CN111109465A - Biological feed for pigs and preparation method thereof - Google Patents
Biological feed for pigs and preparation method thereof Download PDFInfo
- Publication number
- CN111109465A CN111109465A CN202010056137.4A CN202010056137A CN111109465A CN 111109465 A CN111109465 A CN 111109465A CN 202010056137 A CN202010056137 A CN 202010056137A CN 111109465 A CN111109465 A CN 111109465A
- Authority
- CN
- China
- Prior art keywords
- parts
- pigs
- fermentation
- feed
- biological feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000282887 Suidae Species 0.000 title claims abstract description 95
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 241000894006 Bacteria Species 0.000 claims abstract description 97
- 238000000855 fermentation Methods 0.000 claims abstract description 94
- 230000004151 fermentation Effects 0.000 claims abstract description 94
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 89
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 46
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 45
- 239000004310 lactic acid Substances 0.000 claims abstract description 44
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 44
- 239000000843 powder Substances 0.000 claims abstract description 41
- 240000008042 Zea mays Species 0.000 claims abstract description 34
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 34
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 34
- 235000005822 corn Nutrition 0.000 claims abstract description 34
- 239000000463 material Substances 0.000 claims abstract description 34
- 235000012054 meals Nutrition 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 27
- 235000009566 rice Nutrition 0.000 claims abstract description 27
- 239000004382 Amylase Substances 0.000 claims abstract description 22
- 102000013142 Amylases Human genes 0.000 claims abstract description 22
- 108010065511 Amylases Proteins 0.000 claims abstract description 22
- 235000019418 amylase Nutrition 0.000 claims abstract description 22
- 238000002156 mixing Methods 0.000 claims abstract description 21
- 102100032487 Beta-mannosidase Human genes 0.000 claims abstract description 20
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 20
- 108010055059 beta-Mannosidase Proteins 0.000 claims abstract description 20
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 20
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 19
- 102000005840 alpha-Galactosidase Human genes 0.000 claims abstract description 19
- 108010030291 alpha-Galactosidase Proteins 0.000 claims abstract description 19
- 235000019779 Rapeseed Meal Nutrition 0.000 claims abstract description 17
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 17
- 239000004456 rapeseed meal Substances 0.000 claims abstract description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 12
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 102000010911 Enzyme Precursors Human genes 0.000 claims abstract description 8
- 108010062466 Enzyme Precursors Proteins 0.000 claims abstract description 8
- 238000009792 diffusion process Methods 0.000 claims abstract description 8
- 239000010903 husk Substances 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract 4
- 239000002994 raw material Substances 0.000 claims description 41
- 102000004190 Enzymes Human genes 0.000 claims description 30
- 108090000790 Enzymes Proteins 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 9
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 5
- 239000000460 chlorine Substances 0.000 claims description 5
- 229910052801 chlorine Inorganic materials 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 2
- 239000011378 shotcrete Substances 0.000 abstract description 11
- 235000019621 digestibility Nutrition 0.000 abstract description 10
- 230000000968 intestinal effect Effects 0.000 abstract description 7
- 229940088598 enzyme Drugs 0.000 description 26
- 241000209094 Oryza Species 0.000 description 23
- 235000015097 nutrients Nutrition 0.000 description 19
- 239000000126 substance Substances 0.000 description 17
- 235000019764 Soybean Meal Nutrition 0.000 description 14
- 239000004455 soybean meal Substances 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- 210000001035 gastrointestinal tract Anatomy 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 235000021050 feed intake Nutrition 0.000 description 10
- 230000036039 immunity Effects 0.000 description 10
- 235000016709 nutrition Nutrition 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 230000029058 respiratory gaseous exchange Effects 0.000 description 10
- 238000003756 stirring Methods 0.000 description 10
- 238000003860 storage Methods 0.000 description 10
- 206010012735 Diarrhoea Diseases 0.000 description 9
- 230000029087 digestion Effects 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 8
- 235000019629 palatability Nutrition 0.000 description 8
- 229920002472 Starch Polymers 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 235000019698 starch Nutrition 0.000 description 7
- 239000008107 starch Substances 0.000 description 7
- 230000002195 synergetic effect Effects 0.000 description 7
- 241000186660 Lactobacillus Species 0.000 description 6
- 239000003925 fat Substances 0.000 description 6
- 235000019197 fats Nutrition 0.000 description 6
- 229940039696 lactobacillus Drugs 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000000433 anti-nutritional effect Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 239000002207 metabolite Substances 0.000 description 5
- 238000004806 packaging method and process Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 229920001221 xylan Polymers 0.000 description 4
- 150000004823 xylans Chemical class 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 102000004882 Lipase Human genes 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 239000004472 Lysine Substances 0.000 description 3
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 102000038379 digestive enzymes Human genes 0.000 description 3
- 108091007734 digestive enzymes Proteins 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 239000001814 pectin Substances 0.000 description 3
- 235000010987 pectin Nutrition 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 150000004804 polysaccharides Chemical class 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
- 229920000057 Mannan Polymers 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229960005069 calcium Drugs 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 235000001465 calcium Nutrition 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 210000004913 chyme Anatomy 0.000 description 2
- 229940079919 digestives enzyme preparation Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000007413 intestinal health Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 235000015277 pork Nutrition 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 235000004347 Perilla Nutrition 0.000 description 1
- 244000124853 Perilla frutescens Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- -1 lactic acid Chemical class 0.000 description 1
- 235000020997 lean meat Nutrition 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 239000002341 toxic gas Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a preparation method of a biological feed for pigs, which comprises the following steps: s1: crushing 220 parts of corn in 130-grade materials, 80-280 parts of bean pulp, 30-90 parts of rapeseed meal, 200 parts of gunite corn husk in 110-grade materials, 80-220 parts of cottonseed meal, 260 parts of bran in 160-grade materials, 200 parts of rice bran meal in 130-grade materials and 40-90 parts of rice hull powder, and mixing to obtain dry powder materials; s2: adding 12-25 parts of lactic acid bacteria, 20-35 parts of active yeast and 13-23 parts of bacillus into 230-300 parts of water for diffusion to obtain a zymogen liquid; s3: adding 15-25 parts of amylase, 10-18 parts of alpha-galactosidase, 5-10 parts of xylanase, 10-15 parts of mannase and 5-10 parts of pectinase into the fermentation broth, and fermenting to obtain a fermentation broth; s4: uniformly spraying the fermentation liquor into the dry powder, and uniformly mixing to obtain a fermentation material; s5: a biological feed obtained by fermenting the fermentation material; the biological feed prepared by the invention can maintain the balance of the intestinal flora of the pigs, improve the digestibility of the feed and increase the growth speed of the pigs.
