CN109259198A - The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder - Google Patents

The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder Download PDF

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CN109259198A
CN109259198A CN201811204527.0A CN201811204527A CN109259198A CN 109259198 A CN109259198 A CN 109259198A CN 201811204527 A CN201811204527 A CN 201811204527A CN 109259198 A CN109259198 A CN 109259198A
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张志才
王萍
梅延安
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Zhenjiang Yemaikang Food Biotechnology Co Ltd
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Abstract

The present invention relates to a kind of methods for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, using rice, bean cake powder, Chinese yam as primary raw material, urea is auxiliary material, it is mixed after sterilizing with cicada fungus bacterium, it divides on solid state fermentation bed, solid state fermentation is carried out, obtained fermentation material is blood sugar reducing food.Of the invention every gram of blood sugar reducing food contains 2~30mg of polysaccharide, 2~10mg of ergosterol, blood glucose can be significantly reduced in the blood sugar reducing food, it is a kind of pure natural preparation, the not only quick, significant effect compared with existing hypoglycemic medicine, and not will lead to hypoglycemia after taking.

Description

The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder
Technical field
The present invention relates to a kind of methods for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, belong to Technical field of bioengineering.
Background technique
It is especially high with China's expanding economy, the raising of living standards of the people and the variation of people's dietary structure Rouge dietary int ake increases, and the disease incidence of the " three high " disease such as hypertension, hyperlipidemia and hyperglycemia rises year by year.Control and prevention " three high ", although drug effect is preferable, side effect is big, price is relatively high, and not each patient can receive, therefore develops The food that curative effect is better, has no toxic side effect has good development prospect.
Chinese yam is the cultivar that underground meat stem tuber can be formed in potato preparatory course Dioscorea, annual or perennial prehensile rattan This plant, alias Chinese yam, potato damage, RHIIZOMA DIOSCOREAE from Henan of China etc..Chinese yam is a kind of highly efficient and productive industrial crops of dietotherapeutic, drying to be used as medicine For tonic invigorator, there is auxiliary curative effect to weakness, chronic enteritis, diabetes etc..Chinese yam is rich in protein, amino acid, starch etc. Nutriment and polysaccharide, dioscin (steroid saponin), vitamin, allantoin, dopamine, Chinese yam alkali, polyphenol oxidase etc. Bioactive ingredients.The initial eating method of Chinese yam is long term storage after removing the peel, dry, being milled, and yam flour is usually passed through boiling It is made into various food or directly stews Chinese yam at soup and eat.In recent years, people are to Chinese yam with the progress of science and technology Exploitation also gradually to in-depth development, there are many report in relation to Chinese yam product R & D: Chinese yam convection drying is made Quan Fen, fine powder, Chinese yam are directly beaten, series beverage are made, and ourishing rice flour is made in Chinese yam starch, by Chinese yam and pure milk, rice Equal composite fermentations are at yam Yogurt and yam wine etc..But unstable quality, and Chinese yam also has the general character of Chinese medicine, slow effect. Mountain the effective elements of the medicine is directly extracted to exploitation and is increasingly becoming Chinese yam development and utilization at food with health care function New approaches.
Cicada fungus (Cordyceps cicadae Shing) is to colonize in a kind of fungi of cicada body, ascocarp and cicada body portion It is collectively referred to as golden cicada flower, only made some pharmaceutical chemistry and pharmacological research to cicada fungus both at home and abroad, such as researches show that cicada fungus to have anti-swell Tumor, anti-inflammatory, immunological regulation and calm effect.Research both at home and abroad focuses primarily upon the polysaccharide of cicada fungus bacterium fermentation and nucleosides is sent out The effect of ferment, Study on extraction and solid-state, liquid state fermentation product, is analyzed.Reported literature it is more be kidney trouble effect Using and Mechanism Study.If CSM treated alleviates the damage of glomerulus, the renal function and kidney failure complication of animal are improved (anaemia, malnutrition), has delayed the process of glomerulus, and mechanism may have the expression for lowering PAI-1, make glomerulus hair The expression of the thin upper u-PA of blood vessel button loop is accordingly increased, and the expression quantity of Col- IV in glomerulus is reduced.Simultaneously also studies have reported that cicada Flower fungus extract cyclodepsipeptide cordycecin, beauvericin have effects that inhibition anti-liver cancer and anti-cell. Cicada fungus fungus extract Ergosterol peroxide (EP), (5,8-epidioxy-22E-ergosta-6,22-dien-3-ol) Protection renal fibrosis has obvious effect.But up to the present without utilizing food of the production containing ergosterol and polysaccharide Report, timely Liquid Culture are also all laboratory scales, and can technology be used to industrialize and can't determine.
Summary of the invention
The solid state transformed Chinese yam of cicada fungus bacterium and dregs of beans are utilized present invention aims to solve the deficiencies of the prior art, and provides a kind of a kind of The method that powder prepares blood sugar reducing food.
Technical solution
Cicada fungus bacterium is expanded culture technique and combined with solid-state bioconversion by the present inventor, using cicada fungus bacterium as strain, Chinese yam, Bean cake powder and rice are primary raw material, are combined using fermentation with conversion, produce the food with function of blood sugar reduction.Specific side Case is as follows:
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) Cordyceps cicadae strain is inoculated in potato glucose slant medium In, after cultivating 8-10d at 20-30 DEG C, cicada fungus bacterium slant strains, 10 DEG C of preservations are made；
(2) cicada fungus bacterium is cultivated: cicada fungus bacterium inclined-plane made from step (1) is cut into the fungus block of 10 × 10mm of 7-10 block or so, 1 piece of picking is inoculated into cicada fungus bacterium solid seed culture medium, and after cultivating 35-40d at 25-30 DEG C, cicada fungus bacterium solid spawn is made； Wherein, cicada fungus bacterium solid seed culture medium forms are as follows: and 30-60 parts of bean cake powder, 10-30 parts of wheat bran, cicada pupa dry powder 13-38 Part, 1-10 parts of yeast extract, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, and then Cordyceps cicadae strain made from inoculation step (2), is mixed thoroughly After divide on solid state fermentation bed, thickness 6-20cm, width 60-120cm, in solid state fermentation culturing room cultivate 3-15d after, do It is dry to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 100 parts of rice, 30-60 parts of bean cake powder, Chinese yam 20- 60 parts, 0.5-5 parts of urea, 60-150 parts of water.
Further, in step (1), potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, fine jade 20 grams of rouge, water 1000mL.
Further, in step (3), every 100g solid fermentation inoculation of medium 1-20g cicada fungus bacterium solid spawn.
Further, in step (3), the preparation method of the solid fermentation culture medium: bean cake powder and water is mixed and impregnate 24 Hour after, mix with rice, Chinese yam, adds urea, after mixing 120 DEG C sterilizing 2 hours, be cooled to room temperature to get.
Further, in step (3), the condition of solid state fermentation culture are as follows: 20-35 DEG C of temperature, ventilatory capacity 0.05-0.5: 1v/v/m。
The utility model has the advantages that the present invention prepares blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, and with Bean cake powder, rice and Chinese yam significantly improve cicada fungus granulose and wheat in product to add urea in the solid medium of matrix The content of angle sterol makes polysaccharide and Quantitative Determination of Ergosterol increase separately 20% and 25%, to significantly enhance the drop blood of product Sugared function；Blood sugar reducing food of the invention is a kind of pure natural preparation, every gram containing 2~30mg of polysaccharide, ergosterol 2~ 10mg, the not only quick, significant effect compared with existing hypoglycemic medicine, and not will lead to hypoglycemia after taking, without any pair Effect.
Detailed description of the invention
The change of blood sugar curve graph of mouse after the blood sugar reducing food that Fig. 1 is feeding addition embodiment 1-3.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples.
Embodiment 1
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) Cordyceps cicadae strain is inoculated in potato glucose slant medium In, after cultivating 10d at 20 DEG C, cicada fungus bacterium slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) cicada fungus bacterium is cultivated: cicada fungus bacterium slant strains made from step (1) are cut into the fungus block of 8 pieces of 10 × 10mm or so, 1 piece of picking is inoculated into cicada fungus bacterium solid seed culture medium, and after cultivating 40d at 25 DEG C, cicada fungus bacterium solid spawn is made；Wherein, Cicada fungus bacterium solid seed culture medium composition are as follows: 30 parts of bean cake powder, 30 parts of wheat bran, 38 parts of cicada pupa dry powder, 10 parts of yeast extract, 4 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then cicada fungus bacterium solid bacterium made from inoculation step (2) Kind (every 100g solid fermentation inoculation of medium 1g cicada fungus bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly 6cm, width 120cm, under conditions of 35 DEG C of temperature, ventilatory capacity are 0.5:1v/v/m in solid state fermentation culturing room after culture 3d, 90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 30 parts of bean cake powder, 1 part of urea, 60 parts of water, rice 100 parts, 60 parts of Chinese yam.The preparation method of solid fermentation culture medium: bean cake powder and water being mixed after impregnating 24 hours, then with it is big Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Embodiment 2
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) Cordyceps cicadae strain is inoculated in potato glucose slant medium In, after cultivating 10d at 20 DEG C, cicada fungus bacterium slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) cicada fungus bacterium is cultivated: cicada fungus bacterium slant strains made from step (1) are cut into the bacterium of 10 pieces of 10 × 10mm or so Block, 1 piece of picking is inoculated into cicada fungus bacterium solid seed culture medium, and after cultivating 40d at 25 DEG C, cicada fungus bacterium solid spawn is made；Its In, cicada fungus bacterium solid seed culture medium composition are as follows: 60 parts of bean cake powder, 20 parts of wheat bran, 25 grams of cicada pupa dry powder, yeast extract 10 Part, 4 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then cicada fungus bacterium solid bacterium made from inoculation step (2) Kind (every 100g solid fermentation inoculation of medium 10g cicada fungus bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly 13cm, width 90cm, under conditions of 28 DEG C of temperature, ventilatory capacity are 0.25:1v/v/m in solid state fermentation culturing room after culture 9d, 90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 45 parts of bean cake powder, 5 parts of urea, 105 parts of water, rice 100 parts, 40 parts of Chinese yam.The preparation method of solid fermentation culture medium: bean cake powder and water being mixed after impregnating 24 hours, then with it is big Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Embodiment 3
A method of blood sugar reducing food being prepared using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, is included the following steps:
(1) test tube expands culture: a fritter (4 × 4mm) Cordyceps cicadae strain is inoculated in potato glucose slant medium In, after cultivating 10d at 20 DEG C, cicada fungus bacterium slant strains, 10 DEG C of preservations are made；
Potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, 20 grams of agar, water 1000mL.
(2) cicada fungus bacterium is cultivated: cicada fungus bacterium slant strains made from step (1) are cut into the fungus block of 7 pieces of 10 × 10mm or so, 1 piece of picking is inoculated into cicada fungus bacterium solid seed culture medium, and after cultivating 40d at 25 DEG C, cicada fungus bacterium solid spawn is made；Wherein, Cicada fungus bacterium solid seed culture medium composition are as follows: 60 parts of bean cake powder, 10 parts of wheat bran, 13 grams of cicada pupa dry powder, 1 part of yeast extract, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, then cicada fungus bacterium solid bacterium made from inoculation step (2) Kind (every 100g solid fermentation inoculation of medium 10g cicada fungus bacterium solid spawn), is divided on solid state fermentation bed, thickness after mixing thoroughly 20cm, width 60cm cultivate 15d under conditions of 20 DEG C of temperature, ventilatory capacity are 0.05:1v/v/m in solid state fermentation culturing room Afterwards, 90 DEG C of dryings to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 60 parts of bean cake powder, 3 parts of urea, 150 parts of water, rice 100 parts, 20 parts of Chinese yam.The preparation method of solid fermentation culture medium: bean cake powder and water being mixed after impregnating 24 hours, then with it is big Rice, Chinese yam mixing, then 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
Performance test:
1. blood sugar reducing food made from embodiment 1-3 to be carried out to the content measuring of ergosterol, test method is as follows:
(1) it chromatographic condition: uses Agilent Eclipse XDB-C18 chromatographic column (250mm × 4.6mm, 5 μm)；Flowing Mutually select methanol；Type of elution is isocratic elution；Volume flow 1.0mL/min；35 DEG C of column temperature；10 μ L of sample volume；Detection wavelength 281nm。
(2) standard curve making: it is appropriate that precision weighs 10mg Ergosterol reference substance, is placed in 25mL measuring bottle, adds methanol Scale is dissolved and be diluted to, the Ergosterol reference substance stock solution to get 0.4mg/mL is shaken up.Ergosterol reference substance is stored up It to get mass concentration is 0.2 that standby liquid uses 2,4,8,16,32,64 times of methanol dilution respectively, 0.1,0.05,0.025,0.0125, The Ergosterol reference substance solution of 0.00625 μ g/mL, each precision 10 μ L of sample introduction, measures peak area by above-mentioned chromatographic condition.It draws Peak area-ergosterol mass concentration standard curve.
(3) test sample Quantitative Determination of Ergosterol measures: the cicada fungus culture test sample about 0.2g of drying is weighed, it is accurately weighed. It is placed in 25mL stuffed conical flask, precision pipettes petroleum ether 6mL, ultrasonic 30min, takes out, and 4 000r/min are centrifuged 10min, takes Appropriate supernatant 3mL is volatilized on 55 DEG C of thermostat water baths, is volatilized rear residue and is sufficiently dissolved with methanol, and is settled to 10mL measuring bottle In.Supernatant is taken, crosses 0.22 μm of miillpore filter to get test solution.Accurate 10 μ L of sample introduction is measured by above-mentioned chromatographic condition Peak area.According to peak area-ergosterol mass concentration standard curve, Quantitative Determination of Ergosterol in sample is calculated.
2. blood sugar reducing food made from embodiment 1-3 to be carried out to the content measuring of polysaccharide, test method is as follows:
(1) polysaccharide directrix curve measures: accurately measuring the grape of 0.1,0.2,0.3,0.4,0.5,0.6 and 0.7mL1mg/mL For saccharide solution in quantitative scale test tube, distilled water constant volume to 2mL is separately added into the phenol solution of 1.0mL 5%, quickly The 5.0mL concentrated sulfuric acid is added and shakes up, boiling water bath 15min, circulating water is cooled to room temperature, and replaces titer with 2mL distilled water, cooling Afterwards, water is used to replace Standard glucose solution as blank control.Light absorption value is measured at wavelength 490nm, then draws standard Portugal Grape sugar concentration-absorbance curve figure.
(2) extraction of polysaccharide: weighing blood sugar reducing food made from 1g embodiment 1-3 respectively, is put into test tube, and 10mL is added and steams Distilled water fills in test tube plug, the water-bath 3h at 80 DEG C, is transferred to centrifuge tube centrifugation, and residue adds 30mL distilled water, boiling water bath 1h, Supernatant is taken, distilled water is settled to 10mL and is stored in 4 DEG C of refrigerators, for analyzing polyoses content.
(3) polysaccharide determination in sample: it is accurate to measure polysaccharide extraction liquid 2mL, it rapidly joins the 5.0mL concentrated sulfuric acid and shakes up, boil Water-bath 15min, circulating water are cooled to room temperature, and replace titer to replace standard glucose molten with water after cooling with 2mL distilled water Liquid is as blank control.Light absorption value is measured at wavelength 490nm.
The test result of ergosterol and polyoses content is shown in Table 1:
Table 1
Embodiment 1 Embodiment 2 Embodiment 3
Ergosterol, mg/g blood sugar reducing food 2.0 5.6 10
Polysaccharide, mg/g blood sugar reducing food 30 15.2 2.1
3. function of blood sugar reduction is tested
Test method: 100 mouse are adapted to after feeding 5d with basal feed, and four oxygen that 180mg/kg weight is injected intraperitoneally are phonetic Pyridine feeds common mouse feed (12% casein, 60.98% cornstarch, 15% sucrose, 7% corn oil, 1% vitamin sugar Powder, 4% mineral salt, 0.02% cod-liver oil) after five days, take tail blood blood sugar kit to measure blood-sugar content, with blood-sugar content height 6 groups are randomly divided into the model mouse of 13mmol/mL: Normal group, hyperglycemia control group, 1 sample sets of embodiment, embodiment 2 3 sample sets of sample sets and embodiment, all Normal groups and hyperglycemia control group experimental mouse all feed basal feed, embodiment Sample sets feed the blood sugar reducing food of+30% embodiment 1 of 60% basal feed or embodiment 2 or embodiment 3 respectively.Each group mouse Freely ingests and drink water, 18~22 DEG C of animal room temperature.After successive administration four weeks, blood is taken every seven days tail veins, is analyzed Blood-sugar content, test result are shown in Fig. 1.
The change of blood sugar curve graph of mouse, can be seen by Fig. 1 after the blood sugar reducing food that Fig. 1 is feeding addition embodiment 1-3 Out, during entirely test, normal group and hyperglycemia control group blood glucose value are basically unchanged, and embodiment 1, embodiment 2 and implementation 3 groups of blood glucose values of example significantly reduce.

Claims (6)

1. a kind of method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder, which is characterized in that including Following steps:
(1) test tube expands culture: Cordyceps cicadae strain being inoculated in potato glucose slant medium, cultivates 8- at 20-30 DEG C After 10d, cicada fungus bacterium slant strains, 10 DEG C of preservations are made；
(2) cicada fungus bacterium is cultivated: cicada fungus bacterium slant strains made from step (1) being cut into 7-10 block, 1 piece of picking is inoculated into cicada fungus bacterium In solid seed culture medium, after cultivating 35-40d at 25-30 DEG C, cicada fungus bacterium solid spawn is made；Wherein, cicada fungus bacterium solid seed Culture medium composition are as follows: 30-60 parts of bean cake powder, 10-30 parts of wheat bran, 13-38 parts of cicada pupa dry powder, 1-10 parts of yeast extract, phosphorus 1-5 parts of acid dihydride potassium, 1-5 parts of magnesium sulfate, 110 parts of water；
(3) solid fermentation culture: preparing solid fermentation culture medium, and then cicada fungus bacterium solid spawn made from inoculation step (2), mixes It is divided after even on solid state fermentation bed, thickness 6-20cm, width 60-120cm, after cultivating 3-15d in solid state fermentation culturing room, It is dry to constant weight to get；
In step (3), the formula of the solid fermentation culture medium are as follows: 100 parts of rice, 30-60 parts of bean cake powder, 20-60 parts of Chinese yam, 0.5-5 parts of urea, 60-150 parts of water.
2. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder as described in claim 1, It is characterized in that, in step (1), potato glucose slant medium composition: 200 grams of potato, 20 grams of glucose, agar 20 Gram, water 1000mL.
3. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder as described in claim 1, It is characterized in that, in step (2), cicada fungus bacterium consolidates seed culture medium composition are as follows: 60 parts of bean cake powder, 20 parts of wheat bran, cicada pupa dry powder 25 grams, 10 parts of yeast extract, 4 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 110 parts of water.
4. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder as described in claim 1, It is characterized in that, in step (3), every 100g solid fermentation inoculation of medium 1-20g cicada fungus bacterium solid spawn.
5. the method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder as described in claim 1, It being characterized in that, in step (3), the preparation method of the solid fermentation culture medium: bean cake powder and water being mixed after impregnating 24 hours, Mixed with rice, Chinese yam, add urea, after mixing 120 DEG C sterilize 2 hours, be cooled to room temperature to get.
6. preparing hypoglycemic food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder as described in claims 1 or 2 or 3 or 4 or 5 The method of product, which is characterized in that in step (3), the condition of solid state fermentation culture are as follows: 20-35 DEG C of temperature, ventilatory capacity 0.05- 0.5:1v/v/m。
CN201811204527.0A 2018-10-16 2018-10-16 The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder Withdrawn CN109259198A (en)

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