Experimental result is as shown in table 1.
Impact of the table 1, chicken feed probiotics to meat chicken growth performance
In table 1：Same column letter is different to represent significant difference (P ＜ 0.05).
This result of the test shows that feedstuff Chinese medicine microecological preparation of the present invention significantly improves the production performance of white meat-type chickens
And immune performance, compared with matched group, feedstuff-meat ratio reduces by 35.8%, and death rate reduces by 56%.
2nd, Cherry Village Duckss
1 age in days Cherry Village Duckss 4000, matched group 2000, test group 2000, with basal diet are selected in this test
As a control group, without feedstuff Chinese medicine microecological preparation, test group is the addition feedstuff Chinese medicine on the basis of basal diet
Microbial ecological agent (prepared by embodiment 2) 1500g/t, totally 2 treatment groups.Test is divided into early stage (1-21 ages in days) and later stage (22-42
Age in days) two stages.Using net-rearing, special messenger raising, free choice feeding, drinking-water, gravity-flow ventilation routinely immune programme for children immunity.
This result of the test shows that test group is respectively increased the age in days of meat-type duck 0～21,22～42 ages in days, 1-42 age in days meat-type duck days
Weightening 7.60%, 4.04%, 5.35%, reduces respectively feedstuff-meat ratio 0.09,0.2,0.17 (P ＜ 0.05).The feedstuff micro- life of Chinese medicine
State preparation group significantly improves the daily gain (P ＜ 0.05) of Cherry Village Duckss early, late and full, significantly reduces each stage material meat
Than (P ＜ 0.05).This test shows that adding feedstuff Chinese medicine microecological preparation in Cherry Village Duckss feedstuff can significantly improve
The immunity and growth performance of meat-type duck, reduces meat-type duck mortality rate.
3rd, milch cow
The milch cow 30 of experimental selection 2-5 in puerperal month, takes according to parity, lactogenic monthly age, body condition, body weight, milk yield
Matching method is classified as matched group and experimental group, and 2 groups of differences are not notable.Experimental group feeding contains the micro- life of Chinese medicine of this feedstuff
The feedstuff of state preparation (prepared by embodiment 3), its addition is 1000g/t, and matched group feeds normal diet, and experimental period is 5 weeks.It is real
Test result to show：During testing, without large change, experimental group averagely per increases daily 2.70 kilograms to matched group production capacity milk amount
The output of fresh milk, increases by 9.49%, and to experiment at the end of, the average every daily yielding of experimental group is fresh compared with increasing by 5.4 kilograms before testing
Milk, increases by 19.45%, and the sickness rate of mastitis is greatly lowered, and difference is extremely notable.Illustrate that the present invention can be effectively increased milk
The immunity and production capacity of cattle.
It should be understood that these embodiments are only illustrative of the invention and is not intended to limit the scope of the invention.In addition, it is to be understood that
After the content for having read instruction of the present invention, those skilled in the art can make various changes or modifications to the present invention, these
The equivalent form of value equally falls within the application appended claims limited range.
Claims (10)
1. a kind of feedstuff Chinese medicine microecological preparation, it is characterised in that：It includes (1.5～2.5) g：(5×109～15 × 109)
CFU：(10×109～30 × 109)CFU：(0.5×109～2.5 × 109) the herb fermenting extract of CFU, saccharomyces boulardii,
Bacillus licheniformis and enterococcus faecalis；The herb fermenting extract is by Chinese crude drug inoculated aspergillus niger and trichodermaharzianum fermentation
Into preparation process is as follows：Mix after each component drying, crushing that the Chinese crude drug will be constituted, sterilize after soaking, access black
The mixed bacteria liquid of aspergillosis and trichodermaharzianum, is obtained after fermented, drying and inactivation；The Chinese crude drug is (1～10) by mass ratio：
(1～10)：(1～10)：(1～10)：(1～10)：(1～10)：(1～5)：The Radix Astragali of (1～5), the Cortex Eucommiae, Radix Glycyrrhizae, Radix Angelicae Sinensis, bavin
Recklessly, Herba Patriniae, Herba portulacae, Rhizoma Picrorhizae and Halloysitum Rubrum composition.
