CN104381607A - Phycomycete complex fermented feed additive and preparation method thereof - Google Patents
Phycomycete complex fermented feed additive and preparation method thereof Download PDFInfo
- Publication number
- CN104381607A CN104381607A CN201410660199.0A CN201410660199A CN104381607A CN 104381607 A CN104381607 A CN 104381607A CN 201410660199 A CN201410660199 A CN 201410660199A CN 104381607 A CN104381607 A CN 104381607A
- Authority
- CN
- China
- Prior art keywords
- phycomycete
- feed additive
- obtains
- fermented
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a phycomycete complex fermented feed additive and a preparation method thereof. The additive is prepared from the following components in parts by weight: 10-40 parts of alga slurry, 5-8 parts of honey, 5-8 parts of residue of soya, 25-35 parts of yeast paste, 15-30 parts of bean pulp, 10-30 parts of complex microbial suspension, 0.01-0.12 part of cellulase and 95-182 parts of water. The preparation method comprises the step of preparing the components by a semi-solid three-stage mixing and fermenting technology. The feed additive product produced by a scientific complex formulation and microbial mixing and fermenting technology is rich in high-quality single-cell protein, active probiotics and multiple hydrolase activities, and can be used for promoting growth of livestock and poultry, effectively improving intestine health of animals, effectively improving animal immunity, reducing usage of antibiotics and improving the conversion rate of feed.
Description
Technical field
The present invention relates to biofermentation and feed additive production field, specifically a kind of phycomycete composite fermented feed additive and preparation method thereof, namely utilize other microbial bacterial agent of unicellular alga compound and fermentation auxiliary material, carry out semi-solid ferment and produce additive for farm animal feed.
Background technology
In recent years, many countries have put into effect corresponding restriction to antibiotic use or have forbidden policy in the world, this makes the task of top priority of setting up in a wide range feed resource, development green ecological livestock and poultry breeding industry becomes feedstuff industry, and for this reason, new feed resource is actively being found and explored in countries in the world all.Under fermented feed refers to Artificial Control, microorganism is by the metabolic activity of self, ANFs in feedstuff is decomposed, synthesis or conversion, thus produce more can by the nutrient of livestock and poultry absorption and digestion and the feed of nonhazardous, its significant effect causes extensive concern, wherein, complex micro organism fungicide (EM bacterium) is at home and abroad had as fermentation with additives feed and applies very widely, research finds that EM bacterium can improve the conversion ratio of pig feed, reduce diarrhea rate, improve swine product quality, the immunologic function of remarkable enhancing pig, improve resistance against diseases, in daily grain of chicken, add probiotics can improve the laying rate of laying breed, growth performance and immunity.But simple microorganism formulation feed addictive is due to complicated, the easy inactivation in bacterial classification source, the direction of various bacterial strain combination, screening, cultivation is different with method, qualification is difficult to fermented feed quality, and due to the level of production, animal species and feed manufacturing, store, the difference of condition element of feeding, the effect of fermentative feedstuff of microbe differs greatly.Spirulina and chlorella are as unicellular micro-algae, not only be rich in high-quality single cell protein (in cell, protein content is up to more than 65%), and be rich in unrighted acid, vitamin, several mineral materials and pigment etc., security is good and have no side effect, and improving livestock and poultry immunity of organisms, improves the quality of meat and egg and has unique effect.Therefore, unicellular micro-algae, with the nutritional labeling of its uniqueness and physiological activity, presents tempting glamour at the development field of animal feed and feed addictive.But at present mainly with direct, the form that algae powder makes mixed feed is added as the exploitation of feed addictive to spirulina or chlorella, and being used for the production of aquatic animal feed, production development spirulina and chlorella being used for livestock and poultry breeding industry feed addictive is very few.Therefore, develop one and be applicable to the feed addictive prescription being rich in high-quality protein and active microbial inoculum that livestock and poultry (particularly pig and chicken) cultivate and production and processing technology, the production realizing green cultivation and pollution-free food is had very important significance.