Description
Technical Field
The invention relates to the technical field of biological feed, and particularly relates to biological feed for pigs and a preparation method thereof.
Background
The pork is sweet in taste, salty and mild, contains rich protein, fat, carbohydrate, calcium, iron, phosphorus and other nutritional ingredients, is a main subsidiary food in daily life, has the effects of tonifying deficiency, building up body, nourishing yin, moistening dryness and enlarging skin, and is deeply loved by people.
At present, pig feed is adopted to feed pigs in batches, and the prior Chinese patent application with the reference of the publication number of CN109123162A discloses pig feed which comprises the following raw materials in parts by weight: 200 parts of wheat germ, 30-60 parts of bean pulp, 20-50 parts of fermented bean pulp, 20-50 parts of corn residue, 10-30 parts of bone meal, 1-3 parts of calcium lactate, 0.01-0.05 part of calcium bicarbonate, 10-15 parts of glucose, 0.001-0.003 part of composite organic mineral, 5-10 parts of purple perilla and 20-40 parts of spinach.
In order to promote the rapid growth of the pigs, the feed often contains a large amount of macromolecular nutrient substances which are not easy to digest by the pigs, so when the ordinary feed is used for feeding the pigs, the digestibility of the feed is low, the intestinal flora of the pigs is easy to unbalance, the immunity is reduced, and the growth speed of the pigs is influenced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a preparation method of a biological feed for pigs, and the prepared biological feed can improve the palatability of the feed, maintain the intestinal flora balance of the pigs, improve the digestibility of the feed and increase the growth speed of the pigs.
The technical purpose of the invention is realized by the following technical scheme:
a preparation method of biological feed for pigs comprises the following steps,
(1) treatment of raw materials:
respectively crushing 220 parts of 130-charge corn, 80-280 parts of bean pulp, 30-90 parts of rapeseed meal, 200 parts of 110-charge guniting corn husk, 80-220 parts of cottonseed meal, 260 parts of bran, 200 parts of 130-charge rice bran meal and 40-90 parts of rice hull powder to the particle size of less than 2.0 mm;
(2) preparation of dry powder: uniformly mixing the raw materials in the step (1) to obtain a dry powder;
(3) preparing a fermentation material:
s1: adding the mixed bacteria into 300 parts of 230-one water for diffusion propagation to obtain a zymogen liquid; wherein the mixed bacteria comprise 12 to 25 parts of lactic acid bacteria, 20 to 35 parts of active yeast and 13 to 23 parts of bacillus by weight;
s2: adding an enzyme preparation into the fermentation broth, and fermenting to obtain a fermentation broth; wherein, the enzyme preparation comprises 15-25 parts of amylase, 10-18 parts of alpha-galactosidase, 5-10 parts of xylanase, 10-15 parts of mannase and 5-10 parts of pectinase by weight;
s3: uniformly spraying the fermentation liquor into the dry powder, and uniformly mixing to obtain a fermentation material;
(4) constant-temperature fermentation: and fermenting the fermented material to obtain the biological feed.
By adopting the technical scheme, the formula is designed by combining the physiological characteristics of the pigs, the comprehensiveness of the feed nutrition, the most excellent strains and the matched production process are fully considered when the fermentation substrate is selected, the formula is designed according to the pig body requirements and beneficial bacteria and metabolites generated after fermentation, and the animal requirements can be well met. Therefore, the biological feed prepared by the invention is not a simple functional feed, and has the functions of rich nutrition, health care, ecological environmental protection and the like.
The corn in the formula of the invention contains rich nutrient substances such as protein, fat, vitamins, trace elements, cellulose and the like, can provide rich nutrient substances for pigs, and is an important raw material for preparing pig feed. The bean pulp is rich in nutritional ingredients such as protein, oil and fat, amino acid and the like. The rapeseed meal contains rich lysine, macroelements and microelements, wherein the content of calcium, selenium, iron, magnesium, manganese and zinc is higher than that of the soybean meal, the content of phosphorus is 2 times of that of the soybean meal, and the rapeseed meal also contains rich sulfur-containing amino acid which is lacked by the soybean meal, so that the rapeseed meal and the soybean meal can play a balance and complementary role when being matched for use, and the nutritional ingredients in the feed are further improved. The cottonseed meal contains a large amount of arginine and less lysine, and the soybean meal is rich in lysine, so that the cottonseed meal and the soybean meal can play a balance and complementary role when being matched. The corn bran is sprayed with the corn steep liquor rich in protein and energy, so that the protein content and the energy of the corn bran are greatly increased, the sprayed corn bran is fragrant in smell and good in taste, the palatability of the feed can be improved, and the feed intake of pigs is improved.
The biological feed adopts the bacteria and enzyme to carry out the synergistic fermentation, so that the feed raw materials and the microorganisms are combined by the fermentation technology and the enzyme engineering technology to produce and prepare the biological feed. Through the synergistic fermentation of bacterial enzymes, macromolecular proteins, carbohydrates, fats and the like in the feed raw materials are degraded into small peptides, amino acids, oligosaccharides, free fatty acids and the like which are easily absorbed by pigs, the digestibility and nutrition of the feed are increased, the anti-nutritional factors in the feed raw materials can be reduced, the feed can be digested and absorbed by the pigs, the balance of the flora in the digestive tract can be ensured, the digestibility of the feed is improved, diarrhea is prevented, and the immunity is improved.
The mixed bacteria comprise lactic acid bacteria, active yeast and bacillus, and the fermentation of the lactic acid bacteria can generate organic acids such as lactic acid, so that the pH of a fermentation system is reduced, the growth of mixed bacteria is inhibited, the proliferation of beneficial bacteria is promoted, and the balance of intestinal flora of pigs is guaranteed. Meanwhile, the lactobacillus fermentation can generate flavor amino acid, so that the biological feed has special acid flavor, stimulates the appetite of pigs, promotes the feed intake and improves the growth speed.
