CN106962643A - A kind of production method of nonreactive grower pigs fermented feed - Google Patents
A kind of production method of nonreactive grower pigs fermented feed Download PDFInfo
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- CN106962643A CN106962643A CN201710220838.5A CN201710220838A CN106962643A CN 106962643 A CN106962643 A CN 106962643A CN 201710220838 A CN201710220838 A CN 201710220838A CN 106962643 A CN106962643 A CN 106962643A
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- 241000282887 Suidae Species 0.000 title claims abstract description 59
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000012010 growth Effects 0.000 claims abstract description 13
- 235000014590 basal diet Nutrition 0.000 claims abstract description 10
- 239000003205 fragrance Substances 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 30
- 239000002609 medium Substances 0.000 claims description 29
- 239000002054 inoculum Substances 0.000 claims description 23
- 241000894006 Bacteria Species 0.000 claims description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 17
- 239000004310 lactic acid Substances 0.000 claims description 15
- 235000014655 lactic acid Nutrition 0.000 claims description 15
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 241000194032 Enterococcus faecalis Species 0.000 claims description 8
- 241000186660 Lactobacillus Species 0.000 claims description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 8
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 8
- 240000001929 Lactobacillus brevis Species 0.000 claims description 8
- 235000013957 Lactobacillus brevis Nutrition 0.000 claims description 8
- 244000199866 Lactobacillus casei Species 0.000 claims description 8
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 8
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 8
- 229940039696 lactobacillus Drugs 0.000 claims description 8
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 8
- 229940017800 lactobacillus casei Drugs 0.000 claims description 8
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 7
- 239000008101 lactose Substances 0.000 claims description 7
- 235000012054 meals Nutrition 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 235000019733 Fish meal Nutrition 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 239000004467 fishmeal Substances 0.000 claims description 6
- 244000025254 Cannabis sativa Species 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 230000008676 import Effects 0.000 claims description 5
- 229960001375 lactose Drugs 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- 241000726221 Gemma Species 0.000 claims description 4
- 238000011218 seed culture Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 239000012879 subculture medium Substances 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 abstract description 3
- 229940088710 antibiotic agent Drugs 0.000 abstract description 3
- 235000005911 diet Nutrition 0.000 abstract description 2
- 230000037213 diet Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 19
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 18
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 14
- 239000001888 Peptone Substances 0.000 description 14
- 108010080698 Peptones Proteins 0.000 description 14
- 239000012153 distilled water Substances 0.000 description 14
- 239000008103 glucose Substances 0.000 description 14
- 235000019319 peptone Nutrition 0.000 description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 12
- 235000015278 beef Nutrition 0.000 description 12
- 230000008901 benefit Effects 0.000 description 12
- 229940041514 candida albicans extract Drugs 0.000 description 12
- 239000000284 extract Substances 0.000 description 12
- 239000012138 yeast extract Substances 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 11
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 10
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 10
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 10
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 10
- 229920000053 polysorbate 80 Polymers 0.000 description 10
- 239000001632 sodium acetate Substances 0.000 description 10
- 235000017281 sodium acetate Nutrition 0.000 description 10
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 239000004202 carbamide Substances 0.000 description 6
- 229910052564 epsomite Inorganic materials 0.000 description 6
- 235000013379 molasses Nutrition 0.000 description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 5
- 235000011130 ammonium sulphate Nutrition 0.000 description 5
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 5
- 239000007836 KH2PO4 Substances 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
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- 238000005516 engineering process Methods 0.000 description 3
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- 230000035764 nutrition Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
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- 238000009360 aquaculture Methods 0.000 description 2
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- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
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- 150000007524 organic acids Chemical class 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
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- 230000000593 degrading effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/121—Brevis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Biomedical Technology (AREA)
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- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of production method of nonreactive grower pigs fermented feed.Described nonreactive grower pigs fermented feed is characterised by, including below according to the raw material of mass percent meter：Microbe leaven 1.0% -2.0%, growth pig feed 42% -71% and water 28% -56%.Described production method is：Above-mentioned nonreactive grower pigs fermented feed raw material is mixed evenly, compacting vacuumizes sealing after pack, and in 26-35 DEG C of 48-72h of anaerobic fermentation, pH value drops to less than 4.4, and has strong sour fragrance, you can adds and uses in full price basal diet.20% -50% adequate diet is substituted in Large-scale pig farm with the nonreactive grower pigs fermented feed of the present invention to be fed; feeding cost can be reduced, effectively alleviate grower pigs stress, significantly improve immune performance and growth performance; the use of simultaneously substitute antibiotics is reduced, so as to improve fanning economics.
