CN105837324A - Hypsizigus marmoreus liquid strain medium and planting method - Google Patents
Hypsizigus marmoreus liquid strain medium and planting method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The present invention provides a hypsizigus marmoreus liquid strain medium, which comprises the following components by weight: 74-80 parts of glucose, 14-20 parts of soybean flour, 1-4 parts of potassium dihydrogen phosphate, 1-4 parts of magnesium dulfate, 0.05-0.1 part of a defoaming agent, 2-6 parts of a growth substance and 600-700 parts of water. The invention belongs to the field of edible fungi cultivation technique. The liquid strain medium can fast breed the bacteria, has high coverage of strains, improves vigor and disease resistance of the strain, and has good resistance to cobweb disease.
Description
Technical field
The invention belongs to fungus growing technique field, particularly relate to liquid spawn culture medium and the cultural method of a kind of Hypsizygus marmoreus.
Background technology
Hypsizygus marmoreus (Hypsizygus marmoreus (Peck) H.E.Bigelow), has another name called Hypsizygus marmoreus, speckle Hypsizygus marmoreus, Lyophyllum decastes.
Comprising multiple necessary aminoacid in the nutritional labeling of Hypsizygus marmoreus, its lysine and arginine content are higher than common mushrooms, and mouthfeel is good,
There is the effect such as diuresis, spleen invigorating.The production of Hypsizygus marmoreus is all by inoculation in culture medium and then to turn out mycelia, finally receive
Obtain what the production technology of product was carried out, the poor growth of Hypsizygus marmoreus, general use multistage strain breeding of method, i.e. by the (examination of one-level kind
Pipe kind), two grades of kinds (triangular flask kind), three grades of kinds (fermentation tank kind) and produce bottle cultivation obtain.
The production strain of Hypsizygus marmoreus includes solid spawn and liquid spawn.Liquid spawn is with fluid medium, at biological fermentation tank
In, by the edible fungus species of the liquid form that liquid fermentation technology produces, liquid spawn relies on the nutrition absorbing fluid medium
Composition grows.Compared with solid spawn, liquid spawn has: the production of hybrid seeds is fast, energetic, shorten cultivation period, cost reduction etc.
Advantage.
Chinese patent application 200810043506.5 discloses a kind of hypsizigus marmoreus in factory and produces liquid spawn culture medium, and component includes
Soyabean expeller powder, Semen Maydis powder, sucrose, magnesium sulfate, potassium dihydrogen phosphate and defoamer, though this culture medium can realize Hypsizygus marmoreus strain
Breeding, but the spawn activity cultivated is not ideal enough, breeding cycle is longer.
The culture medium for cultivating of existing conventional Hypsizygus marmoreus is made up of following composition: cotton seed hulls, wood flour, Testa oryzae, bean cake, big
Cortex beans, wheat bran, Semen Maydis powder, Calx and fine particle calcium carbonate, wherein cotton seed hulls, wood flour, Testa oryzae and wheat bran are main components.In corn cob
Sugar and crude fiber content higher, wherein sugar content about 50%, coarse-fibred content about 30%, due to nutritious and cost
Relatively low, it is also widely used for the cultivation of edible fungi.
Chinese patent application CN 102432353 discloses the culture medium prescription of a kind of Hypsizygus marmoreus, including wood flour, corn cob, Testa oryzae,
Wheat bran, Semen Maydis powder and cotton seed hulls.Chinese patent application CN 102503633 discloses a kind of Hypsizygus marmoreus compost, including rice bran meal,
Wood flour, wheat bran, corn cob, cotton seed hulls, Gypsum Fibrosum powder and pulverized limestone.
Owing to wood flour containing some oils and fatss and aromatic substance, it is unfavorable for edible mycelial growth and fruit body development, the most generally
Re-use after needing wood flour carries out defat or lengthy fermentation process, cause process cycle prolongation, cost increase.Cotton seed hulls master
The ground such as Xinjiang to be originated from, originate deficienter, expensive, and pesticide residues ratio is more serious, therefore, cotton seed hulls are used for a true Ji
The industrialized production of mushroom does not meets economic and environment-friendly requirement.