The yeast cells of the active yeast contain various nutrient components such as mannan, glucan, amino acid, protein, vitamins, trace elements, mineral chelate, unknown factors and the like, and can be used as nutrient substances of a host. In addition, the active yeast can decompose sucrose in the nutrient substances into fructose and glucose, provide a carbon source for the lactic acid bacteria and promote the growth and the propagation of the lactic acid bacteria. Since metabolites such as lactate and casein produced by lactic acid bacteria provide energy for the growth of active yeast, the metabolites of lactic acid bacteria and active yeast have complementary mechanisms, and can mutually utilize the respective metabolites to promote the growth.
The bacillus can generate a plurality of enzymes such as protease, amylase, cellulase, lipase and the like in the growth and metabolism process, the enzymes can degrade macromolecular protein, cellulose, carbohydrate, fat and the like in feed raw materials to increase the digestibility and nutrition of the feed, spores with strong heat resistance and stress resistance can be generated and can survive in the gastrointestinal tract of a pig, so that the growth of harmful bacteria in the intestinal tract can be competitively inhibited, the intestinal health of the pig is maintained, diarrhea is prevented, and the immunity is improved. In addition, bacillus can decompose organic substances, organic sulfides, organic nitrogen and the like which generate malodorous gases, and improve the environment of a piggery.
Therefore, the lactic acid bacteria, the active yeast and the bacillus have a synergistic effect on the raw materials, the active yeast can provide enough carbon sources for the lactic acid bacteria and the bacillus to promote the growth of the lactic acid bacteria and the bacillus, and metabolic products of the lactic acid bacteria and the bacillus provide energy sources for the growth of the active yeast, so that the lactic acid bacteria, the active yeast and the bacillus can be complemented and mutually promoted to grow, the feed fermented by the zymocyte liquid in the ratio has better palatability, the feed intake can be increased, and the rapid growth of pigs can be promoted.
The enzyme preparation comprises amylase, alpha-galactosidase, xylanase, mannase and pectinase, 60-80% of energy required by pigs comes from starch in feed raw materials, the amylase is a key enzyme for starch digestion and absorption, and the added amylase can improve the digestion and absorption of the pigs to the starch in the feed, improve the production performance and improve the utilization rate of the feed.
The alpha-galactosidase can decompose the non-starch polysaccharide in the feed into xylo-oligosaccharide with smaller polymerization degree, thereby improving the feed performance and eliminating or reducing the anti-nutritional effect of the non-starch polysaccharide in the intestines and stomach of pigs caused by larger viscosity. In addition, the a-galactosidase can degrade flatulence factors in the soybean meal, and the phenomenon of pig diarrhea caused by the soybean meal is greatly reduced.
The feed raw materials such as corn, bran, rice bran meal and the like contain a certain amount of xylan, and the xylan is one of main components forming cell walls and plays a physical barrier role together with other cell wall constituents to prevent the release of nutrient substances in cells. The xylanase breaks the firm structure of the cell wall by degrading xylan, releases nutrients such as protein, starch, fat and the like wrapped in the cell wall, can eliminate the anti-nutritional effect of the xylan in the feed raw materials, and improves the digestibility of the nutrients.
The mannase can decompose mannan in feed raw materials such as soybean meal, cottonseed meal and the like, and improve the capability of the intestinal tract of animals to absorb water and glucose, thereby reducing the morbidity of nutritional diarrhea, reducing the viscosity of the intestinal tract, and promoting the digestion and absorption of energy, protein and cellulose. In addition, the mannase can promote the secretion of insulin-like growth factors in animal bodies, promote the synthesis of proteins, improve the lean meat percentage and promote the growth of pigs.
The bean pulp, the rapeseed meal and the cottonseed meal contain a certain amount of pectin, and the pectin is dissolved in water to be in a colloidal state, so that the viscosity of chyme is increased, the release of nutrient substances is hindered, the activity of digestive enzyme is influenced, the digestion and absorption of the nutrient substances are reduced, and the pectin is an important anti-nutritional factor in the feed. The addition of pectinase can destroy the cell wall of plant cell, reduce chyme degree, improve the activity of digestive enzyme, promote the absorption and digestion of nutrient substances, and improve the utilization rate of feed. In addition, the addition of pectinase can obviously improve the activity of amylase, lipase and trypsin of duodenum and pancreas and the activity of lipase of jejunum, thereby improving the digestion and absorption of nutrient substances in the feed and promoting the rapid growth of pig organisms.
Amylase, alpha-galactosidase, xylanase, mannase and pectinase are mixed to form a complex enzyme preparation, the problem of insufficient endogenous enzymes in a pig body is fully solved, the complex enzyme preparation is mixed with lactobacillus, active yeast and bacillus to form fermentation liquor, and the bacterial enzymes are fermented in a synergistic manner, so that the utilization rate of the feed can be effectively improved, the digestive system of the pig on nutrient components in the feed is promoted, and the feed intake is improved. In addition, the health food can maintain the balance of intestinal flora, reduce diarrhea incidence and enhance immunity.
The fermentation liquor with reasonable proportion added in the formula of the invention has synergistic effect with the feed raw materials, so that the conversion rate of the feed is improved, the growth speed of pigs is promoted, and NH in pig manure is reduced3、H2The content of S can weaken the foul smell of the excrement, improve the environment of the pigsty, and also can improve the immunity of the pigs so as to ensure that the pigs grow healthily and rapidly.
In conclusion, the specific raw materials are selected in the formula, the proportion of the raw materials is limited, the specific mixed bacteria and the enzyme preparation are selected according to the specific raw materials, and the proportion of the mixed bacteria and the enzyme preparation is limited to prepare the specific fermentation broth, so that the specific fermentation broth can better ferment the specific raw materials, the nutrient components of the feed are richer, the utilization rate of the feed is improved, the balance of intestinal flora of pigs can be maintained, the incidence rate of diarrhea is reduced, and the immunity is enhanced.
In a preferred example, the invention can be further configured that the raw materials in the step (1) comprise, by weight, 150-200 parts of corn-;
the mixed bacteria in the step S1 comprise 17-23 parts of lactic acid bacteria, 23-30 parts of active yeast and 15-21 parts of bacillus;
the enzyme preparation in the step S2 comprises 18-22 parts of amylase, 12-16 parts of alpha-galactosidase, 7-9 parts of xylanase, 12-14 parts of mannase and 6-8 parts of pectinase.