Description
Technical field
The invention belongs to livestock breeding industry field, and in particular to a kind of production method of nonreactive grower pigs fermented feed.
Background technology
With the continuous improvement of people's living standards, increasingly paying attention to food quality and safety, to high-quality meat product
Requirement also more and more higher, healthy aquaculture is increasingly becoming the common recognition of people.Traditional livestock breeding industry has abuse of antibiotics
And medicament residue problem, not only cultivated animals are caused with huge harm, while also being constituted to human health and safety huge
Threat, do not met modern sustainable development and the requirement of healthy aquaculture.Countries in the world are made laws in succession in recent years limits anti-
Use of the raw element in feed, the food-safe and healthy attention of people reaches unprecedented height.Therefore, live pig is carried out
Nonreactive is cultivated, and reduces the use of antibiotic and chemicals, and improving breeding environment and improving consumption safety turns into trend of the times.
In nonreactive breeding process, how to reduce pig, stress improve the key that production performance is large-scale cultivation.Pass through
Feed formula reasonable in design, biological fermentation feed is made full use of to be the scientific and reasonable way for realizing feed nonreactive or even nonreactive cultivation
One of footpath.
Because all feeds price rose steadily in recent years, feed resource is short, new alternate resources and nutrient formulation
Research turn into industrial hot spot.Palm kernel meal is the fruit of palm, is the torrid areas yield excellent honest and clean dregs of rice class original of price greatly
Material, is a kind of effective way of reduction pannage cost to its reasonable utilizes.
Biological fermentation feed is that microbial fermentation technology is the animal feed or feedstuff that core is produced, at fermentation
The feed of reason can not only change feed palatability, moreover it is possible to toxin and ANFs that may be present in feedstuff of degrading, and carry
High nutrition absorption level simultaneously improves animal body health, so as to improve the security of livestock products.Containing big in biological fermentation feed
The beneficial microbe of amount plays key effect in animal immune performance and production performance is improved, but the selection of its efficient bacterium and
Standardized production is also one the problem of be worth going into seriously.
The content of the invention
It is an object of the invention to provide a kind of production method of nonreactive grower pigs fermentation material, by using the original of super quality and competitive price
Expect and utilize microbial fermentation technology that Reasonable Regulation And Control dietary nutritional standard effectively solves in breeding process the epidemic disease that occurs and low
Efficiency problem, improves the quality and fanning economics of livestock products.
To realize object above, the invention provides a kind of production method of nonreactive grower pigs fermented feed, it is without antibiosis
Long pig fermented feed raw material by mass percentage, including microbe leaven 1.0% -2.0%, growth pig feed 42% -71% and
Water 28% -56%.
In nonreactive grower pigs fermented feed raw material, described microbe leaven is by mixing lactic acid bacteria, saccharomyces cerevisiae
Bacterium and bacillus subtilis, expand after numerous culture respectively through secondary seed, according to mass ratio 3：1：1 mixing is produced.
In microbe leaven, described mixing lactic acid bacteria, S. cervisiae and bacillus subtilis, respectively two step
Prepare：
Step 1：The preparation of first order seed
By lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, lactose lactobacillus, S. cervisiae and withered grass gemma
Bacillus, respectively preferred optimal primary-seed medium and under the conditions of cultivate, obtain corresponding first order seed；
Step 2：The preparation of secondary seed
（1）By the lactobacillus acidophilus obtained in the step 1, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, lactose lactobacillus
First order seed in mass ratio 2：2：2：1：1, inoculum concentration is 10%, and combined inoculation is in lactic acid bacteria secondary seed medium, 30-37
48-72h of quiescent culture under the conditions of DEG C, viable count reaches 1010Cfu/mL, produces mixing lactic acid bacteria secondary seed；
（2）By the S. cervisiae first order seed obtained in the step 1, by inoculum concentration 8%, secondary seed medium is inoculated in,
9-12h is cultivated under the conditions of 30-35 DEG C, 160-210r/min of rotating speed.Viable count reaches 108 Cfu/mL, produces saccharomyces cerevisiae
Bacterium secondary seed；
（3）By the bacillus subtilis first order seed obtained in the step 1, by inoculum concentration 8%, secondary seed culture is inoculated in
Base, cultivates 18-24h under the conditions of 35-43 DEG C, 180-240r/min of rotating speed, and viable count reaches 1010 Cfu/mL, is produced withered
Careless bacillus secondary seed.