2. feedstuff Chinese medicine microecological preparation according to claim 1, it is characterised in that：The aspergillus niger and trichodermaharzianum
Mixed bacteria liquid in, the concentration of aspergillus niger is (2.5 × 109～15 × 109)CFU/mL；The concentration of trichodermaharzianum is (5 × 109~
20×109)CFU/mL。
3. feedstuff Chinese medicine microecological preparation according to claim 1 and 2, it is characterised in that：In the fermentation step, institute
The ratio for stating Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid (10 × 109～35 × 109)CFU；And/or,
The temperature of the fermentation is 28～37 DEG C, and the time is 24～72 hours.
4. the feedstuff Chinese medicine microecological preparation according to any one of claim 1-3, it is characterised in that：The feedstuff is used
Chinese medicine microecological preparation also includes pharmaceutic adjuvant；In the Chinese medicine microecological preparation, the weight/mass percentage composition of the pharmaceutic adjuvant
For 50%～98%.
5. the preparation method of the feedstuff Chinese medicine microecological preparation any one of claim 1-4, comprises the steps：
(1) each component of the Chinese crude drug will be constituted successively after dried, crushed according to mass ratio mixing, Chinese crude drug is obtained；
(2) will be soaked in water in the Chinese crude drug, obtain immersion system；Inoculated aspergillus niger and healthy and free from worry after the immersion system is sterilized
The mixed bacteria liquid of trichoderma, after fermented, drying and inactivation herb fermenting extract is obtained；
(3) by the fermented product extract, the saccharomyces boulardii, the Bacillus licheniformis and the enterococcus faecalis according to described
Ratio mixes, you can obtain the feedstuff Chinese medicine microecological preparation.
6. preparation method according to claim 5, it is characterised in that：In step (1), the temperature of the drying is 50～60
DEG C, the time is 2～3h；The mesh number of the crushing is 80～120 mesh.
7. the preparation method according to claim 5 or 6, it is characterised in that：It is described in the soaking step in step (2)
Chinese crude drug is 1 with the mass ratio of the water：(1～1.5), the temperature of immersion is 15～25 DEG C, and the time is 12～24 hours；It is described
Also include the step of pH value of soak being adjusted between 6.5～7.5 after immersion.
8. the preparation method according to any one of claim 5-7, it is characterised in that：In step (2), the aspergillus niger and
In the mixed bacteria liquid of trichodermaharzianum, the concentration of aspergillus niger is (2.5 × 109～15 × 109) CFU/mL, the concentration of trichodermaharzianum is
(5×109～20 × 109)CFU/mL；The ratio of the Chinese crude drug and the mixed bacteria liquid is Chinese crude drug 100g：Mixed bacteria liquid (10
×109～35 × 109)CFU；And/or, the temperature of the fermentation is 28～37 DEG C, and the time is 24～72 hours.
9. the preparation method according to any one of claim 5-8, it is characterised in that：In step (2), the baking step
In, drying temperature is 35～50 DEG C, and the time is 24～48 hours.
10. the feedstuff Chinese medicine microecological preparation any one of claim 1-4 is following 1) -8) at least one of in
Using：
1) poultry feed intake is improved；
2) poultry daily gain is improved；
3) poultry body constitution is strengthened；
4) growth of animals or poultry is promoted；
5) livestock and poultry production performance is improved；
6) fowl poultry immune performance is improved；
7) poultry death rate is reduced；
8) poultry feedstuff-meat ratio is reduced.
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| CN110495522A (en) * | 2019-07-19 | 2019-11-26 | 湖北大学 | A kind of feed Chinese medicine microecological preparation |
| CN110495522B (en) * | 2019-07-19 | 2022-04-12 | 湖北大学 | Traditional Chinese medicine microecological preparation for feed |
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