By adopting the technical scheme, experiments show that the proportion of the raw materials, the mixed bacteria and the enzyme preparation is reduced, the utilization rate of the obtained feed is better, the feed can be digested and absorbed by pigs, the balance of the flora in the digestive tract can be ensured, the digestibility of the feed is improved, diarrhea is prevented, and the immunity is improved. The reason for this analysis may be that the synergy between the feedstock and the fermentation broth is stronger in this range, enabling better fermentation of the feedstock.
The present invention in a preferred example may be further configured to: in the step S1, before the mixed bacteria are added, water is heated to 35-37 ℃, then 15-20 parts of brown sugar is added to dissolve the water, and then the mixed bacteria are added.
By adopting the technical scheme, the temperature of the fermentation material can be increased by heating the fermentation water so as to start the fermentation as soon as possible, thereby inhibiting the proliferation of harmful bacteria contained in the feed raw materials and improving the production efficiency. The brown sugar is rich in sugar, mineral substances and glycolic acid, and also contains vitamins and trace elements, and the brown sugar can provide energy substances for the mixed bacteria, increase the activity of the flora and improve the fermentation efficiency; on the other hand, after fermentation, a certain sweet-sour ratio can be formed, the palatability is better, and the feed intake of the pigs is improved, so that the rapid growth of the pigs is promoted.
The present invention in a preferred example may be further configured to: in the step S1, the weight ratio of the lactic acid bacteria, the active yeast and the bacillus is 1: (1.2-1.6): (0.9-1.1).
By adopting the technical scheme, tests show that the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1: (1.2-1.6): (0.9-1.1), the pig has better feed intake, lower morbidity and faster growth rate. The reason for analyzing the ratio may be that in the ratio range, the complementary mechanism among the lactic acid bacteria, the active yeast and the bacillus is stronger, and the lactic acid bacteria, the active yeast and the bacillus can better mutually promote and quickly grow, so that the palatability of the fermented liquid fermented feed in the ratio is better, the feed intake can be improved, and the quick growth of pigs can be promoted.
The present invention in a preferred example may be further configured to: in the step S2, the fermentation temperature after the enzyme preparation is added is 35-37 ℃, and the fermentation time is 16-24 h.
By adopting the technical scheme, the temperature suitable for the growth of the mixed bacteria and the enzyme preparation is 35-37 ℃, so that the mixed bacteria and the enzyme preparation can keep higher activity after the enzyme preparation is added and the fermentation is carried out at the temperature of 35-37 ℃, the rapid proliferation of the mixed bacteria is promoted, and the fermentation efficiency is improved.
The present invention in a preferred example may be further configured to: in the step (4), the fermentation temperature is controlled to be 25-28 ℃, and the fermentation time is 5-7 days.
The present invention in a preferred example may be further configured to: in step S1, the water is heated before the mixed bacteria are added to remove chlorine in the water.
By adopting the technical scheme, chlorine contained in the feed is reduced, gastrointestinal tract reactions such as inappetence and diarrhea after eating the feed are avoided, the disease rate of pigs is reduced, and the healthy and rapid growth of the pigs is ensured. In addition, the chlorine is removed by adopting a heating method, and simultaneously, the fermentation water is heated to the fermentation temperature, so that two purposes are achieved, and the energy is saved.
The invention also aims to provide a biological feed for pigs prepared by the method of any one of claims 1-7.
In summary, the invention includes at least one of the following beneficial technical effects:
1. the biological feed provided by the invention has high feed conversion rate, and can improve the digestibility of nutrient components; the microbial metabolite produced by fermentation can improve the palatability of the feed, increase the feed intake of pigs, promote the growth speed of the pigs and improve the product quality of pork.
2. In the formula of the feed additive, the fermentation liquor and the feed raw materials have a synergistic effect, and the fermentation liquor can decompose macromolecules in the feed raw materials, so that nutritional ingredients in the feed raw materials are more easily absorbed by pigs; the feed raw materials can also provide nutrients for mixed bacteria and enzyme preparations in the fermentation solution, so that the activity of the mixed bacteria and enzyme preparations is improved, and after the biological feed is eaten by pigs, the intestinal health of the pigs can be improved, the immunity of the pigs is improved, the gastrointestinal development of piglets is stimulated, the acid environment of the digestive tract can be maintained, the secretion of endogenous digestive enzymes and the enzyme activity are stimulated, the acid-series capacity of daily ration is reduced, and the acidification of the feed is promoted.
3. According to the formula, a large amount of beneficial mixed bacteria are adopted for fermentation, so that the digestion and absorption functions of pigs are promoted, nutrient substances in the feed are fully absorbed and utilized, the utilization rate of the feed is improved, the flora balance of pig intestinal tracts is kept, waste generated after intestinal tract digestion is reduced, and the emission of ammonia gas is reduced. In addition, a large amount of lactic acid bacteria inhibit harmful bacteria in intestinal tracts, reduce the generation of endotoxin, promote gastrointestinal peristalsis, and generate organic acid which can increase the humidity of excrement, quickly relieve constipation and reduce the constipation phenomenon.
4. In the formula of the invention, the mixed bacteria and the enzyme preparation are fermented coordinately, so that the anti-nutritional factors in the feed can be effectively removed, and the digestibility of the nutritional ingredients in the feed is improved.
5. According to the invention, brown sugar is added into the formula, so that on one hand, energy substances can be provided for mixed bacteria, the activity of flora is increased, and the fermentation efficiency is improved; on the other hand, after fermentation, the feed has a certain sweet-sour ratio, has better palatability, and is beneficial to improving the feed intake of pigs, thereby promoting the rapid growth of the pigs.
Detailed Description
The present invention will be described in further detail with reference to examples.
In the following examples and comparative examples:
the spraying corn bran is purchased from Xian's national vitamin starch, and has limited responsibility;
lactic acid bacteria, active yeast, bacillus, amylase, alpha-galactosidase, xylanase, mannanase and pectinase are all purchased from national engineering research center for biological feed development.
Example 1
The biological feed for pigs comprises the following components in parts by weight: 130 parts of corn, 80 parts of soybean meal, 30 parts of rapeseed meal, 110 parts of gunite corn bran, 80 parts of cottonseed meal, 160 parts of bran, 130 parts of rice bran meal, 40 parts of rice hull powder, 12 parts of lactic acid bacteria, 20 parts of active yeast, 13 parts of bacillus, 15 parts of amylase, 10 parts of alpha-galactosidase, 5 parts of xylanase, 10 parts of mannase, 5 parts of pectinase and 230 parts of water;
the preparation method of the biological feed for the pigs comprises the following steps:
(1) treatment of raw materials:
respectively crushing corn, bean pulp, rapeseed meal, gunite corn bran, cottonseed meal, bran, rice bran meal and rice hull powder, and screening by using a crushing screen with a screen hole of 2.0 mm;
(2) preparation of dry powder: adding the dry powder raw materials in the step (1) into a mixer, stirring, and uniformly mixing to obtain dry powder;
(3) preparing a fermentation material:
s1: mixing lactic acid bacteria, active yeast and bacillus to obtain mixed bacteria, heating water to remove chlorine, keeping the temperature at 35 ℃, adding the mixed bacteria into the water, and performing diffusion propagation to obtain zymogen liquid;
s2: adding amylase, alpha-galactosidase, xylanase, mannase and pectinase into the fermentation broth, stirring, and fermenting for 16h to obtain fermentation broth;
s3: uniformly spraying the fermentation liquor obtained in the step S2 into the dry powder obtained in the step (2), and uniformly mixing to obtain a fermentation material;
s4: respectively filling the fermented materials into breathing bags and packaging;
(4) constant-temperature fermentation: and (3) putting the breathing bag filled with the fermentation material into a constant-temperature storage, controlling the temperature of the constant-temperature storage at 25 ℃, and fermenting for 5 days to obtain the biological feed.
Example 2
The biological feed for pigs comprises the following components in parts by weight: 150 parts of corn, 100 parts of soybean meal, 40 parts of rapeseed meal, 130 parts of gunite corn bran, 100 parts of cottonseed meal, 180 parts of bran, 150 parts of rice bran meal, 50 parts of rice hull powder, 17 parts of lactic acid bacteria, 23 parts of active yeast, 15 parts of bacillus, 18 parts of amylase, 12 parts of alpha-galactosidase, 7 parts of xylanase, 12 parts of mannase, 6 parts of pectinase and 250 parts of water;
the preparation method of the biological feed for the pigs comprises the following steps:
(1) treatment of raw materials:
respectively crushing corn, bean pulp, rapeseed meal, gunite corn bran, cottonseed meal, bran, rice bran meal and rice hull powder, and screening by using a crushing screen with a screen hole of 2.0 mm;
(2) preparation of dry powder: adding the dry powder raw materials in the step (1) into a mixer, stirring, and uniformly mixing to obtain dry powder;
(3) preparing a fermentation material:
s1: mixing lactobacillus, active yeast and bacillus to obtain mixed bacteria, keeping the temperature at 35.5 ℃, and adding the mixed bacteria into water for diffusion propagation to obtain zymogen liquid;
s2: adding amylase, alpha-galactosidase, xylanase, mannase and pectinase into the fermentation broth, stirring, and fermenting for 18h to obtain fermentation broth;
s3: uniformly spraying the fermentation liquor obtained in the step S2 into the dry powder obtained in the step (2), and uniformly mixing to obtain a fermentation material;
s4: respectively filling the fermented materials into breathing bags and packaging;
(4) constant-temperature fermentation: and (3) putting the breathing bag filled with the fermentation material into a constant temperature storage, controlling the temperature of the constant temperature storage at 26 ℃, and fermenting for 6 days to obtain the biological feed.
Example 3
The biological feed for pigs comprises the following components in parts by weight: 175 parts of corn, 180 parts of soybean meal, 60 parts of rapeseed meal, 155 parts of gunite corn bran, 150 parts of cottonseed meal, 210 parts of bran, 165 parts of rice bran meal, 65 parts of rice hull powder, 18.5 parts of lactic acid bacteria, 27.5 parts of active yeast, 18 parts of bacillus, 21 parts of amylase, 14 parts of alpha-galactosidase, 7.5 parts of xylanase, 12.5 parts of mannase, 7.5 parts of pectinase and 265 parts of water;
the preparation method of the biological feed for the pigs comprises the following steps:
(1) treatment of raw materials:
respectively crushing corn, bean pulp, rapeseed meal, gunite corn bran, cottonseed meal, bran, rice bran meal and rice hull powder, and screening by using a crushing screen with a screen hole of 2.0 mm;
(2) preparation of dry powder: adding the dry powder raw materials in the step (1) into a mixer, stirring, and uniformly mixing to obtain dry powder;
(3) preparing a fermentation material:
s1: mixing lactobacillus, active yeast and bacillus to obtain mixed bacteria, keeping the temperature at 35.5 ℃, and adding the mixed bacteria into water for diffusion propagation to obtain zymogen liquid;
s2: adding amylase, alpha-galactosidase, xylanase, mannase and pectinase into the fermentation broth, stirring, and fermenting for 20h to obtain fermentation broth;
s3: uniformly spraying the fermentation liquor obtained in the step S2 into the dry powder obtained in the step (2), and uniformly mixing to obtain a fermentation material;
s4: respectively filling the fermented materials into breathing bags and packaging;
(4) constant-temperature fermentation: and (3) putting the breathing bag filled with the fermentation material into a constant temperature storage, controlling the temperature of the constant temperature storage at 26.5 ℃, and fermenting for 6 days to obtain the biological feed.
Example 4
The biological feed for pigs comprises the following components in parts by weight: 200 parts of corn, 260 parts of soybean meal, 80 parts of rapeseed meal, 185 parts of gunite corn bran, 200 parts of cottonseed meal, 240 parts of bran, 180 parts of rice bran meal, 80 parts of rice hull powder, 23 parts of lactic acid bacteria, 30 parts of active yeast, 20 parts of bacillus, 22 parts of amylase, 16 parts of alpha-galactosidase, 9 parts of xylanase, 14 parts of mannase, 8 parts of pectinase and 290 parts of water;
the preparation method of the biological feed for the pigs comprises the following steps:
(1) treatment of raw materials:
respectively crushing corn, bean pulp, rapeseed meal, gunite corn bran, cottonseed meal, bran, rice bran meal and rice hull powder, and screening by using a crushing screen with a screen hole of 2.0 mm;
(2) preparation of dry powder: adding the dry powder raw materials in the step (1) into a mixer, stirring, and uniformly mixing to obtain dry powder;
(3) preparing a fermentation material:
s1: mixing lactobacillus, active yeast and bacillus to obtain mixed bacteria, keeping the temperature at 36.5 ℃, and adding the mixed bacteria into water for diffusion propagation to obtain zymogen liquid;
s2: adding amylase, alpha-galactosidase, xylanase, mannase and pectinase into the fermentation broth, stirring, and fermenting for 22h to obtain fermentation broth;
s3: uniformly spraying the fermentation liquor obtained in the step S2 into the dry powder obtained in the step (2), and uniformly mixing to obtain a fermentation material;
s4: respectively filling the fermented materials into breathing bags and packaging;
(4) constant-temperature fermentation: and (3) putting the breathing bag filled with the fermentation material into a constant temperature storage, controlling the temperature of the constant temperature storage at 27 ℃, and fermenting for 7 days to obtain the biological feed.