It is preferred that, the lactobacillus acidophilus first order seed optimal medium of the step 1：Peptone 10g, yeast extract
6g, beef extract 12g, glucose 16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2The g of O 0.58,
MnSO4·4H2O 0.05g, K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration
1.0%, culture medium initial ph value is 5.5.
It is preferred that, the Lactobacillus brevis first order seed optimal medium of the step 1：Peptone 12g, yeast extract 5g,
Beef extract 12g, glucose 16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2The g of O 0.58,
MnSO4·4H2O 0.05g, K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration
For 1.0%, culture medium initial ph value is 5.5.
It is preferred that, the Lactobacillus casei first order seed optimal medium of the step 1：Peptone 12g, yeast extract
6g, beef extract 6g, glucose 18g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2The g of O 0.58,
MnSO4·4H2O 0.05g, K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration
1.0%, culture medium initial ph value is 6.5.
It is preferred that, the enterococcus faecalis first order seed optimal medium of the step 1：Peptone 12g, yeast extract 6g,
Beef extract 10g, glucose 22g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2The g of O 0.58,
MnSO4·4H2O 0.05g, K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 48h, inoculum concentration
2.0%, culture medium initial ph value 8.5.
It is preferred that, the lactose lactobacillus first order seed optimal medium of the step 1：Peptone 8g, yeast extract 6g,
Beef extract 8g, glucose 16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2The g of O 0.58,
MnSO4·4H2O 0.05g, K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 48h, inoculum concentration
2.0%, culture medium initial ph value is 9.0.
It is preferred that, the S. cervisiae first order seed of the step 1, optimal medium：Glucose 25g, peptone 25g,
Yeast extract 15g, MgSO4·7H2O 5.0 g, KH2PO43.0g, distilled water 1000mL.Optimal culture condition：33 DEG C of temperature,
Time is 9h, inoculum concentration 1.2%, culture medium initial ph value 4.2.
It is preferred that, the bacillus subtilis first order seed optimal medium of the step 1：Glucose 3.8g, peptone
3.8g, beef extract 3.8g, starch 2.3g, NaCI 3.3g, KH2PO4 3.3g, distilled water 1000mL.Optimal culture condition：Temperature
42 DEG C, time 18h, inoculum concentration 1.5%, culture medium starting pH is 8.0.
It is preferred that, the mixing lactic acid bacteria secondary seed medium proportioning of the step 2：Concentration of molasses is 16 ° of BX, urea
0.10%, ammonium sulfate 0.10%, magnesium sulfate 0.04%, potassium dihydrogen phosphate 0.15%, initial ph value is 5.5-6.0.
It is preferred that, the saccharomyces cerevisiae secondary seed medium proportioning of the step 2：10 ° of Bx of concentration of molasses, ammonium sulfate
0.10%, urea 0.10%, potassium dihydrogen phosphate 0.16%, epsom salt 0.05%, culture medium initial ph value is 4.2-5.0.
It is preferred that, the bacillus subtilis secondary seed medium proportioning of the step 2：6.0 ° of BX of concentration of molasses, sulfuric acid
Ammonium 0.2%, urea 0.15%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.4%, culture medium initial ph value is 8.0.
In the nonreactive grower pigs feedstuff, growth pig feed includes following materials in percentage by mass：Corn 53-
70%, dregs of beans 17-27%, palm kernel meal 3-10%, two grades of fish meal 0-3% of import, wheat bran 4-8%, soya-bean oil 0-2%, mould inhibitor
0.1%, premix 4%.
The production method of the nonreactive grower pigs fermented feed, nonreactive grower pigs fermented feed is constituted according to raw material and mixed
Stir, compacting vacuumizes sealing after pack, in 26-35 DEG C of 48-72h of sealed fermenting, and pH value drops to less than 4.4, and
There is strong sour fragrance, you can add and use in the full price basal diet of grower pigs.
In the application method of the nonreactive grower pigs fermented feed, product of the present invention adds in grower pigs in full price basal diet
Dosage is 20% -50%.