Summary of the invention
Technical problem to be solved by this invention, in view of the problem existing for prior art, a kind of phycomycete composite fermented feed additive and preparation method thereof is provided, to carry out science composite by adding spirulina or chlorella and other auxiliary material and complex micro organism fungicide for phycomycete composite fermented feed additive of the present invention, carry out three grade fermemtation, multiple probio quantity and hydrolysis activity in additive for farm animal feed can be improved, increase total protein, the content of the nutritional labelings such as total fat, improve the livestock and poultry animal especially immunity of piglet and the utilization rate of feed, reduce the use of antibiotic product.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of phycomycete composite fermented feed additive, the component containing following weight portion: 10-40 part algae slurry, 5-8 part molasses, 5-8 part residue of soya, 25-35 part yeast paste, 15-30 part dregs of beans, 10-30 part complex microorganism bacteria suspension, 0.01-0.12 part cellulase, 95-182 part water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) algae powder (spirulina powder or chlorella algae powder) and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 10-40 weight portion step 1 is taken) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzyme, mix, carry out broken wall treatment, temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 5-8 weight portion residue of soya, add 20-32 weight parts water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25-35 weight portion yeast paste, mix, fermented and cultured, 28-34 DEG C, 60-100rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 15-30 weight portion dregs of beans, add 75-150 weight parts water, mix, infiltrate, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratio, cut-in quality mark is in the molasses water solution of 5-8%, fermented and cultured, 30-34 DEG C, 200-300rpm stir, and ventilate continuously according to ventilation ratio 0.3-0.5vvm, cultivate 20-24h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 10-30 weight portion step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5-8 weight portion molasses mix, fermented and cultured, 28-34 DEG C of cultivation, and 60-100rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 32-34 DEG C, 60-100rpm stirs, fermentation time is 8-10d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.1-0.3vvm), obtains described phycomycete composite fermented feed additive.
In the step of above-mentioned preparation method, described residue of soya is the residue after the high-salt fermentation sauce wine with dregs squeezing of soy sauce factory; Described yeast paste comes from the 6-7 generation discarded saccharomyces cerevisiae mud produced in beer fermentation process, and water content is mass fraction 75-80%; Described complex micro organism fungicide, is market finished product purchased from victory bio tech ltd, Shanghai three, forms primarily of single bacterial strain compound criteria such as photosynthetic bacteria, lactic acid bacteria, bacillus, Bifidobacterium, saccharomycete.
Phycomycete composite fermented feed additive of the present invention is taked method of directly throwing something and feeding and/or method nursing livestock and poultry of indirectly throwing something and feeding; Directly to throw something and feed method, directly throw something and feed according to the mass fraction 10-15% of livestock and poultry diet total amount and other feed by phycomycete composite fermented feed additive of the present invention simultaneously; Indirectly throw something and feed method, mix with livestock and poultry diet in advance by phycomycete composite fermented feed additive of the present invention, mixer stirs, and throws something and feeds, can use 2-3d after a spice after placing 24h.
The invention has the beneficial effects as follows:
1) nutritious, good absorbing effect: product phycomycete composite fermented feed additive of the present invention with the addition of spirulina or chlorella, containing abundant nutrition; For unicellular micro-algae spirulina, have " optimal high protein source " good reputation, the 60-70% that its protein content weighs up to stem cell, amino acid composition rationally, containing more than the 18 kinds necessary amino acid of animal body, ratio close to FAO, and containing the various bioactivators such as spirulina polysaccharide, unrighted acid, the nutritional need of animal can not only be met, remarkable promotion growth of animal, improve product quality and improve the reproductive performance of animal, and the immunologic function of body can be strengthened, significantly improve the survival rate of cultivated animals; Current spirulina mainly with functional health care product form for specific crowd provide albumen, trophic factors etc. supplement; Spirulina is mainly used in the aquatic livestocks such as fish, Shrimp waste, shellfish as the application of feed addictive, application form mainly contains two kinds: a kind of occupation mode is is directly thrown something and fed as food organisms by micro-algae, another kind of occupation mode is then make the dry algae powder processed of algae, is directly used as a kind of raw material of the aquatic livestock mixed feeds such as livestock and poultry and fish, shrimp, crab, shellfish, ginseng or functional feedstuff additive; Spirulina and the composite fermentation of microbial bacterial agent are used for the technology of livestock and poultry animal feed by the present invention, and there is not been reported; The present invention uses after spirulina or chlorella and the composite fermentation of microbial bacterial agent, compensate for the deficiencies in the prior art, makes algae reach the effect of effective broken wall on the one hand, can promote the stripping of frustule active ingredient; On the other hand by the sweat of composite bacteria agent capable, the macromolecular substances in algae can be degraded, be conducive to the absorption of animal to nutrition and functional component; The cellulase that the present invention uses carries out broken wall treatment to algae slurry, is more conducive to the absorption of algae slurry Middle nutrition composition; Molasses used in the present invention, are mainly used in the carbon source of microbial bacterial agent growth, also can improve the palatability of feed simultaneously; Residue of soya used in the present invention, containing multiple moulds such as aspergillus, after again cultivating, aspergillus can the hydrolase such as secreting amylase, protease, simultaneously in residue of soya also containing several amino acids, nutritious; Yeast paste used in the present invention comes from the waste gas brewer's yeast in beer fermentation process, yeast cells itself is containing high-quality protein, can reach aid digestion simultaneously, improve the effect of animal intestinal function, while refuse higher value application, increase the utilization rate of feed.