Example 5
The biological feed for pigs comprises the following components in parts by weight: 220 parts of corn, 280 parts of soybean meal, 90 parts of rapeseed meal, 200 parts of gunite corn bran, 220 parts of cottonseed meal, 260 parts of bran, 200 parts of rice bran meal, 90 parts of rice hull powder, 25 parts of lactic acid bacteria, 35 parts of active yeast, 23 parts of bacillus, 25 parts of amylase, 28 parts of alpha-galactosidase, 10 parts of xylanase, 15 parts of mannase, 10 parts of pectinase and 300 parts of water;
the preparation method of the biological feed for the pigs comprises the following steps:
(1) treatment of raw materials:
respectively crushing corn, bean pulp, rapeseed meal, gunite corn bran, cottonseed meal, bran, rice bran meal and rice hull powder, and screening by using a crushing screen with a screen hole of 2.0 mm;
(2) preparation of dry powder: adding the dry powder raw materials in the step (1) into a mixer, stirring, and uniformly mixing to obtain dry powder;
(3) preparing a fermentation material:
s1: mixing lactobacillus, active yeast and bacillus to obtain mixed bacteria, keeping the temperature at 37 ℃, and adding the mixed bacteria into water for diffusion propagation to obtain zymogen liquid;
s2: adding amylase, alpha-galactosidase, xylanase, mannase and pectinase into the fermentation broth, stirring, and fermenting for 24 hr to obtain fermentation broth;
s3: uniformly spraying the fermentation liquor obtained in the step S2 into the dry powder obtained in the step (2), and uniformly mixing to obtain a fermentation material;
s4: respectively filling the fermented materials into breathing bags and packaging;
(4) constant-temperature fermentation: and (3) putting the breathing bag filled with the fermentation material into a constant-temperature storage, controlling the temperature of the constant-temperature storage at 28 ℃, and fermenting for 7 days to obtain the biological feed.
Example 6
A biological feed for pigs, which is different from example 3 in that 15 parts of brown sugar is added to water and sufficiently dissolved before adding mixed bacteria in step S1.
Example 7
A biological feed for pigs, which is different from example 3 in that 17.5 parts of brown sugar is added to water and sufficiently dissolved before adding mixed bacteria in step S1.
Example 8
A biological feed for pigs, which is different from example 3 in that 20 parts of brown sugar is added to water and sufficiently dissolved before adding mixed bacteria in step S1.
Example 9
The biological feed for pigs is different from the biological feed in example 3 in that the components of the mixed bacteria comprise 20 parts of lactic acid bacteria, 24 parts of active yeast and 18 parts of bacillus according to parts by weight, namely the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1: 1.2: 0.9.
example 10
The biological feed for the pigs is different from the biological feed in example 3 in that the components of the mixed bacteria comprise 18 parts of lactic acid bacteria, 25.2 parts of active yeast and 18 parts of bacillus according to parts by weight, namely the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1:1.4: 1.0.
Example 11
The biological feed for pigs is different from the biological feed in example 3 in that the components of the mixed bacteria comprise 17 parts by weight of lactic acid bacteria, 27.2 parts by weight of active yeast and 18.7 parts by weight of bacillus, namely the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1: 1.6: 1.1.
comparative example 1
The biological feed for pigs is different from the biological feed in example 10 in that the components of the mixed bacteria comprise 23 parts of lactic acid bacteria, 25.3 parts of active yeast and 18.4 parts of bacillus according to parts by weight, namely the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1: 1.1: 0.8.
comparative example 2
The biological feed for pigs is different from the biological feed in example 10 in that the components of the mixed bacteria comprise 17 parts by weight of lactic acid bacteria, 28.9 parts by weight of active yeast and 18 parts by weight of bacillus, namely the weight ratio of the lactic acid bacteria to the active yeast to the bacillus is 1: 1.7: 1.2.
comparative example 3
The feed for ordinary pigs is sold on the market.
Feeding experiment
Selecting 1400 healthy and disease-free pigs of the same variety with weight average of 50 + -1 Kg, normal growth and development, and average division into 14 groups, and placing in the same ringIn 14 pigsties, the pigs were fed with the feed of examples 1-11 and comparative examples 1-3, respectively, for a period of 30 days. The feed was timed to 2 times at 8:30 am and 5:30 pm daily. 3 times daily drinking water, 9:30 in the morning and 12 in the noon: 30 in the afternoon at 6:30 to ensure that each pig drinks water fully. For NH in the pigsty after 30 days3The contents (with PGM-35 toxic gas detector), the number of pigs ill within 30 days, and the average daily gain per pig per group were analyzed and the results are shown in Table 1.
TABLE 1 feed test results
| Ammonia concentration in house (PPM) | Number of patients | Daily gain (g) | |
| Example 1 | 7.9 | 6 | 747 |
| Example 2 | 7.81 | 5 | 756 |
| Example 3 | 7.59 | 3 | 778 |
| Example 4 | 7.74 | 4 | 762 |
| Example 5 | 7.85 | 5 | 752 |
| Example 6 | 7.21 | 2 | 818 |
| Example 7 | 7.01 | 1 | 842 |
| Example 8 | 7.15 | 2 | 825 |
| Example 9 | 6.91 | 1 | 854 |
| Example 10 | 6.61 | 0 | 893 |
| Example 11 | 6.87 | 1 | 859 |
| Comparative example 1 | 7.94 | 7 | 743 |
| Comparative example 2 | 7.98 | 8 | 740 |
| Comparative example 3 | 20.81 | 10 | 651 |
As can be seen from Table 1, when compared with comparative example 3, the biological feeds prepared in examples 1 to 11 were used to feed pigs, which can increase daily gain of pigs, reduce the number of diseases of pigs, and effectively reduce NH in pigsties3The content shows that the biological feed is scientific and reasonable in proportioning, can improve the conversion rate of the feed, improve the immunity of pigs, promote the growth of the pigs, reduce the odor of excrement and improve the environment of a piggery.
In combination with examples 1 to 5, it can be seen that the biological feeds prepared in examples 2 to 4, when used for feeding pigs, can increase the daily gain of pigs, reduce the number of diseases of pigs, and effectively reduce NH in pigsties, compared to examples 1 and 53And (4) content. The proportion of the raw materials, the mixed bacteria and the enzyme preparation is reduced, so that the synergistic effect of the raw materials, the mixed bacteria and the enzyme preparation in the proportion range is stronger, the conversion rate of the fermented biological feed is better, the digestion and absorption of pigs can be better, and the rapid growth of the pigs can be promoted.
As can be seen by combining examples 6-8 with example 3, the addition of brown sugar can increase the daily gain of pigs and reduce the occurrence of diseases in pigsCounting and effectively reducing NH of the pigsty3And (4) content. The brown sugar is added to provide energy substances for the mixed bacteria, increase the activity of the flora and improve the fermentation efficiency, so that the nutrient substances in the prepared biological feed can be thoroughly digested, the excrement is soft and odorless, and the air content of a pigsty is improved; on the other hand, after fermentation, a certain sweet-sour ratio is formed, the palatability is better, and the feed intake of the pigs is improved, so that the rapid growth of the pigs is promoted.
In the combination of examples 9-11 and comparative examples 1-2, the weight ratio of lactic acid bacteria, active yeast and bacillus in the composition of the raw materials of the present invention and the composition of the selected enzyme is 1: (1.2-1.6): (0.9-1.1), the daily gain of the pigs can be improved, the number of diseases of the pigs is reduced, and the content of NH3 in the pigsty can be effectively reduced. The reason is that the active yeast provides sufficient carbon source for the lactic acid bacteria and the bacillus, the metabolic products of the lactic acid bacteria and the bacillus provide energy for the growth of the active yeast, and the lactic acid bacteria, the active yeast and the bacillus mutually promote the growth by a complementary mechanism, so that the lactic acid bacteria, the active yeast and the bacillus have higher activity, and the feed fermentation is more thorough. The fermented metabolite prebiotics active polysaccharide produced by fermentation can regulate intestinal microflora, improve intestinal environment, reduce diarrhea of pigs, improve disease resistance, defense and immunity, and reduce the disease rate of pigs.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (8)
1. A preparation method of biological feed for pigs is characterized by comprising the following steps: comprises the following steps of (a) carrying out,
(1) treatment of raw materials:
respectively crushing 220 parts of 130-charge corn, 80-280 parts of bean pulp, 30-90 parts of rapeseed meal, 200 parts of 110-charge guniting corn husk, 80-220 parts of cottonseed meal, 260 parts of bran, 200 parts of 130-charge rice bran meal and 40-90 parts of rice hull powder to the particle size of less than 2.0 mm;
(2) preparation of dry powder: uniformly mixing the raw materials in the step (1) to obtain a dry powder;
(3) preparing a fermentation material:
s1: adding the mixed bacteria into 300 parts of 230-one water for diffusion propagation to obtain a zymogen liquid;
wherein the mixed bacteria comprise 12 to 25 parts of lactic acid bacteria, 20 to 35 parts of active yeast and 13 to 23 parts of bacillus by weight;
s2: adding an enzyme preparation into the fermentation broth, and fermenting to obtain a fermentation broth;
wherein, the enzyme preparation comprises 15-25 parts of amylase, 10-18 parts of alpha-galactosidase, 5-10 parts of xylanase, 10-15 parts of mannase and 5-10 parts of pectinase by weight;
s3: uniformly spraying the fermentation liquor into the dry powder, and uniformly mixing to obtain a fermentation material;
(4) constant-temperature fermentation: and fermenting the fermented material to obtain the biological feed.
2. The method for preparing a biological feed for pigs according to claim 1, which is characterized in that: the raw materials in the step (1) comprise, by weight, 150-200 parts of corn-;
the mixed bacteria in the step S1 comprise 17-23 parts of lactic acid bacteria, 23-30 parts of active yeast and 15-21 parts of bacillus;
the enzyme preparation in the step S2 comprises 18-22 parts of amylase, 12-16 parts of alpha-galactosidase, 7-9 parts of xylanase, 12-14 parts of mannase and 6-8 parts of pectinase.
3. The method for preparing a biological feed for pigs according to claim 2, which is characterized in that: in the step S1, before the mixed bacteria are added, water is heated to 35-37 ℃, then 15-20 parts of brown sugar is added to dissolve the water, and then the mixed bacteria are added.
4. The method for preparing a biological feed for pigs according to claim 2, which is characterized in that: in the step S1, the weight ratio of the lactic acid bacteria, the active yeast and the bacillus is 1: (1.2-1.6): (0.9-1.1).
5. The method for preparing a biological feed for pigs according to claim 2, which is characterized in that: in the step S2, the fermentation temperature after the enzyme preparation is added is 35-37 ℃, and the fermentation time is 16-24 h.
6. The method for preparing a biological feed for pigs according to claim 2, which is characterized in that: in the step (4), the fermentation temperature is controlled to be 25-28 ℃, and the fermentation time is 5-7 days.
7. The method for preparing a biological feed for pigs according to claim 2, which is characterized in that: in step S1, the water is heated before the mixed bacteria are added to remove chlorine in the water.
