CN101292640A - Process of fly louse with noxious ash by using freezing method - Google Patents
Process of fly louse with noxious ash by using freezing method Download PDFInfo
- Publication number
- CN101292640A CN101292640A CNA2008101239372A CN200810123937A CN101292640A CN 101292640 A CN101292640 A CN 101292640A CN A2008101239372 A CNA2008101239372 A CN A2008101239372A CN 200810123937 A CN200810123937 A CN 200810123937A CN 101292640 A CN101292640 A CN 101292640A
- Authority
- CN
- China
- Prior art keywords
- brown rice
- small brown
- rice planthopper
- virus
- planthopper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Catching Or Destruction (AREA)
- Adjustment And Processing Of Grains (AREA)
Abstract
The invention discloses a novel method for treating small brown rice planthopper which carries virus. The essence of the invention leads the moving ability of the small brown rice planthopper to drop temporarily by adopting a refrigeration method so as to start the operation of quantitative capturing. With the recovery of body temperature, the abilities of moving, feeding and virus transmitting of small brown rice planthopper are recovered again; therefore, the purpose for moving small brown rice planthopper rapidly and quantitatively can be achieved. Compared with the original method, the method of the invention is characterized by being more convenient and rapid and is applied to inoculation identification of small brown rice planthopper which transmits virus diseases such as stripe virus of rice, etc.
Description
Technical field
The present invention handles a kind of new method of small brown rice planthopper of carrying virus, belongs to plant disease resistance authenticate technology field.
Background technology
The stripe disease that rice stripe virus (RSV) causes found that all there is generation the back in Korea, Ukraine and China in the Japanese Northeast early than 1897.China from 1962 since this virus is found in the Jiangsu and Zhejiang Provinces, take place at 16 provinces in the whole nation, the THE LOWER YANGTZE VALLEY rice district based on Jiangsu Province still is in the popular phase at present.According to incompletely statistics, only Jiangsu Province's stripe disease generation area in 2004 just reaches 2,700 ten thousand mu, accounts for 79% of Jiangsu Province's rice area, and the expert estimates that stripe disease will be in after 2006 5 years and lay particular stress on occurrence tendency that prevention work is very urgent.
Stripe disease is a kind of viroses of plant of being propagated by small brown rice planthopper, in case just past medical help substantially after morbidity is caused harm, produce at present and go up how employing is controlled the worm diseases prevention, adjusted measures such as broadcasting the phase of planting, but it is big in the worm amount, small brown rice planthopper is with malicious rate height, if, long disease outbreak year part of adult peak phase, both pest control effects are all undesirable.External experience of prevention and treatment shows, is that breeding resistant variety is applied to produce at the most cost-effective method of this disease.Studies show that the qualification result that seedling stage, indoor inoculation was identified is reliable and stable, is the prefered method that resistance is identified, but because to pass the mobility of virus mediator small brown rice planthopper stronger, quantitatively inoculates small brown rice planthopper and be always a complicated operation and loaded down with trivial details work.Thereby, invent a kind of small brown rice planthopper processing method easily and fast, can accelerate small brown rice planthopper greatly and pass the speed that the virus disease inoculation is identified, and then the mirror of quickening disease-resistant variety goes out speed and seed selection process.
Summary of the invention
The objective of the invention is provides a kind of processing to carry the new method of the small brown rice planthopper of virus for the inoculation evaluation of small brown rice planthoppers such as stripe disease biography virus disease.
Method provided by the present invention is by freezing method the locomotivity of small brown rice planthopper temporarily to be descended, quantitatively get the worm operation to carry out, along with the rise of body temperature, small brown rice planthopper recovers to move, get food again and passes malicious ability, and then reaches the purpose of the mobile small brown rice planthopper in fast quantification ground.
In said method, what choose is the small brown rice planthopper that can be used for virus inoculation 2～5 ages, and freezing temperature is-20 ℃, and cooling time is 20 minutes.
The described small brown rice planthopper that is used for handling should be at the container of relative closure before freezing, and it can be to seal glass or circular open plastic containers with nylon gauze, requires to have preferably air flow property to guarantee the small brown rice planthopper survival, can prevent that again small brown rice planthopper from escaping from container simultaneously.
Small brown rice planthopper with the temporary transient devitalization in freezing back, can carry out the quantitative counting operation of similar several rice paddy seeds, being ready to put it into planting of getting ready in advance behind the small brown rice planthopper of capacity needs to identify in the inoculation container of paddy rice seedling, treat that small brown rice planthopper body temperature gos up, mobility is recovered, and can pass the poison inoculation.