Chinese crude drug can produce a large amount of Chinese medicine dreg after extracting, and often goes out of use.The discharge of Chinese medicine dreg is easily caused pollution,
Containing compositions such as Plant fiber, protein and polysaccharide in Chinese medicine dreg.The recycling of Chinese medicine dreg is included at sewage by prior art
Manage, be used as medicine, support the modes such as mushroom.For scleroid Chinese medicine dreg, such as Radix Notoginseng medicinal residues, Radix Salviae Miltiorrhizae decoction dregs etc., due to be difficult to by
Degraded, limits its application, causes realizing making full use of of medicinal residues.
Summary of the invention
For solving liquid spawn culture medium and the dissatisfactory problem of culture medium for cultivating present in prior art, inventor is by big
Liquid spawn culture medium and the culture medium for cultivating composition of Hypsizygus marmoreus are screened and compound by amount test, unforeseeable discovery: logical
Crossing and add a small amount of growth regulator, strain can be made to breed quickly, strain coverage rate is high, improves spawn activity and disease-resistant performance.Based on
Above-mentioned discovery, thus complete the present invention.
The purpose of the present invention will be further described in detail below reflect and description.
The present invention provides the liquid spawn culture medium of a kind of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 74-80
Part, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-4 part, defoamer 0.05-0.1 part, growth regulator 2-6 part
With water 600-700 part.
Preferably, the liquid spawn culture medium of the Hypsizygus marmoreus that the present invention provides, including following component and parts by weight thereof: glucose
78 parts, analysis for soybean powder 16 parts, potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth regulator 4 parts and water 650
Part.
Preferably, described growth regulator is made up of with the weight ratio of 4-6: 1-3 gastrodine and propylene glycol alginate sodium sulfate.
Gastrodine is also called Gastrodine, to methylol benzene-β-D pyranglucoside, its chemical formula is: C13H18O7, No. CAS
For: 62499-27-8.Gastrodine is to extract from the dry root block of orchid Rhizoma Gastrodiae to obtain, and it has the calmest and sleeping
Effect, has mitigation to neurasthenia, insomnia, headache syndromes.Propylene glycol alginate sodium sulfate is also called alginic acid the third fat sodium sulfovinate, its
Chemical formula is: C6H9O7Na, No. CAS is: 9005-38-3.Propylene glycol alginate sodium sulfate has anticoagulation, blood fat reducing, improves microcirculation
Deng effect, it is mainly used in ischemic cerebrovascular, is also used for hyperlipidemia, the disease of cardiovascular system is also had certain curative effect.
The present invention is screened by great many of experiments, finds to add the growth regulator being made up of by certain weight ratio gastrodine and propylene glycol alginate sodium sulfate to
In existing Hypsizygus marmoreus liquid spawn culture medium, the vigor of Hypsizygus marmoreus liquid spawn can be significantly improved, improve strain to fermentation ring
The adaptation ability of border and follow-up planting environment.
It is highly preferred that described growth regulator is made up of by weight 5: 2 gastrodine and propylene glycol alginate sodium sulfate.
Preferably, described defoamer is polyether modified polysiloxan defoaming agent, is more preferably purchased from Heng Xin, the Yantai limited public affairs of chemical science and technology
The THIX-298 of department.
Correspondingly, present invention also offers the preparation method of the liquid spawn culture medium of Hypsizygus marmoreus, comprise the steps: to weigh Portugal
Grape sugar, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator, add water, adds defoamer, stirring and dissolving, sterilizing, obtains
Liquid spawn culture medium to Hypsizygus marmoreus.