8. A biological feed for pigs prepared by the method of any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010056137.4A CN111109465A (en) | 2020-01-18 | 2020-01-18 | Biological feed for pigs and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010056137.4A CN111109465A (en) | 2020-01-18 | 2020-01-18 | Biological feed for pigs and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111109465A true CN111109465A (en) | 2020-05-08 |
Family
ID=70491031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010056137.4A Pending CN111109465A (en) | 2020-01-18 | 2020-01-18 | Biological feed for pigs and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111109465A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642641A (en) * | 2020-06-24 | 2020-09-11 | 天津九州大地饲料有限公司 | Pig feed and preparation method thereof |
| CN112369515A (en) * | 2020-11-13 | 2021-02-19 | 张家口九州大地饲料有限公司 | Supplementary feed for breeding cows and preparation method thereof |
| CN112753871A (en) * | 2020-12-31 | 2021-05-07 | 黑龙江省农业科学院畜牧兽医分院 | Pig feed for improving immunity and preparation method thereof |
| WO2022069084A1 (en) * | 2020-10-02 | 2022-04-07 | Hamlet Protein A/S | Feed or food ingredient derived from fibre-rich biomass of soy hulls |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511652A (en) * | 2011-12-02 | 2012-06-27 | 赤峰市大中高科技饲料有限公司 | Production method for microbial balanced protein feed |
| CN106107064A (en) * | 2016-06-27 | 2016-11-16 | 浙江绿谷牧业有限公司 | The production technology of one boar growth promotion cold granulation fermented feed |
| CN109662208A (en) * | 2019-02-26 | 2019-04-23 | 天津九州大地饲料有限公司 | A kind of preparation process of piglet fermentative feedstuff of microbe |
| CN109662206A (en) * | 2019-02-26 | 2019-04-23 | 天津九州大地饲料有限公司 | A kind of preparation method of biofermentation agent of feed for nursing sow |
| CN109924360A (en) * | 2017-12-19 | 2019-06-25 | 辽宁波尔莱特农牧实业有限公司 | One boar fermentation compound feed and preparation method thereof |
| CN110012964A (en) * | 2019-05-11 | 2019-07-16 | 辽宁九州生物科技有限公司 | A kind of semi-fermented concentrate feed of piglet and preparation method thereof |
| CN111642618A (en) * | 2020-06-24 | 2020-09-11 | 天津九州大地饲料有限公司 | Ruminant fermented feed and preparation method thereof |
-
2020
- 2020-01-18 CN CN202010056137.4A patent/CN111109465A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511652A (en) * | 2011-12-02 | 2012-06-27 | 赤峰市大中高科技饲料有限公司 | Production method for microbial balanced protein feed |
| CN106107064A (en) * | 2016-06-27 | 2016-11-16 | 浙江绿谷牧业有限公司 | The production technology of one boar growth promotion cold granulation fermented feed |
| CN109924360A (en) * | 2017-12-19 | 2019-06-25 | 辽宁波尔莱特农牧实业有限公司 | One boar fermentation compound feed and preparation method thereof |
| CN109662208A (en) * | 2019-02-26 | 2019-04-23 | 天津九州大地饲料有限公司 | A kind of preparation process of piglet fermentative feedstuff of microbe |
| CN109662206A (en) * | 2019-02-26 | 2019-04-23 | 天津九州大地饲料有限公司 | A kind of preparation method of biofermentation agent of feed for nursing sow |
| CN110012964A (en) * | 2019-05-11 | 2019-07-16 | 辽宁九州生物科技有限公司 | A kind of semi-fermented concentrate feed of piglet and preparation method thereof |
| CN111642618A (en) * | 2020-06-24 | 2020-09-11 | 天津九州大地饲料有限公司 | Ruminant fermented feed and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642641A (en) * | 2020-06-24 | 2020-09-11 | 天津九州大地饲料有限公司 | Pig feed and preparation method thereof |
| WO2022069084A1 (en) * | 2020-10-02 | 2022-04-07 | Hamlet Protein A/S | Feed or food ingredient derived from fibre-rich biomass of soy hulls |
| CN116419678A (en) * | 2020-10-02 | 2023-07-11 | 哈姆雷特蛋白质有限公司 | Feed or food ingredient derived from fiber-rich biomass of soybean hulls |
| CN112369515A (en) * | 2020-11-13 | 2021-02-19 | 张家口九州大地饲料有限公司 | Supplementary feed for breeding cows and preparation method thereof |
| CN112753871A (en) * | 2020-12-31 | 2021-05-07 | 黑龙江省农业科学院畜牧兽医分院 | Pig feed for improving immunity and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101485402B (en) | Composite biological feed additive agent for fattening early weaning mutton sheep | |
| CN102972638B (en) | Animal feed additive and preparation method thereof | |
| CN102356822B (en) | Fermented traditional Chinese medicine feed additive for pigs and preparation method thereof | |
| CN102228129B (en) | Fermented feed | |
| CN1224330C (en) | Feed additive containing microbe and biological enzyme | |
| CN110122658A (en) | A kind of fermentative feedstuff of microbe and its preparation method and application | |
| CN111109465A (en) | Biological feed for pigs and preparation method thereof | |
| CN106834163A (en) | Feed fermentation agent, fermented feed and preparation method thereof | |
| CN111642618A (en) | Ruminant fermented feed and preparation method thereof | |
| CN111034868A (en) | Compound bacterium fermented feed and preparation method and application thereof | |
| CN106509432A (en) | Piglet intestinal health improving and weaning stress reducing creep feed and preparation method thereof | |
| CN104814276A (en) | Biological deodorant for animal feeding | |
| CN105053566B (en) | A kind of bicycle beam wood seed Pepsin feed addictive and preparation method thereof | |
| CN104886390A (en) | Biologically fermented pig feed and a preparation method | |
| CN104012797A (en) | Early-stage compound feed for broiler chickens and preparation method thereof | |
| CN105166422A (en) | Biological fermentation feed for accelerating pig growth and preparation method thereof | |
| CN108142728A (en) | Fish fermented feed and its preparation method and application | |
| CN108684936A (en) | A kind of glycyrrhiza extract fermented health-care feed and its production technology | |
| CN108125023A (en) | One boar enzyme-containing formulation feed | |
| CN110037173A (en) | A kind of pomace and dreg fodder and preparation method thereof | |
| KR100369431B1 (en) | A process of alcohol fermentation feed for green tea ang yellow earth and lactic ferments | |
| CN101219154A (en) | Microorganism additive agent for improving phylactic power of pets and accelerating growth | |
| CN104886391A (en) | Biological fermentation feed for reducing odor of pig manure and preparation method of biological fermentation feed | |
| CN102640889B (en) | Detoxifying agent for cottonseed meal and rapeseed meal, preparation method thereof and preparation method of microorganism feed thereby | |
| CN112471332A (en) | Antibiotic-free suckling pig nursing material containing complex enzyme and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200508 |