The invention provides a kind of processing and carry the new method of the small brown rice planthopper of virus, be named as " process of fly louse with noxious ash by using freezing ".The small brown rice planthopper that this method is more traditional is got worm method etc. and has the following advantages: 1. carry out the processing of the virus inoculation of predetermined quantity with small brown rice planthopper more easily; 2. carry out the processing of virus inoculation faster with small brown rice planthopper; 3. be convenient to choose the virus inoculation in the suitable length of time and use small brown rice planthopper.Thereby can accelerate speed and the accuracy that small brown rice planthopper passes virus disease inoculation evaluation greatly, and then the mirror of quickening disease-resistant variety goes out speed and seed selection process.
Embodiment
Method therefor is conventional method if no special instructions among the following embodiment.
Embodiment 1, with the experiment of the cooling time gradient of malicious small brown rice planthopper
Get 6 dry glass cups, numbering is chosen the small brown rice planthopper of carrying rice stripe virus by a certain percentage and is placed in one, about every glass 100 worm.Be put in simultaneously and under-20 ℃ of conditions, begin freezing in the refrigerator and per 10 minutes of timing is taken out 1 glass, rapidly plant hopper is fallen on blank sheet of paper after the taking-up,, observe also record small brown rice planthopper active state, recovery time and dead borer population with the writing brush number.Freezing small brown rice planthopper useed force educate No. 3, round-grained rice and raise separately, added up dead borer population in second day.
The result shows: freezing 10 minutes, small brown rice planthopper did not go into a coma, and still can beat, and was unfavorable for number worm on paper; But along with cooling time surpasses 30 fens kinds, the vitality of small brown rice planthopper can be influenced, and lethality increases (table 1) with the increase of cooling time.Comprehensive recovery time and lethality are proper with-20 ℃ times freezing about 20 minutes with the freezing processing of malicious small brown rice planthopper as can be known.
The state of the different cooling time small brown rice planthoppers of table 1
Embodiment 2, freezing processing are to passing the influence test of malicious ability with malicious small brown rice planthopper
Force is educated No. 3 seed soaking of round-grained rice, and vernalization is sowed in the glass during to 0.5 leaf; Broadcast seedling 12 strains for every glass, broadcast 6 glasss.From carry rice stripe virus small brown rice planthopper colony by a certain percentage, get about 120, as stated above-20 ℃ under with freezing 20 minutes of small brown rice planthopper, number is got 120 worms, the force that is connected to 1.5 leaf ages is educated on No. 3, the round-grained rice, repeats 3 times.Accurately draw force that 120 worms are connected to 1.5 leaf ages with suction pipe simultaneously and educate on No. 3, the round-grained rice, repeat 3 times.Glass seals with gauze, after 48 hours, small brown rice planthopper is removed.Transplant in the land for growing field crops, note field management.Institute an inquiry plant incidence (the sick level criteria for classifying is with reference to summary of the invention) after 7 days, subsequently once every investigation in 3～6 days, with onset peak period and can reflect that the investigation record data of anti-(sense) characteristic of disease difference serve as that foundation is made the disease resistance evaluation, require to show disease period average temperature and be not less than 20 ℃, the minimum air temperature is not less than 15 ℃.
The freezing small brown rice planthopper of table 2 is to passing the influence of malicious ability
The result shows: the incidence of disease that the small brown rice planthopper of freezing processing inoculation back force is educated No. 3, round-grained rice is lower than not freezing processing, but significance analysis is found the two there was no significant difference, and visible freezing processing is to propagating the not influence of ability of rice stripe virus with malicious small brown rice planthopper.
The present invention mainly serves at stripe disease and carries out disease-resistant variety seed selection work, and the inoculation evaluation that passes virus disease for small brown rice planthoppers such as stripe diseases provides a kind of processing to carry the new method of the small brown rice planthopper of virus.The application of this method can be accelerated small brown rice planthopper greatly and pass speed and the accuracy that the virus disease inoculation is identified, and then the mirror of quickening disease-resistant variety goes out speed and seed selection process.
Above-mentioned enforcement does not limit the present invention in any form.