The invention has the advantages that and advantage
（1）The nonreactive grower pigs fermented feed of the present invention, is the nutritional need according to pig growth phase, peculiar from torrid areas
Palm kernel meal and high-grade maize, dregs of beans and fish meal, by improving feed formula, Reasonable Regulation And Control nutriment ratio, it can be
Growth phase pig provides suitable and abundant nutrition, the growth potential of pig is given full play to, without any antibiotic；
（2）The nonreactive grower pigs fermented feed of the present invention, the microbe leaven activity used is high, and the fermentation of nonreactive grower pigs is raised
Living bacteria count amount is up to 10 in material7-108Cfu/g, can secrete substantial amounts of organic acid, digestive ferment and benefit materials, promote nutrition
Digesting and assimilating for material, improves intestinal health.Nutriment structure and content make moderate progress in nonreactive grower pigs fermented feed, have
Beneficial to improving feed palatability and improve feed digestion utilization rate；
（3）Present invention optimizes in production nonreactive grower pigs fermented feed starter culture with when adding proportion, in scale
Change nonreactive grower pigs fermented feed of the pig farm using the present invention, it is possible to increase the feed intake of grower pigs, reduce the incidence of disease, significantly
Immune performance and growth performance are improved, so that the effect for reducing simultaneously substitute antibiotics is played, while also improving culturing economic effect
Benefit；
（4）The standardized production of nonreactive grower pigs fermented feed of the present invention is in optimization composite bacteria condition of culture and use ratio
Under the premise of screen on the basis of influence to pig average daily gain, the incidence of disease and economic benefit, therefore the scientific formulation
Rationally, it is with a wide range of applications；
（5）The nonreactive grower pigs fermented feed of the present invention uses raw material sources extensively, cheap, the letter of production operation specification
Just, without complicated and cumbersome process treatment process, feed and easy to use, can be raised for the development and fermentation of nonreactive cultural technique
Efficient utilize of material provides effective way, and provides technical support for the scale of live pig, intensive manufacture.
Embodiment
The present invention is further described with reference to example, it is micro- described in following examples for the ease of narration
Bio-fermentation agent is by mixing lactic acid bacteria, S. cervisiae and bacillus subtilis secondary seed culture, according to mass ratio 3：
1：1 is mixed to get, and its total viable count is up to 1010More than cfu/mL；Grower pigs Hu full price basal diet herds former industry by Hainan green grass or young crops to be had
Limit company produces.
Microbe leaven preparation method is obtained suddenly in two steps：
Step 1：The preparation of first order seed
By lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, lactose lactobacillus, S. cervisiae and withered grass gemma
Bacillus, respectively preferred optimal primary-seed medium and under the conditions of cultivate, obtain corresponding first order seed.
Step 2：The preparation of secondary seed
（1）By the lactobacillus acidophilus obtained in step 1, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, lactose lactobacillus one-level
Seed in mass ratio 2：2：2：1：1, inoculum concentration is 10%, and combined inoculation is in lactic acid bacteria secondary seed medium, in 30-37 DEG C of bars
48-72h of quiescent culture under part, viable count reaches 1010Cfu/mL, produces mixing lactic acid bacteria secondary seed；
（2）By the S. cervisiae first order seed obtained in step 1, by inoculum concentration 8%, secondary seed medium is inoculated in,
30-35 DEG C, 9-12h is cultivated under the conditions of 160-210r/min of rotating speed.Viable count reaches 108Cfu/mL, produces saccharomyces cerevisiae
Secondary seed；
（3）By the bacillus subtilis first order seed obtained in step 1, by inoculum concentration 8%, secondary seed medium is inoculated in,
35-43 DEG C, 18-24h is cultivated under the conditions of 180-240r/min of rotating speed, viable count reaches 1010Cfu/mL, produces withered grass gemma
Bacillus secondary seed.
Lactobacillus acidophilus first order seed optimal medium：Peptone 10g, yeast extract 6g, beef extract 12g, glucose
16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05g,
K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration 1.0%, culture medium initial ph value
For 5.5.
Lactobacillus brevis first order seed optimal medium：Peptone 12g, yeast extract 5g, beef extract 12g, glucose
16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05g,
K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration is 1.0%, culture medium starting pH
It is worth for 5.5.
Lactobacillus casei first order seed optimal medium：Peptone 12g, yeast extract 6g, beef extract 6g, glucose
18g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05g,
K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 24h, inoculum concentration 1.0%, culture medium initial ph value
For 6.5.
Enterococcus faecalis first order seed optimal medium：Peptone 12g, yeast extract 6g, beef extract 10g, glucose
22g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05g,
K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 48h, inoculum concentration 2.0%, culture medium starting pH
Value 8.5.
Lactose lactobacillus first order seed optimal medium：Peptone 8g, yeast extract 6g, beef extract 8g, glucose
16g, sodium acetate 5g, ammonium citrate 2.15g, Tween80 1mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05g,
K2HPO42g, distilled water 1000mL；Optimal culture condition：35 DEG C of temperature, time 48h, inoculum concentration 2.0%, culture medium initial ph value
For 9.0.
S. cervisiae first order seed, optimal medium：Glucose 25g, peptone 25g, yeast extract 15g, MgSO4·
7H2O 5.0 g, KH2PO43.0g, distilled water 1000mL.Optimal culture condition：33 DEG C of temperature, the time is 9h, inoculum concentration 1.2%,
Culture medium initial ph value 4.2.
Bacillus subtilis first order seed optimal medium：Glucose 3.8g, peptone 3.8g, beef extract 3.8g, starch
2.3g, NaCI 3.3g, KH2PO4 3.3g, distilled water 1000mL.Optimal culture condition：42 DEG C of temperature, time 18h, inoculum concentration
1.5%, culture medium starting pH is 8.0.
Mixing lactic acid bacteria secondary seed medium is matched：Concentration of molasses is 16 ° of BX, urea 0.10%, ammonium sulfate 0.10%, sulphur
Sour magnesium 0.04%, potassium dihydrogen phosphate 0.15%, initial ph value is 5.5-6.0.
Saccharomyces cerevisiae secondary seed medium is matched：10 ° of Bx of concentration of molasses, ammonium sulfate 0.10%, urea 0.10%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.16%, epsom salt 0.05%, culture medium initial ph value is 4.2-5.0.
Bacillus subtilis secondary seed medium is matched：6.0 ° of BX of concentration of molasses, ammonium sulfate 0.2%, urea 0.15%,
Potassium dihydrogen phosphate 0.2%, sodium chloride 0.4%, culture medium initial ph value is 8.0.
1st, embodiment 1
The production method of nonreactive grower pigs fermented feed of the present invention is such as：
Raw material in nonreactive grower pigs fermented feed weighs mixing by following mass percent：Microbe leaven 2.0%, growth
Pig feed 48% and water 50%.
Complete feed used in nonreactive grower pigs fermented feed, including following materials in percentage by mass：Corn 62.15%, beans
The dregs of rice 23%, palm kernel meal 5%, two grades of fish meal 1% of import, wheat bran 4%, soya-bean oil 0.75%, mould inhibitor 0.1%, premix 4%.
Above-mentioned nonreactive grower pigs fermented feed raw material is mixed evenly, compacting vacuumizes sealing after pack, in 26.4
After DEG C sealed fermenting 72h, pH value drops to 4.2, and has strong sour fragrance.
2nd, embodiment 2
Nonreactive of the present invention grows the production method of fermented feed：
Raw material in nonreactive grower pigs fermented feed weighs mixing by following mass percent：Microbe leaven 1.5%, growth
Pig feed 43.5% and water 55%.
Complete feed used in nonreactive grower pigs fermented feed, including following materials in percentage by mass：Corn 56.15%, beans
The dregs of rice 25%, palm kernel meal 6%, two grades of fish meal 2% of import, wheat bran 5%, soya-bean oil 1.75%, mould inhibitor 0.1%, premix 4%.
Above-mentioned nonreactive grower pigs fermented feed raw material is mixed evenly, compacting vacuumizes sealing after pack, in 30.1
DEG C sealed fermenting 48h, pH value drops to 4.4, and has strong sour fragrance.
3rd, embodiment 3
The production method of nonreactive grower pigs fermented feed of the present invention：
Raw material in nonreactive grower pigs fermented feed weighs mixing by following mass percent：Microbe leaven 1.0%, growth
Pig feed 61% and water 38%.
Complete feed used in nonreactive grower pigs fermented feed, including following materials in percentage by mass：Corn 58%, dregs of beans
26.15%, palm kernel meal 4%, two grades of fish meal 0.5% of import, wheat bran 7%, soya-bean oil 0.25%, mould inhibitor 0.1%, premix 4%.
Above-mentioned nonreactive grower pigs fermented feed raw material is mixed evenly, compacting vacuumizes sealing after pack, in 29.8
DEG C sealed fermenting 60h, pH value drops to 4.2, and has strong sour fragrance.
In the nonreactive grower pigs fermented feed product obtained by the fermentation of above-described embodiment 1-3, nutritive index crude protein
Matter, crude fat, coarse ash, calcium, total phosphorus and content of inorganic phosphorus increase, and are decreased before crude fiber content ratio fermentation,
The content of organic acid lactic acid, acetic acid and propionic acid is then significantly improved, and feed quality makes moderate progress, can be to immune system and animal health
Produce actively impact.
4th, test example 1
This test example is to grower pigs（50—75kg）Feed different proportion nonreactive grower pigs fermented feed and carry out cultivation contrast test.
Nonreactive grower pigs fermented feed is prepared according to the method for above-described embodiment 1.From three way cross (Du × length × big) pig
448,4 groups are randomly divided into by sex and body weight, each treatment group pig male and female half and half, original body mass is without significant difference.Same
Under feeding and management condition, control group fed grower pigs full price basal diet, experiment 1,2,3 groups add respectively in full price basal diet
Plus 20%, 30% and 50%（Calculated with air-drying sample）Nonreactive grower pigs fermented feed, with half wet condition feed.Determine daily gain, adopt
The growth performances such as appetite, feedstuff-meat ratio and economic benefit index of correlation.
Table 1 feeds different proportion nonreactive grower pigs fermented feed to grower pigs（50—75kg）Production performance and economic effect
The influence of benefit
In upper table, the identical lowercase letter indication difference of colleague's data shoulder mark not significantly (P>0.05), different lowercase letters are poor
It is different significantly (P<0.05)。
As known from Table 1, relative to control group, 3 test group heads daily ingestion amount, average head daily gain are improved, feed
Remuneration has larger improvement, and feedstuff-meat ratio is substantially reduced, with test 3 groups of effects preferably, with control group in significant difference (P<0.05).Examination
The morbidity head for testing group is below than control group, therefore, the medicine less investment of test group pig.Come from final profit result
See, the economic benefit that test group is obtained also is above control group.
5th, test example 2
This test example is to grower pigs（25—50kg）Feed different proportion nonreactive grower pigs fermented feed and carry out cultivation contrast test.
Nonreactive grower pigs fermented feed is prepared according to the method for above-described embodiment 2.From three way cross (Du × length × big) pig
416,4 groups are randomly divided into by sex and body weight, each treatment group pig male and female half and half, original body mass is without significant difference.Same
Under feeding and management condition, control group fed grower pigs full price basal diet, experiment 1,2,3 groups add respectively in full price basal diet
Plus 20%, 30% and 50%（Calculated with air-drying sample）Nonreactive grower pigs fermented feed, with half wet condition feed.Determine daily gain, adopt
The growth performances such as appetite, feedstuff-meat ratio and economic benefit index of correlation.
Table 2 feeds different proportion nonreactive grower pigs fermented feed to grower pigs（25—50kg）Production performance and economic benefit
Influence
In upper table, the different capitalizations of colleague's data shoulder mark represent difference extremely significantly (P<0.01), different lowercase letters are poor
It is different significantly (P<0.05), identical lowercase letter indication difference not significantly (P>0.05)。
As known from Table 2, relative to control group, 3 test group heads daily ingestion amount, average head daily gain are significantly increased it
It is middle experiment 3 groups and control group difference extremely significantly (P<0.01), experiment 1,2 groups and control group significant difference (P<0.05), three examinations
Test between group without significant difference.The price of deed has larger improvement, and feedstuff-meat ratio is substantially reduced, to test 3 groups of effects preferably, with control group
In significant difference (P<0.05).The morbidity head of test group is below than control group, and in actual production, test group pig
The course of disease of test group pig substantially, medicine less investment shorter than control group pig, the economic benefit that test group is obtained also is above
Control group.
Comprehensive conclusion, shows to use nonreactive grower pigs fermented feed of the present invention, energy in large-scale pig farm according to result of the test
Enough significantly improve the production performance and immune performance of pig, promote the health of animal body, reduce making for chemicals or antibiotic
With the overall economic benefit of raising.
Described above is only the preferred embodiment of the present invention, but protection scope of the present invention is not limited thereto, to appointing
For what those of ordinary skill in the art, technique according to the invention scheme and its inventive concept on the basis of the present invention adds
With equivalent substitution or change, it should all be included within the scope of the present invention.
Claims (6)
1. a kind of nonreactive grower pigs fermented feed, it is characterised in that including below according to the raw material of mass percent meter：Microorganism
Leavening 1.0% -2.0%, growth pig feed 42% -71% and water 28% -56%.
2. microbe leaven according to claim 1, it is characterised in that by mixing lactic acid bacteria, S. cervisiae and withered
Careless bacillus, expands after numerous culture respectively through secondary seed, according to mass ratio 3：1：1 mixing is produced.
3. mixing lactic acid bacteria according to claim 2, S. cervisiae and bacillus subtilis, it is characterised in that divide equally
Two steps are prepared：
Step 1：The preparation of first order seed
By lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, lactose lactobacillus, S. cervisiae and withered grass gemma
Bacillus, respectively optimal primary-seed medium and under the conditions of cultivate, obtain corresponding first order seed；
Step 2：The preparation of secondary seed
（1）The preparation of mixing lactic acid bacteria secondary seed, by lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, enterococcus faecalis, breast
Sugared lactobacillus first order seed in mass ratio 2：2：2：1：1 mixing, by inoculum concentration 10%, is inoculated in lactic acid bacteria secondary seed medium,
48-72h of quiescent culture under the conditions of 30-37 DEG C, viable count reaches 1010Cfu/mL, produces mixing lactic acid bacteria secondary seed；
（2）The preparation of S. cervisiae secondary seed, by S. cervisiae first order seed, by inoculum concentration 8%, is inoculated in two grades of kinds
Sub- culture medium, cultivates 9-12h under the conditions of 30-35 DEG C, 160-210r/min of rotating speed, and viable count reaches 108 Cfu/mL, i.e.,
Obtain saccharomyces cerevisiae secondary seed；
（3）The preparation of bacillus subtilis secondary seed, by bacillus subtilis first order seed, by inoculum concentration 8%, is inoculated in two
Level seed culture medium, cultivates 18-24h under the conditions of 35-43 DEG C, 180-240r/min of rotating speed, and viable count reaches 1010 cfu/
ML, produces bacillus subtilis secondary seed.
4. growth pig feed according to claim 1, it is characterised in that include the raw material of following mass percent：Corn
53-70%, dregs of beans 17-27%, palm kernel meal 3-10%, two grades of fish meal 0-3% of import, wheat bran 4-8%, soya-bean oil 0-2%, it is mould proof
Agent 0.1%, premix 4%.
5. the production method of a kind of nonreactive grower pigs fermented feed according to claim 1-4, it is characterised in that by institute
State raw material to be mixed evenly, compacting vacuumizes sealing after pack, and in 26-35 DEG C of 48-72h of anaerobic fermentation, pH value drops to
Less than 4.4, and have strong sour fragrance, you can add and use in the full price basal diet of grower pigs.
6. the application method of nonreactive grower pigs fermented feed according to claim 5, it is characterised in that nonreactive grower pigs are sent out
Ferment feed addition in full price basal diet is 20% -50%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887498A (en) * | 2018-08-07 | 2018-11-27 | 湖南粒丰生物科技有限公司 | For the nonreactive fermented concentrated feed of milking sow and its application |
| CN109793101A (en) * | 2019-03-12 | 2019-05-24 | 江西微源农业科技有限公司 | A kind of biological fermentation feed and production method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410410A (en) * | 2015-12-29 | 2016-03-23 | 雏鹰农牧集团股份有限公司 | Pig complete fermented feed and preparation method thereof |
| CN106889330A (en) * | 2017-03-01 | 2017-06-27 | 海南青牧原实业有限公司 | A kind of production method of nonreactive fermented feed for fattening pigs |
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2017
- 2017-04-06 CN CN201710220838.5A patent/CN106962643A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410410A (en) * | 2015-12-29 | 2016-03-23 | 雏鹰农牧集团股份有限公司 | Pig complete fermented feed and preparation method thereof |
| CN106889330A (en) * | 2017-03-01 | 2017-06-27 | 海南青牧原实业有限公司 | A kind of production method of nonreactive fermented feed for fattening pigs |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887498A (en) * | 2018-08-07 | 2018-11-27 | 湖南粒丰生物科技有限公司 | For the nonreactive fermented concentrated feed of milking sow and its application |
| CN109793101A (en) * | 2019-03-12 | 2019-05-24 | 江西微源农业科技有限公司 | A kind of biological fermentation feed and production method |
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