Additionally, present invention also offers the cultural method of a kind of Hypsizygus marmoreus, comprise the steps:
S1 prepares liquid spawn culture medium: weigh 74-80 part, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-4
Part and growth regulator 2-6 part, add water 600-700 part, adds defoamer 0.05-0.1 part, stirring and dissolving, obtains liquid spawn training
Support base；
The cultivation of S2 fermentation tank strain: described liquid spawn culture medium is added in fermentation tank, sterilizing, fermentation jar temperature is down to 20-24 DEG C,
Hypsizygus marmoreus strain is inoculated in fermentation tank cultivation, aerobic culture 5-8 days under the conditions of 20-24 DEG C, obtains fermentor liquid strain；
Fermentor liquid strain is after inspection is pollution-free, for the cultivation of subsequent production bottle；
S3 prepares culture medium for cultivating: weigh the corn cob of 40-50 parts by weight, the Fructus Schisandrae Chinensis medicinal residues of 3-8 parts by weight, 10-20 weight
The Radix Salviae Miltiorrhizae decoction dregs of number and the Calx of 0.2-1 parts by weight, add the water that temperature is 8-15 DEG C of total consumption 2/3 while stirring, stir
Mix 60-70min, be subsequently adding the Testa oryzae of 8-12 parts by weight, the soybean cover of 5-10 parts by weight, the wheat of 12-20 parts by weight
Bran and the conch meal of 0.8-2 parts by weight, wet-mixing limit, limit adds remaining water, wet-mixing 30-40min, obtains culture medium for cultivating,
Dress produces bottle, sterilizing；
S4 inoculates described fermentor liquid strain in producing in bottle；
S5 mycelia culture, mycelia is covered with and after after-ripening, uses steamed bread type mycelium stimulation cutter mycelium stimulation fruiting；
S6 fruiting is also gathered, and obtains Hypsizygus marmoreus.
Preferably, described Hypsizygus marmoreus strain is in advance through one-level kind and the cultivation of two grades of kinds, and culture medium can use the routine of this area
Culture medium.It is highly preferred that the liquid spawn culture medium that the present invention can be used to provide cultivates one-level kind and two grades of kinds.
Preferably, the water content of described culture medium for cultivating be 63-65%, pH be 6.0-6.6.
Preferably, the particle diameter of described corn cob is 2-8mm；Described Radix Salviae Miltiorrhizae decoction dregs is crushed to particle diameter after bulking machine is expanded
4-6mm；Described Fructus Schisandrae Chinensis medicinal residues size-reduced to particle diameter be 1-3mm.
Preferably, the temperature of described mycelia culture is 20-22 DEG C, and humidity is 65-75%, and gas concentration lwevel is 1000-3500ppm.
Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae are all the widely used medical materials of Chinese herbal medicine industry, usually use alcohol extraction to obtain active component and are used as medicine, remaining
For medicinal residues.After alcohol extraction, remaining Fructus Schisandrae Chinensis medicinal residues and Radix Salviae Miltiorrhizae decoction dregs are possibly together with compositions such as substantial amounts of cellulose and lignins, in addition
Possibly together with nutritional labelings such as a small amount of protein, polysaccharide, owing to the quality of Radix Salviae Miltiorrhizae is hard, it is difficult to be degraded, thus limit it
Application.The present invention by Radix Salviae Miltiorrhizae decoction dregs is carried out expanded after to be crushed to particle diameter be 4-6mm, destroy the structure of few fibers composition,
Make fibre composition loosely organized and be prone to the utilization that is degraded；Additionally, by size-reduced for Fructus Schisandrae Chinensis medicinal residues be 1-3mm to particle diameter, pass through
To corn cob, Radix Salviae Miltiorrhizae decoction dregs and the control of Fructus Schisandrae Chinensis medicinal residues particle diameter, it is achieved that the optimization of culture medium for cultivating structure, improve cultivation
The alimentation of the permeability of culture medium, beneficially Hypsizygus marmoreus and growth.
Owing to the mass percent of corn cob is high, if particle diameter is too big, then bottle weight can be caused partially light, when causing mycelium stimulation, charge level is uneven
Whole；If particle diameter is the least, then bottle can be caused heavily to lay particular stress on, culture medium for cultivating structure is the finest and close, is unfavorable for the growth of mycelia.Wheat
The quality of bran is loosened, and the elasticity for culture medium provides effectively combination, improves the breathability of mycelia, by the interpolation of wheat bran,
Decreasing the consumption of Testa oryzae, raw-material cost reduces further.The price of wheat bran about 900-1000 yuan/ton, and the price of Testa oryzae
About 2500-3000 yuan/ton, the price of wheat bran about 1500-2000 yuan/ton.
Compared with prior art, the invention has the beneficial effects as follows: by adding a small amount of growth regulator in liquid spawn culture medium,
Strain can be made to breed quickly, and strain coverage rate is high, significantly improves spawn activity and disease-resistant performance, has spider web disease and well support
Anti-ability.The cultural method of the Hypsizygus marmoreus that the present invention provides is simple, and low cost, by liquid spawn culture medium and culture medium for cultivating
Harmful, shorten cultivation period, the Hypsizygus marmoreus yield that cultivation obtains is high, quality is good, in good taste, anti-microbial property is good.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further detail.
The liquid spawn culture medium of embodiment one Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 78 parts, analysis for soybean powder 16 parts,
Potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer THIX-2980.06 part, growth regulator 4 parts and 650 parts of water.Growth regulator
It is made up of with the weight ratio of 5: 2 gastrodine and propylene glycol alginate sodium sulfate.
Preparation method: weigh glucose, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator, adds water, adds defoamer,
Stirring and dissolving, sterilizing, obtain the liquid spawn culture medium of Hypsizygus marmoreus.
The liquid spawn culture medium of embodiment two Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 80 parts, analysis for soybean powder 20 parts,
Potassium dihydrogen phosphate 4 parts, 3 parts of magnesium sulfate, defoamer THIX-298 0.1 part, growth regulator 5 parts and 700 parts of water.Growth regulator by
Gastrodine and propylene glycol alginate sodium sulfate form with the weight ratio of 3: 1.Preparation method is with embodiment one.
The liquid spawn culture medium of embodiment three Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 74 parts, analysis for soybean powder 15 parts,
Potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer THIX-298 0.08 part, growth regulator 3 parts and 600 parts of water.Growth regulator
It is made up of with the weight ratio of 5: 2 gastrodine and propylene glycol alginate sodium sulfate.Preparation method is with embodiment one.
The liquid spawn culture medium of comparative example 1 Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 78 parts, analysis for soybean powder 16 parts,
Potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer THIX-298 0.06 part, growth regulator 4 parts and 650 parts of water.Growth regulator
For gastrodine.Preparation method is with embodiment one.
The liquid spawn culture medium of comparative example 2 Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 78 parts, analysis for soybean powder 16 parts,
Potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer THIX-298 0.06 part, growth regulator 4 parts and 650 parts of water.Growth regulator
For propylene glycol alginate sodium sulfate.Preparation method is with embodiment one.
The liquid spawn culture medium of comparative example 3 Hypsizygus marmoreus
The liquid spawn culture medium of Hypsizygus marmoreus, including following component and parts by weight thereof: glucose 78 parts, analysis for soybean powder 16 parts,
Potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer THIX-298 0.06 part, growth regulator 4 parts and 650 parts of water.Growth regulator
It is made up of with the weight ratio of 1: 1 gastrodine and propylene glycol alginate sodium sulfate.Preparation method is with embodiment one.
The cultivation of embodiment four Hypsizygus marmoreus
The liquid spawn culture medium of preparation embodiment one；
The cultivation of fermentation tank strain: added by the liquid spawn culture medium of embodiment one in fermentation tank, sterilizing, fermentation jar temperature drops
To 22 DEG C, the Hypsizygus marmoreus strain cultivated through two grades of kinds is inoculated in fermentation tank cultivation, aerobic culture 6 days under the conditions of 22 DEG C,
Obtain fermentor liquid strain；The most pollution-free, reach the condition for the cultivation of subsequent production bottle；
Preparation culture medium for cultivating: weigh the corn cob of 45 parts by weight, the Fructus Schisandrae Chinensis medicinal residues of 5 parts by weight, 15 parts by weight
Radix Salviae Miltiorrhizae decoction dregs and the Calx of 0.5 parts by weight, add the water that temperature is 10 DEG C of total consumption 2/3 while stirring, stirs 60min,
It is subsequently adding the Testa oryzae of 10 parts by weight, the soybean cover of 7 parts by weight, the wheat bran of 16 parts by weight and the shellfish of 1.5 parts by weight
Shell powder, wet-mixing limit, limit adds remaining water, wet-mixing 30min, obtains culture medium, bottling, sterilizing；The water content of culture medium is
64%, pH is 6.2.The particle diameter of described corn cob is 2-8mm；Described Radix Salviae Miltiorrhizae decoction dregs is crushed to particle diameter after bulking machine is expanded
4-6mm；Described Fructus Schisandrae Chinensis medicinal residues size-reduced to particle diameter be 1-3mm；
Inoculation Hypsizygus marmoreus strain: using liquid inoculator inoculation, use the culture bottle (making a call to three holes) of 850ml, inoculum concentration is: 20mL/
Bottle, inoculation temperature is 20 DEG C；
Mycelia culture: temperature is 20 DEG C, humidity is 68%, and gas concentration lwevel is 2000～3000ppm, cultivates 24 days mycelia
Covering with bottle, then the after-ripening through 29 days, mycelia transfers khaki to；
Mycelium stimulation: mycelia is covered with and after after-ripening, uses steamed bread type mycelium stimulation cutter mycelium stimulation fruiting, strain central authorities are depressed gently with recessed ware,
Surrounding is scratched simultaneously；
Fruiting is also gathered, and obtains Hypsizygus marmoreus.Every bottle of yield producing bottle is 193.6g, and cap is not deployed, a diameter of 1.5cm,
Lamella, stem white, mushroom shape is neat, and stem is sturdy, tall and straight, and height is 7.3cm, and free from admixture goes mouldy.To in Hypsizygus marmoreus
The content of arsenic, lead, hydrargyrum etc. detects, and meets GB 7096-2014 (national food safety standard edible fungi and goods thereof)
Regulation, safety is good.
The cultivation of comparative example 4 Hypsizygus marmoreus
With differing only in of embodiment four: preparation culture medium for cultivating step in, weigh 10 parts by weight Fructus Schisandrae Chinensis medicinal residues,
The Radix Salviae Miltiorrhizae decoction dregs of 10 parts by weight.That is, the weight ratio of Fructus Schisandrae Chinensis medicinal residues and Radix Salviae Miltiorrhizae decoction dregs is become 1: 1 by 1: 5.
Experiment in cultivation example
The liquid spawn culture medium that Example one, embodiment two, comparative example 1, comparative example 2 and comparative example 3 prepare is pressed respectively
The same terms in embodiment four is inoculated and cultivates, as corresponding test group, and using comparative example 4 as culture medium for cultivating
Comparative example four groups, the culture effect of Hypsizygus marmoreus is compared, result is as shown in table 1.
The cultivation effect of the different condition of culture of table 1
As known from Table 1, the liquid spawn culture medium of present invention offer and culture medium for cultivating are beneficial to the fast-growth of Hypsizygus marmoreus, identical
Under the conditions of, embodiment one group reach produce bottle cultivation condition fermented bacterium cultivation cycle be 6 days, hence it is evident that than comparative example 1 group,
Comparative example 2 groups and comparative example 3 groups are shorter, and the cultivation period producing the full bottle of bottle significantly foreshortens to 25 days, and improve yield,
Product in good taste.The cultivation effect that embodiment is one group is optimal, and therefore, embodiment one is highly preferred embodiment of the present invention.
Additionally, different tests group spider web disease incidence rate under the same conditions is also added up by the present invention, result such as table 2 institute
Show.The sporophore sum of the sporophore sum of spider web disease incidence rate=have scab/all.As known from Table 2, the embodiment of the present invention one is made
The liquid spawn culture medium obtained, for the cultivation of Hypsizygus marmoreus strain, can significantly improve the resistivity sick to spider web, and sickness rate shows
Write and reduce.
The Hypsizygus marmoreus sporophore spider web disease incidence rate of the different cultivation condition of table 2
| Group | Spider web disease incidence rate (%) |
| Embodiment one group | 1.8 |
| Embodiment two groups | 2.3 |
| Comparative example 1 group | 18.9 |
| Comparative example 2 groups | 21.5 |
| Comparative example 3 groups | 12.6 |
| Comparative example 4 groups | 8.0 |
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert the present invention
Be embodied as be confined to these explanations.For general technical staff of the technical field of the invention, without departing from this
On the premise of inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. the liquid spawn culture medium of a Hypsizygus marmoreus, it is characterised in that: include following component and parts by weight thereof: glucose 74-80
Part, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-4 part, defoamer 0.05-0.1 part, growth regulator 2-6
Part and water 600-700 part.
The liquid spawn culture medium of Hypsizygus marmoreus the most according to claim 1, it is characterised in that: include following component and weight portion thereof
Number: glucose 78 parts, analysis for soybean powder 16 parts, potassium dihydrogen phosphate 2 parts, 2 parts of magnesium sulfate, defoamer 0.06 part, growth encourage
Agent 4 parts and 650 parts of water.
The liquid spawn culture medium of Hypsizygus marmoreus the most according to claim 1, it is characterised in that: described growth regulator is by gastrodine and algae
Acid diester sodium forms with the weight ratio of 4-6: 1-3.
The liquid spawn culture medium of Hypsizygus marmoreus the most according to claim 3, it is characterised in that: described growth regulator is by gastrodine and algae
Acid diester sodium is by weight 5: 2 compositions.
The liquid spawn culture medium of Hypsizygus marmoreus the most according to claim 1, it is characterised in that: described defoamer is polyether-modified silicon
Oil defoamer.
The preparation method of the liquid spawn culture medium of Hypsizygus marmoreus the most according to any one of claim 1 to 5, it is characterised in that:
Comprise the steps: to weigh glucose, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator, add water, add defoamer,
Stirring and dissolving, sterilizing, obtain the liquid spawn culture medium of Hypsizygus marmoreus.
7. the cultural method of a Hypsizygus marmoreus, it is characterised in that: comprise the steps:
S1 prepares liquid spawn culture medium: weigh 74-80 part, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 part, magnesium sulfate 1-4
Part and growth regulator 2-6 part, add water 600-700 part, adds defoamer 0.05-0.1 part, stirring and dissolving, obtains liquid spawn training
Support base；
The cultivation of S2 fermentation tank strain: described liquid spawn culture medium is added in fermentation tank, sterilizing, fermentation jar temperature is down to 20-24 DEG C,
Hypsizygus marmoreus strain is inoculated in fermentation tank cultivation, aerobic culture 5-8 days under the conditions of 20-24 DEG C, obtains fermentor liquid strain；
S3 prepares culture medium for cultivating: weigh the corn cob of 40-50 parts by weight, the Fructus Schisandrae Chinensis medicinal residues of 3-8 parts by weight, 10-20 weight
The Radix Salviae Miltiorrhizae decoction dregs of number and the Calx of 0.2-1 parts by weight, add the water that temperature is 8-15 DEG C of total consumption 2/3 while stirring, stir
Mix 60-70min, be subsequently adding the Testa oryzae of 8-12 parts by weight, the soybean cover of 5-10 parts by weight, the wheat of 12-20 parts by weight
Bran and the conch meal of 0.8-2 parts by weight, wet-mixing limit, limit adds remaining water, wet-mixing 30-40min, obtains culture medium for cultivating,
Dress produces bottle, sterilizing；
S4 inoculates described fermentor liquid strain in producing in bottle；
S5 mycelia culture, mycelia is covered with and after after-ripening, uses steamed bread type mycelium stimulation cutter mycelium stimulation fruiting；
S6 fruiting is also gathered, and obtains Hypsizygus marmoreus.
The cultural method of Hypsizygus marmoreus the most according to claim 7, it is characterised in that: the water content of described culture medium for cultivating is
63-65%, pH are 6.0-6.6.
The cultural method of Hypsizygus marmoreus the most according to claim 7, it is characterised in that: the particle diameter of described corn cob is 2-8mm；Institute
Stating Radix Salviae Miltiorrhizae decoction dregs and being crushed to particle diameter after bulking machine is expanded is 4-6mm；Described Fructus Schisandrae Chinensis medicinal residues size-reduced to particle diameter be 1-3mm.
The cultural method of Hypsizygus marmoreus the most according to claim 7, it is characterised in that: the temperature of described mycelia culture is 20-22 DEG C,
Humidity is 65-75%, and gas concentration lwevel is 1000-3500ppm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521500A (en) * | 2019-10-10 | 2019-12-03 | 西充星河生物科技有限公司 | A kind of true pleurotus cornucopiae liquid spawn production technology |
| CN111527992A (en) * | 2020-05-06 | 2020-08-14 | 江苏华绿生物科技股份有限公司 | Culture medium for hypsizigus marmoreus |
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| CN113383677A (en) * | 2021-07-21 | 2021-09-14 | 西充星河生物科技有限公司 | Hypsizigus marmoreus tissue isolation medium and preparation method thereof |
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