Claims (1)
1, the new method of the small brown rice planthopper of virus is carried in a kind of processing, it is characterized in that: the locomotivity of small brown rice planthopper is temporarily descended by freezing method, quantitatively get the worm operation to carry out, rise along with body temperature, small brown rice planthopper recovers to move, get food again and passes malicious ability, and then reaches the purpose of the mobile small brown rice planthopper in fast quantification ground.Its key is: what (one) chose is the small brown rice planthopper that can be used for virus inoculation 2～5 ages; (2) freezing temperature is-20 ℃; (3) cooling time is 20 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101239372A CN101292640B (en) | 2008-06-12 | 2008-06-12 | Process of fly louse with noxious ash by using freezing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101239372A CN101292640B (en) | 2008-06-12 | 2008-06-12 | Process of fly louse with noxious ash by using freezing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101292640A true CN101292640A (en) | 2008-10-29 |
| CN101292640B CN101292640B (en) | 2010-06-30 |
Family
ID=40063394
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101239372A Expired - Fee Related CN101292640B (en) | 2008-06-12 | 2008-06-12 | Process of fly louse with noxious ash by using freezing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101292640B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401562B (en) * | 2008-11-13 | 2010-12-08 | 江苏省农业科学院 | Acquiring method for special small brown rice planthopper for inoculating rice black-streaked dwarf virus |
| CN105837324A (en) * | 2016-03-28 | 2016-08-10 | 东莞市合心生物科技有限公司 | Hypsizigus marmoreus liquid strain medium and planting method |
| CN114946569A (en) * | 2021-12-30 | 2022-08-30 | 江苏省农业科学院 | Novel method for improving rice stripe virus infection efficiency through leaf inoculation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425120C (en) * | 2006-11-15 | 2008-10-15 | 江苏省农业科学院 | A method for determining brown strip resistant rice species |
| CN101124900B (en) * | 2007-09-14 | 2010-05-26 | 王华弟 | Survey and sampling device for homoptera insects |
-
2008
- 2008-06-12 CN CN2008101239372A patent/CN101292640B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401562B (en) * | 2008-11-13 | 2010-12-08 | 江苏省农业科学院 | Acquiring method for special small brown rice planthopper for inoculating rice black-streaked dwarf virus |
| CN105837324A (en) * | 2016-03-28 | 2016-08-10 | 东莞市合心生物科技有限公司 | Hypsizigus marmoreus liquid strain medium and planting method |
| CN105837324B (en) * | 2016-03-28 | 2017-04-12 | 东莞市合心生物科技有限公司 | Hypsizigus marmoreus liquid strain medium and planting method |
| CN114946569A (en) * | 2021-12-30 | 2022-08-30 | 江苏省农业科学院 | Novel method for improving rice stripe virus infection efficiency through leaf inoculation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101292640B (en) | 2010-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101401562B (en) | Acquiring method for special small brown rice planthopper for inoculating rice black-streaked dwarf virus | |
| CN100446655C (en) | Sprout period authenticating method for tobacco black shank fastness | |
| Liu et al. | Progress and constraints of dry direct-seeded rice in China | |
| CN102870743A (en) | Method for preserving rice black-streaked dwarf virus indoor living bodies | |
| CN104303866A (en) | Method for identifying and evaluating wheat scab expansion resistance | |
| CN101292640B (en) | Process of fly louse with noxious ash by using freezing method | |
| CN100425117C (en) | Sprout period authenticating method for tobacco mosaic resistantance | |
| CN102239779B (en) | Method for quickly identifying resistance of Chinese cabbage germplasm to black-spot disease | |
| CN111328496B (en) | Method for measuring sunflower seed vitality | |
| CN101816273A (en) | Identification method for indoor inoculation of anti-black-streaked dwarf disease of rice varieties | |
| CN1802896A (en) | Cotton breeding artificial isolation chamber anti- cotton aphid authentication method | |
| CN103931563B (en) | Transgenic pest-resistant rice is to predator Hylyphantes graminicolum method for evaluating safety | |
| CN102948336A (en) | Method for inoculating tomatoes with tomato yellow leaf curl viruses | |
| Honěk et al. | The induction of secondary seed dormancy by oxygen deficiency in a barnyard grass Echinochloa crus-galli | |
| Co et al. | Evaluation of smut inoculation techniques in sugarcane seedlings | |
| CN101810073B (en) | Hydro-priming method for Chinese pine seeds | |
| CN104152533B (en) | One method growing tobacco seedling phase Rapid identification black shank fastness | |
| Marshall | THE INCIDENCE OF CERTAIN SEED‐BORNE DISEASES IN COMMERCIAL SEED SAMPLES: I. LOOSE SMUT OF BARLEY, USTILAGO NUDA (JENS.) ROSTR. | |
| Mala et al. | Effective spore density of Glomus mosseae, arbuscular mycorrhiza (AM), for inoculation of rooted cuttings of black pepper (Piper nigrum Linn.) | |
| Ruza et al. | The impact of sowing time on sugar content and snow mould development in winter wheat | |
| CN105075698A (en) | Tobacco black shank resistance single plant identification method | |
| CN111334584A (en) | Method for predicting SPCSV (Spot Summit syndrome Virus) toxicity rate of sweet potato seeds | |
| CN109287191A (en) | A kind of method of cotton seeds Salt-Tolerance Identification | |
| Parveen et al. | Effect of planting time on sheath blight disease of rice in Bangladesh | |
| CUI et al. | Hatching and development of maize cyst nematode Heteroderazeae infecting different plant hosts1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100630 Termination date: 20200612 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |