CN109259202A - The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn - Google Patents
The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn Download PDFInfo
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- CN109259202A CN109259202A CN201811205200.5A CN201811205200A CN109259202A CN 109259202 A CN109259202 A CN 109259202A CN 201811205200 A CN201811205200 A CN 201811205200A CN 109259202 A CN109259202 A CN 109259202A
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- tremella
- parts
- corn
- chinese yam
- solid state
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of methods for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn, and using rice, corn, Chinese yam as primary raw material, calcium carbonate is auxiliary material, it is mixed after sterilizing with tremella, it divides on solid state fermentation bed, carries out solid state fermentation, obtained fermentation material is blood sugar reducing food.Of the invention every gram of blood sugar reducing food contains 2~30mg of polysaccharide, 2~10mg of saponin(e, blood glucose can be significantly reduced in the blood sugar reducing food, it is a kind of pure natural preparation, the not only quick, significant effect compared with existing hypoglycemic medicine, and not will lead to hypoglycemia after taking.
Description
Technical field
The present invention relates to a kind of methods for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn, belong to biology
Field of engineering technology.
Background technique
It is especially high with China's expanding economy, the raising of living standards of the people and the variation of people's dietary structure
Rouge dietary int ake increases, and the disease incidence of the " three high " disease such as hypertension, hyperlipidemia and hyperglycemia rises year by year.Control and prevention
" three high ", although drug effect is preferable, side effect is big, price is relatively high, and not each patient can receive, therefore develops
The food that curative effect is better, has no toxic side effect has good development prospect.
Chinese yam is the cultivar that underground meat stem tuber can be formed in potato preparatory course Dioscorea, annual or perennial prehensile rattan
This plant, alias Chinese yam, potato damage, RHIIZOMA DIOSCOREAE from Henan of China etc..Chinese yam is a kind of highly efficient and productive industrial crops of dietotherapeutic, drying to be used as medicine
For tonic invigorator, there is auxiliary curative effect to weakness, chronic enteritis, diabetes etc..Chinese yam is rich in protein, amino acid, starch etc.
Nutriment and polysaccharide, dioscin (steroid saponin), vitamin, allantoin, dopamine, Chinese yam alkali, polyphenol oxidase etc.
Bioactive ingredients.The initial eating method of Chinese yam is long term storage after removing the peel, dry, being milled, and yam flour is usually passed through boiling
It is made into various food or directly stews Chinese yam at soup and eat.In recent years, people are to Chinese yam with the progress of science and technology
Exploitation also gradually to in-depth development, there are many report in relation to Chinese yam product R & D: Chinese yam convection drying is made
Quan Fen, fine powder, Chinese yam are directly beaten, series beverage are made, and ourishing rice flour is made in Chinese yam starch, by Chinese yam and pure milk, rice
Equal composite fermentations are at yam Yogurt and yam wine etc..But unstable quality, and Chinese yam also has the general character of Chinese medicine, slow effect.
Mountain the effective elements of the medicine is directly extracted to exploitation and is increasingly becoming Chinese yam development and utilization at food with health care function
New approaches.
Tremella (Tremella fuciformis) is Basidiomycota, Heterobasidiomycetes guiding principle, Tremellales, Tremellaceae, tremella
Belong to, tremella is the invigorant that China has long enjoyed a good reputation.Tremella, which has, " grows positive kidney tonifying, moistening lung to arrest cough, stomach function regulating ease constipation, QI invigorating and blood, cerebrum tonifying
Refresh oneself, strong body strengthening tendons, skin-softening beauty, promote longevity " function.Modern medicine study shows that tremella main active is tremella
Polysaccharide is made of acid heteroglycan, neutral heteropolysaccharide., and there is enhancing to be immunized, antitumor, reducing blood lipid, and anti-inflammatory is anti-oxidant etc.
Effect.Tremella polysaccharides are divided into fruitbody polysaccharide, mycelium polysaccharides according to source difference, and tremella embraces sub- polysaccharide and ferments extracellular more
Sugar；Water-soluble polysaccharide and alkali-soluble polysaccharide can be divided into according to dissolubility, be not all that neutral polysaccharide and acidity are miscellaneous according to acid-base property point
Polysaccharide.Tremella polysaccharides have enhancing it is immune, it is antitumor with inflammation, anti-aging and hypoglycemic and other effects.It has disclosed at present
How report in relation to tremella mainly obtains tremella strain, the cultivation of tremella or the extraction of tremella polysaccharides.Do not utilize silver
The unfamiliar to the ear report for producing the food containing saponin(e and polysaccharide, and be all laboratory scale, can technology be used to industrialize
It can't determine.
Summary of the invention
The solid state transformed Chinese yam of tremella and corn system are utilized present invention aims to solve the deficiencies of the prior art, and provides a kind of a kind of
The method of standby blood sugar reducing food.
Technical solution
Tremella bacterium is expanded culture technique and combined with solid-state bioconversion by the present inventor, using tremella as strain, Chinese yam, jade
Rice and rice are primary raw material, are combined using fermentation with conversion, produce the food with function of blood sugar reduction.Concrete scheme is such as
Under:
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of tremella and corn, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) tremella strain is inoculated in potato glucose slant medium
In, after cultivating 8-10d at 20-30 DEG C, tremella slant strains, 10 DEG C of preservations are made；
(2) tremella bacterium is cultivated: tremella inclined-plane made from step (1) being cut into the fungus block of 10 × 10mm of 7-10 block or so, is chosen
It takes 1 piece to be inoculated into tremella bacterium solid seed culture medium, after cultivating 35-40d at 25-30 DEG C, tremella bacterium solid spawn is made；Its
In, tremella bacterium solid seed culture medium composition are as follows: 30-60 parts of corn, 10-30 parts of wheat bran, 13-38 parts of maize peel, yeast
1-10 parts of medicinal extract, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, and then tremella bacterium strain made from inoculation step (2), is mixed
It is divided after even on solid state fermentation bed, thickness 6-20cm, width 60-120cm, after cultivating 3-15d in solid state fermentation culturing room,
It is dry to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 100 parts of rice, 30-60 parts of corn, Chinese yam 20-60
Part, 0.5-5 parts of calcium carbonate, 60-150 parts of water.
Further, in step (1), potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, fine jade
20 grams of rouge, water 1000mL.
Further, in step (3), every 100g solid fermentation inoculation of medium 1-20g tremella bacterium solid spawn.
Further, in step (3), it is small that corn and water the preparation method of the solid fermentation culture medium: are mixed into immersion 24
Shi Hou is mixed with rice, Chinese yam, adds calcium carbonate, after mixing 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Further, in step (3), the condition of solid state fermentation culture are as follows: 20-35 DEG C of temperature, ventilatory capacity 0.05-0.5:
1v/v/m。
The utility model has the advantages that the present invention prepares blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn, and with corn,
Rice and Chinese yam be matrix solid medium in add calcium carbonate, significantly improve containing for tremella polysaccharides and saponin(e in product
Amount, makes polysaccharide and saponin content increase separately 20% and 25%, to significantly enhance the function of blood sugar reduction of product；Of the invention
Blood sugar reducing food is a kind of pure natural preparation, and every gram contains 2~30mg of polysaccharide, 2~10mg of saponin(e, compared with existing hypoglycemic medicine
Not only quick, significant effect, and not will lead to hypoglycemia after taking, it is without any side effects.
Detailed description of the invention
The change of blood sugar curve graph of mouse after the blood sugar reducing food that Fig. 1 is feeding addition embodiment 1-3.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples.
Embodiment 1
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of tremella and corn, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) tremella strain is inoculated in potato glucose slant medium
In, after cultivating 10d at 20 DEG C, tremella slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) tremella bacterium is cultivated: tremella slant strains made from step (1) being cut into the fungus block of 8 pieces of 10 × 10mm or so, are chosen
It takes 1 piece to be inoculated into tremella bacterium solid seed culture medium, after cultivating 40d at 25 DEG C, tremella bacterium solid spawn is made；Wherein, silver-colored
Ear bacterium solid seed culture medium composition are as follows: 30 parts of corn, 30 parts of wheat bran, 38 parts of maize peel, 10 parts of yeast extract, di(2-ethylhexyl)phosphate
4 parts of hydrogen potassium, 2 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then tremella bacterium solid bacterium made from inoculation step (2)
Kind (every 100g solid fermentation inoculation of medium 1g tremella bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly
6cm, width 120cm, under conditions of 35 DEG C of temperature, ventilatory capacity are 0.5:1v/v/m in solid state fermentation culturing room after culture 3d,
90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 30 parts of corn, 1 part of calcium carbonate, 60 parts of water, rice
100 parts, 60 parts of Chinese yam.The preparation method of solid fermentation culture medium: corn and water being mixed after impregnating 24 hours, then with it is big
Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Embodiment 2
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of tremella and corn, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) tremella strain is inoculated in potato glucose slant medium
In, after cultivating 10d at 20 DEG C, tremella slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) tremella bacterium is cultivated: tremella slant strains made from step (1) are cut into the fungus block of 10 pieces of 10 × 10mm or so,
1 piece of picking is inoculated into tremella bacterium solid seed culture medium, and after cultivating 40d at 25 DEG C, tremella bacterium solid spawn is made；Wherein,
Tremella bacterium solid seed culture medium composition are as follows: 60 parts of corn, 20 parts of wheat bran, 25 grams of maize peel, 10 parts of yeast extract, phosphoric acid
4 parts of potassium dihydrogen, 2 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then tremella bacterium solid bacterium made from inoculation step (2)
Kind (every 100g solid fermentation inoculation of medium 10g tremella bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly
13cm, width 90cm, under conditions of 28 DEG C of temperature, ventilatory capacity are 0.25:1v/v/m in solid state fermentation culturing room after culture 9d,
90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 45 parts of corn, 5 parts of calcium carbonate, 105 parts of water, rice
100 parts, 40 parts of Chinese yam.The preparation method of solid fermentation culture medium: corn and water being mixed after impregnating 24 hours, then with it is big
Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Embodiment 3
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of tremella and corn, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) tremella strain is inoculated in potato glucose slant medium
In, after cultivating 10d at 20 DEG C, tremella slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) tremella bacterium is cultivated: tremella slant strains made from step (1) being cut into the fungus block of 7 pieces of 10 × 10mm or so, are chosen
It takes 1 piece to be inoculated into tremella bacterium solid seed culture medium, after cultivating 40d at 25 DEG C, tremella bacterium solid spawn is made；Wherein, silver-colored
Ear bacterium solid seed culture medium composition are as follows: 60 parts of corn, 10 parts of wheat bran, 13 grams of maize peel, 1 part of yeast extract, di(2-ethylhexyl)phosphate
1 part of hydrogen potassium, 1 part of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then tremella bacterium solid bacterium made from inoculation step (2)
Kind (every 100g solid fermentation inoculation of medium 10g tremella bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly
20cm, width 60cm cultivate 15d under conditions of 20 DEG C of temperature, ventilatory capacity are 0.05:1v/v/m in solid state fermentation culturing room
Afterwards, 90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 60 parts of corn, 3 parts of calcium carbonate, 150 parts of water, rice
100 parts, 20 parts of Chinese yam.The preparation method of solid fermentation culture medium: corn and water being mixed after impregnating 24 hours, then with it is big
Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Performance test:
1. blood sugar reducing food made from embodiment 1-3 to be carried out to the content measuring of saponin(e, test method is as follows:
A. standard curve making: 0.1,0.15,0.20,0.25,0.30,0.35 and 0.40mL of titer 1mg/mL is drawn
The Dioscin standard solution configured with dehydrated alcohol, is respectively placed in 10mL tool plug test tube, and 70 DEG C of water-baths volatilize solvent, is added
5% vanillic aldehyde-the glacial acetic acid solution of 0.2mL, adds the perchloric acid of 0.8mL, shakes up, and heats 20min in 60 DEG C of water-baths,
Taking-up is cooled to room temperature with flowing water immediately, and glacial acetic acid 5mL is added, shakes up, its light absorption value OD is measured at 505nm.Draw Chinese yam
Saponin absorbance-concentration standard curve.
B. the extraction of sample dioscin: blood sugar reducing food 2.5g made from Example 1-3 respectively, with ether through Soxhlet
After extractor degreasing air-dries, the blood sugar reducing food 0.2g after degreasing air-dries is accurately weighed in 10mL tool plug test tube, it is accurate to be added
4mL contains 1% ammonium hydroxide ethyl alcohol, and ultrasound 1h in ultrasonoscope is put into after jumping a queue, test tube is then taken out, is placed in a centrifuge with 3000
Rev/min centrifugation 15min, supernatant is as prepare liquid.To pulpous state after leaching liquor reduced pressure, then it is dried under vacuum to perseverance for 30 DEG C
Weight, 95% alcohol melt again, filter, filtrate is dioscin extracting solution.
C. in sample dioscin concentration measurement: draw each 0.4mL of dioscin extracting solution, 3 repetitions are respectively placed in
In 10mL tool plug test tube, 70 DEG C of water-baths volatilize solvent, and the 5% vanillic aldehyde-glacial acetic acid solution of 0.2mL is added, adds 0.8mL
Perchloric acid, shake up, heat 20min in 60 DEG C of water-baths, taking-up is cooled to room temperature with flowing water immediately, be added glacial acetic acid 5mL, shake
It is even, its light absorption value OD is measured at 505nm.
2. blood sugar reducing food made from embodiment 1-3 to be carried out to the content measuring of polysaccharide, test method is as follows:
(1) polysaccharide directrix curve measures: accurately measuring the grape of 0.1,0.2,0.3,0.4,0.5,0.6 and 0.7mL1mg/mL
For saccharide solution in quantitative scale test tube, distilled water constant volume to 2mL is separately added into the phenol solution of 1.0mL 5%, quickly
The 5.0mL concentrated sulfuric acid is added and shakes up, boiling water bath 15min, circulating water is cooled to room temperature, and replaces titer with 2mL distilled water, cooling
Afterwards, water is used to replace Standard glucose solution as blank control.Light absorption value is measured at wavelength 490nm, then draws standard Portugal
Grape sugar concentration-absorbance curve figure.
(2) extraction of polysaccharide: weighing blood sugar reducing food made from 1g embodiment 1-3 respectively, is put into test tube, and 10mL is added and steams
Distilled water fills in test tube plug, the water-bath 3h at 80 DEG C, is transferred to centrifuge tube centrifugation, and residue adds 30mL distilled water, boiling water bath 1h,
Supernatant is taken, distilled water is settled to 10mL and is stored in 4 DEG C of refrigerators, for analyzing polyoses content.
(3) polysaccharide determination in sample: it is accurate to measure polysaccharide extraction liquid 2mL, it rapidly joins the 5.0mL concentrated sulfuric acid and shakes up, boil
Water-bath 15min, circulating water are cooled to room temperature, and replace titer to replace standard glucose molten with water after cooling with 2mL distilled water
Liquid is as blank control.Light absorption value is measured at wavelength 490nm.
The test result of saponin(e and polyoses content is shown in Table 1:
Table 1
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Saponin(e, mg/g blood sugar reducing food | 2.0 | 5.6 | 10 |
| Polysaccharide, mg/g blood sugar reducing food | 30 | 15.2 | 2.1 |
3. function of blood sugar reduction is tested
Test method: 100 mouse are adapted to after feeding 5d with basal feed, and four oxygen that 180mg/kg weight is injected intraperitoneally are phonetic
Pyridine feeds common mouse feed (12% casein, 60.98% cornstarch, 15% sucrose, 7% corn oil, 1% vitamin sugar
Powder, 4% mineral salt, 0.02% cod-liver oil) after five days, take tail blood blood sugar kit to measure blood-sugar content, with blood-sugar content height
6 groups are randomly divided into the model mouse of 13mmol/mL: Normal group, hyperglycemia control group, 1 sample sets of embodiment, embodiment 2
3 sample sets of sample sets and embodiment, all Normal groups and hyperglycemia control group experimental mouse all feed basal feed, embodiment
Sample sets feed the blood sugar reducing food of+30% embodiment 1 of 60% basal feed or embodiment 2 or embodiment 3 respectively.Each group mouse
Freely ingests and drink water, 18~22 DEG C of animal room temperature.After successive administration four weeks, blood is taken every seven days tail veins, is analyzed
Blood-sugar content, test result are shown in Fig. 1.
The change of blood sugar curve graph of mouse, can be seen by Fig. 1 after the blood sugar reducing food that Fig. 1 is feeding addition embodiment 1-3
Out, during entirely test, normal group and hyperglycemia control group blood glucose value are basically unchanged, and embodiment 1, embodiment 2 and implementation
3 groups of blood glucose values of example significantly reduce.
Claims (6)
1. a kind of method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn, which is characterized in that including as follows
Step:
(1) test tube expands culture: tremella strain being inoculated in potato glucose slant medium, cultivates 8- at 20-30 DEG C
After 10d, tremella slant strains, 10 DEG C of preservations are made；
(2) tremella bacterium is cultivated: tremella slant strains made from step (1) being cut into 7-10 block, 1 piece of picking is inoculated into tremella bacterium and consolidates
In body seed culture medium, after cultivating 35-40d at 25-30 DEG C, tremella bacterium solid spawn is made；Wherein, tremella bacterium solid seed is trained
Support base composition are as follows: 30-60 parts of corn, 10-30 parts of wheat bran, 13-38 parts of maize peel, 1-10 parts of yeast extract, biphosphate
1-5 parts of potassium, 1-5 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, and then tremella bacterium solid spawn made from inoculation step (2), mixes
It is divided after even on solid state fermentation bed, thickness 6-20cm, width 60-120cm, after cultivating 3-15d in solid state fermentation culturing room,
It is dry to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 100 parts of rice, 30-60 parts of corn, 20-60 parts of Chinese yam, carbon
Sour calcium 0.5-5 parts, 60-150 parts of water.
2. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn as described in claim 1, feature
It is, in step (1), potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water
1000mL。
3. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn as described in claim 1, feature
It is, in step (2), tremella bacterium consolidates seed culture medium composition are as follows: 60 parts of corn, 20 parts of wheat bran, 25 grams of maize peel, yeast
10 parts of medicinal extract, 4 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 110 parts of water.
4. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn as described in claim 1, feature
It is, in step (3), every 100g solid fermentation inoculation of medium 1-20g tremella bacterium solid spawn.
5. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn as described in claim 1, feature
It is, in step (3), the preparation method of the solid fermentation culture medium: corn and water being mixed after impregnating 24 hours, and big
Rice, Chinese yam mixing, add calcium carbonate, after mixing 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
6. as described in claims 1 or 2 or 3 or 4 or 5 prepare blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn
Method, which is characterized in that in step (3), the condition of solid state fermentation culture are as follows: 20-35 DEG C of temperature, ventilatory capacity 0.05-0.5:
1v/v/m。
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