2) product characteristics are stablized, easy to use: phycomycete composite fermented feed additive of the present invention, compared with microbial bacterial agent feed addictive simple at present, due to algae powder, residue of soya, again through three grade fermemtation process after yeast pastes etc. are composite, simple microorganism formulation feed addictive can be overcome due to bacterial classification source complexity, easy inactivation, various bacterial strain combination, screening, the direction of cultivating is different with method, qualification is difficult to fermented feed quality, and due to the level of production, animal species and feed manufacturing, store, the difference of condition element of feeding, the shortcoming that the effect of fermentative feedstuff of microbe differs greatly, products obtained therefrom proterties is stablized, easy to use.
3) phycomycete composite fermented feed additive of the present invention is owing to adopting scientific formula and microbial bacterial agent mixed culture fermentation, hydrolase A wide selection of colours and designs in products obtained therefrom, enzymatic activity are high, after using as feed addictive, can effectively improve animal and fowl fodder conversion ratio, improve livestock and poultry animal intestinal health.
4) residue of soya in the formulation auxiliaries that adopts of phycomycete composite fermented feed additive of the present invention, yeast paste all belong to plant produced workshop leftover bits and pieces, with low cost, after the technology of the present invention re-uses, can realize the object that changing rejected material to useful resource increment utilizes, the utilization for factory's leftover bits and pieces provides a new approach; Simultaneously by fermentation technique of the present invention, the feature of the growth metabolism process secretion hydrolase of mould and yeast in residue of soya can be utilized, increase the activity of hydrolase in feed, the conversion ratio of animal and fowl fodder is provided.
Accompanying drawing explanation
Fig. 1 is preparation method's procedure chart that the present invention prepares phycomycete composite fermented feed additive.
Detailed description of the invention
A kind of phycomycete composite fermented feed additive, the component containing following weight portion: 10-40 part algae slurry, 5-8 part molasses, 5-8 part residue of soya, 25-35 part yeast paste, 15-30 part dregs of beans, 10-30 part complex microorganism bacteria suspension, 0.01-0.12 part cellulase, 95-182 part water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) algae powder (spirulina powder or chlorella algae powder) and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 10-40 weight portion step 1 is taken) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzyme, mix, carry out broken wall treatment, temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 5-8 weight portion residue of soya, add 20-32 weight parts water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25-35 weight portion yeast paste, mix, fermented and cultured, 28-34 DEG C, 60-100rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 15-30 weight portion dregs of beans, add 75-150 weight parts water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratio, cut-in quality mark is in the molasses water solution of 5-8%, fermented and cultured, 30-34 DEG C, 200-300rpm stir, and ventilate continuously according to ventilation ratio 0.3-0.5vvm, cultivate 20-24h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 10-30 weight portion step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5-8 weight portion molasses mix, fermented and cultured, 28-34 DEG C of cultivation, and 60-100rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 32-34 DEG C, 60-100rpm stirs, fermentation time is 8-10d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.1-0.3vvm), obtains described phycomycete composite fermented feed additive.
Fig. 1 illustrates the process of the preparation method of above-mentioned phycomycete composite fermented feed additive.
Kilogram corresponding with embodiment of above-described weight portion.
Embodiment 1
A kind of phycomycete composite fermented feed additive, contains: 10kg algae slurry, 5kg molasses, 5kg residue of soya, 25kg yeast paste, 25kg dregs of beans, 10kg complex microorganism bacteria suspension, 0.01kg cellulase, 145kg water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) spirulina powder and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 10kg step 1 is taken) algae that obtains slurry and 0.01kg cellulase, mix, carry out broken wall treatment, temperature is 40 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 5kg residue of soya, add 20kg water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25kg yeast paste, mix, fermented and cultured, 28 DEG C, 60rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 25kg dregs of beans, add 125kg water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8% ratio, cut-in quality mark is in the molasses water solution of 5%, fermented and cultured, 30 DEG C, 200rpm stir, ventilate continuously according to ventilation ratio 0.3vvm, cultivate 20h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 10kg step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5kg molasses mix, fermented and cultured, 28 DEG C of cultivations, and 60rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 32 DEG C, 60rpm stirs, fermentation time is 8d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.1vvm), obtains described phycomycete composite fermented feed additive.
Embodiment 2
A kind of phycomycete composite fermented feed additive, contains: 15kg algae slurry, 8kg molasses, 8kg residue of soya, 35kg yeast paste, 30kg dregs of beans, 15kg complex microorganism bacteria suspension, 0.045kg cellulase, 182kg water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) chlorella algae powder and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 15kg step 1 is taken) algae that obtains slurry and 0.045kg cellulase, mix, carry out broken wall treatment, temperature is 45 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 8kg residue of soya, add 32kg water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 35kg yeast paste, mix, fermented and cultured, 34 DEG C, 100rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 30kg dregs of beans, add 150kg water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 10% ratio, cut-in quality mark is in the molasses water solution of 8%, fermented and cultured, 34 DEG C, 300rpm stir, ventilate continuously according to ventilation ratio 0.5vvm, cultivate 24h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 15kg step 6 that obtain) the complex microorganism bacteria suspension that obtains and 8kg molasses mix, fermented and cultured, 34 DEG C of cultivations, and 100rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 34 DEG C, 100rpm stirs, fermentation time is 10d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.3vvm), obtains described phycomycete composite fermented feed additive.
Embodiment 3
A kind of phycomycete composite fermented feed additive, contains: 12kg algae slurry, 7kg molasses, 7kg residue of soya, 30kg yeast paste, 27kg dregs of beans, 12kg complex microorganism bacteria suspension, 0.024kg cellulase, 163kg water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) spirulina powder and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 12kg step 1 is taken) algae that obtains slurry and 0.024kg cellulase, mix, carry out broken wall treatment, temperature is 42 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 7kg residue of soya, add 28kg water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 30kg yeast paste, mix, fermented and cultured, 30 DEG C, 80rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 27kg dregs of beans, add 135kg water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 9% ratio, cut-in quality mark is in the molasses water solution of 7%, fermented and cultured, 32 DEG C, 250rpm stir, ventilate continuously according to ventilation ratio 0.4vvm, cultivate 22h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 12kg step 6 that obtain) the complex microorganism bacteria suspension that obtains and 57kg molasses mix, fermented and cultured, 30 DEG C of cultivations, and 80rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 33 DEG C, 80rpm stirs, fermentation time is 9d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.2vvm), obtains described phycomycete composite fermented feed additive.
Embodiment 4
A kind of phycomycete composite fermented feed additive, contains: 40kg algae slurry, 5kg molasses, 5kg residue of soya, 25kg yeast paste, 15kg dregs of beans, 30kg complex microorganism bacteria suspension, 0.12kg cellulase, 95kg water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) spirulina powder and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 40kg step 1 is taken) algae that obtains slurry and 0.12kg cellulase, mix, carry out broken wall treatment, temperature is 40 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 5kg residue of soya, add 20kg water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25kg yeast paste, mix, fermented and cultured, 28 DEG C, 60rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 15kg dregs of beans, add 75kg water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8% ratio, cut-in quality mark is in the molasses water solution of 5%, fermented and cultured, 30 DEG C, 200rpm stir, ventilate continuously according to ventilation ratio 0.3vvm, cultivate 20h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 30kg step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5kg molasses mix, fermented and cultured, 28 DEG C of cultivations, and 60rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 32 DEG C, 60rpm stirs, fermentation time is 8d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.1vvm), obtains described phycomycete composite fermented feed additive.
Embodiment 5
A kind of phycomycete composite fermented feed additive, contains: 35kg algae slurry, 5kg molasses, 5kg residue of soya, 25kg yeast paste, 15kg dregs of beans, 25kg complex microorganism bacteria suspension, 0.07kg cellulase, 95kg water.
Prepare a preparation method for phycomycete composite fermented feed additive, comprise the following steps:
1) chlorella powder and water are modulated according to the ratio of 1:10, obtain algae slurry;
2) 35kg step 1 is taken) algae that obtains slurry and 0.01kg cellulase, mix, carry out broken wall treatment, temperature is 40 DEG C, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae and starches;
3) take 5kg residue of soya, add 20kg water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25kg yeast paste, mix, fermented and cultured, 28 DEG C, 60rpm, Continuous Cultivation 24h, obtain mixture I;
5) take 15kg dregs of beans, add 75kg water, mix, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8% ratio, cut-in quality mark is in the molasses water solution of 5%, fermented and cultured, 30 DEG C, 200rpm stir, ventilate continuously according to ventilation ratio 0.3vvm, cultivate 20h, obtain complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 25kg step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5kg molasses mix, fermented and cultured, 28 DEG C of cultivations, and 60rpm rotating speed cultivates 24h, obtains mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) mixture II that obtains mixes, fermented and cultured, temperature is 32 DEG C, 60rpm stirs, fermentation time is 8d, batch (-type) ventilation (every 12h ventilation 30min, ventilation ratio is 0.1vvm), obtains described phycomycete composite fermented feed additive.
The product feature of embodiment 6 phycomycete composite fermented feed additive of the present invention
Phycomycete composite feed additive prepared by the present invention is light brown, has the semi-solid products of algae powder fragranced, and moisture is mass fraction 55-60%, pH 5.5-6.0.In product, beneficial bacterium sum (cfu/mL) is about 1.6 × l0
10, wherein saccharomycete 8.9 × l0
7, lactic acid bacteria 6.8 × l0
9, photosynthetic bacteria 8.4 × l0
9, Bifidobacterium 7.4 × l0
6, bacillus 4.5 × l0
6; The kind of the hydrolase detected in product and enzyme activity unit (U/mL) are respectively: cellulase 30.2, AMS 1926.6, beta amylase 702, acid protease 402.52, neutral proteinase 147.44, alkali protease 100.36; In product, total protein content (mass ratio) is 32.1%, total fat 5.7%, coarse ash 8.2%, phosphorus 0.3%, calcium 0.56%.
Table 1 phycomycete composite feed additive product composition is analyzed
According to the data that phycomycete composite feed additive product composition in table 1 is analyzed, in phycomycete composite feed additive product, the content of the nutriment such as total protein, total fat, phosphorus, calcium is all considerably beyond the minimum that national standard requires, is also better than other a lot of fermented feeds.Such as, Li Long etc. research composite probiotic fermented feed in crude protein content be 26.13%; In the fermentation straw feed of the research such as Bai Xiaojun, protein content is 15.72%; It is 24.61% that the multiple bacteria compound fermentation stalk of the research such as Liao Xueyi produces protein content in protein feeds.
Enzyme activity unit in table 2 phycomycete composite feed additive finished product
The scholars such as Ren Jian detect the enzyme activity in the Xiang Hua biological feedstuff Co., Ltd CHE Feed Sample of Tianjin, find that alpha-amylase activity is 5.6352 μ g/ (gmin), beta amylase vigor is 1.0931 μ g/ (gmin), acid protease activity is 5.203 μ g/ (gmin), neutral protease vigor is 2.9754 μ g/ (gmin), compare after unit conversion, in this phycomycete composite feed additive product, enzyme activity all reaches even higher than the enzyme activity measured in above research, high enzymatic activity is for the conversion ratio improving feed, promote that the overall absorption of body to nutritional labeling has important meaning.
The method of the said goods index detection method with reference to national standard detection method, the analytical method reference as about product composition: total protein content measures to measure according to standard GB/T 5009.5-2010, total lipid content and to measure according to standard GB/T/T6438-2007, phosphorus detection according to standard GB/T/T6437-2002, determination of calcium content according to standard GB/T/T 6436-2002 according to standard GB/T/T22223-2008, coarse ash.
In the hydrolysis activity detection method of phycomycete composite feed additive prepared by the present invention, enzyme unit definition alive is as follows:
Cellulase activity unit definition: at 50 DEG C, under corresponding pH value enzyme needed for hydrolysis generation 1 μ g glucose per minute amount be defined as a unit of activity.Assay method adopts CMC method.
Diastatic activity unit definition: at 40 DEG C, the enzyme amount that under corresponding pH value, hydrolyzed starch per minute produces needed for 1 μ g maltose sugar is defined as 1 unit of enzyme activity.Assay method is according to standard GB/T 8275-2009.
Protease activity unit definition: at 40 DEG C, the enzyme amount that under corresponding pH value, hydrolyzed casein per minute produces needed for 1 μ g tyrosine is defined as 1 unit of enzyme activity.Assay method adopts Folin reagent reducing process.
Embodiment 7
Phycomycete composite feed additive of the present invention is fed piglet, concrete feeding method is: in weanling pig feed, add the phycomycete composite feed additive of the present invention that mass fraction is 15%, within 1st, feed 5 times, be fed with 0.2 kilogram, the feed of additive at every turn; Arrange simultaneously not containing additive feed as a control group.Experimental result shows: in weanling pig feed, add mass fraction 15% phycomycete composite feed additive of the present invention, can improve a poor appetite, effectively improve the speed of growth of piglet, daily gain is about 10-15%, efficiency of feed utilization improves 6-8% simultaneously, feed intake improves 3-4%, reduces the generation of diarrhea of weaned piglets simultaneously, strengthens body premunition, the piglet of continuous use after 50 days, compared with control group, hair is shinny, and vigor is more prosperous.
Embodiment 8
Phycomycete composite feed additive of the present invention is fed Broiler chicks (1-3 age in week), concrete feeding method is: in Broiler chicks basal diet, add the phycomycete composite feed additive of the present invention that mass fraction is 10-15%, within 1st, feed 5-6 time, the feed total amount being fed with additive every day is 150-300g; Arrange simultaneously not containing additive basal diet group in contrast.Experimental result shows: in broiler diet, add mass fraction 10-15% phycomycete composite feed additive of the present invention, chick daily gain is made to improve 4.8-6.5%, survivability of chicks improves 5.5-6.7%, and the immunity of organisms of chick improves, and can obviously promote that it grows.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a phycomycete composite fermented feed additive, it is characterized in that, the component containing following weight portion: 10-40 part algae slurry, 5-8 part molasses, 5-8 part residue of soya, 25-35 part yeast paste, 15-30 part dregs of beans, 10-30 part complex microorganism bacteria suspension, 0.01-0.12 part cellulase, 95-182 part water.
2. a preparation method for phycomycete composite fermented feed additive, is characterized in that, comprises the following steps:
1) by algae powder and water according to 1: 10 ratio modulate, obtain algae slurry;
2) 10-40 weight portion step 1 is taken) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzyme, mix, carry out broken wall treatment, obtain broken wall treatment raw material algae and starch;
3) take 5-8 weight portion residue of soya, add 20-32 weight parts water, mix, obtain rare residue of soya liquid;
4) by step 3) rare residue of soya liquid of obtaining and 25-35 weight portion yeast paste, mix, fermented and cultured, obtain mixture I;
5) take 15-30 weight portion dregs of beans, add 75-150 weight parts water, mix, infiltrate, obtain rare dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratio, cut-in quality mark is in the molasses water solution of 5-8%, and fermented and cultured, obtains complex microorganism bacteria suspension;
7) by step 5) the rare dregs of beans liquid, the 10-30 weight portion step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5-8 weight portion molasses mix, and fermented and cultured, obtains mixtures II;
8) by step 2) raw material algae slurry, step 4 after the broken wall treatment that obtains) the mixture I that obtains and step 7) mixtures II that obtains mixes, and fermented and cultured, obtains described phycomycete composite fermented feed additive.
3. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 1) in, described algae powder is spirulina powder or chlorella algae powder.
4. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 2) in, the condition of described broken wall treatment is: temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h.
5. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 4) in, the condition of described fermented and cultured is 28-34 DEG C, 60-100rpm, Continuous Cultivation 24h.
6. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 6) in, the condition of described fermented and cultured is: 30-34 DEG C, 200-300rpm stirs, and ventilates continuously according to ventilation ratio 0.3-0.5vvm, cultivates 20-24h.
7. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 7) in, the condition of described fermented and cultured is: 28-34 DEG C of cultivation, and 60-100rpm rotating speed cultivates 24h.
8. the preparation method of phycomycete composite fermented feed additive according to claim 2, is characterized in that, step 8) in, the condition of described fermented and cultured is: temperature is that 32-34 DEG C, 60-100rpm stir, and fermentation time is 8-10d, batch (-type) aerobic fementation.
9. the preparation method of phycomycete composite fermented feed additive according to claim 8, it is characterized in that, the aeration condition of described batch (-type) aerobic fementation is: every 12h ventilates 30min, and ventilation ratio is 0.1-0.3vvm.
10. a phycomycete composite fermented feed additive as claimed in claim 1 is in the application of aquaculture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410660199.0A CN104381607B (en) | 2014-11-18 | 2014-11-18 | A kind of phycomycete composite fermented feed additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410660199.0A CN104381607B (en) | 2014-11-18 | 2014-11-18 | A kind of phycomycete composite fermented feed additive and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104381607A true CN104381607A (en) | 2015-03-04 |
| CN104381607B CN104381607B (en) | 2017-06-13 |
Family
ID=52599657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410660199.0A Active CN104381607B (en) | 2014-11-18 | 2014-11-18 | A kind of phycomycete composite fermented feed additive and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104381607B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105767571A (en) * | 2016-03-03 | 2016-07-20 | 刘自忠 | Chlorella laying-chicken feed and feeding method thereof |
| CN107373164A (en) * | 2016-05-17 | 2017-11-24 | 甘肃德福生物科技有限公司 | A kind of chlorella broiler fodder and feeding method |
| CN108077560A (en) * | 2017-12-26 | 2018-05-29 | 陕西理工大学 | A kind of microbial fermentation organic feed |
| CN110468157A (en) * | 2018-05-11 | 2019-11-19 | 台建生技股份有限公司 | The preparation method of algae broken wall fermented hydrolysate and purposes as bird feed additive |
| CN113016954A (en) * | 2021-04-19 | 2021-06-25 | 山东新希望六和集团有限公司 | Fermented feed capable of improving pork quality and preparation method thereof |
| CN113768036A (en) * | 2021-09-16 | 2021-12-10 | 福州天凯生物科技有限责任公司 | Fermentation method of chlorella |
| CN114158645A (en) * | 2021-12-09 | 2022-03-11 | 丰美(沈阳)生物高新技术产业研究院有限公司 | Algae protein feed and preparation method thereof |
| CN115024428A (en) * | 2022-06-15 | 2022-09-09 | 广州希望饲料有限公司 | Antibiotic-free feed additive capable of improving immunity and intestinal tracts of waterfowls and preparation method thereof |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537466A (en) * | 2003-04-20 | 2004-10-20 | 谢新辞 | Instant spirulina products, and its prepn. method |
| CN1943425A (en) * | 2006-10-23 | 2007-04-11 | 深圳职业技术学院 | Fishy smell removing method for spirulina princeps |
| CN101564089A (en) * | 2009-05-21 | 2009-10-28 | 天津市康好饲料有限公司 | Calcareous invertase used for shrimp feed and production technology thereof |
| CN101912050A (en) * | 2010-07-13 | 2010-12-15 | 大连海洋大学 | Micro-granulated feed for shellfish and production technique thereof |
| CN102106570A (en) * | 2011-01-19 | 2011-06-29 | 广西农垦绿仙生物保健食品有限公司 | Method for breaking walls and removing fishy smell of spirulina |
| CN102286381A (en) * | 2010-06-18 | 2011-12-21 | 中美生物资源股份有限公司 | Method for breaking microalgal cell walls |
| CN102987077A (en) * | 2012-12-26 | 2013-03-27 | 日照超凡生物技术有限公司 | Preparation method of seaweed fermented feed |
| CN103039696A (en) * | 2012-11-07 | 2013-04-17 | 方希修 | Algal biological feed and preparation method thereof |
| CN103393189A (en) * | 2013-08-01 | 2013-11-20 | 江苏沃德铝业有限公司 | Production process method of algae beverage |
| CN103497902A (en) * | 2013-07-20 | 2014-01-08 | 烟台大学 | Cladophora comprehensive utilization technology |
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN103849575A (en) * | 2014-03-05 | 2014-06-11 | 南昌大学 | Production method of single-cell protein |
| CN104026412A (en) * | 2014-06-24 | 2014-09-10 | 上海海洋大学 | Penaeus vannamei feed containing chlorella residue |
| CN103859237B (en) * | 2014-04-02 | 2015-08-19 | 金陵科技学院 | A kind of compound aquatic product micro-capsule open-mouthed bait and preparation method thereof and application |
-
2014
- 2014-11-18 CN CN201410660199.0A patent/CN104381607B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537466A (en) * | 2003-04-20 | 2004-10-20 | 谢新辞 | Instant spirulina products, and its prepn. method |
| CN1943425A (en) * | 2006-10-23 | 2007-04-11 | 深圳职业技术学院 | Fishy smell removing method for spirulina princeps |
| CN101564089A (en) * | 2009-05-21 | 2009-10-28 | 天津市康好饲料有限公司 | Calcareous invertase used for shrimp feed and production technology thereof |
| CN102286381A (en) * | 2010-06-18 | 2011-12-21 | 中美生物资源股份有限公司 | Method for breaking microalgal cell walls |
| CN101912050A (en) * | 2010-07-13 | 2010-12-15 | 大连海洋大学 | Micro-granulated feed for shellfish and production technique thereof |
| CN102106570A (en) * | 2011-01-19 | 2011-06-29 | 广西农垦绿仙生物保健食品有限公司 | Method for breaking walls and removing fishy smell of spirulina |
| CN103039696A (en) * | 2012-11-07 | 2013-04-17 | 方希修 | Algal biological feed and preparation method thereof |
| CN102987077A (en) * | 2012-12-26 | 2013-03-27 | 日照超凡生物技术有限公司 | Preparation method of seaweed fermented feed |
| CN103497902A (en) * | 2013-07-20 | 2014-01-08 | 烟台大学 | Cladophora comprehensive utilization technology |
| CN103393189A (en) * | 2013-08-01 | 2013-11-20 | 江苏沃德铝业有限公司 | Production process method of algae beverage |
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN103849575A (en) * | 2014-03-05 | 2014-06-11 | 南昌大学 | Production method of single-cell protein |
| CN103859237B (en) * | 2014-04-02 | 2015-08-19 | 金陵科技学院 | A kind of compound aquatic product micro-capsule open-mouthed bait and preparation method thereof and application |
| CN104026412A (en) * | 2014-06-24 | 2014-09-10 | 上海海洋大学 | Penaeus vannamei feed containing chlorella residue |
Non-Patent Citations (4)
| Title |
|---|
| 付桂明等: "蓝绿藻营养口服液的研制", 《食品工业科技》 * |
| 张丽君等: "螺旋藻脱腥工艺研究", 《食品与发酵工业》 * |
| 王海平等: "螺旋藻破壁发酵脱腥技术研究", 《江苏农业科技》 * |
| 缪坚人等: "《话说螺旋藻：人类精美的原生态食物》", 30 April 2013, 中国农业出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105767571A (en) * | 2016-03-03 | 2016-07-20 | 刘自忠 | Chlorella laying-chicken feed and feeding method thereof |
| CN107373164A (en) * | 2016-05-17 | 2017-11-24 | 甘肃德福生物科技有限公司 | A kind of chlorella broiler fodder and feeding method |
| CN108077560A (en) * | 2017-12-26 | 2018-05-29 | 陕西理工大学 | A kind of microbial fermentation organic feed |
| CN110468157A (en) * | 2018-05-11 | 2019-11-19 | 台建生技股份有限公司 | The preparation method of algae broken wall fermented hydrolysate and purposes as bird feed additive |
| CN113016954A (en) * | 2021-04-19 | 2021-06-25 | 山东新希望六和集团有限公司 | Fermented feed capable of improving pork quality and preparation method thereof |
| CN113768036A (en) * | 2021-09-16 | 2021-12-10 | 福州天凯生物科技有限责任公司 | Fermentation method of chlorella |
| CN114158645A (en) * | 2021-12-09 | 2022-03-11 | 丰美(沈阳)生物高新技术产业研究院有限公司 | Algae protein feed and preparation method thereof |
| CN114158645B (en) * | 2021-12-09 | 2023-09-26 | 丰美(沈阳)生物高新技术产业研究院有限公司 | Algae protein feed and preparation method thereof |
| CN115024428A (en) * | 2022-06-15 | 2022-09-09 | 广州希望饲料有限公司 | Antibiotic-free feed additive capable of improving immunity and intestinal tracts of waterfowls and preparation method thereof |
| CN115024428B (en) * | 2022-06-15 | 2024-05-24 | 广州希望饲料有限公司 | Antibiotic-free feed additive capable of improving immunity and intestinal tract of waterfowl and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104381607B (en) | 2017-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104381607B (en) | A kind of phycomycete composite fermented feed additive and preparation method thereof | |
| CN103652452B (en) | A kind of prawn biological feedstuff and application thereof | |
| CN104920806A (en) | Bran protein feed preparing method by using mixed bacteria multi-step fermentation | |
| CN105661011A (en) | Functional bio-protein feed ferment and fermented protein feed | |
| CN101999527B (en) | Immune feed additive and preparation method thereof | |
| CN102599351A (en) | Bio-enzyme and microorganism containing compound feed additive and preparation process thereof | |
| CN104106727A (en) | Complete fermented feed and preparing method thereof | |
| CN103829042B (en) | A kind of production method of polyvitmin active protein cassava feed | |
| CN103070292A (en) | Method for producing microecological feed additives for livestocks by using agricultural and sideline products | |
| CN104273378A (en) | New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials | |
| CN106212915B (en) | The method and product of staged fermentation production cattle and sheep complete feed | |
| CN102726594A (en) | High-efficiency multienzyme concentration forage | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN109744365A (en) | A kind of mulberry leaf fermented feed and preparation method thereof | |
| CN101642078A (en) | Ecological raising method of nuisanceless pigs by using enzyme preparations and microecological preparations | |
| CN105379947A (en) | Animal feed and preparation method thereof | |
| CN115067419A (en) | Application of feed additive and preparation method of earthworm fermentation liquor | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN106260589A (en) | A kind of method that recycling wheat bran prepares albumen feedstuff | |
| CN105360057A (en) | Pollution-free ecological pig raising method | |
| CN110050890A (en) | A kind of beef cattle fermenting feed and its preparation method and application method | |
| CN103875977A (en) | Mixed feed for aquaculture | |
| CN103892045A (en) | Livestock and poultry feed produced by sophora japonica solid dregs, and preparation method thereof | |
| CN107319173A (en) | A kind of utilization marc mycoprotein promotes the feed of young goose growth | |
| CN103168914B (en) | Preparation technology of microbial glycolysis active peptide containing haematochrome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201123 Address after: No.111 Xingyuan Road, Yunji Industrial Park, Hengnan County, Hengyang City, Hunan Province Patentee after: Hunan gengtianxia Biotechnology Co., Ltd Address before: 264005, Qingquan Road, Laishan District, Shandong, Yantai, 32 Patentee before: Yantai University |